SitesBLAST
Comparing WP_017537197.1 NCBI__GCF_000341205.1:WP_017537197.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 14 hits to proteins with known functional sites (download)
5ol2B The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
34% identity, 95% coverage: 2:242/254 of query aligns to 7:247/260 of 5ol2B
- binding flavin-adenine dinucleotide: I7 (≠ V2), K8 (= K3), D38 (= D34), M61 (= M57), A92 (≠ S88), D93 (= D89), T94 (≠ A90), T97 (= T93), A115 (≠ L111), G116 (= G112), A119 (≠ S115), G122 (≠ A118), T124 (= T120), A125 (≠ G121), V127 (= V123)
Sites not aligning to the query:
6fahB Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
35% identity, 91% coverage: 3:234/254 of query aligns to 8:240/262 of 6fahB
- binding flavin-adenine dinucleotide: K8 (= K3), D38 (= D34), T61 (= T56), M62 (= M57), A93 (≠ S88), D94 (= D89), T95 (≠ A90), T98 (= T93), L102 (= L97), G118 (= G112), Q120 (≠ E114), G124 (≠ A118), T126 (= T120), A127 (≠ G121), V129 (= V123), T223 (= T217)
Sites not aligning to the query:
7koeA Electron bifurcating flavoprotein fix/etfabcx (see paper)
31% identity, 100% coverage: 1:253/254 of query aligns to 7:267/282 of 7koeA
- binding flavin-adenine dinucleotide: C7 (≠ M1), I8 (≠ V2), K9 (= K3), D39 (= D34), M62 (= M57), A93 (≠ S88), D94 (= D89), T95 (≠ A90), T98 (= T93), L102 (= L97), T121 (≠ L111), G122 (= G112), A125 (≠ S115), G128 (≠ A118), T130 (= T120), G131 (= G121), V133 (= V123)
4l2iB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
34% identity, 100% coverage: 2:254/254 of query aligns to 7:261/263 of 4l2iB
- binding flavin-adenine dinucleotide: V7 (= V2), K8 (= K3), Y37 (≠ L33), D38 (= D34), Y40 (= Y36), T60 (= T56), M61 (= M57), A92 (≠ S88), D93 (= D89), T94 (≠ A90), T97 (= T93), M101 (≠ L97), C116 (≠ L111), G117 (= G112), Q119 (≠ E114), A120 (≠ S115), G123 (≠ A118), T125 (= T120), A126 (≠ G121), V128 (= V123)
- binding nicotinamide-adenine-dinucleotide: M61 (= M57), G62 (= G58), P63 (= P59), S85 (≠ V81), D86 (= D82), G91 (= G87)
Sites not aligning to the query:
4kpuB Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
34% identity, 100% coverage: 2:254/254 of query aligns to 7:261/263 of 4kpuB
- binding flavin-adenine dinucleotide: V7 (= V2), K8 (= K3), Y37 (≠ L33), D38 (= D34), Y40 (= Y36), M61 (= M57), A92 (≠ S88), D93 (= D89), T94 (≠ A90), T97 (= T93), C116 (≠ L111), G117 (= G112), Q119 (≠ E114), A120 (≠ S115), G123 (≠ A118), T125 (= T120), A126 (≠ G121), V128 (= V123)
Sites not aligning to the query:
1efpB Electron transfer flavoprotein (etf) from paracoccus denitrificans (see paper)
36% identity, 81% coverage: 2:206/254 of query aligns to 7:214/246 of 1efpB
- binding adenosine monophosphate: V7 (= V2), D39 (= D34), I63 (≠ M57), V101 (≠ T93), A119 (≠ L111), G120 (= G112), Q122 (≠ E114), A123 (≠ S115), N126 (≠ A118), M128 (≠ T120), N129 (≠ G121), T131 (≠ V123)
- binding flavin-adenine dinucleotide: Y13 (≠ T8), L182 (≠ E174)
Sites not aligning to the query:
7qh2B Cryo-em structure of ldh-etfab complex from acetobacterium woodii (see paper)
30% identity, 100% coverage: 2:254/254 of query aligns to 8:264/265 of 7qh2B
- binding flavin-adenine dinucleotide: K9 (= K3), D39 (= D34), M62 (= M57), D94 (= D89), V95 (≠ A90), L102 (= L97), G118 (= G112), Q120 (≠ E114), T121 (≠ S115), T126 (= T120), A127 (≠ G121), V129 (= V123)
Sites not aligning to the query:
Q2TBV3 Electron transfer flavoprotein subunit beta; Beta-ETF from Bos taurus (Bovine) (see paper)
34% identity, 97% coverage: 2:247/254 of query aligns to 10:252/255 of Q2TBV3
- K200 (≠ Q189) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K192) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
Q9DCW4 Electron transfer flavoprotein subunit beta; Beta-ETF from Mus musculus (Mouse) (see paper)
34% identity, 95% coverage: 2:243/254 of query aligns to 10:248/255 of Q9DCW4
- K200 (≠ Q189) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate
- K203 (= K192) modified: N6,N6,N6-trimethyllysine; by ETFBKMT
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P38117 Electron transfer flavoprotein subunit beta; Beta-ETF from Homo sapiens (Human) (see 10 papers)
34% identity, 97% coverage: 2:247/254 of query aligns to 10:252/255 of P38117
- NPFC 39:42 (≠ NELD 31:34) binding AMP
- C66 (≠ M57) binding AMP
- 123:134 (vs. 112:123, 17% identical) binding AMP
- D128 (= D117) to N: in GA2B; decreased protein stability; dbSNP:rs104894678
- T154 (≠ D143) to M: in dbSNP:rs1130426
- R164 (= R153) to Q: in GA2B; reduced electron transfer activity; dbSNP:rs104894677
- E165 (≠ Q154) mutation E->A,Q: Drastically increases interprotein electron transfer rates.
- 183:205 (vs. 172:194, 48% identical) Recognition loop
- L195 (≠ F184) mutation to A: Severely impaired in complex formation with ACADM.
- K200 (≠ Q189) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; alternate; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- KAK 200:202 (≠ QAK 189:191) mutation to RAR: Does not abolish methylation by ETFBKMT.
- KAKK 200:203 (≠ QAKK 189:192) mutation to RAKR: Does not abolish electron transfer activity. Abolishes sensitivity to inhibition by lysine methyltransferase ETFBKMT.
- K203 (= K192) modified: N6,N6,N6-trimethyllysine; by ETFBKMT; mutation to R: Does not abolish electron transfer activity. Decreases sensitivity to inhibition by lysine methyltransferase ETFBKMT.
Sites not aligning to the query:
2a1uB Crystal structure of the human etf e165betaa mutant (see paper)
34% identity, 97% coverage: 2:247/254 of query aligns to 7:249/252 of 2a1uB
- binding adenosine monophosphate: V7 (= V2), K8 (= K3), C39 (≠ D34), C63 (≠ M57), V101 (≠ T93), L119 (= L111), G120 (= G112), Q122 (≠ E114), A123 (≠ S115), D126 (≠ A118), C128 (≠ T120), N129 (≠ G121), Q130 (≠ V122), T131 (≠ V123)
- binding flavin-adenine dinucleotide: P37 (≠ E32), L182 (≠ E174)
Sites not aligning to the query:
1o96A Structure of electron transferring flavoprotein for methylophilus methylotrophus. (see paper)
33% identity, 86% coverage: 30:248/254 of query aligns to 34:254/261 of 1o96A
- binding adenosine monophosphate: D38 (= D34), V63 (vs. gap), V99 (≠ T93), A117 (≠ L111), G118 (= G112), Q120 (≠ E114), S121 (= S115), Y126 (≠ T120), A127 (≠ G121), T129 (≠ V123)
- binding flavin-adenine dinucleotide: Q120 (≠ E114), Q182 (≠ V173)
Sites not aligning to the query:
P53570 Electron transfer flavoprotein subunit beta; Beta-ETF; Electron transfer flavoprotein small subunit; ETFSS from Methylophilus methylotrophus (Bacterium W3A1) (see paper)
33% identity, 81% coverage: 30:235/254 of query aligns to 35:244/264 of P53570
Sites not aligning to the query:
5ow0B Crystal structure of an electron transfer flavoprotein from geobacter metallireducens (see paper)
36% identity, 78% coverage: 1:198/254 of query aligns to 6:202/251 of 5ow0B
- binding adenosine monophosphate: L6 (≠ M1), A7 (≠ V2), K8 (= K3), D39 (= D34), Y62 (≠ M57), I95 (≠ T93), A114 (≠ L111), G115 (= G112), Q117 (≠ E114), S118 (= S115), R123 (≠ T120), G124 (= G121), V125 (= V122), V126 (= V123), P127 (≠ G124)
- binding flavin-adenine dinucleotide: L37 (≠ E32), Y38 (≠ L33)
Query Sequence
>WP_017537197.1 NCBI__GCF_000341205.1:WP_017537197.1
MVKQVPDTATERKLSSDDFTLDRAASDGVINELDEYAIEEALLLREKHGGEVTVLTMGPD
QATDSIRKALSMGADKAVHLVDDALHGSDALQTAYALSKALGTIEFDLVVLGSESTDART
GVVGAALAEYLGLPQLTLAGKVDVDGSAVTVQRQTDYGYDVVEAQLPAVVSVVEKINEPR
YPSFKLIMQAKKKPVAKLGIADAGIEAERVGTAAAGTETVEASPAPPRAAGTVVKDEGDG
GAKAADFLAEKKFL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory