SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
38% identity, 87% coverage: 8:258/288 of query aligns to 4:266/304 of Q96XT4

query
sites
Q96XT4

R

R

N

K

N

P

I

V

K

F

P

V

N

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

F

F

S

G

V

I

Q

L

A

R

A

A

I

E

Q

E

K

M

A

A

C

I

A

R

A

E

N

L

G

G

L

I

E

N

P

P

D

K

E

S

L

V

A

V

V

I

I

V

T

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

G

G

R

K

L

I

S

P

G

H

Y

F

-

M

-

N

I

L

K

P

S

I

Y

S

G

G

F

V

H

H

A

T

T

L

H

H

G

G

R

R

S

S

L

I

P

A

V

F

A

A

Q

T

G

G

V

I

K

K

M

L

A

S

N

N

D

P

E

S

L

L

T

E

V

V

I

I

A

V

S

N

G

V

G

G

D
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D

G
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G

D
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D

G

G

F

L

A

G

I

I

G

G

M

M

G

G

H

H

T

F

V

V

H

H

A

L

L

G

K

R

R

R

N

N

I

I

D

D

I

I

T

A

Y

V

I

L

V

V

M

H

D

N

N
|
N

Q

G

I
x
V

Y

Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

T

A

S

S

P

P

S

T

S

L

E

H

K

R

G

G

F

E

V

K

T

T

K

K

S

S

T

L

P

P

N

K

G

P

S

N

I

I

E

M

N

D

A

A

I

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Q

N

P

P

I

L

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A

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V

A

A

M

L

A

A

S

A

G

G

A

Y

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T

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F

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A

A

Q

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Y

S

A

S

Y

D

D

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M

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L

L

T

K

A

E

I

L

I

I

Q

K

E

K

A

A

M

I

N

L

H

H

K

K

G

G

F

S

S

A

F

L

V

V

N

D

V

I

F

L

S

Q

P

P

C
|
C

V

P

T

T

Y

Y

N

N

K

D

V

I

N

N

T

T

Y

K

D

E

W

W

F

Y

K

D

E

K

N

R

L

V

T

Y

A

K

V

L

E

D

D

N

I

V

G

P

D

G

Y

W

D

D

N

P

T

V

D

V

Q

R

K

K

-

E

-

A

-

Q

-

K

-

F

-

E

-

Q

A

A

A

I

M

M

N

K

H

S

I

Y

M

E

E

W

H

G

E

E

G

K

L

I

L

P

K

I

G

G

I

I

I

F

Y

Y

Q

Q

N

N

K

E

E

L

A

V

S

P

S

T

Y

F

Q

E

E

D

C12 (= C16) binding [4Fe-4S] cluster
C15 (= C19) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 48:51) binding thiamine diphosphate
C46 (= C50) binding [4Fe-4S] cluster
D90 (= D92) binding Mg(2+)
GD 91:92 (= GD 93:94) binding thiamine diphosphate
N118 (= N120) binding Mg(2+)
V120 (≠ I122) binding Mg(2+)
GL 122:123 (= GL 124:125) binding thiamine diphosphate
C197 (= C199) binding [4Fe-4S] cluster

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
38% identity, 97% coverage: 1:278/288 of query aligns to 1:288/305 of Q96Y68

query
sites
Q96Y68

M

M

A

A

T

A

F

F

K

K

D

P

F

Q

R

W

N

N

N

D

I

-

K

-

P

-

N

-

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

F

F

S

G

V

I

Q

L

A

N

A

A

I

E

Q

Q

K

Q

A

A

C

I

A

V

A

E

N

L

G

G

L

V

E

D

P

T

D

K

E

N

L

V

A

V

V

V

I

V

T

S

G

G

I

I

G

G

C
|
C

S

S

G

G

R
x
K

L

I

S

P

G

H

Y

F

I

F

K

R

S

T

-

P

-

I

Y

S

G

G

F

V

H

H

A

T

T

L

H

H

G

G

R

R

S

A

L

I

P

A

V

F

A

A

Q

T

G

G

V

I

K

K

M

L

A

S

N

N

D

P

E

D

L

L

T

V

V

V

I

I

A

V

S

N

G

G

D
|
D

G
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G

D
|
D

G

L

F

L

A

G

I

I

G

G

M

A

G

G

H

H

T

F

V

V

H

A

A

A

L

G

K

R

R

R

N

N

I

V

D

D

I

M

T

V

Y

V

I

I

V

L

M

H

D

D

N
|
N

Q

G

I
x
V

Y

Y

G
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G

L
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L

T

T

K
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K

G

G

Q

Q

T

A

S

S

P

P

S

T

S

L

E

K

K

R

G

G

F

E

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K

T

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K

K

S

S

T

L

P

P

N

R

G

P

S

N

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N

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A

A

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P

I

I

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T

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A

A

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A

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F

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A

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Y

D

D

I

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K
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K

Q

H

L

L

T

K

A

E

I

L

I

I

Q

K

E

S

A

A

M

I

N

K

H

H

K

K

G

G

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S

A

F

L

V

I

N

D

V

V

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S

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C
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C

V

P

T

T

Y

Y

N

N

K

D

V

I

N

N

T

T

Y

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E

W

W

F

Y

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D

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K

N

R

L

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Y

A

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L

E

D

D

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I

L

G

P

D

D

Y

W

D

D

-

P

-

V

-

K

-

P

-

E

-

V

N

N

T

E

D

K

Q

I

K

K

A

R

A

A

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I

N

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H

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M

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E

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H

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-

G

E

D

G

R

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G

G

I

I

I

F

Y

Y

Q

Q

N

N

K

E

E

L

A

V

S

P

S

S

Y

Y

Q

E

E

E

L

R

I

I

E

K

-

A

-

N

-

S

-

P

G

A

Y

Y

L

L

D

D

R

Y

P

T

T

P

V

A

K

K

K

Q

E

-

M

-

T

L

L

I

E

E

R

K

E

E

C12 (= C16) binding [4Fe-4S] cluster
C15 (= C19) binding [4Fe-4S] cluster
C46 (= C50) binding [4Fe-4S] cluster
K49 (≠ R53) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D92) binding Mg(2+)
GD 91:92 (= GD 93:94) binding thiamine diphosphate
N118 (= N120) binding Mg(2+)
V120 (≠ I122) binding Mg(2+)
GL 122:123 (= GL 124:125) binding thiamine diphosphate
K125 (= K127) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (= K175) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C199) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
38% identity, 97% coverage: 1:278/288 of query aligns to 1:288/305 of P72579

query
sites
P72579

M

M

A

A

T

A

F

F

K

T

D

P

F

Q

R

W

N

N

N

D

I

-

K

-

P

-

N

-

W

W

C

C

P

P

G

G

C

C

G

G

D

N

F

F

S

G

V

I

Q

L

A

N

A

A

I

E

Q

Q

K

Q

A

A

C

I

A

V

A

E

N

L

G

G

L

V

E

D

P

T

D

K

E

N

L

V

A

V

V

V

I

V

T

S

G

G

I

I

G

G

C

C

S

S

G

G

R
x
K

L

I

S

P

G

H

Y

F

I

F

K

R

S

T

-

P

-

I

Y

S

G

G

F

V

H

H

A

T

T

L

H

H

G

G

R

R

S

A

L

I

P

A

V

F

A

A

Q

T

G

G

V

I

K

K

M

L

A

S

N

N

D

P

E

D

L

L

T

V

V

V

I

I

A

V

S

N

G

G

D

D

G

G

D

D

G

L

F

L

A

G

I

I

G

G

M

A

G

G

H

H

T

F

V

V

H

A

A

A

L

G

K

R

R

R

N

N

I

V

D

D

I

M

T

V

Y

V

I

I

V

L

M

H

D

D

N

N

Q

G

I

V

Y

Y

G

G

L
|
L

T

T

K

K

G

G

Q

Q

T

A

S

S

P

P

S

T

S

L

E

K

K

R

G

G

F

E

V

K

T

P

K

K

S

S

T

L

P

P

N

R

G

P

S

N

I

I

E

N

N

D

A

A

I

V

Q

N

P

P

I

I

R

A

T

L

A

A

M

I

A

S

S

S

G

G

A

Y

T

T

F

F

I

V

A

A

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R

S

G

V

Y

S

A

S

Y

D

D

I

V

K

K

Q

H

L

L

T

K

A

E

I

L

I

I

Q

K

E

S

A

A

M

I

N

K

H

H

K

K

G

G

F

L

S

A

F

L

V

I

N

D

V

V

F

L

S

Q

P

P

C

C

V

P

T

T

Y

Y

N

N

K

D

V

I

N

N

T

T

Y

K

D

E

W

W

F

Y

K

D

E

K

N

R

L

I

T

Y

A

K

V

L

E

D

D

T

I

L

G

P

D

D

Y

W

D

D

-

P

-

V

-

K

-

P

-

E

-

V

N

N

T

E

D

K

Q

I

K

K

A

R

A

A

M

I

N

D

H

K

I

S

M

L

E

E

H

W

-

G

E

D

G

R

L

I

L

P

K

I

G

G

I

I

I

F

Y

Y

Q

Q

N

N

K

E

E

L

A

V

S

P

S

S

Y

Y

Q

E

E

E

L

R

I

I

E

K

-

A

-

N

-

S

-

P

G

A

Y

Y

L

L

D

D

R

Y

P

T

T

P

V

A

K

K

K

Q

E

-

M

-

T

L

L

I

E

E

R

K

E

E

K49 (≠ R53) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L125) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
38% identity, 92% coverage: 14:278/288 of query aligns to 6:270/286 of 5b48B

query
sites
5b48B

N

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

N

F

F

S

G

V

I

Q

L

A

N

A

A

I

E

Q

Q

K

Q

A

A

C

I

A

V

A

E

N

L

G

G

L

V

E

D

P

T

D

K

E

N

L

V

A

V

V

V

I

V

T

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

G

G

R

K

L

I

S

P

G

H

Y

F

I

T

K

P

S

I

Y

S

G

G

F

V

H

H

A

T

T

L

H
|
H

G

G

R

R

S

A

L

I

P

A

V

F

A

A

Q

T

G

G

V

I

K

K

M

L

A

S

N

N

D

P

E

D

L

L

T

V

V

V

I

I

A

V

S

N

G

G

D
|
D

G
|
G

D
|
D

G

L

F

L

A

G

I

I

G

G

M

A

G

G

H

H

T

F

V

V

H

A

A

A

L

G

K

R

R

R

N

N

I

V

D

D

I

M

T

V

Y

V

I

I

V

L

M

H

D

D

N
|
N

Q

G

I
x
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

T

A

S

S

P

P

S

T

S

L

E

K

K

R

G

G

F

-

V

-

T

-

K

-

S

-

T

-

P

-

N

-

G

E

S

N

I

I

E

N

N

D

A

A

I

V

Q

N

P

P

I

I

R

A

T

L

A

A

M

I

A

S

S

S

G

G

A

Y

T

T

F

F

I

V

A

A

Q

R

S

G

V

Y

S

A

S

Y

D

D

I

V

K

K

Q

H

L

L

T

K

A

E

I

L

I

I

Q

K

E

S

A

A

M

I

N

K

H

H

K

K

G

G

F

L

S

A

F

L

V

I

N

D

V

V

F

L

S

Q

P

P

C
|
C

V

P

T
|
T

Y

Y

N

N

K

D

V

I

N

N

T

T

Y

K

D

E

W

W

F

-

K

-

E

-

N

R

L

I

T

Y

A

K

V

L

E

D

D

T

I

L

G

P

D

D

Y

W

D

D

-

P

-

V

-

K

-

P

-

E

-

V

N

N

T

E

D

K

Q

I

K

K

A

R

A

A

M

I

N

D

H

K

I

S

M

L

E

E

H

W

E

G

G

D

L

I

L

P

K

I

G

G

I

I

I

F

Y

Y

Q

Q

N

N

K

E

E

L

A

V

S

P

S

S

Y

Y

Q

E

E

E

L

R

I

I

E

K

-

A

-

N

-

S

-

P

G

A

Y

Y

L

L

D

D

R

Y

P

T

T

P

V

A

K

K

K

Q

E

-

M

-

T

L

L

I

E

E

R

K

E

E

binding magnesium ion: D84 (= D92), N112 (= N120), V114 (≠ I122)
binding iron/sulfur cluster: C8 (= C16), C11 (= C19), C42 (= C50), C183 (= C199), T185 (= T201)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I48), G41 (= G49), C42 (= C50), S43 (= S51), H59 (= H67), G85 (= G93), D86 (= D94), N112 (= N120), V114 (≠ I122), Y115 (= Y123), G116 (= G124), L117 (= L125)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
36% identity, 85% coverage: 14:258/288 of query aligns to 3:237/275 of 5b47B

query
sites
5b47B

N

D

W

W

C
|
C

P

P

G

G

C
|
C

G

G

D

D

F

F

S

G

V

I

Q

L

A

R

A

A

I

E

Q

E

K

M

A

A

C

I

A

R

A

E

N

L

G

G

L

I

E

N

P

P

D

K

E

S

L

V

A

V

V

I

I

V

T

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

G

G

R

K

L

I

S

P

G

H

Y

F

-

M

-

N

I

L

K

P

S

I

Y

S

G

G

F

V

H

H

A

T

T

L

H
|
H

G

G

R

R

S

S

L

I

P

A

V

F

A

A

Q

T

G

G

V

I

K

K

M

L

A

S

N

N

D

P

E

S

L

L

T

E

V

V

I

I

A

V

S

N

G

V

G

G

D
|
D

G
|
G

D
|
D

G

G

F

L

A

G

I

I

G

G

M

M

G

G

H

H

T

F

V

V

H

H

A

L

L

G

K

R

R

R

N

N

I

I

D

D

I

I

T

A

Y

V

I

L

V

V

M

H

D

N

N
|
N

Q

G

I
x
V

Y
|
Y

G

G

L
|
L

T

-

K

-

G

-

Q

Q

T

A

S

S

P

P

S

T

S

L

E

H

K

R

G

G

F

-

V

-

T

-

K

-

S

-

T

-

P

-

N

-

G

E

S

N

I

I

E

M

N

D

A

A

I

V

Q

N

P

P

I

L

R

A

T

V

A

A

M

L

A

A

S

A

G

G

A

Y

T

T

F

F

I

V

A

A

Q

R

S

G

V

Y

S

A

S

Y

D

D

I

V

K

M

Q

H

L

L

T

K

A

E

I

L

I

I

Q

K

E

K

A

A

M

I

N

L

H

H

K

K

G

G

F

S

S

A

F

L

V

V

N

D

V

I

F

L

S

Q

P

P

C
|
C

V

P

T

T

Y

Y

N

-

K

R

V

V

N

Y

T

K

Y

L

D

D

W

-

F

-

K

-

E

-

N

N

L

V

T

P

A

G

V

W

E

D

D

P

I

V

G

V

D

R

Y

K

D

E

N

E

T

E

D

A

Q

Q

K

K

A

K

A

F

M

E

N

Q

H

A

I

I

M

M

E

K

H

S

-

Y

-

E

-

W

-

G

E

E

G

K

L

I

L

P

K

I

G

G

I

I

I

F

Y

Y

Q

Q

N

N

K

E

E

L

A

V

S

P

S

T

Y

F

Q

E

E

D

binding magnesium ion: D83 (= D92), N111 (= N120)
binding iron/sulfur cluster: C5 (= C16), C8 (= C19), C39 (= C50), C179 (= C199)
binding thiamine diphosphate: I37 (= I48), G38 (= G49), C39 (= C50), S40 (= S51), H58 (= H67), G84 (= G93), D85 (= D94), N111 (= N120), V113 (≠ I122), Y114 (= Y123), L116 (= L125)

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
28% identity, 45% coverage: 90:219/288 of query aligns to 964:1093/1165 of 6cipA

query
sites
6cipA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

A

D

N

I

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

Q

E

I
x
V

Y

Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

Q

K

T

G

G

F

A

V

V

T

A

K

R

S

F

T

A

P

A

N

G

G

G

S

K

I

F

E

T

N

K

A

K

I

K

Q

D

P

L

I

G

R

L

T

M

A

A

M

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

S

-

V

S

A

V

M

S

G

S

A

D

S

I

H

K

S

Q

Q

L

L

T

M

A

K

I

A

I

L

Q

I

E

E

A

A

M

E

N

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

F

Y

S

A

P

P

C
|
C

V

I

T

N

Y

H

N

G

K

I

V

N

N

M

T

T

Y

Y

D

S

W

Q

F

-

K

R

E

E

N

A

L

K

T

K

A

A

V

V

E

E

active site: N999 (≠ L125)
binding 2-acetyl-thiamine diphosphate: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), S998 (≠ G124), N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: S998 (≠ G124), C1074 (= C199)

Sites not aligning to the query:

active site: 28, 61, 111
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
28% identity, 45% coverage: 90:219/288 of query aligns to 965:1094/1171 of Q2RMD6

query
sites
Q2RMD6

G

G

D
|
D

G
|
G

D
x
W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

A

D

N

I

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

Q
x
E

I
x
V

Y
|
Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

Q

K

T

G

G

F

A

V

V

T

A

K

R

S

F

T

A

P

A

N

G

G

G

S

K

I

F

E

T

N

K

A

K

I

K

Q

D

P

L

I

G

R

L

T

M

A

A

M

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

S

-

V

S

A

V

M

S

G

S

A

D

S

I

H

K

S

Q

Q

L

L

T

M

A

K

I

A

I

L

Q

I

E

E

A

A

M

E

N

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

F

Y

S

A

P

P

C
|
C

V

I

T

N

Y

H

N

G

K

I

V

N

N

M

T

T

Y

Y

D

S

W

Q

F

-

K

R

E

E

N

A

L

K

T

K

A

A

V

V

E

E

D967 (= D92) binding Mg(2+)
DGW 967:969 (≠ DGD 92:94) binding thiamine diphosphate
T995 (≠ N120) binding Mg(2+)
TEVYSN 995:1000 (≠ NQIYGL 120:125) binding thiamine diphosphate
V997 (≠ I122) binding Mg(2+)
C1075 (= C199) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
28% identity, 45% coverage: 90:219/288 of query aligns to 964:1093/1169 of 6ciqA

query
sites
6ciqA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

A

D

N

I

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

Q

K

T

G

G

F

A

V

V

T

A

K

R

S

F

T

A

P

A

N

G

G

G

S

K

I

F

E

T

N

K

A

K

I

K

Q

D

P

L

I

G

R

L

T

M

A

A

M

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

S

-

V

S

A

V

M

S

G

S

A

D

S

I

H

K

S

Q

Q

L

L

T

M

A

K

I

A

I

L

Q

I

E

E

A

A

M

E

N

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

F

Y

S

A

P

P

C
|
C

V
x
I

T

N

Y

H

N

G

K

I

V

N

N

M

T

T

Y

Y

D

S

W

Q

F

-

K

R

E

E

N

A

L

K

T

K

A

A

V

V

E

E

active site: N999 (≠ L125)
binding coenzyme a: N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: S998 (≠ G124), C1074 (= C199), I1075 (≠ V200)
binding thiamine diphosphate: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), Y997 (= Y123), S998 (≠ G124), N999 (≠ L125), T1000 (= T126)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
28% identity, 45% coverage: 90:219/288 of query aligns to 964:1093/1169 of 6cinB

query
sites
6cinB

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

A

D

N

I

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

Q

K

T

G

G

F

A

V

V

T

A

K

R

S

F

T

A

P

A

N

G

G

G

S

K

I

F

E

T

N

K

A

K

I

K

Q

D

P

L

I

G

R

L

T

M

A

A

M

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

S

-

V

S

A

V

M

S

G

S

A

D

S

I

H

K

S

Q

Q

L

L

T

M

A

K

I

A

I

L

Q

I

E

E

A

A

M

E

N

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

F

Y

S

A

P

P

C
|
C

V
x
I

T

N

Y

H

N

G

K

I

V

N

N

M

T

T

Y

Y

D

S

W

Q

F

-

K

R

E

E

N

A

L

K

T

K

A

A

V

V

E

E

active site: N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: S998 (≠ G124), C1074 (= C199), I1075 (≠ V200)
binding thiamine diphosphate: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), Y997 (= Y123), S998 (≠ G124), N999 (≠ L125), T1000 (= T126)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
28% identity, 45% coverage: 90:219/288 of query aligns to 964:1093/1164 of 6cioA

query
sites
6cioA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

A

D

N

I

V

T

N

Y

V

I

L

V

V

M

L

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

Q

K

T

G

G

F

A

V

V

T

A

K

R

S

F

T

A

P

A

N

G

G

G

S

K

I

F

E

T

N

K

A

K

I

K

Q

D

P

L

I

G

R

L

T

M

A

A

M

M

A

S

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

S

-

V

S

A

V

M

S

G

S

A

D

S

I

H

K

S

Q

Q

L

L

T

M

A

K

I

A

I

L

Q

I

E

E

A

A

M

E

N

K

H

Y

K

D

G

G

F

P

S

S

F

L

V

I

N

I

V

A

F

Y

S

A

P

P

C
|
C

V
x
I

T

N

Y

H

N

G

K

I

V

N

N

M

T

T

Y

Y

D

S

W

Q

F

-

K

R

E

E

N

A

L

K

T

K

A

A

V

V

E

E

active site: N999 (≠ L125)
binding magnesium ion: D966 (= D92), T994 (≠ N120), V996 (≠ I122)
binding iron/sulfur cluster: C1074 (= C199), I1075 (≠ V200)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G965 (= G91), D966 (= D92), G967 (= G93), V996 (≠ I122), Y997 (= Y123), S998 (≠ G124), N999 (≠ L125), T1000 (= T126)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865

7plmA Cryoem reconstruction of pyruvate ferredoxin oxidoreductase (pfor) in anaerobic conditions (see paper)
30% identity, 39% coverage: 90:200/288 of query aligns to 928:1039/1177 of 7plmA

query
sites
7plmA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

E

D
|
D

I

V

T
x
N

Y

V

I

F

V

V

M

M

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

P

K

T

G

G

F

A

V

V

T

A

K

K

S

F

T

A

P

A

N

A

G

G

S

K

I

R

E

T

N

G

A

K

I

K

Q

D

P

L

I

A

R

R

T

M

A

V

M

M

A

T

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

T

-

V

S

S

V

M

S

G

S

Y

D

S

I

K

K

Q

Q

Q

L

F

T

L

A

K

I

V

I

L

Q

K

E

E

A

A

M

E

N

S

H
x
F

K

P

G
|
G

F

P

S

S

F

L

V

V

N

I

V

A

F

Y

S

A

P

T

C
|
C

V
x
I

binding calcium ion: D950 (= D112), N952 (≠ T114), F1026 (≠ H187), G1028 (= G189)
binding magnesium ion: D930 (= D92), T958 (≠ N120), V960 (≠ I122), S962 (≠ G124)
binding iron/sulfur cluster: S962 (≠ G124), C1038 (= C199), I1039 (≠ V200)
binding thiamine diphosphate: G929 (= G91), D930 (= D92), G931 (= G93), V960 (≠ I122), Y961 (= Y123), S962 (≠ G124), N963 (≠ L125), T964 (= T126)

Sites not aligning to the query:

binding iron/sulfur cluster: 458, 657, 659, 664, 665, 666, 667, 668, 670, 674, 675, 679, 708, 715, 716, 717, 718, 719, 721, 725, 726, 731, 732, 781, 782, 785, 787, 810
binding thiamine diphosphate: 27, 28, 29, 63, 787, 808, 809, 810, 839

2c3uA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus, oxygen inhibited form (see paper)
30% identity, 39% coverage: 90:200/288 of query aligns to 960:1071/1231 of 2c3uA

query
sites
2c3uA

G

G

G
|
G

D
|
D

G
|
G

D
x
W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

E

D
|
D

I

V

T
x
N

Y

V

I

F

V

V

M

M

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

P

K

T

G

G

F

A

V

V

T

A

K

K

S

F

T

A

P

A

N

A

G

G

S

K

I

R

E

T

N

G

A

K

I

K

Q

D

P

L

I

A

R

R

T

M

A

V

M

M

A

T

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

T

-

V

S

S

V

M

S

G

S

Y

D

S

I

K

K

Q

Q

Q

L

F

T

L

A

K

I

V

I

L

Q

K

E

E

A
|
A

M
x
E

N

S

H
x
F

K

P

G
|
G

F

P

S
|
S

F

L

V

V

N

I

V

A

F

Y

S

A

P

T

C
|
C

V
x
I

active site: N995 (≠ L125)
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: G961 (= G91), D962 (= D92), G963 (= G93), W964 (≠ D94), V992 (≠ I122), Y993 (= Y123), S994 (≠ G124), N995 (≠ L125), T996 (= T126)
binding calcium ion: D982 (= D112), N984 (≠ T114), A1055 (= A184), E1056 (≠ M185), F1058 (≠ H187), G1060 (= G189), S1062 (= S191)
binding magnesium ion: D962 (= D92), T990 (≠ N120), V992 (≠ I122)
binding pyruvic acid: N995 (≠ L125)
binding iron/sulfur cluster: C1070 (= C199), I1071 (≠ V200)

Sites not aligning to the query:

active site: 30, 63, 113
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: 28, 29, 63, 87, 816, 837, 839, 868, 869
binding pyruvic acid: 30, 113, 122
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 811, 814, 816, 839

2c3pA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
30% identity, 39% coverage: 90:200/288 of query aligns to 960:1071/1231 of 2c3pA

query
sites
2c3pA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

E

D
|
D

I
x
V

T
x
N

Y

V

I

F

V

V

M

M

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

P

K

T

G

G

F

A

V

V

T

A

K

K

S

F

T

A

P

A

N

A

G

G

S

K

I

R

E

T

N

G

A

K

I

K

Q

D

P

L

I

A

R

R

T

M

A

V

M

M

A

T

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

T

-

V

S

S

V

M

S

G

S

Y

D

S

I

K

K

Q

Q

Q

L

F

T

L

A

K

I

V

I

L

Q

K

E

E

A
|
A

M
x
E

N

S

H
x
F

K

P

G
|
G

F

P

S
|
S

F

L

V

V

N

I

V

A

F

Y

S

A

P

T

C
|
C

V
x
I

active site: N995 (≠ L125)
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: G961 (= G91), D962 (= D92), G963 (= G93), V992 (≠ I122), Y993 (= Y123), S994 (≠ G124), N995 (≠ L125), T996 (= T126)
binding calcium ion: D982 (= D112), V983 (≠ I113), N984 (≠ T114), A1055 (= A184), E1056 (≠ M185), F1058 (≠ H187), G1060 (= G189), S1062 (= S191)
binding magnesium ion: D962 (= D92), T990 (≠ N120), V992 (≠ I122)
binding iron/sulfur cluster: S994 (≠ G124), C1070 (= C199), I1071 (≠ V200)

Sites not aligning to the query:

active site: 30, 63, 113
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: 28, 29, 30, 63, 87, 113, 816, 837, 838, 839, 868, 869
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839

2c3oA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
30% identity, 39% coverage: 90:200/288 of query aligns to 960:1071/1231 of 2c3oA

query
sites
2c3oA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

E

D
|
D

I

V

T
x
N

Y

V

I

F

V

V

M

M

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

P

K

T

G

G

F

A

V

V

T

A

K

K

S

F

T

A

P

A

N

A

G

G

S

K

I

R

E

T

N

G

A

K

I

K

Q

D

P

L

I

A

R

R

T

M

A

V

M

M

A

T

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

T

-

V

S

S

V

M

S

G

S

Y

D

S

I

K

K

Q

Q

Q

L

F

T

L

A

K

I

V

I

L

Q

K

E

E

A

A

M
x
E

N

S

H
x
F

K

P

G
|
G

F

P

S

S

F

L

V

V

N

I

V

A

F

Y

S

A

P

T

C
|
C

V
x
I

active site: N995 (≠ L125)
binding calcium ion: D982 (= D112), N984 (≠ T114), E1056 (≠ M185), F1058 (≠ H187), G1060 (= G189)
binding magnesium ion: D962 (= D92), T990 (≠ N120), V992 (≠ I122)
binding pyruvic acid: T996 (= T126)
binding iron/sulfur cluster: C1070 (= C199), I1071 (≠ V200)
binding thiamine diphosphate: G961 (= G91), D962 (= D92), G963 (= G93), V992 (≠ I122), Y993 (= Y123), S994 (≠ G124), N995 (≠ L125), T996 (= T126)

Sites not aligning to the query:

active site: 30, 63, 113
binding pyruvic acid: 30, 113
binding iron/sulfur cluster: 458, 681, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 746, 747, 748, 749, 750, 754, 760, 761, 810, 811, 814, 816, 839
binding thiamine diphosphate: 28, 29, 63, 87, 816, 837, 838, 839, 868

1kekA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase (see paper)
30% identity, 39% coverage: 90:200/288 of query aligns to 960:1071/1231 of 1kekA

query
sites
1kekA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

E

D
|
D

I

V

T
x
N

Y

V

I

F

V

V

M

M

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

P

K

T

G

G

F

A

V

V

T

A

K

K

S

F

T

A

P

A

N

A

G

G

S

K

I

R

E

T

N

G

A

K

I

K

Q

D

P

L

I

A

R

R

T

M

A

V

M

M

A

T

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

T

-

V

S

S

V

M

S

G

S

Y

D

S

I

K

K

Q

Q

Q

L

F

T

L

A

K

I

V

I

L

Q

K

E

E

A
|
A

M
x
E

N

S

H
x
F

K

P

G

G

F

P

S
|
S

F

L

V

V

N

I

V

A

F

Y

S

A

P

T

C
|
C

V
x
I

active site: N995 (≠ L125)
binding calcium ion: D982 (= D112), N984 (≠ T114), A1055 (= A184), E1056 (≠ M185), F1058 (≠ H187), S1062 (= S191)
binding 2-acetyl-thiamine diphosphate: G961 (= G91), D962 (= D92), G963 (= G93), V992 (≠ I122), Y993 (= Y123), S994 (≠ G124), N995 (≠ L125), T996 (= T126)
binding magnesium ion: D962 (= D92), T990 (≠ N120), V992 (≠ I122)
binding iron/sulfur cluster: C1070 (= C199), I1071 (≠ V200)

Sites not aligning to the query:

active site: 30, 63, 113
binding 2-acetyl-thiamine diphosphate: 29, 63, 87, 113, 816, 837, 838, 839, 868
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 703, 737, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839

1b0pA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
30% identity, 39% coverage: 90:200/288 of query aligns to 960:1071/1231 of 1b0pA

query
sites
1b0pA

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

F

Y

A

D

I

I

G

G

M

Y

G

G

H

G

T

L

V

D

H

H

A

V

L

L

K

A

R

S

N

G

I

E

D
|
D

I

V

T
x
N

Y

V

I

F

V

V

M

M

D

D

N
x
T

Q

E

I
x
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

T

S

S

S

P

K

S

A

S

T

E

P

K

T

G

G

F

A

V

V

T

A

K

K

S

F

T

A

P

A

N

A

G

G

S

K

I

R

E

T

N

G

A

K

I

K

Q

D

P

L

I

A

R

R

T

M

A

V

M

M

A

T

S

Y

G

G

A

Y

T

V

F

Y

I

V

A

A

Q

T

-

V

S

S

V

M

S

G

S

Y

D

S

I

K

K

Q

Q

Q

L

F

T

L

A

K

I

V

I

L

Q

K

E

E

A
|
A

M
x
E

N

S

H
x
F

K

P

G
|
G

F

P

S

S

F

L

V

V

N

I

V

A

F

Y

S

A

P

T

C
|
C

V
x
I

active site: N995 (≠ L125)
binding calcium ion: D982 (= D112), N984 (≠ T114), A1055 (= A184), E1056 (≠ M185), F1058 (≠ H187), G1060 (= G189)
binding magnesium ion: D962 (= D92), T990 (≠ N120), V992 (≠ I122)
binding iron/sulfur cluster: S994 (≠ G124), C1070 (= C199), I1071 (≠ V200)
binding thiamine diphosphate: G961 (= G91), D962 (= D92), G963 (= G93), V992 (≠ I122), Y993 (= Y123), S994 (≠ G124), N995 (≠ L125), T996 (= T126)

Sites not aligning to the query:

active site: 30, 63, 113
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 737, 744, 745, 747, 748, 750, 754, 755, 760, 811, 814, 816, 839
binding thiamine diphosphate: 28, 29, 63, 816, 837, 838, 839, 868

Query Sequence

>WP_017548568.1 NCBI__GCF_000330705.1:WP_017548568.1
MATFKDFRNNIKPNWCPGCGDFSVQAAIQKACAANGLEPDELAVITGIGCSGRLSGYIKS
YGFHATHGRSLPVAQGVKMANDELTVIASGGDGDGFAIGMGHTVHALKRNIDITYIVMDN
QIYGLTKGQTSPSSEKGFVTKSTPNGSIENAIQPIRTAMASGATFIAQSVSSDIKQLTAI
IQEAMNHKGFSFVNVFSPCVTYNKVNTYDWFKENLTAVEDIGDYDNTDQKAAMNHIMEHE
GLLKGIIYQNKEASSYQELIEGYLDRPTVKKEMTLERETFDDLMAAFR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory