SitesBLAST
Comparing WP_017548686.1 NCBI__GCF_000330705.1:WP_017548686.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1nfqA Rv2002 gene product from mycobacterium tuberculosis (see paper)
47% identity, 99% coverage: 2:242/243 of query aligns to 2:239/244 of 1nfqA
- active site: G17 (= G17), S139 (= S139), Y152 (= Y152), K156 (= K156)
- binding Androsterone: L91 (≠ N91), E141 (≠ G141), C149 (≠ S149), Y152 (= Y152), V193 (= V199), I197 (vs. gap), F198 (vs. gap)
- binding 1,4-dihydronicotinamide adenine dinucleotide: R16 (≠ G16), G17 (= G17), M18 (= M18), D37 (= D37), L39 (≠ D39), L59 (≠ Q59), D60 (= D60), V61 (= V61), N87 (= N87), A88 (= A88), I137 (= I137), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), V185 (= V185), T187 (= T187), P188 (≠ G188), M189 (≠ L189), T190 (≠ F190)
1nffA Crystal structure of rv2002 gene product from mycobacterium tuberculosis (see paper)
47% identity, 99% coverage: 2:242/243 of query aligns to 2:239/244 of 1nffA
- active site: G17 (= G17), S139 (= S139), Y152 (= Y152), K156 (= K156)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), R16 (≠ G16), G17 (= G17), M18 (= M18), D37 (= D37), I38 (≠ L38), L39 (≠ D39), L59 (≠ Q59), D60 (= D60), V61 (= V61), N87 (= N87), A88 (= A88), G89 (= G89), I90 (≠ V90), I137 (= I137), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), V185 (= V185), T187 (= T187), P188 (≠ G188), M189 (≠ L189), T190 (≠ F190)
P9WGT1 3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase; NADH-dependent 3alpha, 20beta-hydroxysteroid dehydrogenase; EC 1.1.1.53 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
47% identity, 99% coverage: 2:242/243 of query aligns to 3:240/260 of P9WGT1
- I6 (≠ N5) mutation to T: Maximal improvement in solubility; when associated with M-47 and K-69.
- RGM 17:19 (≠ GGM 16:18) binding NAD(+)
- D38 (= D37) binding NAD(+)
- V47 (≠ L46) mutation to M: Maximal improvement in solubility; when associated with T-6 and K-69.
- DV 61:62 (= DV 60:61) binding NAD(+)
- T69 (≠ N68) mutation to K: Maximal improvement in solubility; when associated with T-6 and M-47.
- N88 (= N87) binding NAD(+)
- S140 (= S139) mutation to A: Complete loss of both oxidation of androsterone and reduction of progesterone; when associated with T6; M-47 and K-69.
- Y153 (= Y152) binding NAD(+); mutation to F: Complete loss of both oxidation of androsterone and reduction of progesterone; when associated with T6; M-47 and K-69.
- K157 (= K156) binding NAD(+)
- 183:191 (vs. 182:190, 44% identical) binding NAD(+)
7ejhA Crystal structure of kred mutant-f147l/l153q/y190p/l199a/m205f/m206f and 2-hydroxyisoindoline-1,3-dione complex
41% identity, 99% coverage: 3:243/243 of query aligns to 5:253/253 of 7ejhA
- binding 2-oxidanylisoindole-1,3-dione: S144 (= S139), I145 (= I140), E146 (≠ G141), Y157 (= Y152), V197 (≠ F190), F207 (≠ A200)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), T17 (= T15), I20 (≠ M18), R40 (≠ L38), H41 (≠ D39), D64 (= D60), A65 (≠ V61), N91 (= N87), A92 (= A88), V114 (= V110), M142 (≠ I137), S144 (= S139), Y157 (= Y152), K161 (= K156), P189 (= P182), G190 (≠ S183), P191 (≠ L184), I192 (≠ V185), T194 (= T187), P195 (≠ G188), L196 (= L189)
7ejiB Crystal structure of kred f147l/l153q/y190p/l199a/m205f/m206f variant and methyl methacrylate complex
41% identity, 99% coverage: 3:243/243 of query aligns to 3:251/251 of 7ejiB
- binding methyl 2-methylprop-2-enoate: S142 (= S139), I143 (= I140), Y155 (= Y152), F205 (≠ A200)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (= T15), L16 (≠ G16), G17 (= G17), I18 (≠ M18), R38 (≠ L38), H39 (≠ D39), D62 (= D60), A63 (≠ V61), N89 (= N87), A90 (= A88), V112 (= V110), M140 (≠ I137), S142 (= S139), Y155 (= Y152), K159 (= K156), P187 (= P182), P189 (≠ L184), I190 (≠ V185), T192 (= T187), P193 (≠ G188), L194 (= L189)
1zk1A Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
41% identity, 99% coverage: 3:243/243 of query aligns to 3:251/251 of 1zk1A
- active site: G17 (= G17), S142 (= S139), Y155 (= Y152), K159 (= K156)
- binding 1-phenylethanone: A93 (≠ N91), N95 (≠ S93), Y155 (= Y152), Y189 (≠ L184)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ G16), I18 (≠ M18), D37 (= D37), H61 (≠ Q59), D62 (= D60), S63 (≠ V61), N89 (= N87), A90 (= A88), I92 (≠ V90), M140 (≠ I137), Y155 (= Y152), G188 (≠ S183), I190 (≠ V185), L194 (= L189)
1zjzA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and NAD (see paper)
41% identity, 99% coverage: 3:243/243 of query aligns to 3:251/251 of 1zjzA
- active site: G17 (= G17), S142 (= S139), Y155 (= Y152), K159 (= K156)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), L16 (≠ G16), I18 (≠ M18), D37 (= D37), D62 (= D60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ V90), Y155 (= Y152), G188 (≠ S183), I190 (≠ V185), L194 (= L189)
- binding (1r)-1-phenylethanol: A93 (≠ N91), N95 (≠ S93), L152 (≠ S149), Y155 (= Y152)
1zjyA Structure of r-specific alcohol dehydrogenase (mutant g37d) from lactobacillus brevis in complex with phenylethanol and nadh (see paper)
41% identity, 99% coverage: 3:243/243 of query aligns to 3:251/251 of 1zjyA
- active site: G17 (= G17), S142 (= S139), Y155 (= Y152), K159 (= K156)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G13 (= G13), L16 (≠ G16), G17 (= G17), I18 (≠ M18), D37 (= D37), D62 (= D60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ V90), Y155 (= Y152), G188 (≠ S183), I190 (≠ V185), L194 (= L189)
- binding (1r)-1-phenylethanol: A93 (≠ N91), N95 (≠ S93), L152 (≠ S149), Y155 (= Y152), Y189 (≠ L184)
Q6WVP7 NADP-dependent (R)-specific alcohol dehydrogenase; (R)-specific ADH; Ketoreductase; KRED; EC 1.1.1.- from Lentilactobacillus kefiri (Lactobacillus kefiri) (see paper)
41% identity, 99% coverage: 3:243/243 of query aligns to 4:252/252 of Q6WVP7
- TLGI 16:19 (≠ TGGM 15:18) binding NADP(+)
- RH 39:40 (≠ LD 38:39) binding NADP(+)
- DA 63:64 (≠ DV 60:61) binding NADP(+)
- N90 (= N87) binding NADP(+)
- Y156 (= Y152) binding NADP(+)
- K160 (= K156) binding NADP(+)
- IKTPL 191:195 (≠ VNTGL 185:189) binding NADP(+)
- Q252 (≠ R243) binding Mg(2+)
2hsdA The refined three-dimensional structure of 3alpha,20beta- hydroxysteroid dehydrogenase and possible roles of the residues conserved in short-chain dehydrogenases (see paper)
41% identity, 98% coverage: 4:241/243 of query aligns to 3:242/253 of 2hsdA
- active site: G16 (= G17), S138 (= S139), Y151 (= Y152), K155 (= K156)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), R15 (≠ G16), G16 (= G17), L17 (≠ M18), D36 (= D37), V37 (≠ L38), L58 (≠ Q59), V60 (= V61), N86 (= N87), A87 (= A88), S138 (= S139), Y151 (= Y152), K155 (= K156), P181 (= P182), G182 (≠ S183), T184 (≠ V185)
1hdcA Mechanism of inhibition of 3alpha,20beta-hydroxysteroid dehydrogenase by a licorice-derived steroidal inhibitor (see paper)
41% identity, 98% coverage: 4:241/243 of query aligns to 3:242/253 of 1hdcA
- active site: G16 (= G17), S138 (= S139), Y151 (= Y152), K155 (= K156)
- binding carbenoxolone: S90 (≠ N91), T91 (≠ Y92), G92 (≠ S93), L147 (≠ E148), Y151 (= Y152), M183 (≠ L184), M188 (≠ L189), T189 (≠ F190), T192 (≠ S193)
6y0sAAA R-specific alcohol dehydrogenase (see paper)
40% identity, 99% coverage: 3:243/243 of query aligns to 3:251/251 of 6y0sAAA
1zk4A Structure of r-specific alcohol dehydrogenase (wildtype) from lactobacillus brevis in complex with acetophenone and NADP (see paper)
40% identity, 99% coverage: 3:243/243 of query aligns to 3:251/251 of 1zk4A
- active site: G17 (= G17), S142 (= S139), Y155 (= Y152), K159 (= K156)
- binding 1-phenylethanone: A93 (≠ N91), Y155 (= Y152), Y189 (≠ L184)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G13), T15 (= T15), L16 (≠ G16), I18 (≠ M18), T36 (= T36), G37 (≠ D37), R38 (≠ L38), H61 (≠ Q59), D62 (= D60), N89 (= N87), A90 (= A88), G91 (= G89), I92 (≠ V90), Y155 (= Y152), G188 (≠ S183), I190 (≠ V185), T192 (= T187), L194 (= L189)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
36% identity, 99% coverage: 1:240/243 of query aligns to 2:242/244 of 4nbuB
- active site: G18 (= G17), N111 (= N111), S139 (= S139), Q149 (≠ S149), Y152 (= Y152), K156 (= K156)
- binding acetoacetyl-coenzyme a: D93 (≠ S93), K98 (≠ A98), S139 (= S139), N146 (≠ R146), V147 (≠ M147), Q149 (≠ S149), Y152 (= Y152), F184 (≠ L184), M189 (≠ L189), K200 (≠ A198)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ G16), G18 (= G17), I19 (≠ M18), D38 (= D37), F39 (≠ L38), V59 (≠ Q59), D60 (= D60), V61 (= V61), N87 (= N87), A88 (= A88), G89 (= G89), I90 (≠ V90), T137 (≠ I137), S139 (= S139), Y152 (= Y152), K156 (= K156), P182 (= P182), F184 (≠ L184), T185 (≠ V185), T187 (= T187), M189 (≠ L189)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
35% identity, 99% coverage: 4:243/243 of query aligns to 4:260/260 of 6zzqA
- active site: G17 (= G17), S142 (= S139), Y155 (= Y152)
- binding acetoacetic acid: Q94 (≠ N91), S142 (= S139), K152 (≠ S149), Y155 (= Y152), Q196 (≠ L189)
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), S16 (≠ G16), G17 (= G17), I18 (≠ M18), D37 (= D37), M38 (≠ L38), D63 (= D60), V64 (= V61), N90 (= N87), A91 (= A88), G92 (= G89), M140 (≠ I137), A141 (≠ S138), S142 (= S139), Y155 (= Y152), K159 (= K156), Y187 (≠ L184), V188 (= V185), T190 (= T187)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
35% identity, 99% coverage: 4:243/243 of query aligns to 5:261/261 of 6zzsD
- active site: G18 (= G17), S143 (= S139), Y156 (= Y152)
- binding nicotinamide-adenine-dinucleotide: G14 (= G13), S17 (≠ G16), I19 (≠ M18), D38 (= D37), M39 (≠ L38), D64 (= D60), V65 (= V61), N91 (= N87), A92 (= A88), G93 (= G89), M141 (≠ I137), A142 (≠ S138), S143 (= S139), Y156 (= Y152), K160 (= K156), P186 (= P182), G187 (≠ S183), V189 (= V185), T191 (= T187), L193 (vs. gap)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ N91), S143 (= S139), N145 (≠ G141), K153 (≠ S149), Y156 (= Y152), Q197 (≠ L189)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
34% identity, 99% coverage: 3:242/243 of query aligns to 7:252/258 of 4wecA