SitesBLAST

7lnuB Ternary complex of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus bound to isopentenyl monophosphate and atp (see paper)
28% identity, 48% coverage: 126:258/277 of query aligns to 123:250/261 of 7lnuB

query
sites
7lnuB

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binding adenosine-5'-diphosphate: S172 (= S178), D173 (= D179), I174 (≠ V180), Y178 (= Y184), A180 (≠ S186), K183 (≠ H189), K218 (= K226)
binding Isopentenyl phosphate: I149 (≠ T153), S151 (≠ F155), G152 (= G156)

Sites not aligning to the query:

7n9dA I74a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
27% identity, 48% coverage: 126:258/277 of query aligns to 121:246/253 of 7n9dA

query
sites
7n9dA

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binding (2E)-3-phenylbut-2-en-1-yl dihydrogen phosphate: G135 (≠ N141), D136 (= D142), V137 (≠ A143), I147 (≠ T153), S149 (≠ F155), G150 (= G156), T208 (= T220)
binding adenosine-5'-diphosphate: S170 (= S178), D171 (= D179), I172 (≠ V180), Y176 (= Y184), A178 (≠ S186), D179 (≠ N187), D201 (≠ G213), G210 (= G222), V211 (≠ M223), K214 (= K226)

Sites not aligning to the query:

binding (2E)-3-phenylbut-2-en-1-yl dihydrogen phosphate: 45, 46, 50, 51, 75, 79
binding adenosine-5'-diphosphate: 8, 9, 10, 15

7lnwA I146a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
26% identity, 48% coverage: 126:258/277 of query aligns to 121:245/256 of 7lnwA

query
sites
7lnwA

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binding adenosine-5'-diphosphate: D171 (= D179), I172 (≠ V180), Y176 (= Y184), D179 (≠ N187), P180 (= P188), K181 (≠ H189), V210 (≠ M223), K213 (= K226)
binding [(~{Z})-5-[(azanylidene-$l^{4}-azanylidene)amino]-3-methyl-pent-2-enyl] dihydrogen phosphate: G135 (≠ N141), D136 (= D142), V148 (≠ K154), S149 (≠ F155)

Sites not aligning to the query:

7lnxB I146a mutant of the isopentenyl phosphate kinase from candidatus methanomethylophilus alvus (see paper)
25% identity, 48% coverage: 126:258/277 of query aligns to 125:236/247 of 7lnxB

query
sites
7lnxB

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binding adenosine-5'-diphosphate: S174 (= S178), D175 (= D179), I176 (≠ V180), Y180 (= Y184), A182 (≠ S186), D183 (≠ N187), P184 (= P188), K185 (≠ H189), K204 (= K226)
binding (2E)-3-methylhept-2-en-1-yl dihydrogen phosphate: D140 (= D142), V141 (≠ A143), A151 (≠ T153), G154 (= G156)

Sites not aligning to the query:

2bmuB Ump kinase from pyrococcus furiosus complexed with its substrate ump and its substrate analog amppnp (see paper)
29% identity, 41% coverage: 150:262/277 of query aligns to 118:222/226 of 2bmuB

query
sites
2bmuB

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binding phosphoaminophosphonic acid-adenylate ester: T141 (≠ S178), N142 (≠ D179), V146 (≠ L183), Y147 (= Y184), A149 (≠ S186), D150 (≠ N187), P151 (= P188), S182 (≠ G215), S183 (= S216), V184 (= V217)
binding magnesium ion: D122 (= D159), D122 (= D159), S183 (= S216), V184 (= V217)
binding uridine-5'-monophosphate: H119 (= H151), T120 (≠ C152), T121 (= T153), A179 (vs. gap)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 9, 10, 44, 45, 46
binding magnesium ion: 7
binding uridine-5'-monophosphate: 44, 45, 67, 70, 71, 114, 115, 116

Q8U122 Uridylate kinase; UK; Uridine monophosphate kinase; UMP kinase; UMPK; EC 2.7.4.22 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see paper)
29% identity, 41% coverage: 150:262/277 of query aligns to 117:221/225 of Q8U122

query
sites
Q8U122

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T120 (= T153) binding Mg(2+)
D121 (= D159) binding Mg(2+); binding Mg(2+)
G179 (= G213) binding UMP
S182 (= S216) binding Mg(2+)

Sites not aligning to the query:

6 binding Mg(2+)
44 binding UMP
66 binding UMP
114:120 binding UMP

2ji5A Structure of ump kinase from pyrococcus furiosus complexed with utp
33% identity, 28% coverage: 149:225/277 of query aligns to 118:193/219 of 2ji5A

query
sites
2ji5A

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binding uridine 5'-triphosphate: H120 (= H151), T121 (≠ C152), T122 (= T153)

Sites not aligning to the query:

binding uridine 5'-triphosphate: 68, 71, 72, 115, 116, 117

3c1mC Cyrstal structure of threonine-sensitive aspartokinase from methanococcus jannaschii with mgamp-pnp and l-aspartate (see paper)
31% identity, 30% coverage: 129:212/277 of query aligns to 180:263/468 of 3c1mC

query
sites
3c1mC

L

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K

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binding phosphoaminophosphonic acid-adenylate ester: T229 (≠ S178), D230 (= D179), Y235 (= Y184), D238 (≠ N187), P239 (= P188), R240 (≠ H189)
binding aspartic acid: F192 (≠ A143), R206 (≠ E157), G207 (vs. gap), S209 (≠ N158)

Sites not aligning to the query:

2hmfA Structure of a threonine sensitive aspartokinase from methanococcus jannaschii complexed with mg-adp and aspartate (see paper)
31% identity, 30% coverage: 129:212/277 of query aligns to 180:263/464 of 2hmfA

query
sites
2hmfA

L

L

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G

G

binding adenosine-5'-diphosphate: T229 (≠ S178), D230 (= D179), V231 (= V180), Y235 (= Y184), T237 (≠ S186), D238 (≠ N187), P239 (= P188), R240 (≠ H189)
binding aspartic acid: F192 (≠ A143), R206 (≠ E157), G207 (vs. gap), S209 (≠ N158)

Sites not aligning to the query:

Query Sequence

>WP_017549551.1 NCBI__GCF_000330705.1:WP_017549551.1
MNRDILKECKRVVIKIGTNSIMKSPTKVDYHKIDRLAFVCSSLQQQGKEVVLVSSGAVGV
GASTLKMDKYPSAVAEQQALASVGQSALMNLYSRFFQHYEQYVGQILMTRDIINYPNSYA
NCNTSINSLLNKDIIPIINENDAVSVDESGHCTKFGENDTLAAVVAELVDADLLIIMSDV
DGLYDSNPHTNPEAELISYVGEIDTDILSMAGGAGSVFSTGGMETKLRAAQKMMSINKSM
IITSAKNPNIIFDILNGQDAGTLFKKNEAELQIQGDE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory