SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017549669.1 NCBI__GCF_000330705.1:WP_017549669.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

C0H3V2 Mannitol-specific phosphotransferase enzyme IIA component; EIIA; EIII; PTS system mannitol-specific EIIA component from Bacillus subtilis (strain 168) (see paper)
56% identity, 96% coverage: 1:136/141 of query aligns to 3:139/143 of C0H3V2

query
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C0H3V2

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S74 (= S72) modified: Phosphoserine

1j6tA Complex of enzyme iiamtl and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
40% identity, 96% coverage: 2:136/141 of query aligns to 4:141/144 of 1j6tA

query
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1j6tA

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binding phosphite ion: R46 (= R44), H62 (= H60), H108 (= H103)

P00550 PTS system mannitol-specific EIICBA component; EIICBA-Mtl; EII-Mtl; EC 2.7.1.197 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 96% coverage: 2:136/141 of query aligns to 496:633/637 of P00550

query
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P00550

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R538 (= R44) Stabilizes the transition state in the phosphoryl transfer from HPr to EIIA
H554 (= H60) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

384 modified: Phosphocysteine; by EIIA

O31645 PTS system mannose-specific EIIBCA component; EIIBCA-Man; EII-Man; EC 2.7.1.191 from Bacillus subtilis (strain 168) (see 2 papers)
30% identity, 97% coverage: 1:137/141 of query aligns to 505:647/650 of O31645

query
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O31645

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Sites not aligning to the query:

9 C→A: Appears to be unable to inactivate ManR, since ManR does not show any decrease in its DNA-binding activity in the presence of this mutant.
365 modified: Phosphoserine

P46321 Probable licABCH operon regulator; EC 2.7.1.- from Bacillus subtilis (strain 168) (see paper)
27% identity, 98% coverage: 2:139/141 of query aligns to 501:638/641 of P46321

query
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P46321

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H559 (= H60) mutation to G: Increase in activity.

Sites not aligning to the query:

219 H→A: Residual activity.; H→D: Loss of activity.
278 H→A: Loss of activity.; H→E: Loss of activity.
333 H→A: Loss of activity.
392 H→E: Loss of activity.; H→I: Residual activity.

Query Sequence

>WP_017549669.1 NCBI__GCF_000330705.1:WP_017549669.1
MLKKENIVLNQTAGSKEDVIRQAGQLLVDQGSVEPAYIDSMLAREEVVSTFMGNGLAIPH
GTDEGKTSVIESGLSLIQVPGGVDFDGNEAKVILGIAGKENEHLDMLQKIAVLFSDEDNA
ERVINASSADEIISLFENEDI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory