SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017550051.1 NCBI__GCF_000330705.1:WP_017550051.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
74% identity, 98% coverage: 5:322/324 of query aligns to 2:316/317 of P14193

query
sites
P14193

S

S

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N

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RQ 102:103 (= RQ 105:106) binding ATP
K198 (= K204) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R206) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ K208) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (= N210) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (= E213) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ DTGGT 234:238) binding D-ribose 5-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
71% identity, 97% coverage: 10:322/324 of query aligns to 1:299/299 of 1ibsB

query
sites
1ibsB

N

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binding methyl phosphonic acid adenosine ester: R96 (= R105), Q97 (= Q106), R99 (= R108)

1ibsA Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
72% identity, 96% coverage: 12:322/324 of query aligns to 1:297/297 of 1ibsA

query
sites
1ibsA

S

N

L

L

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I

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F

binding methyl phosphonic acid adenosine ester: R94 (= R105), Q95 (= Q106), R97 (= R108), H126 (= H139)
binding sulfate ion: D209 (= D234), T210 (= T235), A211 (≠ G236), T213 (= T238)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
70% identity, 96% coverage: 12:322/324 of query aligns to 1:295/295 of 1dkuA

query
sites
1dkuA

S

N

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x
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F

binding methyl phosphonic acid adenosine ester: R94 (= R105), H128 (= H139)
binding phosphomethylphosphonic acid adenosyl ester: K98 (= K109), S101 (≠ A112), R102 (= R113), D141 (= D152), S290 (= S317), V291 (= V318), S292 (= S319), F295 (= F322)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
54% identity, 97% coverage: 10:322/324 of query aligns to 4:315/318 of Q63XL8

query
sites
Q63XL8

N

H

S

D

S

G

L

L

R

M

L

V

F

F

S

T

L

G

K

N

G

A

N

N

E

P

P

A

L

L

A

A

E

Q

E

E

I

V

A

V

E

K

Q

I

I

L

G

G

I

I

P

P

L

L

G

G

K

K

I

A

T

M

V

V

Q

S

H

R

F

F

S

S

D

D

E

G

E

E

V

I

Q

Q

V

V

N

E

I

I

E

Q

E

E

S

N

V

V

R

R

G

G

C

K

D

D

V

V

F

F

V

V

V

L

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

T

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

L

M

I

I

M

M

I

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

A

M

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

S

P

R

R

-

S

A

A

R

R

E

V

P

A

I

I

T

S

A

A

K

K

L

V

V

V

A

A

N

N

L

M

I

L

E

E

T

I

A

A

G

G

A

V

D

E

R

R

V

I

I

I

S

T

L

M

D

D

L

L

H

H

A

A

P

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

I

V

D

D

H

N

L

I

M

Y

G

A

V

T

P

P

I

I

L

L

S

-

D

-

Y

-

F

L

L

G

E

D

H

L

K

R

D

K

L

Q

N

N

F

Y

D

P

E

D

V

L

V

V

S

S

P

P

D

D

H

V

G

G

V

V

T

V

R

R

A

A

R

R

K

A

M

L

A

A

D

K

R

Q

L

L

K

N

T

C

P

D

I

L

A

A

I

I

D

D

K

K

R

R

P

P

K

K

P

A

N

N

V

V

A

A

E

E

V

V

M

M

N

N

I

I

V

I

G

G

E

E

I

V

E

E

G

G

R

R

T

T

A

C

I

V

I

I

I

M

D

D

I

M

I

V

D

D

T

T

G

A

G

G

T

T

M

L

K

C

L

K

A

A

Q

Q

A

V

L

L

L

K

D

E

K

R

G

G

A

A

K

K

E

Q

V

V

Y

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

G

A

A

I

A

S

D

R

R

I

I

E

A

S

S

V

A

I

L

K

D

E

E

L

L

V

V

T

T

N

D

S

T

I

I

Q

P

L

L

P

S

E

A

E

E

K

S

-

L

K

A

I

C

D

P

K

K

I

I

V

R

G

A

L

L

S

S

V

S

G

A

E

G

L

L

M

L

A

A

Q

E

A

T

I

F

V

S

R

R

V

I

Y

R

E

R

E

G

E

D

S

S

V

V

S

M

I

S

L

L

F

F

RQ 99:100 (= RQ 105:106) binding ATP

6asvC E. Coli prpp synthetase (see paper)
50% identity, 96% coverage: 13:322/324 of query aligns to 2:311/311 of 6asvC

query
sites
6asvC

L

M

R

K

L

L

F

F

S

A

L

G

K

N

G

A

N

T

E

P

P

E

L

L

A

A

E

Q

E

R

I

I

A

A

E

N

Q

R

I

L

G

Y

I

T

P

S

L

L

G

G

K

D

I

A

T

A

V

V

Q

G

H

R

F

F

S

S

D

D

E

G

E

E

V

V

Q

S

V

V

N

Q

I

I

E

N

E

E

S

N

V

V

R

R

G

G

C

G

D

D

V

I

F

F

V

I

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

T

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

L

V

I

V

M

M

I

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

V

M

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

K

R

S

V

R

R

-

S

A

A

R

R

E

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

E

S

T

S

A

V

G

G

A

V

D

D

R

R

V

V

I

L

S

T

L

V

D

D

L

L

H

H

A

A

P

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

I

V

D

D

H

N

L

V

M

F

G

G

V

S

P

P

I

I

L

L

S

L

D

E

Y

D

F

M

L

L

E

Q

H

-

K

-

D

-

L

L

N

N

F

L

D

D

E

N

V

P

V

I

V

V

S

S

P

P

D
|
D

H

I

G

G

V

V

T

V

R

R

A

A

R

R

K

A

M

I

A

A

D

K

R

L

L

L

K

N

-

D

T

T

P

D

I

M

A

A

I

I

D

D

K

K

R

R

P

P

K

R

P

A

N

N

V

V

A

S

E

Q

V

V

M

M

N

H

I

I

V

I

G

G

E

D

I

V

E

A

G

G

R

R

T

D

A

C

I

V

I

L

I

V

D
|
D

I

M

I

I

D
|
D

T
|
T

G
|
G

G

G

T
|
T

M
x
L

K

C

L

K

A

A

Q

E

A

A

L

L

L

K

D

E

K

R

G

G

A

A

K

K

E

R

V

V

Y

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

S

N

R

N

I

L

E

R

E

N

S

S

V

V

I

I

K

D

E

E

L

V

V

V

T

C

N

D

S

T

I

I

Q

P

L

L

P

S

E

D

E

E

-

I

K

K

K

S

I

L

D

P

K

N

I

V

V

R

G

T

L

L

S

T

V

L

G

S

E

G

L

M

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

I

Y

S

E

N

E

E

S

S

V

I

S

S

I

A

L

M

F

F

binding phosphate ion: D168 (= D181), D218 (= D230), D219 (= D231), D222 (= D234), T223 (= T235), G224 (= G236), T226 (= T238), L227 (≠ M239)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
50% identity, 96% coverage: 13:323/324 of query aligns to 4:314/315 of P0A717

query
sites
P0A717

L

M

R

K

L

L

F

F

S

A

L

G

K

N

G

A

N

T

E

P

P

E

L

L

A

A

E

Q

E

R

I

I

A

A

E

N

Q

R

I

L

G

Y

I

T

P

S

L

L

G

G

K

D

I

A

T

A

V

V

Q

G

H

R

F

F

S

S

D

D

E

G

E

E

V

V

Q

S

V

V

N

Q

I

I

E

N

E

E

S

N

V

V

R

R

G

G

C

G

D

D

V

I

F

F

V

I

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

T

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

L

V

I

V

M

M

I

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

V

M

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

K

R

S

V

R

R

-

S

A

A

R

R

E

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

E

S

T

S

A

V

G

G

A

V

D

D

R

R

V

V

I

L

S

T

L

V

D
|
D

L

L

H

H

A

A

P

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

I

V

D

D

H

N

L

V

M

F

G

G

V

S

P

P

I

I

L

L

S

L

D

E

Y

D

F

M

L

L

E

Q

H

-

K

-

D

-

L

L

N

N

F

L

D

D

E

N

V

P

V

I

V

V

S

S

P

P

D

D

H

I

G

G

V

V

T

V

R

R

A

A

R

R

K

A

M

I

A

A

D

K

R

L

L

L

K

N

-

D

T

T

P

D

I

M

A

A

I

I

D

D

K

K

R

R

P

P

K

R

P

A

N

N

V

V

A

S

E

Q

V

V

M

M

N

H

I

I

V

I

G

G

E

D

I

V

E

A

G

G

R

R

T

D

A

C

I

V

I

L

I

V

D
|
D

I

M

I

I

D
|
D

T

T

G

G

T

T

M

L

K

C

L

K

A

A

Q

E

A

A

L

L

L

K

D

E

K

R

G

G

A

A

K

K

E

R

V

V

Y

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

S

N

R

N

I

L

E

R

E

N

S

S

V

V

I

I

K

D

E

E

L

V

V

V

T

C

N

D

S

T

I

I

Q

P

L

L

P

S

E

D

E

E

-

I

K

K

K

S

I

L

D

P

K

N

I

V

V

R

G

T

L

L

S

T

V

L

G

S

E

G

L

M

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

I

Y

S

E

N

E

E

S

S

V

I

S

S

I

A

L

M

F

F

D

E

D129 (= D137) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D230) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D231) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (= D234) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
52% identity, 95% coverage: 12:318/324 of query aligns to 1:299/300 of 3dahC

query
sites
3dahC

S

G

L

L

R

M

L

V

F

F

S

T

L

G

K

N

G

A

N

N

E

P

P

A

L

L

A

A

E

Q

E

E

I

V

A

V

E

K

Q

I

I

L

G

G

I

I

P

P

L

L

G

G

K

K

I

A

T

M

V

V

Q

S

H

R

F
|
F

S

S

D
|
D

E

G

E
|
E

V

I

Q

Q

V

V

N

E

I

I

E

Q

E

E

S

N

V

V

R

R

G

G

C

K

D

D

V

V

F

F

V

V

V

L

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

T

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

L

M

I

I

M

M

I

V

D

D

A

A

L

L

K

K

R

R

A

A

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

A

M

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

S

P

R

R

-

S

A

A

R

R

E

V

P

A

I

I

T

S

A

A

K

K

L

V

V

V

A

A

N

N

L

M

I

L

E

E

T

I

A

A

G

G

A

V

D

E

R

R

V

I

I

I

S

T

L

M

D

D

L

L

H

H

A

A

P

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

I

V

D

D

H

N

L

I

M

Y

G

A

V

T

P

P

I

I

L

L

S

-

D

-

Y

-

F

L

L

G

E

D

H

L

K

R

D

K

L

Q

N

N

F

Y

D

P

E

D

V

L

V

V

S

S

P

P

D

D

H

V

G

G

V

V

T

V

R

R

A

A

R

R

K

A

M

L

A

A

D

K

R

Q

L

L

K

N

T

C

P

D

I

L

A

A

I

I

D

D

K

K

R

R

P

-

K

-

P

-

N

-

V

-

A

-

E

-

V

V

M

M

N

N

I

I

V

I

G

G

E

E

I

V

E

E

G

G

R

R

T

T

A

C

I

V

I

I

I

M

D

D

I

M

I

V

D

D

T

T

G

A

G

G

T

T

M

L

K

C

L

K

A

A

Q

Q

A

V

L

L

L

K

D

E

K

R

G

G

A

A

K

K

E

Q

V

V

Y

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

G

A

A

I

A

S

D

R

R

I

I

E

A

S

S

V

A

I

L

K

D

E

E

L

L

V

V

T

T

N

D

S

T

I

I

Q

P

L

L

P

S

E

A

E

E

K

S

-

L

K

A

I

C

D

P

K

K

I

I

V

R

G

A

L

L

S

S

V

S

G

A

E

G

L

L

M

L

A

A

Q

E

A

T

I

F

V

S

R

R

V

I

Y

R

E

R

E

G

E

D

S

S

V

V

binding adenosine monophosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R105), Q95 (= Q106)

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
50% identity, 94% coverage: 13:318/324 of query aligns to 3:308/308 of 4s2uA

query
sites
4s2uA

L

M

R

K

L

L

F

F

S

A

L

G

K

N

G

A

N

T

E

P

P

E

L

L

A

A

E

Q

E

R

I

I

A

A

E

N

Q

R

I

L

G

Y

I

T

P

S

L

L

G

G

K

D

I

A

T

A

V

V

Q

G

H

R

F

F

S

S

D

D

E

G

E

E

V

V

Q

S

V

V

N

Q

I

I

E

N

E

E

S

N

V

V

R

R

G

G

C

G

D

D

V

I

F

F

V

I

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

T

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

L

V

I

V

M

M

I

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

V

M

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R

R

Q

Q

D

D

R

R

K

R

S

V

R

R

-

S

A

A

R

R

E

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

E

S

T

S

A

V

G

G

A

V

D

D

R

R

V

V

I

L

S

T

L

V

D

D

L

L

H

H

A

A

P

E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

I

V

D

D

H

N

L

V

M

F

G

G

V

S

P

P

I

I

L

L

S

L

D

E

Y

D

F

M

L

L

E

Q

H

-

K

-

D

-

L

L

N

N

F

L

D

D

E

N

V

P

V

I

V

V

S

S

P

P

D

D

H

I

G

G

V

V

T

V

R

R

A

A

R

R

K

A

M

I

A

A

D

K

R

L

L

L

K

N

-

D

T

T

P

D

I

M

A

A

I

I

D

D

K

K

R

R

P

P

K

R

P

A

N

N

V

V

A

S

E

Q

V

V

M

M

N

H

I

I

V

I

G

G

E

D

I

V

E

A

G

G

R

R

T

D

A

C

I

V

I

L

I

V

D

D

I

M

I

I

D
|
D

T

T

G

G

T
|
T

M

L

K

C

L

K

A

A

Q

E

A

A

L

L

L

K

D

E

K

R

G

G

A

A

K

K

E

R

V

V

Y

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

S

N

R

N

I

L

E

R

E

N

S

S

V

V

I

I

K

D

E

E

L

V

V

V

T

C

N

D

S

T

I

I

Q

P

L

L

P

S

E

D

E

E

-

I

K

K

K

S

I

L

D

P

K

N

I

V

V

R

G

T

L

L

S

T

V

L

G

S

E

G

L

M

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

I

Y

S

E

N

E

E

S

S

V

I

binding magnesium ion: D223 (= D234), T227 (= T238)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
49% identity, 96% coverage: 13:323/324 of query aligns to 2:306/307 of 7xmvA

query
sites
7xmvA

L

M

R

K

L

L

F

F

S

A

L

G

K

N

G

A

N

T

E

P

P

E

L

L

A

A

E

Q

E

R

I

I

A

A

E

N

Q

R

I

L

G

Y

I

T

P

S

L

L

G

G

K

D

I

A

T

A

V

V

Q

G

H

R

F
|
F

S

S

D
|
D

E

G

E
|
E

V

V

Q

S

V

V

N

Q

I

I

E

N

E

E

S

N

V

V

R

R

G

G

C

G

D

D

V

I

F

F

V

I

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

T

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

L

V

I

V

M

M

I

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

V

M

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q

Q

D

D

R
|
R

K

R

S
x
V

R
|
R

-

S

A

A

R

R

E

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

E

S

T

S

A

V

G

G

A

V

D

D

R

R

V

V

I

L

S

T

L

V

D

D

L

L

H
|
H

A

A

P
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

I

V

D

D

H

N

L

V

M
x
F

G

G

V
x
S

P

P

I

I

L

L

S

L

D

E

Y

D

F

M

L

L

E

Q

H

-

K

-

D

-

L

L

N

N

F

L

D

D

E

N

V

P

V

I

V

V

S

S

P

P

D

D

H

I

G

G

V

V

T
x
V

R

R

A

A

R

R

K
x
A

M

I

A

A

D

K

R

L

L

L

K

N

-

D

T

T

P

D

I

M

A

A

I

I

D

D

K

K

R

R

P

-

K

-

P

-

N

-

V

-

A

-

E

Q

V

V

M

M

N

H

I

I

V

I

G

G

E

D

I

V

E

A

G

G

R

R

T

D

A

C

I

V

I

L

I

V

D
|
D

I
x
M

I

I

D
|
D

T
|
T

G

G

G
|
G

T
|
T

M

L

K

C

L

K

A

A

Q

E

A

A

L

L

L

K

D

E

K

R

G

G

A

A

K

K

E

R

V

V

Y

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

S

N

R

N

I

L

E

R

E

N

S

S

V

V

I

I

K

D

E

E

L

V

V

V

T

C

N

D

S

T

I

I

Q

P

L

L

P

S

E

D

E

E

-

I

K

K

K

S

I

L

D

P

K

N

I

V

V

R

G

T

L

L

S

T

V

L

G

S

E

G

L

M

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

I

Y

S

E

N

E

E

S

S

V

I

S

S

I

A

L

M

F

F

D

E

binding adenosine-5'-diphosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R105), R97 (= R108), H129 (= H139)
binding adenosine monophosphate: R97 (= R108), V99 (≠ S110), R100 (= R111), E131 (≠ P141), F145 (≠ M155), S147 (≠ V157), V173 (≠ T186), A177 (≠ K190)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D230), D213 (= D231), M214 (≠ I232), D216 (= D234), T217 (= T235), G219 (= G237), T220 (= T238)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
49% identity, 96% coverage: 13:323/324 of query aligns to 2:306/307 of 7xmuA

query
sites
7xmuA

L

M

R

K

L

L

F

F

S

A

L

G

K

N

G

A

N

T

E

P

P

E

L

L

A

A

E

Q

E

R

I

I

A

A

E

N

Q

R

I

L

G

Y

I

T

P

S

L

L

G

G

K

D

I

A

T

A

V

V

Q

G

H

R

F
|
F

S

S

D
|
D

E

G

E
|
E

V

V

Q

S

V

V

N

Q

I

I

E

N

E

E

S

N

V

V

R

R

G

G

C

G

D

D

V

I

F

F

V

I

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

T

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

L

V

I

V

M

M

I

V

D

D

A

A

L

L

K

R

R

R

A

A

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

V

M

I

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

R

S

V

R
|
R

-

S

A

A

R

R

E

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

F

I

L

E

S

T

S

A

V

G

G

A

V

D

D

R

R

V

V

I

L

S

T

L

V

D

D

L

L

H
|
H

A

A

P
x
E

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

V

P

P

I

V

D

D

H

N

L

V

M
x
F

G

G

V
x
S

P

P

I

I

L

L

S

L

D

E

Y

D

F

M

L

L

E

Q

H

-

K

-

D

-

L

L

N

N

F

L

D

D

E

N

V

P

V

I

V

V

S

S

P

P

D
|
D

H

I

G

G

V

V

T
x
V

R

R

A

A

R

R

K

A

M

I

A

A

D

K

R

L

L

L

K

N

-

D

T

T

P

D

I

M

A

A

I

I

D

D

K

K

R

R

P

-

K

-

P

-

N

-

V

-

A

-

E

Q

V

V

M

M

N

H

I

I

V

I

G

G

E

D

I

V

E

A

G

G

R

R

T

D

A

C

I

V

I

L

I

V

D
|
D

D

D

I
x
M

I

I

D
|
D

T
|
T

G

G

T

T

M

L

K

C

L

K

A

A

Q

E

A

A

L

L

L

K

D

E

K

R

G

G

A

A

K

K

E

R

V

V

Y

F

A

A

C

Y

C

A

T

T

H

H

P

P

V

I

L

F

S

S

G

G

P

N

A

A

I

A

S

N

R

N

I

L

E

R

E

N

S

S

V

V

I

I

K

D

E

E

L

V

V

V

T

C

N

D

S

T

I

I

Q

P

L

L

P

S

E

D

E

E

-

I

K

K

K

S

I

L

D

P

K

N

I

V

V

R

G

T

L

L

S

T

V

L

G

S

E

G

L

M

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

I

Y

S

E

N

E

E

S

S

V

I

S

S

I

A

L

M

F

F

D

E

binding adenosine-5'-diphosphate: F33 (= F44), D35 (= D46), E37 (= E48), R94 (= R105), Q95 (= Q106), R97 (= R108), R97 (= R108), R100 (= R111), H129 (= H139), E131 (≠ P141), F145 (≠ M155), S147 (≠ V157), V173 (≠ T186)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D181), D212 (= D230), M214 (≠ I232), D216 (= D234), T217 (= T235)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
48% identity, 96% coverage: 13:322/324 of query aligns to 2:306/307 of 5t3oA

query
sites
5t3oA

L

L

R

L

L

I

F

F

S

S

L

G

K

Q

G

S

N

N

E

R

P

P

L

L

A

A

E

Q

E

A

I

I

A

A

E

E

Q

A

I

L

G

G

I

L

P

P

L

L

G

G

K

K

I

S

T

T

V

T

Q

L

H

R

F

F

S

A

D

N

E

D

E

N

V

L

Q

F

V

V

N

R

I

Y

E

E

S
|
S

V

L

R
|
R

G

E

C

G

D

D

V

V

F

F

V

I

V

V

Q

Q

P

S

T

F

S

V

Q

P

P

P

V

V

N

Q

E

D

H

H

L

L

M

M

Q

E

L

L

I

M

M

M

I

V

D

D

A

A

L

A

K

K

R

G

A

A

S

S

A

A

T

R

I

V

N

T

I

A

V

V

M

I

P

P

Y

Y

Y

F

G

S

Y

Y

A

A

R
|
R

Q
x
S

D

D

R

K

K

K

S
x
D

R

A

A

P

R

R

E

I

P

S

I

I

T

T

A

A

K

R

L

L

V

I

A

A

N

D

L

L

I

L

E

Q

T

T

A

A

G

G

A

A

D

D

R

R

V

V

I

L

S

T

L

M

D

T

L

L

H
|
H

A

S

P

P

Q

Q

I

V

Q

H

G

G

F

F

D

K

I

I

P

P

I

V

D

D

H

H

L

L

M

S

G

A

V

E

P

P

I

V

L

I

S

A

D

N

Y

Y

F

F

L

A

E

T

H

R

K

V

D

D

L

L

N

-

F

-

D

E

E

N

V

A

V

V

S

A

P

P

D

D

H

A

G

G

G

D

V

L

T

K

R

R

A

A

R

S

K

A

M

L

A

A

D

R

R

R

L

L

K

G

T

L

P

P

I

L

A

A

I

F

I

I

D

D

K

K

R

E

R
|
R

P

V

K

S

P

D

N

T

V

E

A

V

E

R

V

V

M

R

N

M

I

L

V

V

G

G

E

E

I

V

E

E

G

G

R

K

T

T

A

A

I

L

I

I

I

V

D

D

I

E

I

I

D

S

T

T

G

A

G

G

T

S

M

L

K

V

L

E

A

A

A

V

Q

E

A

A

L

L

L

M

D

Q

K

A

G

G

A

A

K

K

E

E

V

V

Y

Y

A

A

C

A

T

T

H

H

P

G

V

V

L

Y

S

V

G

G

P

P

A

A

I

L

S

D

R

R

I

I

E

A

E

K

S

S

V

P

I

V

K

K

E

E

L

V

V

A

T

T

N

D

S

T

I

C

Q

P

L

-

P

P

E

K

E

E

K

G

K

P

I

-

D

-

K

K

I

L

V

R

G

T

L

L

S

T

V

V

G

A

E

P

L

L

M

F

A

A

Q

E

A

A

I

I

V

W

R

R

V

I

Y

H

E

R

E

G

E

E

S

S

V

V

S

S

I

S

L

L

F

F

binding adenosine-5'-diphosphate: R94 (= R105), S95 (≠ Q106), D99 (≠ S110), H128 (= H139), R193 (= R206)
binding sulfate ion: S45 (= S56), R47 (= R58)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
48% identity, 96% coverage: 13:322/324 of query aligns to 3:307/312 of 7pn0A

query
sites
7pn0A

L

L

R

L

L

I

F

F

S

S

L

G

K

Q

G

S

N

N

E

R

P

P

L

L

A

A

E

Q

E

A

I

I

A

A

E

E

Q

A

I

L

G

G

I

L

P

P

L

L

G

G

K

K

I

S

T

T

V

T

Q

L

H

R

F

F

S

A

D

N

E

D

E

N

V

L

Q

F

V

V

N

R

I

Y

E

E

S

S

V

L

R

R

G

E

C

G

D

D

V

V

F

F

V

I

V

V

Q

Q

P

S

T

F

S

V

Q

P

P

P

V

V

N

Q

E

D

H

H

L

L

M

M

Q

E

L

L

I

M

M

M

I

V

D

D

A

A

L

A

K

K

R

G

A

A

S

S

A

A

T

R

I

V

N

T

I

A

V

V

M

I

P

P

Y

Y

Y

F

G

S

Y

Y

A

A

R
|
R

Q
x
S

D

D

R

K

K

K

S

D

R

A

A

P

R

R

E

I

P

S

I

I

T

T

A

A

K

R

L

L

V

I

A

A

N

D

L

L

I

L

E

Q

T

T

A

A

G

G

A

A

D

D

R

R

V

V

I

L

S

T

L

M

D

T

L

L

H
|
H

A

S

P

P

Q

Q

I

V

Q

H

G

G

F

F

D

K

I

I

P

P

I

V

D

D

H

H

L

L

M

S

G

A

V

E

P

P

I

V

L

I

S

A

D

N

Y

Y

F

F

L

A

E

T

H

R

K

V

D

D

L

L

N

-

F

-

D

E

E

N

V

A

V

V

S

A

P

P

D

D

H

A

G

G

G

D

V

L

T

K

R

R

A

A

R

S

K

A

M

L

A

A

D

R

R

R

L

L

K

G

T

L

P

P

I

L

A

A

I

F

I

I

D

D

K

K

R

E

R

R

P

V

K

S

P

D

N

T

V

E

A

V

E

R

V

V

M

R

N

M

I

L

V

V

G

G

E

E

I

V

E

E

G

G

R

K

T

T

A

A

I

L

I

I

I

V

D

D

I

E

I

I

D

S

T

T

G

A

G

G

T

S

M

L

K

V

L

E

A

A

A

V

Q

E

A

A

L

L

L

M

D

Q

K

A

G

G

A

A

K

K

E

E

V

V

Y

Y

A

A

C

A

T

T

H

H

P

G

V

V

L

Y

S

V

G

G

P

P

A

A

I

L

S

D

R

R

I

I

E

A

E

K

S

S

V

P

I

V

K

K

E

E

L

V

V

A

T

T

N

D

S

T

I

C

Q

P

L

-

P

P

E

K

E

E

K

G

K

P

I

-

D

-

K

K

I

L

V

R

G

T

L

L

S

T

V

V

G

A

E

P

L

L

M

F

A

A

Q

E

A

A

I

I

V

W

R

R

V

I

Y

H

E

R

E

G

E

E

S

S

V

V

S

S

I

S

L

L

F

F

binding adenosine-5'-diphosphate: R95 (= R105), S96 (≠ Q106), H129 (= H139)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
45% identity, 97% coverage: 10:322/324 of query aligns to 3:316/321 of O94413

query
sites
O94413

N

S

S

N

S

S

L

I

R

K

L

I

F

F

S

A

L

G

K

N

G

S

N

H

E

P

P

E

L

L

A

A

E

E

E

K

I

V

A

A

E

R

Q

R

I

I

G

G

I

L

P

S

L

L

G

G

K

K

I

V

T

A

V

V

Q

V

H

Q

F

Y

S

S

D

N

E

R

E

E

V

T

Q

S

V

V

N

T

I

I

E

G

E

E

S

S

V

V

R

R

G

D

C

E

D

D

V

V

F

F

V

I

V

L

Q

Q

P

T

T

G

S

C

Q

G

P

S

V

I

N

N

E

D

H

H

L

L

M

M

Q

E

L

L

I

I

M

M

I

I

D

N

A

A

L

C

K

R

R

S

A

A

S

S

A

A

A

R

T

R

I

I

N

T

I

A

V

I

M

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

K

K

S

D

R

K

A

S

R

R

E

A

P

P

I

I

T

T

A

A

K

R

L

L

V

V

A

A

N

N

L

M

I

L

E

Q

T

T

A

A

G

G

A

C

D

N

R

H

V

I

I

I

S

T

L

M

D

D

L

L

H

H

A

A

P

S

Q

Q

I

I

Q

Q

G

G

F

F

D

N

I

V

P

P

I

V

D

D

H

N

L

L

M

Y

G

A

V

E

P

P

I

S

L

V

S

L

D

R

Y

Y

F

I

L

R

E

E

H

N

K

I

D

D

L

T

N

T

F

V

D

N

E

P

V

T

V

V

V

I

V

V

S
|
S

P

P

D

D

H

A

G

G

V

A

T

K

R

R

A

A

R

T

K

A

M

L

A

A

D

D

R

R

L

L

K

D

T

L

P

D

I

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

Q

K

K

P

A

N

N

V

E

A

V

E

S

V

R

M

M

N

V

I

L

V

V

G

G

E

D

I

V

E

R

G

D

R

K

T

L

A

A

I

I

I

L

I

V

D

D

I

M

I

A

D

D

T

T

G

C

G

G

T

T

M

L

K

G

L

L

A

A

Q

K

A

T

L

L

L

K

D

D

K

N

G

G

A

A

K

K

E

A

V

V

Y

Y

A

A

C

I

C

V

T

T

H

H

P

G

V

I

L

L

S

S

G

G

P

K

A

A

I

I

S

K

R

V

I

I

E

N

E

E

S

S

V

A

I

L

K

E

E

K

L

V

V

I

V

V

T

T

N

N

S

T

I

I

Q

P

L

H

P

D

E

D

E

K

K

R

K

S

I

L

-

C

D

S

K

K

I

I

V

E

G

T

L

I

S

D

V

I

G

S

E

G

L

V

M

L

A

A

Q

E

A

C

I

I

V

R

R

R

V

I

Y

H

E

H

E

G

E

E

S

S

V

V

S

S

I

V

L

L

F

F

S172 (= S179) modified: Phosphoserine

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
46% identity, 96% coverage: 12:322/324 of query aligns to 3:313/318 of P60891

query
sites
P60891

S

N

L

I

R

K

L

I

F

F

S

S

L

G

K

S

G

S

N

H

E

Q

P

D

L

L

A
x
S

E

Q

E

K

I

I

A

A

E

D

Q

R

I

L

G

G

I

L

P

E

L

L

G

G

K

K

I

V

T

V

V

T

Q

K

H

K

F

F

S

S

D

N

E

Q

E

E

V

T

Q

C

V

V

N

E

I

I

E

G

E

E

S

S

V

V

R

R

G

G

C

E

D
|
D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

S

C

Q

G

P

E

V

I

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

I

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

A

S

T

R

I

V

N

T

I

A

V

V

M

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

K

K

S

D

R

K

A

S

R

R

E

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N
|
N

L

M

I

L

E

S

T

V

A

A

G

G

A

A

D

D

R

H

V

I

I

I

S

T

L

M

D

D

L
|
L

H

H

A

A

P
x
S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

I
x
V

D

D

H
x
N

L

L

M
x
Y

G

A

V

E

P

P

I

A

L

V

S

L

D

K

Y

W

F

I

L

R

E

E

H

N

K

I

D

S

L

-

N

E

F

W

D

R

E

N

V

C

V

T

V

I

V

V

S

S

P

P

D

D

H

A

G

G

V

A

T

K

R

R

A

V

R

T

K

S

M

I

A

A

D
|
D

R

R

L

L

K

N

T

V

P

D

I

F

A
|
A

I

L

I

I

D
x
H

K

K

R

E

R

R

P

K

K

K

P

A

N

N

V

E

A

V

E
x
D

V

R

M

M

N

V

I

L

V

V

G

G

E

D

I

V

E

K

G

D

R

R

T

V

A

A

I

I

I

L

I
x
V

D

D

I

M

I

A

D

D

T

T

G

C

G

G

T

T

M

I

K

C

L
x
H

A

A

Q

D

A

K

L

L

D

S

K

A

G

G

A

A

K

T

E

R

V

V

Y

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

S

S

R

R

I

I

E

N

S

A

V

C

I

F

K

E

E

A

L

V

V

V

T

T

N

N

S

T

I

I

Q

-

L

-

P

P

E

Q

E

E

K

D

K

K

I

M

-

K

-

H

-

C

D

S

K

K

I

I

V

Q

G

V

L

I

S

D

V

I

G

S

E

M

L

I

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

T

Y

H

E

N

E

G

E

E

S

S

V

V

S

S

I

Y

L

L

F

F

S16 (≠ A25) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (= D61) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (= N123) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (= L138) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ P141) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ I151) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (≠ H153) mutation to H: No effect on catalytic activity.
Y146 (≠ M155) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (= D193) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (= A200) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ D203) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E213) to H: in a breast cancer sample; somatic mutation
V219 (≠ I229) to G: in a breast cancer sample; somatic mutation
H231 (≠ L241) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
46% identity, 96% coverage: 12:322/324 of query aligns to 2:312/316 of 8dbkB

query
sites
8dbkB

S

N

L

I

R

K

L

I

F

F

S

S

L

G

K

S

G

S

N

H

E

Q

P

D

L

L

A

S

E

Q

E

K

I

I

A

A

E

D

Q

R

I

L

G

G

I

L

P

E

L

L

G

G

K

K

I

V

T

V

V

T

Q

K

H

K

F
|
F

S

S

D
x
N

E

Q

E
|
E

V

T

Q

C

V

V

N

E

I

I

E

G

E

E

S
|
S

V

V

R
|
R

G

G

C

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

S

C

Q

G

P

E

V

I

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

I

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

A

S

T

R

I

V

N

T

I

A

V

V

M

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

K

K

K

S

D

R

K

A

S

R

R

E

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

E

S

T

V

A

A

G

G

A

A

D

D

R

H

V

I

I

I

S

T

L

M

D

D

L

L

H
|
H

A

A

P

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

I

V

D

D

H

N

L

L

M

Y

G

A

V

E

P

P

I

A

L

V

S

L

D

K

Y

W

F

I

L

R

E

E

H

N

K

I

D

S

L

-

N

E

F

W

D

R

E

N

V

C

V

T

V

I

V

V

S

S

P

P

D
|
D

H

A

G
|
G

G

G

V

A

T

K

R

R

A

V

R

T

K

S

M

I

A

A

D

D

R

R

L

L

K

N

T

V

P

D

I

F

A

A

I

L

I

I

D

H

K
|
K

R

E

R
|
R

P

K

K

K

P

A

N
|
N

V

E

A

V

E

D

V

R

M

M

N

V

I

L

V

V

G

G

E

D

I

V

E

K

G

D

R

R

T

V

A

A

I

I

I

L

I

V

D
|
D

I

M

I

A

D
|
D

T
|
T

G
x
C

G
|
G

T
|
T

M

I

K

C

L

H

A

A

Q

D

A

K

L

L

D

S

K

A

G

G

A

A

K

T

E

R

V

V

Y

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

S

S

R

R

I

I

E

N

S

A

V

C

I

F

K

E

E

A

L

V

V

V

T

T

N

N

S

T

I

I

Q

-

L

-

P

P

E

Q

E

E

K

D

K

K

I

M

-

K

-

H

-

C

D

S

K

K

I

I

V

Q

G

V

L

I

S

D

V

I

G

S

E

M

L

I

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

T

Y

H

E

N

E

G

E

E

S

S

V

V

S

S

I

Y

L

L

F

F

binding adenosine monophosphate: R95 (= R105), Q96 (= Q106), N199 (= N210)
binding adenosine-5'-triphosphate: F34 (= F44), N36 (≠ D46), E38 (= E48)
binding phosphate ion: S46 (= S56), R48 (= R58)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H139), D170 (= D181), G172 (= G183), K193 (= K204), R195 (= R206), D219 (= D230), D220 (= D231), D223 (= D234), T224 (= T235), C225 (≠ G236), G226 (= G237), T227 (= T238)

8dbeA Human prps1 with adp; hexamer (see paper)
46% identity, 96% coverage: 12:322/324 of query aligns to 2:312/316 of 8dbeA

query
sites
8dbeA

S

N

L

I

R

K

L

I

F

F

S

S

L

G

K

S

G

S

N

H

E

Q

P

D

L

L

A

S

E

Q

E

K

I

I

A

A

E

D

Q

R

I

L

G

G

I

L

P

E

L

L

G

G

K

K

I

V

T

V

V

T

Q

K

H

K

F
|
F

S

S

D
x
N

E

Q

E
|
E

V

T

Q

C

V

V

N

E

I

I

E

G

E

E

S

S

V

V

R

R

G

G

C

E

D

D

V

V

F

Y

V

I

V

V

Q

Q

P

S

T

G

S

C

Q

G

P

E

V

I

N

N

E

D

H

N

L

L

M

M

Q

E

L

L

I

I

M

M

I

I

D

N

A

A

L

C

K

K

R

I

A

A

S

S

A

A

A

S

T

R

I

V

N

T

I

A

V

V

M

I

P

P

Y

C

Y

F

G

P

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
x
K

K
|
K

S
x
D

R

K

A
x
S

R
|
R

E

A

P

P

I

I

T

S

A

A

K

K

L

L

V

V

A

A

N

N

L

M

I

L

E

S

T

V

A

A

G

G

A

A

D

D

R

H

V

I

I

I

S

T

L

M

D

D

L

L

H
|
H

A

A

P

S

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

I

P

P

I

V

D
|
D

H

N

L

L

M
x
Y

G

A

V

E

P

P

I

A

L

V

S

L

D

K

Y

W

F

I

L

R

E

E

H

N

K

I

D

S

L

-

N

E

F

W

D

R

E

N

V

C

V

T

V

I

V

V

S

S

P

P

D
|
D

H

A

G

G

V

A

T

K

R

R

A

V

R

T

K

S

M

I

A

A

D

D

R

R

L

L

K

N

T

V

P

D

I

F

A

A

I

L

I

I

D

H

K

K

R

E

R

R

P

K

K

K

P

A

N

N

V

E

A

V

E

D

V

R

M

M

N

V

I

L

V

V

G

G

E

D

I

V

E

K

G

D

R

R

T

V

A

A

I

I

I

L

I

V

D
|
D

I

M

I

A

D
|
D

T
|
T

G

C

G
|
G

T
|
T

M

I

K

C

L

H

A

A

Q

D

A

K

L

L

D

S

K

A

G

G

A

A

K

T

E

R

V

V

Y

Y

A

A

C

I

C

L

T

T

H

H

P

G

V

I

L

F

S

S

G

G

P

P

A

A

I

I

S

S

R

R

I

I

E

N

S

A

V

C

I

F

K

E

E

A

L

V

V

V

T

T

N

N

S

T

I

I

Q

-

L

-

P

P

E

Q

E

E

K

D

K

K

I

M

-

K

-

H

-

C

D

S

K

K

I

I

V

Q

G

V

L

I

S

D

V

I

G

S

E

M

L

I

M

L

A

A

Q

E

A

A

I

I

V

R

R

R

V

T

Y

H

E

N

E

G

E

E

S
|
S

V
|
V

S
|
S

I

Y

L

L

F
|
F

binding adenosine-5'-diphosphate: F34 (= F44), N36 (≠ D46), E38 (= E48), R95 (= R105), Q96 (= Q106), K98 (≠ R108), K99 (= K109), D100 (≠ S110), S102 (≠ A112), R103 (= R113), H129 (= H139), D142 (= D152), Y145 (≠ M155), S307 (= S317), V308 (= V318), S309 (= S319), F312 (= F322)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H139), D170 (= D181), D219 (= D230), D220 (= D231), D223 (= D234), T224 (= T235), G226 (= G237), T227 (= T238)

6nfeB Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
49% identity, 93% coverage: 11:312/324 of query aligns to 1:297/299 of 6nfeB

query
sites
6nfeB

S

S

S

T

L

M

R

M

L

I

F

F

S

T

L

G

K

N

G

A

N

N

E

P

P

E

L

L

A

A

E

L

E

K

I

I

A

S

E

S

Q

H

I

L

G

Q

I

I

P

P

L

I

G

G

K

K

I

A

T

T

V

V

Q

G

H

T

F
|
F

S

S

D
|
D

E

G

E
|
E

V

T

Q

M

V

V

N

E

I

I

E

L

E

E

S

N

V

V

R

R

G

G

C

K

D

D

V

V

F

F

V

V

V

L

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

A

N

N

E

N

H

N

L

L

M

M

Q

E

L

L

I

I

M

M

I

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

V

M

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R

R

K

R

S

V

R

R

-

S

A

A

R

R

E

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

I

M

E

A

T

S

A

V

G

G

A

I

D

C

R

R

V

V

I

L

S

T

L

V

D

D

L

L

H
|
H

A

A

P

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

I

V

D

D

H

N

L

V

M

Y

G

S

V

T

P

P

I

V

L

L

S

L

D

E

Y

D

F

I

L

T

E

K

H

Q

K

K

D

-

L

-

N

-

F

L

D

N

E

N

V

I

V

M

V

I

V

V

S

S

P

P

D

D

H

V

G

G

V

V

T

V

R

R

A

A

R

R

K

A

M

V

A

A

D

K

R

R

L

L

K

N

-

D

T

A

P

E

I

L

A

S

I

I

D

D

K

K

R

R

P

-

K

-

P

-

N

-

V

-

A

S

E

E

V

V

M

M

N

H

I

I

V

I

G

G

E

E

I

P

E

A

G

N

R

K

T

N

A

C

I

I

I

V

D
|
D

I
|
I

I

V

D
|
D

T
|
T

G
x
A

G

G

T
|
T

M

L

K

C

L

T

A

A

Q

H

A

E

L

L

L

K

D

K

K

N

G

G

A

A

K

K

E

S

V

V

Y

R

A

A

C

Y

C

I

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

S

N

R

N

I

I

E

K

E

H

S

S

V

G

I

L

K

D

E

E

L

V

V

V

T

T

N

D

S

T

I

I

Q

P

L

L

P

S

-

A

E

E

E

A

K

Q

K

N

I

C

D

E

K

K

I

I

V

R

G

V

L

V

S

S

V

L

G

A

E

D

L

M

M

L

A

A

Q

Q

A

A

I

I

V

K

R

R

V

V

binding adenosine-5'-diphosphate: F34 (= F44), D36 (= D46), E38 (= E48), R95 (= R105), Q96 (= Q106), H130 (= H139)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H139), D214 (= D230), D215 (= D231), I216 (= I232), D218 (= D234), T219 (= T235), A220 (≠ G236), T222 (= T238)

6nfeA Crystal structure of ribose-phosphate pyrophosphokinase from legionella pneumophila with bound amp, adp, and ribose-5-phosphate
49% identity, 93% coverage: 11:312/324 of query aligns to 1:296/298 of 6nfeA

query
sites
6nfeA

S

S

S

T

L

M

R

M

L

I

F

F

S

T

L

G

K

N

G

A

N

N

E

P

P

E

L

L

A

A

E

L

E

K

I

I

A

S

E

S

Q

H

I

L

G

Q

I

I

P

P

L

I

G

G

K

K

I

A

T

T

V

V

Q

G

H

T

F
|
F

S

S

D
|
D

E

G

E
|
E

V

T

Q

M

V

V

N

E

I

I

E

L

E

E

S

N

V

V

R

R

G

G

C

K

D

D

V

V

F

F

V

V

V

L

Q

Q

P

S

T

T

S

C

Q

A

P

P

V

A

N

N

E

N

H

N

L

L

M

M

Q

E

L

L

I

I

M

M

I

A

D

D

A

A

L

L

K

R

R

R

A

S

S

S

A

A

A

G

T

R

I

I

N

T

I

A

V

V

M

V

P

P

Y

Y

Y

F

G

G

Y

Y

A

A

R
|
R

Q
|
Q

D

D

R
|
R

K

R

S
x
V

R

R

-

S

A

A

R

R

E

V

P

P

I

I

T

T

A

A

K

K

L

V

V

V

A

A

N

D

L

M

I

M

E

A

T

S

A

V

G

G

A

I

D

C

R

R

V

V

I

L

S

T

L

V

D

D

L

L

H
|
H

A

A

P

D

Q

Q

I

I

Q

Q

G

G

F

F

D

Y

I

M

P

P

I

V

D

D

H

N

L

V

M
x
Y

G

S

V

T

P

P

I

V

L

L

S

L

D

E

Y

D

F

I

L

T

E

K

H

Q

K

K

D

-

L

-

N

-

F

L

D

N

E

N

V

I

V

M

V

I

V

V

S

S

P

P

D

D

H

V

G

G

V

V

T

V

R
|
R

A

A

R

R

K
x
A

M

V

A

A

D
x
K

R

R

L

L

K

N

-

D

T

A

P

E

I

L

A

S

I

I

D

D

K

K

R

R

P

-

K

-

P

-

N

-

V

-

A

-

E

E

V

V

M

M

N

H

I

I

V

I

G

G

E

E

I

P

E

A

G

N

R

K

T

N

A

C

I

I

I

V

D
|
D

I
|
I

I

V

D
|
D

T
|
T

G
x
A

G

G

T
|
T

M

L

K

C

L

T

A

A

Q

H

A

E

L

L

L

K

D

K

K

N

G

G

A

A

K

K

E

S

V

V

Y

R

A

A

C

Y

C

I

T

T

H

H

P

P

V

V

L

L

S

S

G

G

P

P

A

A

I

V

S

N

R

N

I

I

E

K

E

H

S

S

V

G

I

L

K

D

E

E

L

V

V

V

T

T

N

D

S

T

I

I

Q

P

L

L

P

S

-

A

E

E

E

A

K

Q

K

N

I

C

D

E

K

K

I

I

V

R

G

V

L

V

S

S

V

L

G

A

E

D

L

M

M

L

A

A

Q

Q

A

A

I

I

V

K

R

R

V

V

binding adenosine-5'-diphosphate: F34 (= F44), D36 (= D46), E38 (= E48), R95 (= R105), Q96 (= Q106), H130 (= H139)
binding adenosine monophosphate: R98 (= R108), V100 (≠ S110), Y146 (≠ M155), R175 (= R187), A178 (≠ K190), K181 (≠ D193)
binding 5-O-phosphono-alpha-D-ribofuranose: H130 (= H139), D213 (= D230), D214 (= D231), I215 (= I232), D217 (= D234), T218 (= T235), A219 (≠ G236), T221 (= T238)

P9WKE3 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 96% coverage: 12:323/324 of query aligns to 11:323/326 of P9WKE3

query
sites
P9WKE3

S

N

L

L

R

M

L

L

F

F

S

A

L

G

K

R

G

A

N

H

E

P

P

E

L

L

A

A

E

E

E

Q

I

V

A

A

E
x
K

Q

E

I

L

G

D

I

V

P

H

L

V

G

T

K

S

I

Q

T

D

V

A

Q

R

H

E

F

F

S

A

D

N

E

G

E

E

V

I

Q

F

V

V

N

R

I

F

E

H

E

E

S

S

V

V

R

R

G

G

C

C

D

D

V

A

F

F

V

V

V

L

Q

Q

P

S

T

C

S

P

Q

A

P

P

V

V

N

N

E

R

H

W

L

L

M

M

Q

E

L

Q

L

L

I

I

M

M

I

I

D

D

A

A

L

L

K

K

R

R

A

G

S

S

A

A

A

K

T

R

I

I

N

T

I

A

V

V

M

M

P

P

Y

F

Y

Y

G

P

Y

Y

A

A

R

R

Q

Q

D

D

R

K

K

K

S

H

R

R

A

G

R

R

E

E

P

P

I

I

T

S

A

A

K

R

L

L

V

I

A

A

N

D

L

L

I

L

E

K

T

T

A

A

G

G

A

A

D

D

R

R

V

I

I

V

S

T

L

V

D

D

L

L

H

H

A

T

P

D

Q

Q

I

I

Q

Q

G

G

F

F

D

D

I

G

P

P

I

V

D

D

H

H

L

M

M

R

G

G

V

Q

P

N

I

L

L

L

S

T

D

G

Y

Y

F

I

L

R

E

D

-

N

H

Y

K

P

D

D

L

G

N

N

F

-

D

-

E

-

V

M

V

V

S

S

P

P

D

D

H

S

G

G

G

R

V

V

T

R

R

I

A

A

R

E

K

K

M

W

A

A

D

D

R

A

L

L

-

G

K

G

T

V

P

P

I

L

A

A

I

F

I

I

D

H

K

K

R

T

R

R

-

D

P

P

K

R

-

V

P

P

N

N

V

Q

A

V

E

V

V

S

M

N

N

R

I

V

V

V

G

G

E

D

I

V

E

A

G

G

R

R

T

T

A

C

I

V

I

L

I

I

D

D

I

M

I

I

D

D

T

T

G

G

T

T

M

I

K

A

L

G

A

A

A

V

Q

A

A

L

L

L

L

H

D

N

K

D

G

G

A

A

K

G

E

D

V

V

Y

I

A

I

C

A

T

T

H

H

P

G

V

V

L

L

S

S

G

D

P

P

A

A

I

A

S

Q

R

R

I

L

E

A

E

S

S

C

V

G

I

A

K

R

E

E

L

V

V

I

V

V

T

T

N

N

S

T

I

L

Q

P

L

I

P

G

E

E

E

D

K

K

K

R

I

F

D

P

K

Q

I

L

V

T

G

V

L

L

S

S

V

I

G

A

E

P

L

L

M

L

A

A

Q

S

A

T

I

I

V

R

R

A

V

V

Y

F

E

E

E

N

E

G

S

S

V

V

S

T

I

G

L

L

F

F

D

D

K29 (≠ E30) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Query Sequence

>WP_017550051.1 NCBI__GCF_000330705.1:WP_017550051.1
MLASSNQYKNSSLRLFSLKGNEPLAEEIAEQIGIPLGKITVQHFSDEEVQVNIEESVRGC
DVFVVQPTSQPVNEHLMQLLIMIDALKRASAATINIVMPYYGYARQDRKSRAREPITAKL
VANLIETAGADRVISLDLHAPQIQGFFDIPIDHLMGVPILSDYFLEHKDLNFDEVVVVSP
DHGGVTRARKMADRLKTPIAIIDKRRPKPNVAEVMNIVGEIEGRTAIIIDDIIDTGGTMK
LAAQALLDKGAKEVYACCTHPVLSGPAISRIEESVIKELVVTNSIQLPEEKKIDKIVGLS
VGELMAQAIVRVYEEESVSILFDT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory