SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017596912.1 NCBI__GCF_000341125.1:WP_017596912.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
44% identity, 99% coverage: 5:386/386 of query aligns to 56:453/453 of Q9VLJ8

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Q9VLJ8

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T62 (≠ D11) modified: Phosphothreonine

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
40% identity, 97% coverage: 13:386/386 of query aligns to 39:440/440 of P38820

query
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P38820

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C225 (= C197) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.
C397 (= C344) mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
37% identity, 96% coverage: 16:386/386 of query aligns to 64:482/482 of O59954

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G82 (= G34) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G52) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R82) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G137) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ D167) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (≠ A208) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
55% identity, 66% coverage: 14:268/386 of query aligns to 4:269/271 of Q72J02

query
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Q72J02

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I

I

L

P

P

D

Q

V

V

G

G

M

P

D

E

G

G

Q

Q

K

E

R

R

L

L

K

K

N

R

A

A

K

S

V

V

L

V

V

V

G

G

A

A

G

G

L

L

G

G

S

V

P

P

A

V

L

L

L

Q

Y

Y

L

L

A

V

A

A

G

G

V

V

G

G

T

R

L

V

G

G

I

V

D

E

F

M

D

D

V

R

V

V

D

E

E

V

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

I

L

H

Y

G

T

Q

T

S

E

D

D

V

V

D

G

R

E

P

P

K

K

A

A

E

L

S

V

A

A

R

Q

D

K

S

R

I

L

K

Q

E

A

V

L

N

N

P

P

N

L

V

V

E

R

V

V

I

E

L

A

H

Y

Q

P

E

V

R

R

L

L

E

T

S

S

D

E

N

N

A

A

L

L

D

E

I

I

F

L

A

R

G

P

Y

Y

D

D

L

L

I

V

L

V

D

D

G

A

T

S

D

D

N

N

F

F

A

P

T

T

R

R

Y

Y

L

L

V

V

N

N

D

D

A

A

C

A

V

V

L

L

D

G

K

K

P

P

Y

L

V

V

W

F

G

G

S

A

I

I

Y

Y

R

Q

F

F

D

D

G

G

Q

Q

V

V

S

A

V

V

F

F

W

H

N

H

-

P

-

T

-

L

-

H

-

G

E

E

H

M

G

G

P

P

Q

C

Y

Y

R

R

D

C

L

L

Y

F

P

P

E

K

P

P

G

G

M

A

V

V

P

P

S

S

C
|
C

A

A

E

E

G

A

G

G

V

V

L

F

G

G

V

V

L

L

C

P

A

A

S

V

I

V

G

G

S

S

V

L

M

M

V

A

N

A

E

E

A

A

I

L

K

K

L

V

I

L

T

L

G

G

I

I

G

G

D

K

P

P

L

L

V

A

G

G

R

H

L

L

I

L

F

Y

D

D

A

A

L

L

E

E

M

A

T

S

W

F

R

R

E

K

V

L

K

T

V

V

R

R

K

R

D

N

P

P

D

R

T

C

-

P

-

V

-

C

-

G

-

D

E

E

P

P

V

T

T

Q

-

R

E

E

L

L

I

V

D

D

Y

Y

E

E

A

A

F

F

C
|
C

G

G

C192 (= C197) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.
C268 (= C267) mutation to S: Still able to form a thioester complex with TtuB.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
38% identity, 98% coverage: 9:386/386 of query aligns to 51:460/460 of O95396

query
sites
O95396

P

P

A

K

D

A

E

A

L

L

T

S

V

R

D

D

E

E

V

I

R

L

R

R

Y

Y

S

S

R

R

H

Q

L

L

I

V

I

L

P

P

D

E

V

L

G

G

M

V

D

H

G

G

Q

Q

K

L

R

R

L

L

K

G

N

T

A

A

K

C

V

V

L

L

V

I

V

V

G

G

A

C

G

G

L

L

G

G

S

C

P

P

A

L

L

A

L

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

T

R

L

L

G

G

I

L

I

V

D

D

F

Y

D

D

V

V

D

E

E

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

I

V

I

L

H

H

G

G

Q

E

S

A

D

L

V

A

D

G

R

Q

P

A

K

K

A

A

E

F

S

S

A

A

R

A

D

A

S

S

I

L

K

R

E

R

V

L

N

N

P

S

N

A

V

V

E

E

V

C

I

V

L

P

H

Y

Q
x
T

E
x
Q

R
x
A

L
|
L

E
x
T

S
x
P

D
x
A

N
x
T

A
|
A

L
|
L

D
|
D

I
x
L

F
x
V

A
x
R

G
x
R

Y
|
Y

D
|
D

L
x
V

I
x
V

L
x
A

D
|
D

G
x
C

T
x
S

D
|
D

N
|
N

F
x
V

A
x
P

T
|
T

R
|
R

Y
|
Y

L
|
L

V
|
V

N
|
N

D
|
D

A
|
A

C
|
C

V
|
V

L
|
L

L
x
A

D
x
G

K
x
R

P
|
P

Y
x
L

V
|
V

W
x
S

G
x
A

S
|
S

I
x
A

Y
x
L

R
|
R

F
|
F

D
x
E

G
|
G

Q
|
Q

V
x
I

S
x
T

V
|
V

F
x
Y

W
x
H

N
x
Y

E
x
D

H
x
G

G
|
G

P
|
P

Q
x
C

Y
|
Y

R
|
R

D
x
C

L
x
I

Y
x
F

P
|
P

E
x
Q

P
|
P

P
x
A

G
x
E

M
x
T

V
|
V

P
x
T

S
x
N

C
|
C

A

A

E

D

G

G

V

V

L

L

G

G

V

V

L

V

C

T

A

G

S

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I

L

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G

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C

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L

M

Q

V

A

N

L

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E

A

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L

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K

L

I

I

A

T

A

G

G

I

L

G

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D

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P

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G

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S

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L

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L

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F

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D

A

A

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G

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F

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L

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D

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P

R

-

L

-

D

-

C

-

A

-

C

-

G

D

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T

R

E

P

P

T

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V

T

T

E

D

L

L

I

L

D

D

Y

Y

E

E

A

A

F

F

C
|
C

G

G

A

S

V

S

S

A

E

T

E

D

A

K

T
x
C

E

R

A

S

-

L

-

Q

-

L

A

S

A

P

G

E

S

E

T

R

I

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T

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A

V

G

T

E

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Y

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K

D

R

L

L

D

D

K

S

D

G

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A

D

F

I

H

F

L

L

L

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L

D

D

V

V

R

R

E

P

Q

Q

N

V

E

E

Y

V

E

D

I

I

V
x
C

N

R

I

L

P

P

G

H

A

A

V

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L

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I

I

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K

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G

K

E

H

F

L

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E

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R

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R

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D

A

A

-

E

-

S

-

L

-

K

-

L

F

K

E

E

R

A

L

I

P

W

Q

E

D

E

K

K

R

Q

-

G

-

T

-

Q

-

E

-

G

-

A

-

V

-

P

I

I

V

Y

L

V

H

I

C
|
C

K
|
K

S
x
L

G
|
G

A
x
N

R
x
D

S

S

A

Q

E

K

A

A

L

V

A

K

A

I

V

L

K

Q

S

S

A

L

G

S

F

A

N

A

D

Q

A

E

V

L

-

D

-

P

-

L

-

T

-

V

-

R

H

D

V

V

G

V

G

G

V

L

L

M

A

A

W

W

V

A

N

A

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K

V

I

D

D

P

G

S

T

L

F

P
|
P

V

Q

Y
|
Y

158:238 (vs. 116:196, 46% identical) Interaction with NFS1
C239 (= C197) mutation to A: Impairs sulfurtransferase activity.
C316 (= C267) modified: Disulfide link with 324; mutation to A: Does not affect sulfurtransferase activity.
C324 (≠ T275) modified: Disulfide link with 316; mutation to A: Does not affect sulfurtransferase activity.
C365 (≠ V312) mutation to A: Does not affect sulfurtransferase activity.
C412 (= C344) active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; mutation to A: Abolishes sulfurtransferase activity.
K413 (= K345) mutation to R: Does not affect sulfurtransferase specificity and activity.
L414 (≠ S346) mutation to K: Does not affect sulfurtransferase specificity and activity.
G415 (= G347) mutation to A: Does not affect sulfurtransferase specificity and activity.
N416 (≠ A348) mutation to V: Does not affect sulfurtransferase specificity and activity.
D417 (≠ R349) mutation to R: Results in 470-fold increased activity.; mutation to T: Results in 90-fold increased activity.
P458 (= P384) mutation to G: Does not affect sulfurtransferase specificity and activity.
Y460 (= Y386) mutation to A: Does not affect sulfurtransferase specificity and activity.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
48% identity, 66% coverage: 13:267/386 of query aligns to 5:265/270 of D4GSF3

query
sites
D4GSF3

L

L

T

D

V

A

D

T

E

Q

V

L

R

D

R

R

Y

Y

S

S

R

R

H

H

L

I

I

I

I

M

P

D

D

E

V

V

G

G

M

P

D

E

G

G

Q

Q

K

G

R

R

L

L

K

L

N

S

A

S

K

R

V

V

L

V

V

V

G

G

A

A

G

G

L

L

G

G

S

A

P

P

A

A

L

I

L

Q

Y

Y

L

L

A

A

A

V

G

G

V

V

G

G

T

E

L

L

G

V

I

V

D

D

F

D

D

D

V

V

D

E

E

R

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

V

H

H

G

C

Q

D

S

D

D

D

V

V

D

G

R

T

P

P

K

K

A

A

E

E

S

S

A

A

R

A

D

A

S

F

I

V

K

R

E

G

V

L

N

N

P

P

N

D

V

V

E

S

V

V

I

E

L

P

H

V

Q

E

E

A

R

R

L

V

E

D

S

K

D

S

N

N

A

V

L

H

D

E

I

V

F

V

A

A

G

G

Y

S

D

D

L

V

I

V

L

V

D

D

G

A

T

S

D

D

N

N

F

F

A

P

T

T

R

R

Y

Y

L

L

V

L

N

N

D

D

A

V

C

C

V

R

L

F

L

E

D

G

K

I

P

P

Y

L

V

V

W

H

G

G

S

A

I

I

Y

Y

R

K

F

F

D

E

G

G

Q

Q

V

A

S

T

V

T

F

L

W

V

N

P

E

D

H

-

G

G

P

P

Q

C

Y

Y

R

R

D

C

L

L

Y

F

P

P

E

E

P

A

P

P

P

E

P

P

G

G

M

T

V

V

P

P

S

D

C
|
C

A

A

E

T

G

T

G

G

V

V

L

L

G

G

V

V

L

L

C

P

A

G

S

T

I

V

G

G

S

C

V

I

M

Q

V

A

N

T

E

E

A

A

I

M

K

K

L

L

I

L

T

L

G

D

I

E

G

G

D

E

P

A

L

L

V

D

G

G

R

R

L

L

I

F

F

Y

D

D

A

A

L

M

E

D

M

M

T

T

W

F

R

E

E

T

V

V

K

P

V

Y

R

R

K

T

D

N

P

P

D

D

T

C

E

-

P

P

V

V

T

C

-

G

-

E

-

G

-

V

-

D

-

S

-

I

E

E

L

D

I

I

D

D

Y

Y

E

V

A

E

F

S

C

C

C188 (= C197) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
40% identity, 63% coverage: 12:255/386 of query aligns to 3:245/249 of P12282

query
sites
P12282

E

E

L

L

T

S

V

D

D

Q

E

E

V

M

R

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

I

L

P

R

D

G

V

F

G

D

M

F

D

D

G

G

Q

Q

K

E

R

A

L

L

K

K

N

D

A

S

K

R

V

V

L

L

V

I

V

V

G

G

A

L

G

G

L

L

G

G

S
x
C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

D

D

F

F

D

D

V

T

V

V

D

S

E

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

I

T

I

L

H

H

G

S

Q

D

S

A

D

T

V

V

D

G

R

Q

P

P

K

K

A

V

E

E

S

S

A

A

R

R

D

D

S

A

I

L

K

T

E

R

V

I

N

N

P

P

N

H

V

I

E

A

V

I

I

T

L

P

H

V

Q

N

E

A

R

L

L

L

E

D

S

D

D

A

N

E

A

L

L

A

D

A

I

L

F

I

A

A

G

E

Y

H

D

D

L

L

I

V

L

L

D

D

G
x
C

T

T

D
|
D

N

N

F

V

A

A

T

V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C
|
C

V

F

L

A

D

K

K

V

P

P

Y

L

V

V

W

S

G

G

S

A

I

A

Y

I

R

R

F

M

D

E

G

G

Q

Q

V

I

S

T

V

V

F

F

W

T

N

Y

E

Q

H

D

G

G

-

E

P

P

Q
x
C

Y

Y

R

R

D
x
C

L

L

Y

S

P

R

E

L

P

F

P

G

P

E

P

N

G

A

M

L

V

-

P

-

S

T

C
|
C

A

V

E

E

G

A

G

G

V

V

L

M

G

A

V

P

L

L

C

I

A

G

S

V

I

I

G

G

S

S

V

L

M

Q

V

A

N

M

E

E

A

A

I

I

K

K

L

M

I

L

T

A

G

G

I

Y

G

G

D

K

P

P

L

A

V

S

G

G

R

K

L

I

L

V

I

M

F

Y

D

D

A

A

L

M

E

T

M
x
C

T

Q

W

F

R

R

E

E

V

M

K

K

V

L

R

M

K

R

D

N

P

P

D

G

T
x
C

E

E

R14 (= R23) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ S53) mutation to A: No effect.
R73 (= R82) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G137) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D139) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C151) mutation to A: No effect.
C172 (≠ Q180) mutation to A: No zinc bound and no enzyme activity.
C175 (≠ D183) mutation to A: No zinc bound and no enzyme activity.
C187 (= C197) mutation to A: No effect.
C231 (≠ M241) mutation to A: No effect.
C244 (≠ T254) mutation to A: No zinc bound and almost no enzyme activity.

Sites not aligning to the query:

247 C→A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
40% identity, 63% coverage: 12:255/386 of query aligns to 2:237/240 of 1jwbB

query
sites
1jwbB

E

E

L

L

T

S

V

D

D

Q

E

E

V

M

R

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

I

L

P

R

D

G

V

F

G

D

M

F

D

D

G

G

Q

Q

K

E

R

A

L

L

K

K

N

D

A

S

K

R

V

V

L

L

V

I

V

V

G
|
G

A

L

G
|
G

L

L

G

G

S

C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

D
|
D

F
|
F

D

D

V

T

V

V

D

S

E

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

T

I

L

H

H

G

S

Q

D

S

A

D

T

V

V

D

G

R

Q

P

P

K
|
K

A

V

E

E

S

S

A

A

R

R

D

D

S

A

I

L

K

T

E

R

V

I

N

N

P

P

N

H

V

I

E

A

V

I

I

T

L

P

H

V

Q

N

E

A

R
x
L

L

L

E

D

S

D

D

A

N

E

A

L

L

A

D

A

I

L

F

I

A

A

G

E

Y

H

D

D

L

L

I

V

L

L

D

D

G
x
C

T
|
T

D
|
D

N
|
N

F

V

A

A

T
x
V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C

C

V

F

L

A

D

K

K

V

P

P

Y

L

V

V

W

S

G

G

S

A

I

A

Y

I

R

R

F

M

D

E

G

G

Q

Q

V

I

S

T

V

V

F

F

W

T

N

Y

E

Q

H

D

G

G

-

E

P

P

Q
x
C

Y

Y

R

R

D

C

L

L

Y

S

P

R

E

L

P

F

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

G

E

E

G

A

G

G

V

V

L

M

G

A

V

P

L

L

C

I

A

G

S

V

I

I

G

G

S

S

V

L

M

Q

V

A

N

M

E

E

A

A

I

I

K

K

L

M

I

L

T

A

G

G

I

Y

G

G

D

K

P

P

L

A

V

S

G

G

R

K

L

I

L

V

I

M

F

Y

D

D

A

A

L

M

E

T

M

C

T

Q

W

F

R

R

E

E

V

M

K

K

V

L

R

M

K

R

D

N

P

P

D

G

T
x
C

E

E

active site: R13 (= R23), D129 (= D139)
binding adenosine monophosphate: G37 (= G47), G39 (= G49), G40 (= G50), D61 (= D71), F62 (= F72), K85 (= K95), L108 (≠ R118), C127 (≠ G137), T128 (= T138), D129 (= D139), N130 (= N140), V133 (≠ T143)
binding zinc ion: C171 (≠ Q180), C236 (≠ T254)

Sites not aligning to the query:

binding zinc ion: 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
40% identity, 63% coverage: 12:255/386 of query aligns to 2:237/240 of 1jw9B

query
sites
1jw9B

E

E

L

L

T

S

V

D

D

Q

E

E

V

M

R

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

I

L

P

R

D

G

V

F

G

D

M

F

D

D

G

G

Q

Q

K

E

R

A

L

L

K

K

N

D

A

S

K

R

V

V

L

L

V

I

V

V

G

G

A

L

G

G

L

L

G

G

S

C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

D

D

F

F

D

D

V

T

V

V

D

S

E

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

T

I

L

H

H

G

S

Q

D

S

A

D

T

V

V

D

G

R

Q

P

P

K

K

A

V

E

E

S

S

A

A

R

R

D

D

S

A

I

L

K

T

E

R

V

I

N

N

P

P

N

H

V

I

E

A

V

I

I

T

L

P

H

V

Q

N

E

A

R

L

L

L

E

D

S

D

D

A

N

E

A

L

L

A

D

A

I

L

F

I

A

A

G

E

Y

H

D

D

L

L

I

V

L

L

D

D

G

C

T

T

D
|
D

N

N

F

V

A

A

T

V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C

C

V

F

L

A

D

K

K

V

P

P

Y

L

V

V

W

S

G

G

S

A

I

A

Y

I

R

R

F

M

D

E

G

G

Q

Q

V

I

S

T

V

V

F

F

W

T

N

Y

E

Q

H

D

G

G

-

E

P

P

Q
x
C

Y

Y

R

R

D

C

L

L

Y

S

P

R

E

L

P

F

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

G

E

E

G

A

G

G

V

V

L

M

G

A

V

P

L

L

C

I

A

G

S

V

I

I

G

G

S

S

V

L

M

Q

V

A

N

M

E

E

A

A

I

I

K

K

L

M

I

L

T

A

G

G

I

Y

G

G

D

K

P

P

L

A

V

S

G

G

R

K

L

I

L

V

I

M

F

Y

D

D

A

A

L

M

E

T

M

C

T

Q

W

F

R

R

E

E

V

M

K

K

V

L

R

M

K

R

D

N

P

P

D

G

T
x
C

E

E

active site: R13 (= R23), D129 (= D139)
binding zinc ion: C171 (≠ Q180), C236 (≠ T254)

Sites not aligning to the query:

binding zinc ion: 239

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
32% identity, 96% coverage: 13:384/386 of query aligns to 6:421/423 of 6yubA

query
sites
6yubA

L

L

T

S

V

K

D

E

E

E

V

I

R

E

R

R

Y

Y

S

S

R

R

H

Q

L

M

I

I

I

V

P

P

D

G

V

M

G

G

M

K

D

E

G

G

Q

Q

K

L

R

R

L

L

K

M

N

N

A

A

K

K

V

V

L

L

V

I

G

G

A

A

G

G

L

L

G

G

S

C

P

P

A

A

L

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

G

G

I

I

I

V

D

D

F

G

D

D

V

S

V

V

D

E

E

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

I

A

H

H

G

A

Q

T

S

K

D

R

V

V

D

G

R

M

P

L

K

K

A

V

E

D

S

S

A

L

R

I

D

T

S

H

I

L

K

I

E

E

V

I

N

N

P

P

N

L

V

P

E

V

V

Y

I

V

L

P

H

Y

Q

R

E

F

R

D

L

L

E

T

S

P

D

Q

N

N

A

A

L

A

D

Q

I

I

F

I

A

K

G

P

Y

W

D

D

L

V

I

I

L

L

D

D

G

C

T

T

D

D

N

N

F

P

A

A

T

T

R

R

Y

Y

L

L

V

I

N

S

D

D

A

V

C

C

V

V

L

L

D

G

K

K

P

P

Y

L

V

V

W

S

G

A

S

A

I

S

Y

V

R

Q

F

K

D

S

G

G

Q

Q

V

L

S

I

V

V

F

L

W

N

N

C

E

P

H

P

G

T

P

P

Q

Q

-

G

-

V

-

N

-

K

-

A

-

P

-
x
C

Y

Y

R

R

D
x
C

L

C

Y

F

P

K

E

K

P

P

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

-

E

-

G

-

G

G

V

I

L

M

G

G

V

P

L

V

C

V

A

G

S

M

I

M

G

G

S

V

V

A

M

Q

V

A

N

G

E

E

A

A

I

I

K

K

-

I

L

L

I

V

T

S

G

Q

I

L

G

H

D

M

P

P

-

P

-

K

-

E

-

G

-

E

-

V

-

S

-

P

-

E

-

K

-

N

L

L

V

V

G

Q

R

P

L

T

L

L

I

L

-

I

-

Y

-

T

-

Y

-

D

-

L

-

N

-

S

-

A

-

I

-

G

-

P

-

Y

-

S

F

F

D

R

A

A

L

L

E

K

M

M

T

G

W

G

R

R

E

K

V

K

K

D

V
x
C

R

F

K

A

D
x
C

P

G

D

E

T

N

E

S

P

T

V

L

T

T

E

-

L

-

I

L

D

D

-

G

-

I

-

K

-

S

-

G

Y

N

E

P

A

N

F

Y

C

V

G

G

A

N

V

M

S

T

E

-

E

Q

A

S

T

T

E

N

A

L

A

A

A

P

G

E

S

D

T

R

I

I

T

T

A

A

G

T

E

A

L

Y

K

N

D

E

L

K

L

R

D

R

K

N

D

G

E

E

-

L

-

G

D

E

I

H

F

I

L

L

V

L

D

D

V

T

R

R

E

E

Q

K

N

E

E

H

Y

F

E

S

I

F

V

G

N

S

I

I

P

P

G

G

A

A

V

V

L

N

I

V

P

P

K

F

G

S

E

K

F

F

L

L

-

V

N

K

G

A

K

S

A

S

F

I

E

K

R

R

-

P

-

A

-

E

-

L

L

L

P

P

Q

M

-

Q

-

P

-

A

-

S

-

D

D

E

K

A

R

P

I

I

V

V

L

V

H

V

C

C

K

R

S

R

G

G

A

Q

R

D

S

S

A

Q

E

E

A

V

L

V

A

E

A

K

V

L

K

K

S

E

A

L

G

G

F

L

N

D

D

N

A

G

-

G

-

K

-

R

-

K

-

I

V

M

H

D

V

I

G

V

G

G

V

M

L

K

A

A

W

W

V

R

N

D

Q

E

V

V

D

D

P

P

S

D

L

F

P

P

binding zinc ion: C183 (vs. gap), C186 (≠ D183), C266 (≠ V248), C269 (≠ D251)

1zfnA Structural analysis of escherichia coli thif (see paper)
42% identity, 60% coverage: 20:250/386 of query aligns to 8:236/244 of 1zfnA

query
sites
1zfnA

R

R

Y

Y

S

S

R
|
R

H

Q

L

I

I

L

P

D

D

D

V

I

G

A

M

L

D

D

G

G

Q

Q

K

Q

R

K

L

L

K

L

N

D

A

S

K

Q

V

V

L

L

V

I

V
x
I

G
|
G

A

L

G
|
G

L

L

G

G

S

T

P

P

A

A

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

I

A

D
|
D

F

D

D

D

V

D

V

V

D

H

E

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

I

I

I

L

H

F

G

T

Q

T

S

E

D

D

V

I

D

D

R

R

P

P

K
|
K

A

S

E

Q

S

V

A

S

R

Q

D

Q

S

R

I

L

K

T

E

Q

V

L

N

N

P

P

N

D

V

I

E

Q

V

L

I

T

L

A

H

L

Q

Q

E

Q

R

R

L

L

E

T

S

G

D

E

N

A

A

L

L

K

D

D

I

A

F

V

A

A

G

R

Y

A

D

D

L

V

I

V

L

L

D

D

G

C

T
|
T

D
|
D

N

N

F

M

A

A

T
|
T

R

R

Y

Q

L

E

V

I

N

N

D

A

A

A

C

C

V

V

L

A

L

L

D

N

K

T

P

P

Y

L

V

I

W

T

G

A

S

S

I

A

Y

V

R

G

F

F

D

G

G

G

Q

Q

V

L

S

M

V

V

F

L

-

T

-

P

W

W

N

-

E

E

H

Q

G

G

P
x
C

Q

-

Y

Y

R

R

D
x
C

L

L

Y

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

M

R

V

-

P

-

S

N

C

C

A

R

E

T

G

A

G

G

V

V

L

V

G

G

V

P

L

V

C

V

A

G

S

V

I

M

G

G

S

T

V

L

M

Q

V

A

N

L

E

E

A

A

I

I

K

K

L

L

I

L

T

S

G

G

I

I

G

E

D

T

P

P

L

-

V

A

G

G

R

E

L

L

L

R

I

L

F

F

D

D

A

G

L

K

E

S

M

S

T

Q

W

W

R

R

E

S

V

L

K

A

V

L

R

R

K

R

active site: R11 (= R23), D127 (= D139)
binding adenosine-5'-triphosphate: I34 (≠ V46), G35 (= G47), G37 (= G49), G38 (= G50), D59 (= D71), R70 (= R82), Q71 (= Q83), K83 (= K95), T126 (= T138), D127 (= D139), T131 (= T143)
binding zinc ion: C169 (≠ P179), C172 (≠ D183)

Sites not aligning to the query:

binding zinc ion: 240, 243

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 60% coverage: 20:250/386 of query aligns to 8:236/251 of P30138

query
sites
P30138

R

R

Y

Y

S

S

R

R

H

Q

L

I

I

L

P

D

D

D

V

I

G

A

M

L

D

D

G

G

Q

Q

K

Q

R

K

L

L

K

L

N

D

A

S

K

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

S

T

P

P

A

A

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

I

A

D

D

F

D

D

D

V

D

V

V

D

H

E

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

I

L

H

F

G

T

Q

T

S

E

D

D

V

I

D

D

R

R

P

P

K

K

A

S

E

Q

S

V

A

S

R

Q

D

Q

S

R

I

L

K

T

E

Q

V

L

N

N

P

P

N

D

V

I

E

Q

V

L

I

T

L

A

H

L

Q

Q

E

Q

R

R

L

L

E

T

S

G

D

E

N

A

A

L

L

K

D

D

I

A

F

V

A

A

G

R

Y

A

D

D

L

V

I

V

L

L

D

D

G

C

T

T

D

D

N

N

F

M

A

A

T

T

R

R

Y

Q

L

E

V

I

N

N

D

A

A

A

C

C

V

V

L

A

L

L

D

N

K

T

P

P

Y

L

V

I

W

T

G

A

S

S

I

A

Y

V

R

G

F

F

D

G

G

G

Q

Q

V

L

S

M

V

V

F

L

-

T

-

P

W

W

N

-

E

E

H

Q

G

G

P
x
C

Q

-

Y

Y

R

R

D
x
C

L

L

Y
x
W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

M

R

V

-

P

-

S

N

C
|
C

A

R

E

T

G

A

G

G

V

V

L

V

G

G

V

P

L

V

C

V

A

G

S

V

I

M

G

G

S

T

V

L

M

Q

V

A

N

L

E

E

A

A

I

I

K

K

L

L

I

L

T

S

G

G

I

I

G

E

D

T

P

P

L

-

V

A

G

G

R

E

L

L

L

R

I

L

F

F

D

D

A

G

L

K

E

S

M

S

T

Q

W

W

R

R

E

S

V

L

K

A

V

L

R

R

K

R

C169 (≠ P179) binding Zn(2+)
C172 (≠ D183) binding Zn(2+)
W174 (≠ Y185) mutation to A: No adenylation of ThiS.
C184 (= C197) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.

Sites not aligning to the query:

240 binding Zn(2+)
243 binding Zn(2+)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
38% identity, 62% coverage: 12:250/386 of query aligns to 2:217/217 of 1jwaB

query
sites
1jwaB

E

E

L

L

T

S

V

D

D

Q

E

E

V

M

R

L

R

R

Y

Y

S

N

R
|
R

H

Q

L

I

I

I

I

L

P

R

D

G

V

F

G

D

M

F

D

D

G

G

Q

Q

K

E

R

A

L

L

K

K

N

D

A

S

K

R

V

V

L

L

V

I

V

V

G

G

A

L

G
|
G

L

L

G

G

S

C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

D
|
D

F
|
F

D

D

V

T

V

V

D

S

E

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

I

T

I

L

H

H

G

S

Q

D

S

A

D

T

V

V

D

G

R

Q

P

P

K
|
K

A

V

E

E

S

S

A

A

R

R

D

D

S

A

I

L

K

T

E

R

V

I

N

N

P

P

N

H

V

I

E

A

V

I

I

T

L

P

H

V

Q

N

E

A

R
x
L

L

L

E

D

S

D

D

A

N

E

A

L

L

A

D

A

I

L

F

I

A

A

G

E

Y

H

D

D

L

L

I

V

L

L

D

D

G

C

T

T

D
|
D

N
|
N

F

V

A

A

T
x
V

R

R

Y

N

L

Q

V

L

N

N

D

A

A

G

C

C

V

F

L

A

D

K

K

V

P

P

Y

L

V

V

W

S

G

G

S

A

I

A

Y

I

R

R

F

M

D

E

G

G

Q

Q

V

I

S

T

V

V

F

F

W

T

N

Y

E

-

H

-

G

-

P

-

Q

-

Y

-

R

-

D

-

L

-

Y

-

P

-

E

-

P

-

G

-

M

-

V

-

P

-

S

-

C

-

A

-

E

E

G

A

G

G

V

V

L

M

G

A

V

P

L

L

C

I

A

G

S

V

I

I

G

G

S

S

V

L

M

Q

V

A

N

M

E

E

A

A

I

I

K

K

L

M

I

L

T

A

G

G

I

Y

G

G

D

K

P

P

L

A

V

S

G

G

R

K

L

I

L

V

I

M

F

Y

D

D

A

A

L

M

E

T

M

C

T

Q

W

F

R

R

E

E

V

M

K

K

V

L

R

M

K

R

active site: R13 (= R23), D129 (= D139)
binding adenosine-5'-triphosphate: G39 (= G49), G40 (= G50), D61 (= D71), F62 (= F72), R72 (= R82), K85 (= K95), L108 (≠ R118), D129 (= D139), N130 (= N140), V133 (≠ T143)

1zud3 Structure of this-thif protein complex (see paper)
42% identity, 60% coverage: 20:250/386 of query aligns to 8:231/240 of 1zud3

query
sites
1zud3

R

R

Y

Y

S

S

R
|
R

H

Q

L

I

I
x
L

I

L

P
x
D

D

D

V

I

G

A

M

L

D

D

G

G

Q

Q

K

Q

R

K

L

L

K

L

N

D

A

S

K

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

S

T

P

P

A

A

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

I

A

D

D

F

D

D

D

V

D

V

V

D

H

E

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

I

I

I

L

H

F

G

T

Q

T

S

E

D

D

V

I

D

D

R

R

P

P

K

K

A

S

E

Q

S

V

A

S

R

Q

D

Q

S

R

I

L

K

T

E

Q

V

L

N

N

P

P

N

D

V

I

E

Q

V

L

I

T

L

A

H

L

Q

Q

E

Q

R

R

L

L

E

T

S

G

D

E

N

A

A

L

L

K

D

D

I

A

F

V

A

A

G

R

Y

A

D

D

L

V

I

V

L

L

D

D

G

C

T

T

D
|
D

N

N

F

M

A

A

T

T

R

R

Y

Q

L

E

V

I

N

N

D

A

A

A

C

C

V

V

L

A

L

L

D

N

K

T

P

P

Y

L

V

I

W

T

G

A

S

S

I

A

Y

V

R

G

F

F

D

G

G

G

Q

Q

V

L

S

M

V

V

F

L

-

T

-

P

W

W

N

-

E

E

H

Q

G

G

P
x
C

Q

-

Y

Y

R

R

D

C

L

L

Y

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

T

M

A

V

G

P

V

S

V

C

G

A

P

E

V

G

V

G

G

V

V

L

M

G

G

V

T

L

L

C

Q

A

A

S

L

I

-

G

-

S

-

V

-

M

-

V

-

N

-

E

E

A

A

I

I

K

K

L

L

I

L

T

S

G

G

I

I

G

E

D

T

P

P

L

-

V

A

G

G

R

E

L

L

L

R

I

L

F

F

D

D

A

G

L

K

E

S

M

S

T

Q

W

W

R

R

E

S

V

L

K

A

V

L

R

R

K

R

active site: R11 (= R23), D127 (= D139)
binding calcium ion: L14 (≠ I26), D16 (≠ P28)
binding zinc ion: C169 (≠ P179)

Sites not aligning to the query:

binding zinc ion: 235, 238

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
42% identity, 55% coverage: 11:222/386 of query aligns to 5:212/289 of 6yubB

query
sites
6yubB

D

K

E

S

L

L

T

S

V

K

D

E

E

E

V

I

R

E

R

R

Y

Y

S

S

R

R

H

Q

L

M

I

I

I

V

P

P

D

G

V

M

G

G

M

K

D

E

G

G

Q

Q

K

L

R

R

L

L

K

M

N

N

A

A

K

K

V

V

L

L

V

I

G

G

A

A

G

G

L

L

G

G

S

C

P

P

A

A

L

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

G

G

I

I

I

V

D

D

F

G

D

D

V

S

V

V

D

E

E

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

I

V

I

A

H

H

G

A

Q

T

S

K

D

R

V

V

D

G

R

M

P

L

K

K

A

V

E

D

S

S

A

L

R

I

D

T

S

H

I

L

K

I

E

E

V

I

N

N

P

P

N

L

V

P

E

V

V

Y

I

V

L

P

H

Y

Q

R

E

F

R

D

L

L

E

T

S

P

D

Q

N

N

A

A

L

A

D

Q

I

I

F

I

A

K

G

P

Y

W

D

D

L

V

I

I

L

L

D

D

G

C

T

T

D

D

N

N

F

P

A

A

T

T

R

R

Y

Y

L

L

V

I

N

S

D

D

A

V

C

C

V

V

L

L

D

G

K

K

P

P

Y

L

V

V

W

S

G

A

S

A

I

S

Y

V

R

Q

F

K

D

S

G

G

Q

Q

V

L

S

I

V

V

F

-

W

-

N

-

E

-

H

-

G

-

P

-

Q

-

Y

-

R

-

D

-

L

L

Y

N

P

C

E

P

P

P

P

T

P

P

P

Q

G

G

M

V

V

V

P

N

S

K

-

K

-

A

-

P

-
x
C

-

Y

-

R

C
|
C

A

C

E

F

G

K

G

G

V

I

L

M

G

G

V

P

L

V

C

V

A

G

S

M

I

M

G

G

S

V

V

A

M

Q

V

A

N

G

E

E

A

A

I

I

K

K

L

I

I

L

binding zinc ion: C184 (vs. gap), C187 (= C197)

Sites not aligning to the query:

binding zinc ion: 265

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 44% coverage: 28:198/386 of query aligns to 13:182/628 of O42939

query
sites
O42939

P

P

D

L

V

T

G

F

M

V

D

E

G

A

Q

L

K

R

R

N

L

F

K

K

N

S

A

A

K

K

V

V

L

L

V

L

V

V

G

G

A

A

G

G

L

I

G

G

S

C

P

E

A

L

L

L

L

K

Y

N

L

L

A

L

A

M

A

S

G

G

V

V

G

K

T

E

L

V

G

H

I

I

D

D

F

L

D

D

V

T

V

I

D

D

E

L

S

S

N

N

L

L

Q

N

R

R

Q

Q

I

F

I

L

H

F

G

R

Q

K

S

K

D

H

V

V

D

K

R

Q

P

P

K

K

A

A

E

I

S

V

A

A

R

A

D

K

S

T

I

A

K

S

E

S

V

F

N

N

P

P

N

N

V

V

E

K

V

L

I

E

L

A

H

Y

Q

H

E

A

R

N

L

I

E

K

S

E

D

D

N

R

-

F

A

N

L

V

D

A

I

W

F

F

A

R

G

Q

Y

F

D

D

L

L

I

V

L

F

D

N

G

A

T

L

D

D

N

N

F

L

A

D

T

A

R

R

Y

R

L

H

V

V

N

N

D

K

A

Q

C

C

V

L

L

L

L

A

D

S

K

V

P

P

Y

L

V

I

W

E

G

S

S

G

I

T

Y

T

R

G

F

F

D

L

G

G

Q

Q

V

V

S

Q

V

V

F

I

W

I

N

H

E

G

H

K

G

T

P

E

Q

C

Y

Y

R

-

D

D

L

C

Y

N

P

P

E

K

P

-

P

E

P

P

G

K

M

T

V

Y

P

P

S

V

C

C

A

T

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

6xogB Structure of sumo1-ml786519 adduct bound to sae (see paper)
32% identity, 39% coverage: 42:192/386 of query aligns to 12:162/500 of 6xogB

query
sites
6xogB

K

R

V

V

L

L

V

V

G

G

A

A

G

G

L

I

G

G

S

C

P

E

A

L

L

L

L

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

S

T

H

L

I

G

D

I

L

I

I

D
|
D

F
x
L

D

D

V

T

V

I

D

D

E

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

I

F

I

L

H

F

G

Q

Q

K

S

K

D

H

V

V

D

G

R

R

P

S

K
|
K

A

A

E

Q

S

V

A

A

R

K

D

E

S

S

I

V

K

L

E

Q

V

F

N

Y

P

P

N

K

V

A

E

N

V

I

I

V

-

A

L

Y

H

H

Q

D

E
x
S

R
x
I

L
x
M

E

N

S

P

D

D

N

Y

A

N

L

V

D

E

I

F

F

F

A

R

G

Q

Y

F

D

I

L

L

I

V

L

M

D

N

G
x
A

T

L

D
|
D

N
|
N

F

R

A

A

T

A

R

R

Y

N

L

H

V

V

N

N

D

R

A

M

C

C

V

L

L

A

D

D

K

V

P

P

Y

L

V

I

W

E

G

S

S

G

I

T

Y

A

R

G

F

Y

D

L

G

G

Q

Q

V

V

S

T

V

T

F

I

W

K

N

K

E

G

H

V

G

T

P

E

Q
x
C

Y

Y

R

-

D

E

L
x
C

Y

H

P

P

E

K

P

P

P

T

P

F

P

P

G

G

active site: D110 (= D139)
binding {(1R,2R,3S,4R)-4-[(5-{4-[(1S)-1-(6-bromopyridin-2-yl)-1-hydroxyethyl]thiophene-2-carbonyl}pyrimidin-4-yl)amino]-2,3-dihydroxycyclopentyl}methyl sulfamate: D41 (= D71), L42 (≠ F72), K65 (= K95), S88 (≠ E117), I89 (≠ R118), M90 (≠ L119), A108 (≠ G137), D110 (= D139), N111 (= N140)
binding zinc ion: C151 (≠ Q180), C154 (≠ L184)

Sites not aligning to the query:

active site: 163, 164, 166
binding zinc ion: 398, 401

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
31% identity, 42% coverage: 38:198/386 of query aligns to 16:175/650 of Q7SXG4

query
sites
Q7SXG4

L

L

K

S

N

S

A

C

K

R

V

V

L

L

V

V

G

G

A

A

G

G

L

I

G

G

S

C

P

E

A

L

L

L

L

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

K

T

N

L

I

G

E

I

V

I

I

D

D

F

L

D

D

V

T

V

I

D

D

E

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

I

F

I

L

H

F

G

Q

Q

K

S

K

D

H

V

V

D

G

R

K

P

S

K

K

A

A

E

Q

S

V

A

A

R

K

D

E

S

S

I

V

K

L

E

R

V

F

N

C

P

P

N

S

V

A

E

N

V

I

I

T

-

A

L

Y

H

H

Q

D

E

S

R

I

L

M

E

N

S

P

D

D

N

Y

A

N

L

V

D

E

I

F

F

F

A

R

G

N

Y

F

D

Q

L

L

I

V

L

M

D

N

G

A

T

L

D

D

N

N

F

R

A

A

T

A

R

R

Y

N

L

H

V

V

N

N

D

R

A

M

C

C

V

L

L

A

D

D

K

I

P

P

Y

L

V

I

W

E

G

S

S

G

I

T

Y

A

R

G

F

Y

D

L

G

G

Q

Q

V

V

S

T

V

V

F

I

W

K

N

K

E

G

H

Q

G

T

P

E

Q

C

Y

Y

R

-

D

E

L

C

Y

Q

P

P

E

K

P

-

P

P

P

T

P

Q

G

K

M

T

V

F

P

P

S

G

C

C

A

T

Sites not aligning to the query:

599 modified: Phosphoserine

3kydB Human sumo e1~sumo1-amp tetrahedral intermediate mimic (see paper)
27% identity, 53% coverage: 42:247/386 of query aligns to 16:232/477 of 3kydB

query
sites
3kydB

K

R

V

V

L

L

V

V

G

G

A

A

G

G

L
x
I

G

G

S

C

P

E

A

L

L

L

L

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

S

T

H

L

I

G

D

I

L

I

I

D
|
D

F
x
L

D

D

V

T

V

I

D

D

E

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

I

F

I

L

H

F

G

Q

Q

K

S

K

D

H

V

V

D

G

R

R

P

S

K
|
K

A

A

E

Q

S

V

A

A

R

K

D

E

S

S

I

V

K

L

E

Q

V

F

N

Y

P

P

N

K

V

A

E

N

V

I

I

V

-

A

L

Y

H

H

Q

D

E
x
S

R
x
I

L

M

E

N

S

P

D

D

N

Y

A

N

L

V

D

E

I

F

F

F

A

R

G

Q

Y

F

D

I

L

L

I

V

L

M

D

N

G

A

T
x
L

D
|
D

N
|
N

F

R

A

A

T

A

R

R

Y

N

L

H

V

V

N

N

D

R

A

M

C

C

V

L

L

A

D

D

K

V

P

P

Y

L

V

I

W

E

G

S

S

G

I

T

Y

A

R

G

F

Y

D

L

G

G

Q

Q

V

V

S

T

V

T

F

I

W

K

N

K

E

G

H

V

G

T

P

E

Q
x
C

Y

Y

R

-

D

E

L
x
C

Y

H

P

P

E

K

P

P

P

T

P

Q

P

R

G

T

M

F

V

-

P

P

S

G

C
|
C

A
x
T

E

I

G
x
R

G

N

V

T

L

P

G

S

-

E

-

P

V

I

L

H

C

C

A

I

S

V

I

W

G

A

S

K

V

Y

M

L

V

F

N

N

E

Q

A

L

-

F

-

T

-

K

-

E

-

W

-

A

-

K

-

S

-

T

-

G

-

Y

-

D

-

P

I

V

K

K

L

L

I

F

T

T

G

K

I

L

G

F

D

K

P

D

L

D

V

I

G

R

R

Y

L

L

I

T

F

M

D

D

A

K

L

L

E

-

M

-

T

-

W

W

R

R

E

K

V

R

K

K

active site: D114 (= D139), C170 (= C197), T171 (≠ A198), R173 (≠ G200)
binding 5'-{[(3-aminopropyl)sulfonyl]amino}-5'-deoxyadenosine: I25 (≠ L51), D45 (= D71), L46 (≠ F72), K69 (= K95), S92 (≠ E117), I93 (≠ R118), L113 (≠ T138), D114 (= D139), N115 (= N140), C170 (= C197), R173 (≠ G200)
binding zinc ion: C155 (≠ Q180), C158 (≠ L184)

Sites not aligning to the query:

binding zinc ion: 370, 373

3kycB Human sumo e1 complex with a sumo1-amp mimic (see paper)
31% identity, 41% coverage: 42:198/386 of query aligns to 15:170/548 of 3kycB

query
sites
3kycB

K

R

V

V

L

L

V

V

G

G

A

A

G
|
G

L

I

G

G

S

C

P

E

A

L

L

L

L

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

S

T

H

L

I

G

D

I

L

I

I

D
|
D

F
x
L

D

D

V

T

V

I

D

D

E

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

I

F

I

L

H

F

G

Q

Q

K

S

K

D

H

V

V

D

G

R

R

P

S

K
|
K

A

A

E

Q

S

V

A

A

R

K

D

E

S

S

I

V

K

L

E

Q

V

F

N

Y

P

P

N

K

V

A

E

N

V

I

I

V

-

A

L

Y

H

H

Q

D

E
x
S

R
x
I

L

M

E

N

S

P

D

D

N

Y

A

N

L

V

D

E

I

F

F

F

A

R

G

Q

Y

F

D

I

L

L

I

V

L

M

D

N

G

A

T
x
L

D
|
D

N
|
N

F

R

A

A

T

A

R

R

Y

N

L

H

V

V

N

N

D

R

A

M

C

C

V

L

L

A

D

D

K

V

P

P

Y

L

V

I

W

E

G

S

S

G

I

T

Y

A

R

G

F

Y

D

L

G

G

Q

Q

V

V

S

T

V

T

F

I

W

K

N

K

E

G

H

V

G

T

P

E

Q
x
C

Y

Y

R

-

D

E

L
x
C

Y

H

P

P

E

K

P

P

P

T

P

Q

P

R

G

T

M

F

V

-

P

P

S

G

C
|
C

A
x
T

active site: D113 (= D139), C169 (= C197), T170 (≠ A198)
binding 5'-deoxy-5'-(sulfamoylamino)adenosine: G22 (= G49), G23 (= G50), D44 (= D71), L45 (≠ F72), K68 (= K95), S91 (≠ E117), I92 (≠ R118), L112 (≠ T138), D113 (= D139), N114 (= N140)
binding zinc ion: C154 (≠ Q180), C157 (≠ L184)

Sites not aligning to the query:

active site: 172
binding zinc ion: 407, 410

Query Sequence

>WP_017596912.1 NCBI__GCF_000341125.1:WP_017596912.1
MSLPPLVEPADELTVDEVRRYSRHLIIPDVGMDGQKRLKNAKVLVVGAGGLGSPALLYLA
AAGVGTLGIIDFDVVDESNLQRQIIHGQSDVDRPKAESARDSIKEVNPNVEVILHQERLE
SDNALDIFAGYDLILDGTDNFATRYLVNDACVLLDKPYVWGSIYRFDGQVSVFWNEHGPQ
YRDLYPEPPPPGMVPSCAEGGVLGVLCASIGSVMVNEAIKLITGIGDPLVGRLLIFDALE
MTWREVKVRKDPDTEPVTELIDYEAFCGAVSEEATEAAAGSTITAGELKDLLDKDEDIFL
VDVREQNEYEIVNIPGAVLIPKGEFLNGKAFERLPQDKRIVLHCKSGARSAEALAAVKSA
GFNDAVHVGGGVLAWVNQVDPSLPVY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory