SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017598059.1 NCBI__GCF_000341125.1:WP_017598059.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP51 Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
65% identity, 100% coverage: 1:454/455 of query aligns to 1:458/464 of P9WP51

query
sites
P9WP51

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K428 (≠ R424) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7xoyA Cystathionine beta-synthase of mycobacterium tuberculosis in the presence of s-adenosylmethionine and serine. (see paper)
65% identity, 99% coverage: 3:454/455 of query aligns to 1:456/458 of 7xoyA

query
sites
7xoyA

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binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: K42 (= K44), S70 (= S72), N72 (= N74), T73 (= T75), T180 (= T182), G182 (= G184), T183 (= T185), S267 (= S269), P293 (= P295)

7xnzB Native cystathionine beta-synthase of mycobacterium tuberculosis. (see paper)
65% identity, 99% coverage: 3:454/455 of query aligns to 1:456/458 of 7xnzB

query
sites
7xnzB

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P

L

L

A

R

T

M

V

I

G

G

T

A

G

G

T

E

P

L

V

V

G

S

D

A

C

A

V

G

R

K

L

A

L

L

R

R

G

D

S

W

G

D

A

A

L

L

V

M

V

V

L

V

R

E

D

E

G

G

R

K

P

P

V

V

G

G

I

V

L

I

T

T

R

R

Q

Y

D

D

L

L

A

G

H

F

L

L

S

S

binding pyridoxal-5'-phosphate: K42 (= K44), G179 (= G181), T180 (= T182), G181 (= G183), G182 (= G184), T183 (= T185), S267 (= S269), P293 (= P295), D294 (= D296)

7qgtB Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
38% identity, 100% coverage: 2:455/455 of query aligns to 35:495/500 of 7qgtB

query
sites
7qgtB

R

K

V

I

H

L

D

P

S

D

L

I

I

L

D

K

L

K

V

I

G

G

D

D

T

T

P

P

L

M

V

V

R

R

L

I

R

N

K

K

V

I

T

G

E

K

-

K

-

F

G

G

L

L

A

K

P

C

T

E

I

L

L

L

A

A

K

K

V

C

E

E

Y

F

F

F

N

N

P

A

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

S

L

L

R

R

M

M

V

I

E

E

A

D

A

A

E

E

K

R

S

D

G

G

E

T

L

L

R

K

A

P

G

G

G

D

T

T

I

I

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

L

V

A

A

A

Q

A

E

V

K

R

G

G

Y

Y

R

R

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

S

D

E

K

K

L

V

A

D

V

V

L

L

R

R

A

A

Y

L

G

G

A

A

E

E

V

I

V

V

V

R

C

T

P

P

T

T

T

N

V

A

A

R

P

F

E

D

H

S

P

P

D

E

S

S

Y

H

Y

V

S

G

V

V

S

A

D

W

R

R

L

L

A

K

E

N

E

E

I

I

P

P

G

N

A

S

W

H

K

I

P

L

N

D

Q
|
Q

Y

Y

E

R

N

N

Q
x
A

N

S

N

N

P
|
P

E
x
L

S

A

H

H

Y
|
Y

H

D

S

T

T

T

G

A

P

D

E

E

I

I

W

L

D

Q

Q

Q

T

C

E

D

G

G

R

K

V

L

T

D

H

M

F

L

V

V

A

A

G

S

I

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

T

I

G

A

R
|
R

Y

K

L

L

K

K

E

E

V

K

S

C

D

P

G

G

R

-

G

C

K

R

V

I

I

I

G

G

A

V

D

D

P

P

E

E

G

G

S

S

V

I

-

L

-

A

-

E

-

P

-

E

Y

L

S

N

G

Q

G

T

S

E

G

Q

R

T

P

T

Y

Y

L

E

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

I

P

P

G

T

T

V

Y

L

D

D

T

R

G

T

I

V

C

V

D

D

D

K

I

W

V

F

A

K

V

S

S

N

D

D

K

E

E

E

S

A

F

F

L

T

M

F

T

A

R

R

R

M

L

L

A

I

R

A

E

Q

E

E

A

G

L

L

V

C

G

G

S
|
S

C

A

G

G

L

S

A

T

V

V

E

A

A

V

A

A

L

V

R

K

V

A

A

A

E

Q

D

E

A

L

G

Q

P

E

D

G

D

Q

V

R

I

C

V

V

L

I

L

L

P
|
P

D
|
D

G

S

G

V

R

R

G

N

Y

Y

L

M

G

T

K

K

I

F

F

L

N

S

D

D

E

R

W

W

M

M

A

L

D

Q

Y

K

G

G

F

F

L

L

S

K

A

E

E

E

-

D

-

L

T

T

E

E

E

K

A

K

T

P

A

W

G

W

Q

W

V

H

L

L

N

R

G

V

K

Q

G

E

G

L

E

G

M

L

P

S

D

A

F

P

V

L

H

T

T

V

H

L

P

P

H

T

E

I

S

T

I

C

G

G

T

H

A

T

V

I

A

E

I

I

M

L

R

R

E

E

Y

K

G

G

V

F

S

D

Q

Q

L

A

P

P

V

V

M

V

K

D

E

E

E

-

P

-

V

-

M

-

A

A

A

G

E

V

V

I

V

L

G

G

S

M

I

V

A

T

E

L

R

G

D

N

V

M

L

L

D

S

A

S

L

L

F

L

D

A

G

G

R

K

A

V

Q

Q

L

P

D

S

D

D

S

Q

V

V

E

G

T

K

H

V

M

I

G

Y

K

K

P

Q

L

F

A

K

T

Q

V

I

G

R

T

L

G

T

T

D

P

T

V

L

G

G

D

R

C

L

V

S

R

H

L

I

L

L

R

E

-

M

G

D

S

H

G

F

A

A

L

L

V

V

L

H

R

E

D

Q

G

Q

R

R

P

Q

V

M

-

V

-

F

G

G

I

V

L

V

T

T

R

A

Q

I

D

D

L

L

A

N

H

F

L

V

S

A

G

A

binding protoporphyrin ix containing fe: A186 (≠ Q151), P189 (= P154), L190 (≠ E155), Y193 (= Y158), R226 (= R191)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: K79 (= K44), T106 (= T71), S107 (= S72), N109 (= N74), T110 (= T75), Q182 (= Q147), G216 (= G181), T217 (= T182), G218 (= G183), T220 (= T185), G265 (= G225), S309 (= S269), P335 (= P295), D336 (= D296)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 12, 13, 14, 23, 24, 25

8s5hA Full-length human cystathionine beta-synthase with c-terminal 6xhis- tag, basal state, helical reconstruction (see paper)
40% identity, 88% coverage: 2:402/455 of query aligns to 34:438/507 of 8s5hA

query
sites
8s5hA

R

K

V

I

H

L

D

P

S

D

L

I

I

L

D

K

L

K

V

I

G

G

D

D

T

T

P

P

L

M

V

V

R

R

L

I

R

N

K

K

V

I

T

G

E

K

-

K

-

F

G

G

L

L

A

K

P

C

T

E

I

L

L

L

A

A

K

K

V

C

E

E

Y

F

F

F

N

N

P

A

G

G

S

S

V

V

K

K

D

D

R

R

I

I

A

S

L

L

R

R

M

M

V

I

E

E

A

D

A

A

E

E

K

R

S

D

G

G

E

T

L

L

R

K

A

P

G

G

G

D

T

T

I

I

V

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

M

L

V

A

A

A

Q

A

E

V

K

R

G

G

Y

Y

R

R

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

S

D

E

K

K

L

V

A

D

V

V

L

L

R

R

A

A

Y

L

G

G

A

A

E

E

V

I

V

V

V

R

C

T

P

P

T

T

T

N

V

A

A

R

P

F

E

D

H

S

P

P

D

E

S

S

Y

H

Y

V

S

G

V

V

S

A

D

W

R

R

L

L

A

K

E

N

E

E

I

I

P

P

G

N

A

S

W

H

K

I

P

L

N

D

Q

Q

Y

Y

E

R

N

N

Q
x
A

N

S

N

N

P

P

E

L

S

A

H

H

Y
|
Y

H

D

S

T

T

T

G

A

P

D

E

E

I

I

W

L

D

Q

Q

Q

T

C

E

D

G

G

R

K

V

L

T

D

H

M

F

L

V

V

A

A

G

S

I

V

G

G

T

T

G

G

T

T

I

I

S

T

G

G

T

I

G

A

R
|
R

Y

K

L

L

K

K

E

E

V

K

S

C

D

P

G

G

R

-

G

C

K

R

V

I

I

I

G

G

A

V

D

D

P

P

E

E

G

G

S

S

V

I

-

L

-

A

-

E

-

P

-

E

Y

L

S

N

G

Q

G

T

S

E

G

Q

R

T

P

T

Y

Y

L

E

V

V

E

E

G

G

V

I

G

G

E

Y

D

D

I

F

W

I

P

P

G

T

T

V

Y

L

D

D

T

R

G

T

I

V

C

V

D

D

D

K

I

W

V

F

A

K

V

S

S

N

D

D

K

E

E

E

S

A

F

F

L

T

M

F

T

A

R

R

R

M

L

L

A

I

R

A

E

Q

E

E

A

G

L

L

V

C

G

G

S

S

C

A

G

G

L

S

A

T

V

V

E

A

A

V

A

A

L

V

R

K

V

A

A

A

E

Q

D

E

A

L

G

Q

P

E

D

G

D

Q

V

R

I

C

V

V

L

I

L

L

P

P

D

D

G

S

G

V

R

R

G

N

Y

Y

L

M

G

T

K

K

I

F

F

L

N

S

D

D

E

R

W

W

M

M

A

L

D

Q

Y

K

G

G

F

F

L

L

S

K

A

E

E

E

-

D

-

L

T

T

E

E

E

K

A

K

T

P

A

W

G

W

Q

W

V

H

L

L

N

R

G

V

K

Q

G

E

G

L

E

G

M

L

P

S

D

A

F

P

V

L

H

T

T

V

H

L

P

P

H

T

E

I

S

T

I

C

G

G

T

H

A

T

V

I

A

E

I

I

M

L

R

R

E

E

Y

K

G

G

V

F

S

D

Q

Q

L

A

P

P

V

V

M

V

K

D

E

E

E

-

P

-

V

-

M

-

A

A

A

G

E

V

V

I

V

L

G

G

S

M

I

V

A

T

E

L

R

G

D

N

V

M

L

L

D

S

A

S

L

L

F

L

D

A

G

G

R

K

A

V

Q

Q

L

P

D

S

D

D

S

Q

V

V

binding protoporphyrin ix containing fe: A185 (≠ Q151), Y192 (= Y158), R225 (= R191)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 17, 22, 23, 24

4pcuA Crystal structure of delta516-525 e201s human cystathionine beta- synthase with adomet (see paper)
37% identity, 100% coverage: 2:455/455 of query aligns to 33:486/486 of 4pcuA

query
sites
4pcuA

R

K

V

I

H

L

D

P

S

D

L

I

I

L

D

K

L

K

V

I

G

G

D

D

T

T

P

P

L

M

V

V

R

R

L

I

R

N

K

K

V

I

T

G

E

K

-

K

-

F

G

G

L

L

A

K

P

C

T

E

I

L

L

L

A

A

K

K

V

C

E

E

Y

F

F

F

N

N

P

A

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

S

L

L

R

R

M

M

V

I

E

E

A

D

A

A

E

E

K

R

S

D

G

G

E

T

L

L

R

K

A

P

G

G

G

D

T

T

I

I

V

I

E

E

P

P

T

T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

L

V

A

A

A

Q

A

E

V

K

R

G

G

Y

Y

R

R

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

S

D

E

K

K

L

V

A

D

V

V

L

L

R

R

A

A

Y

L

G

G

A

A

E

E

V

I

V

V

V

R

C

T

P

P

T

A

T

R

V

F

A

-

P

-

E

D

H

S

P

P

D

S

S

S

Y

H

Y

V

S

G

V

V

S

A

D

W

R

R

L

L

A

K

E

N

E

E

I

I

P

P

G

N

A

S

W

H

K

I

P

L

N

D

Q

Q

Y

Y

E

R

N

N

Q
x
A

N

S

N

N

P
|
P

E
x
L

S

A

H

H

Y
|
Y

H

D

S

T

T

T

G

A

P

D

E

E

I

I

W

L

D

Q

Q

Q

T

C

E

D

G

G

R

K

V

L

T

D

H

M

F

L

V

V

A

A

G

S

I

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

T

I

G

A

R
|
R

Y

K

L

L

K

K

E

E

V

K

S

C

D

P

G

G

R

-

G

C

K

R

V

I

I

I

G

G

A

V

D
|
D

P

P

E

E

G

G

S

S

V

I

-

L

-

A

-

E

-

P

-

E

Y

L

S

N

G

Q

G

T

S

E

G

Q

R

T

P

T

Y

Y

L

E

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

I

P

P

G
x
T

T

V

Y

L

D

D

T

R

G

T

I

V

C

V

D

D

D

K

I

W

V

F

A

K

V

S

S

N

D

D

K

E

E

E

S

A

F

F

L

T

M

F

T

A

R

R

R

M

L

L

A

I

R

A

E

Q

E

E

A

G

L

L

V

C

G

G

S
|
S

C

A

G

G

L

S

A

T

V

V

E

A

A

V

A

A

L

V

R

K

V

A

A

A

E

Q

D

E

A

L

G

Q

P

E

D

G

D

Q

V

R

I

C

V

V

L

I

L

L

P
|
P

D
|
D

G

S

G

V

R

R

G

N

Y

Y

L

M

G

T

K

K

I

F

F

L

N

S

D

D

E

R

W

W

M

M

A

L

D

Q

Y

K

G

G

F

F

L

L

S

K

A

E

E

E

T

D

E

L

E

K

A

K

T

P

A

W

G

W

Q

W

V

H

L

L

N

R

G

V

K

Q

G

E

G

L

E

G

M

L

P

S

D

A

F
x
P

V

L

H

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A

active site: K77 (= K44), S105 (= S72), D237 (= D207), S305 (= S269)
binding protoporphyrin ix containing fe: A182 (≠ Q151), P185 (= P154), L186 (≠ E155), Y189 (= Y158), R222 (= R191), T269 (≠ G233)
binding pyridoxal-5'-phosphate: K77 (= K44), N107 (= N74), G212 (= G181), T213 (= T182), G214 (= G183), T216 (= T185), G261 (= G225), S305 (= S269), P331 (= P295), D332 (= D296)
binding s-adenosylmethionine: P376 (≠ F340), G396 (= G360), F397 (≠ V361), D398 (≠ S362), Q399 (= Q363), T476 (= T445), I478 (≠ Q447), D479 (= D448)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 8, 9, 10, 11, 12, 20, 21, 22, 23

P35520 Cystathionine beta-synthase; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Homo sapiens (Human) (see 40 papers)
40% identity, 88% coverage: 2:402/455 of query aligns to 75:479/551 of P35520

query
sites
P35520

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P78 (≠ D5) to R: in CBSD; severe form; associated in cis with N-102; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204608
G85 (= G12) to R: in CBSD; loss of cystathionine beta-synthase activity; dbSNP:rs863223435
T87 (= T14) to N: in CBSD; decreased cystathionine beta-synthase activity; increased aggregation
L101 (= L26) to P: in CBSD; common mutation in Irish population; loss of activity; dbSNP:rs786204757
K102 (≠ A27) to N: in CBSD; associated in cis with R-78; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204609; to Q: in dbSNP:rs34040148
C109 (≠ V34) to R: in CBSD; loss of activity; dbSNP:rs778220779
A114 (≠ P39) to V: in CBSD; mild form; when linked with W-58 severe form; decreased cystathionine beta-synthase activity; decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs121964964
K119 (= K44) modified: N6-(pyridoxal phosphate)lysine
R125 (= R50) to Q: in CBSD; severe form; when linked with D-131 moderate form; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs781444670; to W: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs886057100
M126 (= M51) to V: in CBSD; loss of activity
E131 (= E56) to D: in CBSD; linked with Q-125; loss of activity; dbSNP:rs1555875351
G139 (= G64) to R: in CBSD; mild form; dbSNP:rs121964965
I143 (≠ V68) to M: in CBSD; 4% of activity; stable; dbSNP:rs370167302
E144 (= E69) to K: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964966
G148 (= G73) to R: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs755952006
N149 (= N74) binding pyridoxal 5'-phosphate
L154 (= L79) to Q: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
A155 (= A80) to T: in CBSD; complete loss of activity; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs1429138569; to V: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
C165 (= C90) to Y: in CBSD; severe form; protein expression is comparable to wild-type; loss of cystathionine beta-synthase activity; no effect on homotetramer formation; dbSNP:rs1347651454
M173 (≠ V98) to V: in CBSD; presents 40% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; natural variant: Missing (in CBSD; loss of activity)
E176 (≠ D101) to K: in CBSD; severe form; loss of cystathionine beta-synthase activity; inhibited by AdoMet; severely decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs762065361
V180 (= V105) to A: in CBSD; decreased cystathionine beta-synthase activity; decreases homotetramer formation; dbSNP:rs1555875010
T191 (≠ C116) to M: in CBSD; moderate and severe forms; loss of cystathionine beta-synthase activity; absent capacity to form multimeric quaternary structure; dbSNP:rs121964973
K211 (≠ A136) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
A226 (≠ Q151) to T: in CBSD; presents 20% of the wild-type activity; dramatically reduced capacity to form multimeric quaternary structure; dbSNP:rs763835246
N228 (= N153) to K: in CBSD; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs1464223176; to S: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1555874803
A231 (≠ S156) to P: in CBSD; has significantly decreased levels of enzyme activity
D234 (≠ H159) to N: in CBSD; decreased cystathionine beta-synthase activity; changed localization; decreased interaction with pyridoxal 5'-phosphate; no effect on homotetramer formation; dbSNP:rs773734233; natural variant: Missing (in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity)
GTGGT 256:260 (= GTGGT 181:185) binding pyridoxal 5'-phosphate
T257 (= T182) to M: in CBSD; moderate to severe form; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs758236584
T262 (≠ S187) to M: in CBSD; moderate form; dbSNP:rs149119723; to R: in CBSD; severe form; loss of cystathionine beta-synthase activity; loss of homotetramer formation
R266 (= R191) to K: in CBSD; mild form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; no effect on heme-binding; decreased stability; dbSNP:rs121964969
K269 (= K194) natural variant: Missing (in CBSD; loss of expression)
C272 (≠ S197) mutation to A: Reduced heme content and cystathionine beta-synthase activity.
C275 (≠ G201) to Y: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; mutation to S: Reduced heme content and cystathionine beta-synthase activity.
I278 (= I204) to S: in CBSD; loss of activity; to T: in CBSD; mild to severe form; common mutation; decreased expression; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs5742905
D281 (= D207) to N: in CBSD; loss of activity
A288 (vs. gap) to T: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs141502207
E302 (≠ L222) to K: in CBSD; no effect on cystathionine beta-synthase activity; inhibited by AdoHcy and impaired activation by AdoMet; no effect on homotetramer formation; dbSNP:rs779270933
G305 (= G225) to R: in CBSD; loss of cystathionine beta-synthase activity; no effect on homotetramer formation
G307 (= G227) to S: in CBSD; moderate to severe form; linked with D-534; common mutation; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; no effect on homotetramer formation; dbSNP:rs121964962
V320 (≠ C240) to A: in CBSD; has 36% of wild-type enzyme activity; dbSNP:rs781567152
D321 (= D241) to V: in CBSD; loss of activity
R336 (= R256) to C: in CBSD; protein expression is comparable to wild-type; loss of activity; absent capacity to form multimeric quaternary structure; dbSNP:rs398123151; to H: in CBSD; mild form; no effect on expression; exhibits an activity lower than 4% of the wild-type enzyme; altered stimulation by AdoMet; absent capacity to form multimeric quaternary structure; dbSNP:rs760417941
L338 (= L258) to P: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
G347 (= G267) to S: in CBSD; protein expression is comparable to wild-type; loss of activity; dbSNP:rs771298943
S349 (= S269) binding pyridoxal 5'-phosphate; to N: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
T353 (≠ A273) to M: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs121964972
R369 (≠ V289) to C: in CBSD; when linked with C-491 severe form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs117687681
D376 (= D296) to N: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1170128038
R379 (= R299) to Q: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs763036586
K384 (= K304) to E: in CBSD; severe form; dbSNP:rs121964967
P422 (≠ F340) to L: in CBSD; changed cystathionine beta-synthase activity; impaired stimulation by AdoMet; does not affect homotetramer formation; dbSNP:rs28934892
P427 (= P345) to L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs863223434
I435 (≠ V353) to T: in CBSD; no effect on cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; does not affect homotetramer formation
R439 (= R357) to Q: in CBSD; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs756467921
D444 (≠ S362) to N: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; increased homotetramer formation; dbSNP:rs28934891
V449 (≠ M367) to G: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
L456 (≠ V379) to P: in CBSD; severe; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
S466 (≠ D389) to L: in CBSD; increased cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964971

Sites not aligning to the query:

18 R → C: results in 1/3 to 2/3 the enzyme activity of the wild-type; dbSNP:rs201827340
49 P → L: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs148865119
52 binding axial binding residue
58 R → W: in CBSD; linked with V-114; 18% of activity; dbSNP:rs555959266
65 binding axial binding residue; H → R: in CBSD; decreased cystathionine beta-synthase activity; inhibited by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs1191141364
69 A → P: in dbSNP:rs17849313
500 S → L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs755106884
526 Q → K: in CBSD; has significantly decreased levels of enzyme activity
539 L → S: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs121964968
540 L → Q: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
548 R → Q: presents 60% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; dbSNP:rs150828989

1jbqA Structure of human cystathionine beta-synthase: a unique pyridoxal 5'- phosphate dependent hemeprotein (see paper)
44% identity, 69% coverage: 2:317/455 of query aligns to 33:348/348 of 1jbqA

query
sites
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E

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

I

P

P

G

T

T

V

Y

L

D

D

T

R

G

T

I

V

C

V

D

D

D

K

I

W

V

F

A

K

V

S

S

N

D

D

K

E

E

E

S

A

F

F

L

T

M

F

T

A

R

R

R

M

L

L

A

I

R

A

E

Q

E

E

A

G

L

L

V

C

G

G

S
|
S

C

A

G

G

L

S

A

T

V

V

E

A

A

V

A

A

L

V

R

K

V

A

A

A

E

Q

D

E

A

L

G

Q

P

E

D

G

D

Q

V

R

I

C

V

V

L

I

L

L

P
|
P

D
|
D

G

S

G

V

R

R

G

N

Y

Y

L

M

G

T

K

K

I

F

F

L

N

S

D

D

E

R

W

W

M

M

A

L

D

Q

Y

K

G

G

F

F

L

L

active site: K77 (= K44), S105 (= S72), D232 (= D207), S236 (= S211), L238 (vs. gap), S300 (= S269), P326 (= P295)
binding protoporphyrin ix containing fe: A177 (≠ Q151), P180 (= P154), L181 (≠ E155), Y184 (= Y158), R217 (= R191)
binding pyridoxal-5'-phosphate: K77 (= K44), N107 (= N74), V206 (≠ I180), G207 (= G181), T208 (= T182), G209 (= G183), G210 (= G184), T211 (= T185), G256 (= G225), S300 (= S269), P326 (= P295), D327 (= D296)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 9, 10, 11, 12, 16, 20, 21, 22, 23

6c4pA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the pmp complex (see paper)
46% identity, 67% coverage: 6:311/455 of query aligns to 9:330/344 of 6c4pA

query
sites
6c4pA

S

N

L

V

I

I

D

D

L

L

V

V

G

G

D

N

T

T

P

P

L

L

V

I

R

A

L

L

R

K

K

K

V

L

T

P

E

K

-

A

-

L

G

G

L

I

A

K

P

P

T

Q

I

I

L

Y

A

A

K

K

V

L

E

E

Y

L

F

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

I

A

A

L

K

R

S

M

M

V

V

E

E

A

E

A

A

E

E

K

A

S

S

G

G

E

R

L

I

R

H

-

P

A

S

G

R

G

S

T

T

I

L

V

I

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

L

V

I

A

G

Q

A

E

I

K

K

G

G

Y

Y

R

R

C

T

V

I

F

I

V

T

C

L

P

P

D

E

K

K

V

M

G

S

A

N

D

E

K

K

L

V

A

S

V

V

L

L

R

K

A

A

Y

L

G

G

A

A

E

E

V

I

V

R

C

T

P

P

T

T

T

A

V

A

A

A

P

W

E

D

H

S

P

P

D

E

S

S

Y

H

Y

I

S

G

V

V

S

A

D

K

R

K

L

L

A

E

E

K

E

E

I

I

P

P

G

G

A

A

W

V

K

I

P

L

N

D

Q

Q

Y

Y

E

N

N

N

Q

M

N

M

N

N

P

P

E

E

S

A

H

H

Y

Y

H

F

S

G

T

T

G

G

P

R

E

E

I

I

W

Q

D

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

R
x
N

V

L

T

R

H

A

F

V

V

V

A

A

G

G

I
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

S

G

G

T

I

G

S

R

K

Y

Y

L

L

K

K

E

E

V

Q

S

N

D

D

G

-

R

K

G

I

K

Q

V

I

I

V

G

G

A

A

D

D

P

P

E

F

G

G

S

S

V

I

Y

L

S

A

G

Q

G

P

S

E

G

N

-

L

-

N

-

K

-

T

-

D

-

I

R

T

P

D

Y

Y

L

K

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

V

P

P

G

Q

T

V

Y

L

D

D

T

R

G

K

I

L

C

I

D

D

D

V

I

W

V

Y

A

K

V

T

S

D

D

D

K

K

E

P

S

S

F

F

L

K

M

Y

T

A

R

R

R

Q

L

L

A

I

R

S

E

N

E

E

A

G

L

V

L

L

V

V

G

G

S
|
S

C

S

G

G

L

S

A

A

V

F

E

T

A

A

A

V

L

V

R

K

V

Y

A

C

E

E

D

D

-

H

-

P

-

E

A

L

G

T

P

E

D

D

V

V

I

I

V

V

V

A

L

I

L

F

P
|
P

D
|
D

G

S

G

I

R

R

G

S

Y

Y

L

L

G

T

K

K

I

F

F

V

N

D

D

D

E

E

W

W

M

L

binding calcium ion: N179 (vs. gap), D182 (≠ G171), N183 (≠ R172)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: K49 (= K44), N80 (= N74), A191 (≠ I180), G192 (= G181), T193 (= T182), G194 (= G183), T196 (= T185), G241 (= G225), S285 (= S269), P314 (= P295), D315 (= D296)

6c2zA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-aminoacrylate intermediate (see paper)
46% identity, 67% coverage: 6:311/455 of query aligns to 10:331/345 of 6c2zA

query
sites
6c2zA

S

N

L

V

I

I

D

D

L

L

V

V

G

G

D

N

T

T

P

P

L

L

V

I

R

A

L

L

R

K

K

K

V

L

T

P

E

K

-

A

-

L

G

G

L

I

A

K

P

P

T

Q

I

I

L

Y

A

A

K

K

V

L

E

E

Y

L

F

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

I

A

A

L

K

R

S

M

M

V

V

E

E

A

E

A

A

E

E

K

A

S

S

G

G

E

R

L

I

R

H

-

P

A

S

G

R

G

S

T

T

I

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

L

V

I

A

G

Q

A

E

I

K

K

G

G

Y

Y

R

R

C

T

V

I

F

I

V

T

C

L

P

P

D

E

K

K

V

M

G

S

A

N

D

E

K

K

L

V

A

S

V

V

L

L

R

K

A

A

Y

L

G

G

A

A

E

E

V

I

V

R

C

T

P

P

T

T

T

A

V

A

A

A

P

W

E

D

H

S

P

P

D

E

S

S

Y

H

Y

I

S

G

V

V

S

A

D

K

R

K

L

L

A

E

E

K

E

E

I

I

P

P

G

G

A

A

W

V

K

I

P

L

N

D

Q
|
Q

Y

Y

E

N

N

N

Q

M

N

M

N

N

P

P

E

E

S

A

H

H

Y

Y

H

F

S

G

T

T

G

G

P

R

E

E

I

I

W

Q

D

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

R
x
N

V

L

T

R

H

A

F

V

V

V

A

A

G

G

I
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

S

G

G

T

I

G

S

R

K

Y

Y

L

L

K

K

E

E

V

Q

S

N

D

D

G

-

R

K

G

I

K

Q

V

I

I

V

G

G

A

A

D

D

P

P

E

F

G

G

S

S

V

I

Y

L

S

A

G

Q

G

P

S

E

G

N

-

L

-

N

-

K

-

T

-

D

-

I

R

T

P

D

Y

Y

L

K

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

V

P

P

G

Q

T

V

Y

L

D

D

T

R

G

K

I

L

C

I

D

D

D

V

I

W

V

Y

A

K

V

T

S

D

D

D

K

K

E

P

S

S

F

F

L

K

M

Y

T

A

R

R

R

Q

L

L

A

I

R

S

E

N

E

E

A

G

L

V

L

L

V

V

G

G

S
|
S

C

S

G

G

L

S

A

A

V

F

E

T

A

A

A

V

L

V

R

K

V

Y

A

C

E

E

D

D

-

H

-

P

-

E

A

L

G

T

P

E

D

D

V

V

I

I

V

V

V

A

L

I

L

F

P
|
P

D
|
D

G

S

G

I

R

R

G

S

Y

Y

L

L

G

T

K

K

I

F

F

V

N

D

D

D

E

E

W

W

M

L

binding calcium ion: N180 (vs. gap), D183 (≠ G171), N184 (≠ R172)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K50 (= K44), T78 (= T71), S79 (= S72), N81 (= N74), T82 (= T75), Q154 (= Q147), A192 (≠ I180), G193 (= G181), T194 (= T182), G195 (= G183), T197 (= T185), G242 (= G225), S286 (= S269), P315 (= P295), D316 (= D296)

6c2qA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the plp-l-serine intermediate (see paper)
46% identity, 67% coverage: 6:311/455 of query aligns to 10:331/345 of 6c2qA

query
sites
6c2qA

S

N

L

V

I

I

D

D

L

L

V

V

G

G

D

N

T

T

P

P

L

L

V

I

R

A

L

L

R

K

K

K

V

L

T

P

E

K

-

A

-

L

G

G

L

I

A

K

P

P

T

Q

I

I

L

Y

A

A

K

K

V

L

E

E

Y

L

F

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

I

A

A

L

K

R

S

M

M

V

V

E

E

A

E

A

A

E

E

K

A

S

S

G

G

E

R

L

I

R

H

-

P

A

S

G

R

G

S

T

T

I

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

L

V

I

A

G

Q

A

E

I

K

K

G

G

Y

Y

R

R

C

T

V

I

F

I

V

T

C

L

P

P

D

E

K

K

V

M

G

S

A

N

D

E

K

K

L

V

A

S

V

V

L

L

R

K

A

A

Y

L

G

G

A

A

E

E

V

I

V

R

C

T

P

P

T

T

T

A

V

A

A

A

P

W

E

D

H

S

P

P

D

E

S

S

Y

H

Y

I

S

G

V

V

S

A

D

K

R

K

L

L

A

E

E

K

E

E

I

I

P

P

G

G

A

A

W

V

K

I

P

L

N

D

Q
|
Q

Y

Y

E

N

N

N

Q

M

N

M

N

N

P

P

E

E

S

A

H

H

Y

Y

H

F

S

G

T

T

G

G

P

R

E

E

I

I

W

Q

D

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

R
x
N

V

L

T

R

H

A

F

V

V

V

A

A

G

G

I
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

S

G

G

T

I

G

S

R

K

Y

Y

L

L

K

K

E

E

V

Q

S

N

D

D

G

-

R

K

G

I

K

Q

V

I

I

V

G

G

A

A

D

D

P

P

E

F

G

G

S

S

V

I

Y

L

S

A

G

Q

G

P

S

E

G

N

-

L

-

N

-

K

-

T

-

D

-

I

R

T

P

D

Y

Y

L

K

V

V

E

E

G
|
G

V

I

G

G

E
x
Y

D

D

I

F

W

V

P

P

G

Q

T

V

Y

L

D

D

T

R

G

K

I

L

C

I

D

D

D

V

I

W

V

Y

A

K

V

T

S

D

D

D

K

K

E

P

S

S

F

F

L

K

M

Y

T

A

R

R

R

Q

L

L

A

I

R

S

E

N

E

E

A

G

L

V

L

L

V

V

G

G

S
|
S

C

S

G

G

L

S

A

A

V

F

E

T

A

A

A

V

L

V

R

K

V

Y

A

C

E

E

D

D

-

H

-

P

-

E

A

L

G

T

P

E

D

D

V

V

I

I

V

V

V

A

L

I

L

F

P
|
P

D
|
D

G

S

G

I

R

R

G

S

Y

Y

L

L

G

T

K

K

I

F

F

V

N

D

D

D

E

E

W

W

M

L

binding calcium ion: N180 (vs. gap), D183 (≠ G171), N184 (≠ R172)
binding L-Serine, N-[[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinyl]methylene]: K50 (= K44), T78 (= T71), S79 (= S72), N81 (= N74), T82 (= T75), Q154 (= Q147), A192 (≠ I180), G193 (= G181), T194 (= T182), G195 (= G183), T197 (= T185), G242 (= G225), Y245 (≠ E228), S286 (= S269), P315 (= P295), D316 (= D296)

6c2hA Crystal structures of cystathionine beta-synthase from saccharomyces cerevisiae: the structure of the catalytic core (see paper)
46% identity, 67% coverage: 6:311/455 of query aligns to 10:331/345 of 6c2hA

query
sites
6c2hA

S

N

L

V

I

I

D

D

L

L

V

V

G

G

D

N

T

T

P

P

L

L

V

I

R

A

L

L

R

K

K

K

V

L

T

P

E

K

-

A

-

L

G

G

L

I

A

K

P

P

T

Q

I

I

L

Y

A

A

K

K

V

L

E

E

Y

L

F

Y

N

N

P

P

G

G

S

S

V

I

K
|
K

D

D

R

R

I

I

A

A

L

K

R

S

M

M

V

V

E

E

A

E

A

A

E

E

K

A

S

S

G

G

E

R

L

I

R

H

-

P

A

S

G

R

G

S

T

T

I

L

V

I

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

L

V

I

A

G

Q

A

E

I

K

K

G

G

Y

Y

R

R

C

T

V

I

F

I

V

T

C

L

P

P

D

E

K

K

V

M

G

S

A

N

D

E

K

K

L

V

A

S

V

V

L

L

R

K

A

A

Y

L

G

G

A

A

E

E

V

I

V

R

C

T

P

P

T

T

T

A

V

A

A

A

P

W

E

D

H

S

P

P

D

E

S

S

Y

H

Y

I

S

G

V

V

S

A

D

K

R

K

L

L

A

E

E

K

E

E

I

I

P

P

G

G

A

A

W

V

K

I

P

L

N

D

Q

Q

Y

Y

E

N

N

N

Q

M

N

M

N

N

P

P

E

E

S

A

H

H

Y

Y

H

F

S

G

T

T

G

G

P

R

E

E

I

I

W

Q

D

R

Q

Q

T

L

E

E

-

D

-

L

-
x
N

-

L

-

F

G
x
D

R
x
N

V

L

T

R

H

A

F

V

V

V

A

A

G

G

I
x
A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

S

G

G

T

I

G

S

R

K

Y

Y

L

L

K

K

E

E

V

Q

S

N

D

D

G

-

R

K

G

I

K

Q

V

I

I

V

G

G

A

A

D

D

P

P

E

F

G

G

S

S

V

I

Y

L

S

A

G

Q

G

P

S

E

G

N

-

L

-

N

-

K

-

T

-

D

-

I

R

T

P

D

Y

Y

L

K

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

V

P

P

G

Q

T

V

Y

L

D

D

T

R

G

K

I

L

C

I

D

D

D

V

I

W

V

Y

A

K

V

T

S

D

D

D

K

K

E

P

S

S

F

F

L

K

M

Y

T

A

R

R

R

Q

L

L

A

I

R

S

E

N

E

E

A

G

L

V

L

L

V

V

G

G

S
|
S

C

S

G

G

L

S

A

A

V

F

E

T

A

A

A

V

L

V

R

K

V

Y

A

C

E

E

D

D

-

H

-

P

-

E

A

L

G

T

P

E

D

D

V

V

I

I

V

V

V

A

L

I

L

F

P
|
P

D
|
D

G

S

G

I

R

R

G

S

Y

Y

L

L

G

T

K

K

I

F

F

V

N

D

D

D

E

E

W

W

M

L

binding calcium ion: N180 (vs. gap), D183 (≠ G171), N184 (≠ R172)
binding pyridoxal-5'-phosphate: K50 (= K44), N81 (= N74), A192 (≠ I180), G193 (= G181), T194 (= T182), G195 (= G183), T197 (= T185), G242 (= G225), S286 (= S269), P315 (= P295), D316 (= D296)

5ohxA Structure of active cystathionine b-synthase from apis mellifera (see paper)
37% identity, 100% coverage: 2:454/455 of query aligns to 30:486/488 of 5ohxA

query
sites
5ohxA

R

K

V

I

H

M

D

P

S

D

L

I

I

L

D

T

L

A

V

I

G

G

D

Q

T

T

P

P

L

L

V

I

R

K

L

L

R

N

K

N

V

I

-

P

-

K

T

S

E

Y

G

G

L

I

A

K

P

C

T

E

I

I

L

Y

A

A

K

K

V

C

E

E

Y

F

F

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

A

L

Y

R

R

M

M

V

I

E

Q

A

D

A

A

E

E

K

D

S

K

G

G

E

L

L

L

R

K

A

P

G

G

G

C

T

T

I

I

V

I

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

M

V

A

A

A

Q

A

E

V

K

R

G

G

Y

Y

R

K

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

D

D

E

K

K

L

I

A

S

V

T

L

L

R

Y

A

A

Y

L

G

G

A

A

E

K

V

I

V

R

C

T

P

P

T

T

T

E

V

A

A

S

P

W

E

H

H

S

P

P

D

E

S

A

Y

H

Y

I

S

S

V

V

S

A

D

Q

R

K

L

L

A

Q

E

K

E

E

I

I

P

P

G

N

A

S

W

I

K

I

P

L

N

D

Q

Q

Y

Y

E

T

N

N

Q
x
P

N

G

N

N

P
|
P

E

L

S

A

H

H

Y
|
Y

H

D

S

Q

T

T

G

A

P

I

E

E

I

I

W

W

D

K

Q

Q

T

C

E

E

G

G

R

K

V

I

T

D

H

Y

F

L

V

V

A

A

G
|
G

I

A

G
|
G

T
|
T

G
|
G

T
|
T

I

I

S

S

G

G

T

I

G

G

R
|
R

Y

K

L

L

K

K

E

E

V

L

S

S

D

P

G

N

R

I

G

-

K

K

V

I

I

I

G

A

A

V

D

D

P

P

E

K

G

G

S

S

V

I

Y

L

S

D

G

P

G

S

S

S

G

D

R

E

P

V

-

G

-

F

Y

Y

L

E

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

I

P

P

G

T

T

V

Y

L

D

D

T

R

G

N

I

V

C

I

D

D

D

K

I

W

V

I

A

K

V

T

S

E

D

D

K

N

E

E

S

S

F

L

L

N

M

A

T

A

R

R

R

M

L

L

A

I

R

R

E

Q

E

E

A

G

L

L

V

C

G

G

S
|
S

C

S

G

G

L

A

A

A

V

L

E

I

A

A

L

L

R

K

V

I

A

A

E

K

D

D

A

I

G

P

P

E

D

E

D

K

V

R

I

M

V

V

V

I

L

I

L

L

P
|
P

D
|
D

G

G

G

I

R

R

G

N

Y

Y

L

L

G

T

K

K

I

F

F

V

N

S

D

E

E

Y

W

W

M

M

A

E

D

T

Y

R

G

G

F

F

L

L

S

Q

A

P

E

V

T

C

E

Q

E

N

A

E

T

M

A

N

G

K

Q

W

V

W

L

W

N

N

G

M

K

K

G

I

G

S

E

N

M

L

P

S

D

F

F

D

V

K

H

Q

T

S

H

L

P

L

H

K

E

E

S

N

I

T

G

V

T

T

A

C

V

Q

A

E

I

A

M

M

R

H

-

M

-

L

E

K

Y

N

G

A

V

D

S

S

Q

Q

L

L

P

L

V

V

M

I

K

S

E

D

P

-

V

-

M

-

A

N

A

I

E

H

V

I

V

K

G

G

S

V

I

I

A

S

E

L

R

N

D

K

V

L

L

T

D

S

A

Y

L

V

F

I

D

S

G

G

R

I

A

V

Q

K

L

C

D

T

D

D

S

F

V

V

E

D

T

K

H

A

M

M

G

V

K

K

P

Q

L

Y

A

V

T

K

V

V

G

K

T

H

G

S

T

A

P

T

V

L

G

G

D

Y

C

I

V

S

R

R

L

V

L

L

R

E

G

K

S

E

G

P

A

Y

L

V

V

I

L

L

R

D

D

D

G

E

R

H

P

D

-

D

-

A

-

F

V

I

G

G

I

I

L

V

T

N

R

Q

Q

F

D

H

L

I

L

L

A

Q

H

F

L

I

S

T

binding protoporphyrin ix containing fe: P181 (≠ Q151), P184 (= P154), Y188 (= Y158), R221 (= R191)
binding pyridoxal-5'-phosphate: K74 (= K44), N104 (= N74), G209 (= G179), G211 (= G181), T212 (= T182), G213 (= G183), G214 (= G184), T215 (= T185), G256 (= G225), S300 (= S269), P326 (= P295), D327 (= D296)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 4, 7, 8, 9, 10, 14, 16, 17, 18, 19

Q2V0C9 Cystathionine beta-synthase; EC 4.2.1.22 from Apis mellifera (Honeybee) (see paper)
36% identity, 100% coverage: 2:454/455 of query aligns to 34:493/504 of Q2V0C9

query
sites
Q2V0C9

R

K

V

I

H

M

D

P

S

D

L

I

I

L

D

T

L

A

V

I

G

G

D

Q

T

T

P

P

L

L

V

I

R

K

L

L

R

N

K

N

V

I

-

P

-

K

T

S

E

Y

G

G

L

I

A

K

P

C

T

E

I

I

L

Y

A

A

K

K

V

C

E

E

Y

F

F

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

A

L

Y

R

R

M

M

V

I

E

Q

A

D

A

A

E

E

K

D

S

K

G

G

E

L

L

L

R

K

A

P

G

G

G

C

T

T

I

I

V

I

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

M

V

A

A

A

Q

A

E

V

K

R

G

G

Y

Y

R

K

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

D

D

E

K

K

L

I

A

S

V

T

L

L

R

Y

A

A

Y

L

G

G

A

A

E

K

V

I

V

R

C

T

P

P

T

T

T

E

V

A

A

S

P

W

E

H

H

S

P

P

D

E

S

A

Y

H

Y

I

S

S

V

V

S

A

D

Q

R

K

L

L

A

Q

E

K

E

E

I

I

P

P

G

N

A

S

W

I

K

I

P

L

N

D

Q

Q

Y

Y

E

T

N

N

Q

P

N

G

N

N

P

P

E

L

S

A

H

H

Y

Y

H

D

S

Q

T

T

G

A

P

I

E

E

I

I

W

W

D

K

Q

Q

T

C

E

E

G

G

R

K

V

I

T

D

H

Y

F

L

V

V

A

A

G

G

I

A

G
|
G

T
|
T

G
|
G

T
|
T

I

I

S

S

G

G

T

I

G

G

R

R

Y

K

L

L

K

K

E

E

V

L

S

S

D

P

G

N

R

I

G

-

K

K

V

I

I

I

G

A

A

V

D

D

P

P

E

K

G

G

S

S

V

I

Y

L

S

D

G

P

G

S

S

S

G

D

R

S

P

Q

-

N

-

E

-

V

-

G

-

F

Y

Y

L

E

V

V

E

E

G

G

V

I

G

G

E

Y

D

D

I

F

W

I

P

P

G

T

T

V

Y

L

D

D

T

R

G

N

I

V

C

I

D

D

D

K

I

W

V

I

A

K

V

T

S

E

D

D

K

N

E

E

S

S

F

L

L

N

M

A

T

A

R

R

R

M

L

L

A

I

R

R

E

Q

E

E

A

G

L

L

V

C

G

G

S
|
S

C

S

G

G

L

A

A

A

V

L

E

I

A

A

L

L

R

K

V

I

A

A

E

K

D

D

A

I

G

P

P

E

D

E

D

K

V

R

I

M

V

V

V

I

L

I

L

L

P

P

D

D

G

G

G

I

R

R

G

N

Y

Y

L

L

G

T

K

K

I

F

F

V

N

S

D

E

E

Y

W

W

M

M

A

E

D

T

Y

R

G

G

F

F

L

L

S

Q

A

P

E

V

T

C

E

Q

E

N

A

E

T

M

A

N

G

K

Q

W

V

W

L

W

N

N

G

M

K

K

G

I

G

S

E

N

M

L

P

S

D

F

F

D

V

K

H

Q

T

S

H

L

P

L

H

K

E

E

S

N

I

T

G

V

T

T

A

C

V

Q

A

E

I

A

M

M

R

H

-

M

-

L

E

K

Y

N

G

A

V

D

S

S

Q

Q

L

L

P

L

V

V

M

I

K

S

E

D

P

-

V

-

M

-

A

N

A

I

E

H

V

I

V

K

G

G

S

V

I

I

A

S

E

L

R

N

D

K

V

L

L

T

D

S

A

Y

L

V

F

I

D

S

G

G

R

I

A

V

Q

K

L

C

D

T

D

D

S

F

V

V

E

D

T

K

H

A

M

M

G

V

K

K

P

Q

L

Y

A

V

T

K

V

V

G

K

T

H

G

S

T

A

P

T

V

L

G

G

D

Y

C

I

V

S

R

R

L

V

L

L

R

E

G

K

S

E

G

P

A

Y

L

V

V

I

L

L

R

D

D

D

G

E

R

H

P

D

-

D

-

A

-

F

V

I

G

G

I

I

L

V

T

N

R

Q

Q

F

D

H

L

I

L

L

A

Q

H

F

L

I

S

T

K78 (= K44) modified: N6-(pyridoxal phosphate)lysine
N108 (= N74) binding pyridoxal 5'-phosphate
GTGGT 215:219 (= GTGGT 181:185) binding pyridoxal 5'-phosphate
S307 (= S269) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

12 binding axial binding residue
23 binding axial binding residue

6xwlC Cystathionine beta-synthase from toxoplasma gondii (see paper)
37% identity, 99% coverage: 3:454/455 of query aligns to 8:474/477 of 6xwlC

query
sites
6xwlC

V

I

H

L

D

D

S

S

L

V

I

L

D

D

L

A

V

I

G

G

D

N

T

T

P

P

L

M

V

V

R

R

L

M

R

K

K

R

V

L

T

A

E

K

-

V

-

Y

G

G

L

L

A

E

P

C

T

D

I

L

L

L

A

A

K

K

V

C

E

E

Y

F

F

M

N

S

P

A

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

L

K

R

A

M

M

V

V

E

E

A

K

A

A

E

E

K

R

S

E

G

G

E

R

L

L

R

K

A

A

G

G

G

D

T

T

I

L

V

I

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

L

V

A

A

A

Q

A

E

V

K

R

G

G

Y

Y

R

R

C

M

V

I

F

V

V

T

C

M

P

P

D

A

K

K

V

M

G

S

A

A

D

E

K

K

L

S

A

N

V

I

L

M

R

K

A

C

Y

L

G

G

A

A

E

E

V

I

V

V

V

R

C

T

P

P

T

T

T

E

V

A

A

A

P

W

E

N

H

D

P

E

D

N

S

S

Y

H

Y

M

S

G

V

V

S

A

D

A

R

K

L

L

A

Q

E

R

E

E

I

L

P

E

G

N

A

A

W

H

K

I

P

L

N

D

Q

Q

Y

Y

E

N

N

N

Q

T

N

A

N

N

P

P

E

M

S

V

H

H

Y

Y

H

D

S

V

T

T

G

A

P

E

E

E

I

I

W

I

D

T

Q

Q

T

C

E

D

G

G

R

D

V

I

T

D

H

M

F

V

V

V

A

I

G

G

I

A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

T

I

G

G

R

R

Y

K

L

I

K

K

E

E

V

R

S

C

D

P

G

-

R

K

G

C

K

K

V

V

I

V

G

G

A

V

D

D

P

P

E

K

G

G

S

S

V

I

Y

L

S

A

G

V

G

P

S

D

G

S

-

L

-

N

-

D

-

E

-

K

-

R

-

L

R

Q

P

S

Y

Y

L

E

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

V

P

P

G

G

T

V

Y

L

D

D

T

R

G

K

I

V

C

V

D

D

D

E

I

W

V

V

A

K

V

V

S

G

D

D

K

A

E

E

S

S

F

F

L

T

M

T

T

A

R

R

R

A

L

I

A

I

R

R

E

N

E

E

A

G

L

L

L

F

V

V

G

G

S
|
S

C

S

G

G

L

A

A

N

V

V

E

W

A

G

A

A

L

L

R

Q

V

A

A

A

E

R

D

Q

A

L

G

K

P

K

D

G

D

Q

V

K

I

C

V

V

L

L

P
|
P

D
|
D

G

S

R

R

G

N

Y

Y

L

M

G

S

K

K

I

F

F

I

N

S

D

D

E

E

W

W

M

M

A

A

D

E

Y

H

G

G

F

F

L

A

S

P

-

E

-

D

-

G

-

A

-

K

-

V

A

K

E

E

T

R

E

E

E

K

A

Q

T

F

A

G

G

G

-

A

-

R

-

I

Q

R

V

D

L

L

N

L

G

S

K

E

G

T

G

G

E

A

M

T

P

S

D

D

F

V

V

P

H

F

T

V

H

T

P

A

H

R

E

L

S

S

I

V

G

E

T

D

A

V

V

I

A

K

I

M

M

M

R

H

E

E

Y

T

G

K

V

V

S

K

Q

E

L

V

P

I

V

V

M

T

K

E

E

D

E

S

P

E

P

G

V

-

M

-

A

K

A

T

E

K

V

L

V

V

G

G

S

V

I

L

A

S

E

E

R

D

D

H

V

I

L

A

D

H

A

S

L

L

F

Q

D

S

G

G

R

R

A

C

Q

A

L

M

D

Q

D

S

S

P

V

V

E

K

T

D

H

I

M

A

G

F

K

K

P

K

L

L

A

A

T

K

V

A

G

L

T

P

G

S

T

A

P

Y

V

L

G

R

D

D

C

V

V

A

R

K

L

A

L

L

R

D

G

F

S

S

G

P

A

Y

L

V

V

C

V

V

L

M

R

D

D

C

G

P

R

H

P

F

V

L

G

G

I

V

L

I

T

T

R

R

Q

I

D

D

L

L

A

H

H

W

L

L

S

A

binding pyridoxal-5'-phosphate: K51 (= K44), N81 (= N74), G188 (= G181), T189 (= T182), G190 (= G183), T192 (= T185), G238 (= G225), S282 (= S269), P308 (= P295), D309 (= D296)

6xylA Crystal structure of delta466-491 cystathionine beta-synthase from toxoplasma gondii with l-serine (see paper)
37% identity, 99% coverage: 3:454/455 of query aligns to 8:465/468 of 6xylA

query
sites
6xylA

V

I

H

L

D

D

S

S

L

V

I

L

D

D

L

A

V

I

G

G

D

N

T

T

P

P

L

M

V

V

R

R

L

M

R

K

K

R

V

L

T

A

E

K

-

V

-

Y

G

G

L

L

A

E

P

C

T

D

I

L

L

L

A

A

K

K

V

C

E

E

Y

F

F

M

N

S

P

A

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

L

K

R

A

M

M

V

V

E

E

A

K

A

A

E

E

K

R

S

E

G

G

E

R

L

L

R

K

A

A

G

G

G

D

T

T

I

L

V

I

E

E

P

P

T

T

S

S

G

G

N

N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

L

V

A

A

A

Q

A

E

V

K

R

G

G

Y

Y

R

R

C

M

V

I

F

V

V

T

C

M

P

P

D

A

K

K

V

M

G

S

A

A

D

E

K

K

L

S

A

N

V

I

L

M

R

K

A

C

Y

L

G

G

A

A

E

E

V

I

V

V

V

R

C

T

P

P

T

T

T

E

V

A

A

A

P

W

E

N

H

D

P

E

D

N

S

S

Y

H

Y

M

S

G

V

V

S

A

D

A

R

K

L

L

A

Q

E

R

E

E

I

L

P

E

G

N

A

A

W

H

K

I

P

L

N

D

Q
|
Q

Y

Y

E

N

N

N

Q

T

N

A

N

N

P

P

E

M

S

V

H

H

Y

Y

H

D

S

V

T

T

G

A

P

E

E

E

I

I

W

I

D

T

Q

Q

T

C

E

D

G

G

R

D

V

I

T

D

H

M

F

V

V

V

A

I

G

G

I

A

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

T

I

G

G

R

R

Y

K

L

I

K

K

E

E

V

R

S

C

D

P

G

-

R

K

G

C

K

K

V

V

I

V

G

G

A

V

D

D

P

P

E

K

G

G

S

S

V

I

Y

L

S

A

G

V

G

P

S

D

G

S

-

L

-

N

-

D

-

E

-

K

-

R

-

L

R

Q

P

S

Y

Y

L

E

V

V

E

E

G
|
G

V
x
I

G

G

E
x
Y

D

D

I

F

W

V

P

P

G

G

T

V

Y

L

D

D

T

R

G

K

I

V

C

V

D

D

D

E

I

W

V

V

A

K

V

V

S

G

D

D

K

A

E

E

S

S

F

F

L

T

M

T

T

A

R

R

R

A

L

I

A

I

R

R

E

N

E

E

A

G

L

L

L

F

V

V

G

G

S
|
S

C

S

G

G

L

A

A

N

V

V

E

W

A

G

A

A

L

L

R

Q

V

A

A

A

E

R

D

Q

A

L

G

K

P

K

D

G

D

Q

V

K

I

C

V

V

L

L

P
|
P

D
|
D

G

S

R

R

G

N

Y

Y

L

M

G

S

K

K

I

F

F

I

N

S

D

D

E

E

W

W

M

M

A

A

D

E

Y

H

G

G

F

F

L

A

S

P

A

E

E

D

T

G

E

A

E

K

A

V

T

K

A

E

G

R

Q

E

V

K

L

Q

N

F

G

G

K

-

G

G

G

A

E

R

M

I

P

R

D

D

F

L

V

L

H

S

T

E

H

T

P

G

H

A

E

T

S

S

I

F

G

V

T

T

A

A

-

R

-

L

-

S

-

V

-

E

-

D

-

V

V

I

A

K

I

M

M

M

R

H

E

E

Y

T

G

K

V

V

S

K

Q

E

L

V

P

I

V

V

M

T

K

E

E

D

E

-

P

-

V

-

M

-

A

-

E

S

V

L

V

V

G

G

S

V

I

L

A

S

E

E

R

D

D

H

V

I

L

A

D

H

A

S

L

L

F

Q

D

S

G

G

R

R

A

C

Q

A

L

M

D

Q

D

S

S

P

V

V

E

K

T

D

H

I

M

A

G

F

K

K

P

K

L

L

A

A

T

K

V

A

G

L

T

P

G

S

T

A

P

Y

V

L

G

R

D

D

C

V

V

A

R

K

L

A

L

L

R

D

G

F

S

S

G

P

A

Y

L

V

V

C

V

V

L

M

R

C

D

P

G

-

R

H

P

F

V

L

G

G

I

V

L

I

T

T

R

R

Q

I

D

D

L

L

A

H

H

W

L

L

S

A

binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K51 (= K44), T82 (= T75), Q154 (= Q147), G188 (= G181), T189 (= T182), G190 (= G183), T192 (= T185), G238 (= G225), I239 (≠ V226), Y241 (≠ E228), S282 (= S269), P308 (= P295), D309 (= D296)

3pc2A Full length structure of cystathionine beta-synthase from drosophila (see paper)
35% identity, 95% coverage: 2:435/455 of query aligns to 36:473/500 of 3pc2A

query
sites
3pc2A

R

Q

V

I

H

T

D

P

S

N

L

I

I

L

D

E

L

V

V

I

G

G

D

C

T

T

P

P

L

L

V

V

R

K

L

L

R

N

K

N

V

I

-

P

-

A

T

S

E

D

G

G

L

I

A

E

P

C

T

E

I

M

L

Y

A

A

K

K

V

C

E

E

Y

F

F

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

L

Y

R

R

M

M

V

V

E

Q

A

D

A

A

E

E

K

E

S

Q

G

G

E

L

L

L

R

K

A

P

G

G

G

Y

T

T

I

I

V

I

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

M

M

V

A

A

C

Q

A

E

V

K

K

G

G

Y

Y

R

K

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

N

D

E

K

K

L

V

A

S

V

A

L

L

R

R

A

T

Y

L

G

G

A

A

E

K

V

I

V

R

C

T

P

P

T

T

T

E

V

A

A

A

P

Y

E

D

H

S

P

P

D

E

S

G

Y

L

Y

I

S

Y

V

V

S

A

D

Q

R

Q

L

L

A

Q

E

R

E

E

I

T

P

P

G

N

A

S

W

I

K

V

P

L

N

D

Q

Q

Y

Y

E

R

N

N

Q
x
A

N

G

N

N

P
|
P

E
x
L

S

A

H

H

Y
|
Y

H

D

S

G

T

T

G

A

P

A

E

E

I

I

W

L

D

W

Q

Q

T

L

E

D

G

N

R

K

V

V

T

D

H

M

F

I

V

V

A

V

G

S

I
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

I

I

S

S

G

G

T

I

G

G

R
|
R

Y

K

L

I

K

K

E

E

V

Q

S

V

D

P

G

S

R

-

G

C

K

Q

V

I

I

V

G

G

A

V

D

D

P

P

E

Y

G

G

S

S

V

I

Y

L

S

A

G

-

S

-

G

-

R

R

P

P

-

A

-

E

-

L

-

N

-

K

-

T

-

D

-

V

-

Q

-

F

Y

Y

L

E

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

P

P

P

G

T

T

V

Y

F

D

D

T

D

G

T

I

V

C

V

D

D

D

V

I

W

V

T

A

K

V

I

S

G

D

D

K

S

E

D

S

C

F

F

L

P

M

M

T

S

R

R

L

L

A

N

R

A

E

E

A

G

L

L

V

C

G

G

S
|
S

C

S

G

G

L

G

A

A

V

M

E

H

A

A

L

L

R

E

V

H

A

A

E

R

D

K

A

L

G

K

P

K

D

G

D

Q

V

R

I

C

V

V

L

I

L

L

P
|
P

D
|
D

G

G

G

I

R

R

G

N

Y

Y

L

M

G

T

K

K

I

F

F

V

N

S

D

D

E

N

W

W

M

M

A

E

D

A

Y

R

G

N

F

F

L

K

S

E

A

P

E

V

T

N

E

E

E

H

A

G

T

H

A

W

G

W

Q

W

V

S

L

L

N

A

G

I

K

A

G

E

G

L

E

E

M

L

P

P

-

A

D

P

F

P

V

V

H

I

T

L

H

K

P

S

H

D

E

A

S

T

I

V

G

G

T

E

A

A

V

I

A

A

I

L

M

M

R

K

E

K

Y

H

G

R

V

V

S

D

Q

Q

L

L

P

P

V

V

M

V

K

D

E

Q

E

D

P

-

V

-

M

-

A

D

A

G

E

S

V

V

V

L

G

G

S

V

I

V

A

G

E

Q

R

E

D

T

V

L

L

I

D

T

A

Q

L

I

F

V

D

S

G

M

R

N

A

R

Q

Q

L

Q

D

S

D

D

S

P

V

A

E

I

T

K

H

A

M

L

G

N

K

K

P

R

L

V

A

I

T

R

V

L

G

N

T

E

G

S

T

E

P

I

V

L

G

G

D

K

C

L

V

A

R

R

L

V

L

L

R

E

G

V

S

D

G

P

A

S

L

V

V

L

V

I

L

L

active site: K80 (= K44), S310 (= S269)
binding protoporphyrin ix containing fe: A187 (≠ Q151), P190 (= P154), L191 (≠ E155), Y194 (= Y158), R227 (= R191)
binding pyridoxal-5'-phosphate: K80 (= K44), N110 (= N74), A216 (≠ I180), G217 (= G181), T218 (= T182), A219 (≠ G183), T221 (= T185), G266 (= G225), S310 (= S269), P336 (= P295), D337 (= D296)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 12, 13, 14, 15, 16, 24, 25, 26

3pc4A Full length structure of cystathionine beta-synthase from drosophila in complex with serine (see paper)
35% identity, 95% coverage: 2:435/455 of query aligns to 38:475/504 of 3pc4A

query
sites
3pc4A

R

Q

V

I

H

T

D

P

S

N

L

I

I

L

D

E

L

V

V

I

G

G

D

C

T

T

P

P

L

L

V

V

R

K

L

L

R

N

K

N

V

I

-

P

-

A

T

S

E

D

G

G

L

I

A

E

P

C

T

E

I

M

L

Y

A

A

K

K

V

C

E

E

Y

F

F

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

L

Y

R

R

M

M

V

V

E

Q

A

D

A

A

E

E

K

E

S

Q

G

G

E

L

L

L

R

K

A

P

G

G

G

Y

T

T

I

I

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

V

A

A

C

Q

A

E

V

K

K

G

G

Y

Y

R

K

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

N

D

E

K

K

L

V

A

S

V

A

L

L

R

R

A

T

Y

L

G

G

A

A

E

K

V

I

V

R

C

T

P

P

T

T

T

E

V

A

A

A

P

Y

E

D

H

S

P

P

D

E

S

G

Y

L

Y

I

S

Y

V

V

S

A

D

Q

R

Q

L

L

A

Q

E

R

E

E

I

T

P

P

G

N

A

S

W

I

K

V

P

L

N

D

Q
|
Q

Y

Y

E

R

N

N

Q
x
A

N

G

N

N

P
|
P

E
x
L

S

A

H

H

Y
|
Y

H

D

S

G

T

T

G

A

P

A

E

E

I

I

W

L

D

W

Q

Q

T

L

E

D

G

N

R

K

V

V

T

D

H

M

F

I

V

V

A

V

G

S

I
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

I

I

S

S

G

G

T

I

G

G

R
|
R

Y

K

L

I

K

K

E

E

V

Q

S

V

D

P

G

S

R

-

G

C

K

Q

V

I

I

V

G

G

A

V

D

D

P

P

E

Y

G

G

S

S

V

I

Y

L

S

A

G

-

S

-

G

-

R

R

P

P

-

A

-

E

-

L

-

N

-

K

-

T

-

D

-

V

-

Q

-

F

Y

Y

L

E

V

V

E

E

G
|
G

V
x
I

G

G

E
x
Y

D

D

I

F

W

P

P

P

G
x
T

T

V

Y

F

D

D

T

D

G

T

I

V

C

V

D

D

D

V

I

W

V

T

A

K

V

I

S

G

D

D

K

S

E

D

S

C

F

F

L

P

M

M

T

S

R

R

L

L

A

N

R

A

E

E

A

G

L

L

V

C

G

G

S
|
S

C

S

G

G

L

G

A

A

V

M

E

H

A

A

L

L

R

E

V

H

A

A

E

R

D

K

A

L

G

K

P

K

D

G

D

Q

V

R

I

C

V

V

L

I

L

L

P
|
P

D
|
D

G

G

G

I

R

R

G

N

Y

Y

L

M

G

T

K

K

I

F

F

V

N

S

D

D

E

N

W

W

M

M

A

E

D

A

Y

R

G

N

F

F

L

K

S

E

A

P

E

V

T

N

E

E

E

H

A

G

T

H

A

W

G

W

Q

W

V

S

L

L

N

A

G

I

K

A

G

E

G

L

E

E

M

L

P

P

-

A

D

P

F

P

V

V

H

I

T

L

H

K

P

S

H

D

E

A

S

T

I

V

G

G

T

E

A

A

V

I

A

A

I

L

M

M

R

K

E

K

Y

H

G

R

V

V

S

D

Q

Q

L

L

P

P

V

V

M

V

K

D

E

Q

E

D

P

-

V

-

M

-

A

D

A

G

E

S

V

V

V

L

G

G

S

V

I

V

A

G

E

Q

R

E

D

T

V

L

L

I

D

T

A

Q

L

I

F

V

D

S

G

M

R

N

A

R

Q

Q

L

Q

D

S

D

D

S

P

V

A

E

I

T

K

H

A

M

L

G

N

K

K

P

R

L

V

A

I

T

R

V

L

G

N

T

E

G

S

T

E

P

I

V

L

G

G

D

K

C

L

V

A

R

R

L

V

L

L

R

E

G

V

S

D

G

P

A

S

L

V

V

L

V

I

L

L

active site: K82 (= K44), S312 (= S269)
binding protoporphyrin ix containing fe: A189 (≠ Q151), P192 (= P154), L193 (≠ E155), Y196 (= Y158), R229 (= R191), T276 (≠ G233)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: K82 (= K44), T109 (= T71), S110 (= S72), N112 (= N74), T113 (= T75), Q185 (= Q147), A218 (≠ I180), G219 (= G181), T220 (= T182), A221 (≠ G183), T223 (= T185), G268 (= G225), I269 (≠ V226), Y271 (≠ E228), S312 (= S269), P338 (= P295), D339 (= D296)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc3A Full length structure of cystathionine beta-synthase from drosophila in complex with aminoacrylate (see paper)
35% identity, 95% coverage: 2:435/455 of query aligns to 38:475/504 of 3pc3A

query
sites
3pc3A

R

Q

V

I

H

T

D

P

S

N

L

I

I

L

D

E

L

V

V

I

G

G

D

C

T

T

P

P

L

L

V

V

R

K

L

L

R

N

K

N

V

I

-

P

-

A

T

S

E

D

G

G

L

I

A

E

P

C

T

E

I

M

L

Y

A

A

K

K

V

C

E

E

Y

F

F

L

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

L

Y

R

R

M

M

V

V

E

Q

A

D

A

A

E

E

K

E

S

Q

G

G

E

L

L

L

R

K

A

P

G

G

G

Y

T

T

I

I

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

M

M

V

A

A

C

Q

A

E

V

K

K

G

G

Y

Y

R

K

C

C

V

I

F

I

V

V

C

M

P

P

D

E

K

K

V

M

G

S

A

N

D

E

K

K

L

V

A

S

V

A

L

L

R

R

A

T

Y

L

G

G

A

A

E

K

V

I

V

R

C

T

P

P

T

T

T

E

V

A

A

A

P

Y

E

D

H

S

P

P

D

E

S

G

Y

L

Y

I

S

Y

V

V

S

A

D

Q

R

Q

L

L

A

Q

E

R

E

E

I

T

P

P

G

N

A

S

W

I

K

V

P

L

N

D

Q
|
Q

Y

Y

E

R

N

N

Q
x
A

N

G

N

N

P
|
P

E
x
L

S

A

H

H

Y
|
Y

H

D

S

G

T

T

G

A

P

A

E

E

I

I

W

L

D

W

Q

Q

T

L

E

D

G

N

R

K

V

V

T

D

H

M

F

I

V

V

A

V

G

S

I
x
A

G
|
G

T
|
T

G
x
A

G

G

T
|
T

I

I

S

S

G

G

T

I

G

G

R
|
R

Y

K

L

I

K

K

E

E

V

Q

S

V

D

P

G

S

R

-

G

C

K

Q

V

I

I

V

G

G

A

V

D

D

P

P

E

Y

G

G

S

S

V

I

Y

L

S

A

G

-

S

-

G

-

R

R

P

P

-

A

-

E

-

L

-

N

-

K

-

T

-

D

-

V

-

Q

-

F

Y

Y

L

E

V

V

E

E

G
|
G

V
x
I

G

G

E

Y

D

D

I

F

W

P

P

P

G

T

T

V

Y

F

D

D

T

D

G

T

I

V

C

V

D

D

D

V

I

W

V

T

A

K

V

I

S

G

D

D

K

S

E

D

S

C

F

F

L

P

M

M

T

S

R

R

L

L

A

N

R

A

E

E

A

G

L

L

V

C

G

G

S
|
S

C

S

G

G

L

G

A

A

V

M

E

H

A

A

L

L

R

E

V

H

A

A

E

R

D

K

A

L

G

K

P

K

D

G

D

Q

V

R

I

C

V

V

L

I

L

L

P
|
P

D
|
D

G

G

G

I

R

R

G

N

Y

Y

L

M

G

T

K

K

I

F

F

V

N

S

D

D

E

N

W

W

M

M

A

E

D

A

Y

R

G

N

F

F

L

K

S

E

A

P

E

V

T

N

E

E

E

H

A

G

T

H

A

W

G

W

Q

W

V

S

L

L

N

A

G

I

K

A

G

E

G

L

E

E

M

L

P

P

-

A

D

P

F

P

V

V

H

I

T

L

H

K

P

S

H

D

E

A

S

T

I

V

G

G

T

E

A

A

V

I

A

A

I

L

M

M

R

K

E

K

Y

H

G

R

V

V

S

D

Q

Q

L

L

P

P

V

V

M

V

K

D

E

Q

E

D

P

-

V

-

M

-

A

D

A

G

E

S

V

V

V

L

G

G

S

V

I

V

A

G

E

Q

R

E

D

T

V

L

L

I

D

T

A

Q

L

I

F

V

D

S

G

M

R

N

A

R

Q

Q

L

Q

D

S

D

D

S

P

V

A

E

I

T

K

H

A

M

L

G

N

K

K

P

R

L

V

A

I

T

R

V

L

G

N

T

E

G

S

T

E

P

I

V

L

G

G

D

K

C

L

V

A

R

R

L

V

L

L

R

E

G

V

S

D

G

P

A

S

L

V

V

L

V

I

L

L

active site: K82 (= K44), S312 (= S269)
binding protoporphyrin ix containing fe: A189 (≠ Q151), P192 (= P154), L193 (≠ E155), Y196 (= Y158), R229 (= R191)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K82 (= K44), T109 (= T71), S110 (= S72), N112 (= N74), T113 (= T75), Q185 (= Q147), A218 (≠ I180), G219 (= G181), T220 (= T182), A221 (≠ G183), T223 (= T185), G268 (= G225), I269 (≠ V226), S312 (= S269), P338 (= P295), D339 (= D296)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

6vjuB Crystal structure of cystathionine beta synthase from legionella pneumophila with llp, plp, and homocysteine
44% identity, 69% coverage: 3:317/455 of query aligns to 3:317/317 of 6vjuB

query
sites
6vjuB

V

I

H

Y

D

P

S

N

L

I

I

L

D

A

L

T

V

I

G

G

D

H

T

T

P

P

L

V

V

V

R

K

L

I

R

N

K

R

V

L

T

G

E

K

G

D

L

L

A

E

P

C

T

E

I

L

L

Y

A

A

K

K

V

C

E

E

Y

F

F

F

N

N

P

P

G

G

S

S

V

V

K
|
K

D

D

R

R

I

I

A

G

L

Y

R

E

M

M

V

V

E

V

A

K

A

A

E

E

K

K

S

E

G

G

E

R

L

I

R

K

A

P

G

G

G

D

T

T

I

L

V

I

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

I

A

A

M

L

V

A

A

G

Q

A

E

V

K

L

G

G

Y

Y

R

K

C

V

V

I

F

I

V

T

C

M

P

P

D

E

K

K

V

M

G

S

A

Q

D

E

K

K

L

Q

A

S

V

V

L

L

R

E

A

R

Y

L

G

G

A

A

E

I

V

I

V

Y

V

R

C

T

P

P

T

T

T

E

V

A

A

A

P

Y

E

N

H

D

P

P

D

D

S

S

Y

H

Y

I

S

S

V

L

S

A

D

K

R

K

L

L

A

Q

E

A

E

E

I

I

P

P

G

N

A

S

W

H

K

I

P

L

N

D

Q

Q

Y

Y

E

A

N

N

Q

P

N

N

P

P

E

N

S

A

H

H

Y

Y

H

F

S

G

T

T

G

A

P

Q

E

E

I

I

W

I

D

D

Q

D

T

F

E

G

G

K

R

D

V

L

T

H

H

M

F

V

V

V

A

A

G

G

I

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

S

T

G

G

T

I

G

A

R

K

Y

R

L

L

K

K

E

E

V

F

S

N

D

P

G

A

R

I

G

-

K

K

V

I

I

I

G

G

A

A

D

D

P

P

E

E

G

G

S

S

V

I

Y

L

S

G

G

G

S

T

G

E

-

I

R

K

P

S

Y

Y

L

H

V

V

E

E

G
|
G

V

I

G

G

E

Y

D

D

I

F

W

F

P

P

G

D

T

V

Y

L

D

D

T

N

G

T

I

L

C

I

D

D

D

A

I

Y

V

I

A

K

V

T

S

N

D

D

K

A

E

D

S

S

F

F

L

R

M

T

T

A

R

R

L

L

A

I

R

K

E

E

A

G

L

L

V

I

G

G

S
|
S

C

C

G

G

L

A

A

A

V

M

E

W

A

A

L

L

R

Q

V

A

A

A

E

K

D

S

A

L

G

S

P

K

D

G

D

Q

V

K

I

C

V

L

V

V

L

I

L

L

P
|
P

D
|
D

G

S

G

I

R

R

G

N

Y

Y

L

M

G

S

K

K

I

F

F

A

N

N

D

D

E

E

W

W

M

M

A

K

D

E

Y

M

G

G

F

F

L

L

binding pyridoxal-5'-phosphate: K44 (= K44), N74 (= N74), G181 (= G181), T182 (= T182), G183 (= G183), T185 (= T185), G225 (= G225), S269 (= S269), P295 (= P295), D296 (= D296)

Query Sequence

>WP_017598059.1 NCBI__GCF_000341125.1:WP_017598059.1
MRVHDSLIDLVGDTPLVRLRKVTEGLAPTILAKVEYFNPGGSVKDRIALRMVEAAEKSGE
LRAGGTIVEPTSGNTGIGLAMVAQEKGYRCVFVCPDKVGADKLAVLRAYGAEVVVCPTTV
APEHPDSYYSVSDRLAEEIPGAWKPNQYENQNNPESHYHSTGPEIWDQTEGRVTHFVAGI
GTGGTISGTGRYLKEVSDGRGKVIGADPEGSVYSGGSGRPYLVEGVGEDIWPGTYDTGIC
DDIVAVSDKESFLMTRRLAREEALLVGGSCGLAVEAALRVAEDAGPDDVIVVLLPDGGRG
YLGKIFNDEWMADYGFLSAETEEATAGQVLNGKGGEMPDFVHTHPHESIGTAVAIMREYG
VSQLPVMKEEPPVMAAEVVGSIAERDVLDALFDGRAQLDDSVETHMGKPLATVGTGTPVG
DCVRLLRGSGALVVLRDGRPVGILTRQDLLAHLSG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory