SitesBLAST
Comparing WP_017617176.1 NCBI__GCF_002263495.1:WP_017617176.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
70% identity, 100% coverage: 2:254/254 of query aligns to 6:258/258 of 4wecA
- active site: G21 (= G17), S143 (= S139), Q154 (= Q150), Y157 (= Y153), K161 (= K157)
- binding nicotinamide-adenine-dinucleotide: G17 (= G13), A19 (≠ G15), S20 (= S16), G21 (= G17), I22 (= I18), D41 (= D37), I42 (≠ L38), V61 (≠ A57), D62 (= D58), V63 (= V59), N89 (= N85), T141 (= T137), Y157 (= Y153), K161 (= K157), P187 (= P183), P189 (= P185), V190 (= V186)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
40% identity, 97% coverage: 1:246/254 of query aligns to 2:242/244 of 4nbuB
- active site: G18 (= G17), N111 (= N111), S139 (= S139), Q149 (= Q150), Y152 (= Y153), K156 (= K157)
- binding acetoacetyl-coenzyme a: D93 (= D94), K98 (vs. gap), S139 (= S139), N146 (≠ A147), V147 (≠ T148), Q149 (= Q150), Y152 (= Y153), F184 (≠ P185), M189 (≠ L190), K200 (≠ R205)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G13), N17 (≠ S16), G18 (= G17), I19 (= I18), D38 (= D37), F39 (≠ L38), V59 (≠ A57), D60 (= D58), V61 (= V59), N87 (= N85), A88 (= A86), G89 (= G87), I90 (= I88), T137 (= T137), S139 (= S139), Y152 (= Y153), K156 (= K157), P182 (= P183), F184 (≠ P185), T185 (≠ V186), T187 (= T188), M189 (≠ L190)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
41% identity, 98% coverage: 3:250/254 of query aligns to 10:267/267 of Q9LBG2
- 17:42 (vs. 10:35, 58% identical) binding NAD(+)
- E103 (≠ S89) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
1iy8A Crystal structure of levodione reductase (see paper)
41% identity, 98% coverage: 3:250/254 of query aligns to 1:258/258 of 1iy8A
- active site: G15 (= G17), S143 (= S139), Q153 (= Q150), Y156 (= Y153), K160 (= K157)
- binding nicotinamide-adenine-dinucleotide: G11 (= G13), S14 (= S16), G15 (= G17), L16 (≠ I18), D35 (= D37), V36 (≠ L38), A62 (= A57), D63 (= D58), V64 (= V59), N90 (= N85), G92 (= G87), I93 (= I88), T141 (= T137), S143 (= S139), Y156 (= Y153), K160 (= K157), P186 (= P183), G187 (= G184), T191 (= T188), P192 (= P189), M193 (≠ L190)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
40% identity, 99% coverage: 2:253/254 of query aligns to 1:248/251 of H9XP47
- N15 (≠ S16) binding NAD(+)
- M17 (≠ I18) binding NAD(+)
- D36 (= D37) binding NAD(+)
- D60 (= D58) binding NAD(+)
- V61 (= V59) binding NAD(+)
- N87 (= N85) binding NAD(+)
- S138 (= S139) binding (R)-acetoin; binding (S)-acetoin
- V139 (= V141) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ A142) binding (R)-acetoin
- Y151 (= Y153) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
- K155 (= K157) binding NAD(+)
- V184 (= V186) binding NAD(+)
- T186 (= T188) binding NAD(+)
- RDK 197:199 (≠ FAK 195:197) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
- Q247 (≠ T252) mutation to A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
6vspB Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
39% identity, 99% coverage: 3:253/254 of query aligns to 4:250/252 of 6vspB
6vspA Structure of serratia marcescens 2,3-butanediol dehydrogenase mutant q247a (see paper)
39% identity, 99% coverage: 3:253/254 of query aligns to 2:248/251 of 6vspA
- active site: G16 (= G17), S138 (= S139), Y151 (= Y153)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ S16), G16 (= G17), M17 (≠ I18), D36 (= D37), W37 (≠ L38), W37 (≠ L38), A38 (≠ D39), I59 (≠ A57), D60 (= D58), V61 (= V59), N87 (= N85), A88 (= A86), G89 (= G87), V90 (≠ I88), V110 (= V110), T136 (= T137), S138 (= S139), Y151 (= Y153), K155 (= K157), P181 (= P183), S182 (≠ G184), L183 (≠ P185), V184 (= V186), T186 (= T188), N187 (≠ P189), M188 (≠ L190), T189 (≠ L191)
6xewA Structure of serratia marcescens 2,3-butanediol dehydrogenase (see paper)
40% identity, 99% coverage: 3:253/254 of query aligns to 2:248/251 of 6xewA
- active site: G16 (= G17), S138 (= S139), Y151 (= Y153)
- binding r,3-hydroxybutan-2-one: S138 (= S139), S140 (≠ A142), Y151 (= Y153)
- binding s,3-hydroxybutan-2-one: S138 (= S139), Y151 (= Y153), S182 (≠ G184)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), N15 (≠ S16), G16 (= G17), M17 (≠ I18), D36 (= D37), W37 (≠ L38), W37 (≠ L38), A38 (≠ D39), I59 (≠ A57), D60 (= D58), V61 (= V59), N87 (= N85), A88 (= A86), G89 (= G87), V110 (= V110), T136 (= T137), S138 (= S139), Y151 (= Y153), K155 (= K157), S182 (≠ G184), L183 (≠ P185), V184 (= V186), T186 (= T188), N187 (≠ P189), M188 (≠ L190), T189 (≠ L191)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
39% identity, 95% coverage: 5:245/254 of query aligns to 4:244/248 of 6ixmC
- active site: G16 (= G17), S142 (= S139), Y155 (= Y153), K159 (= K157)
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), S15 (= S16), G16 (= G17), I17 (= I18), D36 (= D37), I37 (≠ L38), A61 (= A57), D62 (= D58), T63 (≠ V59), N89 (= N85), A90 (= A86), M140 (≠ T137), S142 (= S139), Y155 (= Y153), K159 (= K157), P185 (= P183), A186 (≠ G184), Y187 (≠ P185), I188 (≠ V186), L192 (= L190)
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme
37% identity, 96% coverage: 1:245/254 of query aligns to 1:245/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (= G13), S16 (= S16), G17 (= G17), I18 (= I18), D37 (= D37), I38 (≠ L38), A62 (= A57), D63 (= D58), S64 (≠ V59), N90 (= N85), M141 (≠ T137), Y156 (= Y153), K160 (= K157), P186 (= P183), G187 (= G184), Y188 (≠ P185), I189 (≠ V186), L193 (= L190)
5jc8D Crystal structure of a putative short-chain dehydrogenase/reductase from burkholderia xenovorans
38% identity, 96% coverage: 3:247/254 of query aligns to 3:258/262 of 5jc8D
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
38% identity, 95% coverage: 5:246/254 of query aligns to 7:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), S17 (≠ G15), R18 (≠ S16), I20 (= I18), T40 (≠ L38), N62 (≠ D58), V63 (= V59), N89 (= N85), A90 (= A86), I92 (= I88), V139 (≠ T137), S141 (= S139), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), I187 (≠ V186), T189 (= T188), M191 (≠ L190)
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
41% identity, 94% coverage: 6:245/254 of query aligns to 5:247/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G13), G16 (= G17), I17 (= I18), D36 (= D37), L37 (= L38), C61 (≠ A57), D62 (= D58), V63 (= V59), N89 (= N85), A90 (= A86), T140 (= T137), S142 (= S139), Y155 (= Y153), K159 (= K157), A186 (≠ G184), V187 (≠ P185)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
37% identity, 95% coverage: 5:246/254 of query aligns to 7:240/243 of 4i08A
- active site: G19 (= G17), N113 (= N111), S141 (= S139), Q151 (= Q150), Y154 (= Y153), K158 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G13), S17 (≠ G15), R18 (≠ S16), I20 (= I18), T40 (≠ L38), N62 (≠ D58), V63 (= V59), N89 (= N85), A90 (= A86), G140 (≠ A138), S141 (= S139), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), T189 (= T188)
2cfcA Structural basis for stereo selectivity in the (r)- and (s)- hydroxypropylethane thiosulfonate dehydrogenases (see paper)
38% identity, 94% coverage: 7:244/254 of query aligns to 3:245/250 of 2cfcA
- active site: G13 (= G17), S142 (= S139), Y155 (= Y153), K159 (= K157)
- binding (2-[2-ketopropylthio]ethanesulfonate: F149 (≠ A147), R152 (≠ Q150), Y155 (= Y153), W195 (≠ E193), R196 (≠ L194)
- binding nicotinamide-adenine-dinucleotide: G9 (= G13), S12 (= S16), G13 (= G17), N14 (≠ I18), D33 (= D37), L34 (= L38), A59 (= A57), D60 (= D58), V61 (= V59), N87 (= N85), A88 (= A86), G89 (= G87), I140 (≠ T137), P185 (= P183), G186 (= G184), M187 (≠ P185), I188 (≠ V186), T190 (= T188), P191 (= P189), M192 (≠ L190), T193 (≠ L191)
Q56840 2-(R)-hydroxypropyl-CoM dehydrogenase; R-HPCDH; 2-[(R)-2-hydroxypropylthio]ethanesulfonate dehydrogenase; Aliphatic epoxide carboxylation component III; Epoxide carboxylase component III; RHPCDH1; EC 1.1.1.268 from Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) (see 4 papers)
38% identity, 94% coverage: 7:244/254 of query aligns to 3:245/250 of Q56840
- SGN 12:14 (≠ SGI 16:18) binding NAD(+)
- D33 (= D37) binding NAD(+)
- DV 60:61 (= DV 58:59) binding NAD(+)
- N87 (= N85) binding NAD(+)
- S142 (= S139) mutation to A: Retains weak activity. 120-fold decrease in kcat.; mutation to C: Loss of activity.
- R152 (≠ Q150) binding 2-oxopropyl-coenzyme M; mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- Y155 (= Y153) mutation Y->E,F: Loss of activity.
- K159 (= K157) mutation to A: Loss of activity.
- R179 (= R177) mutation to A: Loss of activity.
- IETPM 188:192 (≠ VNTPL 186:190) binding NAD(+)
- WR 195:196 (≠ EL 193:194) binding 2-oxopropyl-coenzyme M
- R196 (≠ L194) mutation to A: Almost loss of activity with the natural substrate 2-KPC, but does not affect activity with 2-butanone as substrate.
- R203 (≠ A202) mutation to A: Slight decrease in catalytic efficiency.
- R209 (≠ H208) mutation to A: Does not affect catalytic efficiency.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
5en4A Complex of 17-beta-hydroxysteroid dehydrogenase type 14 with inhibitor. (see paper)
39% identity, 88% coverage: 28:250/254 of query aligns to 28:250/251 of 5en4A