SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017730539.1 NCBI__GCF_000346615.1:WP_017730539.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
35% identity, 56% coverage: 30:218/339 of query aligns to 30:218/265 of P07821

query
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P07821

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K50 (= K50) mutation to Q: Lack of activity.
D172 (= D172) mutation to E: Lack of activity.
E173 (= E173) mutation to A: Lack of activity.

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
33% identity, 58% coverage: 17:211/339 of query aligns to 13:206/226 of 5xu1B

query
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5xu1B

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binding magnesium ion: G45 (= G49), K46 (= K50), S47 (= S51)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 63% coverage: 16:227/339 of query aligns to 22:230/378 of P69874

query
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P69874

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C26 (≠ K20) mutation to A: Lower ATPase activity and transport efficiency.
F27 (≠ K21) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ L39) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A48) mutation to T: Loss of ATPase activity and transport.
L60 (= L54) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ M70) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ L135) mutation to M: Loss of ATPase activity and transport.
D172 (= D172) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
28% identity, 75% coverage: 8:260/339 of query aligns to 5:257/257 of 8wm7D

query
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8wm7D

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binding adenosine-5'-diphosphate: Y14 (= Y17), Y21 (≠ K24), V23 (= V26), S43 (≠ N46), G44 (= G47), C45 (≠ A48), G46 (= G49), K47 (= K50), S48 (= S51), T49 (= T52)

8w9mD Cryo-em structure of the cyanobacterial nitrate transporter nrtbcd in complex with atp (see paper)
28% identity, 75% coverage: 8:260/339 of query aligns to 3:255/256 of 8w9mD

query
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8w9mD

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binding adenosine-5'-triphosphate: Y12 (= Y17), H40 (≠ P45), S41 (≠ N46), G42 (= G47), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52), Q82 (≠ T92), Q135 (≠ E147), S137 (= S149), G139 (= G151), M140 (≠ Q152), H194 (= H206)
binding magnesium ion: S46 (= S51), Q82 (≠ T92)

7mdyC Lolcde nucleotide-bound
32% identity, 60% coverage: 8:211/339 of query aligns to 3:206/226 of 7mdyC

query
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7mdyC

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L

L

A

G

G

G

V

L

Q

D

E

T

P

P

L

T

A

S

G

G

Q

D

V

V

I

I

M

F

E

N

G

G

Q

Q

P

P

I

M

H

S

Q

K

L

L

A

S

Q

S

Q

A

E

A

R

K

A

A

-

E

-

L

-

R

-

N

R

Q

R

K

I

L

S

G

L

F

V

I

L

Y

T
x
Q

E

F

Q

H

L

H

S

L

A

L

G

P

N

D

M

F

N

T

V

A

L

L

E

E

L

N

V

V

A

A

L

M

G

P

R

L

Y

L

P

I

H

-

T

-

G

-

W

-

G

-

G

G

S

K

L

K

S

K

P

P

A

A

D

E

-

I

K

N

E

S

M

R

V

A

L

L

Q

E

A

M

L

L

N

K

D

A

L

V

D

G

I

L

G

D

E

H

L

R

A

A

G

N

R
x
H

K

R

L

P

Y

S

E
|
E

L

L

S
|
S

D
x
G

G
|
G

Q

E

R

R

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

D

N

G

P

Q

R

L

L

M

V

I

L

L

A

D

D

E
|
E

P

P

T

T

A

G

H
x
N

L

L

D

D

L

A

I

R

N

N

R

A

L

D

Q

S

I

I

M

F

R

Q

Q

L

L

L

R

G

L

E

L

L

A

N

L

R

R

L

R

Q

Q

G

K

T

A

A

I

F

L

L

V

V

A

V

T

T

H
|
H

E

D

L

L

E

Q

L

L

A

A

binding adp orthovanadate: Y12 (= Y17), G42 (= G47), S43 (≠ A48), G44 (= G49), K45 (= K50), S46 (= S51), T47 (= T52), Q91 (≠ T92), H138 (≠ R143), E142 (= E147), S144 (= S149), G145 (≠ D150), G146 (= G151), E168 (= E173), N172 (≠ H177), H201 (= H206)
binding magnesium ion: S46 (= S51), Q91 (≠ T92)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
32% identity, 60% coverage: 8:211/339 of query aligns to 3:206/222 of 7arlD

query
sites
7arlD

L

L

Q

Q

A

C

Q

D

Q

N

L

L

S

C

I

K

G

R

Y

Y

R

Q

N

E

K

G

K

S

A

V

D

Q

K

T

I

D

V

V

R

L

A

H

G

N

L

V

D

S

L

F

E

S

V

V

Y

G

Q

E

G

G

E

E

L

M

C

A

L

I

M

V

G

G

P

S

N

S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

L

R

H

T

L

L

L

A

G

G

G

V

L

Q

D

E

T

P

P

L

T

A

S

G

G

Q

D

V

V

I

I

M

F

E

N

G

G

Q

Q

P

P

I

M

H

S

Q

K

L

L

A

S

Q

S

Q

A

E

A

R

K

A

A

-

E

-

L

-

R

-

N

R

Q

R

K

I

L

S

G

L

F

V

I

L

Y

T

Q

E

F

Q

H

L

H

S

L

A

L

G

P

N

D

M

F

N

T

V

A

L

L

E

E

L

N

V

V

A

A

L

M

G

P

R

L

Y

L

P

I

H

-

T

-

G

-

W

-

G

-

G

G

S

K

L

K

S

K

P

P

A

A

D

E

-

I

K

N

E

S

M

R

V

A

L

L

Q

E

A

M

L

L

N

K

D

A

L

V

D

G

I

L

G

D

E

H

L

R

A

A

G

N

R

H

K

R

L

P

Y

S

E

E

L

L

S

S

D

G

G

G

Q

E

R

R

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

D

N

G

P

Q

R

L

L

M

V

I

L

L

A

D

D

E

E

P

P

T

T

A

G

H

N

L

L

D

D

L

A

I

R

N

N

R

A

L

D

Q

S

I

I

M

F

R

Q

Q

L

L

L

R

G

L

E

L

L

A

N

L

R

R

L

R

Q

Q

G

K

T

A

A

I

F

L

L

V

V

A

V

T

T

H

H

E

D

L

L

E

Q

L

L

A

A

binding adenosine-5'-diphosphate: G42 (= G47), G44 (= G49), K45 (= K50), S46 (= S51)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
32% identity, 60% coverage: 8:211/339 of query aligns to 6:209/233 of P75957

query
sites
P75957

L

L

Q

Q

A

C

Q

D

Q

N

L

L

S

C

I

K

G

R

Y

Y

R

Q

N

E

K

G

K

S

A

V

D

Q

K

T

I

D

V

V

R

L

A

H

G

N

L

V

D

S

L

F

E

S

V

V

Y

G

Q

E

G

G

E

E

L

M

C

A

L

I

M

V

G
|
G

P

S

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

R

H

T

L

L

L

A

G

G

G

V

L

Q

D

E

T

P

P

L

T

A

S

G

G

Q

D

V

V

I

I

M

F

E

N

G

G

Q

Q

P

P

I

M

H

S

Q

K

L

L

A

S

Q

S

Q

A

E

A

R

K

A

A

-

E

-

L

-

R

-

N

R

Q

R

K

I

L

S

G

L

F

V

I

L

Y

T

Q

E

F

Q

H

L

H

S

L

A

L

G

P

N

D

M

F

N

T

V

A

L

L

E

E

L

N

V

V

A

A

L

M

G

P

R

L

Y

L

P

I

H

-

T

-

G

-

W

-

G

-

G

G

S

K

L

K

S

K

P

P

A

A

D

E

-

I

K

N

E

S

M

R

V

A

L

L

Q

E

A

M

L

L

N

K

D

A

L

V

D

G

I

L

G

D

E

H

L

R

A

A

G

N

R

H

K

R

L

P

Y

S

E

E

L

L

S

S

D

G

G

G

Q

E

R

R

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

D

N

G

P

Q

R

L

L

M

V

I

L

L

A

D

D

E

E

P

P

T

T

A

G

H

N

L

L

D

D

L

A

I

R

N

N

R

A

L

D

Q

S

I

I

M

F

R

Q

Q

L

L

L

R

G

L

E

L

L

A

N

L

R

R

L

R

Q

Q

G

K

T

A

A

I

F

L

L

V

V

A

V

T

T

H

H

E

D

L

L

E

Q

L

L

A

A

G42 (= G44) mutation to D: Loss of lipoprotein release when overexpressed.

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
33% identity, 57% coverage: 18:211/339 of query aligns to 5:202/223 of 2pclA

query
sites
2pclA

R

R

N

A

K

E

K

N

A

I

D

K

K

K

I

V

V

I

R

R

A

G

-

Y

-

E

-

I

-

L

-

K

G

G

L

I

D

S

L

L

E

S

V

V

Y

K

Q

K

G

G

E

E

L

F

L

V

C

S

L

I

M

I

G

G

P

A

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

L

R

Y

T

I

L

L

A

G

G

L

V

L

Q

D

E

A

P

P

L

T

A

E

G

G

Q

K

V

V

I

F

M

L

E

E

G

G

Q

K

P

E

I

V

H

D

Q

Y

L

T

A

N

Q

E

Q

K

E

E

-

L

-

S

-

L

R

R

A

N

R

R

R

K

I

L

S

G

L

F

V

V

L

F

T

Q

E

F

Q

H

L

Y

S

L

A

I

G

P

N

E

M

L

N

T

V

A

L

L

E

E

L

N

V

V

A

I

L

V

G

-

R

-

Y

-

P

P

H

M

T

L

G

K

W

M

G

G

G

K

S

P

L

K

S

K

P

E

A

A

D

-

K

K

E

E

M

R

V

G

L

E

Q

Y

A

L

L

L

N

S

D

E

L

L

D

G

I

L

G

G

E

D

L

K

A

L

G

S

R

R

K

K

L

P

Y

Y

E

E

L

L

S

S

D

G

G

G

Q

E

R

Q

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

N

D

E

G

P

Q

I

L

L

M

L

I

F

L

A

D

D

E
|
E

P
|
P

T
|
T

A

G

H

N

L

L

D

D

L

S

I

A

N

N

R

T

L

K

Q

R

I

V

M

M

R

-

Q

D

L

I

R

F

L

L

L

K

A

I

L

N

R

E

R

G

Q

G

K

T

A

S

I

I

L

V

V

M

A

V

T
|
T

H

H

E

E

L

R

E

E

L

L

A

A

binding magnesium ion: E165 (= E173), P166 (= P174), T167 (= T175), T196 (= T205)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
32% identity, 60% coverage: 8:211/339 of query aligns to 5:208/229 of 7v8iD

query
sites
7v8iD

L

L

Q

Q

A

C

Q

D

Q

N

L

L

S

C

I

K

G

R

Y

Y

R

Q

N

E

K

G

K

S

A

V

D

Q

K

T

I

D

V
|
V

R

L

A

H

G

N

L

V

D

S

L

F

E

S

V

V

Y

G

Q

E

G

G

E

E

L

M

C

A

L

I

M

V

G

G

P

S

N
x
S

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

H

T

L

L

L

A

G

G

G

V

L

Q

D

E

T

P

P

L

T

A

S

G

G

Q

D

V

V

I

I

M

F

E

N

G

G

Q

Q

P

P

I

M

H

S

Q

K

L

L

A

S

Q

S

Q

A

E

A

R

K

A

A

-

E

-

L

-

R

-

N

R

Q

R

K

I

L

S

G

L

F

V

I

L

Y

T
x
Q

E

F

Q

H

L

H

S

L

A

L

G

P

N

D

M

F

N

T

V

A

L

L

E

E

L

N

V

V

A

A

L

M

G

P

R

L

Y

L

P

I

H

-

T

-

G

-

W

-

G

-

G

G

S

K

L

K

S

K

P

P

A

A

D

E

-

I

K

N

E

S

M

R

V

A

L

L

Q

E

A

M

L

L

N

K

D

A

L

V

D

G

I

L

G

D

E

H

L
x
R

A

A

G

N

R
x
H

K

R

L

P

Y

S

E
|
E

L

L

S
|
S

D

G

G
|
G

Q
x
E

R

R

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

V

Q

N

D

N

G

P

Q

R

L

L

M

V

I

L

L

A

D

D

E

Q

P

P

T

T

A

G

H

N

L

L

D

D

L

A

I

R

N

N

R

A

L

D

Q

S

I

I

M

F

R

Q

Q

L

L

L

R

G

L

E

L

L

A

N

L

R

R

L

R

Q

Q

G

K

T

A

A

I

F

L

L

V

V

A

V

T

T

H
|
H

E

D

L

L

E

Q

L

L

A

A

binding phosphoaminophosphonic acid-adenylate ester: V23 (= V26), S43 (≠ N46), G44 (= G47), G46 (= G49), K47 (= K50), S48 (= S51), T49 (= T52), Q93 (≠ T92), R137 (≠ L140), H140 (≠ R143), E144 (= E147), S146 (= S149), G148 (= G151), E149 (≠ Q152), H203 (= H206)
binding magnesium ion: S48 (= S51), Q93 (≠ T92)

5x40A Structure of a cbio dimer bound with amppcp (see paper)
37% identity, 64% coverage: 4:221/339 of query aligns to 1:213/280 of 5x40A

query
sites
5x40A

V

M

S

T

P

P

T

I

L

L

Q

A

A

A

Q

E

Q

A

L

L

S

T

I

Y

G

A

Y
x
F

R

P

N

G

-

G

-
x
V

K

K

K
x
A

A

L

D

D

K

D

I

-

V

-

R

-

A

-

G

-

L

L

D

S

L

L

E

A

V

V

Y

P

Q

K

G

G

E

E

L

S

L

L

C

A

L

I

M

L

G

G

P

P

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

L

T

H

L

L

A

N

G

G

V

T

Q

L

E

R

P

P

L

Q

A

S

G

G

Q

R

V

V

I

L

M

L

E

G

G

G

Q

T

P

A

I

T

H

G

Q

H

L

S

A

R

Q

K

Q

D

E

L

R

T

A

G

-

W

-

R

R

R

I

V

S

G

L

L

V

V

L

L

-
x
Q

-

D

-

A

T

D

E

D

Q

Q

L

L

S

F

A

A

G

-

N

-

M

T

N

T

V

V

L

F

E

E

L

D

V

V

A

S

L

F

G

G

R

P

Y

L

P

N

H

L

T

-

G

-

W

-

G

-

S

G

L

L

S

S

P

E

A

A

D

E

-

A

K

R

E

A

M

R

V

V

L

E

Q

E

A

A

L

L

N

A

D

A

L

L

D

S

I

I

G

S

E

D

L

L

A

R

G

D

R

R

K

P

L

T

Y
x
H

E
x
M

L
|
L

S
|
S

D

G

G
|
G

Q
|
Q

R

K

Q

R

K

R

A

V

M

A

I

I

A

A

R

G

A

A

L

V

A

A

Q

M

D

R

G

P

Q

E

L

V

M

L

I

L

L

L

D

D

E
x
Q

P

P

T

T

A

A

H

G

L

L

D

D

L

L

I

A

N

G

R

T

L

E

Q

Q

I

L

M

L

R

T

Q

L

L

L

R

R

L

-

A

G

L

L

R

R

R

A

Q

A

K

G

A

M

I

T

L

L

V

V

-

F

A

S

T

T

H
|
H

E

D

L

V

E

E

L

L

A

A

L

A

Q

A

S

L

A

A

D

D

R

R

L

V

W

A

L

L

L

F

binding phosphomethylphosphonic acid adenylate ester: F14 (≠ Y17), V18 (vs. gap), A20 (≠ K21), N40 (= N46), G41 (= G47), G43 (= G49), K44 (= K50), S45 (= S51), T46 (= T52), Q88 (vs. gap), H139 (≠ Y146), M140 (≠ E147), L141 (= L148), S142 (= S149), G144 (= G151), Q145 (= Q152), Q166 (≠ E173), H198 (= H206)
binding magnesium ion: S45 (= S51), Q88 (vs. gap)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
34% identity, 58% coverage: 26:221/339 of query aligns to 17:209/241 of 4u00A

query
sites
4u00A

V
|
V

R

L

A

K

G

G

L

I

D

H

L

L

E

E

V

V

Y

A

Q

P

G

G

E

E

L

K

L

L

C

V

L

I

M

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

R

R

T

T

L

I

A

N

G

R

V

L

Q

E

E

D

P

F

L

Q

A

E

G

G

Q

E

V

V

I

V

M

V

E

D

G

G

Q

L

P

S

I

V

H

K

-

D

Q

D

L

R

A

A

Q

L

Q

R

E

E

R

I

A

R

R

R

R

E

I

V

S

G

L

M

V

V

L

F

T

Q

E

Q

Q

F

L

N

S

L

A

F

G

P

N

H

M

M

N

T

V

V

L

L

E

E

L

N

V

V

A

T

L

L

G

A

R

P

Y

M

P

R

H

V

T

R

G

R

W

W

G

-

S

-

L

-

S

-

P

P

A

R

D

E

K

K

-

A

-

E

E

K

M

K

V

A

L

L

Q

E

A

L

L

L

N

E

D

R

L

V

D

G

I

I

G

L

E

D

L

Q

A

A

G

R

R

K

K

Y

L

P

Y

A

E

Q

L

L

S

S

D

G

G

G

Q

Q

R

Q

Q

Q

K

R

A

V

M

A

I

I

A

A

R

R

A

A

L

L

A

A

Q

M

D

E

G

P

Q

K

L

I

M

M

I

L

L

F

D

D

E

E

P

P

T

T

A

S

H

A

L

L

D

D

-

P

-

E

-

M

L

V

I

G

N

E

R

V

L

L

Q

D

I

V

M

M

R

R

Q

D

L

L

R

A

L

Q

L

G

A

G

L

M

R

-

Q

-

K

-

A

T

I

M

L

V

V

V

A

V

T

T

H

H

E

E

L

M

E

G

L

F

A

A

L

R

Q

E

S

V

A

A

D

D

R

R

L

V

W

V

L

F

L

M

binding adenosine-5'-diphosphate: V17 (= V26), S37 (≠ N46), G38 (= G47), S39 (≠ A48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
29% identity, 59% coverage: 22:221/339 of query aligns to 14:207/369 of P19566

query
sites
P19566

A

G

D

D

K

V

I

V

V

V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

D

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

T

P

R

I

M

H

N

Q

D

L

I

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T

Q

E

S

Q

Y

L

A

S
x
L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D

K

K

K

E

E

M

V

V

M

L

N

Q

Q

A

R

L

V

N

N

D

Q

-

V

-

A

-

E

-

V

L

L

D

Q

I

L

G

A

E

H

L

L

A

L

G

E

R

R

K

K

L

P

Y

K

E

A

L

L

S

S

D

G

G

G

Q

Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

R

L

V

M

F

I

L

L

L

D

D

E

E

P
|
P

T

L

A

S

H

N

L

L

D
|
D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H

H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

L86 (≠ S96) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
P160 (= P174) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
D165 (= D179) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.

Sites not aligning to the query:

306 E→K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.

2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
29% identity, 58% coverage: 25:221/339 of query aligns to 16:206/374 of 2awnB

query
sites
2awnB

I

V

V
|
V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

E

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

K

P

R

I

M

H

N

Q

D

L

T

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T

Q

E

S

Q

Y

L

A

S

L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D

K

K

K

E

E

M

V

V

I

L

N

Q

Q

A

R

L

V

N

N

D

Q

-

V

-

A

-

E

-

V

L

L

D

Q

I

L

G

A

E

H

L

L

A

L

G

D

R

R

K

K

L

P

Y

K

E

A

L

L

S

S

D

G

G

G

Q

Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

S

L

V

M

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

H

N

L

L

D

D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H

H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

binding adenosine-5'-diphosphate: V17 (= V26), S37 (≠ N46), G38 (= G47), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
29% identity, 58% coverage: 25:221/339 of query aligns to 14:204/367 of 1q12A

query
sites
1q12A

I

V

V

V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

E

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

K

P

R

I

M

H

N

Q

D

L

T

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T

Q

E

S

Q

Y

L

A

S

L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D

K

K

K

E

E

M

V

V

I

L

N

Q

Q

A

R

L

V

N

N

D

Q

-

V

-

A

-

E

-

V

L

L

D

Q

I

L

G

A

E

H

L

L

A

L

G

D

R
|
R

K

K

L

P

Y

K

E
x
A

L

L

S
|
S

D

G

G
|
G

Q
|
Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

S

L

V

M

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

H

N

L

L

D

D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H

H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

binding adenosine-5'-triphosphate: S35 (≠ N46), G36 (= G47), C37 (≠ A48), G38 (= G49), K39 (= K50), S40 (= S51), T41 (= T52), R126 (= R143), A130 (≠ E147), S132 (= S149), G134 (= G151), Q135 (= Q152)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 10

P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
29% identity, 58% coverage: 25:221/339 of query aligns to 17:207/371 of P68187

query
sites
P68187

I

V

V

V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

E

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N

S

G

G

A

C

G

G

K

K

S

S

T

T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

K

P

R

I

M

H

N

Q

D

L

T

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T

Q

E

S

Q

Y

L
x
A

S

L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D
x
K

K

K

E

E

M

V

V

I

L

N

Q

Q

A

R

L
x
V

N

N

D

Q

-
x
V

-

A

-
x
E

-

V

L

L

D

Q

I

L

G
x
A

E

H

L

L

A

L

G

D

R

R

K

K

L

P

Y

K

E

A

L

L

S

S

D

G

G
|
G

Q

Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

S

L

V

M

F

I

L

L

L

D
|
D

E

E

P

P

T

L

A

S

H

N

L

L

D

D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H

H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

A85 (≠ L95) mutation to M: Suppressor of EAA loop mutations in MalFG.
K106 (≠ D124) mutation to C: Suppressor of EAA loop mutations in MalFG.
V114 (≠ L132) mutation to C: Suppressor of EAA loop mutations in MalFG.
V117 (vs. gap) mutation to M: Suppressor of EAA loop mutations in MalFG.
E119 (vs. gap) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
A124 (≠ G138) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
G137 (= G151) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
D158 (= D172) mutation to N: Loss of maltose transport but retains ability to repress mal genes.

Sites not aligning to the query:

228 R→C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
267 W→G: Normal maltose transport but constitutive mal gene expression.
278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
340 G→A: Maltose transport is affected but retains ability to interact with MalT.
346 G→S: Normal maltose transport but constitutive mal gene expression.
355 F→Y: Maltose transport is affected but retains ability to interact with MalT.

3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
29% identity, 58% coverage: 25:221/339 of query aligns to 16:206/371 of 3puyA

query
sites
3puyA

I

V

V

V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

E

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

K

P

R

I

M

H

N

Q

D

L

T

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T
x
Q

E

S

Q

Y

L

A

S

L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D

K

K

K

E

E

M

V

V

I

L

N

Q

Q

A

R

L

V

N

N

D

Q

-

V

-

A

-

E

-

V

L

L

D

Q

I

L

G

A

E

H

L

L

A

L

G

D

R
|
R

K

K

L

P

Y

K

E
x
A

L

L

S
|
S

D

G

G
|
G

Q
|
Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

S

L

V

M

F

I

L

L

L

D

D

E
|
E

P

P

T

L

A

S

H

N

L

L

D

D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H
|
H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

binding phosphoaminophosphonic acid-adenylate ester: S37 (≠ N46), G38 (= G47), C39 (≠ A48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), Q81 (≠ T92), R128 (= R143), A132 (≠ E147), S134 (= S149), G136 (= G151), Q137 (= Q152), E158 (= E173), H191 (= H206)
binding magnesium ion: S42 (= S51), Q81 (≠ T92)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 12

3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
29% identity, 58% coverage: 25:221/339 of query aligns to 16:206/371 of 3puxA

query
sites
3puxA

I

V

V

V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

E

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

K

P

R

I

M

H

N

Q

D

L

T

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T
x
Q

E

S

Q

Y

L

A

S

L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D

K

K

K

E

E

M

V

V

I

L

N

Q

Q

A

R

L

V

N

N

D

Q

-

V

-

A

-

E

-

V

L

L

D

Q

I

L

G

A

E

H

L

L

A

L

G

D

R
|
R

K

K

L

P

Y

K

E

A

L

L

S
|
S

D

G

G
|
G

Q
|
Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

S

L

V

M

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

H

N

L

L

D

D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H
|
H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

binding adenosine-5'-diphosphate: G38 (= G47), C39 (≠ A48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (= R143), S134 (= S149), Q137 (= Q152)
binding beryllium trifluoride ion: S37 (≠ N46), G38 (= G47), K41 (= K50), Q81 (≠ T92), S134 (= S149), G136 (= G151), H191 (= H206)
binding magnesium ion: S42 (= S51), Q81 (≠ T92)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
29% identity, 58% coverage: 25:221/339 of query aligns to 16:206/371 of 3puwA

query
sites
3puwA

I

V

V
|
V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

E

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

K

P

R

I

M

H

N

Q

D

L

T

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T
x
Q

E

S

Q

Y

L

A

S

L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D

K

K

K

E

E

M

V

V

I

L

N

Q

Q

A

R

L

V

N

N

D

Q

-

V

-

A

-

E

-

V

L

L

D

Q

I

L

G

A

E

H

L

L

A

L

G

D

R
|
R

K

K

L

P

Y

K

E
x
A

L

L

S
|
S

D
x
G

G
|
G

Q
|
Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

S

L

V

M

F

I

L

L

L

D

D

E
|
E

P

P

T

L

A

S

H

N

L

L

D

D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H
|
H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

binding adenosine-5'-diphosphate: V17 (= V26), G38 (= G47), C39 (≠ A48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (= R143), A132 (≠ E147), S134 (= S149), Q137 (= Q152)
binding tetrafluoroaluminate ion: S37 (≠ N46), G38 (= G47), K41 (= K50), Q81 (≠ T92), S134 (= S149), G135 (≠ D150), G136 (= G151), E158 (= E173), H191 (= H206)
binding magnesium ion: S42 (= S51), Q81 (≠ T92)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
29% identity, 58% coverage: 25:221/339 of query aligns to 16:206/371 of 3puvA

query
sites
3puvA

I

V

V
|
V

R

S

A

K

G

D

L

I

D

N

L

L

E

D

V

I

Y

H

Q

E

G

G

E

E

L

F

L

V

C

V

L

F

M

V

G

G

P

P

N

S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

T
|
T

L

L

R

R

T

M

L

I

A

A

G

G

V

L

Q

E

E

T

P

I

L

T

A

S

G

G

Q

D

V

L

I

F

M

I

E

G

G

E

Q

K

P

R

I

M

H

N

Q

D

L

T

A

P

Q

P

Q

A

E

E

R

R

A

G

R

-

I

V

S

G

L

M

V

V

L

F

T
x
Q

E

S

Q

Y

L

A

S

L

A

Y

G

P

N

H

M

L

N

S

V

V

L

A

E

E

L

-

V

-

A

-

L

-

G

-

R

-

Y

-

P

-

H

N

T

M

G

S

W

F

G

G

G

L

S

K

L

L

S

A

P

G

A

A

D

K

K

K

E

E

M

V

V

I

L

N

Q

Q

A

R

L

V

N

N

D

Q

-

V

-

A

-

E

-

V

L

L

D

Q

I

L

G

A

E

H

L

L

A

L

G

D

R
|
R

K

K

L

P

Y

K

E
x
A

L

L

S
|
S

D

G

G

G

Q
|
Q

R

R

Q

Q

K

R

A

V

M

A

I

I

A

G

R

R

A

T

L

L

A

V

Q

A

D

E

G

P

Q

S

L

V

M

F

I

L

L

L

D

D

E

E

P

P

T

L

A

S

H

N

L

L

D

D

L

A

I

A

N

L

R

R

L

V

Q

Q

I

M

M

R

R

I

Q

E

L

I

R

S

L

R

L

L

A

H

L

K

R

R

R

L

Q

G

K

R

A

T

I

M

L

I

V

Y

A

V

T

T

H

H

E

D

L

Q

E

V

L

E

A

A

L

M

Q

T

S

L

A

A

D

D

R

K

L

I

W

V

L

V

L

L

binding adenosine-5'-diphosphate: V17 (= V26), G38 (= G47), C39 (≠ A48), G40 (= G49), K41 (= K50), S42 (= S51), T43 (= T52), R128 (= R143), A132 (≠ E147), S134 (= S149), Q137 (= Q152)
binding magnesium ion: S42 (= S51), Q81 (≠ T92)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

Query Sequence

>WP_017730539.1 NCBI__GCF_000346615.1:WP_017730539.1
MKKVSPTLQAQQLSIGYRNKKADKIVRAGLDLEVYQGELLCLMGPNGAGKSTLLRTLAGV
QEPLAGQVIMEGQPIHQLAQQERARRISLVLTEQLSAGNMNVLELVALGRYPHTGWGGSL
SPADKEMVLQALNDLDIGELAGRKLYELSDGQRQKAMIARALAQDGQLMILDEPTAHLDL
INRLQIMRQLRLLALRRQKAILVATHELELALQSADRLWLLPQEQEGFLAGMPEDLVLNG
SLAKAFNRSGFHFDLSSGRFVEEVEHCVPVYLEGEGPARHWTEKALQRYGYALSHDKSTP
LSIRITAEHESYHWQLFAPEGEKVADSLEALVKSLMTER

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory