SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017751184.1 NCBI__GCF_000816635.1:WP_017751184.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
38% identity, 96% coverage: 8:247/251 of query aligns to 7:212/216 of Q9X1K8

query
sites
Q9X1K8

G

G

C

Y

N
x
S

G
|
G

R
|
R

M
|
M

G

G

K

Q

V

E

I

I

S

Q

K

K

L

V

A

F

N

S

T

E

I

-

E

K

D

G

V

H

S

E

I

L

V

V

A

L

G

K

I

V

D

D

R

V

N

N

T

-

E

-

K

-

S

-

N

-

Y

-

P

-

I

-

F

-

A

-

S

G

I

V

S

E

D

E

C

L

N

D

I

-

S

S

A

P

D

D

V

V

I

V

L

I

D

D

F

F

S

S

R

S

P

P

D

E

A

A

L

L

D

P

S

K

L

T

I

V

E

D

Y

L

G

C

K

K

E

K

N

Y

N

R

I

A

G

G

I

L

V

V

F

L

C
x
G

T
|
T

G

A

Y

L

S

K

D

E

E

E

Q

H

I

L

K

Q

K

M

I

L

N

R

D

E

L

L

S

S

K

K

I

E

L

V

P

P

I

V

F

V

R

Q

S
x
A

A
x
Y

N
|
N

M
x
F

S

S

I

I

G

G

I

I

N

N

V

V

V

L

N

K

N

R

I

F

L

L

K

S

N

E

I

L

S

V

S

K

L

V

L

L

Y

-

K

E

N

D

F

W

D

D

I

V

E

E

I

I

V

V

E

E

K

T

H

H

N

R

Q

F

K
|
K

V

K

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

I

L

L

A

E

D

S

T

A

I

L

K

G

D

K

T

S

I

V

P

P

D

-

N

-

I

-

E

-

F

-

V

-

N

-

G

-

R

-

E

-

G

-

I

-

K

-

R

-

V

-

H

-

N

-

E

-

I

-

G

-

I

I

H

H

S

S

I

L

R

R

G

V

G

G

S

G

I

V

V

P

G

G

E

D

H

H

E

V

I

V

L

V

F

F

A

G

G

N

Q

I

G

G

E

E

T

T

I

I

E

E

I

I

K

K

H

H

T

R

A

A

I

I

S

S

R

R

E

T

V

V

F

F

A

A

I

I

G

G

A

A

L

L

K

K

A

A

C

A

E

E

F

F

M

L

Y

V

K

G

K

K

N

D

A

P

G

G

L

M

Y

Y

N

S

M

F

D

E

N

E

V

V

I

I

SGRM 9:12 (≠ NGRM 10:13) binding NAD(+)
GTT 71:73 (≠ CTT 84:86) binding NAD(+)
AYNF 95:98 (≠ SANM 108:111) binding NAD(+)
K131 (= K145) binding NAD(+)

1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
38% identity, 96% coverage: 8:247/251 of query aligns to 12:217/218 of 1vm6B

query
sites
1vm6B

G
|
G

C

Y

N
x
S

G
|
G

R
|
R

M
|
M

G

G

K

Q

V

E

I

I

S

Q

K

K

L

V

A

F

N

S

T

E

I

-

E

K

D

G

V

H

S

E

I

L

V

V

A

L

G

K

I

V

D
|
D

R
x
V

N

N

T

-

E

-

K

-

S

-

N

-

Y

-

P

-

I

-

F

-

A

-

S

G

I

V

S

E

D

E

C

L

N

D

I

-

S

S

A

P

D

D

V

V

I

V

L

I

D

D

F
|
F

S
|
S

R
x
S

P

P

D
x
E

A
|
A

L

L

D

P

S

K

L

T

I

V

E

D

Y

L

G

C

K

K

E

K

N

Y

N

R

I

A

G

G

I

L

V

V

F

L

C
x
G

T

T

T
|
T

G

A

Y

L

S

K

D

E

E

E

Q

H

I

L

K

Q

K

M

I

L

N

R

D

E

L

L

S

S

K

K

I

E

L

V

P

P

I

V

F

V

R

Q

S

A

A
x
Y

N
|
N

M
x
F

S

S

I

I

G

G

I

I

N

N

V

V

V

L

N

K

N

R

I

F

L

L

K

S

N

E

I

L

S

V

S

K

L

V

L

L

Y

-

K

E

N

D

F

W

D

D

I

V

E

E

I

I

V

V

E

E

K

T

H
|
H

H

H

N

R

Q

F

K
|
K

V

K

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

I

L

L

A

E

D

S

T

A

I

L

K

G

D

K

T

S

I

V

P

P

D

-

N

-

I

-

E

-

F

-

V

-

N

-

G

-

R

-

E

-

G

-

I

-

K

-

R

-

V

-

H

-

N

-

E

-

I

-

G

-

I

I

H

H

S

S

I

L

R

R

G

V

G

G

S

G

I

V

V

P

G

G

E

D

H

H

E

V

I

V

L

V

F

F

A

G

G

N

Q

I

G

G

E

E

T

T

I

I

E

E

I

I

K

K

H

H

T

R

A

A

I

I

S

S

R

R

E

T

V

V

F
|
F

A

A

I

I

G

G

A

A

L

L

K

K

A

A

C

A

E

E

F

F

M

L

Y

V

K

G

K

K

N

D

A

P

G

G

L

M

Y

Y

N

S

M

F

D

E

N

E

V

V

I

I

active site: H132 (= H141), K136 (= K145)
binding nicotinamide-adenine-dinucleotide: G12 (= G8), S14 (≠ N10), G15 (= G11), R16 (= R12), M17 (= M13), D37 (= D34), V38 (≠ R35), F53 (= F61), S54 (= S62), S55 (≠ R63), E57 (≠ D65), A58 (= A66), G76 (≠ C84), T78 (= T86), Y101 (≠ A109), N102 (= N110), F103 (≠ M111), F192 (= F222)

5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
36% identity, 98% coverage: 1:247/251 of query aligns to 1:264/269 of 5tejB

query
sites
5tejB

M

M

V

V

K

R

I

I

I

A

L

V

S

A

G
|
G

C

A

N

A

G
|
G

R
|
R

M
|
M

G

G

K

R

V

N

I

L

S

V

K

K

L

A

A

A

N

H

T

H

I

N

E

P

D

V

V

A

S

K

I

V

V

A

A

A

G

G

I

S

D
x
E

R
|
R

N

P

T

E

E

S

-

S

-

L

-

V

-

G

-

V

-

D

-

L

-

G

-

E

-

L

-

C

-

G

-

E

-

G

K

K

S

F

N

D

Y

V

P

V

I

V

F

C

A

D

S

D

I

L

S

A

D

K

C

Q

N

I

I

D

S

Q

A

F

D

D

V

V

I

I

L

I

D

D

F
|
F

S
x
T

R

A

P

P

-

A

D
x
S

A

T

L

L

D

N

S

N

L

L

I

A

-

L

-

C

-

Q

E

Q

Y

Y

G

G

K

K

E

S

N

-

I

-

G

-

I

I

V

V

F

I

C
x
G

T

T

T
|
T

G

G

Y

F

S

T

D

E

E

E

Q

Q

I

R

K

E

K

Q

I

I

N

D

D

L

L

V

S

A

K

Q

I

Q

L

V

P

P

I

V

F

V

R

M

S

A

A
x
P

N
|
N

M
x
Y

S

S

I

V

G

G

I

V

N

N

V

L

V

V

N

F

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

C

D

D

I

I

E

E

I

I

V

V

E

E

K

A

H
|
H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

I

L

G

L

M

A

G

D

E

T

A

I

I

K

A

D

G

T

A

I

M

P

G

D

N

N

K

I

L

E

S

-

D

-

V

F

A

V

V

N

Y

G

A

R

R

E

E

G

G

I

I

K

T

-

G

K

E

R

R

V

T

H

K

N

D

E

E

I

I

G

G

I

F

H

A

S

T

I

I

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

V

E

E

I

I

K

T

H

H

T

K

A

A

I

T

S

D

R

R

E

M

V

T

F
|
F

A

A

I

N

G

G

A

A

L

V

K

K

A

A

C

A

E

V

F

W

M

L

Y

H

K

E

K

K

N

P

A

A

G

G

L

F

Y

Y

N

T

M

M

D

T

N

D

V

V

I

L

active site: H155 (= H141), K159 (= K145)
binding 2,5 Furan Dicarboxylic Acid: T100 (= T86), H156 (= H142), K159 (= K145), S164 (= S150), G165 (= G151), T166 (= T152)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (≠ D34), R35 (= R35), F75 (= F61), T76 (≠ S62), S80 (≠ D65), G98 (≠ C84), T100 (= T86), P123 (≠ A109), N124 (= N110), Y125 (≠ M111), F239 (= F222)

5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
36% identity, 98% coverage: 1:247/251 of query aligns to 1:264/269 of 5tejA

query
sites
5tejA

M

M

V

V

K

R

I

I

I

A

L

V

S

A

G
|
G

C

A

N

A

G
|
G

R
|
R

M
|
M

G

G

K

R

V

N

I

L

S

V

K

K

L

A

A

A

N

H

T

H

I

N

E

P

D

V

V

A

S

K

I

V

V

A

A

A

G

G

I

S

D
x
E

R
|
R

N

P

T

E

E

S

-

S

-

L

-

V

-

G

-

V

-

D

-

L

-

G

-

E

-

L

-

C

-

G

-

E

-

G

K

K

S

F

N

D

Y

V

P

V

I

V

F

C

A

D

S

D

I

L

S

A

D

K

C

Q

N

I

I

D

S

Q

A

F

D

D

V

V

I

I

L

I

D

D

F
|
F

S
x
T

R

A

P

P

-

A

D
x
S

A

T

L

L

D

N

S

N

L

L

I

A

-

L

-

C

-

Q

E

Q

Y

Y

G

G

K

K

E

S

N

-

I

-

G

-

I

I

V

V

F

I

C
x
G

T

T

T
|
T

G

G

Y

F

S

T

D

E

E

E

Q

Q

I

R

K

E

K

Q

I

I

N

D

D

L

L

V

S

A

K

Q

I

Q

L

V

P

P

I

V

F

V

R

M

S

A

A
x
P

N

N

M

Y

S

S

I

V

G

G

I

V

N

N

V

L

V

V

N

F

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

C

D

D

I

I

E

E

I

I

V

V

E

E

K

A

H
|
H

H

H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

I

L

G

L

M

A

G

D

E

T

A

I

I

K

A

D

G

T

A

I

M

P

G

D

N

N

K

I

L

E

S

-

D

-

V

F

A

V

V

N

Y

G

A

R

R

E

E

G

G

I

I

K

T

-

G

K

E

R

R

V

T

H

K

N

D

E

E

I

I

G

G

I

F

H

A

S

T

I

I

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

V

E

E

I

I

K

T

H

H

T

K

A

A

I

T

S

D

R

R

E

M

V

T

F

F

A

A

I

N

G

G

A

A

L

V

K

K

A

A

C

A

E

V

F

W

M

L

Y

H

K

E

K

K

N

P

A

A

G

G

L

F

Y

Y

N

T

M

M

D

T

N

D

V

V

I

L

active site: H155 (= H141), K159 (= K145)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (≠ D34), R35 (= R35), F75 (= F61), T76 (≠ S62), S80 (≠ D65), G98 (≠ C84), T100 (= T86), P123 (≠ A109)

5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
36% identity, 98% coverage: 1:247/251 of query aligns to 1:264/266 of 5temA

query
sites
5temA

M

M

V

V

K

R

I

I

I

A

L

V

S

A

G
|
G

C

A

N

A

G
|
G

R
|
R

M
|
M

G

G

K

R

V

N

I

L

S

V

K

K

L

A

A

A

N

H

T

H

I

N

E

P

D

V

V

A

S

K

I

V

V

A

A

A

G

G

I

S

D
x
E

R
|
R

N

P

T

E

E

S

-

S

-

L

-

V

-

G

-

V

-

D

-

L

-

G

-

E

-

L

-

C

-

G

-

E

-

G

K

K

S

F

N

D

Y

V

P

V

I

V

F

C

A

D

S

D

I

L

S

A

D

K

C

Q

N

I

I

D

S

Q

A

F

D

D

V

V

I

I

L

I

D

D

F
|
F

S
x
T

R

A

P

P

-

A

D
x
S

A

T

L

L

D

N

S

N

L

L

I

A

-

L

-

C

-

Q

E

Q

Y

Y

G

G

K

K

E

S

N

-

I

-

G

-

I

I

V

V

F

I

C
x
G

T

T

T
|
T

G

G

Y

F

S

T

D

E

E

E

Q

Q

I

R

K

E

K

Q

I

I

N

D

D

L

L

V

S

A

K

Q

I

Q

L

V

P

P

I

V

F

V

R

M

S

A

A
x
P

N
|
N

M
x
Y

S

S

I

V

G

G

I

V

N

N

V

L

V

V

N

F

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

C

D

D

I

I

E

E

I

I

V

V

E

E

K

A

H
|
H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

I

L

G

L

M

A

G

D

E

T

A

I

I

K

A

D

G

T

A

I

M

P

G

D

N

N

K

I

L

E

S

-

D

-

V

F

A

V

V

N

Y

G

A

R

R

E

E

G

G

I

I

K

T

-

G

K

E

R

R

V

T

H

K

N

D

E

E

I

I

G

G

I

F

H

A

S

T

I

I

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

V

E

E

I

I

K

T

H

H

T

K

A

A

I

T

S

D

R

R

E

M

V

T

F
|
F

A

A

I

N

G

G

A

A

L

V

K

K

A

A

C

A

E

V

F

W

M

L

Y

H

K

E

K

K

N

P

A

A

G

G

L

F

Y

Y

N

T

M

M

D

T

N

D

V

V

I

L

active site: H155 (= H141), K159 (= K145)
binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (≠ D34), R35 (= R35), F75 (= F61), T76 (≠ S62), S80 (≠ D65), G98 (≠ C84), T100 (= T86), P123 (≠ A109), N124 (= N110), Y125 (≠ M111), F239 (= F222)
binding pyridine-2,6-dicarboxylic acid: T100 (= T86), P123 (≠ A109), H156 (= H142), K159 (= K145), S164 (= S150), G165 (= G151), T166 (= T152)

4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
32% identity, 99% coverage: 1:248/251 of query aligns to 1:266/268 of 4ywjA

query
sites
4ywjA

M

M

V

R

K

R

I

I

I

A

L

V

S

V

G

G

C

A

N

A

G
|
G

R
|
R

M
|
M

G

G

K

K

-

N

V

L

I

I

S

E

K

A

L

V

A

Q

N

Q

T

T

I

G

E

G

D

A

V

A

S

G

I

L

V

T

A

A

G

A

I

V

D
|
D

R
|
R

-

P

-

D

-

S

-

T

-

L

-

V

-

G

-

A

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

L

E

G

K

R

S

I

N

G

Y

V

P

P

I

L

F

S

A

G

S

D

I

L

S

G

D

K

C

V

N

C

I

E

S

E

A

F

D

D

V

V

I

L

L

I

D

D

F
|
F

S
x
T

R

H

P

P

D

S

A
x
V

L

T

D

L

S

K

L

N

I

I

E

E

Y

Q

G

C

K

R

E

K

N

A

N

R

I

R

G

A

I

M

V

V

F

I

C
x
G

T

T

T
|
T

G

G

Y

F

S

S

D

A

E

D

Q

E

I

K

K

L

I

L

N

A

D

E

L

A

S

A

K

K

I

D

L

I

P

P

I

I

F

V

R

F

S

A

A
|
A

N
|
N

M
x
F

S

S

I

V

G

G

I

V

N

N

V

L

V

C

N

L

N

K

I

L

L

L

K

D

N

T

I

A

S

A

S

R

L

V

L

L

Y

G

K

D

N

E

F

V

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

H

H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

L

L

R

L

M

A

G

D

E

T

V

I

V

K

A

D

Q

T

A

I

L

P

G

D

R

N

D

I

L

E

Q

-

E

-

V

F

A

V

V

N

Y

G

G

R

R

E

E

G

G

-

Q

I

T

K

G

K

A

R

R

V

A

H

R

N

E

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

V

V

V

G

G

E

D

H

H

E

T

I

V

L

L

F

F

A

A

G

A

Q

E

G

G

E

E

T

R

I

V

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R
|
R

E

M

V

T

F
|
F

A

A

I

R

G

G

A

A

L

V

K

R

A

A

C

A

E

L

F

W

M

L

Y

E

K

G

K

K

N

E

A

N

G

G

L

L

Y

Y

N

D

M

M

D

Q

N

D

V

V

I

L

S

G

active site: H156 (= H141), K160 (= K145)
binding nicotinamide-adenine-dinucleotide: G11 (= G11), R12 (= R12), M13 (= M13), D35 (= D34), R36 (= R35), F76 (= F61), T77 (≠ S62), V81 (≠ A66), G99 (≠ C84), T101 (= T86), A124 (= A109), N125 (= N110), F126 (≠ M111), R237 (= R219), F240 (= F222)

3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
36% identity, 98% coverage: 2:247/251 of query aligns to 2:264/266 of 3ijpA

query
sites
3ijpA

V

M

K

R

I

L

I

T

L

V

S

V

G
|
G

C

A

N
|
N

G
|
G

R
|
R

M
|
M

G

G

K

R

V

E

I

L

S

I

K

T

L

A

A
x
I

N
x
Q

T

R

I
x
R

E

K

D

D

V
|
V

S

E

I

L

V

C

A

A

G

V

I

L

D

V

R
|
R

N

K

-

G

-

S

-

F

T

V

E

D

K

K

S

D

N

A

Y

S

P

I

I

L

F

I

A

G

S

S

-

D

-

F

-

L

-

G

-

V

-

R

I

I

S

T

D

D

C

D

N

P

I

E

S

S

A

A

-

F

-

S

-

N

-

T

D

E

V

G

I

I

L

L

D

D

F
|
F

S
|
S

R
x
Q

P

P

D

Q

A
|
A

L

S

D

V

S

L

L

Y

I

A

E

N

Y

Y

G

A

K

A

E

Q

N

K

N

S

I

L

G

I

I

H

V

I

F

I

C
x
G

T

T

T
|
T

G

G

Y

F

S

S

D

K

E

T

Q

E

I

E

K

A

K

Q

I

I

N

A

D

D

L

F

S

A

K

K

I

Y

L

T

P

T

I

I

F

V

R

K

S

S

A
x
G

N
|
N

M
|
M

S

S

I

L

G

G

I

V

N

N

V

L

N

A

N

N

I

L

L

V

K

K

N

R

I

A

S

A

S

K

L

A

L

L

Y

D

K

D

N

D

F

F

D

D

I

I

E

E

I

I

V

Y

E

E

K

M

H
|
H

H

H

N

A

Q

N

K
|
K

V

V

D

D

S

S

P

P

S

S

G

G

T

T

A

A

I

L

L

L

A

G

D

Q

T

A

I

A

-

A

-

E

-

G

K

R

D

N

T

I

I

M

P

L

D

K

N

N

I

V

E

S

F

-

V

V

N

N

G

G

R

R

E

S

G

G

-

H

I

T

K

G

K

K

R

R

V

E

H

K

N

G

E

T

I

I

G

G

I

F

H

A

S

C

I

S

R

R

G

G

S

T

I

V

V

I

G

G

E

D

H

H

E

S

I

I

L

T

F

F

A

A

G

G

Q

E

G

N

E

E

T

R

I

I

E

V

I

L

K

S

H

H

T

I

A

A

I

Q

S

E

R

R

E

S

V

I

F
|
F

A

A

I

N

G

G

A

A

L

L

K

K

A

A

C

A

E

L

F

W

M

A

Y

K

K

N

K

H

N

E

A

N

G

G

L

L

Y

Y

N

S

M

M

D

L

N

D

V

V

I

L

active site: H155 (= H141), K159 (= K145)
binding sodium ion: I21 (≠ A21), Q22 (≠ N22), R24 (≠ I24), V27 (= V27)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), N10 (= N10), G11 (= G11), R12 (= R12), M13 (= M13), R35 (= R35), F75 (= F61), S76 (= S62), Q77 (≠ R63), A80 (= A66), G98 (≠ C84), T100 (= T86), G123 (≠ A109), N124 (= N110), M125 (= M111), F239 (= F222)

3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
36% identity, 98% coverage: 2:247/251 of query aligns to 2:264/267 of 3ijpB

query
sites
3ijpB

V

M

K

R

I

L

I

T

L

V

S

V

G

G

C

A

N

N

G

G

R

R

M

M

G

G

K

R

V

E

I

L

S

I

K

T

L

A

A
x
I

N
x
Q

T

R

I
x
R

E

K

D

D

V
|
V

S
x
E

I

L

V

C

A

A

G

V

I

L

D

V

R

R

N

K

-

G

-

S

-

F

T

V

E

D

K

K

S

D

N

A

Y

S

P

I

I

L

F

I

A

G

S

S

-

D

-

F

-

L

-

G

-

V

-

R

I

I

S

T

D

D

C

D

N

P

I

E

S

S

A

A

-

F

-

S

-

N

-

T

D

E

V

G

I

I

L

L

D

D

F

F

S

S

R

Q

P

P

D

Q

A

A

L

S

D

V

S

L

L

Y

I

A

E

N

Y

Y

G

A

K

A

E

Q

N

K

N

S

I

L

G

I

I

H

V

I

F

I

C

G

T

T

G

G

Y

F

S

S

D

K

E

T

Q

E

I

E

K

A

K

Q

I

I

N

A

D

D

L

F

S

A

K

K

I

Y

L

T

P

T

I

I

F

V

R

K

S

S

A

G

N

N

M

M

S

S

I

L

G

G

I

V

N

N

V

L

N

A

N

N

I

L

L

V

K

K

N

R

I

A

S

A

S

K

L

A

L

L

Y

D

K

D

N

D

F

F

D

D

I

I

E

E

I

I

V

Y

E

E

K

M

H
|
H

H

H

N

A

Q

N

K
|
K

V

V

D

D

S

S

P

P

S

S

G

G

T

T

A

A

I

L

L

L

A

G

D

Q

T

A

I

A

-

A

-

E

-

G

K

R

D

N

T

I

I

M

P

L

D

K

N

N

I

V

E

S

F

-

V

V

N

N

G

G

R

R

E

S

G

G

-

H

I

T

K

G

K

K

R

R

V

E

H

K

N

G

E

T

I

I

G

G

I

F

H

A

S

C

I

S

R

R

G

G

S

T

I

V

V

I

G

G

E

D

H

H

E

S

I

I

L

T

F

F

A

A

G

G

Q

E

G

N

E

E

T

R

I

I

E

V

I

L

K

S

H

H

T

I

A

A

I

Q

S

E

R

R

E

S

V

I

F

F

A

A

I

N

G

G

A

A

L

L

K

K

A

A

C

A

E

L

F

W

M

A

Y

K

K

N

K

H

N

E

A

N

G

G

L

L

Y

Y

N

S

M

M

D

L

N

D

V

V

I

L

active site: H155 (= H141), K159 (= K145)
binding sodium ion: I21 (≠ A21), Q22 (≠ N22), R24 (≠ I24), V27 (= V27), E28 (≠ S28)

P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 100% coverage: 2:251/251 of query aligns to 6:272/273 of P04036

query
sites
P04036

V

I

K

R

I

V

I

A

L

I

S

A

G
|
G

C

A

N

G

G
|
G

R
|
R

M
|
M

G

G

K

R

V

Q

I

L

S

I

K

Q

L

A

A

A

N

L

T

A

I

L

E

E

D

G

V

V

S

Q

I

L

V

G

A

A

G

A

I

L

D
x
E

R
|
R

-

E

-

G

-

S

-

L

-

G

-

S

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

A

E

G

K

K

S

T

N

G

Y

V

P

T

I

V

F

Q

A

S

S

S

I

L

S

D

D

A

C

V

N

K

I

D

S

D

A

F

D

D

V

V

I

F

L

I

D

D

F

F

S
x
T

R
|
R

P

P

D

E

A

G

L

T

D

L

S

N

L

H

I

L

E

A

Y

F

G

C

K

R

E

Q

N

H

N

G

I

K

G

G

I

M

V

V

F

I

C
x
G

T
|
T

G

G

Y

F

S

D

D

E

E

A

Q

G

I

K

K

Q

K

A

I

I

N

R

D

D

L

A

S

A

K

A

I

D

L

I

P

A

I

I

F

V

R

F

S
x
A

A
|
A

N
|
N

M
x
F

S

S

I

V

G

G

I

V

N

N

V

V

V

M

N

L

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

T

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

H

H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

L

L

A

L

M

A

G

D

E

T

A

I

I

K

A

D

H

T

A

I

L

P

D

D

K

N

D

I

L

E

K

-

D

-

C

F

A

V

V

N

Y

G

S

R

R

E

E

G

G

-

H

I

T

K

G

K

E

R

R

V

V

H

P

N

G

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R
|
R

E

M

V

T

F
|
F

A

A

I

N

G

G

A

A

L

V

K

R

A

S

C

A

E

L

F

W

M

L

Y

S

K

G

K

K

N

E

A

S

G

G

L

L

Y

F

N

D

M

M

D

R

N

D

V

V

I

L

S

D

N

L

D

N

N

N

G12 (= G8) binding NADP(+)
GRM 15:17 (= GRM 11:13) binding NAD(+)
RM 16:17 (= RM 12:13) binding NADP(+)
E38 (≠ D34) binding NAD(+)
R39 (= R35) binding NADP(+)
TR 80:81 (≠ SR 62:63) binding NAD(+)
GTT 102:104 (≠ CTT 84:86) binding NAD(+); binding NADP(+)
AANF 126:129 (≠ SANM 108:111) binding NAD(+)
F129 (≠ M111) binding NADP(+)
H159 (= H141) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
K163 (= K145) binding NAD(+); mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
R240 (= R219) binding NADP(+)
F243 (= F222) binding NAD(+)

1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
31% identity, 100% coverage: 2:251/251 of query aligns to 2:268/269 of 1arzB

query
sites
1arzB

V

I

K

R

I

V

I

A

L

I

S

A

G
|
G

C

A

N
x
G

G
|
G

R
|
R

M
|
M

G

G

K

R

V

Q

I

L

S

I

K

Q

L

A

A

A

N

L

T

A

I

L

E

E

D

G

V

V

S

Q

I

L

V

G

A

A

G

A

I

L

D
x
E

R

R

-

E

-

G

-

S

-

L

-

G

-

S

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

A

E

G

K

K

S

T

N

G

Y

V

P

T

I

V

F

Q

A

S

S

S

I

L

S

D

D

A

C

V

N

K

I

D

S

D

A

F

D

D

V

V

I

F

L

I

D

D

F
|
F

S
x
T

R
|
R

P

P

D

E

A
x
G

L

T

D

L

S

N

L
x
H

I

L

E

A

Y

F

G

C

K

R

E

Q

N

H

N

G

I

K

G

G

I

M

V

V

F

I

C
x
G

T

T

T
|
T

G

G

Y

F

S

D

D

E

E

A

Q

G

I

K

K

Q

K

A

I

I

N

R

D

D

L

A

S

A

K

A

I

D

L

I

P

A

I

I

F

V

R

F

S

A

A
|
A

N
|
N

M
x
F

S

S

I

V

G

G

I

V

N

N

V

V

V

M

N

L

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

T

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

L

L

A

L

M

A

G

D

E

T

A

I

I

K

A

D

H

T

A

I

L

P

D

D

K

N

D

I

L

E

K

-

D

-

C

F

A

V

V

N

Y

G

S

R

R

E

E

G

G

-

H

I

T

K

G

K

E

R

R

V

V

H

P

N

G

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R

R

E

M

V

T

F
|
F

A

A

I

N

G

G

A

A

L

V

K

R

A

S

C

A

E

L

F

W

M

L

Y

S

K

G

K

K

N

E

A

S

G

G

L

L

Y

F

N

D

M

M

D

R

N

D

V

V

I

L

S

D

N

L

D

N

N

N

active site: H155 (= H141), K159 (= K145)
binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G8), G10 (≠ N10), G11 (= G11), R12 (= R12), M13 (= M13), E34 (≠ D34), F75 (= F61), T76 (≠ S62), R77 (= R63), G80 (≠ A66), H84 (≠ L70), G98 (≠ C84), T100 (= T86), A123 (= A109), N124 (= N110), F125 (≠ M111), F239 (= F222)
binding pyridine-2,6-dicarboxylic acid: T100 (= T86), H156 (= H142), K159 (= K145), S164 (= S150), G165 (= G151), T166 (= T152), F239 (= F222)

1drwA Escherichia coli dhpr/nhdh complex (see paper)
31% identity, 100% coverage: 2:251/251 of query aligns to 5:271/272 of 1drwA

query
sites
1drwA

V

I

K

R

I

V

I

A

L

I

S

A

G
|
G

C

A

N

G

G
|
G

R
|
R

M
|
M

G

G

K

R

V

Q

I

L

S

I

K

Q

L

A

A

A

N

L

T

A

I

L

E

E

D

G

V

V

S

Q

I

L

V

G

A

A

G

A

I

L

D
x
E

R
|
R

-

E

-

G

-

S

-

L

-

G

-

S

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

A

E

G

K

K

S

T

N

G

Y

V

P

T

I

V

F

Q

A

S

S

S

I

L

S

D

D

A

C

V

N

K

I

D

S

D

A

F

D

D

V

V

I

F

L

I

D

D

F
|
F

S
x
T

R
|
R

P

P

D

E

A

G

L

T

D

L

S

N

L

H

I

L

E

A

Y

F

G

C

K

R

E

Q

N

H

N

G

I

K

G

G

I

M

V

V

F

I

C
x
G

T
|
T

G

G

Y

F

S

D

D

E

E

A

Q

G

I

K

K

Q

K

A

I

I

N

R

D

D

L

A

S

A

K

A

I

D

L

I

P

A

I

I

F

V

R

F

S

A

A
|
A

N
|
N

M
x
F

S

S

I

V

G

G

I

V

N

N

V

V

V

M

N

L

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

T

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

H

H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

L

L

A

L

M

A

G

D

E

T

A

I

I

K

A

D

H

T

A

I

L

P

D

D

K

N

D

I

L

E

K

-

D

-

C

F

A

V

V

N

Y

G

S

R

R

E

E

G

G

-

H

I

T

K

G

K

E

R

R

V

V

H

P

N

G

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R

R

E

M

V

T

F
|
F

A

A

I

N

G

G

A

A

L

V

K

R

A

S

C

A

E

L

F

W

M

L

Y

S

K

G

K

K

N

E

A

S

G

G

L

L

Y

F

N

D

M

M

D

R

N

D

V

V

I

L

S

D

N

L

D

N

N

N

active site: H158 (= H141), K162 (= K145)
binding nicotinamide purin-6-ol-dinucleotide: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), E37 (≠ D34), R38 (= R35), F78 (= F61), T79 (≠ S62), R80 (= R63), G101 (≠ C84), T102 (= T85), T103 (= T86), A126 (= A109), N127 (= N110), F128 (≠ M111), F242 (= F222)

1drvA Escherichia coli dhpr/acnadh complex (see paper)
31% identity, 100% coverage: 2:251/251 of query aligns to 3:269/270 of 1drvA

query
sites
1drvA

V

I

K

R

I

V

I

A

L

I

S

A

G
|
G

C

A

N

G

G
|
G

R
|
R

M
|
M

G

G

K

R

V

Q

I

L

S

I

K

Q

L

A

A

A

N

L

T

A

I

L

E

E

D

G

V

V

S

Q

I

L

V

G

A

A

G

A

I

L

D
x
E

R

R

-

E

-

G

-

S

-

L

-

G

-

S

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

A

E

G

K

K

S

T

N

G

Y

V

P

T

I

V

F

Q

A

S

S

S

I

L

S

D

D

A

C

V

N

K

I

D

S

D

A

F

D

D

V

V

I

F

L

I

D

D

F
|
F

S
x
T

R
|
R

P

P

D

E

A
x
G

L

T

D

L

S

N

L

H

I

L

E

A

Y

F

G

C

K

R

E

Q

N

H

N

G

I

K

G

G

I

M

V

V

F

I

C
x
G

T

T

G

G

Y

F

S

D

D

E

E

A

Q

G

I

K

K

Q

K

A

I

I

N

R

D

D

L

A

S

A

K

A

I

D

L

I

P

A

I

I

F

V

R

F

S

A

A
|
A

N

N

M
x
F

S

S

I

V

G

G

I

V

N

N

V

V

V

M

N

L

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

T

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

H

H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

L

L

A

L

M

A

G

D

E

T

A

I

I

K

A

D

H

T

A

I

L

P

D

D

K

N

D

I

L

E

K

-

D

-

C

F

A

V

V

N

Y

G

S

R

R

E

E

G

G

-

H

I

T

K

G

K

E

R

R

V

V

H

P

N

G

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R
|
R

E

M

V

T

F

F

A

A

I

N

G

G

A

A

L

V

K

R

A

S

C

A

E

L

F

W

M

L

Y

S

K

G

K

K

N

E

A

S

G

G

L

L

Y

F

N

D

M

M

D

R

N

D

V

V

I

L

S

D

N

L

D

N

N

N

active site: H156 (= H141), K160 (= K145)
binding 3-acetylpyridine adenine dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (≠ D34), F76 (= F61), T77 (≠ S62), R78 (= R63), G81 (≠ A66), G99 (≠ C84), A124 (= A109), F126 (≠ M111), R237 (= R219)

1druA Escherichia coli dhpr/nadh complex (see paper)
31% identity, 100% coverage: 2:251/251 of query aligns to 3:269/270 of 1druA

query
sites
1druA

V

I

K

R

I

V

I

A

L

I

S

A

G
|
G

C

A

N

G

G
|
G

R
|
R

M
|
M

G

G

K

R

V

Q

I

L

S

I

K

Q

L

A

A

A

N

L

T

A

I

L

E

E

D

G

V

V

S

Q

I

L

V

G

A

A

G

A

I

L

D
x
E

R
|
R

-

E

-

G

-

S

-

L

-

G

-

S

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

A

E

G

K

K

S

T

N

G

Y

V

P

T

I

V

F

Q

A

S

S

S

I

L

S

D

D

A

C

V

N

K

I

D

S

D

A

F

D

D

V

V

I

F

L

I

D

D

F
|
F

S
x
T

R
|
R

P

P

D

E

A
x
G

L

T

D

L

S

N

L

H

I

L

E

A

Y

F

G

C

K

R

E

Q

N

H

N

G

I

K

G

G

I

M

V

V

F

I

C
x
G

T
|
T

G

G

Y

F

S

D

D

E

E

A

Q

G

I

K

K

Q

K

A

I

I

N

R

D

D

L

A

S

A

K

A

I

D

L

I

P

A

I

I

F

V

R

F

S

A

A
|
A

N
|
N

M
x
F

S

S

I

V

G

G

I

V

N

N

V

V

V

M

N

L

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

T

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

H

H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

L

L

A

L

M

A

G

D

E

T

A

I

I

K

A

D

H

T

A

I

L

P

D

D

K

N

D

I

L

E

K

-

D

-

C

F

A

V

V

N

Y

G

S

R

R

E

E

G

G

-

H

I

T

K

G

K

E

R

R

V

V

H

P

N

G

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R

R

E

M

V

T

F
|
F

A

A

I

N

G

G

A

A

L

V

K

R

A

S

C

A

E

L

F

W

M

L

Y

S

K

G

K

K

N

E

A

S

G

G

L

L

Y

F

N

D

M

M

D

R

N

D

V

V

I

L

S

D

N

L

D

N

N

N

active site: H156 (= H141), K160 (= K145)
binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (≠ D34), R36 (= R35), F76 (= F61), T77 (≠ S62), R78 (= R63), G81 (≠ A66), G99 (≠ C84), T100 (= T85), T101 (= T86), A124 (= A109), N125 (= N110), F126 (≠ M111), F240 (= F222)

1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
31% identity, 100% coverage: 2:251/251 of query aligns to 5:271/272 of 1dihA

query
sites
1dihA

V

I

K

R

I

V

I

A

L

I

S

A

G
|
G

C

A

N

G

G
|
G

R
|
R

M
|
M

G

G

K

R

V

Q

I

L

S

I

K

Q

L

A

A

A

N

L

T

A

I

L

E

E

D

G

V

V

S

Q

I

L

V

G

A

A

G

A

I

L

D

E

R
|
R

-

E

-

G

-

S

-

L

-

G

-

S

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

A

E

G

K

K

S

T

N

G

Y

V

P

T

I

V

F

Q

A

S

S

S

I

L

S

D

D

A

C

V

N

K

I

D

S

D

A

F

D

D

V

V

I

F

L

I

D

D

F
|
F

S
x
T

R
|
R

P

P

D

E

A
x
G

L

T

D

L

S

N

L

H

I

L

E

A

Y

F

G

C

K

R

E

Q

N

H

N

G

I

K

G

G

I

M

V

V

F

I

C
x
G

T

T

T
|
T

G

G

Y

F

S

D

D

E

E

A

Q

G

I

K

K

Q

K

A

I

I

N

R

D

D

L

A

S

A

K

A

I

D

L

I

P

A

I

I

F

V

R

F

S

A

A

A

N
|
N

M
x
F

S

S

I

V

G

G

I

V

N

N

V

V

V

M

N

L

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

T

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

H

H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

L

L

A

L

M

A

G

D

E

T

A

I

I

K

A

D

H

T

A

I

L

P

D

D

K

N

D

I

L

E

K

-

D

-

C

F

A

V

V

N

Y

G

S

R

R

E

E

G

G

-

H

I

T

K

G

K

E

R

R

V

V

H

P

N

G

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R
|
R

E

M

V

T

F
|
F

A

A

I

N

G

G

A

A

L

V

K

R

A

S

C

A

E

L

F

W

M

L

Y

S

K

G

K

K

N

E

A

S

G

G

L

L

Y

F

N

D

M

M

D

R

N

D

V

V

I

L

S

D

N

L

D

N

N

N

active site: H158 (= H141), K162 (= K145)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), R38 (= R35), F78 (= F61), T79 (≠ S62), R80 (= R63), G83 (≠ A66), G101 (≠ C84), T103 (= T86), N127 (= N110), F128 (≠ M111), R239 (= R219), F242 (= F222)

1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
31% identity, 100% coverage: 2:251/251 of query aligns to 3:269/270 of 1arzA

query
sites
1arzA

V

I

K

R

I

V

I

A

L

I

S

A

G

G

C

A

N

G

G

G

R

R

M

M

G

G

K

R

V

Q

I

L

S

I

K

Q

L

A

A

A

N

L

T

A

I

L

E

E

D

G

V

V

S

Q

I

L

V

G

A

A

G

A

I

L

D

E

R

R

-

E

-

G

-

S

-

L

-

G

-

S

-

D

-

A

-

G

-

E

-

L

-

A

N

G

T

A

E

G

K

K

S

T

N

G

Y

V

P

T

I

V

F

Q

A

S

S

S

I

L

S

D

D

A

C

V

N

K

I

D

S

D

A

F

D

D

V

V

I

F

L

I

D

D

F

F

S

T

R

R

P

P

D

E

A

G

L

T

D

L

S

N

L

H

I

L

E

A

Y

F

G

C

K

R

E

Q

N

H

N

G

I

K

G

G

I

M

V

V

F

I

C

G

T

T

G

G

Y

F

S

D

D

E

E

A

Q

G

I

K

K

Q

K

A

I

I

N

R

D

D

L

A

S

A

K

A

I

D

L

I

P

A

I

I

F

V

R

F

S

A

A

A

N

N

M

F

S

S

I

V

G

G

I

V

N

N

V

V

V

M

N

L

N

K

I

L

L

L

K

E

N

K

I

A

S

A

S

K

L

V

L

M

Y

G

K

D

N

Y

F

T

D

D

I

I

E

E

I

I

V

I

E

E

K

A

H
|
H

N

R

Q

H

K
|
K

V

V

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

L

L

A

L

M

A

G

D

E

T

A

I

I

K

A

D

H

T

A

I

L

P

D

D

K

N

D

I

L

E

K

-

D

-

C

F

A

V

V

N

Y

G

S

R

R

E

E

G

G

-

H

I

T

K

G

K

E

R

R

V

V

H

P

N

G

E

T

I

I

G

G

I

F

H

A

S

T

I

V

R

R

G

A

G

G

S

D

I

I

V

V

G

G

E

E

H

H

E

T

I

A

L

M

F

F

A

A

G

D

Q

I

G

G

E

E

T

R

I

L

E

E

I

I

K

T

H

H

T

K

A

A

I

S

S

S

R

R

E

M

V

T

F

F

A

A

I

N

G

G

A

A

L

V

K

R

A

S

C

A

E

L

F

W

M

L

Y

S

K

G

K

K

N

E

A

S

G

G

L

L

Y

F

N

D

M

M

D

R

N

D

V

V

I

L

S

D

N

L

D

N

N

N

active site: H156 (= H141), K160 (= K145)
binding phosphate ion: H157 (= H142), K160 (= K145)

5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 98% coverage: 1:247/251 of query aligns to 2:265/265 of 5z2fA

query
sites
5z2fA

M

M

V

I

K

R

I

V

I

A

L

I

S

G

G

G

C

P

N
x
R

G
|
G

R
x
K

M
|
M

G

G

K

Q

V

E

I

A

S

V

K

H

L

T

A

V

N

M

T

N

I

N

E

E

D

N

V

M

S

E

I

L

V

V

A

A

G

V

I

L

D
|
D

R
x
H

N
x
K

-

D

-

I

-

G

-

D

-

L

T

S

E

E

K

S

S

P

N

N

Y

F

P

P

-

A

-

S

-

Y

-

E

-

V

-

P

I

V

F

F

A

L

S

N

I

L

S

E

D

S

-

L

-

I

C

V

N

T

I

I

S

K

A

P

D

D

V

V

I

F

L

L

D

D

F
x
L

S
x
T

R

T

P

P

D

H

A

Q

L

V

D

F

S

E

L

H

I

T

E

M

Y

L

G

C

K

L

E

Q

N

N

I

V

G

R

I

P

V

V

F

I

C
x
G

T
|
T

G

G

Y

F

S

T

D

D

E

E

Q

Q

I

L

K

Q

I

C

N

T

D

I

L

L

S

A

K

E

I

V

-

N

-

K

L

L

P

G

I

C

F

I

R

V

S

A

A
x
P

N
|
N

M
x
F

S

A

I

I

G

G

I

-

N

-

V

-

N

A

N

V

I

L

L

M

K

M

N

K

I

F

S

A

S

S

L

L

L

A

Y

A

K

A

N

Y

F

F

-

P

D

D

I

V

E

E

I

I

V

I

E

E

K

M

H
|
H

N

D

Q

Q

K
|
K

V

L

D

D

S

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

Y

L

K

L

T

A

A

D

Q

T

M

I

I

K

A

D

E

T

V

I

R

P

P

D

S

N

H

I

K

E

Q

-

G

F

H

V

P

N

N

G

E

R

K

E

E

-

T

-

L

-

E

G

G

I

A

K

R

K

G

R

A

V

S

H

Y

N

D

E

G

I

I

G

P

I

I

H

H

S

S

I

V

R

R

G

L

G

P

S

G

I

L

V

I

G
x
A

E

H

H

Q

E

Q

I

I

L

L

F

F

A

G

G

G

Q

E

G

G

E

Q

T

L

I

F

E

T

I

L

K

R

H

H

T

D

A

S

I

Y

S

N

R

R

E

Q

V

S

F

F

A

M

I

S

G

G

A

V

L

T

K

F

A

S

C

I

E

N

F

Q

M

V

Y

M

K

E

K

I

N

K

A

E

G

L

L

V

Y

Y

N

G

M

L

D

E

N

N

V

I

I

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ N10), G12 (= G11), K13 (≠ R12), M14 (= M13), D35 (= D34), H36 (≠ R35), K37 (≠ N36), L76 (≠ F61), T77 (≠ S62), G99 (≠ C84), T100 (= T85), T101 (= T86), P126 (≠ A109), N127 (= N110), F128 (≠ M111)
binding pyridine-2,6-dicarboxylic acid: P126 (≠ A109), H155 (= H141), H156 (= H142), K159 (= K145), S164 (= S150), G165 (= G151), T166 (= T152), A215 (≠ G197)

5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
27% identity, 98% coverage: 1:247/251 of query aligns to 2:265/265 of 5z2eA

query
sites
5z2eA

M

M

V

I

K

R

I

V

I

A

L

I

S

G

G

G

C

P

N

R

G

G

R

K

M

M

G

G

K

Q

V

E

I

A

S

V

K

H

L

T

A

V

N

M

T

N

I

N

E

E

D

N

V

M

S

E

I

L

V

V

A

A

G

V

I

L

D

D

R

H

N

K

-

D

-

I

-

G

-

D

-

L

T

S

E

E

K

S

S

P

N

N

Y

F

P

P

-

A

-

S

-

Y

-

E

-

V

-

P

I

V

F

F

A

L

S

N

I

L

S

E

D

S

-

L

-

I

C

V

N

T

I

I

S

K

A

P

D

D

V

V

I

F

L

L

D

D

F

L

S

T

R

T

P

P

D

H

A

Q

L

V

D

F

S

E

L

H

I

T

E

M

Y

L

G

C

K

L

E

Q

N

N

I

V

G

R

I

P

V

V

F

I

C

G

T

T

G

G

Y

F

S

T

D

D

E

E

Q

Q

I

L

K

Q

I

C

N

T

D

I

L

L

S

A

K

E

I

V

-

N

-

K

L

L

P

G

I

C

F

I

R

V

S

A

A

P

N

N

M

F

S

A

I

I

G

G

I

-

N

-

V

-

N

A

N

V

I

L

L

M

K

M

N

K

I

F

S

A

S

S

L

L

L

A

Y

A

K

A

N

Y

F

F

-

P

D

D

I

V

E

E

I

I

V

I

E

E

K

M

H
|
H

N

D

Q

Q

K
|
K

V

L

D

D

S

A

P

P

S
|
S

G
|
G

T
|
T

A

A

I

Y

L

K

L

T

A

A

D

Q

T

M

I

I

K

A

D

E

T

V

I

R

P

P

D

S

N

H

I

K

E

Q

-

G

F

H

V

P

N

N

G

E

R

K

E

E

-

T

-

L

-

E

G

G

I

A

K

R

K

G

R

A

V

S

H

Y

N

D

E

G

I

I

G

P

I

I

H

H

S

S

I

V

R

R

G

L

G

P

S

G

I

L

V

I

G

A

E

H

H

Q

E

Q

I

I

L

L

F

F

A

G

G

G

Q

E

G

G

E

Q

T

L

I

F

E

T

I

L

K

R

H

H

T

D

A

S

I

Y

S

N

R

R

E

Q

V

S

F

F

A

M

I

S

G

G

A

V

L

T

K

F

A

S

C

I

E

N

F

Q

M

V

Y

M

K

E

K

I

N

K

A

E

G

L

L

V

Y

Y

N

G

M

L

D

E

N

N

V

I

I

L

binding pyridine-2,6-dicarboxylic acid: H155 (= H141), H156 (= H142), K159 (= K145), S164 (= S150), G165 (= G151), T166 (= T152)

5wolA Crystal structure of dihydrodipicolinate reductase dapb from coxiella burnetii
29% identity, 98% coverage: 2:247/251 of query aligns to 2:230/230 of 5wolA

query
sites
5wolA

V

I

K

N

I

V

I

I

L

I

S
x
N

G

G

C

I

N

N

G
|
G

R
x
K

M
|
M

G

G

K

R

V

V

I

V

S

K

K

E

L

N

A

I

N

T

T

A

I

Q

E

S

D

D

V

L

S

E

I

L

V

V

A

S

G

G

I

T

D
x
G

R
|
R

N

Q

T

D

E

D

K

-

S

-

N

-

Y

-

P

-

I

L

F

A

A

K

S

T

I

I

S

Q

D

T

C

T

N

H

I

-

S

-

A

A

D

D

V

V

I

V

L

I

D

D

F
|
F

S
x
T

R
x
T

P

P

D

Q

A
x
S

L

V

-

F

-

H

-

N

-

A

D

E

S

I

L

I

I

I

E

Q

Y

S

G

G

K

A

E

R

N

P

N

V

I

I

G
|
G

I

-

V

-

F

-

C

-

T
|
T

G

G

Y

L

S

T

D

L

E

E

Q

Q

I

I

K

A

K

L

I

L

N

D

D

K

L

Q

-

C

-

R

S

N

K

K

I

K

L

L

P

G

I

A

F

I

R

V

S

A

A
x
P

N
|
N

M
x
F

S

S

I

V

G

G

I

A

N

V

V

L

V

M

N

M

N

K

I

Y

L

A

K

K

N

E

I

A

S

A

S

H

L

Y

L

F

Y

P

K

-

N

-

F

-

D

D

I

V

E

E

I

I

V

I

E

E

K

M

H
|
H

H

H

N

S

Q

Q

K
|
K

V

I

D

D

S

A

P

P

S

S

G

G

T
|
T

A

A

I

I

L
x
K

L

T

A

A

D

Q

T

M

I

I

K

G

D

-

T

-

I

-

P

-

D

-

N

-

I

-

E

E

F

M

V

R

N

S

G

S

R

R

E

A

G

-

I

-

K

R

K

G

R

E

V

I

H

K

N

N

E

G

I

I

G

P

I

I

H

H

S

S

I

I

R

R

G

L

G

P

S

G

I

L

V

F

G

S

E

H

H

Q

E

S

I

V

L

I

F

F

A

G

G

S

Q

N

G

G

E

E

T

T

I

L

E

T

I

I

K

R

H

H

T

D

A

G

I

M

S

D

R

R

E

N

V

C

F

T

A

M

I

P

G

G

A

I

L

F

K

M

A

A

C

C

E

R

F

K

M

V

Y

M

K

E

K

L

N

D

A

Y

G

L

L

V

Y

Y

N

G

M

L

D

E

N

N

V

L

I

L

active site: H133 (= H141), K137 (= K145)
binding pyridine-2-carboxylic acid: P104 (≠ A109), T144 (= T152), K147 (≠ L155)
binding nadp nicotinamide-adenine-dinucleotide phosphate: N7 (≠ S7), G11 (= G11), K12 (≠ R12), M13 (= M13), G34 (≠ D34), R35 (= R35), F54 (= F61), T55 (≠ S62), T56 (≠ R63), S59 (≠ A66), G77 (= G80), T78 (= T85), T79 (= T86), P104 (≠ A109), N105 (= N110), F106 (≠ M111)

1yl5A Crystal structure of mycobacterium tuberculosis dihydrodipicolinate reductase (rv2773c) (crystal form a) (see paper)
26% identity, 94% coverage: 1:236/251 of query aligns to 2:233/247 of 1yl5A

query
sites
1yl5A

M

M

V

A

K

R

I

V

I

G

L

V

S

L

G

G

C

A

N

K

G

G

R

K

M

V

G

G

K

A

V

T

I

M

S

V

K

R

L

A

A
x
V

N
x
A

T

A

I
x
A

E

D

D

D

V
x
L

S

T

I

L

V

S

A

A

G

E

I

L

D

D

R

A

N

G

T

D

E

P

K

L

S

S

N

-

Y

-

P

-

I

-

F

-

A

-

S

L

I

L

S

T

D

D

C

G

N

N

I

T

S

-

A

-

D

E

V

V

I

V

L

I

D

D

F

F

S

T

R

H

P

P

D

D

A

V

L

V

D

M

S

G

L

N

I

L

E

E

Y

F

G

L

K

I

E

D

N

N

N

G

I

I

G

H

I

A

V

V

F

V

C

G

T

T

G

G

Y

F

S

T

D

A

E

E

Q

R

I

F

K

Q

I

V

N

E

D

S

L

W

S

L

K

V

I

A

L

K

P

P

-

N

-

T

-

S

I

V

F

L

R

I

S

A

A

P

N

N

M

F

S

A

I

I

G

G

I

A

N

V

V

L

V

S

N

M

N

H

I

F

L

A

K

K

N

Q

I

A

S

A

S

-

L

-

Y

-

K

R

N

F

F

F

D

D

-

S

I

A

E

E

I

V

V

I

E

E

K

L

H
|
H

H

H

N

P

Q

H

K
|
K

V

A

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

A

-

R

-

T

-

A

-

K

L

L

L

I

A

A

D

E

T

A

I

R

K

K

D

G

T

L

I

P

P

P

D

N

N

P

I

D

E

A

F

T

V

S

N

T

G

S

R

L

E

P

G

G

I

A

K

R

K

G

R

A

V

D

H

V

N

D

E

G

I

I

G

P

I

V

H

H

S

A

I

V

R

R

G

L

G

A

S

G

I

L

V

V

G

A

E

H

H

Q

E

E

I

V

L

L

F

F

A

G

G

T

Q

E

G

G

E

E

T

T

I

L

E

T

I

I

K

R

H

H

T

D

A

S

I

L

S

D

R

R

E

T

V

S

F

F

A

V

I

P

G

G

A

V

L

L

K

L

A

A

C

V

E

R

F

R

M

I

Y

A

K

E

K

R

active site: H134 (= H141), K138 (= K145)
binding magnesium ion: V22 (≠ A21), A23 (≠ N22), A25 (≠ I24), L28 (≠ V27)

P9WP23 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
26% identity, 94% coverage: 2:236/251 of query aligns to 1:231/245 of P9WP23

query
sites
P9WP23

V

M

K

R

I

V

I

G

L

V

S

L

G

G

C

A

N
x
K

G

G

R
x
K

M
x
V

G

G

K

A

V

T

I

M

S

V

K

R

L

A

A

V

N

A

T

A

I

A

E

D

D

D

V

L

S

T

I

L

V

S

A

A

G

E

I

L

D
|
D

R

A

N

G

T

D

E

P

K

L

S

S

N

-

Y

-

P

-

I

-

F

-

A

-

S

L

I

L

S

T

D

D

C

G

N

N

I

T

S

-

A

-

D

E

V

V

I

V

L

I

D

D

F

F

S

T

R

H

P

P

D

D

A

V

L

V

D

M

S

G

L

N

I

L

E

E

Y

F

G

L

K

I

E

D

N

N

N

G

I

I

G

H

I

A

V

V

F

V

C
x
G

T
|
T

G

G

Y

F

S

T

D

A

E

E

Q

R

I

F

K

Q

I

V

N

E

D

S

L

W

S

L

K

V

I

A

L

K

P

P

-

N

-

T

-

S

I

V

F

L

R

I

S
x
A

A
x
P

N
|
N

M
x
F

S

A

I

I

G

G

I

A

N

V

V

L

V

S

N

M

N

H

I

F

L

A

K

K

N

Q

I

A

S

A

S

-

L

-

Y

-

K

R

N

F

F

F

D

D

-

S

I

A

E

E

I

V

V

I

E

E

K

L

H

H

N

P

Q

H

K
|
K

V

A

D

D

S

A

P

P

S

S

G

G

T

T

A

A

I

A

-

R

-

T

-

A

-

K

L

L

L

I

A

A

D

E

T

A

I

R

K

K

D

G

T

L

I

P

P

P

D

N

N

P

I

D

E

A

F

T

V

S

N

T

G

S

R

L

E

P

G

G

I

A

K

R

K

G

R

A

V

D

H

V

N

D

E

G

I

I

G

P

I

V

H

H

S

A

I

V

R

R

G

L

G

A

S

G

I

L

V

V

G

A

E

H

H

Q

E

E

I

V

L

L

F

F

A

G

G

T

Q

E

G

G

E

E

T

T

I

L

E

T

I

I

K

R

H

H

T

D

A

S

I

L

S

D

R

R

E

T

V

S

F

F

A

V

I

P

G

G

A

V

L

L

K

L

A

A

C

V

E

R

F

R

M

I

Y

A

K

E

K

R

K9 (≠ N10) mutation to A: Increases the nucleotide specificity from 6:1 for the wild-type enzyme to 34:1, due to a 4-fold decrease in NADPH affinity while the affinity for NADH remains nearly unchanged.
K11 (≠ R12) mutation to A: 2.8-fold increase in catalytic activity with NADH as substrate, while the affinity for NADH is essentially unaffected. 70-fold decrease in affinity for NADPH, causing the nucleotide specificity to increase from 6:1 for the wild-type enzyme to 187:1.
KV 11:12 (≠ RM 12:13) binding NAD(+); binding NADP(+)
D33 (= D34) binding NAD(+)
GTT 75:77 (≠ CTT 84:86) binding NAD(+); binding NADP(+)
APNF 102:105 (≠ SANM 108:111) binding NAD(+); binding NADP(+)
K136 (= K145) binding NAD(+); binding NADP(+)

Query Sequence

>WP_017751184.1 NCBI__GCF_000816635.1:WP_017751184.1
MVKIILSGCNGRMGKVISKLANTIEDVSIVAGIDRNTEKSNYPIFASISDCNISADVILD
FSRPDALDSLIEYGKENNIGIVFCTTGYSDEQIKKINDLSKILPIFRSANMSIGINVVNN
ILKNISSLLYKNFDIEIVEKHHNQKVDSPSGTAILLADTIKDTIPDNIEFVNGREGIKKR
VHNEIGIHSIRGGSIVGEHEILFAGQGETIEIKHTAISREVFAIGALKACEFMYKKNAGL
YNMDNVISNDN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory