SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017752631.1 NCBI__GCF_000816635.1:WP_017752631.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P18157 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Bacillus subtilis (strain 168) (see paper)
62% identity, 99% coverage: 1:494/497 of query aligns to 1:495/496 of P18157

query
sites
P18157

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F

H230 (≠ S229) mutation to R: Increased activity.
F232 (= F231) mutation to S: Increased activity.

P0A6F3 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Escherichia coli (strain K12) (see 10 papers)
62% identity, 100% coverage: 3:497/497 of query aligns to 5:500/502 of P0A6F3

query
sites
P0A6F3

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H

T14 (= T12) binding ADP; binding sn-glycerol 3-phosphate
R18 (= R16) binding ADP
S59 (≠ G57) mutation to W: Abolishes inhibition of GK by FBP via disruption of the dimer-tetramer assembly reaction. Inhibition by EIIA-Glc is unchanged compared to wild type. The activity of this mutant is significantly higher than wild-type, and the Michaelis constants are increased slightly compared to wild-type.
A66 (≠ L64) mutation to T: Although it completely abolishes FBP regulation and disrupts dimer-tetramer equilibrium, the crystal structure is essentially identical to the symmetric tetramer found in the FBP-bound form of the enzyme.
R84 (= R82) binding glycerol; binding sn-glycerol 3-phosphate
E85 (= E83) binding glycerol; binding sn-glycerol 3-phosphate
Y136 (= Y134) binding glycerol; binding sn-glycerol 3-phosphate
G231 (≠ E228) mutation to D: Displays an increased enzymatic activity and a decreased allosteric regulation by FBP compared to wild-type. It displays a dimer form and is resistant to tetramer formation in the presence of FBP, whereas wild-type dimers are converted into inactive tetramers in the presence of FBP.
K233 (≠ L230) modified: N6-malonyllysine
G235 (= G232) binding beta-D-fructose 1,6-bisphosphate
R237 (≠ E234) binding beta-D-fructose 1,6-bisphosphate; mutation to A: Drastically reduces inhibition of GK by FBP and lowers, but did not eliminate, the ability of FBP to promote tetramer association.
D246 (= D243) binding glycerol; binding sn-glycerol 3-phosphate
Q247 (= Q244) binding glycerol
T268 (= T265) binding ADP
G305 (= G302) mutation to S: In glpK22; abolishes glucose control of glycerol utilization.
G311 (= G308) binding ADP
G412 (= G409) binding ADP
N416 (= N413) binding ADP
I475 (≠ M472) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. It increases the affinity for FBP about fivefold.
E479 (≠ K476) binding Zn(2+)
R480 (= R477) mutation to D: It decreases Vmax to about 10% of the wild-type value and the affinity for substrate is increased about two- to fourfold. This mutation decreases the catalytic activity in a manner that is analogous to that obtained upon EIIA-Glc binding. Regulation by FBP is not affected by this substitution. No inhibition by EIIA-Glc is observed, which is consistent with a decrease in affinity for EIIA-Glc of about 250-fold.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1bu6Y Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 3:498/499 of 1bu6Y

query
sites
1bu6Y

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G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

T

-

N

A

I

E

G

S

G

L

K

F

G

G

G

V

T

E

R

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F

F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G

G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A

A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G

G

A

A

C

V

A

A

N

N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding glycerol: Q81 (= Q81), R82 (= R82), E83 (= E83), W102 (= W102), Y134 (= Y134), D244 (= D243)

1gllO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 3:494/494 of 1gllO

query
sites
1gllO

K

K

Y

Y

V

I

M

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

W

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

-

A

-

E

-

S

T

L

N

F

I

G

G

V

T

E

R

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G
|
G

A

A

S

S

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A
|
A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A

A

C

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding phosphomethylphosphonic acid adenylate ester: T12 (= T12), T13 (= T13), G261 (= G264), T262 (= T265), G305 (= G308), I308 (= I311), Q309 (= Q312), A321 (= A324), G406 (= G409), N410 (= N413)
binding glycerol: R82 (= R82), E83 (= E83), Y134 (= Y134), D240 (= D243), Q241 (= Q244), F265 (= F268)

1gljO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 3:494/494 of 1gljO

query
sites
1gljO

K

K

Y

Y

V

I

M

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

W

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

-

A

-

E

-

S

T

L

N

F

I

G

G

V

T

E

R

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G
|
G

A

A

S

S

I

I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A
|
A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

C

V

A

A

N

N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding gamma-arsono-beta, gamma-methyleneadenosine-5'-diphosphate: T12 (= T12), T13 (= T13), G261 (= G264), T262 (= T265), G305 (= G308), Q309 (= Q312), A321 (= A324), G406 (= G409), A407 (= A410)
binding glycerol: R82 (= R82), E83 (= E83), W102 (= W102), Y134 (= Y134), D240 (= D243), F265 (= F268)

1bwfO Escherichia coli glycerol kinase mutant with bound atp analog showing substantial domain motion (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 3:494/494 of 1bwfO

query
sites
1bwfO

K

K

Y

Y

V

I

M

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T
|
T

S

S

S

S

R

R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

W

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

-

A

-

E

-

S

T

L

N

F

I

G

G

V

T

E

R

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G
|
G

A

A

S

S

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A
|
A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A

A

C

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding phosphodifluoromethylphosphonic acid-adenylate ester: T12 (= T12), T13 (= T13), T262 (= T265), G305 (= G308), I308 (= I311), Q309 (= Q312), A321 (= A324), G406 (= G409), N410 (= N413)
binding glycerol: R82 (= R82), E83 (= E83), W102 (= W102), Y134 (= Y134), D240 (= D243), Q241 (= Q244), F265 (= F268)

1glfO Crystal structures of escherichia coli glycerol kinase and the mutant a65t in an inactive tetramer: conformational changes and implications for allosteric regulation (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 3:498/498 of 1glfO

query
sites
1glfO

K

K

Y

Y

V

I

M

V

A

A

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R
|
R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

S

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

T

-

N

A

I

E

G

S

G

L

K

F
x
G

G
|
G

V

T

E
x
R

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F

F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G
|
G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A

A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

C

V

A

A

N

N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding adenosine-5'-diphosphate: R16 (= R16), G265 (= G264), T266 (= T265), G309 (= G308), G410 (= G409), A411 (= A410)
binding glycerol: R82 (= R82), E83 (= E83), Y134 (= Y134), D244 (= D243)
binding phosphate ion: G232 (≠ F231), G233 (= G232), R235 (≠ E234)

1bo5O Crystal structure of the complex between escherichia coli glycerol kinase and the allosteric regulator fructose 1,6-bisphosphate. (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 3:498/498 of 1bo5O

query
sites
1bo5O

K

K

Y

Y

V

I

M

V

A

A

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

S

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

T

-

N

A

I

E

G

S

G

L

K

F
x
G

G
|
G

V

T

E
x
R

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G

G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A

A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G

G

A

A

C

V

A

A

N

N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding 1,6-di-O-phosphono-beta-D-fructofuranose: G232 (≠ F231), G233 (= G232), R235 (≠ E234)
binding glycerol: R82 (= R82), E83 (= E83), W102 (= W102), Y134 (= Y134), D244 (= D243), Q245 (= Q244), F269 (= F268)

1gldG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 1:489/489 of 1gldG

query
sites
1gldG

K

K

Y

Y

V

I

M

V

A

A

L

L

D
|
D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R
|
R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

S

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

T

A

N

E

I

S

-

L

-

F

-

G

-

V

-

E

-

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G
|
G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A
|
A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

C

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding adenosine-5'-diphosphate: R14 (= R16), G256 (= G264), T257 (= T265), G300 (= G308), A316 (= A324), G401 (= G409), A402 (= A410), N405 (= N413)
binding glyceraldehyde-3-phosphate: T10 (= T12), R80 (= R82), E81 (= E83), Y132 (= Y134), D235 (= D243), F260 (= F268)
binding manganese (ii) ion: D7 (= D9), R14 (= R16)

1glcG Cation promoted association (cpa) of a regulatory and target protein is controlled by phosphorylation (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 1:489/489 of 1glcG

query
sites
1glcG

K

K

Y

Y

V

I

M

V

A

A

L

L

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R

R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

S

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

T

A

N

E

I

S

-

L

-

F

-

G

-

V

-

E

-

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G
|
G

A

A

S

S

I

I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A
|
A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

C

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding adenosine-5'-diphosphate: G256 (= G264), T257 (= T265), G300 (= G308), A316 (= A324), G401 (= G409), A402 (= A410), N405 (= N413)
binding glyceraldehyde-3-phosphate: T10 (= T12), R80 (= R82), E81 (= E83), W100 (= W102), Y132 (= Y134), D235 (= D243), F260 (= F268)

1glbG Structure of the regulatory complex of escherichia coli iiiglc with glycerol kinase (see paper)
62% identity, 100% coverage: 3:497/497 of query aligns to 1:489/489 of 1glbG

query
sites
1glbG

K

K

Y

Y

V

I

M

V

A

A

L

L

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R
|
R

C

A

I

V

I

V

F

M

N

D

E

H

N

D

G

A

L

N

P

I

V

I

S

S

T

V

S

S

Q

Q

K

R

E

E

F

F

K

E

Q

Q

I

I

Y

Y

P

P

E

K

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

M

E

E

I

I

W

W

A

A

T

T

Q

Q

F

S

G

S

V

T

A

L

T

V

E

E

T

V

L

L

L

A

K

K

S

A

N

D

I

I

K

S

A

S

E

D

D

Q

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

I

V

V

W

W

D

E

R

K

R

E

T

T

G

G

L

K

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

I

C

C

D

E

E

H

L

L

K

K

A

R

K

D

G

G

V

L

D

E

K

D

I

Y

I

I

K

R

E

S

K

N

T

T

G

G

L

L

V

V

L

I

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

S

R

R

R

E

E

R

A

A

N

R

R

R

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

M

T

T

K

Q

G

G

K

R

V

V

H

H

V

V

T

T

D

D

Y

Y

T

T

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

T

L

L

K

D

W

W

D

D

K

D

E

K

L

M

L

L

E

E

L

V

F

L

D

D

I

I

P

P

E

R

S

E

M

M

L

L

P

P

E

E

V

V

K

R

P

R

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Q

A

T

A

N

E

I

S

-

L

-

F

-

G

-

V

-

E

-

I

I

P

P

I

I

A

S

G

G

D

I

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

L

C

C

F

V

K

K

P

E

G

G

M

M

A

A

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F
|
F

L

M

L

L

M

M

N

N

T

T

G

G

D

E

K

K

A

A

V

V

E

K

S

S

K

E

N

N

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

C

G

G

I

P

D

T

G

G

K

E

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

A

I

V

F

F

I

M

G

A

G
|
G

A

A

S

S

I
|
I

Q

Q

W

W

L

L

R

R

D

D

E

E

L

M

R

K

M

L

L

I

K

N

T

D

A
|
A

P

Y

E

D

S

S

E

E

K

Y

Y

F

C

A

D

T

A

K

V

V

E

Q

D

N

T

T

G

N

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

P

Y

Y

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

V

K

N

K

A

E

N

H

H

F

I

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

I

A

A

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

L

K

E

A

A

M

M

E

Q

E

A

D

D

S

S

H

G

I

I

E

R

L

L

K

H

A

A

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

C

V

A

A

N
|
N

N

N

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

S

D

D

I

I

L

L

N

G

V

T

Q

R

V

V

D

E

R

R

P

P

K

E

V

V

I

R

E

E

T

V

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

Q

N

N

R

L

E

D

D

E

V

L

R

Q

K

E

N

K

W

A

A

V

I

I

S

E

K

R

N

E

F

F

M

R

P

P

V

G

M

I

K

E

E

T

D

T

K

E

R

R

E

N

K

Y

L

R

L

Y

E

A

G

G

W

W

H

K

D

K

A

A

V

V

K

K

R

R

S

A

M

M

G

A

W

W

A

E

K

E

Q

H

binding adenosine-5'-diphosphate: R14 (= R16), G256 (= G264), T257 (= T265), G300 (= G308), I303 (= I311), A316 (= A324), G401 (= G409), A402 (= A410), N405 (= N413)
binding glycerol: R80 (= R82), E81 (= E83), W100 (= W102), Y132 (= Y134), D235 (= D243), F260 (= F268)

3ge1A 2.7 angstrom crystal structure of glycerol kinase (glpk) from staphylococcus aureus in complex with adp and glycerol
61% identity, 98% coverage: 1:489/497 of query aligns to 2:491/499 of 3ge1A

query
sites
3ge1A

M

M

A

E

K

K

Y

Y

V

I

M

L

A

S

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

C

A

I

I

I

L

F

F

N

N

E

Q

N

K

G

G

L

E

P

I

V

A

S

G

T

V

S

A

Q

Q

K

R

E

E

F

F

K

K

Q

Q

I

Y

Y

F

P

P

E

Q

G

S

G

G

W

W

V

V

E

E

H

H

D

D

P

A

M

N

E

E

I

I

W

W

A

T

T

S

Q

V

F

L

G

A

V

V

A

M

T

T

E

E

T

V

L

I

L

N

K

E

S

N

N

D

I

V

K

R

A

A

E

D

D

Q

I

I

E

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

V

V

W

W

D

D

R

K

R

H

T

T

G

G

L

R

P

P

V

I

Y

Y

N

H

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

T

T

A

Q

D

S

Y

I

C

C

D

S

E

E

L

L

K

K

A

Q

K

Q

G

G

V

Y

D

E

K

Q

I

T

I

F

K

R

E

D

K

K

T

T

G

G

L

L

V

L

L

L

D

D

A

P

Y

Y

F

F

S

A

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

N

V

V

P

E

T

G

A

A

R

R

R

E

E

K

A

A

N

E

R

N

G

G

N

D

L

L

I

L

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

M

V

W

W

N

K

L

L

T

S

K

G

G

K

K

A

V

A

H

H

V

I

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

L

L

M

F

F

N

N

I

I

H

H

E

D

L

L

K

E

W

W

D

D

K

D

E

E

L

L

L

L

E

E

L

L

F

L

D

T

I

V

P

P

E

K

S

N

M

M

L

L

P

P

E

E

V

V

K

K

P

A

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

K

A

T

A

I

E

D

-

Y

S

H

L

F

F

Y

G

G

V

Q

E

E

I

V

P

P

I

I

A

A

G

G

D

V

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

A

C

C

F

F

K

E

P

R

G

G

M

D

A

V

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

G

F

F

L

M

L

L

M

M

N

N

T

T

G

G

D

D

K

K

A

A

V

V

E

K

S

S

K

E

N

S

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

Y

G

G

I

I

D

D

G

G

K

K

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

S

I

I

F

F

I

V

G

S

G
|
G

A

S

S

A

I
|
I

Q
|
Q

W

W

L

L

R

R

D

D

E

G

L

L

R

R

M

M

L

I

K

N

T

S

A
|
A

P
|
P

E

Q

S

S

E

E

K

S

Y

Y

C

A

D

T

A

R

V

V

E

D

D

S

T

T

G

E

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

V

G

G

L

L

G

G

A

T

P

P

Y

Y

W

W

D

D

Q

S

Y

E

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

T

K

E

K

K

E

E

H

H

F

F

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

L

A

C

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

M

K

E

A

A

M

M

E

S

E

K

D

D

S

S

H

G

I

I

E

D

L

V

K

Q

A

S

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

C

V

A

K

N
|
N

N

N

F

F

L

I

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

V

N

N

V

T

Q

S

V

V

D

E

R

R

P

P

K

E

V

I

I

Q

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

F

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

E

N

S

R

K

E

D

D

D

V

I

R

A

K

K

N

N

W

W

A

K

I

L

S

E

K

E

N

K

F

F

M

D

P

P

V

K

M

M

K

D

E

E

D

G

K

E

R

R

E

E

K

K

L

L

L

Y

E

R

G

G

W

W

H

K

D

K

A

A

V

V

K

E

binding adenosine-5'-diphosphate: G266 (= G264), T267 (= T265), G310 (= G308), I313 (= I311), Q314 (= Q312), A326 (= A324), P327 (= P325), G411 (= G409), A412 (= A410), N415 (= N413)

Q5HGD2 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Staphylococcus aureus (strain COL)
61% identity, 98% coverage: 1:489/497 of query aligns to 1:490/498 of Q5HGD2

query
sites
Q5HGD2

M

M

A

E

K

K

Y

Y

V

I

M

L

A

S

L

I

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R
|
R

C

A

I

I

I

L

F

F

N

N

E

Q

N

K

G

G

L

E

P

I

V

A

S

G

T

V

S

A

Q

Q

K

R

E

E

F

F

K

K

Q

Q

I

Y

Y

F

P

P

E

Q

G

S

G

G

W

W

V

V

E

E

H

H

D

D

P

A

M

N

E

E

I

I

W

W

A

T

T

S

Q

V

F

L

G

A

V

V

A

M

T

T

E

E

T

V

L

I

L

N

K

E

S

N

N

D

I

V

K

R

A

A

E

D

D

Q

I

I

E

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

V

V

W

W

D

D

R

K

R

H

T

T

G

G

L

R

P

P

V

I

Y

Y

N

H

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

T

T

A

Q

D

S

Y

I

C

C

D

S

E

E

L

L

K

K

A

Q

K

Q

G

G

V

Y

D

E

K

Q

I

T

I

F

K

R

E

D

K

K

T

T

G

G

L

L

V

L

L

L

D

D

A

P

Y
|
Y

F

F

S

A

A

G

T

T

K

K

I

V

K

K

W

W

I

I

L

L

D

D

N

N

V

V

P

E

T

G

A

A

R

R

R

E

E

K

A

A

N

E

R

N

G

G

N

D

L

L

I

L

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

M

V

W

W

N

K

L

L

T

S

K

G

G

K

K

A

V

A

H

H

V

I

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

L

L

M

F

F

N

N

I

I

H

H

E

D

L

L

K

E

W

W

D

D

K

D

E

E

L

L

L

L

E

E

L

L

F

L

D

T

I

V

P

P

E

K

S

N

M

M

L

L

P

P

E

E

V

V

K

K

P

A

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

K

A

T

A

I

E

D

-

Y

S

H

L

F

F

Y

G

G

V

Q

E

E

I

V

P

P

I

I

A

A

G

G

D

V

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

A

C

C

F

F

K

E

P

R

G

G

M

D

A

V

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

G

F

F

L

M

L

L

M

M

N

N

T

T

G

G

D

D

K

K

A

A

V

V

E

K

S

S

K

E

N

S

G

G

L

L

L

L

T

T

T

T

I

I

A

A

I

Y

G

G

I

I

D

D

G

G

K

K

V

V

E

N

Y

Y

A

A

L

L

E

E

G

G

S

S

I

I

F

F

I

V

G

S

G
|
G

A

S

S

A

I

I

Q
|
Q

W

W

L

L

R

R

D

D

E

G

L

L

R

R

M

M

L

I

K

N

T

S

A

A

P

P

E

Q

S

S

E

E

K

S

Y

Y

C

A

D

T

A

R

V

V

E

D

D

S

T

T

G

E

G

G

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

V

G

G

L

L

G

G

A

T

P

P

Y

Y

W

W

D

D

Q

S

Y

E

A

A

R

R

G

G

I

A

M

I

I

F

G

G

I

L

T

T

R

R

G

G

T

T

K

E

K

K

E

E

H

H

F

F

V

I

R

R

A

A

T

T

V

L

E

E

S

S

L

L

A

C

Y

Y

Q

Q

T

T

Y

R

D

D

V

V

L

M

K

E

A

A

M

M

E

S

E

K

D

D

S

S

H

G

I

I

E

D

L

V

K

Q

A

S

L

L

K

R

V

V

D

D

G

G

G
|
G

A

A

C

V

A

K

N
|
N

N

N

F

F

L

I

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

V

N

N

V

T

Q

S

V

V

D

E

R

R

P

P

K

E

V

I

I

Q

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

F

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

E

N

S

R

K

E

D

D

D

V

I

R

A

K

K

N

N

W

W

A

K

I

L

S

E

K

E

N

K

F

F

M

D

P

P

V

K

M

M

K

D

E

E

D

G

K

E

R

R

E

E

K

K

L

L

L

Y

E

R

G

G

W

W

H

K

D

K

A

A

V

V

K

E

T12 (= T12) binding ADP
R16 (= R16) binding ADP
R82 (= R82) binding glycerol
E83 (= E83) binding glycerol
Y134 (= Y134) binding glycerol
D244 (= D243) binding glycerol
Q245 (= Q244) binding glycerol
T266 (= T265) binding ADP
G309 (= G308) binding ADP
Q313 (= Q312) binding ADP
G410 (= G409) binding ADP
N414 (= N413) binding ADP

6udeB Crystal structure of glycerol kinase from elizabethkingia anophelis nuhp1 in complex with adp and glycerol
61% identity, 99% coverage: 3:494/497 of query aligns to 3:492/495 of 6udeB

query
sites
6udeB

K

K

Y

L

V

I

M

L

A

A

L

L

D

D

Q

Q

G
|
G

T
|
T

T
|
T

S
|
S

S

S

R
|
R

C

A

I

I

I

L

F

F

N

N

E

K

N

S

G

G

L

E

P

I

V

K

S

F

T

V

S

S

Q

Q

K

K

E

S

F

F

K

E

Q

Q

I

I

Y

F

P

P

E

T

G

P

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

N

E

E

I

I

W

W

A

S

T

S

Q

Q

F

I

G

S

V

V

A

A

T

A

E

E

T

V

L

I

L

A

K

K

S

A

N

G

I

I

K

S

A

G

E

L

D

E

I

V

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

V

V

W

W

D

D

R

R

R

H

T

T

G

S

L

E

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

D

R

R

R

R

T

T

A

S

D

K

Y

Y

C

C

D

D

E

E

L

L

K

K

A

S

K

Q

G

G

V

H

D

T

K

D

I

E

I

I

K

K

E

Q

K

K

T

T

G

G

L

L

V

V

L

L

D

D

A

A

Y

Y

F

F

S

S

A

A

T

T

K

K

I

L

K

K

W

W

I

I

L

L

D

D

N

N

V

V

P

E

T

G

A

A

R

R

R

E

E

K

A

A

N

E

R

A

G

G

N

D

L

L

I

C

F

F

G

G

T

T

V

V

D

D

T

T

W

W

L

L

M

I

W

W

N

K

L

L

T

T

K

R

G

G

K

K

V

M

H

F

V

I

T

T

D

D

Y

V

T

S

N

N

A

A

S

S

R

R

T

T

M

M

L

M

F

F

N

N

I

I

H

R

E

T

L

M

K

D

W

W

D

D

K

D

E

D

L

L

L

L

E

K

L

L

F

F

D

N

I

I

P

P

E

R

S

A

M

I

L

L

P

P

E

E

V

V

K

K

P

Q

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

E

A

T

A

S

E

T

S

T

L

-

F

-

G

S

V

T

E

K

I

I

P

P

I

I

A

A

G

G

D

I

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

M

C

C

F

T

K

K

P

P

G

G

M

M

A

V

K

K

N

N

T

T

Y

Y

G
|
G

T
|
T

G

G

C

C

F

F

L

L

L

L

M

M

N

N

T

T

G

G

D

N

K

E

A

A

V

V

E

Y

S

S

K

K

N

N

G

N

L

L

L

L

T

T

T

T

I

V

A

A

I

W

G

K

I

I

D

N

G

G

K

E

V

V

E

S

Y

Y

A

A

L

L

E

E

G

G

S

S

I

V

F

F

I

V

G

G

G
|
G

A

A

S

A

I
|
I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

R

K

M

I

L

I

K

H

T

D

A

S

P
x
S

E

E

S

V

E

S

K

T

Y

L

C

A

D

E

A

T

V

V

E

E

D

D

T

N

G

G

G

G

V

V

Y

Y

L

F

V

V

P

P

A

A

F

L

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

Q

Y

Y

A

A

R

R

G

G

I

T

M

I

I

I

G

G

I

V

T

T

R

R

G

G

T

T

K

T

K

D

E

G

H

H

F

I

V

A

R

R

A

A

T

T

V

L

E

E

S

G

L

I

A

A

Y

F

Q

Q

T

V

Y

Y

D

D

V

I

L

V

K

K

A

A

M

M

E

E

E

A

D

D

S

A

H

E

I

T

E

Q

L

S

K

T

A

E

L

L

K

R

V

V

D

D

G
|
G

G
|
G

A
|
A

C

S

A

A

N

S

N

N

F

L

L

L

M

M

Q

Q

F

I

Q

Q

S

S

D

D

I

L

L

F

N

G

V

F

Q

K

V

I

D

I

R

R

P

P

K

K

V

T

I

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

E

N

S

R

I

E

D

D

E

V

I

R

Q

K

S

N

Q

W

W

A

I

I

I

S

E

K

K

N

E

F

F

M

T

P

P

V

K

M

E

K

D

E

K

D

T

K

K

R

I

E

D

K

N

L

M

L

V

E

S

G

F

W

W

H

H

D

K

A

A

V

V

K

K

R

R

S

S

M

Q

G

A

W

W

binding adenosine-5'-diphosphate: R16 (= R16), G262 (= G264), T263 (= T265), G306 (= G308), I309 (= I311), S323 (≠ P325), G406 (= G408), G407 (= G409), A408 (= A410)
binding magnesium ion: G11 (= G11), T12 (= T12), T13 (= T13), S14 (= S14)

O34154 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus faecalis (strain ATCC 700802 / V583) (see 2 papers)
59% identity, 98% coverage: 3:488/497 of query aligns to 5:490/501 of O34154

query
sites
O34154

K

K

Y

Y

V

I

M

M

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

C

A

I

I

I

I

F

F

N

D

E

K

N

K

G

G

L

N

P

K

V

I

S

G

T

S

S

S

Q

Q

K

K

E

E

F

F

K

T

Q

Q

I

Y

Y

F

P

P

E

N

G

A

G

G

W

W

V

V

E

E

H

H

D

N

P

A

M

N

E

E

I

I

W

W

A

N

T

S

Q

V

F

Q

G

S

V

V

A

I

T

A

E

G

T

S

L

L

L

I

K

E

S

S

N

G

I

V

K

K

A

P

E

T

D

D

I

I

E

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

V

V

V

W

W

D

D

R

K

R

A

T

T

G

G

L

L

P

P

V

I

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

T

T

A

T

D

P

Y

I

C

A

D

D

E

Q

L

L

K

K

A

E

K

D

G

G

V

Y

D

S

K

E

I

M

I

I

K

H

E

E

K

K

T

T

G

G

L

L

V

I

L

I

D

D

A

A

Y

Y

F

F

S

S

A

A

T

T

K

K

I

V

K

R

W

W

I

I

L

L

D

D

N

H

V

V

P

E

T

G

A

A

R

Q

R

E

E

R

A

A

N

E

R

N

G

G

N

E

L

L

I

M

F

F

G

G

T

T

V

I

D

D

T

T

W

W

L

L

M

V

W

W

N

K

L

L

T

T

K

-

G

G

K

D

V

T

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

H

E

D

L

L

K

D

W

W

D

D

K

Q

E

E

L

I

L

L

E

D

L

L

F

L

D

N

I

I

P

P

E

R

S

V

M

M

L

L

P

P

E

K

V

V

K

V

P

S

S

N

S

S

C

E

V

V

Y

Y

G

G

Q

L

A

T

A

K

E

N

-

Y

S
x
H

L

F

F

Y

G

G

V

S

E

E

I

V

P

P

I

I

A

A

G

G

D

M

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

M

C

A

F

F

K

E

P

P

G

G

M

M

A

V

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

S

F

F

L

I

L

V

M

M

N

N

T

T

G

G

D

E

K

E

A

P

V

Q

E

L

S

S

K

K

N

N

G

N

L

L

L

L

T

T

T

T

I

I

A

G

I

Y

G

G

I

I

D

N

G

G

K

K

V

V

E

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

I

I

F

F

I

V

G

A

G

G

A

S

S

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

R

K

M

M

L

L

K

Q

T

T

A

A

P

A

E

E

S

S

E

E

K

A

Y

V

C

A

D

K

A

A

V

S

E

T

D

G

T

H

G

N

G

E

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

S

Y

Q

A

A

R

R

G

G

I

A

M

V

I

F

G

G

I

L

T

T

R

R

G

G

T

T

K

T

K

R

E

E

H

D

F

F

V

V

R

K

A

A

T

T

V

L

E

Q

S

A

L

V

A

A

Y

Y

Q

Q

T

V

Y

R

D

D

V

I

L

I

K

D

A

T

M

M

E

K

E

E

D

D

S

T

H

G

I

I

E

D

L

I

K

P

A

V

L

L

K

K

V

V

D

D

G

G

G

G

A

A

C

A

A

N

N

N

N

D

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

N

V

T

Q

A

V

V

D

Q

R

R

P

A

K

H

V

N

I

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

F

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

K

N

D

R

L

E

E

D

E

V

I

R

K

K

A

N

F

W

Q

A

E

I

E

S

G

K

Q

N

Q

F

F

M

E

P

P

V

I

M

M

K

A

E

E

D

E

K

E

R

R

E

E

K

D

L

L

L

Y

E

E

G

G

W

W

H

Q

D

Q

A

A

V

V

H231 (≠ S229) modified: Phosphohistidine; by HPr

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

O34153 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Enterococcus casseliflavus (Enterococcus flavescens) (see 3 papers)
58% identity, 98% coverage: 4:488/497 of query aligns to 6:491/506 of O34153

query
sites
O34153

Y

Y

V

V

M

M

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

S

R

R

C

A

I

I

I

I

F

F

N

D

E

R

N

N

G

G

L

K

P

K

V

I

S

G

T

S

S

S

Q

Q

K

K

E

E

F

F

K

P

Q

Q

I

Y

Y

F

P

P

E

K

G

S

G

G

W

W

V

V

E

E

H

H

D

N

P

A

M

N

E

E

I

I

W

W

A

N

T

S

Q

V

F

Q

G

S

V

V

A

I

T

A

E

G

T

A

L

F

L

I

K

E

S

S

N

G

I

I

K

R

A

P

E

E

D

A

I

I

E

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

V

V

W

W

D

D

R

K

R

T

T

T

G

G

L

Q

P

P

V

I

Y

A

N

N

A

A

I

I

V

V

W

W

Q

Q

C

S

R

R

R

Q

T

S

A

S

D

P

Y

I

C

A

D

D

E

Q

L

L

K

K

A

V

K

D

G

G

V

H

D

T

K

E

I

M

I

I

K

H

E

E

K

K

T

T

G

G

L

L

V

V

L

I

D

D

A

A

Y
|
Y

F

F

S

S

A

A

T

T

K

K

I

V

K

R

W

W

I

L

L

L

D

D

N

N

V

I

P

E

T

G

A

A

R

Q

R

E

E

K

A

A

N

D

R

N

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

I

D

D

T

S

W

W

L

L

M

V

W

W

N

K

L

L

T

T

K

D

G

G

K

Q

V

V

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

Y

N

N

I

I

H

H

E

K

L

L

K

E

W

W

D

D

K

Q

E

E

L

I

L

L

E

D

L

L

F

L

D

N

I

I

P

P

E

S

S

S

M

M

L

L

P

P

E

E

V

V

K

K

P

S

S

N

S

S

C

E

V

V

Y

Y

G

G

Q

H

A

T

-

R

A

S

E

Y

S
x
H

L

F

F

Y

G

G

V

S

E

E

I

V

P

P

I

I

A

A

G

G

D

M

A

A

G

G

D
|
D

Q
|
Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

M

C

A

F

F

K

E

P

K

G

G

M

M

A

I

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

A

F

F

L

I

L

V

M

M

N

N

T

T

G

G

D

E

K

E

A

P

V

Q

E

L

S

S

K

D

N

N

G

D

L

L

L

L

T

T

T

T

I

I

A

G

I

Y

G

G

I

I

D

N

G

G

K

K

V

V

E

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

I

I

F

F

I

V

G

A

G

G

A

S

S

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

R

R

M

M

L

I

K

E

T

T

A

S

P

P

E

Q

S

S

E

E

K

E

Y

L

C

A

D

A

A

K

V

A

E

K

D

G

T

D

G

N

G

E

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

S

Y

E

A

A

R

R

G

G

I

A

M

V

I

F

G

G

I

L

T

T

R

R

G

G

T

T

K

T

K

K

E

E

H

D

F

F

V

V

R

R

A

A

T

T

V

L

E

Q

S

A

L

V

A

A

Y

Y

Q

Q

T

S

Y

K

D

D

V

V

L

I

K

D

A

T

M

M

E

K

E

K

D

D

S

S

H

G

I

I

E

D

L

I

K

P

A

L

L

L

K

K

V

V

D

D

G

G

G

G

A

A

C

A

A

K

N

N

N

D

F

L

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

D

V

I

Q

D

V

V

D

Q

R

R

P

A

K

A

V

N

I

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

K

N

D

R

L

E

D

D

E

V

L

R

K

K

S

N

M

W

A

A

E

I

E

S

G

K

Q

N

M

F

F

M

T

P

P

V

E

M

M

K

P

E

A

D

E

K

E

R

R

E

D

K

N

L

L

L

Y

E

E

G

G

W

W

H

K

D

Q

A

A

V

V

R84 (= R82) binding glycerol
E85 (= E83) binding glycerol
Y136 (= Y134) binding glycerol
H232 (≠ S229) modified: Phosphohistidine; by HPr; mutation to A: Loss of phosphorylation, no effect on activity.; mutation to E: Loss of phosphorylation, 2.5-fold reduced activity.; mutation to R: Loss of phosphorylation, 3.4-fold increased activity.
D246 (= D243) binding glycerol
Q247 (= Q244) binding glycerol

3h3nX Glycerol kinase h232r with glycerol (see paper)
58% identity, 98% coverage: 4:488/497 of query aligns to 5:490/501 of 3h3nX

query
sites
3h3nX

Y

Y

V

V

M

M

A

A

L

I

D

D

Q

Q

G

G

T
|
T

T

T

S

S

S

S

R

R

C

A

I

I

I

I

F

F

N

D

E

R

N

N

G

G

L

K

P

K

V

I

S

G

T

S

S

S

Q

Q

K

K

E

E

F

F

K

P

Q

Q

I

Y

Y

F

P

P

E

K

G

S

G

G

W

W

V

V

E

E

H

H

D

N

P

A

M

N

E

E

I

I

W

W

A

N

T

S

Q

V

F

Q

G

S

V

V

A

I

T

A

E

G

T

A

L

F

L

I

K

E

S

S

N

G

I

I

K

R

A

P

E

E

D

A

I

I

E

A

A

G

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

T

I

V

V

V

W

W

D

D

R

K

R

T

T

T

G

G

L

Q

P

P

V

I

Y

A

N

N

A

A

I

I

V

V

W
|
W

Q

Q

C

S

R

R

R

Q

T

S

A

S

D

P

Y

I

C

A

D

D

E

Q

L

L

K

K

A

V

K

D

G

G

V

H

D

T

K

E

I

M

I

I

K

H

E

E

K

K

T

T

G

G

L

L

V

V

L

I

D

D

A

A

Y
|
Y

F

F

S

S

A

A

T

T

K

K

I

V

K

R

W

W

I

L

L

L

D

D

N

N

V

I

P

E

T

G

A

A

R

Q

R

E

E

K

A

A

N

D

R

N

G

G

N

E

L

L

I

L

F

F

G

G

T

T

V

I

D

D

T

S

W

W

L

L

M

V

W

W

N

K

L

L

T

T

K

D

G

G

K

Q

V

V

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

Y

N

N

I

I

H

H

E

K

L

L

K

E

W

W

D

D

K

Q

E

E

L

I

L

L

E

D

L

L

F

L

D

N

I

I

P

P

E

S

S

S

M

M

L

L

P

P

E

E

V

V

K

K

P

S

S

N

S

S

C

E

V

V

Y

Y

G

G

Q

H

A

T

-

R

A

S

E

Y

S

R

L

F

F

Y

G

G

V

S

E

E

I

V

P

P

I

I

A

A

G

G

D

M

A

A

G

G

D
|
D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

M

C

A

F

F

K

E

P

K

G

G

M

M

A

I

K

K

N

N

T

T

Y

Y

G

G

T
|
T

G

G

C

A

F
|
F

L

I

L

V

M

M

N

N

T

T

G

G

D

E

K

E

A

P

V

Q

E

L

S

S

K

D

N

N

G

D

L

L

L

L

T

T

T

T

I

I

A

G

I

Y

G

G

I

I

D

N

G

G

K

K

V

V

E

Y

Y

Y

A

A

L

L

E

E

G

G

S

S

I

I

F

F

I

V

G

A

G

G

A

S

S

A

I

I

Q

Q

W

W

L

L

R

R

D

D

E

G

L

L

R

R

M

M

L

I

K

E

T

T

A

S

P

P

E

Q

S

S

E

E

K

E

Y

L

C

A

D

A

A

K

V

A

E

K

D

G

T

D

G

N

G

E

V

V

Y

Y

L

V

V

V

P

P

A

A

F

F

V

T

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

S

Y

E

A

A

R

R

G

G

I

A

M

V

I

F

G

G

I

L

T

T

R

R

G

G

T

T

K

T

K

K

E

E

H

D

F

F

V

V

R

R

A

A

T

T

V

L

E

Q

S

A

L

V

A

A

Y

Y

Q

Q

T

S

Y

K

D

D

V

V

L

I

K

D

A

T

M

M

E

K

E

K

D

D

S

S

H

G

I

I

E

D

L

I

K

P

A

L

L

L

K

K

V

V

D

D

G

G

G

G

A

A

C

A

A

K

N

N

N

D

F

L

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

D

V

I

Q

D

V

V

D

Q

R

R

P

A

K

A

V

N

I

L

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

G

Y

F

W

W

Q

K

N

D

R

L

E

D

D

E

V

L

R

K

K

S

N

M

W

A

A

E

I

E

S

G

K

Q

N

M

F

F

M

T

P

P

V

E

M

M

K

P

E

A

D

E

K

E

R

R

E

D

K

N

L

L

L

Y

E

E

G

G

W

W

H

K

D

Q

A

A

V

V

binding glycerol: R83 (= R82), E84 (= E83), W103 (= W102), Y135 (= Y134), D245 (= D243), F270 (= F268)
binding phosphate ion: T13 (= T12), T267 (= T265)

6k76A Glycerol kinase form thermococcus kodakarensis, complex structure with substrate.
59% identity, 99% coverage: 5:496/497 of query aligns to 1:482/485 of 6k76A

query
sites
6k76A

V

V

M

L

A

S

L

L

D

D

Q

E

G

G

T
|
T

T

T

S

S

S

A

R

R

C

A

I

I

I

I

F

F

N

D

E

R

N

E

G

S

L

N

P

I

V

H

S

G

T

I

S

G

Q

Q

K

Y

E

E

F

F

K

P

Q

Q

I

H

Y

Y

P

P

E

R

G

P

G

G

W

W

V

V

E

E

H

H

D

N

P

P

M

E

E

E

I

I

W

W

A

D

T

A

Q

Q

F

L

G

R

V

-

A

A

T

I

E

K

T

D

L

A

L

I

K

Q

S

S

N

A

I

R

K

E

A

P

E

N

D

Q

I

I

E

A

A

A

I

I

G

G

I

V

T

T

N

N

Q

Q

R

R

E

E

T

T

T

T

I

L

V

V

W

W

D

D

R

K

R

-

T

D

G

G

L

K

P

P

V

L

Y

Y

N

N

A

A

I

I

V

V

W

W

Q

Q

C

C

R

R

R

R

T

T

A

A

D

E

Y

M

C

V

D

E

E

E

L

I

K

K

A

-

K

R

G

E

V

Y

D

G

K

T

I

M

I

I

K

K

E

E

K

K

T

T

G

G

L

L

V

V

L

P

D

D

A

A

Y

Y

F

F

S

S

A

A

T

S

K

K

I

L

K

K

W

W

I

L

L

L

D

D

N

N

V

V

P

P

T

G

A

L

R

R

R

E

E

K

A

A

N

E

R

K

G

G

N

E

L

V

I

M

F

F

G

G

T

T

V

V

D

D

T

T

W

F

L

L

M

I

W

Y

N

R

L

L

T

T

K

G

G

E

K

-

V

-

H

H

V

V

T

T

D

D

Y

Y

T

S

N

N

A

A

S

S

R

R

T

T

M

M

L

L

F

F

N

N

I

I

H

K

E

K

L

L

K

D

W

W

D

D

K

D

E

E

L

L

L

L

E

E

L

L

F

F

D

D

I

I

P

P

E

E

S

S

M

V

L

L

P

P

E

E

V

V

K

R

P

E

S

S

S

S

C

E

V

V

Y

Y

G

G

Q

Y

A

T

A

K

E

K

S

E

L

L

F

-

G

-

V

A

E

E

I

I

P

P

I

V

A

S

G

G

D

D

A

A

G

G

D

D

Q

Q

Q

Q

A

A

A

A

L

L

F

F

G

G

Q

Q

T

A

C

A

F

F

K

E

P

A

G

G

M

M

A

V

K

K

N

A

T

T

Y

Y

G

G

T
|
T

G

G

C

S

F

F

L

I

L

L

M

V

N

N

T

T

G

-

D

D

K

K

A

M

V

V

E

L

S

Y

K

S

N

D

G

N

L

L

L

L

T

T

T

T

I

I

A

A

I

W

G

G

I

L

D

N

G

G

K

R

V

V

E

S

Y

Y

A

A

L

L

E

E

G

G

S

S

I

I

F

F

I

V

G

T

G
|
G

A

A

S

A

I
x
V

Q
|
Q

W

W

L

L

R

R

D

D

E

G

L

I

R

K

M

I

L

I

K

K

T

H

A

A

P

S

E

E

S

T

E

E

K

E

Y

L

C

A

D

T

A

K

V

L

E

E

D

S

T

N

G

E

G

G

V

V

Y

Y

L

F

V

V

P

P

A

A

F

F

V

V

G

G

L

L

G

G

A

A

P

P

Y

Y

W

W

D

D

Q

Q

Y

F

A

A

R

R

G

G

I

I

M

I

I

I

G

G

I

I

T

T

R

R

G

G

T

T

K

G

K

R

E

E

H

H

F

L

V

A

R

R

A

A

T

T

V

L

E

E

S

A

L

I

A

A

Y

Y

Q

L

T

T

Y

R

D

D

V

V

L

V

K

D

A

E

M

M

E

E

E

K

D

-

S

-

H

L

I

V

E

Q

L

I

K

K

A

E

L

L

K

R

V

V

D

D

G

G

G
|
G

A
|
A

C

T

A

A

N
|
N

N

D

F

F

L

L

M

M

Q

Q

F

F

Q

Q

S

A

D

D

I

I

L

L

N

N

V

R

Q

K

V

V

D

I

R

R

P

P

K

V

V

V

I

K

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

Y

L

L

A

A

G

G

I

L

A

A

V

V

G

D

Y

Y

W

W

Q

A

N

D

R

T

E

R

D

E

V

I

R

A

K

E

N

L

W

W

A

K

I

A

S

E

K

R

N

I

F

F

M

E

P

P

V

K

M

M

K

D

E

E

D

K

K

T

R

R

E

E

K

R

L

L

L

Y

E

K

G

G

W

W

H

K

D

E

A

A

V

V

K

K

R

R

S

A

M

M

G

G

W

W

A

A

K

K

binding phosphoaminophosphonic acid-adenylate ester: T8 (= T12), T254 (= T265), G296 (= G308), V299 (≠ I311), Q300 (= Q312), G395 (= G409), A396 (= A410), N399 (= N413)

O86033 Glycerol kinase; ATP:glycerol 3-phosphotransferase; Glycerokinase; GK; EC 2.7.1.30 from Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
54% identity, 99% coverage: 1:492/497 of query aligns to 1:494/497 of O86033

query
sites
O86033

M

M

A

G

K

G

Y

Y

V

I

M

L

A

A

L

I

D

D

Q

Q

G

G

T

T

T

T

S

S

S

T

R

R

C

A

I

I

I

V

F

F

N

D

E

G

N

N

G

Q

L

K

P

I

V

A

S

G

T

V

S

G

Q

Q

K

K

E

E

F

F

K

K

Q

Q

I

H

Y

F

P

P

E

K

G

S

G

G

W

W

V

V

E

E

H

H

D

D

P

P

M

E

E

E

I

I

W

W

A

Q

T

T

Q

V

F

V

G

S

V

T

A

V

T

K

E

E

T

A

L

I

L

E

K

K

S

S

N

G

I

I

K

T

A

A

E

N

D

D

I

I

E

A

A

A

I

I

G

G

I

I

T

T

N

N

Q

Q

R
|
R

E
|
E

T

T

T

V

I

V

V

V

W

W

D

D

R

R

R

E

T

T

G

G

L

K

P

P

V

I

Y

H

N

N

A

A

I

I

V

V

W

W

Q

Q

C

D

R

R

R

R

T

T

A

A

D

A

Y

F

C

C

D

D

E

K

L

L

K

K

A

K

K

K

G

G

V

L

D

E

K

K

I

T

I

F

K

V

E

K

K

K

T

T

G

G

L

L

V

L

L

L

D

D

A

P

Y
|
Y

F

F

S

S

A

G

T

T

K

K

I

L

K

N

W

W

I

L

L

L

D

S

N

N

V

V

P

K

T

G

A

A

R

Q

R

V

E

R

A

A

N

A

R

K

G

G

N

E

L

L

I

C

F

F

G

G

T

T

V

I

D

D

T

T

W

F

L

L

M

I

W

W

N

R

L

L

T

T

K

G

G

G

K

E

V

C

H

F

V

C

T

T

D

D

Y

A

T

T

N

N

A

A

S

S

R

R

T

T

M

L

L

L

F

Y

N

N

I

I

H

A

E

E

L

N

K

A

W

W

D

D

K

D

E

E

L

L

L

T

E

E

L

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F

L

D

R

I

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P

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E

M

M

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L

P

P

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E

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V

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K

P

D

S

C

S

A

C

A

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Y

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A

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A

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S

L

L

F

F

G

G

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A

E

A

I

I

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P

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V

A

A

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D
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D

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|
Q

Q

Q

A

A

A

A

L

T

F

I

G

G

Q

Q

T

A

C

C

F

F

K

K

P

P

G

G

M

M

A

L

K

K

N

S

T

T

Y

Y

G

G

T

T

G

G

C

C

F

F

L

A

L

L

M

L

N

N

T

T

G

G

D

K

K

D

A

M

V

V

E

R

S

S

K

K

N

N

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L

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T

T

T

T

I

I

A

A

I

Y

G

R

I

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D

D

G

G

K

E

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T

E

T

Y

Y

A

A

L

L

E

E

G

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Y

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R

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D

V

L

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E

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-

W

-

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L

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A

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W

A

A

I

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N

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F

M

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P

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M

M

K

D

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D

A

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L

R82 (= R82) binding glycerol
E83 (= E83) binding glycerol
Y134 (= Y134) binding glycerol
D243 (= D243) binding glycerol
Q244 (= Q244) binding glycerol

5gn6A Crystal structure of glycerol kinase from trypanosoma brucei gambiense complexed with cumarin derivative-17b (see paper)
45% identity, 99% coverage: 3:496/497 of query aligns to 4:513/513 of 5gn6A

query
sites
5gn6A

K

K

Y

Y

V

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M

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G

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F

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C

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-

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V

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A

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A

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D

K

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E

E

A

A

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G

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I

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E

T

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A

W

W

D

D

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C

N

Y

A

A

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W
|
W

Q

N

C

D

R

L

R

R

T

T

A

Y

D

D

Y

I

C

T

D

K

E

K

L

V

K

T

A

A

K

E

-

L

-

G

G

G

V

G

D

D

K

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I

M

I

F

K

A

E

S

K

K

-

I

T

T

G

G

L

L

V

P

L

V

D

S

A

T

Y
|
Y

F

F

S

A

A

A

T

F

K

K

I

M

K

R

W

W

I

M

L

L

D

E

N

N

V

V

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P

T

A

A

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A

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E

A

A

C

N

R

R

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G

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F

F

G

G

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T

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I

D

D

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T

W

W

L

L

M

M

W

Y

N

K

L

L

T

S

K

G

G

G

K

K

V

A

H

F

V

V

T

T

D

D

Y

V

T

T

N

N

A

A

S

S

R
|
R

T

T

M

F

L

L

F

M

N

D

I

L

H

R

E

T

L

R

K

K

W

W

D

S

K

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E

E

L

L

L

C

E

E

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F

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K

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P

P

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M

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E

M

T

L

L

P

P

E

E

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I

K

R

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S

N

S

S

C

E

V

L

Y

F

G

G

Q

Y

A

V

A

E

E

T

S

D

L

E

F

C

G

G

V

V

-

A

-

A

-

A

-

L

-

N

-

E

E

R

I

T

P

P

I

I

A

M

G

G

D

S

A

I

G

G

D
|
D

Q

Q

Q

Q

A

S

A

A

L

L

F

F

G

G

Q

N

T

M

C

C

F

F

K

E

P

K

G

G

M

E

A

A

K

K

N

N

T

T

Y

Y

G

G

T

T

G

G

C

C

F
|
F

L

L

L

L

M

M

N

N

T

V

G

G

D

E

K

E

A

A

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E

F

S

S

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K

N

H

G

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|
L

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A

G

-

F

-

Q

I

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G

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D

G

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E

Y

Y

Y

A

A

L

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|
E

G

G

S
x
A

I

I

F

A

I

C

G

A

G

G

A

A

S

T

I

V

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E

W

W

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N

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A

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D

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T

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G

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A

A

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L

A

A

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P

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Y

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W

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D

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P

Y

S

A

A

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R

G

G

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M

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I

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R

A

A

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A

V

L

E

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S

A

L

I

A

A

Y

L

Q

Q

T

L

Y

N

D

D

V

V

L

V

K

G

A

S

M

M

E

K

E

R

D

D

S

A

H

G

I

L

E

N

L

L

K

S

A

S

L

L

K

R

V

V

D

D

G

G

G

G

A

L

C

S

A

K

N

N

N

G

F

L

L

L

M

M

Q

E

F

I

Q

Q

S

A

D

S

I

L

L

L

N

G

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Q

D

V

I

D

L

R

V

P

P

K

S

V

M

I

H

E

E

T

T

T

T

A

A

L

L

G

G

A

A

A

A

Y

L

L

C

A

A

G

G

I

L

A

A

V

A

G

G

Y

V

W

W

Q

T

N

S

R

L

E

E

D

E

V

V

R

K

K

-

N

-

W

-

A

A

I

V

S

S

-

R

-

R

-

E

-

N

-

S

-

W

K

K

N

T

F

V

M

S

P

P

V

S

M

G

K

S

E

A

D

M

K

E

R

R

E

E

K

A

L

M

L

I

E

A

G

E

W

W

H

R

D

E

A

A

V

L

K

K

R

R

S

T

M

K

G

-

W

W

A

A

K

K

binding 4-butyl-7,8-bis(oxidanyl)chromen-2-one: Y141 (= Y134), R194 (= R187), F281 (= F268), L298 (= L285), E316 (= E301), A318 (≠ S303)
binding glycerol: R86 (= R82), E87 (= E83), W106 (= W102), D256 (= D243)

Query Sequence

>WP_017752631.1 NCBI__GCF_000816635.1:WP_017752631.1
MAKYVMALDQGTTSSRCIIFNENGLPVSTSQKEFKQIYPEGGWVEHDPMEIWATQFGVAT
ETLLKSNIKAEDIEAIGITNQRETTIVWDRRTGLPVYNAIVWQCRRTADYCDELKAKGVD
KIIKEKTGLVLDAYFSATKIKWILDNVPTARREANRGNLIFGTVDTWLMWNLTKGKVHVT
DYTNASRTMLFNIHELKWDKELLELFDIPESMLPEVKPSSCVYGQAAESLFGVEIPIAGD
AGDQQAALFGQTCFKPGMAKNTYGTGCFLLMNTGDKAVESKNGLLTTIAIGIDGKVEYAL
EGSIFIGGASIQWLRDELRMLKTAPESEKYCDAVEDTGGVYLVPAFVGLGAPYWDQYARG
IMIGITRGTKKEHFVRATVESLAYQTYDVLKAMEEDSHIELKALKVDGGACANNFLMQFQ
SDILNVQVDRPKVIETTALGAAYLAGIAVGYWQNREDVRKNWAISKNFMPVMKEDKREKL
LEGWHDAVKRSMGWAKQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory