SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_017867456.1 NCBI__GCF_000349725.1:WP_017867456.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4xckA Vibrio cholerae o395 ribokinase complexed with adp, ribose and cesium ion. (see paper)
45% identity, 99% coverage: 1:301/303 of query aligns to 1:299/306 of 4xckA

query
sites
4xckA

M

M

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K

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active site: A249 (= A250), A250 (= A251), G251 (= G252), D252 (= D253)
binding adenosine-5'-diphosphate: T220 (= T221), G222 (= G223), S223 (= S224), V242 (= V243), T247 (= T248), A250 (= A251), F254 (= F255), H276 (≠ N278), A279 (≠ S281), V283 (= V285)
binding alpha-D-ribofuranose: N11 (= N11), D13 (= D13), G39 (= G39), K40 (= K40), N43 (= N43), A95 (= A95), I107 (= I107), I109 (= I109), E140 (= E140), T248 (= T249), D252 (= D253)

P0A9J6 Ribokinase; RK; EC 2.7.1.15 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 98% coverage: 4:301/303 of query aligns to 7:302/309 of P0A9J6

query
sites
P0A9J6

V

L

T

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V

V

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x
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NAD 14:16 (≠ NLD 11:13) binding substrate
GKGAN 42:46 (= GKGAN 39:43) binding substrate
E143 (= E140) binding substrate
N187 (= N185) binding ATP
TLGSRG 223:228 (≠ TIGSKG 221:226) binding ATP
GD 254:255 (= GD 252:253) binding ATP
D255 (= D253) binding substrate
H279 (≠ N278) binding ATP

1rk2A E. Coli ribokinase complexed with ribose and adp, solved in space group p212121 (see paper)
44% identity, 98% coverage: 4:301/303 of query aligns to 4:299/305 of 1rk2A

query
sites
1rk2A

V

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active site: A249 (= A250), A250 (= A251), G251 (= G252), D252 (= D253)
binding adenosine-5'-diphosphate: T220 (= T221), G222 (= G223), S223 (= S224), A250 (= A251), G251 (= G252), H276 (≠ N278), A279 (≠ S281)
binding tetrafluoroaluminate ion: G213 (= G214), R215 (≠ E216)
binding magnesium ion: D246 (= D247), A282 (≠ T284), R285 (= R287), S291 (= S293)
binding alpha-D-ribofuranose: N11 (= N11), D13 (= D13), G38 (= G38), G39 (= G39), K40 (= K40), N43 (= N43), E140 (= E140), D252 (= D253)

1gqtB Activation of ribokinase by monovalent cations (see paper)
44% identity, 98% coverage: 4:301/303 of query aligns to 6:301/307 of 1gqtB

query
sites
1gqtB

V

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E

D

active site: A251 (= A250), A252 (= A251), G253 (= G252), D254 (= D253)
binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), S225 (= S224), A252 (= A251), G253 (= G252), H278 (≠ N278), A281 (≠ S281)
binding cesium ion: D248 (= D247), I250 (≠ T249), A284 (≠ T284), R287 (= R287), S293 (= S293)
binding alpha-D-ribofuranose: N13 (= N11), D15 (= D13), G41 (= G39), N45 (= N43), E142 (= E140), D254 (= D253)

8cqxA Ribokinase from t.Sp mutant a92g
37% identity, 98% coverage: 4:301/303 of query aligns to 2:295/300 of 8cqxA

query
sites
8cqxA

V

I

T

L

V

V

G

G

S

S

I

L

N

N

L

M

D

D

T

L

N

V

L

L

R

R

V

V

K

K

R

R

M

L

V

P

K

R

P

P

G

G

E

E

T

T

I

V

H

L

A

G

K

E

E

D

H

Y

Y

Q

S

T

A

H

G

P

G

G

K

K

G

G

A

A

N

N

Q

Q

A

A

V

V

A

A

A

I

S

A

R

R

A

L

G

G

A

G

D

K

T

V

T

R

F

M

I

L

G

G

A

R

V

V

G

G

N

E

D

D

A

P

P

F

G

G

K

Q

Q

A

M

L

M

K

D

S

L

G

L

L

K

A

E

Q

E

E

G

G

I

V

D

D

L

V

S

A

G

W

I

V

D

L

T

E

I

T

A

P

N

G

E

P

S

S

T

-

G

G

Q

T

A

G

F

F

I

I

T

L

V

V

D

D

D

P

E

E

G

G

E

Q

N

N

S

Q

I

I

T

A

I

V

Y

A

A

P

G

G

A

A

N

N

F

A

A

R

F

L

G

V

P

P

K

E

E

-

I

-

D

D

Q

L

K

P

E

A

S

T

L

A

L

F

K

Q

D

G

S

V

D

G

F

V

V

V

I

L

A

L

Q

Q

F

L

E

E

T

I

P

P

I

L

D

E

A

T

T

V

I

V

R

R

A

A

F

A

E

A

I

L

A

G

H

R

K

K

N

A

E

G

V

A

K

R

T

I

I

L

L

L

N

N

P

A

A

A

P

P

G

A

E

H

D

-

S

A

I

L

S

P

A

S

D

E

L

I

L

L

K

Q

V

S

T

V

D

D

M

L

I

L

T

L

P

V

N
|
N

E

E

T

V

E

E

A

A

E

A

T

Q

I

L

T

T

G

-

V

-

H

-

V

-

E

-

D

-

E

E

V

A

S

S

A

P

K

P

K

R

A

T

A

P

A

E

K

E

L

A

H

L

D

A

L

L

G

A

V

R

E

Q

V

L

-

R

-

G

-

R

-

A

-

P

-

Q

-

A

-

Q

V

V

I

V

I

L

T
|
T

I

L

G
|
G

S
x
A

K

Q

G
|
G

A

A

F

V

Y

W

D

-

F

-

N

S

G

G

T

T

S

E

E

E

L

S

-

H

V

F

P

P

A

A

F

F

K

P

V

V

H

R

A

A

V

V

D
|
D

T

T

T
|
T

A

A

G

G

D

D

T

A

F
|
F

I

A

G

G

A

A

M

L

S

A

S

L

V

G

L

L

K

-

A

A

N

E

F

G

S

Q

N

N

L

M

K

R

D

A

A

A

I

L

L

R

F

F

G

A

N
|
N

K

A

A

A

S
x
G

S
x
A

L

L

T
x
A

V
x
T

Q

T

R

R

Y

P

G

G

A

A

Q

Q

P

P

S
|
S

I

L

P

P

Y

F

Q

R

K

D

E

E

I

V

E

E

binding adenosine-5'-diphosphate: N179 (= N185), T217 (= T221), G219 (= G223), A220 (≠ S224), G222 (= G226), F250 (= F255), N272 (= N278), G275 (≠ S281), A276 (≠ S282), T279 (≠ V285)
binding magnesium ion: D242 (= D247), T244 (= T249), A278 (≠ T284), S287 (= S293)

6wk0B Crystal structure of human ribokinase in complex with amppcp and ribose
36% identity, 99% coverage: 4:302/303 of query aligns to 5:306/311 of 6wk0B

query
sites
6wk0B

V

V

T

V

V

V

G

G

S

S

I

C

N

M

L

T

D
|
D

T

L

N

V

L

S

R

L

V

T

K

S

R

R

M

L

V

P

K

K

P

T

G

G

E

E

T

T

I

I

H

H

A

G

K

H

E

K

H

F

Y

F

S

I

A

G

G

F

G

G

G
|
G

K
|
K

G

G

A

A

N
|
N

Q

Q

A

C

V

V

A

Q

A

A

S

A

R

R

A

L

G

G

A

A

D

M

T

T

T

S

F

M

I

V

G

C

A

K

V

V

G

G

N

K

D

D

A

S

P

F

G

G

K

N

Q

D

M

Y

M

I

D

E

L

N

L

L

K

K

E

Q

E

N

G

D

I

I

D

S

L

T

S

E

G

F

I

T

D

Y

T

Q

I

T

A

K

N

D

E

A

S

A

T

T

G

G

Q

T

A
|
A

F

S

I

I

T

I

V

V

D

N

E

E

G

G

E

Q

N

N

S

I

I

I

T

V

I

I

Y

V

A

A

G

G

A

A

N

N

F

L

A

L

F

L

G

N

P

T

K

E

E

D

I

L

D

R

Q

A

K

A

E

A

S

N

L

V

L

I

K

S

D

R

S

A

D

K

F

V

V

M

I

V

A

C

Q

Q

F

L

E
|
E

T

I

P

T

I

P

D

A

A

T

T

S

I

L

R

E

A

A

F

L

E

T

I

M

A

A

H

R

K

R

N

S

E

G

V

V

K

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

G

A

E

I

D

A

S

D

I

L

S

D

A

P

D

Q

L

F

L

Y

K

T

V

L

T

S

D

D

M

V

I

F

T

C

P

C

N
|
N

E

E

T

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

V

L

H

T

V

V

E

G

D

S

E

A

V

A

S

D

A

A

K

G

K

E

A

A

A

L

K

V

L

L

H

L

D

K

L

R

G

G

V

C

E

Q

V

V

I

I

T
|
T

I

L

G
|
G

S
x
A

K

E

G
|
G

A

C

F

V

Y

V

D

L

F

S

N

Q

G

T

T

E

S

P

E

E

-

P

-

K

L

H

V

I

P

P

A
x
T

F

E

K

K

V
|
V

H

K

A

A

V

V

D

D

T

T

A

G

A
|
A

G
|
G

D
|
D

T

S

F

F

I

V

G

G

A

A

M

L

S

A

S

F

V

Y

L

L

K

-

A

A

N

Y

F

Y

S

P

N

N

-

L

-

S

L

L

K

E

D

D

A

M

I

L

L

N

F

R

G

S

N
|
N

K

F

A

I

S
x
A

L

V

T

S

V
|
V

Q

Q

R

A

Y

A

G

G

A

T

Q

Q

P

S

S

S

I

Y

P

P

Y

Y

Q

K

K

K

E

D

I

L

E

P

V

L

binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (≠ S224), G227 (= G226), T243 (≠ A240), V246 (= V243), A254 (= A251), G255 (= G252), N282 (= N278), A285 (≠ S281), A286 (≠ S282), V289 (= V285)
binding alpha-D-ribofuranose: D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), A96 (= A95), E141 (= E140), D256 (= D253)

5c41A Crystal structure of human ribokinase in complex with amppcp in p21 spacegroup and with 4 protomers
36% identity, 99% coverage: 4:302/303 of query aligns to 5:306/317 of 5c41A

query
sites
5c41A

V

V

T

V

V

V

G

G

S

S

I

C

N

M

L

T

D

D

T

L

N

V

L

S

R

L

V

T

K

S

R

R

M

L

V

P

K

K

P

T

G

G

E

E

T

T

I

I

H

H

A

G

K

H

E

K

H

F

Y

F

S

I

A

G

G

F

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

S

A

R

R

A

L

G

G

A

A

D

M

T

T

T

S

F

M

I

V

G

C

A

K

V

V

G

G

N

K

D

D

A

S

P

F

G

G

K

N

Q

D

M

Y

M

I

D

E

L

N

L

L

K

K

E

Q

E

N

G

D

I

I

D

S

L

T

S

E

G

F

I

T

D

Y

T

Q

I

T

A

K

N

D

E

A

S

A

T

T

G

G

Q

T

A

A

F

S

I

I

T

I

V

V

D

N

E

E

G

G

E

Q

N

N

S

I

I

I

T

V

I

I

Y

V

A

A

G

G

A

A

N

N

F

L

A

L

F

L

G

N

P

T

K

E

E

D

I

L

D

R

Q

A

K

A

E

A

S

N

L

V

L

I

K

S

D

R

S

A

D

K

F

V

V

M

I

V

A

C

Q

Q

F

L

E

E

T

I

P

T

I

P

D

A

A

T

T

S

I

L

R

E

A

A

F

L

E

T

I

M

A

A

H

R

K

R

N

S

E

G

V

V

K

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

G

A

E

I

D

A

S

D

I

L

S

D

A

P

D

Q

L

F

L

Y

K

T

V

L

T

S

D

D

M

V

I

F

T

C

P

C

N
|
N

E

E

T

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

V

L

H

T

V

V

E

G

D

S

E

A

V

A

S

D

A

A

K

G

K

E

A

A

A

L

K

V

L

L

H

L

D

K

L

R

G

G

V

C

E

Q

V

V

I

I

T
|
T

I

L

G
|
G

S
x
A

K

E

G
|
G

A

C

F

V

Y

V

D

L

F

S

N

Q

G

T

T

E

S

P

E

E

-

P

-

K

L

H

V

I

P

P

A

T

F

E

K

K

V
|
V

H

K

A

A

V

V

D

D

T

T

A
x
G

A
|
A

G
|
G

D
|
D

T

S

F

F

I

V

G

G

A

A

M

L

S

A

S

F

V

Y

L

L

K

-

A

A

N

Y

F

Y

S

P

N

N

-

L

-

S

L

L

K

E

D

D

A

M

I

L

L

N

F

R

G

S

N
|
N

K

F

A

I

S
x
A

L

V

T

S

V

V

Q

Q

R

A

Y

A

G

G

A

T

Q

Q

P

S

S

S

I

Y

P

P

Y

Y

Q

K

K

K

E

D

I

L

E

P

V

L

active site: G253 (≠ A250), A254 (= A251), G255 (= G252), D256 (= D253)
binding phosphomethylphosphonic acid adenylate ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (≠ S224), G227 (= G226), V246 (= V243), G255 (= G252), N282 (= N278), A285 (≠ S281), A286 (≠ S282)

5c3yA Structure of human ribokinase crystallized with amppnp
36% identity, 99% coverage: 4:302/303 of query aligns to 4:305/306 of 5c3yA

query
sites
5c3yA

V

V

T

V

V

V

G

G

S

S

I

C

N

M

L

T

D

D

T

L

N

V

L

S

R

L

V

T

K

S

R

R

M

L

V

P

K

K

P

T

G

G

E

E

T

T

I

I

H

H

A

G

K

H

E

K

H

F

Y

F

S

I

A

G

G

F

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

S

A

R

R

A

L

G

G

A

A

D

M

T

T

T

S

F

M

I

V

G

C

A

K

V

V

G

G

N

K

D

D

A

S

P

F

G

G

K

N

Q

D

M

Y

M

I

D

E

L

N

L

L

K

K

E

Q

E

N

G

D

I

I

D

S

L

T

S

E

G

F

I

T

D

Y

T

Q

I

T

A

K

N

D

E

A

S

A

T

T

G

G

Q

T

A

A

F

S

I

I

T

I

V

V

D

N

E

E

G

G

E

Q

N

N

S

I

I

I

T

V

I

I

Y

V

A

A

G

G

A

A

N

N

F

L

A

L

F

L

G

N

P

T

K

E

E

D

I

L

D

R

Q

A

K

A

E

A

S

N

L

V

L

I

K

S

D

R

S

A

D

K

F

V

V

M

I

V

A

C

Q

Q

F

L

E

E

T

I

P

T

I

P

D

A

A

T

T

S

I

L

R

E

A

A

F

L

E

T

I

M

A

A

H

R

K

R

N

S

E

G

V

V

K

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

G

A

E

I

D

A

S

D

I

L

S

D

A

P

D

Q

L

F

L

Y

K

T

V

L

T

S

D

D

M

V

I

F

T

C

P

C

N

N

E

E

T

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

V

L

H

T

V

V

E

G

D

S

E

A

V

A

S

D

A

A

K

G

K

E

A

A

A

L

K

V

L

L

H

L

D

K

L

R

G

G

V

C

E

Q

V

V

I

I

T
|
T

I

L

G
|
G

S

A

K

E

G

G

A

C

F

V

Y

V

D

L

F

S

N

Q

G

T

T

E

S

P

E

E

L

P

-

K

-

H

V

I

P

P

A

T

F

E

K

K

V
|
V

H

K

A

A

V

V

D

D

T
|
T

T

T

A
x
G

A
|
A

G
|
G

D
|
D

T

S

F

F

I

V

G

G

A

A

M

L

S

A

S

F

V

Y

L

L

K

-

A

A

N

Y

F

Y

S

P

N

N

-

L

-

S

L

L

K

E

D

D

A

M

I

L

L

N

F

R

G

S

N
|
N

K

F

A

I

S
x
A

L

V

T

S

V

V

Q

Q

R

A

Y

A

G

G

A

T

Q

Q

P

S

S

S

I

Y

P

P

Y

Y

Q

K

K

K

E

D

I

L

E

P

V

L

active site: G252 (≠ A250), A253 (= A251), G254 (= G252), D255 (= D253)
binding amp phosphoramidate: T221 (= T221), G223 (= G223), V245 (= V243), T250 (= T248), G254 (= G252), N281 (= N278), A284 (≠ S281), A285 (≠ S282)

2fv7A Crystal structure of human ribokinase
36% identity, 99% coverage: 4:302/303 of query aligns to 4:305/308 of 2fv7A

query
sites
2fv7A

V

V

T

V

V

V

G

G

S

S

I

C

N

M

L

T

D

D

T

L

N

V

L

S

R

L

V

T

K

S

R

R

M

L

V

P

K

K

P

T

G

G

E

E

T

T

I

I

H

H

A

G

K

H

E

K

H

F

Y

F

S

I

A

G

G

F

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

S

A

R

R

A

L

G

G

A

A

D

M

T

T

T

S

F

M

I

V

G

C

A

K

V

V

G

G

N

K

D

D

A

S

P

F

G

G

K

N

Q

D

M

Y

M

I

D

E

L

N

L

L

K

K

E

Q

E

N

G

D

I

I

D

S

L

T

S

E

G

F

I

T

D

Y

T

Q

I

T

A

K

N

D

E

A

S

A

T

T

G

G

Q

T

A

A

F

S

I

I

T

I

V

V

D

N

E

E

G

G

E

Q

N

N

S

I

I

I

T

V

I

I

Y

V

A

A

G

G

A

A

N

N

F

L

A

L

F

L

G

N

P

T

K

E

E

D

I

L

D

R

Q

A

K

A

E

A

S

N

L

V

L

I

K

S

D

R

S

A

D

K

F

V

V

M

I

V

A

C

Q

Q

F

L

E

E

T

I

P

T

I

P

D

A

A

T

T

S

I

L

R

E

A

A

F

L

E

T

I

M

A

A

H

R

K

R

N

S

E

G

V

V

K

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

G

A

E

I

D

A

S

D

I

L

S

D

A

P

D

Q

L

F

L

Y

K

T

V

L

T

S

D

D

M

V

I

F

T

C

P

C

N
|
N

E

E

T

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

V

L

H

T

V

V

E

G

D

S

E

A

V

A

S

D

A

A

K

G

K

E

A

A

A

L

K

V

L

L

H

L

D

K

L

R

G

G

V

C

E

Q

V

V

I

I

T
|
T

I

L

G
|
G

S

A

K

E

G
|
G

A

C

F

V

Y

V

D

L

F

S

N

Q

G

T

T

E

S

P

E

E

-

P

-

K

L

H

V

I

P

P

A
x
T

F

E

K

K

V
|
V

H

K

A

A

V

V

D

D

T

T

A
x
G

A
|
A

G
|
G

D
|
D

T

S

F

F

I

V

G

G

A

A

M

L

S

A

S

F

V

Y

L

L

K

-

A

A

N

Y

F

Y

S

P

N

N

-

L

-

S

L

L

K

E

D

D

A

M

I

L

L

N

F

R

G

S

N
|
N

K

F

A

I

S
x
A

L

V

T

S

V
|
V

Q

Q

R

A

Y

A

G

G

A

T

Q

Q

P

S

S

S

I

Y

P

P

Y

Y

Q

K

K

K

E

D

I

L

E

P

V

L

active site: G252 (≠ A250), A253 (= A251), G254 (= G252), D255 (= D253)
binding adenosine-5'-diphosphate: N185 (= N185), T221 (= T221), G223 (= G223), G226 (= G226), T242 (≠ A240), V245 (= V243), A253 (= A251), G254 (= G252), N281 (= N278), A284 (≠ S281), A285 (≠ S282), V288 (= V285)

6wjzA Crystal structure of human ribokinase in complex with ampcp
36% identity, 99% coverage: 4:302/303 of query aligns to 5:306/315 of 6wjzA

query
sites
6wjzA

V

V

T

V

V

V

G

G

S

S

I

C

N

M

L

T

D

D

T

L

N

V

L

S

R

L

V

T

K

S

R

R

M

L

V

P

K

K

P

T

G

G

E

E

T

T

I

I

H

H

A

G

K

H

E

K

H

F

Y

F

S

I

A

G

G

F

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

S

A

R

R

A

L

G

G

A

A

D

M

T

T

T

S

F

M

I

V

G

C

A

K

V

V

G

G

N

K

D

D

A

S

P

F

G

G

K

N

Q

D

M

Y

M

I

D

E

L

N

L

L

K

K

E

Q

E

N

G

D

I

I

D

S

L

T

S

E

G

F

I

T

D

Y

T

Q

I

T

A

K

N

D

E

A

S

A

T

T

G

G

Q

T

A

A

F

S

I

I

T

I

V

V

D

N

E

E

G

G

E

Q

N

N

S

I

I

I

T

V

I

I

Y

V

A

A

G

G

A

A

N

N

F

L

A

L

F

L

G

N

P

T

K

E

E

D

I

L

D

R

Q

A

K

A

E

A

S

N

L

V

L

I

K

S

D

R

S

A

D

K

F

V

V

M

I

V

A

C

Q

Q

F

L

E

E

T

I

P

T

I

P

D

A

A

T

T

S

I

L

R

E

A

A

F

L

E

T

I

M

A

A

H

R

K

R

N

S

E

G

V

V

K

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

G

A

E

I

D

A

S

D

I

L

S

D

A

P

D

Q

L

F

L

Y

K

T

V

L

T

S

D

D

M

V

I

F

T

C

P

C

N
|
N

E

E

T

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

V

L

H

T

V

V

E

G

D

S

E

A

V

A

S

D

A

A

K

G

K

E

A

A

A

L

K

V

L

L

H

L

D

K

L

R

G

G

V

C

E

Q

V

V

I

I

T
|
T

I

L

G
|
G

S
x
A

K

E

G
|
G

A

C

F

V

Y

V

D

L

F

S

N

Q

G

T

T

E

S

P

E

E

-

P

-

K

L

H

V

I

P

P

A
x
T

F

E

K

K

V
|
V

H

K

A

A

V

V

D

D

T

T

A

G

A
|
A

G
|
G

D

D

T

S

F

F

I

V

G

G

A

A

M

L

S

A

S

F

V

Y

L

L

K

-

A

A

N

Y

F

Y

S

P

N

N

-

L

-

S

L

L

K

E

D

D

A

M

I

L

L

N

F

R

G

S

N
|
N

K

F

A

I

S
x
A

L

V

T

S

V
|
V

Q

Q

R

A

Y

A

G

G

A

T

Q

Q

P

S

S

S

I

Y

P

P

Y

Y

Q

K

K

K

E

D

I

L

E

P

V

L

binding phosphomethylphosphonic acid adenosyl ester: N186 (= N185), T222 (= T221), G224 (= G223), A225 (≠ S224), G227 (= G226), T243 (≠ A240), V246 (= V243), A254 (= A251), G255 (= G252), N282 (= N278), A285 (≠ S281), A286 (≠ S282), V289 (= V285)

Q9H477 Ribokinase; RK; EC 2.7.1.15 from Homo sapiens (Human)
35% identity, 97% coverage: 8:302/303 of query aligns to 22:319/322 of Q9H477

query
sites
Q9H477

G

G

S

S

I

C

N

M

L

T

D

D

T

L

N

V

L

S

R

L

V

T

K

S

R

R

M

L

V

P

K

K

P

T

G

G

E

E

T

T

I

I

H

H

A

G

K

H

E

K

H

F

Y

F

S

I

A

G

G

F

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

S

A

R

R

A

L

G

G

A

A

D

M

T

T

T

S

F

M

I

V

G

C

A

K

V

V

G

G

N

K

D

D

A

S

P

F

G

G

K

N

Q

D

M

Y

M

I

D

E

L

N

L

L

K

K

E

Q

E

N

G

D

I

I

D

S

L

T

S

E

G

F

I

T

D

Y

T

Q

I

T

A

K

N

D

E

A

S

A

T

T

G

G

Q

T

A

A

F

S

I

I

T

I

V

V

D

N

E

E

G

G

E

Q

N

N

S

I

I

I

T

V

I

I

Y

V

A

A

G

G

A

A

N

N

F

L

A

L

F

L

G

N

P

T

K

E

E

D

I

L

D

R

Q

A

K

A

E

A

S

N

L

V

L

I

K

S

D

R

S

A

D

K

F

V

V

M

I

V

A

C

Q

Q

F

L

E

E

T

I

P

T

I

P

D

A

A

T

T

S

I

L

R

E

A

A

F

L

E

T

I

M

A

A

H

R

K

R

N

S

E

G

V

V

K

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

G

A

E

I

D

A

S

D

I

L

S

D

A

P

D

Q

L

F

L

Y

K

T

V

L

T

S

D

D

M

V

I

F

T

C

P

C

N
|
N

E

E

T

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

V

L

H

T

V

V

E

G

D

S

E

A

V

A

S

D

A

A

K

G

K

E

A

A

A

L

K

V

L

L

H

L

D

K

L

R

G

G

V

C

E

Q

V

V

I

I

T
|
T

I
x
L

G
|
G

S
x
A

K
x
E

G
|
G

A

C

F

V

Y

V

D

L

F

S

N

Q

G

T

T

E

S

P

E

E

L

P

-

K

-

H

V

I

P

P

A
x
T

F

E

K

K

V

V

H

K

A

A

V

V

D

D

T

T

A

G

A

A

G
|
G

D
|
D

T

S

F

F

I

V

G

G

A

A

M

L

S

A

S

F

V

Y

L

L

K

-

A

A

N

Y

F

Y

S

P

N

N

-

L

-

S

L

L

K

E

D

D

A

M

I

L

L

N

F

R

G

S

N
|
N

K

F

A

I

S

A

L

V

T

S

V

V

Q

Q

R

A

Y

A

G

G

A

T

Q

Q

P

S

S

S

I

Y

P

P

Y

Y

Q

K

K

K

E

D

I

L

E

P

V

L

N199 (= N185) binding ATP
TLGAEG 235:240 (≠ TIGSKG 221:226) binding ATP
T256 (≠ A240) binding ATP
GD 268:269 (= GD 252:253) binding ATP
N295 (= N278) binding ATP

5byfA Crystal structure of human ribokinase in complex with amp
35% identity, 97% coverage: 8:302/303 of query aligns to 10:307/313 of 5byfA

query
sites
5byfA

G

G

S

S

I

C

N

M

L

T

D

D

T

L

N

V

L

S

R

L

V

T

K

S

R

R

M

L

V

P

K

K

P

T

G

G

E

E

T

T

I

I

H

H

A

G

K

H

E

K

H

F

Y

F

S

I

A

G

G

F

G

G

K

K

G

G

A

A

N

N

Q

Q

A

C

V

V

A

Q

A

A

S

A

R

R

A

L

G

G

A

A

D

M

T

T

T

S

F

M

I

V

G

C

A

K

V

V

G

G

N

K

D

D

A

S

P

F

G

G

K

N

Q

D

M

Y

M

I

D

E

L

N

L

L

K

K

E

Q

E

N

G

D

I

I

D

S

L

T

S

E

G

F

I

T

D

Y

T

Q

I

T

A

K

N

D

E

A

S

A

T

T

G

G

Q

T

A

A

F

S

I

I

T

I

V

V

D

N

E

E

G

G

E

Q

N

N

S

I

I

I

T

V

I

I

Y

V

A

A

G

G

A

A

N

N

F

L

A

L

F

L

G

N

P

T

K

E

E

D

I

L

D

R

Q

A

K

A

E

A

S

N

L

V

L

I

K

S

D

R

S

A

D

K

F

V

V

M

I

V

A

C

Q

Q

F

L

E

E

T

I

P

T

I

P

D

A

A

T

T

S

I

L

R

E

A

A

F

L

E

T

I

M

A

A

H

R

K

R

N

S

E

G

V

V

K

K

T

T

I

L

L

F

N

N

P

P

A

A

P

P

G

A

E

I

D

A

S

D

I

L

S

D

A

P

D

Q

L

F

L

Y

K

T

V

L

T

S

D

D

M

V

I

F

T

C

P

C

N

N

E

E

T

S

E

E

A

A

E

E

T

I

I

L

T

T

G

G

V

L

H

T

V

V

E

G

D

S

E

A

V

A

S

D

A

A

K

G

K

E

A

A

A

L

K

V

L

L

H

L

D

K

L

R

G

G

V

C

E

Q

V

V

I

I

T
|
T

I

L

G
|
G

S
x
A

K

E

G
|
G

A

C

F

V

Y

V

D

L

F

S

N

Q

G

T

T

E

S

P

E

E

-

P

-

K

L

H

V

I

P

P

A

T

F

E

K

K

V

V

H

K

A

A

V

V

D

D

T

T

A
x
G

A
|
A

G
|
G

D
|
D

T

S

F

F

I

V

G

G

A

A

M

L

S

A

S

F

V

Y

L

L

K

-

A

A

N

Y

F

Y

S

P

N

N

-

L

-

S

L

L

K

E

D

D

A

M

I

L

L

N

F

R

G

S

N
|
N

K

F

A

I

S
x
A

S

A

L

V

T

S

V

V

Q

Q

R

A

Y

A

G

G

A

T

Q

Q

P

S

S

S

I

Y

P

P

Y

Y

Q

K

K

K

E

D

I

L

E

P

V

L

active site: G254 (≠ A250), A255 (= A251), G256 (= G252), D257 (= D253)
binding adenosine monophosphate: T223 (= T221), G225 (= G223), A226 (≠ S224), G228 (= G226), N283 (= N278), A286 (≠ S281)

6ilsB Structure of arabidopsis thaliana ribokinase complexed with ribose and atp (see paper)
36% identity, 98% coverage: 4:300/303 of query aligns to 5:305/313 of 6ilsB

query
sites
6ilsB

V

L

T

V

V

V

G

G

S

S

I

A

N
|
N

L

A

D
|
D

T

I

N

Y

L

V

R

E

V

I

K

E

R

R

M

L

V

P

K

K

P

E

G

G

E

E

T

T

I

I

H

S

A

A

K

K

E

T

H

G

Y

Q

S

T

A

L

G

A

G

G

G
|
G

K
|
K

G

G

A

A

N
|
N

Q

Q

A

A

V

A

A

C

A

G

S

A

R

K

A

L

G

M

A

Y

D

P

T

T

T

Y

F

F

I

V

G

G

A

R

V

L

G

G

N

E

D

D

A

A

P

H

G

G

K

K

Q

L

M

I

M

A

D

E

L

A

L

L

K

G

E

D

-

G

-

C

G

G

I

V

D

H

L

L

S

D

G

Y

I

V

D

R

T

S

I

V

A

N

N

N

E

E

S

P

T

T

G

G

Q

H

A

A

F

V

I

V

T

M

V

L

D

Q

D

S

E

D

G

G

E

Q

N

N

S

S

I

I

T

I

I

I

Y

V

A

G

G

G

A

A

N

N

F

M

A

K

F

A

G

W

P

P

K

E

E

I

I

M

-

S

D

D

Q

D

K

D

E

L

S

E

L

I

L

V

K

R

D

N

S

A

D

G

F

I

V

V

I

L

A

L

Q

Q

F

R

E
|
E

T

I

P

P

I

D

D

S

A

I

T

N

I

I

R

Q

A

V

F

A

E

K

I

A

A

V

H

K

K

K

N

A

E

G

V

V

K

P

T

V

I

I

L

L

N

D

P

V

A

G

P

G

G

M

E

D

D

T

S

P

I

I

S

P

A

N

D

E

L

L

K

D

V

S

T

I

D

D

M

I

I

L

T

S

P

P

N
|
N

E

E

T

T

E

E

A

L

E

S

T

R

I

L

T

T

G

G

V

M

H

P

V

T

E

E

D

T

E

F

V

E

S

Q

A

I

K

S

K

Q

A

A

A

V

A

A

K

K

L

C

H

H

D

K

L

L

G

G

V

V

E

K

V

Q

V

V

I

L

I

V

T
x
K

I

L

G
|
G

S

S

K

K

G

G

A

S

F

A

Y

L

D

F

F

I

N

Q

G

G

-

E

-

K

-

P

-

I

T

Q

S

Q

E

S

L

I

V
x
I

P

P

A
|
A

F

A

K

Q

V

V

H

-

A

-

V

V

D

D

T

T

A

G

A
|
A

G
|
G

D
|
D

T

T

F
|
F

I

T

G

A

A

A

M

F

S

A

S

V

V

A

L

M

K

V

A

E

N

G

F

K

S

S

N

H

L

-

K

E

D

E

A

C

I

L

L

R

F

F

G

A

N

A

K

A

A

A

S
x
A

S
|
S

L

L

T

C

V

V

Q

Q

R

V

Y

K

G

G

A

A

Q

I

P

P

S

S

I

M

P

P

Y

D

Q

R

K

K

E

S

I

V

binding adenosine-5'-triphosphate: N189 (= N185), K225 (≠ T221), G227 (= G223), I246 (≠ V238), A248 (= A240), A257 (= A251), G258 (= G252), F261 (= F255), A286 (≠ S281), S287 (= S282)
binding alpha-D-ribofuranose: N12 (= N11), D14 (= D13), G40 (= G39), K41 (= K40), N44 (= N43), E144 (= E140), D259 (= D253)

A1A6H3 Ribokinase; AtRBSK; RK; EC 2.7.1.15 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 98% coverage: 4:300/303 of query aligns to 71:371/379 of A1A6H3

query
sites
A1A6H3

V
x
L

T
x
V

V
|
V

G

G

S

S

I

A

N

N

L

A

D

D

T

I

N

Y

L

V

R

E

V

I

K

E

R

R

M

L

V

P

K

K

P

E

G

G

E

E

T

T

I

I

H

S

A

A

K

K

E

T

H

G

Y

Q

S

T

A

L

G

A

G

G

K

K

G

G

A

A

N

N

Q

Q

A

A

V

A

A

C

A

G

S

A

R

K

A

L

G

M

A

Y

D

P

T

T

T

Y

F

F

I

V

G

G

A

R

V

L

G

G

N

E

D

D

A

A

P

H

G

G

K

K

Q

L

M

I

M

A

D

E

L

A

L

L

K

G

E

D

-

G

-

C

G

G

I

V

D

H

L

L

S

D

G

Y

I

V

D

R

T

S

I

V

A

N

N

N

E

E

S

P

T

T

G

G

Q

H

A

A

F

V

I

V

T

M

V

L

D

Q

D

S

E

D

G

G

E

Q

N

N

S

S

I

I

T

I

I

I

Y

V

A

G

G

G

A

A

N

N

F

M

A

K

F

A

G

W

P

P

K

E

E

I

I

M

-

S

D

D

Q

D

K

D

E

L

S

E

L

I

L

V

K

R

D

N

S

A

D

G

F

I

V

V

I

L

A

L

Q

Q

F

R

E

E

T

I

P

P

I

D

D

S

A

I

T

N

I

I

R

Q

A

V

F

A

E

K

I

A

A

V

H

K

K

K

N

A

E

G

V

V

K

P

T

V

I

I

L

L

N

D

P

V

A

G

P

G

G

M

E

D

D

T

S

P

I

I

S

P

A

N

D

E

L

L

K

D

V

S

T

I

D

D

M

I

I

L

T

S

P

P

N

N

E

E

T

T

E

E

A

L

E

S

T

R

I

L

T

T

G

G

V

M

H

P

V

T

E

E

D

T

E

F

V

E

S

Q

A

I

K

S

K

Q

A

A

A

V

A

A

K

K

L

C

H

H

D

K

L

L

G

G

V

V

E

K

V

Q

V

V

I

L

I

V

T

K

I

L

G

G

S

S

K

K

G

G

A

S

F

A

Y

L

D

F

F

I

N

Q

G

G

-

E

-

K

-

P

-

I

T

Q

S

Q

E

S

L

I

V

I

P

P

A

A

F

A

K

Q

V

V

H

-

A

-

V

V

D

D

T

T

A

G

A

A

G

G

D

D

T

T

F

F

I

T

G

A

A

A

M

F

S

A

S

V

V

A

L

M

K

V

A

E

N

G

F

K

S

S

N

H

L

-

K

E

D

E

A

C

I

L

L

R

F

F

G

A

N

A

K

A

A

A

S

A

S

S

L

L

T

C

V

V

Q

Q

R

V

Y

K

G

G

A

A

Q

I

P

P

S

S

I

M

P

P

Y

D

Q

R

K

K

E

S

I

V

Sites not aligning to the query:

1:74 mutation Missing: Loss of ribokinase activity.

6a8cA Ribokinase from leishmania donovani with adp (see paper)
36% identity, 98% coverage: 4:300/303 of query aligns to 16:322/327 of 6a8cA

query
sites
6a8cA

V

I

T

L

V

V

G

G

S

S

I

C

N

F

L

L

D
|
D

T

Y

N

V

L

G

R

Y

V

V

K

D

R

H

M

M

V

P

K

Q

P

V

G

G

E

E

T

T

I

M

H

H

A
x
S

K

V

E
x
S

H

F

Y

H

S

K

A

G

G

F

G
|
G

K

K

G

G

A

A

N
|
N

Q

Q

A

A

V

V

A

A

S

G

R

R

A

L

G

G

A

A

D

K

T

V

T

A

F

M

I

V

G

S

A

M

V

V

G

G

N

T

D

D

A

G

P

D

G

G

K

S

Q

D

M

Y

M

I

D

K

L

E

L

L

K

E

E

R

E

N

G

G

I

V

D

D

L

T

S

A

G

Y

I

M

D

F

T

R

I

T

A

G

N

K

E

S

S

S

T

T

G

G

Q

L

A

A

F

M

I

I

T

L

V

V

D

D

D

T

E

K

G

S

E

S

N

N

S

N

-

E

I

I

T

V

I

I

Y

C

A

P

G

N

A

A

N

T