SitesBLAST
Comparing WP_017894758.1 NCBI__GCF_000816635.1:WP_017894758.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
39% identity, 99% coverage: 4:481/485 of query aligns to 2:483/485 of 4griB
- active site: S9 (= S11), K253 (= K254)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E43), Y194 (= Y195), R212 (= R213), W216 (≠ Y217)
- binding zinc ion: C105 (= C107), C107 (= C109), Y128 (= Y130), C132 (= C134)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
38% identity, 100% coverage: 1:485/485 of query aligns to 1:474/488 of 8vc5A
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
37% identity, 98% coverage: 4:477/485 of query aligns to 2:467/468 of 2cv2A
- active site: K246 (= K254)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (= S11), G17 (= G19), I21 (≠ T23), E41 (= E43), Y187 (= Y195), R205 (= R213), A206 (≠ G214), E208 (= E216), W209 (≠ Y217), L235 (≠ P243), L236 (≠ I244)
- binding : S9 (= S11), T43 (= T45), D44 (= D46), R47 (= R49), V145 (≠ I153), R163 (≠ Y171), Y168 (≠ V176), E172 (= E180), V177 (≠ I185), K180 (= K188), S181 (= S189), Y187 (= Y195), E207 (≠ S215), E208 (= E216), W209 (≠ Y217), V211 (≠ S219), R237 (≠ M245), K241 (≠ H249), L272 (= L280), M273 (≠ L281), G274 (= G282), E282 (= E290), S299 (≠ N307), P303 (≠ A311), V304 (≠ I312), K309 (= K317), W312 (= W320), R319 (≠ K327), P357 (≠ T361), R358 (= R362), R417 (≠ M426), Q432 (≠ W441), R435 (= R444), L442 (≠ F452), E443 (≠ S453), T444 (≠ A454), G446 (= G456), L447 (≠ A457), F448 (≠ Y458)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
37% identity, 98% coverage: 4:477/485 of query aligns to 2:467/468 of 2cv1A
- active site: K246 (= K254)
- binding adenosine-5'-triphosphate: P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ N20), I21 (≠ T23), R47 (= R49), A206 (≠ G214), W209 (≠ Y217), L235 (≠ P243), L236 (≠ I244)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (= E43), Y187 (= Y195), R205 (= R213), W209 (≠ Y217)
- binding : S9 (= S11), E41 (= E43), T43 (= T45), D44 (= D46), R47 (= R49), V145 (≠ I153), R163 (≠ Y171), V166 (≠ I174), E172 (= E180), V177 (≠ I185), K180 (= K188), S181 (= S189), Y187 (= Y195), E207 (≠ S215), E208 (= E216), W209 (≠ Y217), V211 (≠ S219), R237 (≠ M245), K241 (≠ H249), K243 (= K251), M273 (≠ L281), G274 (= G282), S276 (≠ N284), E282 (= E290), S299 (≠ N307), P303 (≠ A311), V304 (≠ I312), K309 (= K317), W312 (= W320), R319 (≠ K327), P357 (≠ T361), R358 (= R362), R417 (≠ M426), L427 (≠ N436), Q432 (≠ W441), R435 (= R444), L442 (≠ F452), E443 (≠ S453), T444 (≠ A454), G446 (= G456), L447 (≠ A457), F448 (≠ Y458)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
37% identity, 98% coverage: 4:477/485 of query aligns to 2:467/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
37% identity, 98% coverage: 4:477/485 of query aligns to 2:467/468 of 1n78A
- active site: K246 (= K254)
- binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ N20), I21 (≠ T23), E41 (= E43), Y187 (= Y195), N191 (= N199), R205 (= R213), A206 (≠ G214), E208 (= E216), W209 (≠ Y217), L235 (≠ P243), L236 (≠ I244)
- binding : S9 (= S11), T43 (= T45), D44 (= D46), R47 (= R49), V145 (≠ I153), R163 (≠ Y171), V166 (≠ I174), Y168 (≠ V176), E172 (= E180), V177 (≠ I185), K180 (= K188), S181 (= S189), Y187 (= Y195), E207 (≠ S215), E208 (= E216), W209 (≠ Y217), L210 (= L218), V211 (≠ S219), R237 (≠ M245), K241 (≠ H249), M273 (≠ L281), G274 (= G282), E282 (= E290), R297 (≠ N305), P303 (≠ A311), V304 (≠ I312), K309 (= K317), W312 (= W320), R319 (≠ K327), P357 (≠ T361), R358 (= R362), R417 (≠ M426), L427 (≠ N436), Q432 (≠ W441), R435 (= R444), L442 (≠ F452), E443 (≠ S453), T444 (≠ A454), G446 (= G456), L447 (≠ A457), F448 (≠ Y458)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
37% identity, 98% coverage: 4:477/485 of query aligns to 2:467/468 of 1j09A
- active site: K246 (= K254)
- binding adenosine-5'-triphosphate: H15 (= H17), E208 (= E216), L235 (≠ P243), L236 (≠ I244), K243 (= K251), I244 (≠ L252), S245 (= S253), K246 (= K254), R247 (= R255)
- binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E43), Y187 (= Y195), N191 (= N199), R205 (= R213), W209 (≠ Y217)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
37% identity, 98% coverage: 4:477/485 of query aligns to 2:467/468 of P27000
- R358 (= R362) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
37% identity, 98% coverage: 4:477/485 of query aligns to 2:467/468 of 1g59A
- binding : D44 (= D46), R45 (≠ Q47), A46 (≠ E48), R47 (= R49), P109 (≠ K111), V145 (≠ I153), R163 (≠ Y171), V166 (≠ I174), E172 (= E180), V177 (≠ I185), K180 (= K188), S181 (= S189), D182 (= D190), E207 (≠ S215), E208 (= E216), R237 (≠ M245), K241 (≠ H249), T242 (≠ H250), K243 (= K251), M273 (≠ L281), G274 (= G282), E282 (= E290), S299 (≠ N307), L300 (≠ K308), P303 (≠ A311), V304 (≠ I312), K309 (= K317), W312 (= W320), R319 (≠ K327), P357 (≠ T361), R358 (= R362), R417 (≠ M426), K426 (= K435), L427 (≠ N436), Q432 (≠ W441), R435 (= R444), L442 (≠ F452), E443 (≠ S453), T444 (≠ A454), P445 (≠ G455), G446 (= G456), L447 (≠ A457), F448 (≠ Y458)
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
40% identity, 99% coverage: 4:481/485 of query aligns to 103:563/564 of 3al0C
- active site: S110 (= S11), K335 (= K254)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (= T23), E142 (= E43), Y276 (= Y195), R294 (= R213), G295 (= G214), D297 (≠ E216), H298 (≠ Y217), L324 (≠ P243), I325 (= I244), L333 (= L252)
- binding : T144 (= T45), D145 (= D46), R148 (= R49), Y208 (≠ C109), P213 (≠ N120), K252 (≠ Y171), M255 (≠ I174), I266 (= I185), K269 (= K188), S270 (= S189), Y276 (= Y195), D297 (≠ E216), H298 (≠ Y217), L299 (= L218), S300 (= S219), N301 (≠ S220), K304 (= K223), R330 (≠ H249), P332 (≠ K251), G363 (= G282), W364 (= W283), R365 (≠ N284), E370 (= E290), S387 (≠ N307), K389 (≠ S309), V391 (≠ A311), I392 (= I312), K397 (= K317), W400 (= W320), R407 (≠ K327), E446 (≠ T361), K447 (≠ R362), Q453 (≠ E368), I457 (≠ L372), R509 (≠ M426), K520 (≠ G437), Q524 (≠ W441), R527 (= R444), V535 (≠ S453), T536 (≠ A454), G538 (= G456), L539 (≠ A457)
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
38% identity, 99% coverage: 3:481/485 of query aligns to 2:502/502 of 6brlA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
36% identity, 98% coverage: 3:478/485 of query aligns to 2:462/471 of P04805
- C98 (= C107) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C109) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C134) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (= H136) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ S138) mutation to Q: No change in activity or in zinc content.
- H131 (≠ E140) mutation to Q: No change in activity or in zinc content.
- H132 (≠ E141) mutation to Q: No change in activity or in zinc content.
- C138 (≠ Y152) mutation to S: No change in activity or in zinc content.
- S239 (= S253) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
36% identity, 98% coverage: 3:478/485 of query aligns to 2:462/468 of 8i9iA
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
34% identity, 92% coverage: 4:449/485 of query aligns to 2:449/484 of 2cfoA
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
34% identity, 92% coverage: 4:449/485 of query aligns to 3:450/485 of Q8DLI5
- R6 (= R7) binding L-glutamate
- Y192 (= Y195) binding L-glutamate
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
35% identity, 79% coverage: 4:386/485 of query aligns to 3:362/380 of 4g6zA
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
34% identity, 62% coverage: 2:300/485 of query aligns to 14:283/308 of P27305
- E55 (= E43) binding L-glutamate
- Y182 (= Y195) binding L-glutamate
- R200 (= R213) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
33% identity, 62% coverage: 2:300/485 of query aligns to 2:269/290 of 4a91A
- active site: S11 (= S11), K229 (= K254)
- binding glutamic acid: R7 (= R7), A9 (= A9), S11 (= S11), E43 (= E43), Y170 (= Y195), R188 (= R213), L192 (≠ Y217)
- binding zinc ion: C99 (= C107), C101 (= C109), Y113 (= Y130), C117 (= C134)
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
32% identity, 49% coverage: 3:240/485 of query aligns to 10:228/455 of 3aiiA
Sites not aligning to the query:
P46655 Glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; (c)ERS; GluRS; P85; EC 6.1.1.17 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 48% coverage: 1:231/485 of query aligns to 199:418/708 of P46655
Sites not aligning to the query:
- 148 R→A: Abolishes interaction with ARC1.
Query Sequence
>WP_017894758.1 NCBI__GCF_000816635.1:WP_017894758.1
MTKVRTRFAPSPTGYMHVGNLRTALYAYLIAKHEGGDFILRIEDTDQERFVEGALDIIYD
TLKITGLKHDEGPDIGGPVGPYIQSKRTNIYIEYAKQLIEKGEAYYCFCSKERLDELHKN
AESAKQTYKYDKHCLHLSKEEIQENLNSGVPYIIRQNNPETGTTVFHDEIYGDISVDNSE
LDDMILIKSDGFPTYNFANVVDDHLMGITHVVRGSEYLSSSPKYNRLYEAFGWQVPIYVH
CPPIMKDTHHKLSKRNGDASFEDLIKKGYLKEAIINYIALLGWNPGGENEIYSLNELVKA
FNYKNINKSPAIFDDVKLKWMNGEYIKKMNTDKFTEMAIPYYKKIITKDLNFKKISELVQ
TRIEVLPEICDLIDFFQDLPEYSADIYVHKKMKTTFENSLLTLEKILPVFEAIDDWNIKN
IEDACMNLVKELSVKNGIVLWPIRTALSGKKFSAGGAYEIADIIGKEESLKRLSIGIDKL
KSAIK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory