SitesBLAST
Comparing WP_018123677.1 NCBI__GCF_000375485.1:WP_018123677.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5nofA Anthranilate phosphoribosyltransferase from thermococcus kodakaraensis (see paper)
39% identity, 96% coverage: 6:328/336 of query aligns to 2:312/325 of 5nofA
1kgzB Crystal structure analysis of the anthranilate phosphoribosyltransferase from erwinia carotovora (current name, pectobacterium carotovorum) (see paper)
37% identity, 96% coverage: 7:327/336 of query aligns to 5:318/330 of 1kgzB
- binding manganese (ii) ion: S92 (= S98), D222 (= D231), E223 (= E232), E223 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V79 (≠ C85), G82 (= G88), G83 (= G89), N90 (= N96), I91 (≠ C97), S92 (= S98), T93 (= T99), K108 (= K114), S118 (= S126)
2gvqD Anthranilate phosphoribosyl-transferase (trpd) from s. Solfataricus in complex with anthranilate (see paper)
35% identity, 96% coverage: 7:329/336 of query aligns to 6:328/345 of 2gvqD
P50384 Anthranilate phosphoribosyltransferase; EC 2.4.2.18 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see 2 papers)
35% identity, 96% coverage: 7:329/336 of query aligns to 6:328/345 of P50384
- T87 (≠ S94) binding 5-phospho-alpha-D-ribose 1-diphosphate
- K106 (= K114) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency dedreases only 10-fold.
- H107 (= H115) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency. 300-fold decrease of the affinity for anthranilate, whereas catalytic efficiency remains nearly unchanged; when associated with A-178.
- S118 (= S126) binding 5-phospho-alpha-D-ribose 1-diphosphate
- H154 (= H162) mutation to A: Limited effect on either affinity for anthranilate and catalytic efficiency.
- R164 (= R172) mutation to A: Strong decrease of the affinity for anthranilate, although only a moderate 7-fold decrease in catalytic efficiency.
- D223 (= D231) mutation to N: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
- E224 (= E232) mutation to Q: Affinity for phosphoribosylpyrophosphate is similar to that of the wild-type enzyme and catalytic efficiency is unchanged.
1zxyA Anthranilate phosphoribosyltransferase from sulfolobus solfataricus in complex with prpp and magnesium (see paper)
35% identity, 96% coverage: 7:329/336 of query aligns to 6:328/344 of 1zxyA
- binding magnesium ion: D223 (= D231), E224 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ C85), G79 (= G86), D83 (= D90), T87 (≠ S94), N89 (= N96), S91 (= S98), T92 (= T99), K106 (= K114), S118 (= S126), D223 (= D231), E224 (= E232)
1gxbA Anthranilate phosphoribosyltransferase in complex with pyrophosphate and magnesium (see paper)
34% identity, 96% coverage: 7:329/336 of query aligns to 6:324/339 of 1gxbA
3gbrA Anthranilate phosphoribosyl-transferase (trpd) double mutant d83g f149s from s. Solfataricus (see paper)
35% identity, 96% coverage: 7:329/336 of query aligns to 6:325/341 of 3gbrA
- binding manganese (ii) ion: S91 (= S98), D220 (= D231), E221 (= E232), E221 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: A78 (≠ C85), G79 (= G86), T80 (= T87), G81 (= G88), N89 (= N96), V90 (≠ C97), S91 (= S98), T92 (= T99), K106 (= K114), G114 (= G125)
5c7sA Prpp complexed with two mn2+ in the active site of mycobacterium tuberculosis anthranilate phosphoribosyltransferase (anprt; trpd)
34% identity, 89% coverage: 6:305/336 of query aligns to 6:301/344 of 5c7sA
- active site: V83 (≠ C85)
- binding manganese (ii) ion: G84 (= G86), S96 (= S98), D228 (= D231), E229 (= E232), E229 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (≠ C85), G84 (= G86), G86 (= G88), G87 (= G89), N94 (= N96), S96 (= S98), T97 (= T99), K112 (= K114), N115 (= N117), S120 (= S122)
4owmA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-fluoroanthranilate, prpp and magnesium (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/346 of 4owmA
- active site: V82 (≠ C85)
- binding 2-azanyl-3-fluoranyl-benzoic acid: P156 (= P159), H159 (= H162), Y162 (≠ F165), R163 (≠ K166), A166 (≠ M169), R170 (≠ Q173)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G86), G86 (= G89), N93 (= N96), S95 (= S98), T96 (= T99), K111 (= K114), A117 (≠ L120), S118 (= S121), S119 (= S122), G122 (= G125)
4gkmA Bianthranilate-like analogue bound in the outer site of anthranilate phosphoribosyltransferase (anprt; trpd) (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/346 of 4gkmA
- active site: V82 (≠ C85)
- binding 2-[(2-carboxyphenyl)amino]-5-methylbenzoic acid: N114 (= N117), A155 (= A158), P156 (= P159), Y162 (≠ F165), R163 (≠ K166), A166 (≠ M169), R169 (= R172), R170 (≠ Q173)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G86 (= G89), N93 (= N96), S95 (= S98), T96 (= T99), K111 (= K114), A117 (≠ L120), S118 (= S121), S119 (= S122), G122 (= G125)
3r6cA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs179) (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/346 of 3r6cA
- active site: V82 (≠ C85)
- binding 8-methoxyphenanthro[3,4-d][1,3]dioxole-5,6-dicarboxylic acid: M62 (≠ G63), N114 (= N117), A155 (= A158), P156 (= P159), H159 (= H162), Y162 (≠ F165), A166 (≠ M169), R169 (= R172), G182 (= G185), T185 (≠ L188), R239 (≠ F243), A241 (≠ H245), D246 (≠ E250)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G86), G85 (= G88), G86 (= G89), N93 (= N96), S95 (= S98), T96 (= T99), K111 (= K114), N114 (= N117), R115 (= R118), A116 (= A119), A117 (≠ L120), S118 (= S121), S119 (= S122), G122 (= G125), G123 (≠ S126)
Sites not aligning to the query:
3qsaA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor tamu-a7)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/346 of 3qsaA
- active site: V82 (≠ C85)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G86), G86 (= G89), N93 (= N96), S95 (= S98), T96 (= T99), K111 (= K114), A117 (≠ L120), S118 (= S121), S119 (= S122), G122 (= G125)
- binding 4,4,4-trifluoro-1-(4-methoxyphenyl)butane-1,3-dione: M62 (≠ G63), N114 (= N117), A155 (= A158), P156 (= P159), H159 (= H162), Y162 (≠ F165), R163 (≠ K166), A166 (≠ M169), G182 (= G185)
3qs8A Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs174) (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/346 of 3qs8A
- active site: V82 (≠ C85)
- binding 2-benzylbenzoic acid: M62 (≠ G63), G83 (= G86), N114 (= N117), N114 (= N117), A155 (= A158), A155 (= A158), P156 (= P159), Y162 (≠ F165), Y162 (≠ F165), A166 (≠ M169), R169 (= R172), R169 (= R172), R170 (≠ Q173), L181 (≠ M184), G182 (= G185)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G86), G85 (= G88), G86 (= G89), N93 (= N96), S95 (= S98), T96 (= T99), K111 (= K114), N114 (= N117), A116 (= A119), A117 (≠ L120), S118 (= S121), S119 (= S122), G122 (= G125), E228 (= E232)
3qqsA Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs172) (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/346 of 3qqsA
- active site: V82 (≠ C85)
- binding 2,2'-iminodibenzoic acid: P156 (= P159), H159 (= H162), Y162 (≠ F165), R163 (≠ K166), A166 (≠ M169), R169 (= R172), R170 (≠ Q173)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (≠ C85), G85 (= G88), G86 (= G89), N93 (= N96), S95 (= S98), T96 (= T99), K111 (= K114), R115 (= R118), A117 (≠ L120), S118 (= S121), S119 (= S122), G122 (= G125)
1zvwA The crystal structure of trpd (rv2192c) from mycobacterium tuberculosis in complex with prpp and magnesium (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/346 of 1zvwA
- active site: V82 (≠ C85)
- binding benzamidine: T46 (≠ R47), M47 (≠ A48), K48 (= K49), P50 (≠ E51), D201 (≠ E204)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G83 (= G86), N93 (= N96), S95 (= S98), T96 (= T99), A117 (≠ L120), S118 (= S121), S119 (= S122)
3r88B Anthranilate phosphoribosyltransferase (trpd) from mycobacterium tuberculosis (complex with inhibitor acs145) (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 5:300/344 of 3r88B
- active site: V82 (≠ C85)
- binding 2-amino-4,5-dimethoxybenzoic acid: N114 (= N117), A155 (= A158), P156 (= P159), H159 (= H162), Y162 (≠ F165), R163 (≠ K166), A166 (≠ M169)
- binding magnesium ion: S95 (= S98), D227 (= D231), E228 (= E232), E228 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V82 (≠ C85), G83 (= G86), G85 (= G88), G86 (= G89), N93 (= N96), S95 (= S98), T96 (= T99), K111 (= K114), N114 (= N117), A117 (≠ L120), S118 (= S121), S119 (= S122)
4giuA Bianthranilate-like analogue bound in inner site of anthranilate phosphoribosyltransferase (anprt; trpd). (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 6:301/346 of 4giuA
- active site: V83 (≠ C85)
- binding 2-[(2-carboxy-5-methylphenyl)amino]-3-methylbenzoic acid: G114 (= G116), N115 (= N117), A156 (= A158), H160 (= H162), Y163 (≠ F165), R170 (= R172), G183 (= G185)
- binding magnesium ion: S96 (= S98), D228 (= D231), E229 (= E232), E229 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G86), G86 (= G88), G87 (= G89), N94 (= N96), S96 (= S98), T97 (= T99), K112 (= K114), N115 (= N117), A117 (= A119), A118 (≠ L120), S119 (= S121), S120 (= S122), G123 (= G125)
4n5vA Alternative substrates of mycobacterium tuberculosis anthranilate phosphoribosyl transferase (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 7:302/347 of 4n5vA
- active site: V84 (≠ C85)
- binding 2-amino-4-fluorobenzoic acid: V84 (≠ C85), G85 (= G86), H114 (= H115), G115 (= G116), N116 (= N117), A157 (= A158), A157 (= A158), P158 (= P159), H161 (= H162), Y164 (≠ F165), R165 (≠ K166), A168 (≠ M169), R171 (= R172), G184 (= G185)
- binding magnesium ion: S97 (= S98), D229 (= D231), E230 (= E232), E230 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G85 (= G86), G87 (= G88), G88 (= G89), N95 (= N96), S97 (= S98), T98 (= T99), K113 (= K114), A119 (≠ L120), S120 (= S121), S121 (= S122), G124 (= G125)
4owuA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 5-methylanthranilate, prpp and magnesium (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 6:301/347 of 4owuA
- active site: V83 (≠ C85)
- binding 2-azanyl-5-methyl-benzoic acid: N115 (= N117), P157 (= P159), Y163 (≠ F165), R164 (≠ K166), A167 (≠ M169)
- binding magnesium ion: S96 (= S98), D228 (= D231), E229 (= E232), E229 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: G84 (= G86), G86 (= G88), G87 (= G89), N94 (= N96), S96 (= S98), T97 (= T99), K112 (= K114), A118 (≠ L120), S120 (= S122), G123 (= G125)
4owqA Anthranilate phosphoribosyl transferase from mycobacterium tuberculosis in complex with 3-methylanthranilate, prpp and magnesium (see paper)
34% identity, 89% coverage: 6:305/336 of query aligns to 6:301/347 of 4owqA
- active site: V83 (≠ C85)
- binding 2-azanyl-3-methyl-benzoic acid: P157 (= P159), H160 (= H162), Y163 (≠ F165), A167 (≠ M169), R171 (≠ Q173)
- binding magnesium ion: S96 (= S98), D228 (= D231), E229 (= E232), E229 (= E232)
- binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: V83 (≠ C85), G84 (= G86), G86 (= G88), G87 (= G89), N94 (= N96), S96 (= S98), T97 (= T99), K112 (= K114), A117 (= A119), A118 (≠ L120), S120 (= S122), G123 (= G125)
Query Sequence
>WP_018123677.1 NCBI__GCF_000375485.1:WP_018123677.1
MTTPANILDTLAKGESLTDEQASAMFDRLMNGDLHPAQAGAFLMGLRAKGEDSTDIAAGV
RAGLDRALKIPGYDGPNGKPVIDTCGTGGDGSMSFNCSTATALFLADMGHCVAKHGNRAL
SSSCGSADALEDIGVPIENTPEEAAKQLDAANFTFLFAPAYHPAFKHVMPIRQALGIRTV
FNFMGPLLNPARPSHQLMGVGDPERLQIMGESLLLTGVDRAFVISGTGGFDEVTTFGPAR
GYFIHDGIMEKTTINPDRMGFKAHSPDDVRVQDRAQAVAVLREILDGNGPRAMMDMVAVN
LAACLNLLGEGTMRECADIARDKVNEGLSGGIPNAG
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory