SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_018123679.1 NCBI__GCF_000375485.1:WP_018123679.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P28820 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Bacillus subtilis (strain 168) (see paper)
40% identity, 89% coverage: 34:455/474 of query aligns to 33:453/470 of P28820

query
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P28820

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A283 (= A282) mutation to I: Complete loss of aminodeoxychorismate synthase activity.; mutation to K: Absence of covalent intermediate.; mutation to V: Complete loss of aminodeoxychorismate synthase activity.

7pi1DDD Aminodeoxychorismate synthase component 1
39% identity, 91% coverage: 34:462/474 of query aligns to 31:453/459 of 7pi1DDD

query
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7pi1DDD

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binding magnesium ion: G428 (= G437), E438 (= E447)
binding tryptophan: L33 (= L36), E34 (= E37), S35 (= S38), G39 (= G46), Y41 (= Y48), P242 (= P246), Y243 (≠ F247), M244 (= M248), Q406 (≠ D415), N408 (≠ G417)

A0QX93 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
39% identity, 94% coverage: 15:459/474 of query aligns to 50:512/524 of A0QX93

query
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A0QX93

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T

E

R

R

R

G

G

P

L

Y

Y

G

G

C

V

I

V

G

G

F

Y

L

L

G

D

L

F

D

A

S

G

D

N

S

A

V

-

S

-

L

-

D

D

T

F

G

A

I

I

T

A

I

I

R

R

S

T

L

A

W

L

V

M

R

R

N

N

G

G

Q

T

C

A

H

Y

W

V

Q

Q

A

A

G

G

A

G

G

G

I

V

V

V

H

A

D

D

S

S

V

N

P

G

E

P

K

Y

E

E

W

Y

E

T

E

E

C

A

R

A

N

N

K

K

A

A

R

R

V

A

L

V

M

L

K355 (≠ D299) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5cwaA Structure of anthranilate synthase component i (trpe) from mycobacterium tuberculosis with inhibitor bound (see paper)
39% identity, 94% coverage: 15:460/474 of query aligns to 30:492/505 of 5cwaA

query
sites
5cwaA

A

A

D

D

V

S

Q

E

T

T

P

P

I

L

S

S

L

A

Y

Y

L

R

G

K

L

L

V

A

G

A

D

N

A

R

P

P

G

G

-

T

I

F

L

L

E

E

S

S

A

A

E

E

V

N

D

G

G

R

R

S

L

W

G

S

R

R

Y

W

T

S

L

F

I

I

A

G

W

A

D

G

F

A

R

P

L

T

S

A

L

L

R

T

P

V

E

R

N

E

G

G

K

-

L

-

A

-

V

-

E

Q

A

A

T

V

D

W

E

L

R

G

L

A

K

V

P

P

L

K

E

D

K

A

F

P

S

T

G

G

M

G

N

D

F

P

L

L

D

R

G

A

L

L

R

Q

R

V

V

T

L

L

R

E

A

L

V

L

A

A

V

-

E

-

Q

-

E

-

T

T

D

A

D

D

G

P

P

G

L

L

P

P

A

P

L

L

T

S

R

G

G

G

L

M

Y

V

G

G

Y

F

L

F

G

A

Y

Y

G

D

I

M

A

V

G

R

M

R

L

L

E

E

-

R

-

L

P

P

K

E

L

R

A

A

-

V

-

D

-

L

-

C

-

L

-

P

-

D

-

M

-

L

-

A

-

T

E

D

S

V

V

A

K

A

P

V

E

D

D

H

A

H

E

E

A

G

C

T

L

I

N

T

L

L

P

I

G

A

Q

N

M

A

V

V

L

N

F

W

D

N

H

G

L

T

R

D

H

E

S

R

C

V

C

D

Y

W

L

A

S

Y

L

D

D

D

K

A

G

V

S

A

V

R

P

L

R

D

P

V

V

M

A

T

A

A

E

A

W

L

G

G

T

Q

D

P

L

L

A

P

A

S

P

T

E

V

A

A

S

T

-

F

-

S

-

R

-

P

E

E

P

P

Q

R

M

H

Y

R

P

A

E

Q

R

R

-

T

-

V

D

E

Y

Y

L

G

R

A

A

I

V

V

E

E

K

Y

T

L

K

V

E

D

L

Q

I

I

A

A

D

A

G

G

E

E

C

A

I

F

Q
|
Q

A

V

V

V

L

P

S

S

T

Q

R

R

F

F

T

E

V

M

P

D

L

T

P

D

D

V

D

D

P

P

F

I

R

D

I

V

Y

Y

R

R

R

I

L

L

R

R

Q

V

A

T

N

N

P

P

S

S

P

P

F

Y

M

M

F

Y

Y

L

M

L

K

Q

L

V

P

P

G

N

C

S

D

D

G

G

-

A

-

V

-

D

-

F

T

S

L

I

L

V

G

G

S

S

P

P

E
|
E

M

A

M

L

V

V

R

T

C

V

E

H

E

E

G

G

R

W

L

A

E

T

V

T

R

H

P

P

I

I

A
|
A

G

G

T

T

R

R

P

W

R

R

G

G

G

R

N

T

E

D

S

D

E

E

D

D

L

V

A

L

L

L

A

E

D

K

E

E

L

L

A

A

D

D

P

D

K

K

E

E

R

R

A

A

E
|
E

H

H

V

L

M

M

L

L

V

V

D

D

L

L

G

G

R

R

N

N

D

D

L

L

G

G

R

R

I

V

S

C

N

T

P

P

G

G

T

T

V

V

E

R

V

V

K

E

R

D

F

Y

M

S

N

H

V

I

E

E

R

R

F

Y

S

S

H

H

V

V

M

M

H
|
H

L

L

T

V

S

S

Y

T

V

V

E

T

G

G

E

K

L

L

Q

G

D

E

G

G

L

R

D

T

A

A

V

L

D

D

V

A

L

V

R

T

A

A

T

C

F

F

P

P

A

A

G

G

T
|
T

L

L

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

E

I

L

I

I

S

E

E

E

M

V

E

E

P

K

Q

T

E

R

R

R

G

G

P

L

Y
|
Y

G

G

C

V

I

V

G

G

F

Y

L

L

G

D

L

F

D

A

S

G

D

N

S

A

V

-

S

-

L

-

D

D

T

F

G

A

I

I

T

A

I
|
I

R
|
R

S

T

L

A

W

L

V

M

R

R

N

N

G

G

Q

T

C

A

H

Y

W

V

Q

Q

A
|
A

G
|
G

A

G

G
|
G

I

V

V

V

H

A

D

D

S

S

V

N

P

G

E

S

K

Y

E

E

W

Y

E

N

E
|
E

C

A

R

R

N

N

K
|
K

A

A

R

R

V

A

L

V

M

L

E

N

active site: Q248 (= Q217), E301 (= E266), A317 (= A282), E345 (= E310), H382 (= H347), T409 (= T374), Y433 (= Y398), R453 (= R421), G469 (= G437), E482 (= E450), K486 (= K454)
binding 3-{[(1Z)-1-carboxyprop-1-en-1-yl]oxy}-2-hydroxybenzoic acid: Y433 (= Y398), I452 (= I420), A466 (= A434), G467 (= G435), K486 (= K454)

7bvdA Anthranilate synthase component i (trpe)[mycolicibacterium smegmatis]
38% identity, 94% coverage: 15:459/474 of query aligns to 30:487/499 of 7bvdA

query
sites
7bvdA

A

A

D

D

V

S

Q

E

T

T

P

P

I

L

S

S

L

A

Y

Y

L

R

G

K

L

L

V

A

G

A

D

N

A

R

P

P

G

G

-

T

I

F

L

L

E

E

S

S

A

A

E

E

V

-

D

N

G

R

R

S

L

W

G

S

R

R

Y

W

T

S

L

F

I

I

A

G

W

A

D

G

F

A

R

P

L

S

S

A

L

L

R

T

P

V

E

R

N

D

G

N

K

A

L

A

A

A

V

W

E

L

A

G

T

T

D

A

E

P

R

E

L

G

K

A

P

P

L

-

E

-

K

-

F

-

S
|
S

G
|
G

M

G

N

D

F

P

L

L

D

D

G
x
A

L

L

R

R

R

A

V

T

L

L

R

D

A

L

V

L

A

K

V

T

E

E

Q

A

E

M

T

A

D

G

D

-

G

-

P

-

L

L

P

P

A

P

L

L

T

S

R

S

G

G

L

L

Y

V

G

G

Y

F

L

F

G

A

Y

Y

G

D

I

M

A

V

G

R

M

R

L

L

E

E

-

R

-

L

P

P

K

E

L

L

A

A

-

V

-

D

-

L

-

G

-

L

-

P

-

D

-

M

-

L

-

A

-

T

E

D

S

I

V

A

K

A

P

V

E

D

D

H

A

H

E

E

A

G

C

T

L

I

N

T

L

L

P

I

G

A

Q

N

M

A

V

V

L

N

F

W

D

N

H

G

L

T

R

D

H

E

S

R

C

V

C

D

Y

W

L

A

S

Y

L

D

D

D

K

A

G

V

S

A

V

R

P

L

R

D

P

V

V

M

A

T

A

K

E

A

W

L

G

G

T

Q

D

P

L

L

A

T

A

S

P

A

E

V

A

A

S

T

E

F

P

S

Q

R

M

P

Y

A

P

P

E

D

R

H

-

R

-

A

-

Q

-

R

-

T

-

M

D

E

Y

Y

L

T

R

E

A

I

V

V

E

D

K

K

T

L

K

V

E

G

L

D

I

I

A

E

D

A

G

G

E

E

C

A

I

F

Q
|
Q

A

V

V

V

L

P

S

S

T

Q

R

R

F

F

T

E

V

M

P

D

L

T

P

A

D

A

D

D

P

P

F

L

R

D

I

V

Y

Y

R

R

R

I

L

L

R

R

Q

V

A

T

N

N

P

P

S

S

P

P

F

Y

M

M

F

Y

Y

L

M

L

K

N

L

I

P

P

G

D

C

A

D

D

G

G

-

G

-

L

-

D

-

F

T

S

L

I

L

V

G

G

S

S

P

P

E
|
E

M

A

M

L

V

V

R

T

C

V

E

K

E

D

G

G

R

R

L

A

E

T

V

T

R

H

P

P

I

I

A
|
A

G

G

T

T

R

R

P

W

R

R

G

-

N

-

E

-

S

E

E

E

D

D

L

V

A

L

L

L

A

E

D

K

E

E

L

L

A

A

D

D

P

E

K

K

E

E

R

R

A

A

E
|
E

H

H

V

L

M

M

L

L

V

V

D

D

L

L

G

G

R

R

N

N

D

D

L

L

G

G

R

R

I

V

S

C

N

R

P

P

G

G

T

T

V

V

E

R

V

V

K

D

R

D

F

Y

M

S

N

H

V

I

E

E

R

R

F

Y

S

S

H

H

V

V

M

M

H
|
H

L

L

T

V

S

S

Y

T

V

V

E

T

G

G

E

E

L

L

Q

A

D

E

G

D

L

K

D

T

A

A

V

L

D

D

V

A

L

V

R

T

A

A

T

C

F

F

P

P

A

A

G

G

T
|
T

L

L

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

E

I

L

I

I

S

E

E

E

M

V

E

E

P

K

Q

T

E

R

R

R

G

G

P

L

Y
|
Y

G

G

C

V

I

V

G

G

F

Y

L

L

G

D

L

F

D

A

S

G

D

N

S

A

V

-

S

-

L

-

D

D

T

F

G

A

I

I

T

A

I

I

R
|
R

S

T

L

A

W

L

V

M

R

R

N

N

G

G

Q

T

C

A

H

Y

W

V

Q

Q

A

A

G

G

A

G

G
|
G

I

V

V

V

H

A

D

D

S

S

V

N

P

G

E

P

K

Y

E

E

W

Y

E

T

E
|
E

C

A

R

A

N

N

K
|
K

A

A

R

R

V

A

L

V

M

L

active site: Q248 (= Q217), E301 (= E266), A317 (= A282), E341 (= E310), H378 (= H347), T405 (= T374), Y429 (= Y398), R449 (= R421), G465 (= G437), E478 (= E450), K482 (= K454)
binding pyruvic acid: S93 (= S82), G94 (= G83), A100 (≠ G89)

Q94GF1 Anthranilate synthase alpha subunit 1, chloroplastic; OsASA1; EC 4.1.3.27 from Oryza sativa subsp. japonica (Rice) (see paper)
36% identity, 95% coverage: 15:463/474 of query aligns to 72:568/577 of Q94GF1

query
sites
Q94GF1

A

S

D

D

V

H

Q

L

T

T

P

P

I

V

S

L

L

A

Y

Y

L

R

G

C

L

L

V

V

G

R

D

E

-

D

-

R

-

E

A

A

P

P

G

S

I

F

L

L

L

F

E

E

S

S

A

V

E

E

V

Q

D

G

G

S

-

E

-

G

-

T

R

N

L

V

G

G

R

R

Y

Y

T

S

L

V

I

V

A

G

W

A

D

Q

F

P

R

A

L

M

S

E

L

I

R

V

P

A

E

K

N

A

G

N

K

H

L

V

A

T

V

V

E

-

A

-

T

M

D

D

E

H

R

K

L

M

K

K

P

S

L

R

E

R

K

E

F

-

S

-

G

-

M

-

N

Q

F

F

L

A

D

P

G

D

L

P

R

M

R

K

V

I

L

P

R

R

A

S

V

I

A

-

V

M

E

E

Q

Q

-

W

-

N

-

P

E

Q

T

I

D

V

D

E

G

G

P

L

L

P

P

D

A

A

L

F

T

C

R

G

G

G

L

W

Y

V

G

G

Y

F

L

F

G

S

Y

Y

G

D

I

T

A

V

G

R

M

Y

L

V

E

E

-

T

P

K

K

K

L

L

A

P

E

F

S

S

V

N

K

A

P

P

E

E

D

D

-

D

-

R

-

N

-

L

A

P

E

D

A

I

C

H

L

L

N

G

L

L

P

Y

G

N

Q

D

M

I

V

V

L

V

F

F

D

D

H

H

L

V

R

E

H

K

S

K

C

T

-

H

-

V

-

I

-

H

-

W

-

V

-

R

-

V

-

D

C

C

Y

H

L

E

S

S

L

V

D

D

K

E

G

A

-

Y

-

E

-

D

-

G

-

K

-

N

-

Q

-

L

-

E

-

A

-

L

-

S

-

R

-

L

-

H

-

S

-

V

S

N

V

V

P

P

R

T

P

L

V

T

A

A

A

G

E

S

W

V

G

K

T

L

D

N

L

V

A

G

A

Q

P

F

E

G

A

S

S

A

E

L

P

Q

Q

K

M

S

Y

S

P

M

E

S

R

R

D

E

D

D

Y

Y

L

K

R

K

A

A

V

V

E

V

K

Q

T

A

K

K

E

E

L

H

I

I

A

L

D

A

G

G

E

D

C

I

I

F

Q

Q

A

V

V

V

L

L

S

S

T

Q

R

R

F

F

T

E

V

R

P

R

L

T

P

F

D

A

D
|
D

P

P

F

F

R

E

I

V

Y

Y

R

R

R

A

L

L

R

R

Q

I

A

V

N

N

P

P

S

S

P

P

F

Y

M

M

F

A

Y

Y

M

L

K

Q

L

A

P

R

G

G

C

C

D

-

G

-

T

I

L

L

L

V

G

A

S

S

P

P

E

E

M

I

M

L

V

T

R

R

C

V

E

E

E

K

G

R

R

T

L

I

E

V

V

N

R

R

P

P

I

L

A

A

G

G

T

T

R

I

P

R

R

R

G

G

G

K

N

S

E

K

S

A

E

E

D

D

L

K

A

V

L

L

A

E

D

Q

E

L

L

L

A

S

D

D

P

G

K

K

E

Q

R

C

A

A

E

E

H

H

V

I

M

M

L

L

V

V

D

D

L

L

G

G

R

R

N

N

D

D

L

V

G

G

R

K

I

V

S

S

N

K

P

P

G

G

T

S

V

V

E

K

V

V

K

E

R

K

F

L

M

M

N

N

V

V

E

E

R

R

F

Y

S

S

H

H

V

V

M

M

H

H

L

I

T

S

S

S

Y

T

V

V

E

T

G

G

E

E

L

L

Q

R

D

D

G

D

L

L

D

T

A

C

V

W

D

D

V

A

L

L

R

R

A

A

T

A

F

L

P

P

A

V

G

G

T

T

L

V

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

E

I

L

I

I

S

D

E

Q

M

M

E

E

P

G

Q

K

E

M

R

R

G

G

P

P

Y

Y

G

S

G

G

C

G

I

F

G

G

F

G

L

V

G

S

L

F

D

R

S

G

D

D

S

-

V

-

S

-

L

M

D

D

T

I

G

A

I

L

T

A

I

L

R

R

S

T

L

I

W

V

V

F

R

P

N

T

G

G

Q

S

-

R

-

F

-

D

-

T

-

M

-

Y

-

S

-

Y

-

T

-

D

-

K

-

N

-

A

-

R

-

Q

-

E

-

W

-

V

C

A

H

H

W

L

Q

Q

A

A

G

G

A

A

G

G

I

I

V

V

H

A

D

D

S

S

V

K

P

P

E

D

K

D

E

E

W

H

E

Q

E

E

C

C

R

L

N

N

K

K

A

A

R

A

V

G

L

L

M

A

E

R

V

A

I

I

N

D

D323 (= D231) mutation to N: Insensitive to feedback inhibition by tryptophan.

P32068 Anthranilate synthase alpha subunit 1, chloroplastic; Anthranilate synthase component 1-1; Anthranilate synthase component I-1; Protein A-METHYL TRYPTOPHAN RESISTANT 1; Protein JASMONATE-INDUCED DEFECTIVE LATERAL ROOT 1; Protein TRYPTOPHAN BIOSYNTHESIS 5; Protein WEAK ETHYLENE INSENSITIVE 2; EC 4.1.3.27 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
37% identity, 95% coverage: 15:463/474 of query aligns to 88:586/595 of P32068

query
sites
P32068

A

S

D

D

V

Q

Q

L

T

T

P

P

I

V

S

L

L

A

Y

Y

L

R

G

C

L

L

V

V

G

K

D

E

-

D

-

R

-

E

A

A

P

P

G

S

I

F

L

L

L

F

E

E

S

S

A

V

E

E

V

P

D

G

G

S

R

Q

L

M

-

S

-

V

G

G

R

R

Y

Y

T

S

L

V

I

V

A

G

W

A

D

Q

F

P

R

A

L

M

S

E

L

I

-

V

R

A

P

K

E

E

N

N

G

K

K

V

L

I

A

V

V

M

E

D

A

H

T

N

D

N

E

E

R

T

L

M

K

T

P

E

L

-

E

-

K

-

F

-

S

-

G

-

M

-

N

E

F

F

L

V

D

E

G

D

L

P

R

M

R

E

V

I

L

P

R

R

A

K

V

I

A

S

V

E

E

K

-

W

-

N

-

P

-

D

-

P

Q

Q

E

L

T

V

D

Q

D

D

G

L

P

P

L

-

P

D

A

A

L

F

T

C

R

G

G

G

L

W

Y

V

G

G

Y

F

L

F

G

S

Y

Y

G

D

I

T

A

V

G

R

M

Y

L

V

E

E

P

K

-

R

K

K

L

L

A

P

E

F

S

S

V

K

K

A

P

P

E

E

D

D

-

D

-

R

-

N

-

L

A

P

E

D

A

M

C

H

L

L

N

G

L

L

P

Y

G

D

Q

D

M

V

V

V

L

V

F

F

D

D

H

H

L

V

R

E

-

K

-

A

H

Y

S

V

C

I

C

H

Y

W

L

I

S

R

L

L

D

D

K

-

G

G

S

S

V

L

P

P

R

Y

P

E

V

K

A

A

A

Y

E

S

W

N

G

G

T

M

-

Q

-

H

-

L

-

E

-

N

-

L

-

V

-

A

-

K

-

L

-

H

D

D

L

I

A

E

A

P

P

P

E

K

A

L

S

A

-

A

-

G

-

N

-

V

-

N

-

L

-

Q

E

T

P

R

Q

Q

M

F

Y

G

P

P

E

S

R

L

D

D

D

N

-

S

-

N

-

V

-

T

-

C

-

E

Y

Y

L

K

R

E

A

A

V

V

E

V

K

K

T

A

K

K

E

E

L

H

I

I

A

L

D

A

G

G

E

D

C

I

I

F

Q

Q

A

I

V

V

L

L

S

S

T

Q

R

R

F

F

T

E

V

R

P

R

L

T

P

F

D

A

D
|
D

P

P

F

F

R

E

I

V

Y

Y

R

R

R

A

L

L

R

R

Q

V

A

V

N

N

P

P

S

S

P

P

F

Y

M

M

F

G

Y

Y

M

L

K

Q

L

A

P

R

G

G

C

C

D

-

G

-

T

I

L

L

L

V

G

A

S

S

P

P

E

E

M

I

M

L

V

T

R

K

C

V

E

K

E

Q

G

N

R

K

L

I

E

V

V

N

R

R

P

P

I

L

A

A

G

G

T

T

R

S

P

K

R

R

G

G

G

K

N

N

E

E

S

V

E

E

D

D

L

K

A

R

L

L

A

E

D

K

E

E

L

L

A

E

D

N

P

E

K

K

E

Q

R

C

A

A

E

E

H

H

V

I

M

M

L

L

V

V

D

D

L

L

G

G

R

R

N

N

D

D

L

V

G

G

R

K

I

V

S

T

N

K

P

Y

G

G

T

S

V

V

E

K

V

V

K

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R

K

F

L

M

M

N

N

V

I

E

E

R

R

F

Y

S

S

H

H

V

V

M

M

H

H

L

I

T

S

S

S

Y

T

V

V

E

T

G

G

E

E

L

L

Q

Q

D

D

G

G

L

L

D

T

A

C

V

W

D

D

V

V

L

L

R

R

A

A

T

A

F

L

P

P

A

V

G

G

T

T

L

V

S

S

G

G

A

A

P

P

K

K

V

V

R

K

A

A

M

M

E

E

I

L

I

I

S

D

E

E

M

L

E

E

P

P

Q

T

E

R

R

R

G

G

P

P

Y

Y

G

S

G

G

C

G

I

F

G

G

F

G

L

V

G

S

L

F

D

T

S

G

D

D

-

M

-

D

-

I

S

A

V

L

S

S

L

L

D

R

T

T

G

I

I

V

-

F

-

P

-

T

-

A

-

C

-

Q

-

Y

-

N

T

T

I

M

R

Y

S

S

L

Y

W

K

V

D

R

A

N

N

G

K

Q

R

C

R

H

E

W

W

-

V

-

A

-

Y

-

L

Q

Q

A

A

G

G

A

A

G

G

I

V

V

V

H

A

D

D

S

S

V

D

P

P

E

Q

K

D

E

E

W

H

E

C

E

E

C

C

R

Q

N

N

K

K

A

A

R

A

V

G

L

L

M

A

E

R

V

A

I

I

N

D

D341 (= D231) mutation to N: In trp5-1; insensitive to feedback inhibition by tryptophan and resistance to the herbicide 6-methylanthranilate.

O94582 Probable anthranilate synthase component 1; Anthranilate synthase component I; EC 4.1.3.27 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 94% coverage: 13:456/474 of query aligns to 28:468/489 of O94582

query
sites
O94582

L

I

P

P

A

A

D

D

V

M

Q

L

T

T

P

P

I

S

S

V

L

A

Y

Y

L

L

G

K

L

L

-

N

V

Q

G

G

D

K

A

K

P

Y

G

S

I

F

L

I

L

L

E

E

S

S

A

V

E

T

V

Q

D

G

G

E

R

S

L

V

G

S

R

R

Y

Y

T

S

L

F

I

I

A

G

W

S

D

P

F

Y

R

R

L

I

S

L

L

M

R

-

P

-

E

A

N

N

G

G

K

K

L

T

A

-

V

-

E

-

A

-

T

-

D

-

E

-

R

-

L

-

K

-

P

-

L

-

E

-

K

-

F

-

S

-

G

-

M

-

N

-

F

-

L

-

D

D

G

P

L

L

R

A

R

R

V

L

L

E

R

R

A

E

V

L

A

-

V

-

E

-

Q

K

E

E

T

V

D

K

D

T

G

A

P

P

-

V

-

E

-

G

L

L

P

P

A

S

L

F

T

S

R

G

G

G

L

A

Y

V

G

G

Y

Y

L

V

G

S

Y

Y

G

D

I

C

A

I

G

K

M

Y

L

F

E

E

P

P

K

-

L

-

A

T

E

T

S

E

V

M

K

P

P

L

E

E

D

D

A

T

-

L

-

G

-

L

-

P

E

E

A

A

C

M

L

F

N

F

L

M

P

T

G

D

Q

D

M

L

V

V

L

A

F

F

D

D

H

H

L

A

R

Y

H

Q

S

T

C

V

C

K

Y

I

L

I

S

S

L

H

D

V

K

C

G

I

S

Q

V

Q

P

G

R

R

P

P

V

I

-

E

-

A

-

Y

-

E

A

A

E

V

W

F

G

K

T

I

D

N

L

M

A

L

A

K

P

K

E

K

A

L

S

E

E

S

P

P

Q

E

M

I

-

P

Y

L

P

P

E

E

R

Q

-

K

-

V

-

H

-

L

-

G

-

Y

-

E

-

A

-

K

-

S

-

N

-

V

-

G

-

E

D

D

D

G

Y

Y

L

K

R

A

A

F

V

V

E

S

K

N

T

L

K

K

E

E

L

H

I

I

A

F

D

N

G

G

E

D

C

I

I

F

Q

Q

A

A

V

V

L

P

S

S

T

Q

R

R

F

I

T

A

V

R

P

R

L

T

P

D

D

L

D

H

P

P

F

F

R

N

I

L

Y

Y

R

R

R

H

L

L

R

R

Q

T

A

V

N

N

P

P

S

S

P

P

F

Y

M

M

F

F

Y

Y

M

I

K

H

L

C

P

D

G

D

C

F

D

D

G

-

T

-

L

I

G

G

S

A

S

S

P

P

E

E

M

L

V

V

R

K

C

S

E

E

E

H

G

G

R

R

L

I

E

I

V

N

R

H

P

P

I

I

A

A

G

G

T

T

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V

P

P

R

R

G

G

G

K

N

T

E

K

S

E

E

E

D

D

L

E

A

A

L

Y

A

A

D

K

E

D

L

L

A

A

D

S

P

V

K

K

E

D

R

R

A

A

E

E

H

H

V

V

M

M

L

L

V

V

D

D

L

L

G

A

R

R

N

N

D

D

L

V

G

S

R

R

I

V

S

C

N

D

P

L

G

D

T

T

V

T

E

S

V

V

K

D

R

K

F

L

M

M

N

T

V

I

E

E

R

K

F

F

S

S

H

H

V

V

M

Q

H

H

L

L

T

V

S

S

Y

Q

V

V

E

S

G

G

E

V

L

L

Q

R

D

P

G

D

L

K

D

T

A

R

V

F

D

D

V

A

L

F

R

R

A

S

T

I

F

F

P

P

A

A

G

G

T

T

L

V

S
|
S

G

G

A
x
S

P

P

K

K

V

V

R

R

A

A

M

I

E

Q

I

L

I

V

S

Y

E

G

M

L

E

E

P

K

Q

E

E

K

R

R

G

G

P

I

Y

Y

G

A

G

G

C

A

I

V

G

G

F

R

L

W

G

G

L

Y

D

E

S

D

D

D

S

-

V

-

S

N

L

M

D

D

T

T

G

C

I

I

T

A

I

I

R

R

S

T

L

M

W

V

V

Y

R

K

N

D

G

G

Q

T

C

V

H

Y

W

L

Q

Q

A

A

G

G

A

G

G

G

I

I

V

V

H

F

D

D

S

S

V

D

P

E

E

Q

K

D

E

E

W

Y

E

V

E

E

C

T

R

L

N

N

K

K

A

L

R

R

S390 (= S376) modified: Phosphoserine
S392 (≠ A378) modified: Phosphoserine

Sites not aligning to the query:

488 modified: Phosphoserine

P00898 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
48% identity, 56% coverage: 197:462/474 of query aligns to 243:510/520 of P00898

query
sites
P00898

D

D

D

A

Y

F

L

G

R

A

A

V

V

V

E

R

K

Q

T

L

K

Q

E

K

L

A

I

I

A

R

D

A

G

G

E

E

C

I

I

F

Q

Q

A

V

V

V

L

P

S

S

T

R

R

R

F

F

T

S

V

L

P

P

L

C

P

P

D

S

D

-

P

P

F

L

R

A

I

A

Y

Y

R

Y

R

V

L

L

R

K

Q

K

A

S

N
|
N

P
|
P

S

S

P

P

F

Y

M
|
M

F
|
F

Y

F

M

M

K

Q

L

-

P

-

G

D

C

N

D

D

G

F

T

T

L

L

L

F

G
|
G

S

A

S

S

P

P

E

E

M

S

V

L

R

K

C

Y

E

D

E

A

G

A

-

S

-

R

R

Q

L

I

E

E

V

I

R

Y

P

P

I

I

A

A

G

G

T

T

R

R

P

P

R

R

G

G

-

R

-

A

-

D

G

G

N

T

E

L

S

D

E

R

D

D

L

L

A

D

L

S

A

R

D

I

E

E

L

L

-

D

-

M

L

R

A

T

D

D

P

H

K

K

E

E

R

L

A

S

E

E

H

H

V

L

M

M

L

L

V

V

D

D

L

L

G

A

R

R

N

N

D

D

L

L

G

A

R

R

I

I

S

C

N

T

P

P

G

G

T

S

V

R

E

Y

V

V

K

A

R

D

F

L

M

T

N

K

V

V

E

D

R

R

F

Y

S

S

H

Y

V

V

M

M

H

H

L

L

T

V

S

S

Y
x
R

V

V

E

V

G

G

E

E

L

L

Q

R

D

H

G

D

L

L

D

D

A

A

V

L

D

H

V

A

L

Y

R

R

A

A

T

C

F

M

P

N

A

M

G

G

T

T

L

L

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

Q

I

L

I

I

S

A

E

D

M

A

E

E

P

G

Q

Q

E

R

R

R

G

G

P

S

Y

Y

G

G

C

A

I

V

G

G

F

Y

L

F

G

T

L

A

D

H

S
x
G

D

D

S

-

V

-

S

-

L

L

D

D

T

T

G
x
C

I

I

T

V

I

I

R

R

S

S

L

A

W

L

V

V

R

E

N

N

G

G

Q

I

C

A

H

T

W

V

Q

Q

A

A

G

G

A

A

G

G

I

I

V

V

H

L

D

D

S

S

V

V

P

P

E

Q

K

S

E

E

W

A

E

D

E

E

C

T

R

R

N

N

K

K

A

A

R

R

V

A

L

V

M

L

E

R

V

A

I

I

N288 (= N243) mutation to D: Decrease in feedback control by tryptophan.
P289 (= P244) mutation to L: Decrease in feedback control by tryptophan.
M293 (= M248) mutation to T: Complete loss of feedback control by tryptophan.
F294 (= F249) mutation to L: Decrease in feedback control by tryptophan.
G305 (= G262) mutation to S: Decrease in feedback control by tryptophan.
R402 (≠ Y351) mutation to W: Almost no change in feedback control by tryptophan.
G460 (≠ S409) mutation to D: Almost no change in feedback control by tryptophan.
C465 (≠ G417) mutation to Y: Complete loss of feedback control by tryptophan. 4-fold decrease of affinity binding for chorismate.

Sites not aligning to the query:

39 E→K: Complete loss of feedback control by tryptophan.
40 binding L-tryptophan; S→F: Complete loss of feedback control by tryptophan.
41 A→V: Decrease in feedback control by tryptophan.
50 binding L-tryptophan
128 R→H: Almost no change in feedback control by tryptophan.
174 C→Y: Almost no change in feedback control by tryptophan.
515 H→Y: Almost no change in feedback control by tryptophan.

1i1qA Structure of the cooperative allosteric anthranilate synthase from salmonella typhimurium (see paper)
48% identity, 56% coverage: 197:462/474 of query aligns to 239:506/512 of 1i1qA

query
sites
1i1qA

D

D

D

A

Y

F

L

G

R

A

A

V

V

V

E

R

K

Q

T

L

K

Q

E

K

L

A

I

I

A

R

D

A

G

G

E

E

C

I

I

F

Q
|
Q

A

V

V

V

L

P

S

S

T

R

R

R

F

F

T

S

V

L

P

P

L

C

P

P

D

S

D

-

P

P

F

L

R

A

I

A

Y

Y

R

Y

R

V

L

L

R

K

Q

K

A

S

N

N

P

P

S

S

P
|
P

F
x
Y

M
|
M

F

F

Y

F

M

M

K

Q

L

-

P

-

G

D

C

N

D

D

G

F

T

T

L

L

L

F

G

G

S

A

S

S

P

P

E
|
E

M

S

V

L

R

K

C

Y

E

D

E

A

G

A

-

S

-

R

R

Q

L

I

E

E

V

I

R

Y

P

P

I

I

A
|
A

G

G

T

T

R

R

P

P

R

R

G

G

-

R

-

A

-

D

G

G

N

T

E

L

S

D

E

R

D

D

L

L

A

D

L

S

A

R

D

I

E

E

L

L

-

D

-

M

L

R

A

T

D

D

P

H

K

K

E

E

R

L

A

S

E
|
E

H

H

V

L

M

M

L

L

V

V

D

D

L

L

G

A

R

R

N

N

D

D

L

L

G

A

R

R

I

I

S

C

N

T

P

P

G

G

T

S

V

R

E

Y

V

V

K

A

R

D

F

L

M

T

N

K

V

V

E

D

R

R

F

Y

S

S

H

Y

V

V

M

M

H
|
H

L

L

T

V

S

S

Y

R

V

V

E

V

G

G

E

E

L

L

Q

R

D

H

G

D

L

L

D

D

A

A

V

L

D

H

V

A

L

Y

R

R

A

A

T

C

F

M

P

N

A

M

G

G

T
|
T

L

L

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

Q

I

L

I

I

S

A

E

D

M

A

E

E

P

G

Q

Q

E

R

R

R

G

G

P

S

Y
|
Y

G

G

C

A

I

V

G
|
G

F

Y

L

F

G

T

L

A

D

H

S

G

D

D

S

-

V

-

S

-

L

L

D

D

T

T

G
x
C

I

I

T

V

I

I

R
|
R

S

S

L

A

W

L

V

V

R

E

N

N

G

G

Q

I

C

A

H

T

W

V

Q

Q

A

A

G

G

A

A

G
|
G

I

I

V

V

H

L

D

D

S

S

V

V

P

P

E

Q

K

S

E

E

W

A

E

D

E
|
E

C

T

R

R

N

N

K
|
K

A

A

R

R

V

A

L

V

M

L

E

R

V

A

I

I

active site: Q259 (= Q217), E305 (= E266), A323 (= A282), E357 (= E310), H394 (= H347), T421 (= T374), Y445 (= Y398), R465 (= R421), G481 (= G437), E494 (= E450), K498 (= K454)
binding tryptophan: P287 (= P246), Y288 (≠ F247), M289 (= M248), G450 (= G403), C461 (≠ G417)

Sites not aligning to the query:

binding tryptophan: 34, 35, 36, 46

1i7qA Anthranilate synthase from s. Marcescens (see paper)
33% identity, 95% coverage: 20:471/474 of query aligns to 18:517/517 of 1i7qA

query
sites
1i7qA

P

P

I

T

S

T

L

L

Y

F

L

H

G

Q

L

L

V

C

G

G

D

A

A

R

P

P

G

A

-

T

I

L

L

L

E

E

S

S

A

A

E

E

V

I

D

N

G

D

R

K

L

Q

G

N

R

L

Y

Q

T

S

L

L

I

L

A

V

W

I

D

D

F

S

R

A

L

L

S

R

L

I

-

T

-

A

-

L

-

G

-

H

-

T

-

V

-

S

-

V

-

Q

-

A

-

L

-

T

-

A

-

N

-

G

-

P

-

A

-

L

-

P

-

L

-

D

-

E

-

A

-

L

-

P

-

E

-

V

-

R

-

N

-

Q

-

A

R

R

P

P

E

N

N

G

G

R

K

E

L

L

A

T

V

F

E

P

A

A

T

I

D

D

E

A

R

V

L

Q

K

D

P

E

L

D

E

A

K

R

F

L

S

R

G

S

M

L

N

S

F

V

L

F

D

D

G

A

L

L

R

R

R

T

V

I

L

L

R

T

A

L

V

V

-

D

-

S

-

P

A

A

V

D

E

E

Q

R

E

E

T

A

D

-

G

-

P

-

L

-

P

-

A

V

L

M

T

L

R

G

G

G

L

L

Y

F

G

A

Y

Y

-

D

L

L

G

V

Y

A

G

G

I

F

A

E

G

N

M

L

L

-

E

-

P

P

K

A

L

L

A

R

E

Q

S

D

V

Q

K

R

P

C

E

P

D

D

A

F

E

-

A

-

C

C

L

F

N

Y

L

L

P

A

G

E

Q

T

M

L

V

L

L

V

F

L

D

D

H

H

L

Q

R

R

H

G

S

S

C

-

Y

-

L

-

S

-

L

-

D

-

K

-

G

-

S

-

V

-

P

-

R

-

P

-

V

-

A

-

A

A

E

R

W

L

G

Q

T

A

D

S

L

V

A

F

A

S

P

E

E

Q

A

A

S

S

E

E

P

A

Q

Q

M

R

Y

L

P

Q

E

H

R

R

D

L

D

E

Y

Q

L

L

R

Q

A

A

-

E

-

L

-

Q

-

P

-

Q

-

P

-

I

-

P

-

H

-

Q

-

K

-

L

-

E

-

N

-

M

-

Q

-

L

-

S

-

C

-

N

-

Q

-

S

-

D

-

E

-

Y

-

G

-

A

-

V

V

V

E

S

K

E

T

L

K

Q

E

E

L

A

I

I

A

R

D

Q

G

G

E

E

C

I

I

F

Q
|
Q

A

V

V

V

L

P

S

S

T

R

R

R

F

F

T

S

V

L

P

P

L

C

P

P

D

A

D

-

P

P

F

L

R

G

I

P

Y

Y

R

Q

R

T

L

L

R

K

Q

D

A

N

N

N

P

P

S

S

P

P

F

Y

M

M

F

F

Y

F

M

M

K

Q

L

-

P

-

G

D

C

D

D

D

G

F

T

T

L

L

L

F

G

G

S

A

S

S

P

P

E
|
E

M

S

M

A

V

L

R

K

C

Y

E

D

E

A

G

G

-

N

-

R

R

Q

L

I

E

E

V

I

R

Y

P

P

I

I

A
|
A

G

G

T

T

R

R

P

P

R

R

G

G

-

R

G

A

N

D

E

G

S

S

E

L

D

D

L

L

A

D

L

L

A

D

D

S

-

R

-

I

-

E

-

L

E

E

L

M

L

R

A

T

D

D

P

H

K

K

E

E

R

L

A

A

E
|
E

H

H

V

L

M

M

L

L

V

V

D

D

L

L

G

A

R

R

N

N

D

D

L

L

G

A

R

R

I

I

S

C

N

Q

P

A

G

G

T

S

V

R

E

Y

V

V

K

A

R

D

F

L

M

T

N

K

V

V

E

D

R

R

F

Y

S

S

H

F

V

V

M

M

H
|
H

L

L

T

V

S

S

Y

R

V

V

E

V

G

G

E

T

L

L

Q

R

D

A

G

D

L

L

D

D

A

V

V

L

D

H

V

A

L

Y

R

Q

A

A

T

C

F

M

P

N

A

M

G

G

T
|
T

L

L

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

Q

I

L

I

I

S

A

E

A

M

L

E

R

P

S

Q

T

E

R

R

R

G

G

P

S

Y
|
Y

G

G

C

R

I

V

G

G

F

Y

L

F

G

-

L

-

D

-

S

T

D

A

S

V

V

R

S

N

L

L

D

D

T

T

G

C

I

I

T

V

I
|
I

R
|
R

S

S

L

A

W

Y

V

V

R

E

N

D

G

G

Q

H

C

R

H

T

W

V

Q

Q

A
|
A

G
|
G

A

A

G
|
G

I

V

V

V

H

Q

D

D

S

S

V

I

P

P

E

E

K

R

E

E

W

A

E

D

E
|
E

C

T

R

R

N

N

K
|
K

A

A

R

R

-

A

V

V

L

L

M

R

E

A

V

I

I

A

N

T

G

A

R

H

G

H

G

A

T

K

D

E

V

V

F

F

active site: Q260 (= Q217), E306 (= E266), A324 (= A282), E358 (= E310), H395 (= H347), T422 (= T374), Y446 (= Y398), R466 (= R421), G482 (= G437), E495 (= E450), K499 (= K454)
binding magnesium ion: E358 (= E310), E495 (= E450)
binding pyruvic acid: Y446 (= Y398), I465 (= I420), R466 (= R421), A479 (= A434), G480 (= G435), K499 (= K454)

1i7sA Anthranilate synthase from serratia marcescens in complex with its end product inhibitor l-tryptophan (see paper)
37% identity, 85% coverage: 67:471/474 of query aligns to 101:511/511 of 1i7sA

query
sites
1i7sA

A

A

V

V

E

Q

A

D

T

E

D

D

E

A

R

R

L

L

K

R

P

S

L

L

E

S

K

V

F

F

S

-

G

-

M

-

N

-

F

-

L

-

D

D

G

A

L

L

R

R

R

T

V

I

L

L

R

T

A

L

V

V

-

D

-

S

-

P

A

A

V

D

E

E

Q

R

E

E

T

A

D

-

G

-

P

-

L

-

P

-

A

V

L

M

T

L

R

G

G

G

L

L

Y

F

G

A

Y

Y

-

D

L

L

G

V

Y

A

G

G

I

F

A

E

G

N

M

L

L

-

E

-

P

P

K

A

L

L

A

R

E

Q

S

D

V

Q

K

R

P

C

E

P

D

D

A

F

E

-

A

-

C

C

L

F

N

Y

L

L

P

A

G

E

Q

T

M

L

V

L

L

V

F

L

D

D

H

H

L

Q

R

R

H

G

S

S

C

-

Y

-

L

-

S

-

L

-

D

-

K

-

G

-

S

-

V

-

P

-

R

-

P

-

V

-

A

-

A

A

E

R

W

L

G

Q

T

A

D

S

L

V

A

F

A

S

P

E

E

Q

A

A

S

S

E

E

P

A

Q

Q

M

R

Y

L

P

Q

E

H

R

R

D

L

D

E

Y

Q

L

L

R

Q

A

A

-

E

-

L

-

Q

-

P

-

Q

-

P

-

I

-

P

-

H

-

Q

-

K

-

L

-

E

-

N

-

M

-

Q

-

L

-

S

-

C

-

N

-

Q

-

S

-

D

-

E

-

Y

-

G

-

A

-

V

V

V

E

S

K

E

T

L

K

Q

E

E

L

A

I

I

A

R

D

Q

G

G

E

E

C

I

I

F

Q
|
Q

A

V

V

V

L

P

S

S

T

R

R

R

F

F

T

S

V

L

P

P

L

C

P

P

D

A

D

-

P

P

F

L

R

G

I

P

Y

Y

R

Q

R

T

L

L

R

K

Q

D

A

N

N

N

P

P

S

S

P
|
P

F
x
Y

M
|
M

F

F

Y

F

M

M

K

Q

L

-

P

-

G

D

C

D

D

D

G

F

T

T

L

L

L

F

G

G

S

A

S

S

P

P

E
|
E

M

S

M

A

V

L

R

K

C

Y

E

D

E

A

G

G

-

N

-

R

R

Q

L

I

E

E

V

I

R

Y

P

P

I

I

A
|
A

G

G

T

T

R

R

P

P

R

R

G

G

-

R

G

A

N

D

E

G

S

S

E

L

D

D

L

L

A

D

L

L

A

D

D

S

-

R

-

I

-

E

-

L

E

E

L

M

L

R

A

T

D

D

P

H

K

K

E

E

R

L

A

A

E
|
E

H

H

V

L

M

M

L

L

V

V

D

D

L

L

G

A

R

R

N

N

D

D

L

L

G

A

R

R

I

I

S

C

N

Q

P

A

G

G

T

S

V

R

E

Y

V

V

K

A

R

D

F

L

M

T

N

K

V

V

E

D

R

R

F

Y

S

S

H

F

V

V

M

M

H
|
H

L

L

T

V

S

S

Y

R

V

V

E

V

G

G

E

T

L

L

Q

R

D

A

G

D

L

L

D

D

A

V

V

L

D

H

V

A

L

Y

R

Q

A

A

T

C

F

M

P

N

A

M

G

G

T
|
T

L

L

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

Q

I

L

I

I

S

A

E

A

M

L

E

R

P

S

Q

T

E

R

R

R

G

G

P

S

Y
|
Y

G

G

C

R

I
x
V

G
|
G

F

Y

L

F

G

-

L

-

D

-

S

T

D

A

S

V

V

R

S

N

L

L

D
|
D

T

T

G
x
C

I

I

T

V

I

I

R
|
R

S

S

L

A

W

Y

V

V

R

E

N

D

G

G

Q

H

C

R

H

T

W

V

Q

Q

A

A

G

G

A

A

G
|
G

I

V

V

V

H

Q

D

D

S

S

V

I

P

P

E

E

K

R

E

E

W

A

E

D

E
|
E

C

T

R

R

N

N

K
|
K

A

A

R

R

-

A

V

V

L

L

M

R

E

A

V

I

I

A

N

T

G

A

R

H

G

H

G

A

T

K

D

E

V

V

F

F

active site: Q254 (= Q217), E300 (= E266), A318 (= A282), E352 (= E310), H389 (= H347), T416 (= T374), Y440 (= Y398), R460 (= R421), G476 (= G437), E489 (= E450), K493 (= K454)
binding tryptophan: P282 (= P246), Y283 (≠ F247), M284 (= M248), V444 (≠ I402), G445 (= G403), D454 (= D415), C456 (≠ G417)

Sites not aligning to the query:

binding tryptophan: 35, 36, 37

P05041 Aminodeoxychorismate synthase component 1; ADC synthase; ADCS; 4-amino-4-deoxychorismate synthase component 1; EC 2.6.1.85 from Escherichia coli (strain K12) (see 4 papers)
33% identity, 94% coverage: 18:461/474 of query aligns to 15:451/453 of P05041

query
sites
P05041

Q

Q

T

D

P

A

I

A

S

E

L

F

Y

Y

L

F

G

S

L

R

V

L

G

S

D

H

A

L

P

P

-

W

G

A

I

M

L

L

E

H

S
|
S

A

G

E

Y

V

A

D

D

G

H

R

P

L

Y

G

S

R

R

Y

F

T

D

L

I

I

V

A

V

W

A

D

E

F

P

R

I

L

C

S

T

L

L

R

T

P

T

E

F

N

G

G

K

K

E

L

T

A

V

V

V

E

S

A

E

T

S

D

E

E

K

R

R

L

T

K

T

P

T

L

T

E

D

K

-

F

-

S

-

G

-

M

-

N

D

F

P

L

L

D

Q

G

V

L

L

R

Q

V

V

L

L

-

D

R

R

A

A

V

D

A

I

V

R

E

P

Q

T

E

H

T

N

D

E

D

D

G

L

P

P

L

F

P

Q

A

G

L

G

T

A

R

L

G

G

L

L

Y

F

G

G

Y

Y

-

D

L

L

G

G

Y

R

G

R

I

F

A

E

G

S

M

L

L

-

E

-

P

-

K

-

L

-

A

-

E

-

S

-

V

-

K

-

P

P

E

E

D

I

A

A

E

E

A

Q

C

D

L

I

N

V

L

L

P

P

G

D

Q

M

M

A

V

V

-

G

-

I

-

Y

-

D

-

W

-

A

-

L

L

I

F

V

D

D

H

H

L

Q

R

R

H

H

S

T

C

V

C

S

Y

L

L

L

S

S

L

H

D

N

K

D

G

V

S

N

V

A

P

R

R

R

P

-

V

-

A

-

A

A

E

W

W

L

G

E

T

S

D

Q

L

Q

A

F

A

S

P

P

E

Q

-

E

-

D

-

F

-

T

-

L

A

T

S

S

E

D

P

W

Q

Q

M

S

Y

N

P

M

E

T

R

R

D

E

D

Q

Y

Y

L

G

R

E

A

K

V

F

E

R

K

Q

T

V

K

Q

E

E

L

Y

I

L

A

H

D

S

G

G

E

D

C

C

I

Y

Q

Q

A

V

V

N

L

L

S

A

T

Q

R

R

F

F

T

H

V

A

P

T

L

Y

P

S

D

G

D

D

P

E

F

W

R

Q

I

A

Y

F

R

L

R

Q

L

L

R

N

Q

Q

A

A

N

N

P

R

S

A

P

P

F

F

M

S

F

A

Y

F

M

L

K

R

L

L

P

E

G

-

C

-

D

Q

G

G

T

A

L

I

L

L

G

S

S

L

S

S

P

P

E
|
E

M

R

M

F

V

I

R

L

C

C

E

D

E

N

G

S

R

E

L

I

E

Q

V

T

R

R

P

P

I

I

A
x
K

G
|
G

T

T

R

L

P

P

R

R

G

L

G

P

N

D

E

P

S

Q

E

E

D

D

L

S

A

K

L

Q

A

A

D

V

E

K

L

L

L

A

A

N

D

S

P

A

K

K

E

D

R

R

A

A

E

E

H

N

V

L

M

M

L

I

V

V

D

D

L

L

G

M

R
|
R

N

N

D

D

L

I

G

G

R
|
R

I

V

S

A

N

V

P

A

G

G

T
x
S

V

V

E

K

V

V

K

P

R

E

F

L

M

F

N

V

V

V

E

E

R

P

F

F

S

P

H

A

V

V

M

H

H
|
H

L

L

T

V

S

S

Y

T

V

I

E

T

G

A

E

Q

L

L

Q

P

D

E

G

Q

L

L

D

H

A

A

V

S

D

D

V

L

L

L

R

R

A

A

T

A

F

F

P

P

A

G

G

G

T

S

L

I

S

T

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

E

I

I

S

D

E

E

M

L

E

E

P

P

Q

Q

E

R

R

R

G

N

P

A

Y

W

G

C

G

G

C

S

I

I

G

G

F

Y

L

L

G

S

L

F

D

C

S

G

D

N

S

-

V

-

S

-

L

M

D

D

T

T

G

S

I

I

T

T

I

I

R

R

S

T

L

L

W

T

V

A

R

I

N

N

G

G

Q

Q

C

I

H

F

W

C

Q

S

A

A

G

G

A

G

G

G

I

I

V

V

H

A

D

D

S

S

V

Q

P

E

E

E

K

A

E

E

W

Y

E

Q

E

E

C

T

R

F

N

D

K

K

A

V

-

N

R

R

V

I

L

L

M

K

E

Q

V

L

S36 (= S38) binding L-tryptophan
E258 (= E266) mutation to A: The reaction is extremely slow.; mutation to D: The reaction is extremely slow.
K274 (≠ A282) mutation to A: Absence of covalent intermediate. Addition of ammonia allows the formation of the covalent intermediate and shows that ammonia can replace the function of K-274. Reduced catalytic efficiency.; mutation to R: Absence of covalent intermediate.; mutation to R: Reduced catalytic efficiency.
G275 (= G283) mutation to S: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
R311 (= R319) mutation to K: Catalytically active in the NH3-dependent, but inactive for the glutamine-dependent reactions and fails to complex with PabA.
R316 (= R324) mutation to H: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.
S322 (≠ T330) mutation to T: Complete loss of aminodeoxychorismate synthase activity.
H339 (= H347) mutation to W: Catalytically inactive for both the glutamine-dependent and ammonia-dependent reactions and fails to interact with PabA.

P00897 Anthranilate synthase component 1; AS; ASI; EC 4.1.3.27 from Serratia marcescens (see paper)
32% identity, 95% coverage: 20:471/474 of query aligns to 20:519/519 of P00897

query
sites
P00897

P

P

I

T

S

T

L

L

Y

F

L

H

G

Q

L

L

V

C

G

G

D

A

A

R

P

P

G

A

-

T

I

L

L

L

E

E

S
|
S

A

A

E

E

V

I

D

N

G

D

R

K

L

Q

G

N

R

L

Y

Q

T

S

L

L

I

L

A

V

W

I

D

D

F

S

R

A

L

L

S

P

L

I

-

T

-

A

-

L

-

G

-

H

-

T

-

V

-

S

-

V

-

Q

-

A

-

L

-

T

-

A

-

N

-

G

-

P

-

A

-

L

-

P

-

V

-

L

-

D

-

E

-

A

-

L

-

P

-

E

-

V

-

R

-

N

-

Q

-

A

R

R

P

P

E

N

N

G

G

R

K

E

L

L

A

T

V

F

E

P

A

A

T

I

D

D

E

A

R

V

L

Q

K

D

P

E

L

D

E

A

K

R

F

L

S

R

G

S

M

L

N

S

F

V

L

F

D

D

G

A

L

L

R

R

R

T

V

L

L

L

R

T

A

L

V

V

A

D

V

S

E

P

Q

A

E

D

T

E

D

R

D

E

G

A

P

V

L

M

P

-

A

-

L

-

T

-

R

-

G

-

L

L

Y

G

G

G

Y

L

L

F

G

A

Y

Y

G

D

I

L

A

V

G

A

M

G

L

F

E

E

P

N

K

L

L

P

A

A

E

V

S

R

V

Q

K

D

P

Q

E

R

D

C

A

P

E

D

A

F

C

C

L

F

N

Y

L

L

P

A

G

E

Q

T

M

L

V

L

L

V

F

L

D

D

H

H

L

Q

R

R

H

G

S

S

C

-

Y

-

L

-

S

-

L

-

D

-

K

-

G

-

S

-

V

-

P

-

R

-

P

-

V

-

A

-

A

A

E

R

W

L

G

Q

T

A

D

S

L

V

A

F

A

S

P

E

E

Q

A

A

S

S

E

E

P

A

Q

Q

M

R

Y

L

P

Q

E

H

R

R

D

L

D

E

Y

Q

L

L

R

Q

A

A

-

E

-

L

-

Q

-

P

-

Q

-

P

-

I

-

P

-

H

-

Q

-

K

-

L

-

E

-

N

-

M

-

Q

-

L

-

S

-

C

-

N

-

Q

-

S

-

D

-

E

-

Y

-

G

-

A

-

V

V

V

E

S

K

E

T

L

K

Q

E

E

L

A

I

I

A

R

D

Q

G

G

E

E

C

I

I

F

Q

Q

A

V

V

V

L

P

S

S

T

R

R

R

F

F

T

S

V

L

P

P

L

C

P

P

D

A

D

-

P

P

F

L

R

G

I

P

Y

Y

R

Q

R

T

L

L

R

K

Q

D

A

N

N

N

P

P

S

S

P
|
P

F
x
Y

M
|
M

F

F

Y

F

M

M

K

Q

L

-

P

-

G

D

C

D

D

D

G

F

T

T

L

L

L

F

G

G

S

A

S

S

P

P

E

E

M

S

M

A

V

L

R

K

C

Y

E

D

E

A

G

G

-

N

-

R

R

Q

L

I

E

E

V

I

R

Y

P

P

I

I

A

A

G

G

T

T

R

R

P

P

R

R

G

G

-

R

G

A

N

D

E

G

S

S

E

L

D

D

L

L

A

D

L

L

A

D

D

S

-

R

-

I

-

E

-

L

E

E

L

M

L

R

A

T

D

D

P

H

K

K

E

E

R

L

A

A

E
|
E

H

H

V

L

M

M

L

L

V

V

D

D

L

L

G

A

R

R

N

N

D

D

L

L

G

A

R

R

I

I

S

C

N

Q

P

A

G

G

T

S

V

R

E

Y

V

V

K

A

R

D

F

L

M

T

N

K

V

V

E

D

R

R

F

Y

S

S

H

F

V

V

M

M

H

H

L

L

T

V

S

S

Y

R

V

V

E

V

G

G

E

T

L

L

Q

R

D

A

G

D

L

L

D

D

A

V

V

L

D

H

V

A

L

Y

R

Q

A

A

T

C

F

M

P

N

A

M

G

G

T

T

L

L

S

S

G

G

A

A

P

P

K

K

V

V

R

R

A

A

M

M

E

Q

I

L

I

I

S

A

E

A

M

L

E

R

P

S

Q

T

E

R

R

R

G

G

P

S

Y

Y

G

G

C

R

I

V

G

G

F

Y

L

F

G

-

L

-

D

-

S

T

D

A

S

H

V

R

S

H

L

L

D

D

T

T

G

C

I

I

T

V

I

I

R

R

S

S

L

A

W

Y

V

V

R

E

N

D

G

G

Q

H

C

R

H

T

W

V

Q

Q

A

A

G

G

A

A

G

G

I

V

V

V

H

Q

D

D

S

S

V

I

P

R

E

R

K

R

E

E

W

A

E

D

E
|
E

C

T

R

R

N

N

K

K

A

A

R

R

-

A

V

V

L

L

M

R

E

A

V

I

I

A

N

T

G

A

R

H

G

H

G

A

T

K

D

E

V

V

F

F

S39 (= S38) binding L-tryptophan
PYM 290:292 (≠ PFM 246:248) binding L-tryptophan
E360 (= E310) binding Mg(2+)
E497 (= E450) binding Mg(2+)

1k0eA The crystal structure of aminodeoxychorismate synthase from formate grown crystals (see paper)
32% identity, 94% coverage: 18:461/474 of query aligns to 13:435/437 of 1k0eA

query
sites
1k0eA

Q

Q

T

D

P

A

I

A

S

E

L

F

Y

Y

L

F

G

S

L

R

V

L

G

S

D

H

A

L

P

P

-

W

G

A

I

M

L

L

L
|
L

E
x
H

S
|
S

A

G

E

Y

V

A

D

D

G

H

R

P

L
x
Y

G

S

R

R

Y
x
F

T

D

L

I

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active site: E256 (= E266), K272 (≠ A282), E286 (= E310), H323 (= H347), S350 (≠ T374), W374 (≠ Y398), R394 (= R421), G410 (= G437), E423 (= E450), K427 (= K454)
binding tryptophan: L32 (= L36), H33 (≠ E37), S34 (= S38), Y41 (≠ L45), F44 (≠ Y48), P238 (= P246), F239 (= F247), S240 (≠ M248)

1k0gA The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
30% identity, 94% coverage: 18:461/474 of query aligns to 15:418/420 of 1k0gA

query
sites
1k0gA

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active site: E258 (= E266), K274 (= K306), E278 (= E310), S333 (≠ T374), W357 (≠ Y398), R377 (= R421), G393 (= G437), E406 (= E450), K410 (= K454)
binding phosphate ion: D113 (vs. gap), R116 (≠ Y121), D347 (≠ S388), R353 (≠ E394)
binding tryptophan: L34 (= L36), H35 (≠ E37), S36 (= S38), Y43 (≠ L45), S44 (≠ G46), F46 (≠ Y48), P240 (= P246), F241 (= F247), S242 (≠ M248)

1k0gB The crystal structure of aminodeoxychorismate synthase from phosphate grown crystals (see paper)
30% identity, 94% coverage: 18:461/474 of query aligns to 15:415/415 of 1k0gB

query
sites
1k0gB

Q

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P

A

Y
x
W

G

C

G

G

C

S

I

I

G

G

F

Y

L

L

G

S

L

F

D

C

S

G

D

N

S

-

V

-

S

-

L

M

D

D

T

T

G

S

I

I

T

T

I

I

R
|
R

S

T

L

L

W

T

V

A

R

I

N

N

G

G

Q

Q

C

I

H

F

W

C

Q

S

A

A

G

G

A

G

G
|
G

I

I

V

V

H

A

D

D

S

S

V

Q

P

E

E

E

K

A

E

E

W

Y

E

Q

E
|
E

C

T

R

F

N

D

K
|
K

A

V

-

N

R

R

V

I

L

L

M

K

E

Q

V

L

active site: E258 (= E266), K274 (≠ A282), E277 (= E310), S330 (≠ T374), W354 (≠ Y398), R374 (= R421), G390 (= G437), E403 (= E450), K407 (= K454)
binding phosphate ion: Y112 (= Y118), D113 (vs. gap), R116 (≠ Y121), D344 (≠ S388), R350 (≠ E394)
binding tryptophan: L34 (= L36), H35 (≠ E37), S36 (= S38), Y43 (≠ L45), S44 (≠ G46), R45 (= R47), F46 (≠ Y48), P240 (= P246), F241 (= F247)

8hx8A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae co-crystallized with chorismate (see paper)
33% identity, 74% coverage: 114:464/474 of query aligns to 308:672/673 of 8hx8A

query
sites
8hx8A

G

G

L

Y

Y

V

G

G

Y

Y

L

L

G

G

Y

Y

G

E

I

L

A

K

G

A

M

-

L

-

E

E

P

T

K

T

L

G

A

D

E

P

S

A

V

H

K

R

P

S

E

P

D

H

A

P

E

D

A

A

C

A

L

F

N

L

L

F

P

A

G

D

Q

R

M

A

V

I

L

A

F

L

D

D

H

H

L

-

R

Q

H

E

S

G

C

C

Y

Y

L

L

-

L

S

A

L

L

D

D

K

R

G

R

S

G

V

H

P

D

R

D

P

G

V

A

-

R

-

A

-

W

-

L

-

R

-

E

A

T

A

A

E

E

W

T

G

L

T

T

D

G

L

L

A

A

-

V

-

R

A

A

P

P

E

A

A

E

S

P

E

T

P

P

Q

A

M

M

Y

V

-

F

-

G

-

A

-

G

-

F

-

G

-

P

-

L

-

A

-

R

-

A

-

R

P

H

E

D

R

K

D

D

D

A

Y

Y

L

L

R

K

A

R

V

I

E

D

K

E

T

C

K

L

E

K

L

E

I

I

A

R

D

N

G

G

E

E

C

S

I

Y

Q

E

A

I

V

C

L

L

S

T

T

N

R

M

F

V

T

T

V

A

P

P

L

T

P

E

D

A

D

T

P

A

F

L

R

P

I

L

Y

Y

R

S

R

A

L

L

R

R

Q

A

A

I

N

S

P

P

S

V

P
|
P

F
x
Y

M
x
G

F

A

Y

L

M

L

K

E

L

F

P

P

G

-

C

-

D

E

G

L

T

S

L

V

L

L

G

S

S

A

S

S

P

P

E

E

M

R

M

F

V

L

R

T

C

I

-

G

E

A

E

D

G

G

R

G

L

V

E

E

V

S

R

K

P

P

I

I

A

K

G

G

T

T

R

R

P

P

R

R

G

G

N

T

E

A

S

E

E

E

D

D

L

E

A

R

L

L

A

R

D

A

E

D

L

L

L

A

A

G

D

R

P

E

K

K

E

D

R

R

A

A

E
|
E

H

N

V

L

M

M

L

I

V

V

D

D

L

L

G

V

R

R

N

N

D

D

L

L

G

N

R

S

I

V

S

C

N

A

P

I

G

G

T

S

V

V

E

H

V

V

K

P

R

R

F

L

M

F

N

E

V

V

E

E

R

T

F

Y

S

A

H

P

V

V

M

H

H

Q

L

L

T

V

S

S

Y

T

V

I

E

R

G

G

E

R

L

L

Q

R

D

P

G

G

L

T

D

S

A

T

V

A

D

A

V

C

L

V

R

R

A

A

T

A

F

F

P

P

A

G

G

G

T

S

L

M

S

T

G

G

A

A

P

P

K

K

V

K

R

R

A

T

M

M

E

E

I

I

S

D

E

R

M

L

E

E

P

E

Q

G

E

P

R

R

G

G

P

V

Y

Y

G

S

G

G

C

A

I

L

G
|
G

F

W

L

F

G

A

L

L

D

-

S

-

D

-

S

S

V

G

S

A

L

A

D

D

T

L

G

S

I

I

T

V

I

I

R

R

S

T

L

I

W

V

V

L

R

A

N

D

G

G

Q

Q

C

A

H

E

W

F

Q

G

A

V

G

G

A

G

G

A

I

I

V

V

H

S

D

L

S

S

V

D

P

Q

E

E

K

E

E
|
E

W

F

E

T

E
|
E

C

T

R

V

N

V

K

K

A

A

R

R

V

A

L

M

M

V

E

T

V

A

I

L

N

D

G

G

binding magnesium ion: E521 (= E310), E655 (= E447), E658 (= E450)
binding tryptophan: P458 (= P246), Y459 (≠ F247), G460 (≠ M248), G614 (= G403)

Sites not aligning to the query:

binding magnesium ion: 175, 177
binding tryptophan: 231, 232, 233, 241, 243

8hx9A Crystal structure of 4-amino-4-deoxychorismate synthase from streptomyces venezuelae with chorismate (see paper)
32% identity, 74% coverage: 114:463/474 of query aligns to 266:632/632 of 8hx9A

query
sites
8hx9A

G

G

L

Y

Y

V

G

G

Y

Y

L

L

G

G

Y

Y

G

E

I

L

A

K

G

A

M

-

L

-

E

E

P

T

K

T

L

G

A

D

E

P

S

A

V

H

K

R

P

S

E

P

D

H

A

P

E

D

A

A

C

A

L

F

N

L

L

F

P

A

G

D

Q

R

M

A

V

I

L

A

F

L

D

D

H

H

L

-

R

Q

H

E

S

G

C

C

Y

Y

L

L

-

L

S

A

L

L

D

D

K

R

G

R

S

G

V

H

P

D

R

D

P

G

V

A

-

R

-

A

-

W

-

L

-

R

-

E

A

T

A

A

E

E

W

T

G

L

T

T

D

G

L

L

A

A

A

V

P

R

E

A

A

P

S

P

E

T

P

P

Q

A

M

M

Y

V

-

F

-

G

-

I

P

P

E

E

-

A

-

G

-

F

-

G

-

P

-

L

-

A

-

R

-

A

-

R

-

H

-

D

R

K

D

D

D

A

Y

Y

L

L

R

K

A

R

V

I

E

D

K

E

T

C

K

L

E

K

L

E

I

I

A

R

D

N

G

G

E

E

C

S

I

Y

Q

E

A

I

V

C

L

L

S

T

T

N

R

M

F

V

T

T

V

A

P

P

L

T

P

E

D

A

D

T

P

A

F

L

R

P

I

L

Y

Y

R

S

R

A

L

L

R

R

Q

A

A

I

N

S

P

P

S

V

P
|
P

F
x
Y

M
x
G

F

A

Y

L

M

L

K

E

L

F

P

P

G

-

C

-

D

E

G

L

T

S

L

V

L

L

G

S

S

A

S

S

P

P

E

E

M

R

M

F

V

L

R

T

C

I

-

G

E

A

E

D

G

G

R

G

L

V

E

E

V

S

R

K

P

P

I
|
I

A
x
K

G
|
G

T
|
T

R

R

P

P

R

R

G

G

N

T

E

A

S

E

E

E

D

D

L

E

A

R

L

L

A

R

D

A

E

D

L

L

L

A

A

G

D

R

P

E

K

K

E

D

R

R

A

A

E

E

H

N

V

L

M

M

L

I

V

V

D

D

L

L

G

V

R

R

N

N

D

D

L

L

G

N

R

S

I

V

S

C

N

A

P

I

G

G

T

S

V

V

E

H

V

V

K

P

R

R

F

L

M

F

N

E

V

V

E

E

R

T

F

Y

S

A

H

P

V

V

M

H

H

Q

L

L

T

V

S

S

Y

T

V

I

E

R

G

G

E

R

L

L

Q

R

D

P

G

G

L

T

D

S

A

T

V

A

D

A

V

C

L

V

R

R

A

A

T

A

F

F

P

P

A

G

G

G

T

S

L
x
M

S

T

G

G

A

A

P

P

K

K

V

K

R

R

A

T

M

M

E

E

I

I

S

D

E

R

M

L

E

E

P

E

Q

G

E

P

R

R

G

G

P

V

Y
|
Y

G

S

G

G

C

A

I
x
L

G
|
G

F

W

L

F

G

A

L

L

D

-

S

-

D

-

S

S

V

G

S

A

L

A

D

D

T

L

G

S

I

I

T

V

I

I

R
|
R

S

T

L

I

W

V

V

L

R

A

N

D

G

G

Q

Q

C

A

H

E

W

F

Q

G

A
x
V

G
|
G

A
x
G

G
x
A

I

I

V

V

H

S

D

L

S

S

V

D

P

Q

E

E

K

E

E

E

W

F

E

T

E
|
E

C

T

R

V

N

V

K
|
K

A

A

R

R

V

A

L

M

M

V

E

T

V

A

I

L

N

D

binding (3R,4R)-3-[(1-carboxyethenyl)oxy]-4-hydroxycyclohexa-1,5-diene-1-carboxylic acid: I453 (= I281), K454 (≠ A282), G455 (= G283), T456 (= T284), M547 (≠ L375), Y570 (= Y398), R590 (= R421), V603 (≠ A434), G604 (= G435), G605 (≠ A436), A606 (≠ G437), E619 (= E450), K623 (= K454)
binding tryptophan: P419 (= P246), Y420 (≠ F247), G421 (≠ M248), L574 (≠ I402), G575 (= G403)

Sites not aligning to the query:

binding magnesium ion: 134, 135
binding tryptophan: 189, 190, 191, 199, 201

2fn1A Crystal structures of yersinia enterocolitica salicylate synthase (irp9) in complex with the reaction products salicylate and pyruvate (see paper)
34% identity, 54% coverage: 197:454/474 of query aligns to 147:398/408 of 2fn1A

query
sites
2fn1A

D

E

D

A

Y

Y

L

K

R

Q

A

Q

V

V

E

A

K

R

T

A

K

V

E

A

L

E

I

I

A

R

D

R

G

G

E

E

C

Y

I

V

Q
x
K

A

V

V

I

L

V

S

S

T

R

R

A

F

I

T

P

V

L

P

P

L

S

P

R

D

I

D

D

P

M

F

P

R

A

I

T

Y

L

R

L

R

Y

L

G

R

R

Q

Q

A

A

N

N

P

T

S

P

P

V

F

R

M

S

F

F

Y

M

M

F

K

R

L

Q

P

E

G

G

C

R

D

E

G

A

T

-

L

-

L

L

G

G

S

F

S

S

P

P

E
|
E

M

L

M

V

V

M

R

S

C

V

E

T

E

G

G

N

R

K

L

V

E

V

V

T

R

E

P

P

I

L

A
|
A

G

G

T

T

R

R

P

D

R

R

G

M

G

G

N

N

E

P

S

E

E

H

D

N

L

K

A

A

L

K

A

E

D

A

E

E

L

L

A

H

D

D

P

S

K

K

E

E

R

V

A

L

E
|
E

H

H

V

I

M

L

L

S

V

V

D

K

L

E

G

A

R

I

N

A

D

E

L

L

G

E

R

A

I

V

S

C

N

L

P

P

G

G

T

S

V

V

E

V

V

V

K

E

R

D

F

L

M

M

N

S

V

V

E

R

R

Q

F

R

S

G

H

S

V

V

M

Q

H
|
H

L

L

T

G

S

S

Y

G

V

V

E

S

G

G

E

Q

L

L

Q

A

D

E

G

N

L

K

D

D

A

A

V

W

D

D

V

A

L

F

R

T

A

V

T

L

F

F

P

P

A

S

G

I

T
|
T

L

A

S

S

G

G

A

I

P

P

K

K

V

N

R

A

A

A

M

L

E

N

I

A

I

I

S

M

E

Q

M

I

E

E

P

K

Q

T

E

P

R

R

G

E

P

L

Y
|
Y

G

S

G

G

C

A

I

I

G

L

F

L

L

L

G

-

L

-

D

-

S

-

D

D

S

D

V

T

S

R

L

F

D

D

T

A

G

A

I

L

T

V

I
x
L

R
|
R

S

S

L

V

W

F

V

Q

R

D

N

S

G

Q

Q

R

C

C

H

W

W

I

Q

Q

A
|
A

G
|
G

A

A

G
|
G

I

I

V

I

H

A

D

Q

S

S

V

T

P

P

E

E

K

R

E

E

W

L

E

T

E
|
E

C

T

R

R

N

E

K
|
K

active site: K167 (≠ Q217), E214 (= E266), A230 (= A282), E258 (= E310), H295 (= H347), T322 (= T374), Y346 (= Y398), R365 (= R421), G381 (= G437), E394 (= E450), K398 (= K454)
binding magnesium ion: E258 (= E310), E394 (= E450)
binding pyruvic acid: Y346 (= Y398), L364 (≠ I420), R365 (= R421), A378 (= A434), G379 (= G435), K398 (= K454)

Query Sequence

>WP_018123679.1 NCBI__GCF_000375485.1:WP_018123679.1
MKPIQLKQYGKWLPADVQTPISLYLGLVGDAPGILLESAEVDGRLGRYTLIAWDFRLSLR
PENGKLAVEATDERLKPLEKFSGMNFLDGLRRVLRAVAVEQETDDGPLPALTRGLYGYLG
YGIAGMLEPKLAESVKPEDAEACLNLPGQMVLFDHLRHSCCYLSLDKGSVPRPVAAEWGT
DLAAPEASEPQMYPERDDYLRAVEKTKELIADGECIQAVLSTRFTVPLPDDPFRIYRRLR
QANPSPFMFYMKLPGCDGTLLGSSPEMMVRCEEGRLEVRPIAGTRPRGGNESEDLALADE
LLADPKERAEHVMLVDLGRNDLGRISNPGTVEVKRFMNVERFSHVMHLTSYVEGELQDGL
DAVDVLRATFPAGTLSGAPKVRAMEIISEMEPQERGPYGGCIGFLGLDSDSVSLDTGITI
RSLWVRNGQCHWQAGAGIVHDSVPEKEWEECRNKARVLMEVINGRGGTDVFAHR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory