SitesBLAST
Comparing WP_018123799.1 NCBI__GCF_000375485.1:WP_018123799.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
48% identity, 100% coverage: 1:467/467 of query aligns to 1:463/471 of P04805
- C98 (= C98) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C100) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C125) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ E127) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ G129) mutation to Q: No change in activity or in zinc content.
- H131 (≠ E131) mutation to Q: No change in activity or in zinc content.
- H132 (≠ A132) mutation to Q: No change in activity or in zinc content.
- C138 (vs. gap) mutation to S: No change in activity or in zinc content.
- S239 (= S237) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
48% identity, 100% coverage: 1:467/467 of query aligns to 1:463/468 of 8i9iA
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
46% identity, 82% coverage: 3:383/467 of query aligns to 3:369/380 of 4g6zA
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
36% identity, 100% coverage: 2:467/467 of query aligns to 3:468/488 of 8vc5A
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
39% identity, 99% coverage: 6:467/467 of query aligns to 106:561/564 of 3al0C
- active site: S110 (= S10), K335 (= K238)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R6), A108 (= A8), P109 (= P9), G118 (= G18), T122 (= T22), E142 (= E42), Y276 (= Y179), R294 (= R197), G295 (= G198), D297 (= D200), H298 (= H201), L324 (≠ M227), I325 (= I228), L333 (= L236)
- binding : T144 (= T44), D145 (= D45), R148 (= R48), Y208 (≠ C100), P213 (≠ A107), K252 (= K155), M255 (≠ I158), I266 (= I169), K269 (≠ R172), S270 (= S173), Y276 (= Y179), D297 (= D200), H298 (= H201), L299 (≠ V202), S300 (≠ N203), N301 (= N204), K304 (≠ R207), R330 (≠ K233), P332 (≠ K235), G363 (= G266), W364 (= W267), R365 (≠ S268), E370 (= E273), S387 (≠ G290), K389 (≠ S292), V391 (≠ S294), I392 (≠ V295), K397 (= K300), W400 (= W303), R407 (≠ K310), E446 (≠ P351), K447 (≠ R352), Q453 (≠ E358), I457 (≠ Q362), R509 (≠ K415), K520 (= K426), Q524 (= Q430), R527 (= R433), V535 (≠ K441), T536 (≠ S442), G538 (≠ S444), L539 (≠ I445)
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
35% identity, 98% coverage: 6:464/467 of query aligns to 5:478/485 of 4griB
- active site: S9 (= S10), K253 (= K238)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (= E42), Y194 (= Y179), R212 (= R197), W216 (≠ H201)
- binding zinc ion: C105 (= C98), C107 (= C100), Y128 (= Y121), C132 (= C125)
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
39% identity, 99% coverage: 3:465/467 of query aligns to 2:467/468 of 1g59A
- binding : D44 (= D45), R45 (= R46), A46 (≠ E47), R47 (= R48), P109 (≠ K102), V145 (= V137), R163 (≠ K155), V166 (≠ I158), E172 (= E164), V177 (≠ I169), K180 (≠ R172), S181 (= S173), D182 (= D174), E207 (≠ D199), E208 (≠ D200), R237 (≠ L229), K241 (= K233), T242 (≠ K234), K243 (= K235), M273 (≠ L265), G274 (= G266), E282 (= E273), S299 (≠ G290), L300 (≠ N291), P303 (≠ S294), V304 (= V295), K309 (= K300), W312 (= W303), R319 (≠ K310), P357 (= P351), R358 (= R352), R417 (≠ K415), K426 (= K424), L427 (≠ F425), Q432 (= Q430), R435 (= R433), L442 (≠ T440), E443 (≠ K441), T444 (≠ S442), P445 (= P443), G446 (≠ S444), L447 (≠ I445), F448 (≠ Y446)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
39% identity, 99% coverage: 3:465/467 of query aligns to 2:467/468 of 2cv2A
- active site: K246 (= K238)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R6), A7 (= A8), S9 (= S10), G17 (= G18), I21 (≠ T22), E41 (= E42), Y187 (= Y179), R205 (= R197), A206 (≠ G198), E208 (≠ D200), W209 (≠ H201), L235 (≠ M227), L236 (≠ I228)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V137), R163 (≠ K155), Y168 (≠ V160), E172 (= E164), V177 (≠ I169), K180 (≠ R172), S181 (= S173), Y187 (= Y179), E207 (≠ D199), E208 (≠ D200), W209 (≠ H201), V211 (≠ N203), R237 (≠ L229), K241 (= K233), L272 (≠ R264), M273 (≠ L265), G274 (= G266), E282 (= E273), S299 (≠ G290), P303 (≠ S294), V304 (= V295), K309 (= K300), W312 (= W303), R319 (≠ K310), P357 (= P351), R358 (= R352), R417 (≠ K415), Q432 (= Q430), R435 (= R433), L442 (≠ T440), E443 (≠ K441), T444 (≠ S442), G446 (≠ S444), L447 (≠ I445), F448 (≠ Y446)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
39% identity, 99% coverage: 3:465/467 of query aligns to 2:467/468 of 2cv1A
- active site: K246 (= K238)
- binding adenosine-5'-triphosphate: P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ G19), I21 (≠ T22), R47 (= R48), A206 (≠ G198), W209 (≠ H201), L235 (≠ M227), L236 (≠ I228)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R6), A7 (= A8), E41 (= E42), Y187 (= Y179), R205 (= R197), W209 (≠ H201)
- binding : S9 (= S10), E41 (= E42), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V137), R163 (≠ K155), V166 (≠ I158), E172 (= E164), V177 (≠ I169), K180 (≠ R172), S181 (= S173), Y187 (= Y179), E207 (≠ D199), E208 (≠ D200), W209 (≠ H201), V211 (≠ N203), R237 (≠ L229), K241 (= K233), K243 (= K235), M273 (≠ L265), G274 (= G266), S276 (= S268), E282 (= E273), S299 (≠ G290), P303 (≠ S294), V304 (= V295), K309 (= K300), W312 (= W303), R319 (≠ K310), P357 (= P351), R358 (= R352), R417 (≠ K415), L427 (≠ F425), Q432 (= Q430), R435 (= R433), L442 (≠ T440), E443 (≠ K441), T444 (≠ S442), G446 (≠ S444), L447 (≠ I445), F448 (≠ Y446)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
39% identity, 99% coverage: 3:465/467 of query aligns to 2:467/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
39% identity, 99% coverage: 3:465/467 of query aligns to 2:467/468 of 1n78A
- active site: K246 (= K238)
- binding glutamol-amp: R5 (= R6), A7 (= A8), P8 (= P9), S9 (= S10), G17 (= G18), T18 (≠ G19), I21 (≠ T22), E41 (= E42), Y187 (= Y179), N191 (≠ V183), R205 (= R197), A206 (≠ G198), E208 (≠ D200), W209 (≠ H201), L235 (≠ M227), L236 (≠ I228)
- binding : S9 (= S10), T43 (= T44), D44 (= D45), R47 (= R48), V145 (= V137), R163 (≠ K155), V166 (≠ I158), Y168 (≠ V160), E172 (= E164), V177 (≠ I169), K180 (≠ R172), S181 (= S173), Y187 (= Y179), E207 (≠ D199), E208 (≠ D200), W209 (≠ H201), L210 (≠ V202), V211 (≠ N203), R237 (≠ L229), K241 (= K233), M273 (≠ L265), G274 (= G266), E282 (= E273), R297 (≠ N288), P303 (≠ S294), V304 (= V295), K309 (= K300), W312 (= W303), R319 (≠ K310), P357 (= P351), R358 (= R352), R417 (≠ K415), L427 (≠ F425), Q432 (= Q430), R435 (= R433), L442 (≠ T440), E443 (≠ K441), T444 (≠ S442), G446 (≠ S444), L447 (≠ I445), F448 (≠ Y446)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
39% identity, 99% coverage: 3:465/467 of query aligns to 2:467/468 of 1j09A
- active site: K246 (= K238)
- binding adenosine-5'-triphosphate: H15 (= H16), E208 (≠ D200), L235 (≠ M227), L236 (≠ I228), K243 (= K235), I244 (≠ L236), S245 (= S237), K246 (= K238), R247 (= R239)
- binding glutamic acid: R5 (= R6), A7 (= A8), S9 (= S10), E41 (= E42), Y187 (= Y179), N191 (≠ V183), R205 (= R197), W209 (≠ H201)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
39% identity, 99% coverage: 3:465/467 of query aligns to 2:467/468 of P27000
- R358 (= R352) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
36% identity, 97% coverage: 1:452/467 of query aligns to 1:464/485 of Q8DLI5
- R6 (= R6) binding L-glutamate
- Y192 (= Y179) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
36% identity, 97% coverage: 2:452/467 of query aligns to 1:463/484 of 2cfoA
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
32% identity, 99% coverage: 6:466/467 of query aligns to 6:499/502 of 6brlA
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
41% identity, 51% coverage: 4:243/467 of query aligns to 5:234/290 of 4a91A
- active site: S11 (= S10), K229 (= K238)
- binding glutamic acid: R7 (= R6), A9 (= A8), S11 (= S10), E43 (= E42), Y170 (= Y179), R188 (= R197), L192 (≠ H201)
- binding zinc ion: C99 (= C98), C101 (= C100), Y113 (= Y121), C117 (= C125)
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
41% identity, 51% coverage: 4:242/467 of query aligns to 17:245/308 of P27305
- E55 (= E42) binding L-glutamate
- Y182 (= Y179) binding L-glutamate
- R200 (= R197) binding L-glutamate
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
36% identity, 48% coverage: 3:224/467 of query aligns to 11:228/455 of 3aiiA
Sites not aligning to the query:
O13775 Probable glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 51% coverage: 3:238/467 of query aligns to 207:449/716 of O13775
Sites not aligning to the query:
- 190 modified: Phosphoserine
Query Sequence
>WP_018123799.1 NCBI__GCF_000375485.1:WP_018123799.1
MTIVSRFAPSPTGFLHIGGARTALFSWLLARHSGGKFLLRVEDTDRERSTQEAIDAILDA
MKWLGLEHDGEITYQSERADRHNEVIDQLIEAGHAYYCDCTKEQVDAMREKARAEGRKPK
YDGSCREKGLEAGPDRVVRLKAPLDGVTAYDDLVKGPIAVNNEEMDDMILRRSDGSPTYN
LAVVVDDHDMGVNHVLRGDDHVNNTPRQILIYKAMGWEVPTFGHVPMILGPDKKKLSKRH
GALSVMEYEKLGYLPEAACNYLARLGWSHGDQEIFSRQEMIDLFDTANLGNSPSVFDLKK
FEWVNAHWIKEADPERLTDMVMDFIGRELGEEAAASAPRDKVLALVPHLQPRAKAIPEMV
EQARPFIVDAASLPYDEKAVTKFITEEAKPLLQDVLNRLEADETFTHDSLEAVCKGVLEE
RELKFKVLAQPLRVAVTGTTKSPSIYDTMLVLGREETLARIRRALDM
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory