SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_018125315.1 NCBI__GCF_000375485.1:WP_018125315.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

1um0A Crystal structure of chorismate synthase complexed with fmn (see paper)
45% identity, 97% coverage: 4:345/354 of query aligns to 2:348/365 of 1um0A

query
sites
1um0A

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binding flavin mononucleotide: H104 (= H106), R123 (= R126), S125 (= S128), N241 (≠ G238), G242 (≠ A239), L280 (= L277), G281 (= G278), K296 (= K293), T298 (≠ I295), P299 (= P296), S300 (= S297), I327 (≠ A324), R330 (= R327)

P56122 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
45% identity, 97% coverage: 4:345/354 of query aligns to 2:348/365 of P56122

query
sites
P56122

N

N

T

T

F

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RSS 123:125 (= RSS 126:128) binding FMN
KPTPS 296:300 (≠ KPIPS 293:297) binding FMN

Q12640 Chorismate synthase aro-2; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 1.5.1.38; EC 4.2.3.5 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 3 papers)
37% identity, 97% coverage: 4:345/354 of query aligns to 2:391/432 of Q12640

query
sites
Q12640

N

S

T

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Y

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S16 (= S18) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 70-fold.
H17 (= H19) active site; mutation to A: Reduces the chorismate synthase activity 20-fold.
H106 (= H106) active site; mutation to A: Reduces the chorismate synthase activity 10-fold.
S127 (= S128) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 6-fold.
D367 (= D321) active site; mutation D->A,N: Does not affect substrate binding but stringly reduces the chorismate synthase activity.

P9WPY1 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
34% identity, 95% coverage: 9:345/354 of query aligns to 1:367/401 of P9WPY1

query
sites
P9WPY1

L

M

F

L

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W

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A

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x
R

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A

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P

G

V

G

L

G

E

R

R

S

A

S

S

G

A

R

R

E

E

T

T

V

A

A

A

R

R

V

V

A

A

G

A

G

G

A

T

V

V

A

A

L

R

E

A

F

F

L

L

R

R

S

Q

E

A

-

L

G

G

I

V

S

E

V

V

F

L

A

S

Y

H

T

V

A

I

E

S

I

I

G

G

-

A

-

S

-

A

-

P

-

Y

-

E

G

G

I

P

S

P

A

P

D

R

P

A

V

E

D

D

M

L

E

P

N

A

A

I

Q

D

N

A

N

S

P

P

F

V

F

R

S

A

P

Y

D

D

G

K

E

A

V

A

V

E

E

A

K

D

W

M

E

I

E

A

R

Q

I

I

R

E

E

A

V

A

K

K

G

K

L

D

G

G

D

D

T

T

L

L

G

G

G

G

V

V

V

V

E

E

V

A

R

V

I

A

A

L

G

G

L

L

A

P

P

V

G

G

I

L

G

G

E

S

P

F

V

T

F

S

-

G

-

D

D

H

K

R

L

L

D

D

A

S

R

Q

L

L

A

A

Y

A

A

A

L

V

M

M

G

G

V

I

G
x
Q

A
|
A

V

I

K

K

A

G

V

V

E

E

I

I

G

G

S

D

G

G

C

F

D

Q

A

T

A

A

R

R

K

R

L

R

G

G

S

S

E

R

N

A

N

H

D

D

Y

E

M

M

T

Y

T

P

A

G

G

P

-

D

-

G

-

V

-

V

F

R

S

S

S

T

N

N

N

R

A

A

G

G

G

G

I

L

L

E

G

G

G

G

I

M

S

T

S

N

G

G

Q

Q

D

P

V

L

V

R

A

V

R

R

A

A

F

A

V

M

K
|
K

P
|
P

I
|
I

P
x
S

S
x
T

I

V

A

P

Q

R

E

A

Q

L

H

A

C

T

M

V

D

D

V

L

E

A

G

T

K

G

R

D

V

E

C

A

M

V

R

A

I

I

G

H

G

Q

R
|
R

H

S

D

D

I

V

C

C

A

A

I

V

P

P

R

A

I

A

N

G

P

V

V

V

L

V

K

E

A

T

M

M

T

V

A

A

L

L

T

V

L

L

A

A

D

R

L

A

I

A

L

L

R40 (= R48) binding NADP(+)
R46 (≠ A54) binding NADP(+)
QA 256:257 (≠ GA 238:239) binding FMN
KPIST 315:319 (≠ KPIPS 293:297) binding FMN
R341 (= R319) binding FMN

2qhfA Mycobacterium tuberculosis chorismate synthase in complex with nca
34% identity, 95% coverage: 9:345/354 of query aligns to 1:367/392 of 2qhfA

query
sites
2qhfA

L

M

F

L

T

R

L

W

T

I

T

T

F

A

G

G

E

E

S

S

H

H

G

G

P

R

G

A

L

L

G

V

G

A

V

V

V

V

D

E

G

G

C

M

P

V

A

A

G

G

I

V

P

H

M

V

S

T

E

S

E

A

I

D

V

I

Q

A

Q

D

E

Q

L

L

D

A

R

R

R

R

K

R

P

L

G

G

Q

Y

G

G

A
x
R

A

G

S

A

T

R

A

M

R

T

K

F

E

E

S

R

D

D

T

A

V

V

R

T

I

V

Y

L

S

S

G

G

V

I

F

R

E

H

G

G

Q

S

T

T

T

L

G

G

T

G

P

P

I

I

G

A

F

I

V

E

V

I

E

G

N

N

E

T

D

E

Q

W

R

P

S

K

R

W

-

E

-

T

-

V

-

M

-

A

-

A

-

D

-

P

-

V

D

D

Y

P

S

A

R

E

V

L

K

A

D

D

V

V

F

A

-

R

-

N

-

A

-

P

-

L

-

T

-

R

-

P

R

R

P

P

G

G

H

H

A

A

D

D

F

Y

T

A

Y

G

H

M

S

L

K

K

F

Y

G

G

G

F

V

D

R

D

D

A

Y

R

R

P

G

V

G

L

G

E

R

R

S

A

S

S

G
x
A

R

R

E

E

T

T

V

A

A

A

R

R

V

V

A

A

G

A

G

G

A

T

V

V

A

A

L

R

E

A

F

F

L

L

R

R

S

Q

E

A

-

L

G

G

I

V

S

E

V

V

F

L

A

S

Y

H

T

V

A

I

E

S

I

I

G

G

-

A

-

S

-

A

-

P

-

Y

-

E

G

G

I

P

S

P

A

P

D

R

P

A

V

E

D

D

M

L

E

P

N

A

A

I

Q

D

N

A

N

S

P

P

F

V

F

R

S

A

P

Y

D

D

G

K

E

A

V

A

V

E

E

A

K

D

W

M

E

I

E

A

R

Q

I

I

R

E

E

A

V

A

K

K

G

K

L

D

G

G

D

D

T

T

L

L

G

G

G

G

V

V

V

V

E

E

V

A

R

V

I

A

A

L

G

G

L

L

A

P

P

V

G

G

I

L

G

G

E

S

P

F

V

T

F

S

-

G

-

D

D

H

K

R

L

L

D

D

A

S

R

Q

L

L

A

A

Y

A

A

A

L

V

M

M

G

G

V
x
I

G
x
Q

A

A

V

I

K

K

A

G

V

V

E

E

I

I

G

G

S

D

G

G

C

F

D

Q

A

T

A

A

R

R

K

R

L

R

G

G

S

S

E

R

N

A

N

H

D

D

Y

E

M

M

T

Y

T

P

A

G

G

P

-

D

-

G

-

V

-

V

F

R

S

S

S

T

N

N

N

R

A

A

G

G

G

G

I

L

L

E

G

G

G

G

I

M

S

T

S

N

G

G

Q

Q

D

P

V

L

V

R

A

V

R

R

A

A

F

A

V
x
M

K

K

P

P

I
|
I

P

S

S

T

I

V

A

P

Q

R

E

A

Q

L

H

A

C

T

M

V

D

D

V

L

E

A

G

T

K

G

R

D

V

E

C

A

M

V

R

A

I

I

G

H

G

Q

R
|
R

H

S

D

D

I

V

C

C

A

A

I

V

P

P

R

A

I

A

N

G

P

V

V

V

L

V

K

E

A

T

M

M

T

V

A

A

L

L

T

V

L

L

A

A

D

R

L

A

I

A

L

L

binding nicotinamide: R46 (≠ A54), A138 (≠ G129), I255 (≠ V237), Q256 (≠ G238), M314 (≠ V292), I317 (= I295), R341 (= R319)

2o12A Mycobacterium tuberculosis chorismate synthase in complex with fmn
34% identity, 95% coverage: 9:345/354 of query aligns to 1:361/386 of 2o12A

query
sites
2o12A

L

M

F

L

T

R

L

W

T

I

T

T

F

A

G

G

E

E

S

S

H

H

G

G

P

R

G

A

L

L

G

V

G

A

V

V

V

V

D

E

G

G

C

M

P

V

A

A

G

G

I

V

P

H

M

V

S

T

E

S

E

A

I

D

V

I

Q

A

Q

D

E

Q

L

L

D

A

R

R

R

R

K

R

P

L

G

G

Q

Y

G

G

A

-

A

-

S

-

T

-

A

-

R

-

K

R

E

E

S

R

D

D

T

A

V

V

R

T

I

V

Y

L

S

S

G

G

V

I

F

R

E

H

G

G

Q

S

T

T

T

L

G

G

T

G

P

P

I

I

G

A

F

I

V

E

V

I

E

G

N

N

E

T

D

E

Q

W

R

P

S

K

R

W

-

E

-

T

-

V

-

M

-

A

-

A

-

D

-

P

-

V

D

D

Y

P

S

A

R

E

V

L

K

A

D

D

V

V

F

A

R

R

-

N

-

A

-

P

-

L

-

T

-

R

-

P

-

R

P

P

G

G

H
|
H

A

A

D

D

F

Y

T

A

Y

G

H

M

S

L

K

K

F

Y

G

G

G

F

V

D

R

D

D

A

Y

R

R

P

G

V

G

L

G

E

R

R

S

A

S

S

G
x
A

R

R

E

E

T

T

V

A

A

A

R

R

V

V

A

A

G

A

G

G

A

T

V

V

A

A

L

R

E

A

F

F

L

L

R

R

S

Q

E

A

-

L

G

G

I

V

S

E

V

V

F

L

A

S

Y

H

T

V

A

I

E

S

I

I

G

G

-

A

-

S

-

A

-

P

-

Y

-

E

G

G

I

P

S

P

A

P

D

R

P

A

V

E

D

D

M

L

E

P

N

A

A

I

Q

D

N

A

N

S

P

P

F

V

F

R

S

A

P

Y

D

D

G

K

E

A

V

A

V

E

E

A

K

D

W

M

E

I

E

A

R

Q

I

I

R

E

E

A

V

A

K

K

G

K

L

D

G

G

D

D

T

T

L

L

G

G

G

G

V

V

V

V

E

E

V

A

R

V

I

A

A

L

G

G

L

L

A

P

P

V

G

G

I

L

G

G

E

S

P

F

V

T

F

S

-

G

-

D

D

H

K

R

L

L

D

D

A

S

R

Q

L

L

A

A

Y

A

A

A

L

V

M

M

G

G

V

I

G
x
Q

A
|
A

V

I

K

K

A

G

V

V

E

E

I

I

G

G

S

D

G

G

C

F

D

Q

A

T

A

A

R

R

K

R

L

R

G

G

S

S

E

R

N

A

N

H

D

D

Y

E

M

M

T

Y

T

P

A

G

G

P

-

D

-

G

-

V

-

V

F

R

S

S

S

T

N

N

N

R

A

A

G

G

G

G

I

L

L

E

G

G

G

G

I

M

S

T

S

N

G

G

Q

Q

D

P

V

L

V

R

A

V

R

R

A

A

F

A

V
x
M

K
|
K

P

P

I
|
I

P

S

S
x
T

I

V

A

P

Q

R

E

A

Q

L

H

A

C

T

M

V

D

D

V

L

E

A

G

T

K

G

R

D

V

E

C

A

M

V

R

A

I

I

G

H

G

Q

R
|
R

H

S

D

D

I

V

C

C

A

A

I

V

P

P

R

A

I

A

N

G

P

V

V

V

L

V

K

E

A

T

M

M

T

V

A

A

L

L

T

V

L

L

A

A

D

R

L

A

I

A

L

L

binding flavin mononucleotide: H109 (= H106), A132 (≠ G129), Q250 (≠ G238), A251 (= A239), M308 (≠ V292), K309 (= K293), I311 (= I295), T313 (≠ S297), R335 (= R319)

1qxoA Crystal structure of chorismate synthase complexed with oxidized fmn and epsp (see paper)
35% identity, 94% coverage: 14:345/354 of query aligns to 5:363/388 of 1qxoA

query
sites
1qxoA

T

T

F

A

G

G

E

E

S

S

H

H

G

G

P

P

G

R

L

L

G

T

G

A

V

I

V

I

D

E

G

G

C

I

P

P

A

A

G

G

I

L

P

P

M

L

S

T

E

A

E

E

I

D

V

I

Q

N

Q

E

E

D

L

L

D

R

R

R

R
|
R

K

Q

P

G

G

G

Q

Y

G

G

A
x
R

A

G

S

G

T
x
R

A
x
M

R

K

K

I

E

E

S

N

D

D

T

Q

V

V

R

V

I

F

Y

T

S

S

G

G

V

V

F

R

E

H

G

G

Q

K

T

T

T

T

G

G

T

A

P

P

I

I

G

T

F

M

V

D

V

V

E

I

N

N

E

K

D

D

-

H

Q

Q

R

K

S

W

R

L

D

D

Y

I

S

M

R

S

V

A

K

E

D

D

V

I

F

E

-

D

-

R

-

L

-

K

-

S

-

K

-

R

-

K

-

I

-

T

-

H

-

P

R

R

P

P

G

G

H
|
H

A

A

D

D

F

L

T

V

Y

-

H

-

S

-

K

-

F

-

G

G

G

G

V

I

R

K

-

Y

-

R

-

F

-

D

D

D

Y

L

R

R

G

N

G

S

-

L

G

E

R

R

S

S

S

S

G
x
A

R
|
R

E

E

T

T

V

T

A

M

R

R

V

V

A

A

G

V

G

G

A

A

V

V

A

A

L

K

E

R

F

L

L

L

R

A

S

E

E

L

G

D

I

M

S

E

V

I

F

A

A

N

Y

H

T

V

A

V

E

V

I

F

G

G

G

G

I

K

S

E

A

I

D

D

-

V

P

P

V

E

D

N

M

L

-

T

-

V

-

A

-

E

-

I

-

K

E

Q

N

R

A

A

Q

A

N

Q

N

S

P

E

F

V

F

S

S

I

P

V

D

N

G

Q

E

E

V

R

V

E

E

Q

K

E

W

I

E

K

E

D

R

Y

I

I

R

D

E

Q

V

I

K

K

G

R

L

D

G

G

D

D

T

T

L

I

G

G

G

G

V

V

V

V

E

E

V

T

R

V

I

V

A

G

G

G

L

V

A

P

P

V

G

G

I

L

G

G

E

S

P

Y

V

V

-

Q

F

W

D

D

-

R

K

K

L

L

D

D

A

A

R

R

L

L

A

A

Y

Q

A

A

L

V

M

V

G

S

V

I

G
x
N

A
|
A

V

F

K

K

A

G

V

V

E

E

I

F

G

G

S

L

G

G

C

F

D

E

A

A

A

G

R

Y

K

R

L

K

G

G

S

S

E

Q

N

V

N

M

D

D

-

E

-

I

-

L

Y

W

M

S

T

K

T

E

A

D

G

G

F

Y

S

T

-

R

-

R

S

T

N

N

N

N

A

L

G

G

G

G

I

F

L

E

G

G

G

G

I

M

S

T

S

N

G

G

Q

Q

D

P

V

I

V

V

A

V

R

R

A

G

F

V

V
x
M

K
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binding 5-[(1-carboxyvinyl)oxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: R39 (= R48), R45 (≠ A54), R48 (≠ T57), M49 (≠ A58), A133 (≠ G129), R134 (= R130)
binding flavin mononucleotide: H110 (= H106), A133 (≠ G129), N251 (≠ G238), A252 (= A239), M310 (≠ V292), K311 (= K293), I313 (= I295), P314 (= P296), T315 (≠ S297)

P0A2Y6 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
35% identity, 94% coverage: 14:345/354 of query aligns to 5:363/388 of P0A2Y6

query
sites
P0A2Y6

T

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NA 251:252 (≠ GA 238:239) binding FMN
G296 (= G278) binding FMN
KPIPT 311:315 (≠ KPIPS 293:297) binding FMN

Query Sequence

>WP_018125315.1 NCBI__GCF_000375485.1:WP_018125315.1
MSGNTFGELFTLTTFGESHGPGLGGVVDGCPAGIPMSEEIVQQELDRRKPGQGAASTARK
ESDTVRIYSGVFEGQTTGTPIGFVVENEDQRSRDYSRVKDVFRPGHADFTYHSKFGGVRD
YRGGGRSSGRETVARVAGGAVALEFLRSEGISVFAYTAEIGGISADPVDMENAQNNPFFS
PDGEVVEKWEERIREVKGLGDTLGGVVEVRIAGLAPGIGEPVFDKLDARLAYALMGVGAV
KAVEIGSGCDAARKLGSENNDYMTTAGFSSNNAGGILGGISSGQDVVARAFVKPIPSIAQ
EQHCMDVEGKRVCMRIGGRHDICAIPRINPVLKAMTALTLADLILRDRRLGVRP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory