SitesBLAST
Comparing WP_018125376.1 NCBI__GCF_000375485.1:WP_018125376.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4whxA X-ray crystal structure of an amino acid aminotransferase from burkholderia pseudomallei bound to the co-factor pyridoxal phosphate
55% identity, 99% coverage: 1:304/308 of query aligns to 2:305/306 of 4whxA
1iyeA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
54% identity, 97% coverage: 4:302/308 of query aligns to 1:300/304 of 1iyeA
- active site: F33 (= F36), G35 (= G38), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: R56 (= R60), Y92 (= Y96), Y126 (= Y129), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), L214 (= L216), G216 (= G218), I217 (≠ L219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)
1iydA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase (see paper)
54% identity, 97% coverage: 4:302/308 of query aligns to 1:300/304 of 1iydA
- active site: F33 (= F36), G35 (= G38), K156 (= K159), A157 (= A160), E190 (= E193), L214 (= L216)
- binding glutaric acid: Y92 (= Y96), Y126 (= Y129), A255 (= A257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L216), G216 (= G218), I217 (≠ L219), T218 (= T220), T254 (= T256)
1i1mA Crystal structure of escherichia coli branched-chain amino acid aminotransferase. (see paper)
54% identity, 97% coverage: 4:302/308 of query aligns to 1:300/304 of 1i1mA
- active site: K156 (= K159)
- binding 4-methyl valeric acid: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (= A257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), L214 (= L216), G216 (= G218), I217 (≠ L219), T218 (= T220), G253 (= G255), T254 (= T256)
1i1lA Crystal structure of eschelichia coli branched-chain amino acid aminotransferase. (see paper)
54% identity, 97% coverage: 4:302/308 of query aligns to 1:300/304 of 1i1lA
- active site: K156 (= K159)
- binding 2-methylleucine: Y92 (= Y96), K156 (= K159), T254 (= T256), A255 (= A257)
- binding pyridoxal-5'-phosphate: R56 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), G216 (= G218), I217 (≠ L219), T218 (= T220), T254 (= T256)
2ej0B Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase with pyridoxamine 5'-phosphate
54% identity, 96% coverage: 8:302/308 of query aligns to 7:301/305 of 2ej0B
- active site: F35 (= F36), G37 (= G38), K158 (= K159), E192 (= E193), L215 (= L216)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R58 (= R60), Y163 (= Y164), E192 (= E193), G195 (= G196), E196 (= E197), L215 (= L216), G217 (= G218), I218 (≠ L219), T219 (= T220), G254 (= G255), T255 (= T256)
2ej3A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with gabapentin
52% identity, 96% coverage: 8:302/308 of query aligns to 7:293/297 of 2ej3A
- active site: F35 (= F36), G37 (= G38), K150 (= K159), E184 (= E193), L207 (= L216)
- binding [1-(aminomethyl)cyclohexyl]acetic acid: G187 (= G196), G246 (= G255), T247 (= T256), A248 (= A257)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (≠ L219), T211 (= T220), G246 (= G255), T247 (= T256)
2eiyA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with 4-methylvaleric acid
52% identity, 96% coverage: 8:302/308 of query aligns to 7:293/297 of 2eiyA
- active site: F35 (= F36), G37 (= G38), K150 (= K159), E184 (= E193), L207 (= L216)
- binding 4-methyl valeric acid: F35 (= F36), Y94 (= Y96), T247 (= T256), A248 (= A257)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), E188 (= E197), L207 (= L216), G209 (= G218), I210 (≠ L219), T211 (= T220), G246 (= G255), T247 (= T256)
1wrvA Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase
52% identity, 96% coverage: 8:302/308 of query aligns to 7:293/297 of 1wrvA
- active site: F35 (= F36), G37 (= G38), K150 (= K159), E184 (= E193), L207 (= L216)
- binding pyridoxal-5'-phosphate: R58 (= R60), K150 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), L207 (= L216), G209 (= G218), I210 (≠ L219), T211 (= T220), T247 (= T256)
2ej2A Crystal structure of t.Th.Hb8 branched-chain amino acid aminotransferase complexed with n-(5'-phosphopyridoxyl)-l-glutamate
52% identity, 96% coverage: 8:302/308 of query aligns to 7:290/294 of 2ej2A
- active site: F35 (= F36), G37 (= G38), K147 (= K159), E181 (= E193), L204 (= L216)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: R58 (= R60), Y94 (= Y96), Y152 (= Y164), E181 (= E193), G184 (= G196), E185 (= E197), L204 (= L216), G206 (= G218), I207 (≠ L219), T208 (= T220), T244 (= T256), A245 (= A257)
6thqB Crystal structure of branched-chain aminotransferase from thermophilic archaea thermoproteus uzoniensis with norvaline
47% identity, 97% coverage: 5:302/308 of query aligns to 6:297/301 of 6thqB
- active site: F37 (= F36), K156 (= K159), E190 (= E193), L214 (= L216)
- binding pyridoxal-5'-phosphate: R60 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), N195 (= N198), L214 (= L216), G216 (= G218), I217 (≠ L219), T218 (= T220), T254 (= T256)
- binding 2-[o-phosphonopyridoxyl]-amino-pentanoic acid: R60 (= R60), Y97 (= Y96), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), E194 (= E197), N195 (= N198), G216 (= G218), I217 (≠ L219), T218 (= T220), G253 (= G255), T254 (= T256), A255 (= A257)
5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
46% identity, 94% coverage: 7:294/308 of query aligns to 3:284/290 of 5mr0D
- active site: F32 (= F36), G34 (= G38), K150 (= K159), E183 (= E193), L206 (= L216)
- binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R60), G100 (= G109), L101 (≠ V110), K150 (= K159), Y154 (= Y164), E183 (= E193), G186 (= G196), D187 (≠ E197), L206 (= L216), I209 (≠ L219), T210 (= T220), G245 (= G255), T246 (= T256)
5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
45% identity, 95% coverage: 8:301/308 of query aligns to 5:290/290 of 5e25A
- active site: F33 (= F36), G35 (= G38), K151 (= K159), E184 (= E193), L207 (= L216)
- binding 2-oxoglutaric acid: Y88 (= Y96), K151 (= K159), T247 (= T256), A248 (= A257)
- binding pyridoxal-5'-phosphate: R52 (= R60), K151 (= K159), Y155 (= Y164), E184 (= E193), G187 (= G196), D188 (≠ E197), L207 (= L216), G209 (= G218), I210 (≠ L219), T211 (= T220), G246 (= G255), T247 (= T256)
6q8eA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum in pmp-form (see paper)
36% identity, 97% coverage: 3:302/308 of query aligns to 1:301/307 of 6q8eA
- active site: F34 (= F36), K156 (= K159), E190 (= E193), L214 (= L216)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ E197), C195 (≠ N198), L214 (= L216), S216 (≠ G218), I217 (≠ L219), T218 (= T220), G254 (= G255), T255 (= T256)
7neaA Crystal structure of branched-chain amino acid aminotransferase from thermobaculum terrenum (m3 mutant). (see paper)
35% identity, 97% coverage: 3:302/308 of query aligns to 1:301/309 of 7neaA
- active site: F34 (= F36), K156 (= K159), E190 (= E193), L214 (= L216)
- binding pyridoxal-5'-phosphate: R59 (= R60), K156 (= K159), Y161 (= Y164), E190 (= E193), G193 (= G196), S194 (≠ E197), L214 (= L216), S216 (≠ G218), I217 (≠ L219), T218 (= T220), T255 (= T256)
6h65C Crystal structure of the branched-chain-amino-acid aminotransferase from haliangium ochraceum
35% identity, 96% coverage: 7:302/308 of query aligns to 6:303/308 of 6h65C
- active site: F35 (= F36), K158 (= K159), E192 (= E193), L216 (= L216)
- binding pyridoxal-5'-phosphate: R60 (= R60), K158 (= K159), Y163 (= Y164), E192 (= E193), A196 (≠ E197), L216 (= L216), S218 (≠ G218), V219 (≠ L219), T220 (= T220), G256 (= G255), T257 (= T256)
7p3tB Transaminase of gamma-proteobacterium (see paper)
30% identity, 92% coverage: 8:290/308 of query aligns to 6:282/299 of 7p3tB
- binding pyridoxal-5'-phosphate: R53 (= R60), K153 (= K159), R157 (≠ Y164), E186 (= E193), S187 (≠ G194), A188 (≠ S195), A189 (≠ G196), S190 (≠ E197), G210 (= G218), I211 (≠ L219), T212 (= T220), T248 (= T256)
3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
33% identity, 96% coverage: 9:304/308 of query aligns to 17:333/335 of 3ht5A
- active site: K171 (= K159)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R60), K171 (= K159), Y176 (= Y164), E207 (= E193), G210 (= G196), M211 (≠ E197), N212 (= N198), L235 (= L216), G237 (= G218), I238 (≠ L219), T239 (= T220), T281 (= T256)
3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
30% identity, 91% coverage: 7:287/308 of query aligns to 2:272/280 of 3lqsA
- active site: Y31 (≠ F36), V33 (≠ G38), K145 (= K159), E177 (= E193), L201 (= L216)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (≠ G38), R50 (= R60), E177 (= E193), S180 (≠ G196), S181 (≠ E197), N182 (= N198), L201 (= L216), G203 (= G218), I204 (≠ L219), T205 (= T220), S240 (≠ G255), T241 (= T256), T242 (≠ A257)
P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
30% identity, 91% coverage: 7:287/308 of query aligns to 3:273/283 of P19938
- Y32 (≠ F36) binding substrate
- R51 (= R60) binding pyridoxal 5'-phosphate
- R99 (≠ M108) binding substrate
- H101 (≠ V110) binding substrate
- K146 (= K159) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
- E178 (= E193) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
- L202 (= L216) mutation to A: Inactivates enzyme.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Query Sequence
>WP_018125376.1 NCBI__GCF_000375485.1:WP_018125376.1
MVQQSEYIWFDGELVPWEQANVHVLTHALHYGTGVFEGIRAYECTDGSSEVFRLKEHMVR
LLDSAKILGIKVPYSLEELVQATDETLKANKLKGAYVRPLVFVGDGAMGVHPGNNPIRVC
IATWPWGAYLGDEALEKGIRVRCSSYTRHHVNVMMTKAKACGNYVNSVLAKTEAVADGYD
EAILLDTTGHVAEGSGENIFMVKDEVLYTPHLSQVLGGLTRDSVIQLASDLGYEVREMAI
GRDMLYTADEVFFTGTAAELTPIREIDRRQVGEGKAGDVTKALQTEFFKILKGENEDYEH
WLHRYSVD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory