SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_018231161.1 NCBI__GCF_000378965.1:WP_018231161.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P16703 Cysteine synthase B; CSase B; O-acetylserine (thiol)-lyase B; OAS-TL B; O-acetylserine sulfhydrylase B; EC 2.5.1.47 from Escherichia coli (strain K12) (see paper)
67% identity, 98% coverage: 5:294/296 of query aligns to 3:292/303 of P16703

query
sites
P16703

T

T

L

L

E

E

S

Q

C

T

V

I

G

G

N

N

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K

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T

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L

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M

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Y

Y

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E

D

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Y

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E

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K

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L

D

D

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Q

F

F

S

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D

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N

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Y

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A

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Y

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M

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W

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Y

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A

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F

N71 (= N73) binding pyridoxal 5'-phosphate
S255 (= S257) binding pyridoxal 5'-phosphate

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
67% identity, 98% coverage: 5:294/296 of query aligns to 3:292/294 of 2bhtA

query
sites
2bhtA

T

T

L

L

E

E

S

Q

C

T

V

I

G

G

N

N

T

T

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P

L

L

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K

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L

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G

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T

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N

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T

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K

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E

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G

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K

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F

active site: K41 (= K43), S69 (= S71), Q199 (≠ T201), G203 (= G205), S255 (= S257), C280 (= C282)
binding pyridoxal-5'-phosphate: K41 (= K43), N71 (= N73), M173 (= M175), G174 (= G176), T175 (= T177), T176 (= T178), T178 (= T180), G208 (= G210), S255 (= S257), C280 (= C282)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
45% identity, 96% coverage: 10:294/296 of query aligns to 12:306/310 of 5xoqA

query
sites
5xoqA

V

I

G

G

N

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T

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L

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L

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K

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Y

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C

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A

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G

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T

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Q

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K

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N

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K

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K

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G

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x
R

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x
H

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K

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x
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R
x
G

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I

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E
x
A

A

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D

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L

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E

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C

C

G

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C

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G

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A

A

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M

A

A

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G

A

A

L

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K

L

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S

A

R

A

-

R

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P

E

E

L

F

E

A

N

G

A

K

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I

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I

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C

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D

D

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G

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T

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F

binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), M123 (= M121), Q144 (= Q142), R218 (vs. gap), H219 (vs. gap), Q222 (≠ R212), G223 (≠ R213), A226 (≠ E216)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
44% identity, 96% coverage: 10:294/296 of query aligns to 11:306/309 of 7n2tA

query
sites
7n2tA

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

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D

E

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T

C

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V

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A

T

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V

A

K

K

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L

E

E

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M

N

M

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E

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P

A

C

G

S

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K

K

D

D

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Y

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M

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D

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E

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N

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K

G

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P

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G

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V

L

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S

G

G

N

N

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T

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G

I

I

A

G

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L

A

A

M

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A

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A

K

K

G

G

Y

Y

R

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M

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L

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C

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M

P

P

E

A

N

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M

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S

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L

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K

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A

A

F

F

G

G

A

A

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E

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L

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P

E

A

Q

R

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G

M

M

E

K

G

G

A

A

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D

Q

L

K

A

A

K

E

Q

E

M

I

E

K

A

A

E

K

G

T

-

P

R

N

G

S

R

Y

V

I

L

L

D

Q

Q

Q

F

F

S

E

N

N

P

P

D

A

N

N

P

P

L

K

S

I

H

H

Y

Y

E

E

G

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T

T

G

G

P

P

E

E

I

I

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D

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G

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G

G

K

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A

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M

I

G

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Y

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L

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K

E

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Q

D

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K

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A

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I

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K

R

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W

G

P

P

E

H

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K

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I

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Q

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G

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I

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G

-

A

A

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Y

F

L

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G

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K

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E

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A

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A

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R
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R

L

I

S

A

R

K

E
x
R

L

P

E

E

N

N

A

A

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G

-

K

V

L

I

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A

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F

binding phosphate ion: R277 (= R267), R281 (≠ E271)

Q93244 Cysteine synthase 1; O-acetylserine (thiol)-lyase 1; OAS-TL; EC 2.5.1.47 from Caenorhabditis elegans (see 2 papers)
43% identity, 97% coverage: 8:294/296 of query aligns to 13:310/341 of Q93244

query
sites
Q93244

S

A

C

L

V

I

G

G

N

N

T

T

P

P

L

M

V

V

R

Y

L

I

Q

N

R

K

L

L

P

T

G

K

E

G

T

L

S

P

N

G

T

T

I

V

L

A

V

V

K

K

L

I

E

E

G

Y

N

M

N

N

P

P

A

A

G

G

S

S

V

V

K

K

D

D

R

R

P

I

A

G

L

A

G

A

M

M

I

L

R

A

K

A

A

A

E

E

E

K

R

D

G

G

D

T

I

V

R

I

P

P

G

G

-

V

D

T

T

T

L

L

V

I

E

E

A
x
P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

A

L

L

A

A

M

F

V

V

A
|
A

A

A

M

A

K

K

G

G

Y

Y

R

R

M

C

V

I

L

V

I

T

M

M

P

P

E

A

N

S

M

M

S

S

A

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E

E

R

R

A

T

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L

M

L

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K

A

A

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Y

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G

A

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E

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L

L

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P

E

A

Q

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G

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M

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G

A

A

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I

D

D

L

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A

A

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N

Q

Q

M

L

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K

A

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I

R

P

G

G

R
x
S

-

I

V

I

L

L

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A

Q

Q

F

F

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D

N

N

P

P

D

N

N

N

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P

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L

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H

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Y

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x
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G

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I

M

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G

T

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G

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R

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Y

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E

E

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G

A

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E

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A

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I

L

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S

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G

I

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R

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R

A

W

G

P

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-

K

-

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Q

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x
G

-

A

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A

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P

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E

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A

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M

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x
R

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L

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A

Y

R

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G

I

L

F

L

C

G

G

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V

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S

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|
S

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A

A

N

V

V

A

A

L

L

R

Q

L

L

S

A

R

A

-

R

-

P

E

E

L

M

E

A

N

G

A

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L

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x
T

I

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D

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R

C

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G

D

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Y

Y

I

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T

T

S

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P

V

L

F

Y

P75 (≠ A69) mutation to L: In n5537; severe loss of protein stability.
A88 (= A82) mutation to V: In n5522; severe loss of protein stability.
S144 (≠ R138) mutation to F: In mr26; susceptible to high levels of hydrogen sulfide.
G181 (≠ A174) mutation to E: In n5521 and mr23; severe loss of protein stability. Susceptible to high levels of hydrogen sulfide.
G183 (= G176) mutation to R: In n5515; severe loss of protein stability.
G229 (vs. gap) mutation to E: In mr33; susceptible to high levels of hydrogen sulfide.
R259 (≠ L245) mutation to K: In n5519; no loss of protein stability. No effect on enzyme activity.
S272 (= S258) mutation to F: In mr29; susceptible to high levels of hydrogen sulfide.
T295 (≠ S279) mutation to I: In mr39; susceptible to high levels of hydrogen sulfide.

3vbeC Crystal structure of beta-cyanoalanine synthase in soybean (see paper)
42% identity, 98% coverage: 6:294/296 of query aligns to 15:314/329 of 3vbeC

query
sites
3vbeC

L

V

E

S

S

Q

C

L

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

K

L

V

P

T

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E

E

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C

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G

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A

T

Y

I

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V

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K

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N

M

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K

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D

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R

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A

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M

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G

N

D

L

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I

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P

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G

D

K

T

T

-

T

L

L

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E

A

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S

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N

T

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G

I

I

A

S

L

M

A

A

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F

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M

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K

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G

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Y

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V

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M

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Y

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A

L

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A

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M

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R

A

A

F

F

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G

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I

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Q

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G

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M

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G

G

G

A

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V

D

K

L

K

A

A

K

Y

Q

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L

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L

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E

A

N

E

T

G

P

R

N

G

A

R

H

V

M

L

L

D

Q

Q

Q

F

F

S

S

N

N

P

P

D

A

N

N

P

T

L

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V

H

H

Y

F

E

E

G

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T

T

G

G

P

P

E

E

I

I

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W

R

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D

D

T

T

R

N

G

G

G

Q

I

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D

H

I

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F

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V

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x
M

A

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M

I

G
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G

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x
S

T
x
G

G

G

T
|
T

I

V

M

S

G

G

T

V

S

G

R

Q

Y

Y

L

L

K

K

E

S

Q

K

D

N

P

P

S

N

V

V

E

K

V

I

V

Y

G

G

V

V

T
x
E

P

P

A

S

E

E

G
x
S

A

N

S

V

I

L

P

N

G

G

I

G

R

K

R

P

W

G

P

P

E

H

-

H

-

I

-

T

-
x
G

-

N

-

G

-

V

A

G

Y

F

L

K

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P

G

D

I

I

F

L

E

D

D

L

F

D

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T

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L

L

E

E

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Q

S

P

E

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A

A

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D

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T

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M

A

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R

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L

L

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A

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K

E

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G

I

L

F

M

C

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G

G

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|
S

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S

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G

G

A

A

N

V

T

A

V

A

A

L

L

R

R

L

L

S

A

R

Q

E

L

L

P

E

E

N

N

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K

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A

K

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L

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I

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x
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L

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F

active site: K52 (= K43), S81 (= S71), E212 (≠ T201), S216 (≠ G205), S275 (= S257), P302 (≠ C282)
binding pyridoxal-5'-phosphate: K52 (= K43), N83 (= N73), M184 (≠ S173), G187 (= G176), S188 (≠ T177), G189 (≠ T178), T191 (= T180), G231 (vs. gap), S275 (= S257), P302 (≠ C282)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
44% identity, 97% coverage: 10:296/296 of query aligns to 11:308/320 of 2isqA

query
sites
2isqA

V

I

G

G

N

N

T

T

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P

L

L

V

V

R

Y

L

L

Q

N

R

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L

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E

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G

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K

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L

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M

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K

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D

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M

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-

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L

L

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T

S
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S

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G

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N

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T

G

G

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G

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L

A

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L

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x
K

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A

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T

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N

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Q

Q

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F

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N

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H

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Y

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P

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I

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W

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G

G

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H

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F

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x
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M

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G

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T

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x
G

G

G

T
|
T

I

I

M

T

G

G

T

A

S

G

R

K

Y

Y

L

L

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K

E

E

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Q

D

N

P

A

S

N

V

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E

K

V

L

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A

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G

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G

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x
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P

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x
H

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K

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x
Q

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x
G

-

I

-

G

-

A

A

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D

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D

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V

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D

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E

A

S

E

I

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M

A

R

R

L

Q

L

L

A

A

A

L

R

K

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

L

S

A

R

Q

E

R

L

P

E

E

N

N

A

A

-

G

-

K

V

L

I

F

V

V

S

A

I

I

I

F

C
x
P

D

S

R

F

G

G

D

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R

R

Y

Y

I

L

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S

T

T

G

V

V

L

F

F

P

D

A

A

active site: K44 (= K43), S267 (= S257)
binding pyridoxal-5'-phosphate: K44 (= K43), N75 (= N73), G177 (≠ A174), G179 (= G176), T180 (= T177), G181 (≠ T178), T183 (= T180), G223 (vs. gap), S267 (= S257), P294 (≠ C282)
binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), G122 (= G120), M123 (= M121), K124 (≠ E122), G217 (≠ W214), P218 (= P215), H219 (≠ E216), Q222 (vs. gap), G223 (vs. gap)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
44% identity, 97% coverage: 10:296/296 of query aligns to 13:310/322 of P47998

query
sites
P47998

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

N

L

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A

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E

E

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N

G

T

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A

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A

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K

L

L

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E

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M

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E

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K

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D

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A

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G

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-

V

L

L

V

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A

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T
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T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

V

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A

A

M

A

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K

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Y

Y

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M

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M

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S

A

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L

A

A

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A

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A

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A

A

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A

A

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T

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P

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N

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G

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V

M

L

L

D

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Q
|
Q

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F

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E

N

N

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A

N

N

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P

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H
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H

Y

Y

E

E

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G
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G

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P

E

E

I

I

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W

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D

G

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G

G

G

G

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F

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G

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T

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x
G

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G

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T

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T

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G

T

A

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G

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K

Y

Y

L

L

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K

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Q

D

N

P

A

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N

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E

K

V

L

V

Y

G

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P

P

A

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E

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A

A

S

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I

L

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S

G

G

I

G

R
x
K

R

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W

G

P

P

E
x
H

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x
K

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

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I

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G

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F

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F

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R

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D

D

R

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L

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S

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D

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E

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S

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I

D

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M

M

A

R

R

L

Q

L

L

A

A

A

L

R

K

E

E

G

G

I

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F

L

C

V

G

G

V

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|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

L

S

A

R

Q

E

R

L

P

E

E

N

N

A

A

-

G

-

K

V

L

I

F

V

V

S

A

I

I

I

F

C

P

D

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R

F

G

G

D

E

R

R

Y

Y

I

L

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S

T

T

G

V

V

L

F

F

P

D

A

A

K46 (= K43) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T70) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S71) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N73) binding pyridoxal 5'-phosphate; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T74) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q142) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H152) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G157) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (≠ GTTGT 176:180) binding pyridoxal 5'-phosphate
T182 (= T177) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T180) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (≠ R212) mutation to A: Impaired interaction with SAT1.
H221 (≠ E216) mutation to A: Impaired interaction with SAT1.
K222 (vs. gap) mutation to A: Impaired interaction with SAT1.
S269 (= S257) binding pyridoxal 5'-phosphate; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

3vc3A Crystal structure of beta-cyanoalanine synthase k95a mutant in soybean (see paper)
42% identity, 98% coverage: 6:294/296 of query aligns to 8:307/322 of 3vc3A

query
sites
3vc3A

L

V

E

S

S

Q

C

L

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

K

L

V

P

T

G

E

E

G

T

C

S

G

N

A

T

Y

I

V

L

A

V

V

K

K

L

Q

E

E

G

M

N

M

N

Q

P

P

A

T

G

A

S

S

V

I

K
x
A

D

D

R

R

P

P

A

A

L

Y

G

A

M

M

I

I

R

T

K

D

A

A

E

E

R

K

G

N

D

L

I

I

R

T

P

P

G

G

D

K

T

T

-

T

L

L

V

I

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
x
M

G

G

I

I

A

S

L

M

A

A

M

F

V

M

A

A

M

M

K

K

G

G

Y

Y

R

K

M

M

V

V

L

L

I

T

M

M

P

P

E

S

N

Y

M

T

S

S

A

L

E

E

R

R

A

V

S

T

M

M

K

R

A

A

F

F

G

G

A

A

E

E

I

L

I

I

L

L

V

T

S

D

R

P

E

A

Q

K

G

G

M

M

E

G

G

G

A

T

R

V

D

K

L

K

A

A

K

Y

Q

E

M

L

-

L

E

E

A

N

E

T

G

P

R

N

G

A

R

H

V

M

L

L

D

Q

Q
|
Q

F

F

S

S

N

N

P

P

D

A

N

N

P

T

L

Q

S

V

H

H

Y

F

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

D

T

T

R

N

G

G

G

Q

I

V

T

D

H

I

F

F

V

V

S
x
M

A

G

M

I

G
|
G

T
x
S

T
x
G

G

G

T
|
T

I

V

M

S

G

G

T

V

S

G

R

Q

Y

Y

L

L

K

K

E

S

Q

K

D

N

P

P

S

N

V

V

E

K

V

I

V

Y

G

G

V

V

T

E

P

P

A

S

E

E

G

S

A

N

S

V

I

L

P

N

G

G

I

G

R

K

R

P

W

G

P

P

E

H

-

H

-

I

-

T

-
x
G

-

N

-

G

-

V

A

G

Y

F

L

K

P

P

G

D

I

I

F

L

E

D

D

L

F

D

R

V

V

M

D

E

R

K

T

V

L

L

E

E

V

V

T

S

Q

S

P

E

E

D

A

A

E

V

D

N

T

M

M

A

R

R

L

V

L

L

A

A

A

L

R

K

E

E

G

G

I

L

F

M

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

N

V

T

A

V

A

A

L

L

R

R

L

L

S

A

R

Q

E

L

L

P

E

E

N

N

-

K

-

G

A

K

V

L

I

I

V

V

S

T

I

V

I

H

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

I

L

S

S

T

S

G

V

V

L

F

F

active site: A45 (≠ K43), S268 (= S257), P295 (≠ C282)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: T73 (= T70), S74 (= S71), N76 (= N73), M77 (≠ T74), Q146 (= Q142), M177 (≠ S173), G180 (= G176), S181 (≠ T177), G182 (≠ T178), T184 (= T180), G224 (vs. gap), S268 (= S257), P295 (≠ C282)

8b9yC Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
42% identity, 98% coverage: 4:294/296 of query aligns to 12:312/330 of 8b9yC

query
sites
8b9yC

P

P

T

S

L

M

E

D

S

A

C

L

V

I

G

G

N

E

T

T

P

P

L

A

V

V

R

Y

L

L

Q

K

R

R

L

M

P

-

G

N

E

D

T

T

S

A

N

A

T

T

I

I

L

V

V

L

K

K

L

L

E

E

G

C

N

E

N

N

P

P

A

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

G

A

M

I

I

Y

R

D

K

K

A

A

E

E

E

K

R

E

G

G

D

K

I

I

R

I

P

P

G

G

D

K

T

S

-

V

L

I

V

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

H

V

I

A

G

A

T

M

I

K

R

G

G

Y

Y

R

K

M

V

V

I

L

I

I

V

M

M

P

P

E

E

N

S

M

M

S

S

A

I

E

E

R

R

A

C

S

L

M

M

K

R

A

I

F

F

G

G

A

A

E

E

I

V

I

I

L

L

V

T

S

P

R

A

E

A

Q

L

G

G

M

M

E

K

G

G

A

A

R

L

D

E

L

A

A

V

K

N

Q

R

M

I

E

V

A

S

E

N

G

N

R

P

G

D

R

A

V

V

-

S

L

A

D

N

Q

Q

F

F

S

A

N

T

P

K

D

Y

N

N

P

A

L

Q

S

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

R

K

G

G

G

H

I

V

T

D

H

C

F

F

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

R

Y

Y

L

L

K

K

E

S

Q

V

D

G

P

C

S

G

V

A

E

T

V

I

V

F

G

A

V

V

T

E

P

P

A

A

E

E

G

S

A

P

S

V

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

E

H

-

R

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

L

V

P

P

G

E

I

V

F

F

E

E

D

A

F

A

R

L

V

V

D

D

R

E

T

V

L

I

E

Q

V

V

T

S

Q

G

P

D

E

E

A

A

E

I

D

D

T

T

M

A

R

Q

L

K

L

L

A

P

A

R

R

T

E

D

G

G

I

I

F

F

C

C

G

G

V

F

S
|
S

S

G

G

G

G

A

A

N

V

V

A

Y

A

A

L

L

R

Q

L

I

S

A

R

K

-

R

-

P

E

E

L

M

E

A

N

G

A

K

V

T

I

I

V

V

S

T

I

V

I

I

C
x
P

D
x
S

R

Y

G

G

D

E

R

R

Y

Y

I

L

S

S

T

T

G

A

V

L

F

Y

binding pyridoxal-5'-phosphate: K50 (= K43), N81 (= N73), G185 (= G176), T186 (= T177), G187 (≠ T178), T189 (= T180), G229 (vs. gap), S273 (= S257), P300 (≠ C282), S301 (≠ D283)

8b9yA Cysteine synthase from trypanosoma cruzi with plp and oas (see paper)
42% identity, 98% coverage: 4:294/296 of query aligns to 10:295/313 of 8b9yA

query
sites
8b9yA

P

P

T

S

L

M

E

D

S

A

C

L

V

I

G

G

N

E

T

T

P

P

L

A

V

V

R

Y

L

L

Q

K

R

R

L

M

P

-

G

N

E

D

T

T

S

A

N

A

T

T

I

I

L

V

V

L

K

K

L

L

E

E

G
x
C

N
x
E

N
|
N

P
|
P

A

M

G
x
A

S

S

V
|
V

K
|
K

D

D

R

R

P

L

A

A

L

Y

G

A

M

I

I

Y

R

D

K

K

A

A

E

E

E

K

R

E

G

G

D

K

I

I

R

I

P

P

G

G

D

K

T

S

-

V

L

I

V

V

E

E

A

A

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

H

V

I

A

G

A

T

M

I

K

R

G

G

Y

Y

R

K

M

V

V

I

L

I

I

V

M

M

P

P

E

E

N

S

M

M

S

S

A

I

E

E

R

R

A

C

S

L

M

M

K

R

A

I

F

F

G

G

A

A

E

E

I

V

I

I

L

L

V

T

S

P

R

A

E

A

Q

L

G

G

M

M

E

K

G

G

A

A

R

L

D

E

L

A

A

V

K

N

Q

R

M

I

E

V

A

S

E

N

G

N

R

P

G

D

R

A

V

V

-

S

L

A

D

N

Q

Q

F

F

S

A

N

T

P

K

D

Y

N

N

P

A

L

Q

S

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

R

K

G

G

G

H

I

V

T

D

H

C

F

F

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

R

Y

Y

L

L

K

K

E

S

Q

V

D

G

P

C

S

G

V

A

E

T

V

I

V

F

G

A

V

V

T

E

P

P

A

A

E

E

G

S

A

P

S

V

I

L

P

-

G

-

I

-

R

-

W

-

P

-

E

-

A

S

Y

F

L

V

P

P

G

E

I

V

F

F

E

E

D

A

F

A

R

L

V

V

D

D

R

E

T

V

L

I

E

Q

V

V

T

S

Q

G

P

D

E

E

A

A

E

I

D

D

T

T

M

A

R

Q

L

K

L

L

A

P

A

R

R

T

E

D

G

G

I

I

F

F

C

C

G

G

V

F

S
|
S

S

G

G

G

G

A

A

N

V

V

A

Y

A

A

L

L

R

Q

L

I

S

A

R

K

-

R

-

P

E

E

L

M

E

A

N

G

A

K

V

T

I

I

V

V

S

T

I

V

I

I

C
x
P

D
x
S

R
x
Y

G

G

D

E

R

R

Y

Y

I

L

S

S

T

T

G

A

V

L

F

Y

binding o-acetylserine: T76 (= T70), G78 (= G72), N79 (= N73), T80 (= T74)
binding pyridoxal-5'-phosphate: V47 (= V42), K48 (= K43), N79 (= N73), G183 (= G176), T184 (= T177), G185 (≠ T178), T187 (= T180), S256 (= S257), P283 (≠ C282), S284 (≠ D283)
binding alpha-D-ribofuranose: C40 (≠ G35), E41 (≠ N36), N42 (= N37), P43 (= P38), A45 (≠ G40), Y285 (≠ R284)

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
43% identity, 97% coverage: 10:296/296 of query aligns to 11:308/320 of 1z7yA

query
sites
1z7yA

V

I

G

G

N

N

T

T

P

P

L

L

V

V

R

Y

L

L

Q

N

R

N

L

V

P

A

G

E

E

G

T

C

S

V

N

G

T

R

I

V

L

A

V

A

K

K

L

L

E

E

G

M

N

M

N

E

P

P

A

C

G

S

S

S

V

V

K
x
A

D

D

R

R

P

I

A

G

L

F

G

S

M

M

I

I

R

S

K

D

A

A

E

E

E

K

R

K

G

G

D

L

I

I

R

K

P

P

G

G

D

E

T

S

-

V

L

L

V

I

E

E

A

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

A

G

L

L

A

A

M

F

V

T

A

A

M

A

K

K

G

G

Y

Y

R

K

M

L

V

I

L

I

I

T

M

M

P

P

E

A

N

S

M

M

S

S

A

T

E

E

R

R

A

I

S

I

M

L

K

L

A

A

F

F

G

G

A

V

E

E

I

L

I

V

L

L

V

T

S

D

R

P

E

A

Q

K

G

G

M

M

E

K

G

G

A

A

R

I

D

A

L

K

A

A

K

E

Q

E

M

I

E

L

A

A

E

K

G

T

-

P

R

N

G

G

R

Y

V

M

L

L

D

Q

Q
|
Q

F

F

S

E

N

N

P

P

D

A

N

N

P

P

L

K

S

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

K

D

G

T

T

R

G

G

G

G

K

I

I

T

D

H

G

F

F

V

V

S

S

A

G

M
x
I

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

A

S

G

R

K

Y

Y

L

L

K

K

E

E

Q

Q

D

N

P

A

S

N

V

V

E

K

V

L

V

Y

G

G

V

V

T

E

P

P

A

V

E

E

G

S

A

A

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

E

H

-

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

L

I

P

P

G

S

I

V

F

L

E

N

D

V

F

D

R

L

V

I

D

D

R

E

T

V

L

V

E

Q

V

V

T

S

Q

S

P

D

E

E

A

S

E

I

D

D

T

M

M

A

R

R

L

Q

L

L

A

A

A

L

R

K

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

L

S

A

R

Q

E

R

L

P

E

E

N

N

A

A

-

G

-

K

V

L

I

F

V

V

S

A

I

I

I

F

C
x
P

D
x
S

R

F

G

G

D

E

R

R

Y

Y

I

L

S

S

T

T

G

V

V

L

F

F

P

D

A

A

active site: A44 (≠ K43), S267 (= S257)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G72), N75 (= N73), T76 (= T74), Q145 (= Q142), I178 (≠ M175), G179 (= G176), T180 (= T177), G181 (≠ T178), T183 (= T180), G223 (vs. gap), S267 (= S257), P294 (≠ C282), S295 (≠ D283)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
44% identity, 98% coverage: 6:294/296 of query aligns to 3:301/302 of 2efyA

query
sites
2efyA

L

V

E

E

S

G

C

A

V

I

G

G

N

K

T

T

P

P

L

V

V

V

R

R

L

L

Q

A

R

K

L

V

P

V

G

E

E

P

T

D

S

M

N

A

T

E

I

V

L

W

V

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

G

Y

M

M

I

I

R

K

K

D

A

A

E

E

R

R

G

G

D

I

I

L

R

R

P

P

G

G

-

S

-

G

D

Q

T

V

L

I

V

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

V

I

A

A

M

S

K

R

G

G

Y

Y

R

R

M

L

V

I

L

L

I

T

M

M

P

P

E

A

N

Q

M

M

S

S

A

E

E

E

R

R

R

K

A

R

S

V

M

L

K

K

A

A

F

F

G

G

A

A

E

E

I

L

I

V

L

L

V

T

S

D

R

P

E

E

Q

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

K

L

Q

R

M

L

E

K

A

E

E

E

G

L

R

G

G

A

R

F

V

M

L

P

D

D

Q
|
Q

F

F

S

K

N

N

P

P

D

A

N

N

P

V

L

R

S

A

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

R

E

G

G

G

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A

G

M

S

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

R

D

I

P

P

S

H

V

V

E

K

V

V

V

I

G

A

V

V

T
x
E

P

P

A

A

E

R

G
x
S

A

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W
x
M

P

G

E
x
P

A

G

Y

F

L

I

P

P

G

E

I

N

F

L

E

D

D

L

F

S

R

L

V

L

D

D

R

G

T

V

L

I

E

Q

V

V

T

W

Q

E

P

E

E

D

A

A

E

F

D

P

T

L

M

A

R

R

L

R

L

L

A

A

A

R

R

E

E

E

G

G

I

L

F

F

C

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

A

W

A

A

L

L

R

Q

L

V

S

A

R

R

E

E

L

L

E

G

N

P

A

G

V

K

I

R

V

V

S

A

I

C

I

I

C

S

-
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

I

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (≠ T201), S204 (≠ G205), S263 (= S257)
binding 5-oxohexanoic acid: T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q142), G175 (= G176), G219 (≠ R213), M220 (≠ W214), P222 (≠ E216)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S173), G175 (= G176), T176 (= T177), G177 (≠ T178), T179 (= T180), G219 (≠ R213), S263 (= S257), P289 (vs. gap), D290 (= D283)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
44% identity, 98% coverage: 6:294/296 of query aligns to 3:301/302 of 2ecqA

query
sites
2ecqA

L

V

E

E

S

G

C

A

V

I

G

G

N

K

T

T

P

P

L

V

V

V

R

R

L

L

Q

A

R

K

L

V

P

V

G

E

E

P

T

D

S

M

N

A

T

E

I

V

L

W

V

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

G

Y

M

M

I

I

R

K

K

D

A

A

E

E

R

R

G

G

D

I

I

L

R

R

P

P

G

G

-

S

-

G

D

Q

T

V

L

I

V

V

E

E

A

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

V

I

A

A

M

S

K

R

G

G

Y

Y

R

R

M

L

V

I

L

L

I

T

M

M

P

P

E

A

N

Q

M

M

S

S

A

E

E

E

R

R

R

K

A

R

S

V

M

L

K

K

A

A

F

F

G

G

A

A

E

E

I

L

I

V

L

L

V

T

S

D

R

P

E

E

Q

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

K

L

Q

R

M

L

E

K

A

E

E

E

G

L

R

G

G

A

R

F

V

M

L

P

D

D

Q
|
Q

F

F

S

K

N

N

P

P

D

A

N

N

P

V

L

R

S

A

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

R

E

G

G

G

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A
x
G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

R

D

I

P

P

S

H

V

V

E

K

V

V

V

I

G

A

V

V

T
x
E

P

P

A

A

E

R

G
x
S

A

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W

M

P

G

E

P

A

G

Y

F

L

I

P

P

G

E

I

N

F

L

E

D

D

L

F

S

R

L

V

L

D

D

R

G

T

V

L

I

E

Q

V

V

T

W

Q

E

P

E

E

D

A

A

E

F

D

P

T

L

M

A

R

R

L

R

L

L

A

A

A

R

R

E

E

E

G

G

I

L

F

F

C

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

A

W

A

A

L

L

R

Q

L

V

S

A

R

R

E

E

L

L

E

G

N

P

A

G

V

K

I

R

V

V

S

A

I

C

I

I

C

S

-
x
P

D

D

R

G

G

G

D

W

R

K

Y

Y

I

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (≠ T201), S204 (≠ G205), S263 (= S257)
binding (3s)-3-hydroxybutanoic acid: K40 (= K43), G71 (= G72), T73 (= T74), Q141 (= Q142), G219 (≠ R213)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S173), G173 (≠ A174), G175 (= G176), T176 (= T177), T179 (= T180), G219 (≠ R213), S263 (= S257), P289 (vs. gap)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
44% identity, 98% coverage: 6:294/296 of query aligns to 3:301/302 of 2ecoA

query
sites
2ecoA

L

V

E

E

S

G

C

A

V

I

G

G

N

K

T

T

P

P

L

V

V

V

R

R

L

L

Q

A

R

K

L

V

P

V

G

E

E

P

T

D

S

M

N

A

T

E

I

V

L

W

V

V

K

K

L

L

E

E

G

G

N

L

N

N

P

P

A

G

G

G

S

S

V

I

K
|
K

D

D

R

R

P

P

A

A

L

W

G

Y

M

M

I

I

R

K

K

D

A

A

E

E

R

R

G

G

D

I

I

L

R

R

P

P

G

G

-

S

-

G

D

Q

T

V

L

I

V

V

E

E

A

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

V

I

A

A

M

S

K

R

G

G

Y

Y

R

R

M

L

V

I

L

L

I

T

M

M

P

P

E

A

N

Q

M

M

S

S

A

E

E

E

R

R

R

K

A

R

S

V

M

L

K

K

A

A

F

F

G

G

A

A

E

E

I

L

I

V

L

L

V

T

S

D

R

P

E

E

Q

R

G

R

M

M

E

L

G

A

A

A

R

R

D

E

L

E

A

A

K

L

Q

R

M

L

E

K

A

E

E

E

G

L

R

G

G

A

R

F

V

M

L

P

D

D

Q
|
Q

F

F

S

K

N

N

P

P

D

A

N

N

P

V

L

R

S

A

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

R

E

G

G

G

R

I

I

T

D

H

A

F

F

V

V

S
x
Y

A

G

M

S

G
|
G

T
|
T

T

G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

Y

L

L

K

K

E

E

Q

R

D

I

P

P

S

H

V

V

E

K

V

V

V

I

G

A

V

V

T
x
E

P

P

A

A

E

R

G
x
S

A

N

S

V

I

L

P

S

-

G

-

K

-

M

-

G

-

Q

-

H

G

G

I

F

R

Q

R
x
G

W

M

P

G

E

P

A

G

Y

F

L

I

P

P

G

E

I

N

F

L

E

D

D

L

F

S

R

L

V

L

D

D

R

G

T

V

L

I

E

Q

V

V

T

W

Q

E

P

E

E

D

A

A

E

F

D

P

T

L

M

A

R

R

L

R

L

L

A

A

A

R

R

E

E

E

G

G

I

L

F

F

C

L

G

G

V

M

S
|
S

S

S

G

G

A

I

V

V

A

W

A

A

L

L

R

Q

L

V

S

A

R

R

E

E

L

L

E

G

N

P

A

G

V

K

I

R

V

V

S

A

I

C

I

I

C

S

-
x
P

D
|
D

R

G

G

G

D

W

R

K

Y

Y

I

L

S

S

T

T

G

P

V

L

F

Y

active site: K40 (= K43), S70 (= S71), E200 (≠ T201), S204 (≠ G205), S263 (= S257)
binding 4-methyl valeric acid: K40 (= K43), T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q142), G175 (= G176), T176 (= T177), G219 (≠ R213)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ S173), G175 (= G176), T176 (= T177), T179 (= T180), G219 (≠ R213), S263 (= S257), P289 (vs. gap), D290 (= D283)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 98% coverage: 6:294/296 of query aligns to 7:305/310 of P9WP55

query
sites
P9WP55

L

I

E

T

S

Q

C

L

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

Q

R

R

R

L

V

P

T

G

D

E

G

T

A

S

V

N

A

T

D

I

I

L

V

V

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

G

A

M

M

I

L

R

Q

K

A

A

A

E

E

E

Q

R

A

G

G

D

L

I

I

R

K

P

P

G

D

D

T

T

I

L

I

V

L

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

A

C

A

A

M

A

K

R

G

G

Y

Y

R

R

M

C

V

V

L

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

A

L

E

E

R

R

A

M

S

L

M

L

K

R

A

A

F

Y

G

G

A

A

E

E

I

L

I

I

L

L

V

T

S

P

R

G

E

A

Q

D

G

G

M

M

E

S

G

G

A

A

R

I

D

A

L

K

A

A

K

E

Q

E

M

L

-

A

E

K

A

T

E

D

G

Q

R

R

G

Y

R

F

V

V

L

P

D

Q

Q

Q

F

F

S

E

N

N

P

P

D

A

N

N

P

P

L

A

S

I

H

H

Y

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

R

D

G

G

G

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

D

K

P

P

S

S

V

A

E

R

V

F

V

V

G

A

V

V

T

E

P

P

A

A

E

A

G

S

A

P

S

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

E

H

-

P

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

L

V

P

P

G

P

I

V

F

L

E

D

D

Q

F

D

R

L

V

V

D

D

R

E

T

I

L

I

E

T

V

V

T

G

Q

N

P

E

E

D

A

A

E

L

D

N

T

V

M

A

R

R

L

R

L

L

A

A

A

R

R

E

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

A

V

A

A

L

L

R

Q

L

V

S

A

R

R

E

R

L

P

E

E

N

N

A

A

-

G

-

K

V

L

I

I

V

V

S

V

I

V

I

L

C

P

D

D

R

F

G

G

D

E

R

R

Y

Y

I

L

S

S

T

T

G

P

V

L

F

F

K44 (= K43) modified: N6-(pyridoxal phosphate)lysine
N74 (= N73) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTTGT 176:180) binding pyridoxal 5'-phosphate
S266 (= S257) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
43% identity, 98% coverage: 6:294/296 of query aligns to 7:305/306 of 2q3dA

query
sites
2q3dA

L

I

E

T

S

Q

C

L

V

I

G

G

N

R

T

T

P

P

L

L

V

V

R

R

L

L

Q

R

R

R

L

V

P

T

G

D

E

G

T

A

S

V

N

A

T

D

I

I

L

V

V

A

K

K

L

L

E

E

G

F

N

F

N

N

P

P

A

A

G

N

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

V

G

A

M

M

I

L

R

Q

K

A

A

A

E

E

E

Q

R

A

G

G

D

L

I

I

R

K

P

P

G

D

D

T

T

I

L

I

V

L

E

E

A

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

V

V

A

C

A

A

M

A

K

R

G

G

Y

Y

R

R

M

C

V

V

L

L

I

T

M

M

P

P

E

E

N

T

M

M

S

S

A

L

E

E

R

R

A

M

S

L

M

L

K

R

A

A

F

Y

G

G

A

A

E

E

I

L

I

I

L

L

V

T

S

P

R

G

E

A

Q

D

G

G

M

M

E

S

G

G

A

A

R

I

D

A

L

K

A

A

K

E

Q

E

M

L

-

A

E

K

A

T

E

D

G

Q

R

R

G

Y

R

F

V

V

L

P

D

Q

Q
|
Q

F

F

S

E

N

N

P

P

D

A

N

N

P

P

L

A

S

I

H

H

Y

R

E

V

G

T

T

T

G

A

P

E

E

E

I

V

W

W

R

R

D

D

T

T

R

D

G

G

G

K

I

V

T

D

H

I

F

V

V

V

S

A

A

G

M
x
V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

I

M

T

G

G

T

V

S

A

R

Q

Y

V

L

I

K

K

E

E

Q

R

D

K

P

P

S

S

V

A

E

R

V

F

V

V

G

A

V

V

T

E

P

P

A

A

E

A

G

S

A

P

S

V

I

L

P

S

G

G

I

G

R

Q

R

K

W

G

P

P

E

H

-

P

-

I

-

Q

-
x
G

-
x
I

-

G

-

A

A

G

Y

F

L

V

P

P

G

P

I

V

F

L

E

D

D

Q

F

D

R

L

V

V

D

D

R

E

T

I

L

I

E

T

V

V

T

G

Q

N

P

E

E

D

A

A

E

L

D

N

T

V

M

A

R

R

L

R

L

L

A

A

A

R

R

E

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

T

A

V

A

A

L

L

R

Q

L

V

S

A

R

R

E

R

L

P

E

E

N

N

A

A

-

G

-

K

V

L

I

I

V

V

S

V

I

V

I

L

C
x
P

D
|
D

R

F

G

G

D

E

R

R

Y

Y

I

L

S

S

T

T

G

P

V

L

F

F

active site: K44 (= K43), S266 (= S257), P293 (≠ C282)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K43), T71 (= T70), S72 (= S71), N74 (= N73), T75 (= T74), Q144 (= Q142), V177 (≠ M175), G178 (= G176), T179 (= T177), G180 (≠ T178), T182 (= T180), G222 (vs. gap), I223 (vs. gap), S266 (= S257), P293 (≠ C282), D294 (= D283)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
40% identity, 98% coverage: 4:294/296 of query aligns to 11:311/329 of 8b9wA

query
sites
8b9wA

P

P

T

S

L

V

E

D

S

A

C

L

V

I

G

G

N

N

T

T

P

P

L

A

V

V

R

Y

L

L

Q

R

R

N

L

M

P

-

G

N

E

K

T

T

S

A

N

A

T

T

I

I

L

V

V

L

K

K

L

L

E

E

G

S

N

E

N

N

P

P

A

M

G

A

S

S

V

V

K
|
K

D

D

R

R

P

L

A

A

L

Y

G

A

M

I

I

Y

R

D

K

K

A

A

E

E

E

K

R

E

G

G

D

K

I

I

R

K

P

R

G

G

D

K

T

S

-

V

L

I

V

V

E

E

A

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

L

V

L

A

G

A

A

M

V

K

R

G

G

Y

Y

R

K

M

V

V

I

L

I

I

T

M

M

P

P

E

D

N

S

M

M

S

S

A

M

E

E

R

R

A

S

S

L

M

L

K

R

A

I

F

F

G

G

A

A

E

E

I

L

I

I

L

L

V

T

S

P

R

A

E

S

Q

L

G

G

M

M

E

K

G

G

A

A

R

V

D

A

L

M

A

A

K

N

Q

R

M

I

E

V

A

S

E

N

G

N

R

P

G

D

R

A

V

V

L

L

-

A

D

D

Q

Q

F

F

S

C

N

T

P

K

D

Y

N

N

P

A

L

Q

S

I

H

H

Y

E

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

R

K

G

G

G

H

I

V

T

D

H

C

F

F

V

V

S

A

A

G

M

V

G
|
G

T
|
T

T
x
G

G

G

T
|
T

I

L

M

T

G

G

T

V

S

A

R

R

Y

F

L

L

K

K

E

S

Q

V

D

G

P

C

S

N

V

A

E

N

V

I

V

V

G

A

V

V

T

E

P

P

A

Q

E

E

G

S

A

P

S

V

I

L

P

S

G

G

I

G

R

R

R

P

W

G

P

P

E

H

-

R

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

L

V

P

P

G

E

I

V

F

L

E

D

D

R

F

E

R

L

V

V

D

D

R

E

T

I

L

I

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Q

V

V

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S

Q

S

P

D

E

K

A

A

E

I

D

E

T

T

M

A

R

Q

L

K

L

L

A

S

A

R

R

M

E

D

G

G

I

I

F

F

C

C

G

G

V

F

S
|
S

S

A

G

G

G

A

A

N

V

V

A

F

A

A

L

L

R

K

L

I

S

A

R

E

-

R

-

P

E

E

L

M

E

A

N

G

A

K

V

T

I

I

V

V

S

T

I

V

I

I

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

I

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S

S

T

T

G

A

V

L

F

Y

binding pyridoxal-5'-phosphate: K49 (= K43), N80 (= N73), G184 (= G176), T185 (= T177), G186 (≠ T178), T188 (= T180), G228 (vs. gap), S272 (= S257), P299 (≠ C282)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
43% identity, 96% coverage: 10:294/296 of query aligns to 83:378/392 of P47999

query
sites
P47999

V

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

Q

N

R

N

L

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P

V

G

K

E

G

T

C

S

V

N

A

T

S

I

V

L

A

V

A

K

K

L

L

E

E

G

I

N

M

N

E

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P

A

C

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C

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S

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V

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K

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D

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I

A

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Y

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K

D

A

A

E

E

R

K

G

G

D

L

I

I

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T

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P

G

G

D

K

T

S

-

V

L

L

V

V

E

E

A

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

V

I

A

A

M

S

K

K

G

G

Y

Y

R

K

M

L

V

I

L

L

I

T

M

M

P

P

E

A

N

S

M

M

S

S

A

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A

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S

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M

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K

R

A

A

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F

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G

A

A

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E

I

L

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V

L

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S

E

R

P

E

A

Q

K

G

G

M

M

E

T

G

G

A

A

R

I

D

Q

L

K

A

A

K

E

Q

E

-

I

M

L

E

K

A

K

E

T

G

P

R

N

G

S

R

Y

V

M

L

L

D

Q

Q

Q

F

F

S

D

N

N

P

P

D

A

N

N

P

P

L

K

S

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

E

D

D

T

T

R

R

G

G

G

K

I

I

T

D

H

I

F

L

V

V

S

A

A

G

M

I

G

G

T

T

T

G

G

G

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T

I

I

M

T

G

G

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V

S

G

R

R

Y

F

L

I

K

K

E

E

Q

R

D

K

P

P

S

E

V

L

E

K

V

V

V

I

G

G

V

V

T

E

P

P

A

T

E

E

G

S

A

A

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

E

H

-

K

-

I

-

Q

-

G

-

I

-

G

-

A

A

G

Y

F

L

V

P

P

G

K

I

N

F

L

E

D

D

L

F

A

R

I

V

V

D

D

R

E

T

Y

L

I

E

A

V

I

T

S

Q

S

P

E

E

E

A

A

E

I

D

E

T

T

M

S

R

K

L

Q

L

L

A

A

A

L

R

Q

E

E

G

G

I

L

F

L

C

V

G

G

V

I

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

Q

L

V

S

A

R

K

E

R

L

P

E

E

N

N

A

A

-

G

-

K

V

L

I

I

V

A

S

V

I

V

I

F

C

P

D

S

R

F

G

G

D

E

R

R

Y

Y

I

L

S

S

T

T

G

Q

V

L

F

F

Sites not aligning to the query:

61 modified: N-acetylalanine

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
41% identity, 98% coverage: 5:294/296 of query aligns to 16:316/323 of 4aecA

query
sites
4aecA

T

N

L

V

E

S

S

Q

C

L

V

I

G

G

N

K

T

T

P

P

L

M

V

V

R

Y

L

L

Q

N

R

S

L

I

P

A

G

K

E

G

T

C

S

V

N

A

T

N

I

I

L

A

V

A

K

K

L

L

E

E

G

I

N

M

N

E

P

P

A

C

G

C

S

S

V

V

K
|
K

D

D

R

R

P

I

A

G

L

Y

G

S

M

M

I

V

R

T

K

D

A

A

E

E

E

Q

R

K

G

G

D

F

I

I

R

S

P

P

G

G

D

K

T

S

-

V

L

L

V

V

E

E

A

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

V

I

A

A

M

S

K

R

G

G

Y

Y

R

R

M

L

V

I

L

L

I

T

M

M

P

P

E

A

N

S

M

M

S

S

A

M

E

E

R

R

A

V

S

L

M

L

K

K

A

A

F

F

G

G

A

A

E

E

I

L

I

V

L

L

V

T

S

D

R

P

E

A

Q

K

G

G

M

M

E

T

G

G

A

A

R

V

D

Q

L

K

A

A

K

E

Q

E

-

I

M

L

E

K

A

N

E

T

G

P

R

D

G

A

R

Y

V

M

L

L

D

Q

Q

Q

F

F

S

D

N

N

P

P

D

A

N

N

P

P

L

K

S

I

H

H

Y

Y

E

E

G

T

T

T

G

G

P

P

E

E

I

I

W

W

R

D

D

D

T

T

R

K

G

G

G

K

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D

H

I

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F

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V

S

A

A

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M
x
I

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|
G

T
|
T

T
x
G

G
|
G

T
|
T

I

I

M

T

G

G

T

V

S

G

R

R

Y

F

L

I

K

K

E

E

Q

K

D

N

P

P

S

K

V

T

E

Q

V

V

V

I

G

G

V

V

T

E

P

P

A

T

E

E

G

S

A

D

S

I

I

L

P

S

G

G

I

G

R

K

R

P

W

G

P

P

E

H

-

K

-

I

-

Q

-
x
G

-

I

-

G

-

A

A

G

Y

F

L

I

P

P

G

K

I

N

F

L

E

D

D

Q

F

K

R

I

V

M

D

D

R

E

T

V

L

I

E

A

V

I

T

S

Q

S

P

E

E

E

A

A

E

I

D

E

T

T

M

A

R

K

L

Q

L

L

A

A

A

L

R

K

E

E

G

G

I

L

F

M

C

V

G

G

V

I

S
|
S

S

S

G

G

G

A

A

A

V

A

A

A

L

I

R

K

L

V

S

A

R

K

E

R

L

P

E

E

N

N

A

A

-

G

-

K

V

L

I

I

V

A

S

V

I

V

I

F

C
x
P

D

S

R

F

G

G

D

E

R

R

Y

Y

I

L

S

S

T

T

G

P

V

L

F

F

active site: K54 (= K43), S277 (= S257)
binding pyridoxal-5'-phosphate: K54 (= K43), N85 (= N73), I188 (≠ M175), G189 (= G176), T190 (= T177), G191 (≠ T178), G192 (= G179), T193 (= T180), G233 (vs. gap), S277 (= S257), P304 (≠ C282)

Query Sequence

>WP_018231161.1 NCBI__GCF_000378965.1:WP_018231161.1
MTYPTLESCVGNTPLVRLQRLPGETSNTILVKLEGNNPAGSVKDRPALGMIRKAEERGDI
RPGDTLVEATSGNTGIALAMVAAMKGYRMVLIMPENMSAERRASMKAFGAEIILVSREQG
MEGARDLAKQMEAEGRGRVLDQFSNPDNPLSHYEGTGPEIWRDTRGGITHFVSAMGTTGT
IMGTSRYLKEQDPSVEVVGVTPAEGASIPGIRRWPEAYLPGIFEDFRVDRTLEVTQPEAE
DTMRLLAAREGIFCGVSSGGAVAAALRLSRELENAVIVSIICDRGDRYISTGVFPA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory