SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_018231339.1 NCBI__GCF_000378965.1:WP_018231339.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 19 hits to proteins with known functional sites (download)

Q5F8J4 NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
56% identity, 100% coverage: 1:435/436 of query aligns to 1:433/435 of Q5F8J4

query
sites
Q5F8J4

M

M

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K

P

P

V

V

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N

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I

G

G

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C

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G

A

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L45 (≠ R45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ RD 45:46) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
40% identity, 99% coverage: 2:433/436 of query aligns to 3:429/431 of 6dzsA

query
sites
6dzsA

K

K

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P

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A

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G
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G

T
x
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A
x
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x
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), L12 (= L11), G13 (= G12), N14 (≠ T13), V15 (= V14), V45 (= V39), R46 (≠ V40), R47 (≠ A41), R52 (≠ L47), I63 (= I58), L78 (= L79), M79 (≠ I80), P84 (= P85), A102 (= A103), K104 (= K105), G306 (= G302), T310 (= T306)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
39% identity, 77% coverage: 1:337/436 of query aligns to 2:323/402 of 4pg7A

query
sites
4pg7A

M

M

K

K

P

K

V

L

N

N

V

I

G

A

L

L

G

G

L

L

G

G

T

T

V

V

G

G

C

S

G

G

A

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K

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I

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E

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E

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N

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D

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E

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A

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A

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F

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D

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R

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Y

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G

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D

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A

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D

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Y

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G

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A

G

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S

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A

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D

D

L

L

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L

D

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V

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A

F

L

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S

D

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D

active site: D190 (= D201), K194 (= K205)
binding lysine: R215 (≠ E226), G219 (≠ R230)

Sites not aligning to the query:

binding lysine: 394, 395

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
38% identity, 67% coverage: 1:292/436 of query aligns to 1:280/332 of 6a0tB

query
sites
6a0tB

M

M

K

E

P

A

V

L

N

K

V

I

G

A

L

L

G
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G

L
x
G

G
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G

T
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T

V
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V

G

G

C

S

G

A

A

F

I

Y

N

N

V

L

L

V

A

L

R

E

N

R

G

A

D

E

E

E

I

L

A

S

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E

R

V

F

V

L

A

G

A

V

S
x
L

A
x
V

R
|
R

D
|
D

L

P

E

R

R
x
K

P

P

R
|
R

E

A

C

I

S

P

L

Q

D

E

R

L

I

L

R

R

L

-

H

-

A

A

D

E

P

P

F

F

D

D

V

L

N

-

D

-

P

-

Q

E

V

A

Q

D

I

L

V

V

E

E

L
x
A

I
x
M

G
|
G

G

G

T

V

S

E

P

A

A

P

R

L

E

R

L

L

V

V

L

L

A

P

A

A

I

L

E

E

Q

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

I

L

A

A

L

E

H

A

G

W

N

E

E

S

I

L

F

R

A

P

A

F

A

A

Q

E

A

E

R

G

G

-

V

-

M

L

V

I

A

Y

F

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

K

S

A

F

I

L

R

-

E

E

G

T

L

L

S

R

G

G

N

S

R

E

I

L

L

L

W

E

L

L

A

H

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

G

T

N

L

F

Y

I

I

L

L

T

Q

E

E

M

M

R

-

D

E

K

K

G

G

R

R

E

T

F

Y

A

A

D

E

V

A

L

L

A

L

E

E

A

A

Q

Q

R

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

E

E

G

G

I

I

D
|
D

A

A

H

H

K
|
K

L

L

T

T

I

L

L

L

A

A

S

R

I

L

A

L

F

V

G

D

I

P

P

G

L

F

Q

P

F

F

D

A

K

E

V

V

Y

E

T

A

E

Q

G

G

I

I

A

A

R

R

I

L

T

T

R

P

E

E

D

V

V

L

A

Q

Y

K

A

A

A

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

H

L

L

-

G

-

I

V

A

A

R

S

Q

L

S

F

E

G

Q

E

G

G

I

G

E

R

L

W

R

R

-

A

V

V

H

A

P

P

T

R

L

R

I

L

P

P

H

Q

R

D

R

H

L

P

I

L

A

A

N

R

V

A

D

R

G

G

V

-

M

-

N

N

A

A

V

L

L

W

V

V

M

R

G

A

D

R

A

P

V

L

G

G

active site: D191 (= D201), K195 (= K205)
binding l-homoserine: N150 (= N159), G151 (= G160), T152 (= T161), Y178 (= Y188), E180 (= E190), D186 (= D196), K195 (= K205)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (≠ S43), V43 (≠ A44), R44 (= R45), D45 (= D46), K48 (≠ R49), R50 (= R51), A73 (≠ L79), M74 (≠ I80), G75 (= G81), A97 (= A103), N98 (= N104), G177 (= G187), E180 (= E190)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
38% identity, 67% coverage: 1:292/436 of query aligns to 1:280/331 of 6a0sA

query
sites
6a0sA

M

M

K

E

P

A

V

L

N

K

V

I

G

A

L

L

G

G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

C

S

G

A

A

F

I

Y

N

N

V

L

L

V

A

L

R

E

N

R

G

A

D

E

E

E

I

L

A

S

R

A

R

-

A

F

G

G

R

V

G

V

I

P

E

R

V

F

V

L

A

G

A

V

S
x
L

A
x
V

R
|
R

D
|
D

L

P

E

R

R
x
K

P

P

R
|
R

E

A

C

I

S

P

L

Q

D

E

R

L

I

L

R

R

L

-

H

-

A

A

D

E

P

P

F

F

D

D

V

L

N

-

D

-

P

-

Q

E

V

A

Q

D

I

L

V

V

E

E

L
x
A

I
x
M

G

G

T

V

S

E

P

A

A

P

R

L

E

R

L

L

V

V

L

L

A

P

A

A

I

L

E

E

Q

A

G

G

K

I

H

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

I

L

A

A

L

E

H

A

G

W

N

E

E

S

I

L

F

R

A

P

A

F

A

A

Q

E

A

E

R

G

G

-

V

-

M

L

V

I

A

Y

F

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

I

L

K

S

A

F

I

L

R

-

E

E

G

T

L

L

S

R

G

G

N

S

R

E

I

L

L

L

W

E

L

L

A

H

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

G

T

N

L

F

Y

I

I

L

L

T

Q

E

E

M

M

R

-

D

E

K

K

G

G

R

R

E

T

F

Y

A

A

D

E

V

A

L

L

A

L

E

E

A

A

Q

Q

R

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

V

V

E

E

G

G

I

I

D
|
D

A

A

H

H

K
|
K

L

L

T

T

I

L

L

L

A

A

S

R

I

L

A

L

F

V

G

D

I

P

P

G

L

F

Q

P

F

F

D

A

K

E

V

V

Y

E

T

A

E

Q

G

G

I

I

A

A

R

R

I

L

T

T

R

P

E

E

D

V

V

L

A

Q

Y

K

A

A

A

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

H

L

L

-

G

-

I

V

A

A

R

S

Q

L

S

F

E

G

Q

E

G

G

I

G

E

R

L

W

R

R

-

A

V

V

H

A

P

P

T

R

L

R

I

L

P

P

H

Q

R

D

R

H

L

P

I

L

A

A

N

R

V

A

D

R

G

G

V

-

M

-

N

N

A

A

V

L

L

W

V

V

M

R

G

A

D

R

A

P

V

L

G

G

active site: D191 (= D201), K195 (= K205)
binding l-homoserine: K99 (= K105), N150 (= N159), G151 (= G160), T152 (= T161), Y178 (= Y188), E180 (= E190), D186 (= D196), K195 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (≠ S43), V43 (≠ A44), R44 (= R45), D45 (= D46), K48 (≠ R49), R50 (= R51), A73 (≠ L79), M74 (≠ I80), A97 (= A103), K99 (= K105), G177 (= G187), E180 (= E190)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
38% identity, 67% coverage: 1:292/436 of query aligns to 1:280/331 of 2ejwA

query
sites
2ejwA

M

M

K

E

P

A

V

L

N

K

V

I

G

A

L

L

G

G

L

G

G

G

T

T

V

V

G

G

C

S

G

A

A

F

I

Y

N

N

V

L

L

V

A

L

R

E

N

R

G

A

D

E

E

E

I

L

A

S

R

A

R

-

A

F

G

G

R

V

G

V

I

P

E

R

V

F

V

L

A

G

A

V

S

L

A

V

R

R

D

D

L

P

E

R

R

K

P

P

R

R

E

A

C

I

S

P

L

Q

D

E

R

L

I

L

R

R

L

-

H

-

A

A

D

E

P

P

F

F

D

D

V

L

N

-

D

-

P

-

Q

E

V

A

Q

D

I

L

V

V

E

E

L

A

I

M

G

G

T

V

S

E

P

A

A

P

R

L

E

R

L

L

V

V

L

L

A

P

A

A

I

L

E

E

Q

A

G

G

K

I

H

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

I

L

A

A

L

E

H

A

G

W

N

E

E

S

I

L

F

R

A

P

A

F

A

A

Q

E

A

E

R

G

G

-

V

-

M

L

V

I

A

Y

F

H

E
|
E

A

A

A

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

I

A

I

L

K

S

A

F

I

L

R

-

E

E

G

T

L

L

S

R

G

G

N

S

R

E

I

L

L

L

W

E

L

L

A

H

G

G

I

I

I

L

N

N

G

G

T

T

G

T

N

L

F

Y

I

I

L

L

T

Q

E

E

M

M

R

-

D

E

K

K

G

G

R

R

E

T

F

Y

A

A

D

E

V

A

L

L

A

L

E

E

A

A

Q

Q

R

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

T

T

F

L

D

D

V

V

E

E

G

G

I

I

D
|
D

A

A

H

H

K
|
K

L

L

T

T

I

L

L

L

A

A

S

R

I

L

A

L

F

V

G

D

I

P

P

G

L

F

Q

P

F

F

D

A

K

E

V

V

Y

E

T

A

E

Q

G

G

I

I

A

A

R

R

I

L

T

T

R

P

E

E

D

V

V

L

A

Q

Y

K

A

A

A

E

E

A

L

R

G

G

Y

E

R

R

I

V

K

R

H

L

L

-

G

-

I

V

A

A

R

S

Q

L

S

F

E

G

Q

E

G

G

I

G

E

R

L

W

R

R

-

A

V

V

H

A

P

P

T

R

L

R

I

L

P

P

H

Q

R

D

R

H

L

P

I

L

A

A

N

R

V

A

D

R

G

G

V

-

M

-

N

N

A

A

V

L

L

W

V

V

M

R

G

A

D

R

A

P

V

L

G

G

active site: D191 (= D201), K195 (= K205)
binding magnesium ion: E121 (= E129), V124 (= V132), M125 (≠ A133), A126 (≠ G134), T128 (≠ I136)

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
37% identity, 60% coverage: 60:319/436 of query aligns to 53:297/300 of 7f4cA

query
sites
7f4cA

L

L

H

E

A

G

D

D

P

V

F

F

D

K

V

A

V

F

N

E

D

K

P

I

Q

K

V

P

Q

D

I

I

V

I

V

V

E

D

L
x
V

I
x
S

G
x
S

G

A

T

N

-

Y

-

N

-

G

S

E

P

P

A

S

R

L

E

S

L

L

V

Y

L

K

A

E

A

A

I

I

E

K

Q

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

I

L

A

A

L

L

H

A

G

F

N

N

E

E

I

I

F

F

A

S

A

L

A

A

Q

R

A

S

R

K

G

G

V

V

M

K

V

I

A

G

F

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

K

N

A

L

I

Y

R

R

E

V

G

-

L

L

S

P

G

G

N

S

R

R

I

V

L

I

W

K

L

I

A

R

G

G

I

I

I

L

N

N

G

G

T

T

G

T

N

N

F

F

I

I

L

L

T

T

E

L

M

M

R

-

D

N

K

K

G

G

R

V

E

S

F

F

A

E

D

E

V

A

L

L

A

K

E

E

A

A

Q

Q

R

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

V

I

E

N

G

G

I

F

D

D

A

A

H

A

K

K

L

I

T

T

I

I

L

L

A

A

S

N

I

F

A

M

F

I

G

G

I

N

P

S

L

V

Q

T

F

I

D

K

K

D

V

V

Y

K

T

F

E

E

G

G

I

I

A

N

R

R

I

D

T

L

R

K

-

I

E

K

D

L

V

I

A

A

Y

Y

A

A

A

D

E

E

L

K

G

-

Y

-

R

-

I

-

K

-

H

-

L

-

G

-

I

-

A

-

R

-

Q

-

S

-

E

-

Q

-

G

-

I

-

E

E

L

V

R

W

V

V

H

K

P

P

T

L

L

P

I

I

P

S

H

Q

R

D

L

P

I

L

A

Y

N

N

V

V

D

D

G

G

V

V

M

E

N

N

A

A

V

L

L

E

V

I

M

T

G

T

D

D

A

-

V

I

G

Q

P

S

T

I

L

L

Y

I

Y

R

G

G

A

P

G

G

A

A

G
|
G

A

P

E

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

L

L

V

I

D

L

V

L

V

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (≠ L79), S73 (≠ I80), S74 (≠ G81), T100 (≠ A103), K102 (= K105), G127 (≠ A130), S131 (≠ G134), E185 (= E190), G280 (= G302), A284 (≠ T306)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 6, 7, 8, 9, 10, 11, 37, 38, 39

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
37% identity, 60% coverage: 60:319/436 of query aligns to 53:299/302 of 5x9dA

query
sites
5x9dA

L

L

H

E

A

G

D

D

P

V

F

F

D

K

V

A

V

F

N

E

D

K

P

I

Q

K

V

P

Q

D

I

I

V

I

V

V

E

D

L
x
V

-
x
S

-

A

-

N

I

Y

G

N

T

G

S

E

P
|
P

A

S

R

L

E

S

L

L

V

Y

L

K

A

E

A

A

I

I

E

K

Q

D

G

G

K

V

H

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

I

L

A

A

L

L

H

A

G

F

N

N

E

E

I

I

F

F

A

S

A

L

A

A

Q

R

A

S

R

K

G

G

V

V

M

K

V

I

A

G

F

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

I

I

K

N

A

L

I

Y

R

R

E

V

G

-

L

L

S

P

G

G

N

S

R

R

I

V

L

I

W

K

L

I

A

R

G

G

I

I

I

L

N
|
N

G
|
G

T
|
T

G

T

N

N

F

F

I

I

L

L

T

T

E

L

M

M

R

-

D

N

K

K

G

G

R

V

E

S

F

F

A

E

D

E

V

A

L

L

A

K

E

E

A

A

Q

Q

R

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

F

L

D
|
D

V

I

E

N

G

G

I

F

D
|
D

A

A

H

A

K
|
K

L

I

T

T

I

I

L

L

A

A

S

N

I

F

A

M

F

I

G

G

I

N

P

S

L

V

Q

T

F

I

D

K

K

D

V

V

Y

K

T

F

E

E

G

G

I

I

A

N

R

R

I

D

T

P

R

K

E

N

D

E

-

K

-

I

-

K

-

L

V

I

A

A

Y

Y

A

A

A

D

E

E

L

K

G

E

Y

V

R

W

I

V

K

K

H

P

L

L

G

P

I

I

A

S

R

Q

Q

D

S

P

E

-

Q

-

G

-

I

-

E

-

L

-

R

-

V

-

H

-

P

-

T

-

L

-

I

-

P

-

H

-

R

-

L

-

I

L

A

Y

N

N

V

V

D

D

G

G

V

V

M

E

N

N

A

A

V

L

L

E

V

I

M

T

G

T

D

D

A

-

V

I

G

Q

P

S

T

I

L

L

Y

I

Y

R

G

G

A

P

G

G

A
|
A

G
|
G

A

P

E

V

P

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

A

S

D

D

L

L

V

I

D

L

V

L

V

K

R

R

active site: D196 (= D201), K200 (= K205)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: V72 (≠ L79), S73 (vs. gap), S74 (vs. gap), P82 (= P85), T100 (≠ A103), N101 (= N104), K102 (= K105), G127 (≠ A130), S131 (≠ G134), N155 (= N159), G156 (= G160), T157 (= T161), Y183 (= Y188), A184 (= A189), E185 (= E190), D191 (= D196), D196 (= D201), K200 (= K205), A281 (= A301), G282 (= G302), A286 (≠ T306)

Sites not aligning to the query:

binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: 6, 7, 8, 9, 10, 11, 37, 38

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
28% identity, 72% coverage: 4:315/436 of query aligns to 3:309/319 of 4xb2A

query
sites
4xb2A

V

V

N

N

V

I

G

S

L

I

L
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

C

R

G

A

A

L

I

A

N

E

V

I

L

I

A

A

R

-

N

-

G

-

D

-

E

E

I

K

A

S

R

R

R

I

A

F

G

G

R

V

G

E

I

L

E

N

V

V

V

I

A

S

A

I

S

T

A

D

R
|
R

-

S

-

G

-

T

-

I

-

W

-

G

-

D

-

F

D

D

L

L

E

L

R

E

P

A

R

K

E

E

C

V

S

A

L

E

D

S

R

T

I

G

R

K

L

L

-

S

-

N

-

I

-

G

-

D

-

Y

-

E

-

V

-

Y

H

N

A

F

D

S

P

P

F

Q

D

E

V

L

V

V

N

E

D

E

P

V

Q

K

V

P

Q

N

I

I

V

L

V

V

E

D

L

-

I
x
V

G
x
S

T

W

S

D

P

E

A

A

R

H

E

E

L

M

V

Y

L

K

A

V

A

A

I

L

E

G

Q

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

I

I

A

A

L

N

H

Y

G

Y

N

D

E

E

I

L

F

M

A

N

A

L

A

A

Q

K

A

E

R

N

G

N

V

A

M

G

V

I

A

F

F

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

K

G

A

V

I

L

R

R

E

E

G

N

L

L

S

L

G

G

N

E

R

N

I

I

L

K

W

R

L

I

A

D

G

A

I

V

N

N

G
x
A

T
x
S

G

T

N

T

F

F

I

I

L

L

T

T

E

K

M

M

R

S

D

E

K

-

G

G

R

K

E

T

F

L

A

D

D

D

V

A

L

I

A

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D
|
D

V

I

E

D

G

G

I

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

T

K

I

I

L

L

A

H

S

W

I

V

A

S

F

Y

G

G

I

E

P

P

L

P

Q

E

F

E

D

E

K

E

V

-

Y

-

T

-

E

-

G

-

I

-

A

-

R

R

I

L

T

G

R

I

E

R

D

E

V

V

A

R

Y

D

A

A

A

-

E

-

L

-

G

-

Y

-

R

-

I

-

K

R

H

N

L

V

G

R

I

L

A

V

R

A

Q

Q

S

V

E

S

Q

K

G

G

I

-

E

K

L

I

R

S

V

V

H

K

P

P

T

-

L

-

I

-

P

-

H

-

R

R

R

K

L

L

I

S

A

S

N

D

-

N

-

P

-

L

V

V

D

E

G

G

V

V

M

Q

N

N

A

A

V

A

L

V

V

I

M

R

G

T

D

N

A

N

V

L

G

G

P

E

T

V

L

I

Y

L

Y

K

G

G

A

P

G

G

A
x
G

G

G

A

G

E

R

P

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

L

I

V

I

active site: D211 (= D201), K215 (= K205)
binding l-homoserine: A171 (≠ G160), S172 (≠ T161), D206 (= D196), K215 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L9), F10 (≠ L11), G11 (= G12), T12 (= T13), V13 (= V14), R40 (= R45), V91 (≠ I80), S92 (≠ G81), S93 (≠ G82), S114 (≠ A103), N115 (= N104), K116 (= K105), S141 (≠ A130), G295 (≠ A301), T300 (= T306)

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
28% identity, 72% coverage: 4:315/436 of query aligns to 3:309/319 of 4xb1A

query
sites
4xb1A

V

V

N

N

V

I

G

S

L

I

L
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

C

R

G

A

A

L

I

A

N

E

V

I

L

I

A

A

R

-

N

-

G

-

D

-

E

E

I

K

A

S

R

R

R

I

A

F

G

G

R

V

G

E

I

L

E

N

V

V

V

I

A

S

A

I

S

T

A
x
D

R
|
R

-

S

-

G

-

T

-

I

-

W

-

G

-

D

-

F

D

D

L

L

E

L

R

E

P

A

R

K

E

E

C

V

S
x
K

L

E

D

S

R

T

I

G

R

K

L

L

-

S

-

N

-

I

-

G

-

D

-

Y

-

E

-

V

-

Y

H

N

A

F

D

S

P

P

F

Q

D

E

V

L

V

V

N

E

D

E

P

V

Q

K

V

P

Q

N

I

I

V

L

V

V

E

D

L

-

I
x
V

G
x
S

T

W

S

D

P

E

A

A

R

H

E

E

L

M

V

Y

L

K

A

V

A

A

I

L

E

G

Q

E

G

G

K

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

I

I

A

A

L

N

H

Y

G

Y

N

D

E

E

I

L

F

M

A

N

A

L

A

A

Q

K

A

E

R

N

G

N

V

A

M

G

V

I

A

F

F

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

K

G

A

V

I

L

R

R

E

E

G

N

L

L

S

L

G

G

N

E

R

N

I

I

L

K

W

R

L

I

A

D

G

A

I

V

N

N

G

A

T

S

G

T

N

T

F

F

I

I

L

L

T

T

E

K

M

M

R

S

D

E

K

-

G

G

R

K

E

T

F

L

A

D

D

D

V

A

L

I

A

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

V

I

E

D

G

G

I

I

D
|
D

A

A

A

Y

H

Y

K
|
K

L

A

T

K

I

I

L

L

A

H

S

W

I

V

A

S

F

Y

G

G

I

E

P

P

L

P

Q

E

F

E

D

E

K

E

V

-

Y

-

T

-

E

-

G

-

I

-

A

-

R

R

I

L

T

G

R

I

E

R

D

E

V

V

A

R

Y

D

A

A

A

-

E

-

L

-

G

-

Y

-

R

-

I

-

K

R

H

N

L

V

G

R

I

L

A

V

R

A

Q

Q

S

V

E

S

Q

K

G

G

I

-

E

K

L

I

R

S

V

V

H

K

P

P

T

-

L

-

I

-

P

-

H

-

R

R

R

K

L

L

I

S

A

S

N

D

-

N

-

P

-

L

V

V

D

E

G

G

V

V

M

Q

N

N

A

A

V

A

L

V

V

I

M

R

G

T

D

N

A

N

V

L

G

G

P

E

T

V

L

I

Y

L

Y

K

G

G

A

P

G

G

A
x
G

G

G

A

G

E

R

P

V

T
|
T

A

A

S

S

A

G

V

V

V

F

A

T

D

D

L

I

V

I

active site: D211 (= D201), K215 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L9), F10 (≠ L11), G11 (= G12), T12 (= T13), V13 (= V14), D39 (≠ A44), R40 (= R45), K57 (≠ S54), V91 (≠ I80), S92 (≠ G81), S93 (≠ G82), S114 (≠ A103), K116 (= K105), S141 (≠ A130), G295 (≠ A301), T300 (= T306)

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
31% identity, 52% coverage: 1:228/436 of query aligns to 1:239/321 of 3jsaA

query
sites
3jsaA

M

M

K

K

P

T

V

I

N

N

V

L

G

S

L

I

L

F

G
|
G

L
|
L

G
|
G

T
x
N

V
|
V

G

G

C

L

G

N

A

L

I

L

N

R

V

I

L

I

A

R

R

S

N

F

G

N

D

E

E

E

I

-

A

-

R

N

R

R

A

L

G

G

R

L

G

K

I

F

E

N

V

V

-

F

-

V

A

A

A
x
D

S
|
S

A

L

R

H

D

S

L

Y

E

Y

R

N

P

E

R

R

-

I

-

D

-

I

-

G

-

K

-

V

-

I

-

S

-

Y

-

K

-

E

E

K

C

G

S

S

L

L

D

D

R

S

I

L

R

E

L

Y

H

E

-

S

-

I

-

S

-

A

-

S

-

E

-

A

-

L

A

A

D

R

P

D

F

F

D

D

V

I

V

V

N

V

D

D

P
x
A

Q

-

V

-

Q

-

I

-

V

-

E

-

L

-

I

-

G

-

T

-

S
x
T

P
|
P

A

A

-

S

-

A

-

D

-

G

-

K

R

K

E

E

L

L

V

A

L

F

A

Y

-

K

-

E

A

T

I

F

E

E

Q

N

G

G

K

K

H

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

I

L

A

A

L

N

H

F

G

W

N

P

E

E

I

I

F

M

A

E

A

Y

A

A

Q

R

A

S

R

N

G

N

V

R

M

R

V

I

A

R

F

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

K

S

A

F

I

I

R

D

E

Y

G

S

L

V

S

L

G

P

N

S

R

R

I

I

L

K

W

K

L

F

A

R

G

G

I

I

I

V

N

S

G

L

T

T

G

I

N

N

F

Y

I

F

L

I

T

R

E

E

M

L

R

A

D

N

K

K

G

-

R

R

E

E

F

F

A

D

D

D

V

V

L

L

A

S

E

E

A

A

Q

T

R

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

T

-

F

-

D

D

V

L

E

T

G

G

I

L

D
|
D

A

A

H

R

K
|
K

L

S

T

V

I

I

L

L

A

C

S

N

I

H

A

L

F

Y

G

G

I

S

P

S

L

Y

Q

R

F

L

D

S

K

D

V

V

Y

F

T

Y

E

E

G

G

I

I

active site: D212 (= D201), K216 (= K205)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), L11 (= L11), G12 (= G12), N13 (≠ T13), V14 (= V14), D42 (≠ A42), S43 (= S43), A90 (≠ P71), T91 (≠ S84), P92 (= P85), A117 (= A103), N118 (= N104), A144 (= A130)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 308

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
28% identity, 52% coverage: 2:228/436 of query aligns to 1:236/319 of 3ingA

query
sites
3ingA

K

K

P

E

V

I

N

R

V

I

G

I

L

L

L

M

G
|
G

L
x
T

G
|
G

T
x
N

V
|
V

G

G

C

L

G

N

A

V

I

L

N

R

V

I

L

I

A

-

R

-

N

-

G

-

D

-

E

D

I

A

A

S

R

N

R

R

A

R

G

R

R

F

G

S

I

I

E

K

V

V

A

G

A

V

S

S

-
x
D

A
x
S

R

R

D

S

L

Y

E

A

R

S

P

G

R

R

E

N

C

L

S

D

L

I

D

S

R

S

I

I

R

I

L

S

H

N

A
x
K

D

E

P

K

F

T

D

G

V

R

V

I

N

S

D

D

P

R

Q

A

V

F

Q

S

I

G

V

P

V

E

E

D

L

L

I

M

G

G

-

E

-

A

-

D

-

L

-

V

G

D

T
x
C

S
x
T

P
|
P

A

A

-

S

-

R

-

D

-

G

-

V

R

R

E

E

L

Y

V

S

L

L

-

Y

-

R

A

M

A

A

I

F

E

E

Q

S

G

G

K

M

H

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

I

L

A

A

L

N

H

K

G

W

N

H

E

D

I

I

F

M

A

D

A

S

A

A

Q

N

A

Q

R

N

G

S

V

K

M

Y

V

I

A

R

F

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

I

L

I

F

K

S

A

V

I

L

R

D

E

Y

G

S

L

I

S

L

G

P

N

S

R

K

I

V

L

K

W

R

L

F

A

R

G

G

I

I

I

V

N

S

G

S

T

T

G

I

N

N

F

Y

I

V

L

I

T

R

E

N

M

M

R

A

D

N

K

-

G

G

R

R

E

S

F

L

A

R

D

D

V

V

L

V

A

D

E

D

A

A

Q

I

R

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

A

H

R

K
|
K

L

S

T

V

I

I

L

L

A

V

S

N

I

H

A

I

F

F

G

G

I

T

P

E

L

Y

Q

T

F

L

D

N

K

D

V

V

Y

E

T

Y

E

S

G

G

I

V

active site: D209 (= D201), K213 (= K205)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), T10 (≠ L11), G11 (= G12), N12 (≠ T13), V13 (= V14), D38 (vs. gap), S39 (≠ A44), K57 (≠ A62), C85 (≠ T83), T86 (≠ S84), P87 (= P85), A112 (= A103), N113 (= N104), K114 (= K105), A139 (= A130), E198 (= E190), S199 (≠ A191)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

O94671 Probable homoserine dehydrogenase; HDH; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 26% coverage: 99:213/436 of query aligns to 117:239/376 of O94671

query
sites
O94671

H

N

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

I

F

A

S

L

A

H

S

G

N

N

D

-

V

-

Y

-

Q

E

N

I

I

F

I

A

K

A

A

A

S

Q

K

A

E

R

S

G

G

V

A

M

L

V

L

A

M

F

H

E

E

A

A

A

S

V

V

A

G

G

A

G

G

I

L

P

P

I

I

K

S

A

T

I

L

R

K

E

E

G

L

L

I

-

A

S

T

G

G

N

D

R

E

I

I

L

I

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

V

M

W

R

S

D

P

K

N

G

G

R

K

E

K

-

G

-

T

-

A

-
x
S

F

F

A

S

D

D

V

I

L

V

A

K

E

I

A

A

Q

K

R

Q

L

N

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

M

D

D

A

V

A

A

H

R

K

K

L

V

T

T

I

I

L

L

A

S

S

R

I

I

A

A

S201 (vs. gap) modified: Phosphoserine

P31116 Homoserine dehydrogenase; HDH; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 28% coverage: 94:213/436 of query aligns to 106:231/359 of P31116

query
sites
P31116

I

V

E

E

Q

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

I

F

A

S

L

S

H

D

-

L

-

A

-

T

G

W

N

K

E

A

I

L

F

F

A

S

A

N

A

K

Q

P

A

T

R

N

G

G

V

-

M

F

V

V

A

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

S

T

G

G

N

D

R

E

I

V

L

E

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

R

S

D

T

K

S

G

Q

R

A

-

N

-

D

-

V

E

K

F

F

A

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E
|
E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

K117 (= K105) binding NADP(+)
E208 (= E190) binding substrate; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D201) mutation to L: Reduces kcat 150-fold.
K223 (= K205) mutation to V: Loss of activity.

Sites not aligning to the query:

11:18 binding NADP(+)
93 binding NADP(+)

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
33% identity, 28% coverage: 94:213/436 of query aligns to 105:230/358 of 1tveA

query
sites
1tveA

I

V

E

E

Q

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

I

F

A

S

L

S

H

D

-

L

-

A

-

T

G

W

N

K

E

A

I

L

F

F

A

S

A

N

A

K

Q

P

A

T

R

N

G

G

V

-

M

F

V

V

A

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

S

T

G

G

N

D

R

E

I

V

L

E

W

K

L

I

A

E

G

G

I

I

I

F

N

S

G

G

T
|
T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

R

S

D

T

K

S

G

Q

R

A

-

N

-

D

-

V

E

K

F

F

A

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D
|
D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

active site: D218 (= D201), K222 (= K205)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K105), T175 (= T161), D213 (= D196), D218 (= D201)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
33% identity, 28% coverage: 94:213/436 of query aligns to 105:230/358 of 1q7gA

query
sites
1q7gA

I

V

E

E

Q

N

G

G

K

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

I

F

A

S

L

S

H

D

-

L

-

A

-

T

G

W

N

K

E

A

I

L

F

F

A

S

A

N

A

K

Q

P

A

T

R

N

G

G

V

-

M

F

V

V

A

Y

F

H

E

E

A
|
A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

K

S

A

F

I

L

R

R

E

E

G

I

L

I

-

Q

S

T

G

G

N

D

R

E

I

V

L

E

W

K

L

I

A

E

G

G

I

I

I

F

N
x
S

G

G

T

T

G

L

N

S

F

Y

I

I

L

F

T

N

E

E

M

F

R

S

D

T

K

S

G

Q

R

A

-

N

-

D

-

V

E

K

F

F

A

S

D

D

V

V

L

V

A

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

V

L

E

N

G

G

I

L

D
|
D

A

V

A

A

H

R

K
|
K

L

V

T

T

I

I

L

V

A

G

S

R

I

I

A

S

active site: D218 (= D201), K222 (= K205)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A103), K116 (= K105), A143 (= A130), S173 (≠ N159), K222 (= K205)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
33% identity, 28% coverage: 94:213/436 of query aligns to 105:230/358 of 1ebuD

query
sites
1ebuD

I

V

E

E

Q

N

G

G

K

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

I

F

A

S

L

S

H

D

-

L

-

A

-

T

G

W

N

K

E

A

I

L

F

F

A

S

A

N

A

K

Q

P

A

T

R

N

G

G

V

-

M

F

V

V

A

Y

F

H

E

E