SitesBLAST
Comparing WP_018231642.1 NCBI__GCF_000378965.1:WP_018231642.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8y83A Crystal structure of a ketoreductase from sphingobacterium siyangense sy1 with co-enzyme
48% identity, 98% coverage: 3:247/250 of query aligns to 4:248/249 of 8y83A
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), S16 (= S15), G17 (= G16), I18 (= I17), D37 (= D36), I38 (≠ M37), A62 (≠ V62), D63 (= D63), S64 (≠ V64), N90 (= N90), M141 (≠ C140), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (≠ A186), Y188 (= Y187), I189 (= I188), L193 (= L192)
6ixmC Crystal structure of the ketone reductase chkred20 from the genome of chryseobacterium sp. Ca49 complexed with NAD (see paper)
46% identity, 98% coverage: 3:247/250 of query aligns to 3:247/248 of 6ixmC
- active site: G16 (= G16), S142 (= S142), Y155 (= Y155), K159 (= K159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), S15 (= S15), G16 (= G16), I17 (= I17), D36 (= D36), I37 (≠ M37), A61 (≠ V62), D62 (= D63), T63 (≠ V64), N89 (= N90), A90 (= A91), M140 (≠ C140), S142 (= S142), Y155 (= Y155), K159 (= K159), P185 (= P185), A186 (= A186), Y187 (= Y187), I188 (= I188), L192 (= L192)
4wecA Crystal structure of a short chain dehydrogenase from mycobacterium smegmatis
47% identity, 98% coverage: 2:247/250 of query aligns to 7:252/258 of 4wecA
- active site: G21 (= G16), S143 (= S142), Q154 (≠ S153), Y157 (= Y155), K161 (= K159)
- binding nicotinamide-adenine-dinucleotide: G17 (= G12), A19 (= A14), S20 (= S15), G21 (= G16), I22 (= I17), D41 (= D36), I42 (≠ M37), V61 (= V62), D62 (= D63), V63 (= V64), N89 (= N90), T141 (≠ C140), Y157 (= Y155), K161 (= K159), P187 (= P185), P189 (≠ Y187), V190 (≠ I188)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
41% identity, 99% coverage: 1:247/250 of query aligns to 3:254/255 of 5itvA
- active site: G18 (= G16), S141 (= S142), Y154 (= Y155), K158 (= K159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), S17 (= S15), G18 (= G16), I19 (= I17), D38 (= D36), I39 (≠ M37), T61 (≠ V62), I63 (≠ V64), N89 (= N90), G91 (= G92), T139 (≠ C140), S141 (= S142), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (≠ A186), I186 (≠ Y187), I187 (= I188)
5itvD Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
41% identity, 99% coverage: 1:247/250 of query aligns to 3:226/227 of 5itvD
- active site: G18 (= G16), S141 (= S142), Y154 (= Y155), K158 (= K159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), S17 (= S15), G18 (= G16), I19 (= I17), D38 (= D36), I39 (≠ M37), T61 (≠ V62), D62 (= D63), I63 (≠ V64), N89 (= N90), T139 (≠ C140), S141 (= S142), Y154 (= Y155), K158 (= K159), P184 (= P185), G185 (≠ A186), I187 (= I188)
Q9LBG2 Levodione reductase; (6R)-2,2,6-trimethyl-1,4-cyclohexanedione reductase; EC 1.1.1.- from Leifsonia aquatica (Corynebacterium aquaticum) (see paper)
43% identity, 98% coverage: 2:247/250 of query aligns to 10:265/267 of Q9LBG2
- 17:42 (vs. 9:34, 50% identical) binding NAD(+)
- E103 (vs. gap) mutation E->A,D,N,Q: 26-fold increase in Km and a much lower enantiomeric excess of the reaction products.
1iy8A Crystal structure of levodione reductase (see paper)
43% identity, 98% coverage: 2:247/250 of query aligns to 1:256/258 of 1iy8A
- active site: G15 (= G16), S143 (= S142), Q153 (≠ T152), Y156 (= Y155), K160 (= K159)
- binding nicotinamide-adenine-dinucleotide: G11 (= G12), S14 (= S15), G15 (= G16), L16 (≠ I17), D35 (= D36), V36 (≠ M37), A62 (≠ V62), D63 (= D63), V64 (= V64), N90 (= N90), G92 (= G92), I93 (= I93), T141 (≠ C140), S143 (= S142), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (≠ A186), T191 (= T190), P192 (= P191), M193 (≠ L192)
4urfB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
44% identity, 99% coverage: 1:247/250 of query aligns to 1:247/248 of 4urfB
- active site: G16 (= G16), S142 (= S142), I152 (≠ T152), Y155 (= Y155), K159 (= K159)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: L210 (≠ V210), R211 (≠ G211), R212 (= R212)
- binding bicarbonate ion: I92 (= I93), G94 (= G94), R109 (= R109), R179 (= R179), S228 (= S228)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), G14 (≠ A14), N15 (≠ S15), G16 (= G16), I17 (= I17), D36 (= D36), I37 (≠ M37), D62 (= D63), T63 (≠ V64), N89 (= N90), A90 (= A91), G91 (= G92), I140 (≠ C140), Y155 (= Y155), K159 (= K159), P185 (= P185), A186 (= A186), I188 (= I188), T190 (= T190)
4urfA Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
44% identity, 99% coverage: 1:247/250 of query aligns to 1:247/248 of 4urfA
- active site: G16 (= G16), S142 (= S142), I152 (≠ T152), Y155 (= Y155), K159 (= K159)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: I92 (= I93), S93 (vs. gap), G94 (= G94), E95 (≠ H95), T97 (≠ A97), E101 (≠ S101), T103 (≠ P103), Q106 (≠ D106), R109 (= R109), S175 (≠ A175), G177 (= G177)
- binding magnesium ion: S237 (≠ A237), Y238 (≠ N238)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), G14 (≠ A14), N15 (≠ S15), G16 (= G16), I17 (= I17), D36 (= D36), I37 (≠ M37), W41 (≠ R41), D62 (= D63), T63 (≠ V64), N89 (= N90), A90 (= A91), G91 (= G92), I140 (≠ C140), Y155 (= Y155), K159 (= K159), P185 (= P185), I188 (= I188), T190 (= T190)
4ureB Molecular genetic and crystal structural analysis of 1-(4- hydroxyphenyl)-ethanol dehydrogenase from aromatoleum aromaticum ebn1 (see paper)
44% identity, 99% coverage: 1:247/250 of query aligns to 1:247/248 of 4ureB
- active site: G16 (= G16), S142 (= S142), I152 (≠ T152), Y155 (= Y155), K159 (= K159)
- binding 3-pyridinium-1-ylpropane-1-sulfonate: N15 (≠ S15), G16 (= G16), I17 (= I17), N89 (= N90), G91 (= G92), Y155 (= Y155), P185 (= P185), A186 (= A186)
6zzsD Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and 3-oxovalerate (see paper)
41% identity, 98% coverage: 3:247/250 of query aligns to 5:260/261 of 6zzsD
- active site: G18 (= G16), S143 (= S142), Y156 (= Y155)
- binding nicotinamide-adenine-dinucleotide: G14 (= G12), S17 (= S15), I19 (= I17), D38 (= D36), M39 (= M37), D64 (= D63), V65 (= V64), N91 (= N90), A92 (= A91), G93 (= G92), M141 (≠ C140), A142 (= A141), S143 (= S142), Y156 (= Y155), K160 (= K159), P186 (= P185), G187 (≠ A186), V189 (≠ I188), T191 (= T190), L193 (= L192)
- binding 3-oxidanylidenepentanoic acid: Q95 (≠ G94), S143 (= S142), N145 (≠ L144), K153 (≠ T152), Y156 (= Y155), Q197 (vs. gap)
6zzqA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from acinetobacter baumannii complexed with NAD+ and acetoacetate (see paper)
41% identity, 98% coverage: 3:247/250 of query aligns to 4:259/260 of 6zzqA
- active site: G17 (= G16), S142 (= S142), Y155 (= Y155)
- binding acetoacetic acid: Q94 (≠ G94), S142 (= S142), K152 (≠ T152), Y155 (= Y155), Q196 (vs. gap)
- binding nicotinamide-adenine-dinucleotide: G13 (= G12), S16 (= S15), G17 (= G16), I18 (= I17), D37 (= D36), M38 (= M37), D63 (= D63), V64 (= V64), N90 (= N90), A91 (= A91), G92 (= G92), M140 (≠ C140), A141 (= A141), S142 (= S142), Y155 (= Y155), K159 (= K159), Y187 (= Y187), V188 (≠ I188), T190 (= T190)
4nbuB Crystal structure of fabg from bacillus sp (see paper)
39% identity, 98% coverage: 2:245/250 of query aligns to 4:242/244 of 4nbuB
- active site: G18 (= G16), N111 (= N114), S139 (= S142), Q149 (≠ T152), Y152 (= Y155), K156 (= K159)
- binding acetoacetyl-coenzyme a: D93 (≠ M96), K98 (≠ S101), S139 (= S142), N146 (≠ Q149), V147 (≠ S150), Q149 (≠ T152), Y152 (= Y155), F184 (≠ Y187), M189 (≠ L192), K200 (≠ R203)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G12), N17 (≠ S15), G18 (= G16), I19 (= I17), D38 (= D36), F39 (≠ M37), V59 (= V62), D60 (= D63), V61 (= V64), N87 (= N90), A88 (= A91), G89 (= G92), I90 (= I93), T137 (≠ C140), S139 (= S142), Y152 (= Y155), K156 (= K159), P182 (= P185), F184 (≠ Y187), T185 (≠ I188), T187 (= T190), M189 (≠ L192)
5h5xC Crystal structure of nadh bound carbonyl reductase from streptomyces coelicolor
49% identity, 97% coverage: 6:247/250 of query aligns to 14:256/257 of 5h5xC
- active site: G24 (= G16), S151 (= S142), Y164 (= Y155), K168 (= K159)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G20 (= G12), S23 (= S15), G24 (= G16), I25 (= I17), D44 (= D36), F45 (≠ M37), L69 (≠ V62), D70 (= D63), N97 (= N90), A98 (= A91), Y164 (= Y155), K168 (= K159), P194 (= P185), G195 (≠ A186), I197 (= I188), T199 (= T190)
7tzpG Crystal structure of putataive short-chain dehydrogenase/reductase (fabg) from klebsiella pneumoniae subsp. Pneumoniae ntuh-k2044 in complex with nadh (see paper)
42% identity, 98% coverage: 1:245/250 of query aligns to 4:245/247 of 7tzpG
- binding 1,4-dihydronicotinamide adenine dinucleotide: G15 (= G12), R18 (≠ S15), G19 (= G16), I20 (= I17), D39 (= D36), R40 (≠ M37), C63 (≠ V62), I65 (≠ V64), N91 (= N90), G93 (= G92), I94 (= I93), V114 (≠ I113), Y155 (= Y155), K159 (= K159), I188 (= I188), T190 (= T190), T193 (≠ L193)
4nbtA Crystal structure of fabg from acholeplasma laidlawii (see paper)
38% identity, 98% coverage: 2:245/250 of query aligns to 2:237/239 of 4nbtA
- active site: G16 (= G16), S132 (= S142), Y145 (= Y155), K149 (= K159)
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), K15 (≠ S15), G16 (= G16), L17 (≠ I17), D36 (= D36), L37 (= L49), L52 (≠ V62), N53 (≠ D63), V54 (= V64), N80 (= N90), A81 (= A91), G82 (= G92), I130 (≠ C140), S132 (= S142), Y145 (= Y155), K149 (= K159), P177 (= P185), G178 (≠ A186), I180 (= I188), T182 (= T190)
7v1qA Leifsonia alcohol dehydrogenases lnadh (see paper)
45% identity, 98% coverage: 3:247/250 of query aligns to 6:250/251 of 7v1qA
- binding nicotinamide-adenine-dinucleotide: G15 (= G12), S18 (= S15), G19 (= G16), I20 (= I17), D39 (= D36), L40 (≠ M37), D65 (= D63), V66 (= V64), N91 (= N90), M142 (≠ C140), Y157 (= Y155), K161 (= K159), P187 (= P185), G188 (≠ A186), F189 (≠ Y187), I190 (= I188)
H9XP47 Meso-2,3-butanediol dehydrogenase; BDH; meso-2,3-BDH; (R,S)-butane-2,3-diol dehydrogenase; NAD(H)-dependent meso-2,3-BDH; SmBdh; EC 1.1.1.- from Serratia marcescens (see paper)
41% identity, 99% coverage: 1:247/250 of query aligns to 1:243/251 of H9XP47
- N15 (≠ S15) binding NAD(+)
- M17 (≠ I17) binding NAD(+)
- D36 (= D36) binding NAD(+)
- D60 (= D63) binding NAD(+)
- V61 (= V64) binding NAD(+)
- N87 (= N90) binding NAD(+)
- S138 (= S142) binding (R)-acetoin; binding (S)-acetoin
- V139 (≠ I143) mutation to Q: Retains 50% of activity with acetoin as substrate; when associated with A-247.
- S140 (≠ L144) binding (R)-acetoin
- Y151 (= Y155) binding (R)-acetoin; binding (S)-acetoin; binding NAD(+)
- K155 (= K159) binding NAD(+)
- V184 (≠ I188) binding NAD(+)
- T186 (= T190) binding NAD(+)
- RDK 197:199 (≠ LDR 201:203) mutation to SEAAGKPLGYGTET: Mimics longer alpha6 helix. Retains 3% of activity with acetoin as substrate.
Sites not aligning to the query:
- 247 Q→A: Retains 10% of activity with acetoin as substrate. Retains 50% of activity with acetoin as substrate; when associated with Q-139.
Q6WVP7 NADP-dependent (R)-specific alcohol dehydrogenase; (R)-specific ADH; Ketoreductase; KRED; EC 1.1.1.- from Lentilactobacillus kefiri (Lactobacillus kefiri) (see paper)
41% identity, 98% coverage: 2:247/250 of query aligns to 4:251/252 of Q6WVP7
Sites not aligning to the query:
7djsD Crystal structure of isopiperitenol dehydrogenase from pseudomonas aeruginosa complexed with NAD
42% identity, 98% coverage: 3:247/250 of query aligns to 3:250/251 of 7djsD
- binding nicotinamide-adenine-dinucleotide: G12 (= G12), G16 (= G16), I17 (= I17), D36 (= D36), L37 (≠ M37), C61 (≠ V62), D62 (= D63), V63 (= V64), N89 (= N90), A90 (= A91), T140 (≠ C140), S142 (= S142), Y155 (= Y155), K159 (= K159), A186 (= A186), V187 (≠ Y187)
Query Sequence
>WP_018231642.1 NCBI__GCF_000378965.1:WP_018231642.1
MRLKDKVAIITGGASGIGEASALDMVAEGARVVIADMDSDRGEALARRLNDMHEASAVFQ
AVDVADEGQVEALFQATLSHFGRVDVVFNNAGIGHMAEADSYPTEDWQRVIDINLTGVFL
VAKHALGIMKAQGSGSIVNCASILGNVGQSMTSAYSAAKGGVVNLTRTLALEYAAHGIRI
NTVSPAYIDTPLLRDLDREMLDRLIALHPVGRLGRPEEVARAVTFLASDDASFITGANLL
VDGGFTAGKS
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory