SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_018370772.1 NCBI__GCF_000341525.1:WP_018370772.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

P69797 PTS system mannose-specific EIIAB component; EIIAB-Man; EIII-Man; EC 2.7.1.191 from Escherichia coli (strain K12) (see 4 papers)
40% identity, 99% coverage: 1:325/329 of query aligns to 1:320/323 of P69797

query
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P69797

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M1 (= M1) modified: Initiator methionine, Removed
H10 (= H10) mutation to C: Loss of phosphotransferase activity. Unable to dimerize.; mutation to E: Results in the formation of a single complex corresponding to the productive phosphoryl transfer complex.
W12 (≠ E12) mutation to F: Slight phosphotransferase activity. Unable to dimerize.
K48 (= K48) mutation to C: Retains more than 50% of phosphotransferase activity.
K55 (≠ A55) modified: N6-acetyllysine
S72 (= S72) mutation to C: Slight phosphotransferase activity. Unable to dimerize.
H86 (≠ K88) mutation to N: Loss of phosphotransferase activity.
S110 (≠ G114) mutation to C: Retains more than 50% of phosphotransferase activity.
H175 (= H180) mutation to C: Loss of phosphotransferase activity.
H219 (≠ N224) mutation to Q: Slight phosphotransferase activity.
K234 (≠ R239) modified: N6-acetyllysine

P26380 PTS system fructose-specific EIIB component; EIIB-Fru; Fructose-specific phosphotransferase enzyme IIB component; lev-PTS; p18; EC 2.7.1.202 from Bacillus subtilis (strain 168) (see paper)
43% identity, 48% coverage: 167:324/329 of query aligns to 2:159/163 of P26380

query
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P26380

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H15 (= H180) active site, Pros-phosphohistidine intermediate; modified: Phosphohistidine; by EIIA; mutation to A: Unable to be phosphorylated.

1vrcB Complex of enzyme iiamannose and the histidine-containing phosphocarrier protein hpr from escherichia coli nmr, restrained regularized mean structure (see paper)
35% identity, 40% coverage: 2:133/329 of query aligns to 1:128/129 of 1vrcB

query
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1vrcB

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binding phosphite ion: H9 (= H10), F35 (= F36), G70 (= G71), S71 (= S72)

P26379 PTS system fructose-specific EIIA component; EIIA-Fru; Fructose-specific phosphotransferase enzyme IIA component; lev-PTS; p16 from Bacillus subtilis (strain 168) (see paper)
33% identity, 32% coverage: 3:107/329 of query aligns to 2:104/146 of P26379

query
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P26379

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H9 (= H10) active site, Tele-phosphohistidine intermediate; modified: Phosphohistidine; by HPr; mutation to A: Phosphorylation is only slightly increased.

Query Sequence

>WP_018370772.1 NCBI__GCF_000341525.1:WP_018370772.1
MSIGIIIASHGEFASGIHQSGSMIFGDQEKVQVVTFMPSEGPDDLYAKFNNAVAAFDADD
EVLVLADLWSGSPFNQASRVMGENPDRKFAIITGLNLPMLIQAYTERMMDANAGVEAVAA
NIIKEAKDGVKALPEELNPAEVTATNAAAPVAQTAIPEGTVIGDGKLKINLARIDTRLLH
GQVATAWTPDSKADRIIVASDSVSQDSLRKELIKQAAPGNVKANVVPIDKLIAAAKDPRF
GGTHALILFETPQDALRAIEGGVPIKTLNVGSMAHSTGKTMVNNVLSMDKDDVATFEKLR
DLGVTFDVRKVPNDSKKDLFDLINKANVQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory