SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_018869678.1 NCBI__GCF_001995255.1:WP_018869678.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4bjhB Crystal structure of the aquifex reactor complex formed by dihydroorotase (h180a, h232a) with dihydroorotate and aspartate transcarbamoylase with n-(phosphonacetyl)-l-aspartate (pala) (see paper)
40% identity, 90% coverage: 30:331/336 of query aligns to 1:290/291 of 4bjhB

query
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4bjhB

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active site: R47 (= R83), T48 (= T84), K75 (= K111), R97 (≠ H134), H126 (= H163), Q129 (= Q166)
binding n-(phosphonacetyl)-l-aspartic acid: S45 (= S81), T46 (= T82), R47 (= R83), T48 (= T84), R97 (≠ H134), H126 (= H163), R159 (= R196), V160 (= V197), R213 (= R251), Q215 (= Q253), G251 (= G292)

3d6nB Crystal structure of aquifex dihydroorotase activated by aspartate transcarbamoylase (see paper)
40% identity, 90% coverage: 30:331/336 of query aligns to 1:290/291 of 3d6nB

query
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3d6nB

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active site: R47 (= R83), T48 (= T84), K75 (= K111), R97 (≠ H134), H126 (= H163), Q129 (= Q166)
binding citrate anion: T48 (= T84), R97 (≠ H134), H126 (= H163), R159 (= R196), V160 (= V197), R213 (= R251), G251 (= G292)

3r7fA Crystal structure of cp-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
39% identity, 89% coverage: 30:327/336 of query aligns to 1:285/291 of 3r7fA

query
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3r7fA

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active site: R49 (= R83), T50 (= T84), K77 (= K111), R99 (= R133), H127 (= H163), Q130 (= Q166), L210 (= L250), P249 (= P291), G277 (= G319)
binding phosphoric acid mono(formamide)ester: S47 (= S81), T48 (= T82), R49 (= R83), T50 (= T84), R99 (= R133), H127 (= H163), Q130 (= Q166), P249 (= P291), A250 (≠ G292)
binding phosphate ion: S11 (≠ N40), T12 (≠ R41), Q23 (≠ E52), K26 (= K60), E140 (≠ R176), R171 (≠ I207), K241 (≠ S283), H243 (≠ T285), K272 (≠ E314), K272 (≠ E314), K275 (≠ T317)

3r7dA Crystal structure of unliganded aspartate transcarbamoylase from bacillus subtilis (see paper)
39% identity, 89% coverage: 30:327/336 of query aligns to 1:285/291 of 3r7dA

query
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3r7dA

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active site: R49 (= R83), T50 (= T84), K77 (= K111), R99 (= R133), H127 (= H163), Q130 (= Q166), L210 (= L250), P249 (= P291), G277 (= G319)
binding phosphate ion: S11 (≠ N40), T12 (≠ R41), T73 (≠ S107), S74 (= S108), K77 (= K111), R171 (≠ I207)

3r7lA Crystal structure of pala-bound aspartate transcarbamoylase from bacillus subtilis (see paper)
39% identity, 89% coverage: 30:327/336 of query aligns to 1:285/290 of 3r7lA

query
sites
3r7lA

L

M

R

K

H

H

M

L

L

T

T

T

I

M

E

S

G

E

L

L

N

S

R

T

T

E

L

E

L

I

T

K

E

D

I

L

L

L

D

Q

T

T

A

A

E

Q

S

E

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

S

V

G

K

K

K

T

V

D

P

N

L

Q

L

L

R

T

G

G

K

K

T

F

I

A

V

A

N

N

L

L

F

F

E

E

A

P

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

F

T

S

F

F

E

E

L

V

A

A

A

E

K

K

R

K

L

L

S

G

A

M

D

N

V

V

L

L

N

N

I

L

N

D

V

G

S

T

T

S

S

T

S
|
S

A

V

V

Q

K
|
K

G

G

E

E

S

T

L

L

L

Y

D

D

T

T

L

I

R

R

N

T

L

L

E

E

A

S

M

I

N

G

V

V

D

D

M

V

F

C

V

V

V

I

R
|
R

H

H

E

S

E

E

S

D

G

-

T

-

A

E

H

Y

F

Y

I

E

A

E

R

L

H

V

A

S

A

Q

P

V

G

N

I

I

S

P

V

I

L

L

N

N

A

A

G

G

D

D

G

G

R

C

H

G

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

S

L

L

D

D

M

L

F

M

T

T

I

I

R

Y

R

E

H

E

K

F

G

N

D

T

F

F

T

K

K

G

L

L

K

T

V

V

A

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R
|
R

V

V

A

A

R

R

S

S

Q

N

I

A

H

E

A

V

L

L

N

T

I

R

L

L

Q

-

T

-

G

G

E

A

V

R

R

V

V

L

V

F

A

S

P

G

R

P

S

S

L

E

L

W

P

Q

A

D

D

E

V

E

E

N

Q

T

L

F

G

G

V

T

H

Y

V

V

H

-

D

S

L

M

D

D

S

E

G

A

L

V

Q

E

D

S

V

S

D

D

V

V

I

M

M

L

L
|
L

R
|
R

L

I

Q
|
Q

R

N

E

E

R

R

M

-

E

-

H

H

A

Q

H

S

L

A

P

V

S

S

E

Q

H

E

E

G

Y

Y

F

L

Q

N

R

K

F

Y

G

G

L

L

T

T

A

V

R

E

R

R

L

A

Q

E

L

R

A

M

S

K

P

R

T

H

C

A

L

I

V

I

M

M

H

H

P
|
P

G
x
A

P

P

A

V

N

N

R

R

G

G

V

V

E

E

I

I

E

D

S

D

A

S

V

L

A

V

D

E

G

S

P

E

H

K

S

S

V

R

I

I

L

F

E

K

Q

Q

V

M

T

K

Y

N

G
|
G

I

V

S

F

V

I

R

R

M

M

A

A

V

V

M

I

active site: R49 (= R83), T50 (= T84), K77 (= K111), R99 (= R133), H127 (= H163), Q130 (= Q166), L210 (= L250), P249 (= P291), G277 (= G319)
binding n-(phosphonacetyl)-l-aspartic acid: S47 (= S81), T48 (= T82), R49 (= R83), T50 (= T84), S74 (= S108), K77 (= K111), R99 (= R133), H127 (= H163), R160 (= R196), R211 (= R251), Q213 (= Q253), A250 (≠ G292)

P05654 Aspartate carbamoyltransferase catalytic subunit; Aspartate transcarbamylase; ATCase; EC 2.1.3.2 from Bacillus subtilis (strain 168) (see paper)
39% identity, 89% coverage: 30:327/336 of query aligns to 1:285/304 of P05654

query
sites
P05654

L

M

R

K

H

H

M

L

L

T

T

T

I

M

E

S

G

E

L

L

N

S

R

T

T

E

L

E

L

I

T

K

E

D

I

L

L

L

D

Q

T

T

A

A

E

Q

S

E

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

S

V

G

K

K

K

T

V

D

P

N

L

Q

L

L

R

T

G

G

K

K

T

F

I

A

V

A

N

N

L

L

F

F

E

E

A

P

S

S

T

T

R

R

T

T

R

R

T

F

T

S

F

F

E

E

L

V

A

A

A

E

K

K

R

K

L

L

S

G

A

M

D

N

V

V

L

L

N

N

I

L

N

D

V

G

S

T

T

S

S

T

S

S

A

V

V

Q

K

K

G

G

E

E

S

T

L

L

L

Y

D

D

T

T

L

I

R

R

N

T

L

L

E

E

A

S

M

I

N

G

V

V

D

D

M

V

F

C

V

V

V

I

R

R

H

H

E

S

E

E

S

D

G

-

T

-

A

E

H

Y

F

Y

I

E

A

E

R

L

H

V

A

S

A

Q

P

V

G

N

I

I

S

P

V

I

L

L

N

N

A

A

G

G

D

D

G

G

R

C

H

G

A

Q

H

H

P

P

T

T

Q

Q

A

S

L

L

D

D

M

L

F

M

T

T

I

I

R

Y

R

E

H

E

K

F

G

N

D

T

F

F

T

K

K

G

L

L

K

T

V

V

A

S

I

I

V

H

G

G

D

D

I

I

L

K

H

H

S

S

R

R

V

V

A

A

R

R

S

S

Q

N

I

A

H

E

A

V

L

L

N

T

I

R

L

L

Q

-

T

-

G

G

E

A

V

R

R

V

V

L

V

F

A

S

P

G

R

P

S

S

L

E

L

W

P

Q

A

D

D

E

V

E

E

N

Q

T

L

F

G

G

V

T

H

Y

V

V

H

-

D

S

L

M

D

D

S

E

G

A

L

V

Q

E

D

S

V

S

D

D

V

V

I

M

M

L

L

L

R

R

L

I

Q

Q

R

N

E

E

R

R

M

-

E

-

H

H

A

Q

H

S

L

A

P

V

S

S

E

Q

H

E

E

G

Y

Y

F

L

Q

N

R

K

F

Y

G

G

L

L

T

T

A

V

R

E

R

R

L

A

Q

E

L

R

A

M

S

K

P

R

T

H

C

A

L

I

V

I

M

M

H

H

P

P

G

A

P

P

A

V

N

N

R

R

G

G

V

V

E

E

I

I

E

D

S

D

A

S

V

L

A

V

D

E

G

S

P

E

H

K

S

S

V

R

I

I

L

F

E

K

Q

Q

V

M

T

K

Y

N

G

G

I

V

S

F

V

I

R

R

M

M

A

A

V

V

M

I

Sites not aligning to the query:

303 modified: Phosphoserine

6pnzA The structure of the aspartate transcarbamoylase trimer from staphylococcus aureus complexed with pala at 2.27 resolution.
36% identity, 90% coverage: 30:331/336 of query aligns to 1:292/293 of 6pnzA

query
sites
6pnzA

L

M

R

N

H

H

M

L

L

L

T

S

I

M

E

E

G

H

L

L

N

S

R

T

T

D

L

Q

L

I

T

Y

E

K

I

L

L

I

D

Q

T

K

A

A

E

S

S

Q

F

F

A

-

N

-

M

-

N

-

E

K

K

S

A

G

V

E

K

R

K

Q

V

L

P

P

L

N

L

F

R

E

G

G

K

K

T

Y

I

V

V

A

N

N

L

L

F

F

E

E

A

N

S
|
S

T
|
T

R
|
R

T
|
T

R

K

T

C

T

S

F

F

E

E

L

M

A

A

A

E

K

L

R

K

L

L

S

G

A

L

D

K

V

T

L

I

N

S

I

F

N

E

V

T

S

S

T

T

S

S

S
|
S

A

V

V

S

K
|
K

G

G

E

E

S

S

L

L

L

Y

D

D

T

T

L

C

R

K

N

T

L

L

E

E

A

S

M

I

N

G

V

C

D

D

M

L

F

L

V

V

V

I

R
|
R

H

H

E

P

E

F

S

N

G

-

T

-

A

-

H

N

F

Y

I

Y

A

E

R

K

H

L

A

A

A

N

P

I

G

N

I

I

S

P

V

I

L

A

N

N

A

A

G

G

D

D

G

G

R

S

H

G

A

Q

H
|
H

P

P

T

T

Q

Q

A

S

L

L

D

D

M

L

F

M

T

T

I

I

R

Y

R

E

H

E

K

Y

G

G

D

Y

F

F

T

E

K

G

L

L

K

N

V

V

A

L

I

I

V

C

G

G

D

D

I

I

L

K

H

N

S

S

R
|
R

V

V

A

A

R

R

S

S

Q

N

I

Y

H

H

A

S

L

L

N

K

I

A

L

L

Q

-

T

G

G

A

E

N

V

V

R

M

V

F

V

N

A

S

P

P

R

N

S

A

L

W

L

I

P

D

A

D

D

S

V

L

E

E

Q

A

L

P

G

Y

V

V

H

N

V

I

H

-

D

-

L

-

D

D

S

D

G

V

L

I

Q

E

D

T

V

V

D

D

V

I

V

V

I

M

M

L

L

L

R
|
R

L

I

Q
|
Q

R

H

E

E

R

R

-

H

-

G

-

L

M

A

E

E

H

E

A

T

H

R

L

F

P

A

S

A

E

D

H

-

E

D

Y

Y

F

H

Q

Q

R

K

F

H

G

G

L

L

T

N

A

E

R

V

R

R

L

Y

Q

N

L

K

A

L

S

Q

P

E

T

H

C

A

L

I

V

V

M

M

H

H

P

P

G
x
A

P

P

A

V

N

N

R

R

G

G

V

V

E

E

I

I

E

Q

S

S

A

D

V

L

A

V

D

E

G

A

P

S

H

K

S

S

V

R

I

I

L

F

E

K

Q

Q

V

M

T

E

Y

N

G

G

I

V

S

Y

V

L

R

R

M

M

A

A

V

V

M

I

S

D

M

E

V

L

M

L

binding n-(phosphonacetyl)-l-aspartic acid: S48 (= S81), T49 (= T82), R50 (= R83), T51 (= T84), S75 (= S108), K78 (= K111), R100 (= R133), H127 (= H163), R160 (= R196), R210 (= R251), Q212 (= Q253), A253 (≠ G292)

4eknB Structure of the catalytic chain of methanococcus jannaschii aspartate transcarbamoylase in a hexagonal crystal form (see paper)
34% identity, 90% coverage: 30:331/336 of query aligns to 1:300/304 of 4eknB

query
sites
4eknB

L

M

R

K

H

H

M

L

L

I

T

S

I

M

E

K

G

D

L

I

N

G

R

K

T

E

L

E

L

I

T

L

E

E

I

I

L

L

D

D

T

E

A

A

E

R

S

K

F

M

A

E

N

E

M

L

-

L

N

N

E

T

K

K

A

-

V

-

K

R

K

P

V

L

P

K

L

L

R

E

G

G

K

K

T

I

I

L

V

A

N

T

L

V

F

F

F

Y

E

E

A

P

S

S

T

T

R

R

T

T

R

R

T

L

T

S

F

F

E

E

L

T

A

A

A

M

K

K

R

R

L

L

S

G

A

G

D

E

V

V

L

I

N

T

I

M

-

T

N

D

V

L

S

K

T

S

S

S

A

V

V

A

K

K

G

G

E

E

S

S

L

L

L

I

D

D

T

T

L

I

R

R

N

V

L

I

E

S

A

G

M

Y

N

-

V

A

D

D

M

I

F

I

V

V

V

L

R

R

H

H

E

P

E

S

S

E

G

G

T

A

A

A

H

R

F

L

I

A

A

S

R

E

H

Y

A

S

A

Q

P

-

G

-

I

V

S

P

V

I

L

I

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H

H

P

P

T

T

Q

Q

A

T

L

L

D

D

M

L

F

Y

T

T

I

I

R

M

R

R

H

E

K

I

G

G

D

R

F

I

T

D

K

G

L

I

K

K

V

I

A

A

I

F

V

V

G

G

D

D

I

L

L

K

H

Y

S
x
G

R
|
R

V
x
T

A

V

R

H

S

S

Q

L

I

V

H

Y

A

A

L

L

N

S

I

L

L

F

Q

E

T

N

G

V

E

E

V

M

R

Y

V

F

V

V

A

S

P

P

R

K

S

E

L

L

-

R

L

L

P

P

A

K

D

D

V

I

-

I

-

E

-

D

-

L

-

K

-

A

E

K

Q

N

L

I

G

K

V

F

H

Y

V

E

H

K

H

E

D

S

L

L

D

D

S

D

G

L

L

D

Q

D

D

D

V

I

D

D

V

V

V

L

I

Y

M

V

L
x
T

R
|
R

L

I

Q

Q

R

K

E

E

R

R

M

-

E

-

H

-

A

-

H

-

L

F

P

P

S

D

E

P

H

N

E

E

Y

Y

F

E

Q

K

R

V

F

K

G

G

-

S

L

Y

T

K

A

I

R

K

R

R

L

E

Q

Y

L

V

A

E

S

G

P

K

T

K

C

F

L

I

V

I

M

M

H

H

P
|
P

G

L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

D

S

Y

A

D

V

V

A

D

D

D

G

L

P

P

H

Q

S

A

V

K

I

Y

L

F

E

K

Q

Q

V

S

T

F

Y

Y

G
|
G

I

I

S

P

V

V

R

R

M

M

A

A

V

I

M

L

S

K

M

K

V

L

M

I

active site: T225 (≠ L250), P262 (= P291), G288 (= G319)
binding sulfate ion: G163 (≠ S195), R164 (= R196), T165 (≠ V197), R226 (= R251)

2ipoA E. Coli aspartate transcarbamoylase complexed with n-phosphonacetyl-l- asparagine (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 2ipoA

query
sites
2ipoA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding n~2~-(phosphonoacetyl)-l-asparagine: S52 (= S81), T53 (= T82), R54 (= R83), T55 (= T84), R105 (= R133), H134 (= H163), R167 (= R196), T168 (≠ V197), R229 (= R251), L267 (≠ G292)

2h3eA Structure of wild-type e. Coli aspartate transcarbamoylase in the presence of n-phosphonacetyl-l-isoasparagine at 2.3a resolution (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 2h3eA

query
sites
2h3eA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V

T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding (s)-4-amino-4-oxo-3-(2-phosphonoacetamido)butanoic acid: S52 (= S81), T53 (= T82), R54 (= R83), T55 (= T84), R105 (= R133), H134 (= H163), R167 (= R196), R229 (= R251), L267 (≠ G292)

2fzkA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.50 resolution (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 2fzkA

query
sites
2fzkA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S

S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R

R

V
x
T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R
|
R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding 3,5-bis[(phosphonoacetyl)amino]benzoic acid: T55 (= T84), H134 (= H163), Q137 (= Q166), T168 (≠ V197), R229 (= R251), P266 (= P291), L267 (≠ G292), R296 (= R323)

2fzgA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.25 resolution (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 2fzgA

query
sites
2fzgA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding {1,3-phenylenebis[imino(2-oxoethane-2,1-diyl)]}bis(phosphonic acid): S52 (= S81), R54 (= R83), T55 (= T84), R105 (= R133), H134 (= H163), R167 (= R196), T168 (≠ V197), R229 (= R251), P266 (= P291), L267 (≠ G292)

2fzcA The structure of wild-type e. Coli aspartate transcarbamoylase in complex with novel t state inhibitors at 2.10 resolution (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 2fzcA

query
sites
2fzcA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding {ethane-1,2-diylbis[imino(2-oxoethane-2,1-diyl)]}bis(phosphonic acid): S52 (= S81), R54 (= R83), T55 (= T84), R105 (= R133), R167 (= R196), T168 (≠ V197), P266 (= P291), L267 (≠ G292)

2airA T-state active site of aspartate transcarbamylase:crystal structure of the carbamyl phosphate and l-alanosine ligated enzyme (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 2airA

query
sites
2airA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R

R

V

T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G

L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding 3-[hydroxy(nitroso)amino]-l-alanine: S52 (= S81), T53 (= T82), R54 (= R83), R105 (= R133)
binding phosphoric acid mono(formamide)ester: R54 (= R83), T55 (= T84), R105 (= R133), H134 (= H163)

1za2A Structure of wild-type e. Coli aspartate transcarbamoylase in the presence of ctp, carbamoyl phosphate at 2.50 a resolution (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 1za2A

query
sites
1za2A

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S

S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V
x
T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G
x
L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding phosphoric acid mono(formamide)ester: T53 (= T82), R54 (= R83), T55 (= T84), R105 (= R133), R167 (= R196), T168 (≠ V197), L267 (≠ G292)

1r0cA Products in the t state of aspartate transcarbamylase: crystal structure of the phosphate and n-carbamyl-l-aspartate ligated enzyme (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 1r0cA

query
sites
1r0cA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R

R

V

T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G

L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding n-carbamoyl-l-aspartate: S52 (= S81), R54 (= R83), R105 (= R133)
binding phosphate ion: R105 (= R133), H134 (= H163), Q137 (= Q166)

1r0bA Aspartate transcarbamylase (atcase) of escherichia coli: a new crystalline r state bound to pala, or to product analogues phosphate and citrate (see paper)
36% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 1r0bA

query
sites
1r0bA

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S

S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S
|
S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V

T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q
|
Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G

L

P
|
P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding citrate anion: H134 (= H163), R167 (= R196), R229 (= R251), Q231 (= Q253), P266 (= P291), P268 (= P293)
binding phosphate ion: S80 (= S107), K84 (= K111)

P0A786 Aspartate carbamoyltransferase catalytic subunit; Aspartate transcarbamylase; ATCase; EC 2.1.3.2 from Escherichia coli (strain K12) (see 4 papers)
36% identity, 90% coverage: 31:332/336 of query aligns to 8:306/311 of P0A786

query
sites
P0A786

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S

S

T

T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

E

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K

K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

R

E

H

T

K

Q

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R

R

V

T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

Q

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L

T

R

R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P

P

G
x
L

P
|
P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G

G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

R55 (= R83) binding carbamoyl phosphate
T56 (= T84) binding carbamoyl phosphate
R106 (= R133) binding carbamoyl phosphate
H135 (= H163) binding carbamoyl phosphate
Q138 (= Q166) binding carbamoyl phosphate
L268 (≠ G292) binding carbamoyl phosphate
P269 (= P293) binding carbamoyl phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2at1A Crystal structures of phosphonoacetamide ligated t and phosphonoacetamide and malonate ligated r states of aspartate carbamoyltransferase at 2.8-angstroms resolution and neutral ph (see paper)
35% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 2at1A

query
sites
2at1A

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

Q

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

H

T

K

E

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V

T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

E

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
|
R

L

V

Q

Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
|
P

G

L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
|
G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding alpha-D-glucopyranose: R167 (= R196), R229 (= R251)
binding phosphonoacetamide: S52 (= S81), T53 (= T82), R54 (= R83), T55 (= T84), R105 (= R133), H134 (= H163), Q137 (= Q166)

1at1A Crystal structures of phosphonoacetamide ligated t and phosphonoacetamide and malonate ligated r states of aspartate carbamoyltransferase at 2.8-angstroms resolution and neutral p H (see paper)
35% identity, 90% coverage: 31:332/336 of query aligns to 7:305/310 of 1at1A

query
sites
1at1A

R

K

H

H

M

I

L

I

T

S

I

I

E

N

G

D

L

L

N

S

R

R

T

D

L

D

L

L

T

N

E

L

I

V

L

L

D

A

T

T

A

A

E

A

S

K

F

L

A

-

N

-

M

-

N

-

E

-

K

K

A

A

V

N

K

P

K

Q

V

P

P

E

L

L

R

K

G

H

K

K

T

V

I

I

V

A

N

S

L

C

F

F

E

E

A

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

T

L

T

S

F

F

E

Q

L

T

A

S

A

M

K

H

R

R

L

L

S

G

A

A

D

S

V

V

L

V

N

G

I

F

N

S

V

D

S

S

-

A

-

N

T

T

S

S

S

L

A

G

V

K

K
|
K

G

G

E

E

S

T

L

L

L

A

D

D

T

T

L

I

R

S

N

V

L

I

E

S

A

T

M

Y

N

-

V

V

D

D

M

A

F

I

V

V

V

M

R
|
R

H

H

E

P

E

Q

S

E

G

G

T

A

A

A

H

R

F

-

I

L

A

A

R

T

H

E

A

F

A

S

P

G

G

N

I

V

S

P

V

V

L

L

N

N

A

A

G

G

D

D

G

G

R

S

H

N

A

Q

H
|
H

P

P

T

T

Q
|
Q

A

T

L

L

D

D

M

L

F

F

T

T

I

I

R

Q

H

T

K

E

G

G

D

R

F

L

T

D

K

N

L

L

K

H

V

V

A

A

I

M

V

V

G

G

D

D

I

L

L

K

H

Y

S

G

R
|
R

V

T

A

V

R

H

S

S

Q

L

I

T

H

Q

A

A

L

L

N

A

I

K

L

F

Q

D

T

G

G

N

E

R

V

F

R

Y

V

F

V

I

A

A

P

P

R

D

S

A

L

L

-

A

L

M

P

P

A

E

-

Y

-

I

-

L

-

D

-

M

-

L

D

D

V

E

E

K

Q

G

L

I

G

A

V

W

H

S

V

L

H

H

H

S

D

S

L

I

D

E

S

E

G

V

L

M

Q

A

D

E

V

V

D

D

V

I

V

L

I

Y

M

M

L
x
T

R
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R

L

V

Q
|
Q

R

K

E

E

R

R

M

L

E

D

H

-

A

-

H

-

L

-

P

P

S

S

E

E

H

Y

E

A

Y

N

F

V

Q

K

-

A

R

Q

F

F

G

V

L

L

T

R

A

A

R

S

R

D

L

L

Q

H

L

N

A

A

S

K

P

A

T

N

C

M

L

K

V

V

M

L

H

H

P
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P

G

L

P

P

A

-

N

-

R

R

G

V

V

D

E

E

I

I

E

A

S

T

A

D

V

V

A

D

D

K

G

T

P

P

H

H

S

A

V

W

I

Y

L

F

E

Q

Q

Q

V

A

T

G

Y

N

G
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G

I

I

S

F

V

A

R

R

M

Q

A

A

V

L

M

L

S

A

M

L

V

V

M

L

G

N

active site: R54 (= R83), T55 (= T84), K84 (= K111), R105 (= R133), H134 (= H163), Q137 (= Q166), T228 (≠ L250), P266 (= P291), G292 (= G319)
binding malonate ion: H134 (= H163), R167 (= R196), R229 (= R251), Q231 (= Q253)
binding phosphonoacetamide: S52 (= S81), T53 (= T82), R54 (= R83), T55 (= T84), R105 (= R133), H134 (= H163), Q137 (= Q166)

Query Sequence

>WP_018869678.1 NCBI__GCF_001995255.1:WP_018869678.1
MTELPAAHTSRLPQPGPLPNRLQLTDEGQLRHMLTIEGLNRTLLTEILDTAESFANMNEK
AVKKVPLLRGKTIVNLFFEASTRTRTTFELAAKRLSADVLNINVSTSSAVKGESLLDTLR
NLEAMNVDMFVVRHEESGTAHFIARHAAPGISVLNAGDGRHAHPTQALLDMFTIRRHKGD
FTKLKVAIVGDILHSRVARSQIHALNILQTGEVRVVAPRSLLPADVEQLGVHVHHDLDSG
LQDVDVVIMLRLQRERMEHAHLPSEHEYFQRFGLTARRLQLASPTCLVMHPGPANRGVEI
ESAVADGPHSVILEQVTYGISVRMAVMSMVMGAHQS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory