SitesBLAST

Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
30% identity, 92% coverage: 1:320/348 of query aligns to 1:288/309 of Q53W83

query
sites
Q53W83

M

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E

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V

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Y

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G

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G
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G

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x
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E
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x
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A

A

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A

L

L

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A

N

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F

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N

D

D

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x
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x
D

H

H

GAEVN 34:38 (≠ GGESN 34:38) binding substrate
YYR 103:105 (≠ YDR 104:106) binding substrate
R167 (= R169) binding substrate
S193 (≠ N198) binding ATP
219:225 (vs. 236:258, 0% identical) binding ATP
GAGD 248:251 (≠ GGGD 285:288) binding ATP
D251 (= D288) binding substrate
N275 (= N307) binding ATP
D287 (≠ K319) binding substrate

1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
30% identity, 92% coverage: 1:320/348 of query aligns to 1:288/301 of 1v1aA

query
sites
1v1aA

M

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x
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S

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M

A

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I

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Y

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x
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x
D

H

H

active site: G248 (= G285), A249 (≠ G286), G250 (= G287), D251 (= D288)
binding adenosine-5'-diphosphate: K219 (≠ E236), G221 (≠ A254), A222 (≠ S255), A249 (≠ G286), G250 (= G287), N275 (= N307), A279 (≠ F311)
binding 2-keto-3-deoxygluconate: L11 (≠ M11), G34 (= G34), A35 (≠ G35), N38 (= N38), Y89 (= Y89), R105 (= R106), R167 (= R169), G248 (= G285), D251 (= D288), D287 (≠ K319)

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
30% identity, 92% coverage: 1:320/348 of query aligns to 1:288/300 of 1v1bA

query
sites
1v1bA

M

M

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G

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x
G

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M

A

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A

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x
A

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A

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H

H

active site: G248 (= G285), A249 (≠ G286), G250 (= G287), D251 (= D288)
binding adenosine-5'-triphosphate: K219 (≠ E236), G221 (≠ A254), A238 (≠ Q274), F239 (≠ Y275), V241 (≠ I277), G248 (= G285), A249 (≠ G286), G250 (= G287), N275 (= N307), A279 (≠ F311)

2dcnA Crystal structure of 2-keto-3-deoxygluconate kinase from sulfolobus tokodaii complexed with 2-keto-6-phosphogluconate (alpha-furanose form)
27% identity, 97% coverage: 2:338/348 of query aligns to 1:305/308 of 2dcnA

query
sites
2dcnA

G

A

K

K

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R

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A

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M

V

E

M

K
|
K

F

L

P

-

R

-

A

-

K

-

V

-

I

-

T

-

T
x
G

L
x
P

R

K

G
|
G

S

A

I

I

S

-

A

-

S

-

H

-

N

-

T

-

W

-

A

-

G

-

V

V

L

Y

Y

Y

D

D

G

G

K

K

T

K

L

Y

F

Y

Q

S

T

S

R

-

Q

G

Y

Y

Q

Q

I

V

T

P

D

-

I
x
V

V

E

D

D

R

V

V
x
T

G
|
G

G
x
A

G
|
G

D
|
D

S

A

F

-

M

L

G

G

L

T

I

F

Y

L

G

S

L

L

L

Y

K

Y

Y

K

P

G

K

F

D

E

D

M

Q

E

N

K

A

A

L

L

D

D

F

Y

A

A

V
x
I

A

V

A

A

S
|
S

C

T

L

L

K

N

H
x
V

T

M

I

I

K

R

G

G

D
|
D

A

Q

-

E

N

N

L

L

V

P

T

T

V

T

A

K

E

D

V

I

E

E

K

T

L

F

M

L

active site: G252 (= G285), A253 (≠ G286), G254 (= G287), D255 (= D288)
binding adenosine-5'-diphosphate: D193 (≠ N198), K223 (= K237), G225 (≠ T247), P226 (≠ L248), G228 (= G250), V247 (≠ I280), G254 (= G287), I279 (≠ V313), S282 (= S316), V286 (≠ H320)
binding 6-O-phosphono-beta-D-psicofuranosonic acid: G33 (= G34), F89 (≠ Y89), Y105 (= Y104), R107 (= R106), I136 (= I136), R165 (= R169), T251 (≠ V284), G252 (= G285), D255 (= D288), D291 (= D325)

Q97U29 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase; 2-dehydro-3-deoxyglucono/galactono-kinase; 2-keto-3-deoxy-galactonokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; KDGal kinase; EC 2.7.1.178 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
24% identity, 97% coverage: 1:339/348 of query aligns to 1:309/313 of Q97U29

query
sites
Q97U29

M

M

G

V

K

D

V

V

V

I

T

A

F

L

G

G

E

E

I

P

M

L

L

I

R

Q

L

F

A

N

P

S

-

F

-

N

Q

P

G

G

F

P

L

L

R

R

F

F

S

-

Q

-

A

V

N

N

S

Y

F

F

D

E

A

K

I

H

Y

V

G

A

G
|
G

G
x
S

E
|
E

S
x
L

N
|
N

V

F

A

C

V

I

S

A

L

V

A

V

N

R

Y

N

G

H

V

L

D

S

V

C

D

S

F

L

V

I

T

A

R

R

L

V

P

G

K

N

N

D

D

E

I

F

G

G

E

K

C

N

A

I

M

I

M

E

E

Y

M

S

R

R

K

A

R

Q

G

G

V

I

G

D

V

T

D

S

K

H

I

I

V

K

W

V

G

D

G

N

D

E

R

S

L

F

-

T

G

G

I

I

Y
|
Y

F

F

L

I

E

Q

T

R

G

G

-

Y

A

P

V

I

S

P

R

M

G

K

S

S

K

E

V

L

V

V

Y
|
Y

D
x
Y

R
|
R

A

K

H

G

S

S

A

A

I

G

A

S

E

R

I

L

K

S

S

P

G

E

M

D

I

I

D

N

W

E

D

N

A

Y

V

V

F

-

E

R

G

N

C

S

E

R

W

L

F

V

H

H

W

S

T

T

G

G

I

I

T

T

P

L

A

A

I

I

S

S

Q

D

G

N

S

A

A

K

D

E

V

A

C

V

L

I

E

K

A

A

V

F

K

E

A

L

A

A

S

K

A

S

K

R

G

-

I

-

T

-

I

-

S

S

T

L

D

D

L

T

N
|
N

Y
x
I

R
|
R

A

P

K

K

L

L

W

W

K

S

Y

S

C

L

D

E

A

K

A

A

H

-

R

K

E

E

K

T

V

I

M

L

T

S

E

I

L

L

T

K

S

K

Y

Y

-

D

C

I

D

E

I

V

V

L

L

I

G

T

N

D

E

P

E

D

D

D

A

T

E

K

M

I

H

L

F

L

G

D

I

V

K

T

-

D

P

P

E

D

G

-

I

-

T

-

V

-

Q

-

T

-

Q

-

G

-

H

-

D

-

V

-

K

-

A

-

E

E

A

A

F

Y

L

R

S

K

V

Y

C

K

E

E

Q

L

M

G

M

V

E

-

K

-

F

-

P

-

R

-

A

-

K

K

K

V

V

L

I

L

T

Y

T
x
K

L
|
L

-
x
G

-
x
S

R
x
K

G
|
G

S

A

I

I

S

A

A

Y

S

K

H

D

N

N

T

V

W

K

A

A

G

-

V

-

L

-

Y

-

D

-

G

-

K

-

T

-

L

-

F

F

Q

K

T

D

R

-

Q

A

Y

Y

Q

K

I

V

T

P

D

-

I

V

V

E

D

D

R

P

V

T

G
|
G

G
x
A

G
|
G

D
|
D

S

A

F

-

M

M

G

A

G

G

L

T

I

F

Y

V

G

S

L

L

L

Y

K

L

Y

Q

P

G

K

K

D

D

D

I

Q

E

N

Y

A

S

L

L

D

A

F

H

A

G

V

I

A

A

S

S

C

T

L

L

K

V

H

I

T

T

I

V

K

R

G

G

D
|
D

A

N

N

E

L

L

V

T

-

P

T

T

V

L

A

E

E

D

V

A

E

E

K

R

L

F

M

L

G

N

GSELN 34:38 (≠ GGESN 34:38) binding substrate
Y90 (= Y89) binding substrate
YYR 106:108 (≠ YDR 104:106) binding substrate
NIR 164:166 (≠ NYR 167:169) binding ATP
R166 (= R169) binding substrate
KLGSKG 226:231 (≠ TL--RG 247:250) binding ATP
GAGD 255:258 (≠ GGGD 285:288) binding ATP
D258 (= D288) binding substrate
D294 (= D325) binding substrate

2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
24% identity, 97% coverage: 4:339/348 of query aligns to 3:308/311 of 2varA

query
sites
2varA

V

V

V

I

T

A

F

L

G

G

E

E

I

P

M
x
L

L

I

R

Q

L

F

A

N

P

S

-

F

-

N

Q

P

G

G

F

P

L

L

R

R

F

F

S

-

Q

-

A

V

N

N

S

Y

F

F

D

E

A

K

I

H

Y

V

G

A

G
|
G

G
x
S

E

E

S

L

N

N

V

F

A

C

V

I

S

A

L

V

A

V

N

R

Y

N

G

H

V

L

D

S

V

C

D

S

F

L

V

I

T

A

R

R

L

V

P

G

K

N

N

D

D

E

I

F

G

G

E

K

C

N

A

I

M

I

M

E

E

Y

M

S

R

R

K

A

R

Q

G

G

V

I

G

D

V

T

D

S

K

H

I

I

V

K

W

V

G

D

G

N

D

E

R

S

L

F

-

T

G

G

I

I

Y
|
Y

F

F

L

I

E

Q

T

R

G

G

-

Y

A

P

V

I

S

P

R

M

G

K

S

S

K

E

V
x
L

V

V

Y
|
Y

D

Y

R
|
R

A

K

H

G

S

S

A

A

I

G

A

S

E

R

I

L

K

S

S

P

G

E

M

D

I

I

D

N

W

E

D

N

A

Y

V

V

F

-

E

R

G

N

C

S

E

R

W

L

F

V

H

H

W

S

T

T

G

G

I
|
I

T

T

P

L

A

A

I

I

S

S

Q

D

G

N

S

A

A

K

D

E

V

A

C

V

L

I

E

K

A

A

V

F

K

E

A

L

A

A

S

K

A

S

K

R

G

-

I

-

T

-

I

-

S

S

T

L

D

D

L

T

N

N

Y

I

R
|
R

A

P

K

K

L

L

W

W

K

S

Y

S

C

L

D

E

A

K

A

A

H

-

R

K

E

E

K

T

V

I

M

L

T

S

E

I

L

L

T

K

S

K

Y

Y

-

D

C

I

D

E

I

V

V

L

L

I

G

T

N

D

E

P

E

D

D

D

A

T

E

K

M

I

H

L

F

L

G

D

I

V

K

T

-

D

P

P

E

D

G

-

I

-

T

-

V

-

Q

-

T

-

Q

-

G

-

H

-

D

-

V

-

K

-

A

-

E

E

A

A

F

Y

L

R

S

K

V

Y

C

K

E

E

Q

L

M

G

M

V

E

-

K

-

F

-

P

-

R

-

A

-

K

K

K

V

V

L

I

L

T

Y

T
x
K

L

L

-
x
G

-
x
S

R

K

G
|
G

S

A

I

I

S

A

A

Y

S

K

H

D

N

N

T

V

W

K

A

A

G

-

V

-

L

-

Y

-

D

-

G

-

K

-

T

-

L

-

F

F

Q

K

T

D

R

-

Q

A

Y

Y

Q

K

I

V

T

P

D

-

I

V

V

E

D

D

R

P

V
x
T

G
|
G

G
x
A

G
|
G

D
|
D

S

A

F

-

M
|
M

G

A

G

G

L

T

I

F

Y

V

G

S

L

L

L

Y

K

L

Y

Q

P

G

K

K

D

D

D

I

Q

E

N

Y

A

S

L

L

D

A

F

H

A

G

V
x
I

A

A

S
|
S

C

T

L

L

K

V

H
x
I

T

T

I

V

K

R

G

G

D
|
D

A

N

N

E

L

L

V

T

-

P

T

T

V

L

A

E

E

D

V

A

E

E

K

R

L

F

M

L

G

N

active site: G254 (= G285), A255 (≠ G286), G256 (= G287), D257 (= D288)
binding adenosine monophosphate: G227 (vs. gap), G230 (= G250), M259 (= M291), S284 (= S316), I288 (≠ H320)
binding phosphoaminophosphonic acid-adenylate ester: K225 (≠ T247), G227 (vs. gap), S228 (vs. gap), G230 (= G250), G254 (= G285), A255 (≠ G286), G256 (= G287), D257 (= D288), M259 (= M291), I281 (≠ V313), S284 (= S316), I288 (≠ H320)
binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: L10 (≠ M11), G33 (= G34), S34 (≠ G35), Y89 (= Y89), Y105 (= Y104), R107 (= R106), I136 (= I136), R165 (= R169), G254 (= G285), D257 (= D288)
binding 2-keto-3-deoxygluconate: G33 (= G34), S34 (≠ G35), Y89 (= Y89), L103 (≠ V102), Y105 (= Y104), R107 (= R106), I136 (= I136), R165 (= R169), T253 (≠ V284), G254 (= G285), D257 (= D288), D293 (= D325)

3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
22% identity, 85% coverage: 4:298/348 of query aligns to 3:256/304 of 3ih0A

query
sites
3ih0A

V

I

V

A

T

S

F

I

G

G

E

E

I

L

M

L

L

I

R

D

L

L

A

I

P

S

Q

V

G

E

F

E

L

G

R

D

F

L

S

K

Q

D

A

V

N

R

S

L

F

F

D

E

A

K

I

H

Y

P

G

G

G

A

E

P

S

A

N

N

V

V

A

A

V

V

S

G

L

V

A

S

N

R

Y

L

G

G

V

V

D

K

V

S

D

S

F

L

V

I

T

S

R

K

L

V

P

G

K

N

N

D

D

P

I

F

G

G

E

E

C

Y

A

L

M

I

M

E

E

E

M

L

R

S

K

K

R

E

G

N

V

V

G

D

V

T

D

R

K

G

I

I

V

V

W

K

G

D

G

E

D

K

R

K

-

H

L

T

G

G

I

I

Y

V

F

F

L

V

E

Q

T

L

G

K

A

G

V

A

S

S

R

-

G

P

S

S

K

F

V

L

Y

Y

D

D

R

D

A

V

H

-

S

-

A

A

I

Y

A

F

E

N

I

M

K

T

S

L

G

N

M

D

I

I

D

N

W

W

D

D

A

I

V

V

F

-

E

E

G

E

C

A

E

K

W

I

F

V

H

N

W

F

T

G

G

S

I

V

T

I

P

L

A

A

I

-

S

R

Q

N

G

P

S

S

A

R

D

E

V

T

C

V

L

M

E

K

A

V

V

I

K

K

A

K

A

I

S

K

A

G

K

S

G

S

I

L

T

-

I

I

S

A

T

F

D

D

L

V

N

N

Y

L

R

R

A

L

K

D

L

L

W

W

K

R

Y

-

C

G

D

Q

A

E

H

E

R

M

E

I

K

K

V

V

M

L

T

E

E

E

L

S

T

I

S

K

Y

L

C

A

D

D

I

I

V

V

L

K

G

A

N

S

E

E

D

-

A

-

E

-

M

-

H

-

F

-

G

-

I

-

K

-

P

-

E

E

G

V

I

L

T

Y

V

L

Q

E

T

N

Q

Q

G

G

H

V

D

E

V

V

K

K

A

G

E

S

A

M

F

L

L

T

S

A

V

I

C
x
T

E

L

Q
x
G

M

-

E

-

K

-

F

-

P

P

R

K

A

G

K

F

K

R

V

L

I

I

T

K

T

N

L

-

R

-

G

-

S

-

I

-

S

-

A

-

S

-

H

-

N

-

T

-

W

-

A

-

G

-

V

-

L

-

Y

-

D

-

G

-

K

E

T

T

L

V

F

V

Q

D

T

V

R

P

Q

S

Y

Y

Q

N

I
x
V

T

N

D

P

I

L

V

-

D

D

R

T

V

T

G
|
G

G
x
A

G
|
G

D
|
D

S

A

F

F

M

M

G

A

L

L

I

L

Y

V

G

G

L

I

active site: G243 (= G285), A244 (≠ G286), G245 (= G287), D246 (= D288)
binding phosphoaminophosphonic acid-adenylate ester: T214 (≠ C231), G216 (≠ Q233), V236 (≠ I277), A244 (≠ G286), G245 (= G287)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 275

3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
22% identity, 85% coverage: 4:298/348 of query aligns to 2:255/302 of 3gbuA

query
sites
3gbuA

V

I

V

A

T

S

F

I

G

G

E

E

I

L

M

L

L

I

R

D

L

L

A

I

P

S

Q

V

G

E

F

E

L

G

R

D

F

L

S

K

Q

D

A

V

N

R

S

L

F

F

D

E

A

K

I

H

Y

P

G

G

G

A

E

P

S

A

N

N

V

V

A

A

V

V

S

G

L

V

A

S

N

R

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|
G

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A

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|
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|
D

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active site: G242 (= G285), A243 (≠ G286), G244 (= G287), D245 (= D288)
binding adenosine-5'-triphosphate: K188 (≠ L196), T213 (≠ C231), G215 (≠ Q233), V235 (≠ I277), P237 (≠ D279), A243 (≠ G286), G244 (= G287)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 274

Query Sequence

>WP_019388253.1 NCBI__GCF_000283015.1:WP_019388253.1
MGKVVTFGEIMLRLAPQGFLRFSQANSFDAIYGGGESNVAVSLANYGVDVDFVTRLPKND
IGECAMMEMRKRGVGVDKIVWGGDRLGIYFLETGAVSRGSKVVYDRAHSAIAEIKSGMID
WDAVFEGCEWFHWTGITPAISQGSADVCLEAVKAASAKGITISTDLNYRAKLWKYCDAAH
REKVMTELTSYCDIVLGNEEDAEMHFGIKPEGITVQTQGHDVKAEAFLSVCEQMMEKFPR
AKKVITTLRGSISASHNTWAGVLYDGKTLFQTRQYQITDIVDRVGGGDSFMGGLIYGLLK
YPKDDQNALDFAVAASCLKHTIKGDANLVTVAEVEKLMGGDASGRVAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory