SitesBLAST

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 40% coverage: 62:166/261 of query aligns to 69:180/203 of P07464

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P07464

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S71 (≠ T64) binding substrate
N85 (≠ D81) binding in other chain; binding in other chain
D93 (≠ G89) binding substrate
H115 (≠ W111) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A128) binding in other chain
A160 (≠ S146) binding in other chain
TK 165:166 (≠ VK 151:152) binding acetyl-CoA
R180 (= R166) binding acetyl-CoA

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
183 binding in other chain

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
28% identity, 40% coverage: 62:166/261 of query aligns to 68:179/201 of 1krvA

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1krvA

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binding 4-nitrophenyl beta-D-galactopyranoside: S70 (≠ T64), Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), M126 (≠ R116)
binding coenzyme a: A104 (≠ Y101), S110 (≠ G107), T112 (= T109), W138 (≠ V125), G140 (= G127), S141 (≠ A128), N146 (≠ L133), A159 (≠ S146), P177 (= P164), R179 (= R166)

Sites not aligning to the query:

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
28% identity, 40% coverage: 62:166/261 of query aligns to 68:179/201 of 1kruA

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binding coenzyme a: H114 (≠ W111), W138 (≠ V125), G140 (= G127), S141 (≠ A128), G158 (= G145), A159 (≠ S146), P177 (= P164)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: S70 (≠ T64), Y82 (≠ F79), Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), L102 (≠ T99), H114 (≠ W111), M126 (≠ R116)

Sites not aligning to the query:

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
28% identity, 40% coverage: 62:166/261 of query aligns to 68:179/200 of 1krrA

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binding acetyl coenzyme *a: N84 (≠ D81), I103 (≠ L100), A104 (≠ Y101), P105 (≠ H102), T112 (= T109), H114 (≠ W111), G140 (= G127), S141 (≠ A128), N146 (≠ L133), G158 (= G145), A159 (≠ S146), A174 (≠ V161), P177 (= P164)

Sites not aligning to the query:

binding acetyl coenzyme *a: 182

Query Sequence

>WP_019555820.1 NCBI__GCF_000381085.1:WP_019555820.1
MFEKIRSDVRCVFERDPAARNTFEVLTTYPGLHAIMWYRLTHWLWNKGLKWLARLISMLA
RWFTGVEIHPAAKIGERFFIDHGMGVVIGETAEIGDDCTLYHGVTLGGTSWKTGKRHPTL
GNGVVVGAGAKVLGPIEVGDNARIGSNAVVVKSVSAGKTVVGIPGREVERKGEDLETRRA
KMADKIGFDAYGVGQEMADPVEKAIYSLLDHIQVQDEKLEKMAQALSKIGGETIEMDLPK
LDDVVLEPDDPVQAAHLKSTK

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory