SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_019555837.1 NCBI__GCF_000381085.1:WP_019555837.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
44% identity, 99% coverage: 5:565/567 of query aligns to 99:661/670 of P17597

query
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P17597

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A122 (= A28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E51) binding thiamine diphosphate
S186 (= S93) binding FAD
P197 (≠ A104) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ S106) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q114) binding thiamine diphosphate
K220 (= K127) binding (R)-imazaquin
R246 (= R153) binding (R)-imazaquin; binding FAD
K256 (= K163) binding chlorimuron-ethyl
G308 (= G213) binding FAD
TL 331:332 (= TL 238:239) binding FAD
C340 (≠ A247) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 256:259) binding FAD
GVRFD 371:375 (≠ GARFD 278:282) binding FAD
DR 376:377 (= DR 283:284) binding chlorimuron-ethyl
DI 395:396 (= DI 302:303) binding FAD
DV 414:415 (≠ PV 321:322) binding FAD
QH 487:488 (= QH 395:396) binding thiamine diphosphate
GG 508:509 (= GG 416:417) binding FAD
GAM 511:513 (≠ GTM 419:421) binding thiamine diphosphate
D538 (≠ E446) binding Mg(2+)
DGS 538:540 (≠ EGS 446:448) binding thiamine diphosphate
N565 (= N473) binding Mg(2+)
NQHLGM 565:570 (≠ NGFLGM 473:478) binding thiamine diphosphate
H567 (≠ F475) binding Mg(2+)
W574 (= W482) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A557) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/585 of 5k2oA

query
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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M258), R292 (= R284), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G286 (= G278), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ F475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 7tzzA

query
sites
7tzzA

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I

binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M258), R292 (= R284), W489 (= W482), S568 (≠ A557)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), D453 (≠ E446), G454 (= G447), S455 (= S448), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), M263 (= M255), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), R288 (= R280), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
binding magnesium ion: A37 (= A28), T82 (= T74), S83 (= S75), Q122 (= Q114), Y381 (≠ A374), D453 (≠ E446), M458 (= M451), Q461 (= Q454), N480 (= N473), H482 (≠ F475), K533 (≠ T523)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 8et4A

query
sites
8et4A

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), D453 (≠ E446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ F475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ A279), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
binding magnesium ion: F370 (≠ Y363), D453 (≠ E446), M458 (= M451), Q461 (= Q454), N480 (= N473), H482 (≠ F475), K533 (≠ T523)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M258), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A557)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 5wj1A

query
sites
5wj1A

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), M263 (= M255), L264 (= L256), G286 (= G278), R288 (= R280), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M258), D291 (= D283), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A557)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ F475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 5k6tA

query
sites
5k6tA

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M

I

I

P

P

A
x
S

G

G

A

G

G

T

L

F

N

N

E

D

M

V

I

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H259), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G286 (= G278), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ F475), L483 (= L476), G484 (= G477)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 5k6rA

query
sites
5k6rA

G

G

A

A

Q

D

I

I

L

L

V

V

N

E

Y

A

L

L

E

E

D

R

E

Q

G

G

V

V

E

E

H

T

I

V

W

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

L

M

P

E

I

I

Y

H

D

Q

A

A

L

L

D

-

T

T

D

R

A

S

K

S

V

I

S

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

P

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

L

L

V

V

C

A

I

I

T

T

G

G

Q

Q

V

V

A

P

T

R

S

R

L

M

I

I

G

G

L

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

I

T

D

P

T

I

T

V

G

E

I

V

T

T

R

R

P

S

I

I

V

T

K

K

H

H

N

N

F

Y

L

L

V

V

K

M

N

D

V

V

D

E

D

D

L

I

A

P

I

R

T

I

L

I

K

E

A

A

F

F

Y

F

L

L

A

A

T

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

V

V

D

D

I

V

P

P

K
|
K

D

D

V

I

Q

Q

T

Q

A

Q

K

L

S

A

N

I

Y

P

A

N

F

W

P

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Q

Q

E

A

V

M

E

R

M

L

R

P

S

G

Y

Y

L

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-

S

-

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-

M

-

P

-

K

P

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V

P

T

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K

D

G

S

H

H

S

L

G

E

Q

Q

I

I

K

-

A

-

V

V

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R

M

L

M

I

L

S

S

E

A

S

K

K

R

K

P

P

I

V

L

L

Y

Y

T

V

G
|
G

V

C

I

L

L

-

G

-

D

N

A

S

S

S

A

D

E

E

L

L

T

G

E

R

L

F

T

V

H

E

K

L

L

T

G

G

F

I

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I

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T

L
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L

M
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M

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F

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P

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C

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D

D

K

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L

G

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

E

Y

A

A

N

N

L

Y

S

A

M

V

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E

H

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S

D

D

L

L

I

L

A

A

I

F

G
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G

A

V

R
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R

F

F

D

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R

V
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V

T

T

G

G

N

K

L

L

E

E

K

A

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F

C

A

P

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D

R

A

A

K

K

I

I

I

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H

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I

D
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D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

V

K

L

T

V

P

D

H

I

V

P

S

I

V

V

C

G
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G

P
x
D

V
|
V

K

K

Q

L

V

A

V

L

T

Q

E

G

M

M

L

N

A

K

N

V

L

L

E

E

K

N

T

R

-

A

-

E

K

E

L

L

K

K

S

L

D

D

E

-

K

-

A

-

L

F

A

G

A

V

W

W

W

R

A

N

Q

E

I

L

E

N

E

V

W

Q

R

K

A

Q

T

K

E

F

C

P

L

L

R

S

Y

F

D

K

T

T

M

F

G

G

A

E

K

A

I

I

K

P

P

P

Q

Q

A

Y

A

A

V

I

E

K

A

V

V

L

W

D

K

E

A

L

T

T

K

D

G

G

D

K

A

A

Y

I

V

I

S

S

S

T

D

G

V
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V

G
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G

Q
|
Q

H
|
H

Q

Q

M
|
M

F

W

A

A

Q

Q

Y

F

Y

Y

P

N

F

Y

D

K

K

K

P

P

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R

R

Q

W

W

L

L

N

S

S

S

G
|
G

G

G

L

L

G
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G

T

A

M
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M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

A

S

L

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A

A

F

N

P

P

D

D

E

A

I

I

I

V

V

V

C

D

V

I

T

D

G

G

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x
D

G
|
G

S
|
S

I

F

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I

M
|
M

N

N

I

V

Q

Q

E

E

L

L

S

A

T

T

C

I

L

R

Q

V

Y

E

G

N

F

L

A

P

I

V

K

K

I

V

I

L

C

L

L

L

N

N

N
|
N

G

Q

F
x
H

L
|
L

G
|
G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

F

R

F

F

Y

Y

E

K

R

A

R

N

Y

R

S

A

M

H

S

T

Y

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

S

I

L

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

Y

C

G

G

H

I

V

P

G

A

V

A

R

R

I

V

D

-

N

-

P

-

K

-

T

T

M

K

T

K

A

A

Q

D

L

L

D

R

E

E

V

A

F

I

S

Q

E

T

K

M

Y

L

K

D

D

T

R

P

L

G

V

P

F

Y

-

L

V

L

D

D

I

V

I

I

T

C

D

P

Q
x
H

Q

Q

E

E

N

H

V

V

Y

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

G

T

L

F

N

N

E

D

M

V

I

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R284), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), G286 (= G278), R288 (= R280), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), G328 (= G320), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), M458 (= M451), N480 (= N473), H482 (≠ F475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 1z8nA

query
sites
1z8nA

G

G

A

A

Q

D

I

I

L

L

V

V

N

E

Y

A

L

L

E

E

D

R

E

Q

G

G

V

V

E

E

H

T

I

V

W

F

G

A

Y
|
Y

P

P

G
|
G

A
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A

V
x
S

L

M

P

E

I

I

Y

H

D

Q

A

A

L

L

D

-

T

T

D

R

A

S

K

S

V

I

S

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

P

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

L

L

V

V

C

A

I

I

T

T

G

G

Q

Q

V

V

A

P

T

R

S

R

L

M

I

I

G

G

L

T

D

D

A

A

F
|
F

Q
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Q

E
|
E

I

T

D

P

T

I

T

V

G

E

I

V

T

T

R

R

P

S

I

I

V

T

K
|
K

H

H

N

N

F

Y

L

L

V

V

K

M

N

D

V

V

D

E

D

D

L

I

A

P

I

R

T

I

L

I

K

E

A

A

F

F

Y

F

L

L

A

A

T

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

V

V

D

D

I

V

P

P

K
|
K

D

D

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I

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Q

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Y

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Y

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R

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-

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M

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K

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L

Y

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G
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G

G

G

G
|
G

V

C

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L

-

G

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D

N

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M

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L

G
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G

M
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M

H
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H

G

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Y

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A

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N

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G

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x
V

R
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R

F

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R
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V
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V

T

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G

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I
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I

D
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D

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A

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I

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G

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S

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M

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N

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Y

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N

F

L

A

P

I

V

K

K

I

V

I

L

C

L

L

L

N

N

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N

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x
H

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L

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G

M
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M

V
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V

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W

Q

E

E

D

F

R

F

F

Y

Y

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K

R

A

R

N

Y

R

S

A

M

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S

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F

M

L

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G

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D

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P

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A

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Q

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E

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D

E

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S

I

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P

P

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N

F

M

V

L

K

L

L

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A

A

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A

S

A

Y

C

G

G

H

I

V

P

G

A

V

A

R

R

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D

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N

-

P

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K

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T

T

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K

T

K

A

A

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L

L

D

R

E

E

V

A

F

I

S

Q

E

T

K

M

Y

L

K

D

D

T

R

P

L

G

V

P

F

Y

-

L

V

L

D

D

I

V

I

I

T

C

D

P

Q
x
H

Q

Q

E

E

N

H

V

V

Y

L

P

P

M

M

I

I

P

P

A

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G
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G

A

G

G

T

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F

N

N

E

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I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K127), R161 (= R153), Y191 (= Y183), R194 (vs. gap), D291 (= D283), R292 (= R284), D312 (= D304), W489 (= W482), G569 (= G558)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ A279), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473)
binding thiamine diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), G452 (= G445), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ F475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 1yi1A

query
sites
1yi1A

G

G

A

A

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I

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-

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T

S

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G

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G

G

A

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T

N

N

A

L

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G

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D

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E

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K

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I

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F

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L

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A

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-

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E

T

K

M

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L

K

D

D

T

R

P

L

G

V

P

F

Y

-

L

V

L

D

D

I

V

I

I

T

C

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P

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x
H

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E

E

N

H

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V

Y

L

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P

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M

I

I

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x
S

G

G

A

G

G

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F

N

N

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D

M

V

I

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D283), R292 (= R284), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), M263 (= M255), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ A279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 1yi0A

query
sites
1yi0A

G

G

A

A

Q

D

I

I

L

L

V

V

N

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Y

A

L

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L

I

V

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T

S

S

G

G

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P

G

G

A

A

T

T

N

N

A

L

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V

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G

G

I

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A

A

T

D

A

A

Y

L

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G

E

I

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K

K

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H

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D

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R

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K

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D

D

T

R

P

L

G

V

P

F

Y

-

L

V

L

D

D

I

V

I

I

T

C

D

P

Q
x
H

Q

Q

E

E

N

H

V

V

Y

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

G

T

L

F

N

N

E

D

M

V

I

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D283), R292 (= R284), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ A279), R288 (= R280), D290 (= D282), R292 (= R284), V293 (= V285), D310 (= D302), I311 (= I303), G328 (= G320), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 1yhzA

query
sites
1yhzA

G

G

A

A

Q

D

I

I

L

L

V

V

N

E

Y

A

L

L

E

E

D

R

E

Q

G

G

V

V

E

E

H

T

I

V

W

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

L

M

P

E

I

I

Y

H

D

Q

A

A

L

L

D

-

T

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A

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K

S

V

I

S

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H

N

I

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L

L

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R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

P

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

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D

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L

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V

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A

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I

T

T

G

G

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Q

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V

A

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S

R

L

M

I

I

G

G

L

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D

D

A

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F
|
F

Q
|
Q

E
|
E

I

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D

P

T

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G

E

I

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T

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R

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I

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K

K

H

H

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N

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L

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D

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D

D

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L

I

K

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A

A

F

F

Y

F

L

L

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G

G

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|
R

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D

D

I

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|
K

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|
G

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L

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|
T

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L

M
|
M

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G

L

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F

Y

P

P

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C

S

D

D

D

K

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S

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G

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

Y

V

E

Y

A

A

N

N

L

Y

S

A

M

V

H

E

H

H

S

S

D

D

L

L

I

L

A

A

I

F

G
|
G

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x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

N

K

L

L

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F

F

C

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D

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A

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K

K

I

I

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|
D

I
|
I

D

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A

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I

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K

K

N

N

V

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I

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C

G

G

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D

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|
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K

K

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|
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|
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|
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|
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|
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-

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L

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H

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L

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M

M

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I

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A
x
S

G

G

A

G

G

T

L

F

N

N

E

D

M

V

I

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D283), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ A279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 1yhyA

query
sites
1yhyA

G

G

A

A

Q

D

I

I

L

L

V

V

N

E

Y

A

L

L

E

E

D

R

E

Q

G

G

V

V

E

E

H

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I

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W

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G

A

Y
|
Y

P

P

G
|
G

A
|
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V
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M

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I

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H

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A

A

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L

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T

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A

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K

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N

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L

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|
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Q

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A

D

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G

G

Y

Y

A

A

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R

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S

T

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G

K

K

P

P

G

G

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I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

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N

N

A

L

V

V

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G

I

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A

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|
R

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D

M

V

I

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D283), R292 (= R284), V486 (= V479), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), G265 (= G257), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ A279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/582 of 1ybhA

query
sites
1ybhA

G

G

A

A

Q

D

I

I

L

L

V

V

N

E

Y

A

L

L

E

E

D

R

E

Q

G

G

V

V

E

E

H

T

I

V

W

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

L

M

P

E

I

I

Y

H

D

Q

A

A

L

L

D

-

T

T

D

R

A

S

K

S

V

I

S

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

P

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

L

L

V

V

C

A

I

I

T

T

G

G

Q

Q

V

V

A

P

T

R

S

R

L

M

I

I

G

G

L

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

I

T

D

P

T

I

T

V

G

E

I

V

T

T

R

R

P

S

I

I

V

T

K

K

H

H

N

N

F

Y

L

L

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V

K

M

N

D

V

V

D

E

D

D

L

I

A

P

I

R

T

I

L

I

K

E

A

A

F

F

Y

F

L

L

A

A

T

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

V

V

D

D

I

V

P

P

K
|
K

D

D

V

I

Q

Q

T

Q

A

Q

K

L

S

A

N

I

Y

P

A

N

F

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Q

Q

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M

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M

L

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Y

Y

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S

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K

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Q

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A

-

V

V

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M

L

M

I

L

S

S

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A

S

K

K

R

K

P

P

I

V

L

L

Y

Y

T

V

G

G

G
|
G

V

C

I

L

L

-

G

-

D

N

A

S

S

S

A

D

E

E

L

L

T

G

E

R

L

F

T

V

H

E

K

L

L

T

G

G

F

I

P

P

I

V

T

A

Q

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

F

Y

P

P

A

C

S

D

D

D

K

E

Q

L

S

S

V

L

G

H

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

T

Y

V

E

Y

A

A

N

N

L

Y

S

A

M

V

H

E

H

H

S

S

D

D

L

L

I

L

A

A

I

F

G
|
G

A
x
V

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

N

K

L

L

E

E

K

A

F

F

C

A

P

S

D

R

A

A

K

K

I

I

I

V

H

H

V

I

D
|
D

I
|
I

D

D

P

S

A

A

S

E

I

I

S

G

K

K

N

N

V

K

L

T

V

P

D

H

I

V

P

S

I

V

V

C

G

G

P
x
D

V
|
V

K

K

Q

L

V

A

V

L

T

Q

E

G

M

M

L

N

A

K

N

V

L

L

E

E

K

N

T

R

-

A

-

E

K

E

L

L

K

K

S

L

D

D

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-

K

-

A

-

L

F

A

G

A

V

W

W

W

R

A

N

Q

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I

L

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N

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W

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A

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C

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L

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Y

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D

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G

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A

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I

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P

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Q

A

Y

A

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I

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K

A

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V

L

W

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K

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A

L

T

T

K

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G

G

D

K

A

A

Y

I

V

I

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S

S

T

D

G

V
|
V

G

G

Q

Q

H

H

Q
|
Q

M
|
M

F

W

A

A

Q

Q

Y

F

Y

Y

P

N

F

Y

D

K

K

K

P

P

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R

R

Q

W

W

L

L

N

S

S

S

G
|
G

L

L

G
|
G

T

A

M
|
M

G

G

F

F

G

G

L

L

P

P

A

A

M

I

G

G

A

A

A

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L

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A

A

F

N

P

P

D

D

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A

I

I

I

V

V

V

C

D

V

I

T

D

G

G

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D

G

G

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S

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F

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M

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N

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L

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T

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I

L

R

Q

V

Y

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G

N

F

L

A

P

I

V

K

K

I

V

I

L

C

L

L

L

N

N

N
|
N

G

Q

F
x
H

L
|
L

G

G

M
|
M

V
|
V

R

M

Q

Q

W
|
W

Q

E

E

D

F

R

F

F

Y

Y

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K

R

A

R

N

Y

R

S

A

M

H

S

T

Y

F

M

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

E

E

S

I

L

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

Y

C

G

G

H

I

V

P

G

A

V

A

R

R

I

V

D

-

N

-

P

-

K

-

T

T

M

K

T

K

A

A

Q

D

L

L

D

R

E

E

V

A

F

I

S

Q

E

T

K

M

Y

L

K

D

D

T

R

P

L

G

V

P

F

Y

-

L

V

L

D

D

I

V

I

I

T

C

D

P

Q
x
H

Q

Q

E

E

N

H

V

V

Y

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

G

T

L

F

N

N

E

D

M

V

I

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M258), D291 (= D283), R292 (= R284), M485 (= M478), W489 (= W482), S568 (≠ A557)
binding flavin-adenine dinucleotide: R161 (= R153), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), H267 (= H259), G286 (= G278), V287 (≠ A279), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), Q404 (= Q397), M405 (= M398), G423 (= G416), G424 (= G417)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 14:576/583 of 5k3sA

query
sites
5k3sA

G

G

A

A

Q

D

I

I

L

L

V

V

N

E

Y

A

L

L

E

E

D

R

E

Q

G

G

V

V

E

E

H

T

I

V

W

F

G

A

Y
|
Y

P

P

G
|
G

A
|
A

V
x
S

L

M

P

E

I

I

Y

H

D

Q

A

A

L

L

D

-

T

T

D

R

A

S

K

S

V

I

S

R

H

N

I

V

L

L

V

P

R

R

H

H

E
|
E

Q

Q

A

G

V

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

S

S

T

S

G

G

K

K

P

P

G

G

V

I

V

C

L

I

V

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

V

V

T

S

G

G

I

L

A

A

T

D

A

A

Y

L

M

L

D

D

S

S

I

V

P

P

L

L

V

V

C

A

I

I

T

T

G

G

Q

Q

V

V

A

P

T

R

S

R

L

M

I

I

G

G

L

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

I

T

D

P

T

I

T

V

G

E

I

V

T

T

R

R

P

S

I

I

V

T

K

K

H

H

N

N

F

Y

L

L

V

V

K

M

N

D

V

V

D

E

D

D

L

I

A

P

I

R

T

I

L

I

K

E

A

A

F

F

Y

F

L

L

A

A

T

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

V

V

D

D

I

V

P

P

K
|
K

D

D

V

I

Q

Q

T

Q

A

Q

K

L

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A

N

I

Y

P

A

N

F

W

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Q

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A

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M

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R

M

L

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S

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Y

Y

L

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-

S

-

R

-

M

-

P

-

K

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V

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K

D

G

S

H

H

S

L

G

E

Q

Q

I

I

K

-

A

-

V

V

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M

L

M

I

L

S

S

E

A

S

K

K

R

K

P

P

I

V

L

L

Y

Y

T

V

G
|
G

V

C

I

L

L

-

G

-

D

N

A

S

S

S

A

D

E

E

L

L

T

G

E

R

L

F

T

V

H

E

K

L

L

T

G

G

F

I

P

P

I

V

T

A

Q

S

T
|
T

L
|
L

M
|
M

G

G

L

L

G

G

A

S

F

Y

P

P

A

C

S

D

D

D

K

E

Q

L

S

S

V

L

G

H

M

M

L
|
L

G

G

M
|
M

H

H

G

G

T

T

Y

V

E

Y

A

A

N

N

L

Y

S

A

M

V

H

E

H

H

S

S

D

D

L

L

I

L

A

A

I

F

G
|
G

A

V

R
|
R

F

F

D
|
D

D

D

R
|
R

V
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V

T

T

G

G

N

K

L

L

E

E

K

A

F

F

C

A

P

S

D

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A

A

K

K

I

I

I

V

H

H

V

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D
|
D

I
|
I

D

D

P

S

A

A

S

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I

I

S

G

K

K

N

N

V

K

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D

H

I

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P

S

I

V

V

C

G

G

P
x
D

V
|
V

K

K

Q

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M

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A

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G
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G

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Q

H
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H

Q

Q

M
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M

F

W

A

A

Q

Q

Y

F

Y

Y

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N

F

Y

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K

K

K

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W

L

L

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S

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G
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G

G

G

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L

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M

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K

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N

N
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N

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H

L
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L

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M
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M

V
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|
W

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L

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L

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D

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H

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Q

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N

H

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V

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M

M

I

I

P

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A

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G
|
G

A

G

G

T

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F

N

N

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D

M

V

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I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (= A28), S38 (≠ V29), E59 (= E51), T82 (= T74), F121 (= F113), Q122 (= Q114), E123 (= E115), K171 (= K163), M266 (= M258), V293 (= V285), V400 (= V393), G426 (= G419), M428 (= M421), D453 (≠ E446), N480 (= N473), H482 (≠ F475), L483 (= L476), M485 (= M478), V486 (= V479), W489 (= W482), H558 (≠ Q547)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R284), M485 (= M478), W489 (= W482), G569 (= G558)
binding flavin-adenine dinucleotide: R161 (= R153), G222 (= G212), G223 (= G213), G224 (= G214), T246 (= T238), L247 (= L239), M248 (= M240), L264 (= L256), M266 (= M258), G286 (= G278), R288 (= R280), D290 (= D282), V293 (= V285), D310 (= D302), I311 (= I303), D329 (≠ P321), V330 (= V322), M405 (= M398), G423 (= G416)
binding magnesium ion: D453 (≠ E446), N480 (= N473), H482 (≠ F475)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V393), G401 (= G394), Q402 (= Q395), H403 (= H396), G426 (= G419), M428 (= M421), D453 (≠ E446), G454 (= G447), S455 (= S448), N480 (= N473), H482 (≠ F475), L483 (= L476), G484 (= G477), M485 (= M478), V486 (= V479)

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
43% identity, 99% coverage: 5:565/567 of query aligns to 96:658/667 of P09342

query
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P09342

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C161 (≠ V71) modified: Disulfide link with 307
P194 (≠ A104) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V215) modified: Disulfide link with 161

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 13:575/582 of 3ea4A

query
sites
3ea4A

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active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (= A28), S37 (≠ V29), E58 (= E51), T81 (= T74), F120 (= F113), Q121 (= Q114), E122 (= E115), K170 (= K163), M265 (= M258), V292 (= V285), V399 (= V393), G425 (= G419), M427 (= M421), D452 (≠ E446), N479 (= N473), H481 (≠ F475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (= W482), H557 (≠ Q547)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D283), R291 (= R284), W488 (= W482), S567 (≠ A557)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R153), G221 (= G212), G222 (= G213), G223 (= G214), T245 (= T238), L246 (= L239), M247 (= M240), L263 (= L256), G264 (= G257), M265 (= M258), H266 (= H259), G285 (= G278), R287 (= R280), D289 (= D282), R291 (= R284), D309 (= D302), I310 (= I303), G327 (= G320), D328 (≠ P321), V329 (= V322), M404 (= M398), G422 (= G416)
binding magnesium ion: D452 (≠ E446), N479 (= N473), H481 (≠ F475)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V393), G400 (= G394), Q401 (= Q395), H402 (= H396), M427 (= M421), G451 (= G445), D452 (≠ E446), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ F475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
44% identity, 99% coverage: 5:565/567 of query aligns to 13:575/582 of 3e9yA

query
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active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (= A28), S37 (≠ V29), E58 (= E51), T81 (= T74), F120 (= F113), Q121 (= Q114), E122 (= E115), K170 (= K163), M265 (= M258), V292 (= V285), V399 (= V393), G425 (= G419), M427 (= M421), D452 (≠ E446), N479 (= N473), H481 (≠ F475), L482 (= L476), M484 (= M478), V485 (= V479), W488 (= W482), H557 (≠ Q547)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D283), R291 (= R284), W488 (= W482), S567 (≠ A557)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R153), G221 (= G212), G222 (= G213), G223 (= G214), T245 (= T238), L246 (= L239), M247 (= M240), L263 (= L256), G285 (= G278), R287 (= R280), D289 (= D282), R291 (= R284), D309 (= D302), I310 (= I303), G327 (= G320), D328 (≠ P321), V329 (= V322), M404 (= M398), G422 (= G416)
binding magnesium ion: D452 (≠ E446), N479 (= N473), H481 (≠ F475)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V393), G400 (= G394), Q401 (= Q395), H402 (= H396), M427 (= M421), G451 (= G445), G453 (= G447), S454 (= S448), N479 (= N473), H481 (≠ F475), L482 (= L476), G483 (= G477), M484 (= M478), V485 (= V479)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
43% identity, 99% coverage: 5:565/567 of query aligns to 93:655/664 of P09114

query
sites
P09114

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P191 (≠ A104) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W482) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

1t9dA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
43% identity, 99% coverage: 3:565/567 of query aligns to 8:574/596 of 1t9dA

query
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1t9dA

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E

D

H

T

I

V

W

F

G

G

Y
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Y

P

P

G
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G

A
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A

V
x
I

L

L

P

P

I

V

Y

Y

D

D

A

A

L

I

D

H

T

-

D

N

A

S

K

D

K

K

V

F

S

N

H

F

I

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L

L

V

P

R

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H

H

E
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E

Q

Q

A

G

A

A

V

G

H

H

A

M

A

A

D

E

G

G

Y

Y

A

A

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R

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A

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S

G

G

K

K

P

P

G

G

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V

L

L

V

V

T
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T

S

S

G

G

P
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P

G

G

A

A

T

T

N

N

A

V

V

V

T

T

G

P

I

M

A

A

T

D

A

A

Y

F

M

A

D

D

S

G

I

I

P

P

L

M

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V

C

V

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F

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T

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G

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V
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x
P

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S

L

A

I

I

G

G

L

T

D

D

A

A

F
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F

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Q

E
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E

I

A

D

D

T

V

G

G

I

I

T

S

R

R

P

S

I

C

V

T

K

K

H

W

N

N

F

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M

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V

K

K

N

S

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V

D

E

L

L

A

P

I

L

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R

L

I

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N

K

E

A

A

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F

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E

L

I

A

A

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T

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R

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P

G

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P

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V

L

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D

I

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K

D

D

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T

T

A

A

A

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I

S

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N

R

Y

N

A

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F

I

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Q

-

E

-

V

-

E

-

M

T

R

K

S

T

Y

T

L

L

P

P

V

T

T

S

K

R

G

A

H

Q

S

D

-

E

-

F

-

V

-

M

G

Q

Q

S

I

I

K

N

K

K

A

A

V

A

E

D

M

L

M

I

L

N

S

L

A

A

K

K

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K

P

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I

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L

L

Y

Y

T

V

G
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G

G
x
A

G
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G

V

I

I

L

-
x
N

L

H

G

A

D

D

A

G

S

P

A
x
R

E

L

L

L

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K

E

E

L

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H

D

K

R

L

A

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Q

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I

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P

I

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T
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T

L
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L

M
x
Q

G

G

L

L

G

G

A

S

F

F

P

D

A

Q

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E

D

D

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P

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M
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M

L
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L

G

G

M
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M

H
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H

G

G

T

C

Y

A

E

T

A

A

N

N

L

L

S

A

M

V

H

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H

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S

A

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D

L

L

I

I

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G

A
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A

R
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R

F

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D
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D

R
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R

V
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V

T

T

G

G

N

N

L

I

E

S

K

K

F

F

C

A

P

P

D

E

A

A

K

R

-

R

-

A

-

E

-

G

-

R

-

G

I

I

H

H

V

F

D
x
E

I
x
V

D

S

P

P

A

K

S
x
N

I

I

S

N

K

K

N

V

V

V

L

Q

V

T

D

Q

I

I

P

A

I

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V

E

G
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G

P
x
D

V
x
A

K

T

Q

T

V

N

V

L

T

G

E

K

M

M

L

M

A

S

N

K

L

I

E

F

K

P

T

V

K

K

L

E

K

R

S

S

D

E

E

-

K

-

A

-

L

-

A

-

W

W

W

F

A

A

Q

Q

I

I

E

N

E

K

W

W

R

K

A

K

T

E

E

Y

C

P

L

Y

R

A

Y

Y

-

M

-

E

D

E

T

T

M

P

G

G

A

S

K

K

I

I

K

K

P

P

Q

Q

A

T

A

V

V

I

E

K

A

K

V

L

W

S

K

K

A

V

-

A

-

N

-

D

T

T

K

G

G

R

D

H

A

V

Y

I

V

V

S

T

D

G

V
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V

G

G

Q

Q

H

H

Q
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Q

M
|
M

F

W

A

A

Q

Q

Y

H

Y

W

P

T

F

W

D

R

K

N

P

P

R

H

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W

F

L

I

N

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S

S

G
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G

L
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L

G
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G

T

T

M
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M

G

G

F

Y

G

G

L

L

P

P

A

A

M

I

G

G

A

A

A

Q

L

V

A

A

F

K

P

P

D

E

E

S

I

L

I

V

V

I

C

D

V

I

T

D

G

G

E
x
D

G

A

S

S

I

F

Q

N

M
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M

N

T

I

L

Q

T

E

E

L

L

S

S

T

S

C

A

L

V

Q

Q

Y

A

G

G

F

T

A

P

I

V

K

K

I

I

I

L

C

I

L

L

N

N

N
|
N

G

E

F
x
E

L
x
Q

G

G

M
|
M

V
|
V

R

T

Q

Q

W
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W

Q

Q

E

S

F

L

F

F

Y

Y

E

E

R

H

R

R

Y

Y

S

S

M

H

S

T

Y

H

M

Q

E

L

S

N

L

-

P

P

D

D

F

F

V

I

K

K

L
|
L

A

A

E

E

S

A

Y

M

G
|
G

H
x
L

V

K

G

G

V

L

R

R

I

V

D

K

N

K

P

Q

K

E

T

E

M

L

T

D

A

A

Q

K

L

L

D

K

E

E

V

F

F

V

S

S

E

T

K

K

Y

G

K

P

D

-

R

-

L

-

V

V

F

L

V

L

D

E

I

V

I

E

T

V

D

D

Q
x
K

Q

K

E

V

N

P

V

V

Y

L

P

P

M

M

I

V

P

A

A

G

G

G

A

S

G

G

L

L

N

D

E

E

M

F

I

I

active site: Y29 (= Y24), G31 (= G26), G32 (= G27), A33 (= A28), I34 (≠ V29), E55 (= E51), T78 (= T74), F117 (= F113), Q118 (= Q114), E119 (= E115), K167 (= K163), R227 (≠ A222), M263 (= M258), V290 (= V285), V406 (= V393), L431 (= L418), G432 (= G419), M434 (= M421), D459 (≠ E446), N486 (= N473), E488 (≠ F475), Q489 (≠ L476), M491 (= M478), V492 (= V479), W495 (= W482), L517 (= L505), G522 (= G510), L523 (≠ H511), K556 (≠ Q547)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G27), A33 (= A28), V107 (= V103), P108 (≠ A104), F117 (= F113), K167 (= K163), M263 (= M258), D288 (= D283), R289 (= R284), W495 (= W482)
binding flavin-adenine dinucleotide: R157 (= R153), G216 (= G212), A217 (≠ G213), G218 (= G214), N221 (vs. gap), T243 (= T238), L244 (= L239), Q245 (≠ M240), M260 (= M255), L261 (= L256), H264 (= H259), G283 (= G278), A284 (= A279), R285 (= R280), D287 (= D282), R289 (= R284), V290 (= V285), E316 (≠ D302), V317 (≠ I303), N321 (≠ S307), G334 (= G320), D335 (≠ P321), A336 (≠ V322), Q410 (= Q397), M411 (= M398), G429 (= G416), G430 (= G417)
binding magnesium ion: D459 (≠ E446), N486 (= N473), E488 (≠ F475)
binding 2,5-dimethyl-pyrimidin-4-ylamine: E55 (= E51), P81 (= P77), Q118 (= Q114), G432 (= G419), M434 (= M421), M464 (= M451)

1t9cA Crystal structure of yeast acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
44% identity, 99% coverage: 3:565/567 of query aligns to 8:574/596 of 1t9cA

query
sites
1t9cA

M

L

T

T

G

G

A

G

Q

Q

I

I

L

F

V

N

N

E

Y

M

L

M

E

S

D

R

E

Q

G

N

V

V

E

D

H

T

I

V

W

F

G

G

Y
|
Y

P

P

G
|
G

A
|
A

V
x
I

L

L

P

P

I

V

Y

Y

D

D

A

A

L

I

D

H

T

-

D

N

A

S

K

D

K

K

V

F

S

N

H

F

I

V

L

L

V

P

R

K

H

H

E
|
E

Q

Q

A

G

A

A

V

G

H

H

A

M

A

A

D

E

G

G

Y

Y

A

A

R

R

S

A

T

S

G

G

K

K

P

P

G

G

V

V

L

L

V

V

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

A

V

V

V

T

T

G

P

I

M

A

A

T

D

A

A

Y

F

M

A

D

D

S

G

I

I

P

P

L

M

V

V

C

V

I

F

T

T

G

G

Q

Q

V
|
V

A
x
P

T

T

S

S

L

A

I

I

G

G

L

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

I

A

D

D

T

V

G

G

I

I

T

S

R

R

P

S

I

C

V

T

K

K

H

W

N

N

F

V

L

M

V

V

K

K

N

S

V

V

D

E

L

L

A

P

I

L

T

R

L

I

K

N

K

E

A

A

F

F

Y

E

L

I

A

A

T

T

T

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

V

V

D

D

I

L

P

P

K
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K

D

D

V

V

Q

T

T

A

A

A

K

I

S

L

N

R

Y

N

A

P

F

I

P

P

Q

T

E

K

V

T

E

T

M

L

R

P

S

S

Y

S

L

R

P

A

V

Q

T

D

K

E

G

F

H

V

S

M

G

Q

Q

S

I

I

K

N

K

K

A

A

V

A

E

D

M

L

M

I

L

N

S

L

A

A

K

K

R

K

P

P

I

V

L

L

Y

Y

T

V

G
|
G

G
x
A

G
|
G

V

I

I

L

-
x
N

L

H

G

A

D

D

A

G

S

P

A
x
R

E

L

L

L

T

K

E

E

L

L

T

S

H

D

K

R

L

A

G

Q

F

I

P

P

I

V

T

T

Q

T

T
|
T

L
|
L

M
x
Q

G

G

L

L

G

G

A

S

F

F

P

D

A

Q

S

E

D

D

K

P

Q

K

S

S

V

L

G

D

M

M

L
|
L

G

G

M
|
M

H
|
H

G

G

T

C

Y

A

E

T

A

A

N

N

L

L

S

A

M

V

H

Q

H

N

S

A

D

D

L

L

I

I

A

A

I

V

G
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G

A
|
A

R
|
R

F

F

D
|
D

R
|
R

V
|
V

T

T

G

G

N

N

L

I

E

S

K

K

F

F

C

A

P

P

D

E

A

A

K

R

-

R

-

A

-

E

-

G

-

R

-

G

I

I

H

H

V

F

D
x
E

I
x
V

D

S

P

P

A

K

S
x
N

I

I

S

N

K

K

N

V

V

V

L

Q

V

T

D

Q

I

I

P

A

I

V

V

E

G
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G

P
x
D

V
x
A

K

T

Q

T

V

N

V

L

T

G

E

K

M

M

L

M

A

S

N

K

L

I

E

F

K

P

T

V

K

K

L

E

K

R

S

S

D

E

E

-

K

-

A

-

L

-

A

-

W

W

W

F

A

A

Q

Q

I

I

E

N

E

K

W

W

R

K

A

K

T

E

E

Y

C

P

L

Y

R

A

Y

Y

-

M

-

E

D

E

T

T

M

P

G

G

A

S

K

K

I

I

K

K

P

P

Q

Q

A

T

A

V

V

I

E

K

A

K

V

L

W

S

K

K

A

V

-

A

-

N

-

D

T

T

K

G

G

R

D

H

A

V

Y

I

V

V

S

T

D

G

V
|
V

G

G

Q

Q

H

H

Q

Q

M
|
M

F

W

A

A

Q

Q

Y

H

Y

W

P

T

F

W

D

R

K

N

P

P

R

H

R

T

W

F

L

I

N

T

S

S

G
|
G

L
|
L

G
|
G

T

T

M
|
M

G

G

F

Y

G

G

L

L

P

P

A

A

M

I

G

G

A

A

A

Q

L

V

A

A

F

K

P

P

D

E

E

S

I

L

I

V

V

I

C

D

V

I

T

D

G

G

E
x
D

G

A

S

S

I

F

Q

N

M

M

N

T

I

L

Q

T

E

E

L

L

S

S

T

S

C

A

L

V

Q

Q

Y

A

G

G

F

T

A

P

I

V

K

K

I

I

I

L

C

I

L

L

N

N

N
|
N

G

E

F
x
E

L
x
Q

G

G

M
|
M

V
|
V

R

T

Q

Q

W
|
W

Q

Q

E

S

F

L

F

F

Y

Y

E

E

R

H

R

R

Y

Y

S

S

M

H

S

T

Y

H

M

Q

E

L

S

N

L

-

P

P

D

D

F

F

V

I

K

K

L
|
L

A

A

E

E

S

A

Y

M

G
|
G

H
x
L

V

K

G

G

V

L

R

R

I

V

D

K

N

K

P

Q

K

E

T

E

M

L

T

D

A

A

Q

K

L

L

D

K

E

E

V

F

F

V

S

S

E

T

K

K

Y

G

K

P

D

-

R

-

L

-

V

V

F

L

V

L

D

E

I

V

I

E

T

V

D

D

Q
x
K

Q

K

E

V

N

P

V

V

Y

L

P

P

M

M

I

V

P

A

A

G

G

G

A

S

G

G

L

L

N

D

E

E

M

F

I

I

active site: Y29 (= Y24), G31 (= G26), G32 (= G27), A33 (= A28), I34 (≠ V29), E55 (= E51), T78 (= T74), F117 (= F113), Q118 (= Q114), E119 (= E115), K167 (= K163), R227 (≠ A222), M263 (= M258), V290 (= V285), V406 (= V393), L431 (= L418), G432 (= G419), M434 (= M421), D459 (≠ E446), N486 (= N473), E488 (≠ F475), Q489 (≠ L476), M491 (= M478), V492 (= V479), W495 (= W482), L517 (= L505), G522 (= G510), L523 (≠ H511), K556 (≠ Q547)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: G32 (= G27), V107 (= V103), P108 (≠ A104), F117 (= F113), K167 (= K163), D288 (= D283), R289 (= R284), W495 (= W482)
binding flavin-adenine dinucleotide: R157 (= R153), G216 (= G212), A217 (≠ G213), G218 (= G214), N221 (vs. gap), T243 (= T238), L244 (= L239), Q245 (≠ M240), L261 (= L256), M263 (= M258), H264 (= H259), G283 (= G278), A284 (= A279), R285 (= R280), D287 (= D282), R289 (= R284), V290 (= V285), E316 (≠ D302), V317 (≠ I303), N321 (≠ S307), G334 (= G320), D335 (≠ P321), A336 (≠ V322), M411 (= M398), G429 (= G416), G430 (= G417)
binding magnesium ion: D459 (≠ E446), N486 (= N473), E488 (≠ F475)

Query Sequence

>WP_019555837.1 NCBI__GCF_000381085.1:WP_019555837.1
MEMTGAQILVNYLEDEGVEHIWGYPGGAVLPIYDALDTDAKKVSHILVRHEQAAVHAADG
YARSTGKPGVVLVTSGPGATNAVTGIATAYMDSIPLVCITGQVATSLIGLDAFQEIDTTG
ITRPIVKHNFLVKNVDDLAITLKKAFYLATTGRPGPVVVDIPKDVQTAKSNYAFPQEVEM
RSYLPVTKGHSGQIKKAVEMMLSAKRPILYTGGGVILGDASAELTELTHKLGFPITQTLM
GLGAFPASDKQSVGMLGMHGTYEANLSMHHSDLIIAIGARFDDRVTGNLEKFCPDAKIIH
VDIDPASISKNVLVDIPIVGPVKQVVTEMLANLEKTKLKSDEKALAAWWAQIEEWRATEC
LRYDTMGAKIKPQAAVEAVWKATKGDAYVSSDVGQHQMFAAQYYPFDKPRRWLNSGGLGT
MGFGLPAAMGAALAFPDEIIVCVTGEGSIQMNIQELSTCLQYGFAIKIICLNNGFLGMVR
QWQEFFYERRYSMSYMESLPDFVKLAESYGHVGVRIDNPKTMTAQLDEVFSEKYKDRLVF
VDIITDQQENVYPMIPAGAGLNEMILV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory