SitesBLAST
Comparing WP_019555908.1 NCBI__GCF_000381085.1:WP_019555908.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5ey5B Lbcats
72% identity, 96% coverage: 13:395/401 of query aligns to 1:383/383 of 5ey5B
- binding pyridoxal-5'-phosphate: H81 (= H93), K82 (= K94), Q109 (= Q121), S185 (≠ T197), G227 (= G239), G229 (= G241), S230 (= S242), N231 (= N243), E345 (= E357), S371 (= S383), G372 (= G384)
5dw0A Trpb from pyrococcus furiosus with l-serine bound as the external aldimine (see paper)
63% identity, 96% coverage: 15:398/401 of query aligns to 3:386/388 of 5dw0A
- active site: K82 (= K94), E104 (= E116), S371 (= S383)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: H81 (= H93), K82 (= K94), T105 (= T117), G106 (= G118), A107 (= A119), Q109 (= Q121), H110 (= H122), S185 (≠ T197), G227 (= G239), G229 (= G241), S230 (= S242), N231 (= N243), G298 (= G310), D300 (= D312), E345 (= E357), S371 (= S383)
5t6mB Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
63% identity, 96% coverage: 15:398/401 of query aligns to 3:386/386 of 5t6mB
1v8zA X-ray crystal structure of the tryptophan synthase b2 subunit from hyperthermophile, pyrococcus furiosus (see paper)
63% identity, 96% coverage: 15:398/401 of query aligns to 3:386/386 of 1v8zA
- active site: K82 (= K94), E104 (= E116), S371 (= S383)
- binding pyridoxal-5'-phosphate: H81 (= H93), K82 (= K94), Q109 (= Q121), S185 (≠ T197), G227 (= G239), G228 (= G240), G229 (= G241), S230 (= S242), N231 (= N243), E345 (= E357), S371 (= S383), G372 (= G384)
6am8B Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9 with trp bound as e(aex2) (see paper)
63% identity, 95% coverage: 15:393/401 of query aligns to 3:381/385 of 6am8B
- active site: K82 (= K94), E104 (= E116), S371 (= S383)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-l-tryptophane: H81 (= H93), K82 (= K94), E104 (= E116), T105 (= T117), G106 (= G118), A107 (= A119), Q109 (= Q121), H110 (= H122), L161 (= L173), S185 (≠ T197), V187 (≠ A199), G227 (= G239), G228 (= G240), G229 (= G241), S230 (= S242), N231 (= N243), G298 (= G310), Y301 (= Y313), E345 (= E357), S371 (= S383), G372 (= G384)
- binding tryptophan: P12 (= P24), L169 (≠ M181), S274 (≠ L286), H275 (= H287)
5dw3A Tryptophan synthase beta-subunit from pyrococcus furiosus with product l-tryptophan non-covalently bound in the active site (see paper)
63% identity, 95% coverage: 15:396/401 of query aligns to 3:383/383 of 5dw3A
- active site: K82 (= K94), E104 (= E116), S370 (= S383)
- binding tryptophan: K82 (= K94), E104 (= E116), T105 (= T117), G106 (= G118), A107 (= A119), Q109 (= Q121), H110 (= H122), S185 (≠ T197), G228 (= G240), Y300 (= Y313)
5ixjD Tryptophan synthase beta-subunit from pyrococcus furiosus with l- threonine non-covalently bound in the active site (see paper)
63% identity, 96% coverage: 15:398/401 of query aligns to 3:384/394 of 5ixjD
5t6mA Structure of the tryptophan synthase b-subunit from pyroccus furiosus with b-methyltryptophan non-covalently bound (see paper)
63% identity, 95% coverage: 15:396/401 of query aligns to 3:382/383 of 5t6mA
7rnpA Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9_h275e with 4-cl-trp non-covalently bound (see paper)
63% identity, 95% coverage: 15:393/401 of query aligns to 3:381/384 of 7rnpA
6cutA Engineered holo trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3s)- isopropylserine bound as the external aldimine (see paper)
63% identity, 95% coverage: 15:393/401 of query aligns to 3:381/385 of 6cutA
- binding (2S,3S)-3-hydroxy-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]-4-methylpentanoic acid (non-preferred name): H81 (= H93), K82 (= K94), T105 (= T117), G106 (= G118), A107 (= A119), Q109 (= Q121), H110 (= H122), S185 (≠ T197), G227 (= G239), G229 (= G241), S230 (= S242), N231 (= N243), G298 (= G310), E345 (= E357), S371 (= S383)
6cuzA Engineered trpb from pyrococcus furiosus, pftrpb7e6 with (2s,3r)- ethylserine bound as the amino-acrylate (see paper)
63% identity, 95% coverage: 15:393/401 of query aligns to 3:381/383 of 6cuzA
- binding (2E)-2-[(E)-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)amino]pent-2-enoic acid: H81 (= H93), K82 (= K94), T105 (= T117), G106 (= G118), A107 (= A119), Q109 (= Q121), H110 (= H122), S185 (≠ T197), G227 (= G239), G229 (= G241), S230 (= S242), N231 (= N243), G298 (= G310), E345 (= E357), S371 (= S383)
5vm5D Engineered tryptophan synthase b-subunit from pyrococcus furiosus, pftrpb2b9, with ser bound (see paper)
63% identity, 95% coverage: 15:393/401 of query aligns to 3:379/383 of 5vm5D
- active site: K82 (= K94), E104 (= E116), S369 (= S383)
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H81 (= H93), K82 (= K94), T105 (= T117), G106 (= G118), A107 (= A119), Q109 (= Q121), H110 (= H122), S185 (≠ T197), G227 (= G239), G229 (= G241), S230 (= S242), N231 (= N243), G296 (= G310), E343 (= E357), S369 (= S383)
6usaB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk1-bound form (see paper)
60% identity, 98% coverage: 6:399/401 of query aligns to 6:402/404 of 6usaB
- active site: K97 (= K94), E119 (= E116), S386 (= S383)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H93), K97 (= K94), T120 (= T117), G121 (= G118), A122 (= A119), G123 (= G120), Q124 (= Q121), H125 (= H122), T200 (= T197), G242 (= G239), G244 (= G241), S245 (= S242), N246 (= N243), G313 (= G310), E360 (= E357), S386 (= S383)
- binding (3R,4R)-4-[4-(2-Chlorophenyl)piperazin-1-yl]-1,1-dioxothiolan-3-ol: F184 (≠ M181), W187 (= W184), Y196 (= Y193), F198 (≠ I195), G203 (= G200), P204 (= P201), F207 (≠ Y204), H290 (= H287), G291 (= G288)
6dweB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and brd0059-bound form
60% identity, 98% coverage: 6:399/401 of query aligns to 6:402/404 of 6dweB
- active site: K97 (= K94), E119 (= E116), S386 (= S383)
- binding (2R,3S,4R)-3-(2',6'-difluoro-4'-methyl[1,1'-biphenyl]-4-yl)-4-(fluoromethyl)azetidine-2-carbonitrile: F184 (≠ M181), Y196 (= Y193), F198 (≠ I195), P204 (= P201), F207 (≠ Y204), H290 (= H287)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H96 (= H93), K97 (= K94), T120 (= T117), G121 (= G118), A122 (= A119), G123 (= G120), Q124 (= Q121), H125 (= H122), T200 (= T197), G242 (= G239), G244 (= G241), S245 (= S242), N246 (= N243), G313 (= G310), E360 (= E357), S386 (= S383)
5tciH Crystal structure of tryptophan synthase from m. Tuberculosis - brd4592-bound form (see paper)
60% identity, 98% coverage: 6:399/401 of query aligns to 7:403/406 of 5tciH
- active site: K98 (= K94), E120 (= E116), S387 (= S383)
- binding (2R,3S,4R)-3-(2'-fluoro[1,1'-biphenyl]-4-yl)-4-(hydroxymethyl)azetidine-2-carbonitrile: P28 (= P24), L31 (= L27), Y197 (= Y193), F199 (≠ I195), P205 (= P201), F208 (≠ Y204), H291 (= H287)
6uapB Crystal structure of tryptophan synthase from m. Tuberculosis - open form with brd6309 bound
60% identity, 98% coverage: 6:399/401 of query aligns to 6:402/405 of 6uapB
- active site: K97 (= K94), E119 (= E116), S386 (= S383)
- binding (2R,3S,4R)-3-(4'-chloro-2',6'-difluoro[1,1'-biphenyl]-4-yl)-4-(fluoromethyl)azetidine-2-carbonitrile: I180 (≠ L177), N181 (= N178), F184 (≠ M181), Y196 (= Y193), F198 (≠ I195), P204 (= P201), F207 (≠ Y204), H290 (= H287)
6u6cB Crystal structure of tryptophan synthase from m. Tuberculosis - aminoacrylate- and gsk2-bound form (see paper)
60% identity, 98% coverage: 6:399/401 of query aligns to 7:403/405 of 6u6cB
- active site: K98 (= K94), E120 (= E116), S387 (= S383)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H97 (= H93), K98 (= K94), T121 (= T117), G122 (= G118), A123 (= A119), Q125 (= Q121), H126 (= H122), T201 (= T197), G243 (= G239), G245 (= G241), S246 (= S242), N247 (= N243), G314 (= G310), E361 (= E357), S387 (= S383)
- binding 1-(2-fluorobenzene-1-carbonyl)-N-methyl-2,3-dihydro-1H-indole-5-sulfonamide: Y26 (≠ F22), F185 (≠ M181), W188 (= W184), Y197 (= Y193), F199 (≠ I195), G204 (= G200), P205 (= P201), H291 (= H287), G292 (= G288)
5ocwB Structure of mycobacterium tuberculosis tryptophan synthase in space group f222 (see paper)
60% identity, 98% coverage: 6:399/401 of query aligns to 2:398/399 of 5ocwB
- active site: K93 (= K94), E115 (= E116), S382 (= S383)
- binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: H92 (= H93), K93 (= K94), T116 (= T117), G117 (= G118), A118 (= A119), Q120 (= Q121), H121 (= H122), T196 (= T197), G238 (= G239), G240 (= G241), S241 (= S242), N242 (= N243), G309 (= G310), E356 (= E357), S382 (= S383)
8vhhA Engineered holo tryptophan synthase (tm9d8 ) Derived from t. Maritima trpb (see paper)
61% identity, 95% coverage: 13:393/401 of query aligns to 2:377/389 of 8vhhA
- binding pyridoxal-5'-phosphate: H81 (= H93), K82 (= K94), Q109 (= Q121), S185 (≠ T197), G228 (= G240), G229 (= G241), S230 (= S242), N231 (= N243), G297 (= G310), E344 (= E357), S367 (= S383)
8eh0A Engineered tyrosine synthase (tmtyrs1) derived from t. Maritima trpb with ser bound as the amino-acrylate intermediate and complexed with quinoline n-oxide (see paper)
61% identity, 95% coverage: 13:393/401 of query aligns to 1:376/385 of 8eh0A
- binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: H80 (= H93), K81 (= K94), T104 (= T117), G105 (= G118), A106 (= A119), Q108 (= Q121), H109 (= H122), S184 (≠ T197), G228 (= G241), S229 (= S242), N230 (= N243), G296 (= G310), E343 (= E357), S366 (= S383), G367 (= G384)
- binding 1-oxo-1lambda~5~-quinoline: L160 (= L173), I164 (≠ L177), Y180 (= Y193), P182 (≠ I195), G183 (= G196), S184 (≠ T197), V186 (≠ A199), Y299 (= Y313)
Query Sequence
>WP_019555908.1 NCBI__GCF_000381085.1:WP_019555908.1
MTIDYSKYPDEHGHFGPYGGTFAPETLMAALEQLSQEYASVKNDPDFLKELAEDLQNYVG
RPTPLYYAKRWSESLGGAKIYLKREDLNHTGAHKVNNAIGQALLAKRLGKTRIIAETGAG
QHGVASATVAARLGLECVVYMGADDIVRQAPNVMRMKMLGATVIAVESGSRTLKDALNEA
MRDWVTNVDDTFYIIGTVAGPHPYPAMVRDFQAIIGRETKQQSLTQEGKLPDVLIACVGG
GSNAIGLFYEFLGDDSVKMVGVEPGGLGLETGKHAAPLCKGSPGVLHGNRTYLMQDENGQ
ILGTHSISAGLDYPGVGPEHAWLKDIKRVEYVSIDDEEALQGFRDLTKYEGIIPALESSH
AIAYATKLAPTLSKDQVIVVNLSGRGDKDMNTIAQIEGFEV
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory