SitesBLAST
Comparing WP_019556125.1 NCBI__GCF_000381085.1:WP_019556125.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8y1jA Structure of the pyridoxal 5'-phosphate-dependent (plp) threonine deaminase ilva1 from pseudomonas aeruginosa pao1 (see paper)
58% identity, 99% coverage: 3:504/508 of query aligns to 2:502/502 of 8y1jA
- binding 2-ketobutyric acid: K50 (= K51), S74 (= S75), H78 (= H79), E336 (= E337), R337 (≠ T338), P338 (= P339), S340 (= S341), F341 (= F342), Y358 (= Y360), N448 (= N450), I449 (= I451)
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
53% identity, 99% coverage: 4:504/508 of query aligns to 15:512/514 of P04968
- K62 (= K51) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N78) binding pyridoxal 5'-phosphate
- GGGGL 188:192 (= GGGGL 178:182) binding pyridoxal 5'-phosphate
- S315 (= S305) binding pyridoxal 5'-phosphate
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
53% identity, 99% coverage: 4:504/508 of query aligns to 11:492/494 of 1tdjA
- active site: K58 (= K51), A83 (= A76), E209 (= E203), S213 (≠ A207), C215 (≠ S209), G237 (= G231), L310 (≠ V304), S311 (= S305)
- binding pyridoxal-5'-phosphate: F57 (= F50), K58 (= K51), N85 (= N78), G184 (= G178), G185 (= G179), G186 (= G180), G187 (= G181), G237 (= G231), E282 (= E276), S311 (= S305), G312 (= G306)
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
36% identity, 62% coverage: 3:318/508 of query aligns to 10:322/326 of 2gn2A
- active site: K56 (= K51), A81 (= A76), Q207 (≠ E203), V211 (≠ A207), G213 (≠ S209), G235 (= G231), I308 (≠ V304), S309 (= S305)
- binding cytidine-5'-monophosphate: R51 (≠ P46), T52 (≠ V47), G53 (≠ F48), A114 (= A109), D117 (≠ R112), Y118 (≠ L113), N312 (= N308)
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
34% identity, 60% coverage: 10:316/508 of query aligns to 1:315/319 of 2zr8A
- active site: K53 (= K51), S78 (≠ A76), E204 (= E203), G208 (≠ A207), D210 (≠ S209), G232 (= G231), I303 (≠ V304), S304 (= S305)
- binding magnesium ion: E204 (= E203), G208 (≠ A207), D210 (≠ S209)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F50), K53 (= K51), S77 (= S75), S78 (≠ A76), N80 (= N78), H81 (= H79), P147 (= P145), G179 (= G178), G180 (= G179), G181 (= G180), G182 (= G181), G232 (= G231), E277 (= E276), T279 (≠ A278), S304 (= S305)
- binding serine: S78 (≠ A76), R129 (≠ A127), D231 (= D230), G232 (= G231), A233 (≠ V232), Q234 (≠ A233), T235 (≠ V234)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
34% identity, 60% coverage: 10:316/508 of query aligns to 1:315/319 of 2zpuA
- active site: K53 (= K51), S78 (≠ A76), E204 (= E203), G208 (≠ A207), D210 (≠ S209), G232 (= G231), I303 (≠ V304), S304 (= S305)
- binding magnesium ion: E204 (= E203), G208 (≠ A207), D210 (≠ S209)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F50), K53 (= K51), S77 (= S75), S78 (≠ A76), N80 (= N78), H81 (= H79), P147 (= P145), G179 (= G178), G180 (= G179), G181 (= G180), G182 (= G181), G232 (= G231), E277 (= E276), T279 (≠ A278), S304 (= S305)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see 2 papers)
34% identity, 60% coverage: 10:316/508 of query aligns to 5:319/323 of O59791
- K57 (= K51) active site, Proton acceptor; modified: Lysino-D-alanine (Lys); alternate; modified: N6-(pyridoxal phosphate)lysine; alternate
- S82 (≠ A76) active site, Proton acceptor; mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
- N84 (= N78) binding pyridoxal 5'-phosphate
- G183 (= G178) binding pyridoxal 5'-phosphate
- G184 (= G179) binding pyridoxal 5'-phosphate
- G185 (= G180) binding pyridoxal 5'-phosphate
- G186 (= G181) binding pyridoxal 5'-phosphate
- L187 (= L182) binding pyridoxal 5'-phosphate
- E208 (= E203) binding Mg(2+)
- G212 (≠ A207) binding Mg(2+)
- D214 (≠ S209) binding Mg(2+)
- S308 (= S305) binding pyridoxal 5'-phosphate
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
34% identity, 59% coverage: 18:316/508 of query aligns to 19:314/318 of 1wtcA
- active site: K52 (= K51), S77 (≠ A76), E203 (= E203), G207 (≠ A207), D209 (≠ S209), G231 (= G231), I302 (≠ V304), S303 (= S305)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ S19), K47 (≠ P46), M48 (≠ V47), A109 (≠ N108), A110 (= A109), Y114 (≠ L113)
- binding magnesium ion: E203 (= E203), G207 (≠ A207), D209 (≠ S209)
- binding pyridoxal-5'-phosphate: F51 (= F50), K52 (= K51), N79 (= N78), G178 (= G178), G179 (= G179), G180 (= G180), G181 (= G181), G231 (= G231), E276 (= E276), T278 (≠ A278), S303 (= S305)
1v71A Crystal structure of s.Pombe serine racemase
34% identity, 59% coverage: 18:316/508 of query aligns to 19:314/318 of 1v71A
- active site: K52 (= K51), S77 (≠ A76), E203 (= E203), G207 (≠ A207), D209 (≠ S209), G231 (= G231), I302 (≠ V304), S303 (= S305)
- binding magnesium ion: E203 (= E203), G207 (≠ A207), D209 (≠ S209)
- binding pyridoxal-5'-phosphate: F51 (= F50), K52 (= K51), N79 (= N78), G178 (= G178), G179 (= G179), G180 (= G180), G181 (= G181), G231 (= G231), E276 (= E276), T278 (≠ A278), S303 (= S305), G304 (= G306)
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
35% identity, 55% coverage: 36:314/508 of query aligns to 53:332/339 of Q7XSN8
- E219 (= E203) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (≠ S209) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
34% identity, 55% coverage: 36:313/508 of query aligns to 38:312/319 of A4F2N8
- K53 (= K51) mutation to A: Loss of enzymatic activity.
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
34% identity, 57% coverage: 22:311/508 of query aligns to 19:316/320 of 7nbhAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E203), A211 (= A207), D213 (≠ S209), G236 (= G231), L309 (≠ V304), S310 (= S305)
- binding calcium ion: E207 (= E203), A211 (= A207), D213 (≠ S209)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A76), G85 (≠ A80), Q86 (= Q81), K111 (= K106), I115 (≠ V110), Y118 (≠ L113), D235 (= D230), P281 (= P277), N313 (= N308), V314 (≠ M309), D315 (≠ N310)
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
34% identity, 57% coverage: 22:311/508 of query aligns to 19:316/322 of 7nbgAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E203), A211 (= A207), D213 (≠ S209), G236 (= G231), L309 (≠ V304), S310 (= S305)
- binding calcium ion: E207 (= E203), A211 (= A207), D213 (≠ S209)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (= V232), T282 (≠ A278), S310 (= S305), G311 (= G306)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A76), G85 (≠ A80), Q86 (= Q81), I101 (= I96), K111 (= K106), I115 (≠ V110), Y118 (≠ L113)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
34% identity, 57% coverage: 22:311/508 of query aligns to 19:316/323 of 7nbfAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E203), A211 (= A207), D213 (≠ S209), G236 (= G231), L309 (≠ V304), S310 (= S305)
- binding calcium ion: E207 (= E203), A211 (= A207), D213 (≠ S209)
- binding magnesium ion: N244 (≠ I240)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (= V232), T282 (≠ A278), S310 (= S305), G311 (= G306)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: H21 (≠ E24), L22 (= L25), T23 (≠ A26), P24 (= P27), L26 (= L29), T27 (≠ S30), F46 (≠ L44)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
34% identity, 57% coverage: 22:311/508 of query aligns to 19:316/323 of 7nbdAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E203), A211 (= A207), D213 (≠ S209), G236 (= G231), L309 (≠ V304), S310 (= S305)
- binding calcium ion: E207 (= E203), A211 (= A207), D213 (≠ S209)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F268), L278 (≠ I274), V314 (≠ M309), L316 (≠ F311)
- binding magnesium ion: N244 (≠ I240)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (= V232), E280 (= E276), T282 (≠ A278), S310 (= S305), G311 (= G306)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
34% identity, 57% coverage: 22:311/508 of query aligns to 19:316/323 of 7nbcCCC
- active site: K53 (= K51), S81 (≠ A76), E207 (= E203), A211 (= A207), D213 (≠ S209), G236 (= G231), L309 (≠ V304), S310 (= S305)
- binding biphenyl-4-ylacetic acid: T78 (= T73), H79 (≠ A74), H84 (= H79), V148 (≠ I143), H149 (= H144), P150 (= P145)
- binding calcium ion: E207 (= E203), A211 (= A207), D213 (≠ S209)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (= V232), T282 (≠ A278), S310 (= S305), G311 (= G306)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
34% identity, 57% coverage: 22:311/508 of query aligns to 19:316/323 of 7nbcAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E203), A211 (= A207), D213 (≠ S209), G236 (= G231), L309 (≠ V304), S310 (= S305)
- binding calcium ion: E207 (= E203), A211 (= A207), D213 (≠ S209)
- binding magnesium ion: N244 (≠ I240)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G178), G183 (= G179), G184 (= G180), G185 (= G181), M186 (≠ L182), G236 (= G231), V237 (= V232), T282 (≠ A278), S310 (= S305), G311 (= G306)
Sites not aligning to the query:
Q9GZT4 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Homo sapiens (Human) (see 4 papers)
34% identity, 57% coverage: 22:311/508 of query aligns to 22:319/340 of Q9GZT4
- S31 (≠ K31) binding ATP
- S32 (≠ R32) binding ATP
- I33 (≠ L33) binding ATP
- K51 (≠ P46) binding ATP
- T52 (≠ V47) binding ATP
- K56 (= K51) modified: N6-(pyridoxal phosphate)lysine
- P69 (≠ E64) binding Ca(2+)
- T81 (= T73) binding Ca(2+)
- N86 (= N78) binding pyridoxal 5'-phosphate
- Q89 (= Q81) binding ATP
- Y121 (≠ L113) binding ATP
- D178 (= D171) binding Mg(2+)
- G185 (= G178) binding pyridoxal 5'-phosphate
- G186 (= G179) binding pyridoxal 5'-phosphate
- G187 (= G180) binding pyridoxal 5'-phosphate
- G188 (= G181) binding pyridoxal 5'-phosphate
- M189 (≠ L182) binding pyridoxal 5'-phosphate
- E210 (= E203) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- A214 (= A207) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- D216 (≠ S209) binding Ca(2+); binding Mg(2+); binding Mn(2+)
- N247 (≠ I240) binding Ca(2+); binding Mg(2+)
- K279 (≠ R272) binding ATP
- S313 (= S305) binding pyridoxal 5'-phosphate
- N316 (= N308) binding ATP
Sites not aligning to the query:
6zspAAA serine racemase bound to atp and malonate. (see paper)
33% identity, 57% coverage: 22:311/508 of query aligns to 19:309/320 of 6zspAAA
- active site: K53 (= K51), S74 (≠ A76), E200 (= E203), A204 (= A207), D206 (≠ S209), G229 (= G231), L302 (≠ V304), S303 (= S305)
- binding adenosine-5'-triphosphate: S28 (≠ K31), S29 (≠ R32), I30 (≠ L33), K48 (≠ P46), T49 (≠ V47), Q79 (= Q81), Y111 (≠ L113), E266 (= E269), R267 (≠ D270), K269 (≠ R272), N306 (= N308)
- binding magnesium ion: E200 (= E203), A204 (= A207), D206 (≠ S209)
- binding malonate ion: K53 (= K51), S73 (= S75), S74 (≠ A76), N76 (= N78), H77 (= H79), R125 (≠ A127), G229 (= G231), S232 (≠ A235)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
33% identity, 57% coverage: 22:311/508 of query aligns to 20:312/322 of 3l6bA
- active site: K54 (= K51), S77 (≠ A76), E203 (= E203), A207 (= A207), D209 (≠ S209), G232 (= G231), T278 (≠ A278), L305 (≠ V304), S306 (= S305)
- binding malonate ion: K54 (= K51), S76 (= S75), S77 (≠ A76), N79 (= N78), H80 (= H79), R128 (≠ A127), G232 (= G231)
- binding manganese (ii) ion: E203 (= E203), A207 (= A207), D209 (≠ S209)
- binding pyridoxal-5'-phosphate: F53 (= F50), K54 (= K51), N79 (= N78), G178 (= G178), G179 (= G179), G180 (= G180), G181 (= G181), M182 (≠ L182), V233 (= V232), E276 (= E276), T278 (≠ A278), S306 (= S305), G307 (= G306)
Query Sequence
>WP_019556125.1 NCBI__GCF_000381085.1:WP_019556125.1
MPEEILRRVLTAPVYDVASETALELAPLLSKRLQNQVWLKREDLQPVFSFKIRGAYNRMV
HLSESERKAGVITASAGNHAQGVALSANKMGIDATIVMPRTTPPIKVNAVRRLGGNVVLE
GDSFDEASRFAKALEVEKKLTYIHPYDDLDVIAGQGTIALEILRQHSKPFDVIFIPVGGG
GLISGIASVIKQVSPSTRIIGIEPEDAASMTEALKAGERIVLDQVGIFADGVAVAQVGEI
PFRIAQTCVDEMFTVTTDEICAAVKDIFEDTRAIAEPAGALSVAGMKKFVDKYAVSGLDM
ACVVSGANMNFDRLRHVSERTEIGEKREALFAVTIRETPGSFKKFCELLGNRRSITEFNY
RYSDSKQAVVFVGVRTEGGHAEKQAILNSLHQHTYPVQDMSDNEMAKLHLRYMVGGHAKG
VNNELLYRFEFPERPGALLKFLINMSEEWNISLFHYRNHGAAYGRVLIGVQVPPEQQADF
EIYLKSLGYPFVNEQENPGYQLFLRPTH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory