SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_019556825.1 NCBI__GCF_000381085.1:WP_019556825.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5mr0D Thermophilic archaeal branched-chain amino acid transaminases from geoglobus acetivorans and archaeoglobus fulgidus: biochemical and structural characterisation (see paper)
35% identity, 91% coverage: 11:278/295 of query aligns to 4:273/290 of 5mr0D

query
sites
5mr0D

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active site: F32 (≠ Y39), G34 (≠ V41), K150 (= K154), E183 (= E187), L206 (= L210)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: R51 (= R58), G100 (≠ Q106), L101 (≠ R107), K150 (= K154), Y154 (≠ L158), E183 (= E187), G186 (≠ S190), D187 (≠ S191), L206 (= L210), I209 (≠ V214), T210 (= T215), G245 (≠ S250), T246 (≠ S251)

5e25A Crystal structure of branched-chain aminotransferase from thermophilic archaea geoglobus acetivorans complexed with alpha-ketoglutarate (see paper)
36% identity, 95% coverage: 7:285/295 of query aligns to 1:281/290 of 5e25A

query
sites
5e25A

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active site: F33 (≠ Y39), G35 (≠ V41), K151 (= K154), E184 (= E187), L207 (= L211)
binding 2-oxoglutaric acid: Y88 (= Y94), K151 (= K154), T247 (≠ S251), A248 (≠ T252)
binding pyridoxal-5'-phosphate: R52 (= R58), K151 (= K154), Y155 (≠ L158), E184 (= E187), G187 (≠ S190), D188 (≠ S191), L207 (= L211), G209 (= G213), I210 (≠ V214), T211 (= T215), G246 (≠ S250), T247 (≠ S251)

3daaA Crystallographic structure of d-amino acid aminotransferase inactivated by pyridoxyl-d-alanine (see paper)
36% identity, 92% coverage: 14:283/295 of query aligns to 6:273/277 of 3daaA

query
sites
3daaA

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active site: Y31 (= Y39), V33 (= V41), K145 (= K154), E177 (= E187), L201 (= L211)
binding n-(5'-phosphopyridoxyl)-d-alanine: Y31 (= Y39), R50 (= R58), K145 (= K154), E177 (= E187), S180 (= S190), S181 (= S191), L201 (= L211), G203 (= G213), I204 (≠ V214), T205 (= T215), S240 (= S250), T241 (≠ S251)

2daaA Crystallographic structure of d-amino acid aminotransferase inactivated by d-cycloserine
36% identity, 92% coverage: 14:283/295 of query aligns to 6:273/277 of 2daaA

query
sites
2daaA

N

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active site: Y31 (= Y39), V33 (= V41), K145 (= K154), E177 (= E187), L201 (= L211)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Y31 (= Y39), V33 (= V41), R50 (= R58), R98 (≠ Q106), H100 (≠ D108), K145 (= K154), E177 (= E187), S180 (= S190), S181 (= S191), N182 (= N192), L201 (= L211), G203 (= G213), I204 (≠ V214), T205 (= T215), T241 (≠ S251)

1daaA Crystallographic structure of d-amino acid aminotransferase complexed with pyridoxal-5'-phosphate (see paper)
36% identity, 92% coverage: 14:283/295 of query aligns to 6:273/277 of 1daaA

query
sites
1daaA

N

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I

Q

T

N

I

P

P

L

Y

T

T

D

K

A

D

E

K

W

F

L

H

E

Q

L

L

S

H

T

E

L

L

I

V

E

E

K

K

H

N

P

E

W

L

S

N

D

T

Q

G

Y

H

I

I

Y

Y

L

F

Q

Q

I

V

T

T

R

R

G

G

I

T

Q

S

I

P

Q

R

R

A

D

H

H

Q

L

F

P

P

E

E

D

N

G

T

L

V

I

K

P

P

T

V

I

I

Y

I

A

G

Y

Y

S

T

N

K

-

E

-

N

-

P

-

R

P

P

L

L

K

E

A

N

V

-

S

-

D

-

I

-

I

L

E

E

N

K

G

G

I

V

S

K

A

A

I

T

T

F

L

V

E

E

D

D

I

I

R

R

W

W

L

L

N

R

C

C

D

D

I

I

K
|
K

A

S

I

L

T

N

L

L

P

G

N

A

I

V

M

L

M

A

K

K

L

Q

T

E

A

A

K

H

A

E

R

K

H

G

A

C

D

Y

D

E

A

A

I

I

L

L

V

-

S

H

R

R

E

N

G

N

F

T

I

V

T

T

E
|
E

G

G

T

S

S
|
S

N

N

C

V

F

F

I

G

V

I

K

K

N

D

G

G

T

I

L

L

I

Y

T

T

P

H

P

P

N

A

G

N

H

N

S

M

L

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

R

R

M

D

V

V

I

V

E

I

R

A

V

C

A

A

I

N

A

E

H

I

N

N

I

M

P

P

M

V

L

K

E

E

Q

I

Q

P

I

F

T

T

L

T

T

H

D

E

L

A

E

L

S

K

A

M

D

D

E

E

I

L

W

F

L

V

S

T

S
|
S

S
x
T

T

T

K

S

D

E

A

I

L

T

P

P

V

V

T

I

R

E

L

I

N

D

D

G

S

K

A

L

V

I

G

R

T

D

G

G

K

K

P

V

G

G

A

E

V

W

W

T

Q

R

K

K

M

L

R

Q

G

K

F

Q

Y

F

Q

E

active site: Y31 (= Y39), V33 (= V41), K145 (= K154), E177 (= E187), L201 (= L211)
binding pyridoxal-5'-phosphate: R50 (= R58), K145 (= K154), E177 (= E187), S180 (= S190), S181 (= S191), L201 (= L211), G203 (= G213), I204 (≠ V214), T205 (= T215), S240 (= S250), T241 (≠ S251)

3lqsA Complex structure of d-amino acid aminotransferase and 4-amino-4,5- dihydro-thiophenecarboxylic acid (adta) (see paper)
36% identity, 92% coverage: 14:283/295 of query aligns to 6:273/280 of 3lqsA

query
sites
3lqsA

N

N

G

D

Q

Q

Y

I

M

V

P

K

M

D

L

E

E

E

S

V

K

K

I

I

S

D

T

K

Q

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

V

V

Y
|
Y

E

E

V
|
V

I

V

P

K

V

V

Y

Y

D

N

R

G

K

E

L

M

F

F

A

T

F

V

P

N

E

E

H

H

L

I

Q

D

R
|
R

L

L

K

Y

N

A

S

S

L

A

H

E

A

K

T

I

S

R

I

I

Q

T

N

I

P

P

L

Y

T

T

D

K

A

D

E

K

W

F

L

H

E

Q

L

L

S

H

T

E

L

L

I

V

E

E

K

K

H

N

P

E

W

L

S

N

D

T

Q

G

Y

H

I

I

Y

Y

L

F

Q

Q

I

V

T

T

R

R

G

G

I

T

Q

S

I

P

Q

R

R

A

D

H

H

Q

L

F

P

P

E

E

D

N

G

T

L

V

I

K

P

P

T

V

I

I

Y

I

A

G

Y

Y

S

T

N

K

-

E

-

N

-

P

-

R

P

P

L

L

K

E

A

N

V

-

S

-

D

-

I

-

I

L

E

E

N

K

G

G

I

V

S

K

A

A

I

T

T

F

L

V

E

E

D

D

I

I

R

R

W

W

L

L

N

R

C

C

D

D

I

I

K
|
K

A

S

I

L

T

N

L

L

P

G

N

A

I

V

M

L

M

A

K

K

L

Q

T

E

A

A

K

H

A

E

R

K

H

G

A

C

D

Y

D

E

A

A

I

I

L

L

V

-

S

H

R

R

E

N

G

N

F

T

I

V

T

T

E
|
E

G

G

T

S

S
|
S

N
|
N

C

V

F

F

I

G

V

I

K

K

N

D

G

G

T

I

L

L

I

Y

T

T

P

H

P

P

N

A

G

N

H

N

S

M

L

I

L
|
L

P

K

G
|
G

V
x
I

T
|
T

R

R

M

D

V

V

I

V

E

I

R

A

V

C

A

A

I

N

A

E

H

I

N

N

I

M

P

P

M

V

L

K

E

E

Q

I

Q

P

I

F

T

T

L

T

T

H

D

E

L

A

E

L

S

K

A

M

D

D

E

E

I

L

W

F

L

V

S

T

S
|
S

S
x
T

T
|
T

K

S

D

E

A

I

L

T

P

P

V

V

T

I

R

E

L

I

N

D

D

G

S

K

A

L

V

I

G

R

T

D

G

G

K

K

P

V

G

G

A

E

V

W

W

T

Q

R

K

K

M

L

R

Q

G

K

F

Q

Y

F

Q

E

active site: Y31 (= Y39), V33 (= V41), K145 (= K154), E177 (= E187), L201 (= L211)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]thiophene-2-carboxylic acid: V33 (= V41), R50 (= R58), E177 (= E187), S180 (= S190), S181 (= S191), N182 (= N192), L201 (= L211), G203 (= G213), I204 (≠ V214), T205 (= T215), S240 (= S250), T241 (≠ S251), T242 (= T252)

P19938 D-alanine aminotransferase; D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase; EC 2.6.1.21 from Bacillus sp. (strain YM-1) (see 5 papers)
36% identity, 92% coverage: 14:283/295 of query aligns to 7:274/283 of P19938

query
sites
P19938

N

N

G

D

Q

Q

Y

I

M

V

P

K

M

D

L

E

E

E

S

V

K

K

I

I

S

D

T

K

Q

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

V

V

Y
|
Y

E

E

V

V

I

V

P

K

V

V

Y

Y

D

N

R

G

K

E

L

M

F

F

A

T

F

V

P

N

E

E

H

H

L

I

Q

D

R
|
R

L

L

K

Y

N

A

S

S

L

A

H

E

A

K

T

I

S

R

I

I

Q

T

N

I

P

P

L

Y

T

T

D

K

A

D

E

K

W

F

L

H

E

Q

L

L

S

H

T

E

L

L

I

V

E

E

K

K

H

N

P

E

W

L

S

N

D

T

Q

G

Y

H

I

I

Y

Y

L

F

Q

Q

I

V

T

T

R

R

G

G

I

T

Q

S

I

P

Q
x
R

R

A

D
x
H

H

Q

L

F

P

P

E

E

D

N

G

T

L

V

I

K

P

P

T

V

I

I

Y

I

A

G

Y

Y

S

T

N

K

-

E

-

N

-

P

-

R

P

P

L

L

K

E

A

N

V

-

S

-

D

-

I

-

I

L

E

E

N

K

G

G

I

V

S

K

A

A

I

T

T

F

L

V

E

E

D

D

I

I

R

R

W

W

L

L

N

R

C

C

D

D

I

I

K
|
K

A

S

I

L

T

N

L

L

P

G

N

A

I

V

M

L

M

A

K

K

L

Q

T

E

A

A

K

H

A

E

R

K

H

G

A

C

D

Y

D

E

A

A

I

I

L

L

V

-

S

H

R

R

E

N

G

N

F

T

I

V

T

T

E
|
E

G

G

T

S

S

S

N

N

C

V

F

F

I

G

V

I

K

K

N

D

G

G

T

I

L

L

I

Y

T

T

P

H

P

P

N

A

G

N

H

N

S

M

L

I

L
|
L

P

K

G

G

V

I

T

T

R

R

M

D

V

V

I

V

E

I

R

A

V

C

A

A

I

N

A

E

H

I

N

N

I

M

P

P

M

V

L

K

E

E

Q

I

Q

P

I

F

T

T

L

T

T

H

D

E

L

A

E

L

S

K

A

M

D

D

E

E

I

L

W

F

L

V

S

T

S

S

S

T

T

T

K

S

D

E

A

I

L

T

P

P

V

V

T

I

R

E

L

I

N

D

D

G

S

K

A

L

V

I

G

R

T

D

G

G

K

K

P

V

G

G

A

E

V

W

W

T

Q

R

K

K

M

L

R

Q

G

K

F

Q

Y

F

Q

E

Y32 (= Y39) binding substrate
R51 (= R58) binding pyridoxal 5'-phosphate
R99 (≠ Q106) binding substrate
H101 (≠ D108) binding substrate
K146 (= K154) active site, Proton acceptor; modified: N6-(pyridoxal phosphate)lysine
E178 (= E187) binding pyridoxal 5'-phosphate; mutation to K: Loss of transaminase activity and small gain in racemase activity.
L202 (= L211) mutation to A: Inactivates enzyme.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1a0gB L201a mutant of d-amino acid aminotransferase complexed with pyridoxamine-5'-phosphate (see paper)
35% identity, 92% coverage: 14:283/295 of query aligns to 6:273/282 of 1a0gB

query
sites
1a0gB

N

N

G

D

Q

Q

Y

I

M

V

P

K

M

D

L

E

E

E

S

V

K

K

I

I

S

D

T

K

Q

E

D

D

R

R

G

G

F

Y

L

Q

F

F

G

G

D

D

G

G

V

V

Y
|
Y

E

E

V
|
V

I

V

P

K

V

V

Y

Y

D

N

R

G

K

E

L

M

F

F

A

T

F

V

P

N

E

E

H

H

L

I

Q

D

R
|
R

L

L

K

Y

N

A

S

S

L

A

H

E

A

K

T

I

S

R

I

I

Q

T

N

I

P

P

L

Y

T

T

D

K

A

D

E

K

W

F

L

H

E

Q

L

L

S

H

T

E

L

L

I

V

E

E

K

K

H

N

P

E

W

L

S

N

D

T

Q

G

Y

H

I

I

Y

Y

L

F

Q

Q

I

V

T

T

R

R

G

G

I

T

Q

S

I

P

Q

R

R

A

D

H

H

Q

L

F

P

P

E

E

D

N

G

T

L

V

I

K

P

P

T

V

I

I

Y

I

A

G

Y

Y

S

T

N

K

-

E

-

N

-

P

-

R

P

P

L

L

K

E

A

N

V

-

S

-

D

-

I

-

I

L

E

E

N

K

G

G

I

V

S

K

A

A

I

T

T

F

L

V

E

E

D

D

I

I

R

R

W

W

L

L

N

R

C

C

D

D

I

I

K
|
K

A

S

I

L

T

N

L

L

P

G

N

A

I

V

M

L

M

A

K

K

L

Q

T

E

A

A

K

H

A

E

R

K

H

G

A

C

D

Y

D

E

A

A

I

I

L

L

V

-

S

H

R

R

E

N

G

N

F

T

I

V

T

T

E
|
E

G

G

T

S

S
|
S

N

N

C

V

F

F

I

G

V

I

K

K

N

D

G

G

T

I

L

L

I

Y

T

T

P

H

P

P

N

A

G

N

H

N

S

M

L

I

L
x
A

P

K

G
|
G

V
x
I

T
|
T

R

R

M

D

V

V

I

V

E

I

R

A

V

C

A

A

I

N

A

E

H

I

N

N

I

M

P

P

M

V

L

K

E

E

Q

I

Q

P

I

F

T

T

L

T

T

H

D

E

L

A

E

L

S

K

A

M

D

D

E

E

I

L

W

F

L

V

S

T

S
|
S

S
x
T

T

T

K

S

D

E

A

I

L

T

P

P

V

V

T

I

R

E

L

I

N

D

D

G

S

K

A

L

V

I

G

R

T

D

G

G

K

K

P

V

G

G

A

E

V

W

W

T

Q

R

K

K

M

L

R

Q

G

K

F

Q

Y

F

Q

E

active site: Y31 (= Y39), V33 (= V41), K145 (= K154), E177 (= E187), A201 (≠ L211)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R50 (= R58), K145 (= K154), E177 (= E187), S180 (= S190), S181 (= S191), G203 (= G213), I204 (≠ V214), T205 (= T215), S240 (= S250), T241 (≠ S251)

7p3tB Transaminase of gamma-proteobacterium (see paper)
30% identity, 93% coverage: 8:282/295 of query aligns to 3:279/299 of 7p3tB

query
sites
7p3tB

E

E

Q

P

I

I

V

I

Y

Y

L

I

N

N

G

G

Q

D

Y

Y

M

L

P

P

M

L

L

S

E

Q

S

A

K

R

I

V

S

S

T

P

Q

V

D

D

R

Q

G

G

F

F

L

L

F

L

G

G

D

D

G

G

V

V

Y

F

E

D

V

V

I

V

P

S

V

A

Y

W

D

K

R

G

K

N

L

I

F

F

A

K

F

L

P

D

E

A

H

H

L

L

Q

D

R
|
R

L

F

K

F

N

D

S

S

L

I

H

Q

A

A

T

A

S

R

I

L

Q

N

N

H

P

D

L

M

T

S

D

R

A

D

E

A

W

W

L

K

E

E

L

A

L

I

S

I

T

E

L

T

I

T

E

R

K

R

H

N

P

G

W

L

S

D

D

D

Q

A

Y

S

I

I

Y

R

L

F

Q

I

I

V

T

T

R

R

G

G

I

E

Q

P

I

K

Q

G

R

V

D

V

H

A

L

D

P

P

E

R

D

D

G

-

L

F

I

K

P

P

T

T

I

C

Y

I

A

V

Y

W

S

V

N

A

P

P

L

Y

K

I

A

F

V

L

S

A

D

D

-

E

D

E

I

K

I

R

E

R

N

N

G

G

I

I

S

R

A

-

I

L

T

M

L

I

E

S

D

A

I

T

R

R

W

G

L

F

N

P

C

A

D

D

I

T

-

L

-

D

-

P

-

R

-

Y

K
|
K

A

C

I

L

T

D

L
x
R

L

L

P

H

N

S

I

Q

M

L

M

I

K

R

L

L

T

E

A

A

K

L

A

E

R

A

H

G

A

Y

D

D

A

A

I

L

L

W

V

L

S

D

R

H

E

S

G

G

F

H

I

V

T

S

E
|
E

G
x
S

T
x
A

S
x
A

S
|
S

N

N

C

L

F

F

I

I

V

V

K

K

N

N

G

G

T

V

L

L

I

Y

T

T

P

P

P

S

N

A

G

G

H

-

S

-

L

I

L

L

P

R

G
|
G

V
x
I

T
|
T

R

R

M

D

V

T

I

I

E

L

R

E

V

L

A

A

I

T

A

E

H

L

N

D

I

I

P

P

M

W

L

K

E

E

Q

R

Q

Q

I

L

T

S

L

A

T

F

D

D

L

V

E

Y

S

I

A

A

D

D

E

E

I

V

W

F

L

T

S

C

S

S

S
x
T

T

A

K

G

D

G

A

A

L

L

P

P

V

V

T

R

R

E

L

V

N

A

D

G

S

R

A

T

V

I

G

R

T

G

G

T

K

T

P

P

G

G

A

P

V

I

W

T

Q

Q

K

A

M

I

R

D

G

N

F

A

Y

Y

binding pyridoxal-5'-phosphate: R53 (= R58), K153 (= K154), R157 (≠ L158), E186 (= E187), S187 (≠ G188), A188 (≠ T189), A189 (≠ S190), S190 (= S191), G210 (= G213), I211 (≠ V214), T212 (= T215), T248 (≠ S251)

6snlD (R)-selective amine transaminase from exophiala sideris (see paper)
28% identity, 96% coverage: 2:285/295 of query aligns to 20:308/320 of 6snlD

query
sites
6snlD

N

S

T

S

N

N

P

P

I

L

T

S

E

K

Q

G

I

I

V

A

Y

W

L

V

N

F

G

G

Q

E

Y

L

M

V

P

P

M

L

L

A

E

E

S

A

K

R

I

I

S

P

T

L

Q

M

D

D

R

Q

G

G

F

F

L

M

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V

V

I

P

P

S

V

V

Y

W

D

D

R

G

K

R

L

F

F

F

A

R

F

L

P

D

E

D

H

H

L

I

Q

A

R
|
R

L

L

K

E

N

A

S

S

L

C

H

A

A

K

T

M

S

R

I

L

Q

Q

N

L

P

P

L

L

T

P

D

R

A

E

E

E

W

V

L

K

E

Q

L

I

L

L

S

V

T

D

L

M

I

V

E

A

K

K

H

S

P

E

W

I

S

K

D

D

Q

A

Y

F

I

V

Y

E

L

L

Q

I

I

V

T

T

R

R

G

G

I

L

Q

K

I

G

Q

V

R

R

D

G

H

H

L

T

P

P

E

G

D

E

G

T

L

F

I

K

P

N

T

H

I

L

Y

Y

A

M

Y

F

S

V

N

Q

P

P

L

Y

K

V

A

W

V

V

S

M

D

D

-

P

D

D

I

I

I

Q

E

K

N

T

G

G

I

G

S

K

A

A

I

I

T

I

L

A

E

R

D

T

I

V

R

R

W

R

-

I

-

P

-

G

-

S

L

I

N

D

C

P

D

T

I

V

K
|
K

A

N

I

L

T

Q

L

W

L

G

P

D

N

L

I

T

M

R

M

G

K

L

L

F

T

E

A

A

K

A

A

D

R

R

H

G

A

A

D

T

D

Y

A

P

I

F

L

L

V

T

S

D

R

G

E

D

G

A

F

N

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S
x
F

N

N

C

V

F

L

I

L

V

V

K

K

N

D

G

G

T

V

L

I

I

Y

T

T

P

P

P

D

N

R

G

G

H

-

S

-

L

V

L
|
L

P

Q

G
|
G

V
|
V

T
|
T

R

R

M

K

V

S

I

C

E

I

R

D

V

V

A

A

I

K

A

S

H

L

N

G

I

I

P

E

M

V

L

R

E

V

Q

Q

Q

F

I

V

T

P

L

I

T

Q

D

M

L

A

E

Y

S

D

A

A

D

D

E

E

I

I

W

F

L

M

S

A

S

T

S
x
T

T

A

K

G

D

G

A

I

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

D

S

K

A

P

V

I

G

Q

T

S

G

G

K

K

P

V

G

G

A

P

V

I

W

T

Q

K

K

K

M

I

-

W

R

D

G

G

F

Y

Y

W

Q

A

E

I

H

H

active site: Y57 (= Y39), K178 (= K154), E211 (= E187), L233 (= L211)
binding pyridoxal-5'-phosphate: R76 (= R58), K178 (= K154), E211 (= E187), G214 (≠ S190), F215 (≠ S191), L233 (= L211), G235 (= G213), V236 (= V214), T237 (= T215), T273 (≠ S251)

4cmfA The (r)-selective transaminase from nectria haematococca with inhibitor bound (see paper)
27% identity, 98% coverage: 2:289/295 of query aligns to 21:316/322 of 4cmfA

query
sites
4cmfA

N

S

T

K

N

N

P

P

I

L

T

A

E

K

Q

G

I

V

V

A

Y

W

L

I

N

Q

G

G

Q

E

Y

L

M

V

P

P

M

L

L

H

E

E

S

A

K

R

I

I

S

P

T

L

Q

L

D

D

R

Q

G

G

F

F

L

M

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V
|
V

I

P

P

S

V

V

Y

W

D

D

R

G

K

R

L

F

F

F

A

R

F

L

P

E

E

D

H

H

L

L

Q

N

R
|
R

L

L

K

E

N

A

S

S

L

C

H

K

A

K

T

M

S

R

I

L

Q

R

N

M

P

P

L

L

T

P

D

R

A

E

E

E

W

V

L

I

E

K

L

T

L

L

S

V

T

D

L

M

I

V

E

A

K

K

H

S

P

G

W

I

S

R

D

D

Q

A

Y
x
F

I

V

Y

E

L

L

Q

I

I

V

T

T

R

R

G

G

I

L

Q

T

I

G

Q

V

R

R

D

G

H

A

L

K

P

P

E

E

D

E

G

L

L

L

I

N

P

N

T

N

I

L

Y

Y

A

M

Y

F

S

I

N

Q

P

P

L

Y

K

V

A

W

V

V

S

M

D

D

-

P

D

D

I

V

I

Q

E

Y

N

T

G

G

I

G

S

R

A

A

I

I

T

V

L

A

E

R

D

T

I

V

R

R

W

R

-

V

-

P

-

G

-

S

L

I

N

D

C

P

D

T

I

I

K
|
K

A

N

I

L

T

Q

L

W

L

G

P

D

N

L

I

V

M

R

M

G

K

L

L

F

T

E

A

A

K

N

A

D

R

R

H

G

A

A

D

T

D

Y

A

P

I

F

L

L

V

T

S

D

R

G

E

D

G

A

F

N

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S
x
F

N
|
N

C

V

F

V

I

L

V

I

K

K

N

D

G

G

T

V

L

L

I

Y

T

T

P

P

P

D

N

R

G

G

H

-

S

-

L

V

L
|
L

P

Q

G
|
G

V
x
I

T
|
T

R

R

M

K

V

S

I

V

E

I

R

D

V

A

A

A

I

R

A

S

H

C

N

G

I

Y

P

E

M

I

L

R

E

V

Q

E

Q

H

I

V

T

P

L

I

T

E

D

A

L

T

E

Y

S

Q

A

A

D

D

E

E

I

I

W

L

L

M

S

C

S
x
T

T
x
A

K

G

D

G

A

I

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

D

S

K

A

P

V

V

G

K

T

D

G

G

K

K

P

V

G

G

-

P

-

I

-

T

-

K

A

A

V

I

W

W

Q

D

K

R

M

Y

R

W

G

A

F

M

Y

H

Q

W

E

E

H

D

K

E

L

F

A

S

F

F

active site: Y58 (= Y39), V60 (= V41), K179 (= K154), E212 (= E187), L234 (= L211)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: V60 (= V41), R77 (= R58), F113 (≠ Y94), K179 (= K154), E212 (= E187), G215 (≠ S190), F216 (≠ S191), N217 (= N192), L234 (= L211), G236 (= G213), I237 (≠ V214), T238 (= T215), T273 (≠ S250), T274 (≠ S251), A275 (≠ T252)

4cmdA The (r)-selective transaminase from nectria haematococca (see paper)
27% identity, 98% coverage: 2:289/295 of query aligns to 21:316/322 of 4cmdA

query
sites
4cmdA

N

S

T

K

N

N

P

P

I

L

T

A

E

K

Q

G

I

V

V

A

Y

W

L

I

N

Q

G

G

Q

E

Y

L

M

V

P

P

M

L

L

H

E

E

S

A

K

R

I

I

S

P

T

L

Q

L

D

D

R

Q

G

G

F

F

L

M

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V
|
V

I

P

P

S

V

V

Y

W

D

D

R

G

K

R

L

F

F

F

A

R

F

L

P

E

E

D

H

H

L

L

Q

N

R
|
R

L

L

K

E

N

A

S

S

L

C

H

K

A

K

T

M

S

R

I

L

Q

R

N

M

P

P

L

L

T

P

D

R

A

E

E

E

W

V

L

I

E

K

L

T

L

L

S

V

T

D

L

M

I

V

E

A

K

K

H

S

P

G

W

I

S

R

D

D

Q

A

Y

F

I

V

Y

E

L

L

Q

I

I

V

T

T

R

R

G

G

I

L

Q

T

I

G

Q

V

R

R

D

G

H

A

L

K

P

P

E

E

D

E

G

L

L

L

I

N

P

N

T

N

I

L

Y

Y

A

M

Y

F

S

I

N

Q

P

P

L

Y

K

V

A

W

V

V

S

M

D

D

-

P

D

D

I

V

I

Q

E

Y

N

T

G

G

I

G

S

R

A

A

I

I

T

V

L

A

E

R

D

T

I

V

R

R

W

R

-

V

-

P

-

G

-

S

L

I

N

D

C

P

D

T

I

I

K
|
K

A

N

I

L

T

Q

L

W

L

G

P

D

N

L

I

V

M

R

M

G

K

L

L

F

T

E

A

A

K

N

A

D

R

R

H

G

A

A

D

T

D

Y

A

P

I

F

L

L

V

T

S

D

R

G

E

D

G

A

F

N

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S
x
F

N

N

C

V

F

V

I

L

V

I

K

K

N

D

G

G

T

V

L

L

I

Y

T

T

P

P

P

D

N

R

G

G

H

-

S

-

L

V

L
|
L

P

Q

G
|
G

V
x
I

T
|
T

R

R

M

K

V

S

I

V

E

I

R

D

V

A

A

A

I

R

A

S

H

C

N

G

I

Y

P

E

M

I

L

R

E

V

Q

E

Q

H

I

V

T

P

L

I

T

E

D

A

L

T

E

Y

S

Q

A

A

D

D

E

E

I

I

W

L

L

M

S

C

S

T

S
x
T

T

A

K

G

D

G

A

I

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

D

S

K

A

P

V

V

G

K

T

D

G

G

K

K

P

V

G

G

-

P

-

I

-

T

-

K

A

A

V

I

W

W

Q

D

K

R

M

Y

R

W

G

A

F

M

Y

H

Q

W

E

E

H

D

K

E

L

F

A

S

F

F

active site: Y58 (= Y39), V60 (= V41), K179 (= K154), E212 (= E187), L234 (= L211)
binding pyridoxal-5'-phosphate: R77 (= R58), K179 (= K154), E212 (= E187), G215 (≠ S190), F216 (≠ S191), L234 (= L211), G236 (= G213), I237 (≠ V214), T238 (= T215), T274 (≠ S251)

6xu3A (R)-selective amine transaminase from shinella sp. (see paper)
28% identity, 96% coverage: 4:285/295 of query aligns to 21:306/320 of 6xu3A

query
sites
6xu3A

N

N

P

P

I

F

T

A

E

K

Q

G

I

I

V

A

Y

Y

L

I

N

A

G

G

Q

E

Y

Y

M

V

P

P

M

L

L

H

E

E

S

A

K

R

I

I

S

P

T

I

Q

L

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V

V

I

P

P

A

V

V

Y

W

D

N

R

G

K

R

L

F

F

F

A

R

F

L

P

E

E

D

H

H

L

L

Q

D

R
|
R

L

F

K

E

N

K

S

S

L

C

H

A

A

Q

T

L

S

R

I

L

Q

K

N

S

P

P

L

L

T

S

D

R

A

E

E

E

W

I

L

R

E

D

L

R

L

L

S

V

T

E

L

M

I

T

E

V

K

K

H

S

P

G

W

I

S

R

D

D

Q

A

Y

Y

I

V

Y

M

L

M

Q

I

I

V

T

T

R

R

G

G

I

L

Q

R

I

F

Q

V

R

R

D

Q

H

Y

L

A

P

P

E

E

D

E

G

-

L

C

I

D

P

N

T

F

I

C

Y

Y

A

L

Y

M

S

V

N

M

P

P

L

Y

K

L

A

W

V

V

S

M

D

D

D

E

I

A

I

T

E

Q

-

K

N

N

G

G

I

G

S

S

A

A

I

V

T

I

L

T

E

R

D

T

I

V

R

R

-

R

-

V

-

P

-

G

W

A

L

I

N

D

C

P

D

T

I

V

K
|
K

A

N

I

L

T

Q

L

W

L

G

P

D

N
x
F

I

T

M

R

M

G

K

L

L

M

T

E

A

A

K

R

A

D

R

R

H

G

A

A

D

M

D

Y

A

P

I

I

L

L

V

T

S

D

R

G

E

D

G

A

F

N

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S
x
F

N

N

C

V

F

I

I

L

V

I

K

K

N

D

G

G

T

K

L

L

I

Y

T

T

P

P

P

R

N

K

G

G

H

-

S

-

L

V

L
|
L

P

E

G
|
G

V
|
V

T
|
T

R

R

M

K

V

S

I

V

E

L

R

A

V

V

A

A

I

E

A

K

H

L

N

G

I

Y

P

P

M

Y

L

T

E

I

Q

D

I

V

T

P

L

V

T

E

D

L

L

A

E

Y

S

Q

A

C

D

D

E

E

I

I

W

L

L

F

S

V

S

T

S
x
T

T

A

K

G

D

G

A

V

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

G

S

Q

A

P

V

V

G

G

T

D

G

G

K

Q

P

V

G

G

A

P

V

I

W

S

Q

K

K

A

M

L

-

W

R

K

G

G

F

Y

Y

W

Q

D

E

A

H

H

active site: Y56 (= Y39), K176 (= K154), E209 (= E187), L231 (= L211)
binding pyridoxal-5'-phosphate: R75 (= R58), K176 (= K154), F183 (≠ N161), E209 (= E187), G212 (≠ S190), F213 (≠ S191), L231 (= L211), G233 (= G213), V234 (= V214), T235 (= T215), T271 (≠ S251)

6xu3C (R)-selective amine transaminase from shinella sp. (see paper)
28% identity, 96% coverage: 4:285/295 of query aligns to 23:308/322 of 6xu3C

query
sites
6xu3C

N

N

P

P

I

F

T

A

E

K

Q

G

I

I

V

A

Y

Y

L

I

N

A

G

G

Q

E

Y

Y

M

V

P

P

M

L

L

H

E

E

S

A

K

R

I

I

S

P

T

I

Q

L

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V
|
V

I

P

P

A

V

V

Y

W

D

N

R

G

K

R

L

F

F

F

A

R

F

L

P

E

E

D

H

H

L

L

Q

D

R
|
R

L

F

K

E

N

K

S

S

L

C

H

A

A

Q

T

L

S

R

I

L

Q

K

N

S

P

P

L

L

T

S

D

R

A

E

E

E

W

I

L

R

E

D

L

R

L

L

S

V

T

E

L

M

I

T

E

V

K

K

H

S

P

G

W

I

S

R

D

D

Q

A

Y

Y

I

V

Y

M

L

M

Q

I

I

V

T

T

R

R

G

G

I

L

Q

R

I

F

Q

V

R

R

D

Q

H

Y

L

A

P

P

E

E

D

E

G

-

L

C

I

D

P

N

T

F

I

C

Y

Y

A

L

Y

M

S

V

N

M

P

P

L

Y

K

L

A

W

V

V

S

M

D

D

D

E

I

A

I

T

E

Q

-

K

N

N

G

G

I

G

S

S

A

A

I

V

T

I

L

T

E

R

D

T

I

V

R

R

-

R

-

V

-

P

-

G

W

A

L

I

N

D

C

P

D

T

I

V

K
|
K

A

N

I

L

T

Q

L

W

L

G

P

D

N
x
F

I

T

M

R

M

G

K

L

L

M

T

E

A

A

K

R

A

D

R

R

H

G

A

A

D

M

D

Y

A

P

I

I

L

L

V

T

S

D

R

G

E

D

G

A

F

N

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S

F

N
|
N

C

V

F

I

I

L

V

I

K

K

N

D

G

G

T

K

L

L

I

Y

T

T

P

P

P

R

N

K

G

G

H

-

S

-

L

V

L
|
L

P

E

G
|
G

V
|
V

T
|
T

R

R

M

K

V

S

I

V

E

L

R

A

V

V

A

A

I

E

A

K

H

L

N

G

I

Y

P

P

M

Y

L

T

E

I

Q

D

I

V

T

P

L

V

T

E

D

L

L

A

E

Y

S

Q

A

C

D

D

E

E

I

I

W

L

L

F

S

V

S
x
T

T
x
A

K

G

D

G

A

V

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

G

S

Q

A

P

V

V

G

G

T

D

G

G

K

Q

P

V

G

G

A

P

V

I

W

S

Q

K

K

A

M

L

-

W

R

K

G

G

F

Y

Y

W

Q

D

E

A

H

H

active site: Y58 (= Y39), K178 (= K154), E211 (= E187), L233 (= L211)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: V60 (= V41), R77 (= R58), K178 (= K154), F185 (≠ N161), E211 (= E187), G214 (≠ S190), N216 (= N192), L233 (= L211), G235 (= G213), V236 (= V214), T237 (= T215), T272 (≠ S250), T273 (≠ S251), A274 (≠ T252)

6xu3B (R)-selective amine transaminase from shinella sp. (see paper)
28% identity, 96% coverage: 4:285/295 of query aligns to 22:307/321 of 6xu3B

query
sites
6xu3B

N

N

P

P

I

F

T

A

E

K

Q

G

I

I

V

A

Y

Y

L

I

N

A

G

G

Q

E

Y

Y

M

V

P

P

M

L

L

H

E

E

S

A

K

R

I

I

S

P

T

I

Q

L

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V

V

I

P

P

A

V

V

Y

W

D

N

R

G

K

R

L

F

F

F

A

R

F

L

P

E

E

D

H

H

L

L

Q

D

R
|
R

L

F

K

E

N

K

S

S

L

C

H

A

A

Q

T

L

S

R

I

L

Q

K

N

S

P

P

L

L

T

S

D

R

A

E

E

E

W

I

L

R

E

D

L

R

L

L

S

V

T

E

L

M

I

T

E

V

K

K

H

S

P

G

W

I

S

R

D

D

Q

A

Y
|
Y

I

V

Y

M

L

M

Q

I

I

V

T

T

R

R

G

G

I

L

Q

R

I

F

Q

V

R

R

D

Q

H

Y

L

A

P

P

E

E

D

E

G

-

L

C

I

D

P

N

T

F

I

C

Y

Y

A

L

Y

M

S

V

N

M

P

P

L

Y

K

L

A

W

V

V

S

M

D

D

D

E

I

A

I

T

E

Q

-

K

N

N

G

G

I

G

S

S

A

A

I

V

T

I

L

T

E

R

D

T

I

V

R

R

-

R

-

V

-

P

-
x
P

-

G

W

A

L

I

N
x
D

C

P

D

T

I

V

K
|
K

A

N

I

L

T

Q

L

W

L

G

P

D

N
x
F

I

T

M

R

M

G

K

L

L

M

T

E

A

A

K

R

A

D

R

R

H

G

A

A

D

M

D

Y

A

P

I

I

L

L

V

T

S

D

R

G

E

D

G

A

F

N

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S
x
F

N
|
N

C

V

F

I

I

L

V

I

K

K

N

D

G

G

T

K

L

L

I

Y

T

T

P

P

P

R

N
x
K

G

G

H

-

S

-

L

V

L
|
L

P

E

G
|
G

V
|
V

T
|
T

R

R

M

K

V

S

I

V

E

L

R

A

V

V

A

A

I

E

A

K

H

L

N

G

I

Y

P

P

M

Y

L

T

E

I

Q

D

I

V

T

P

L

V

T

E

D

L

L

A

E

Y

S

Q

A

C

D

D

E

E

I

I

W

L

L

F

S

V

S

T

S
x
T

T

A

K

G

D

G

A

V

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

G

S

Q

A

P

V

V

G

G

T

D

G

G

K

Q

P

V

G

G

A

P

V

I

W

S

Q

K

K

A

M

L

-

W

R

K

G

G

F

Y

Y

W

Q

D

E

A

H

H

active site: Y57 (= Y39), K177 (= K154), E210 (= E187), L232 (= L211)
binding 3-aminobenzoic acid: P169 (vs. gap), D173 (≠ N150), K229 (≠ N206)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: R76 (= R58), Y112 (= Y94), K177 (= K154), F184 (≠ N161), E210 (= E187), G213 (≠ S190), F214 (≠ S191), N215 (= N192), L232 (= L211), G234 (= G213), V235 (= V214), T236 (= T215), T272 (≠ S251)

6fteB Crystal structure of an (r)-selective amine transaminase from exophiala xenobiotica
27% identity, 98% coverage: 2:289/295 of query aligns to 21:316/321 of 6fteB

query
sites
6fteB

N

S

T

T

N

N

P

P

I

F

T

A

E

K

Q

G

I

V

V

A

Y

W

L

V

N

E

G

G

Q

K

Y

L

M

V

P

P

M

V

L

N

E

E

S

A

K

R

I

I

S

P

T

L

Q

M

D

D

R

Q

G

G

F

F

L

L

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V

V

I

P

P

S

V

V

Y

W

D

D

R

G

K

R

L

F

F

F

A

R

F

L

P

D

E

D

H

H

L

L

Q

D

R
|
R

L

F

K

E

N

L

S

S

L

C

H

S

A

K

T

M

S

R

I

F

Q

K

N

M

P

P

L

L

T

P

D

R

A

Q

E

E

W

V

L

K

E

R

L

I

L

L

S

V

T

D

L

M

I

V

E

A

K

K

H

S

P

G

W

I

S

K

D

D

Q

A

Y

F

I

V

Y

E

L

I

Q

I

I

V

T

T

R

R

G

G

I

L

Q

K

I

G

Q

V

R

R

D

G

H

L

L

K

P

A

E

G

D

E

G

S

L

L

I

T

P

N

T

N

I

L

Y

Y

A

M

Y

W

S

I

N

Q

P

P

L

Y

K

I

A

W

V

V

S

M

D

E

-

P

D

E

I

M

I

Q

E

R

N

T

G

G

I

G

S

S

A

A

I

I

T

I

L

A

E

R

D

T

I

V

R

K

W

R

-

T

-

S

-

P

-

G

-

S

L

M

N

D

C

P

D

T

I

V

K
|
K

A

N

I

L

T

Q

L

W

L

G

P

D

N

L

I

T

M

R

M

G

K

M

L

L

T

E

A

A

K

Q

A

D

R

R

H

G

A

A

D

D

D

Y

A

P

I

F

L

L

V

T

S

D

R

G

E

D

G

G

F

N

I

I

T

T

E
|
E

G

G

T

S

S

G

S
x
F

N
|
N

C

I

F

V

I

F

V

I

K

K

N

D

G

G

T

V

L

L

I

Y

T

T

P

P

P

D

N

R

G

G

H

-

S

-

L

V

L
|
L

P

K

G
|
G

V
|
V

T
|
T

R

R

M

K

V

S

I

V

E

A

R

D

V

A

A

A

I

K

A

A

H

N

N

G

I

I

P

E

M

M

L

R

E

I

Q

E

Q

F

I

V

T

P

L

T

T

E

D

M

L

A

E

Y

S

Q

A

C

D

D

E

E

I

C

W

F

L

M

S

C

S

T

S
x
T

T

A

K

G

D

G

A

V

L

M

P

P

V

I

T

T

R

S

L

M

N

D

D

G

S

Q

A

P

V

I

G

G

T

D

G

G

K

K

P

V

G

G

A

P

V

V

-

T

-

K

-

E

-

I

W

W

Q

D

K

G

M

Y

R

W

G

A

F

M

Y

H

Q

Y

E

D

H

D

K

K

L

Y

A

S

F

F

active site: Y58 (= Y39), K179 (= K154), E212 (= E187), L234 (= L211)
binding pyridoxal-5'-phosphate: R77 (= R58), K179 (= K154), E212 (= E187), F216 (≠ S191), N217 (= N192), L234 (= L211), G236 (= G213), V237 (= V214), T238 (= T215), T274 (≠ S251)

7dbeB Structure of a novel transaminase
27% identity, 95% coverage: 2:282/295 of query aligns to 32:316/332 of 7dbeB

query
sites
7dbeB

N

R

T

T

N

S

P

P

I

L

T

A

E

G

Q

G

I

C

V

A

Y

W

L

I

N

E

G

G

Q

A

Y

F

M

V

P

P

M

A

L

A

E

A

S

A

K

R

I

I

S

S

T

I

Q

F

D

D

R

A

G

G

F

F

L

G

F

H

G

S

D

D

G

V

V

T

Y

Y

E

T

V

V

I

A

P

H

V

V

Y

W

D

H

R

G

K

N

L

F

F

F

A

R

F

L

P

E

E

D

H

H

L

V

Q

E

R
|
R

L

F

K

L

N

A

S

G

L

A

H

E

A

K

T

M

S

R

I

I

Q

P

N

M

P

P

L

A

T

T

D

K

A

A

E

E

W

I

L

M

E

D

L

L

L

M

S

R

T

G

L

C

I

V

E

S

K

K

H

S

P

G

W

L

S

R

D

E

Q

A

Y

Y

I

V

Y

N

L

V

Q

C

I

V

T

T

R

R

G

G

I

Y

Q

G

I

R

Q

K

R

P

D

G

H

E

L

K

P

T

E

L

D

E

G

A

L

L

I

E

P

S

T

Q

I

L

Y

Y

A

V

Y

Y

S

A

N

I

P

P

L

Y

K

L

A

W

V

V

S

F

D

S

D

P

I

I

I

R

E

Q

-

I

N

E

G

G

I

I

S

D

A

A

I

V

T

I

L

A

E

Q

D

S

I

V

R

R

W

R

L

S

N

P

C

A

D

N

-

V

-

M

-

D

-

P

-

W

I

I

K
|
K

A

N

I

Y

T

Q

L

W

L

G

P

D

N

L

I

V

M

R

M

A

K

T

L

F

T

E

A

A

K

Q

A

E

R

R

H

G

A

A

D

R

D

T

A

A

I

F

L

L

V

L

S

D

R

S

E

D

G

G

F

F

I

V

T

T

E
|
E

G

G

T

P

S
x
G

S
x
F

N
|
N

C

V

F

L

I

M

V

V

K

K

N

D

G

G

T

T

L

V

I

F

T

T

P

-

N

A

G

A

H

R

S

N

L

V

L
|
L

P

P

G
|
G

V
x
I

T
|
T

R

R

M

R

V

T

I

A

E

L

R

E

V

I

A

A

I

R

A

D

H

F

N

G

I

L

P

Q

M

T

L

V

E

I

Q

G

Q

D

I

V

T

T

L

P

T

E

D

M

L

L

E

R

S

G

A

A

D

D

E

E

I

I

W

F

L

A

S

A

S

T

S
x
T

T

A

K

G

D

G

A

V

L

T

P

P

V

V

T

V

R

A

L

L

N

D

D

G

S

A

A

P

V

V

G

G

T

A

G

G

K

V

P

P

G

G

A

D

V

W

W

T

Q

R

K

K

M

I

R

R

G

T

F

R

Y

Y

binding pyridoxal-5'-phosphate: R88 (= R58), K190 (= K154), E223 (= E187), G226 (≠ S190), F227 (≠ S191), N228 (= N192), L245 (= L211), G247 (= G213), I248 (≠ V214), T249 (= T215), T285 (≠ S251)

8onjA Crystal structure of d-amino acid aminotransferase from aminobacterium colombiense point mutant r88l (see paper)
30% identity, 93% coverage: 10:282/295 of query aligns to 5:268/277 of 8onjA

query
sites
8onjA

I

L

V

C

Y

Y

L

I

N

D

G

G

Q

K

Y

F

M

L

P

P

M

L

L

E

E

E

S

A

K

K

I

L

S

P

T

V

Q

T

D

D

R

L

G

I

F

I

L

Q

F

R

G

G

D

V

G

G

V

V

Y

F

E

E

V

T

I

I

P

S

V

T

Y

H

D

S

R

R

K

R

L

P

F

L

A

M

F

L

P

T

E

P

H

H

L

L

Q

K

R
|
R

L

L

K

E

N

G

S

S

L

A

H

T

A

A

T

S

S

S

I

I

Q

V

N

M

P

P

L

A

T

T

D

L

A

D

E

E

W

M

L

A

E

R

L

I

S

R

T

E

L

G

I

I

E

K

K

K

H

M

P

-

W

G

S

C

D

E

Q

T

Y

M

I

V

Y

L

L

P

Q

Y

I

I

T

T

R

G

G

G

I

D

Q

S

I

F

Q

G

R

K

D

D

H

H

L

L

P

F

E

S

D

S

G

S

L

R

I

Y

P

F

T

V

I

I

Y

F

A

-

Y

-

S

-

N

E

P

E

L

I

K

R

A

K

V

P

S

D

D

P

D

I

I

L

I

Y

E

E

N

K

G

G

I

V

S

A

A

L

I

H

T

P

L

I

E

N

D

A

I

E

R
|
R

W

Y

L

L

N

P

C

-

D

S

I

T

K
|
K

A

S

I

I

T

-

L

-

P

-

N

N

I

Y

M

M

M

L

K

S

L

F

T

T

A

G

K

Q

A

-

R

R

H

D

A

S

D

K

D

G

A

A

-

Y

-

E

I

I

L

L

V

Y

S

C

R

P

E

E

G

G

F

E

I

I

T

V

E
|
E

G

G

T

S

S
x
H

S
|
S

N

T

C

F

F

F

I

L

V

I

K

K

N

N

G

G

T

H

L

L

I

I

T

T

P

A

P

P

N

T

G

S

H

R

S

A

L
|
L

L

-

P

S

G

G

V
x
T

T
|
T

R

R

M

Q

V

I

I

V

E

L

R

E

V

L

A

A

I

R

A

R

H

G

N

N

I

I

P

Q

M

V

L

E

E

E

Q

R

Q

C

I

P

T

L

L

L

T

T

D

E

L

L

E

P

S

E

A

A

D

E

E

E

I

A

W

F

L

I

S

T

S
x
G

S
x
T

T

V

K

K

D

E

A

L

L

L

P

P

V

V

T

V

R

R

L

I

N

G

D

D

S

Q

A

I

V

I

G

G

T

N

G

G

K

V

P

P

G

G

A

K

V

L

W

T

Q

K

K

H

M

L

R

H

G

Q

F

V

Y

Y

binding pyridoxal-5'-phosphate: R53 (= R58), R138 (= R147), K144 (= K154), E174 (= E187), H177 (≠ S190), S178 (= S191), L197 (= L210), T200 (≠ V214), T201 (= T215), G236 (≠ S250), T237 (≠ S251)

4ce5B First crystal structure of an (r)-selective omega-transaminase from aspergillus terreus (see paper)
27% identity, 96% coverage: 2:285/295 of query aligns to 23:310/325 of 4ce5B

query
sites
4ce5B

N

T

T

T

N

N

P

P

I

F

T

A

E

K

Q

G

I

I

V

A

Y

W

L

V

N

E

G

G

Q

E

Y

L

M

V

P

P

M

L

L

A

E

E

S

A

K

R

I

I

S

P

T

L

Q

L

D

D

R

Q

G

G

F

F

L

M

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V
|
V

I

P

P

S

V

V

Y

W

D
|
D

R

G

K
x
R

L

F

F

F

A

R

F

L

P

D

E

D

H

H

L

I

Q

T

R
|
R

L

L

K

E

N

A

S

S

L

C

H

T

A

K

T

L

S

R

I

L

Q

R

N

L

P

P

L

L

T

P

D

R

A

D

E

Q

W

V

L

K

E

Q

L

I

L

L

S

V

T

E

L

M

I

V

E

A

K

K

H

S

P

G

W

I

S

R

D

D

Q

A

Y

F

I

V

Y

E

L

L

Q

I

I

V

T

T

R

R

G

G

I

L

Q

K

I

G

Q

V

R

R

D

G

H

T

L

R

P

P

E

E

D

D

G

-

L

I

I

V

P

N

T

N

I

L

Y

Y

A

M

Y

F

S

V

N

Q

P

P

L

Y

K

V

A

W

V

V

S

M

D

E

-

P

D

D

I

M

I

Q

E

R

N

V

G

G

I

G

S

S

A

A

I

V

T

V

L

A

E

R

D

T

I

V

R

R

-

R

-

V

-

P

-

G

W

A

L

I

N

D

C

P

D

T

I

V

K
|
K

A

N

I

L

T

Q

L
x
W

L

G

P

D

N

L

I

V

M

R

M

G

K

M

L

F

T

E

A

A

K

A

A

D

R

R

H

G

A

A

D

T

D

Y

A

P

I

F

L

L

V

T

S

D

R

G

E

D

G

A

F

H

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S
x
F

N
|
N

C

I

F

V

I

L

V

V

K

K

N

D

G

G

T

V

L

L

I

Y

T

T

P

P

P

D

N

R

G

G

H

-

S

-

L

V

L
|
L

P

Q

G
|
G

V
|
V

T
|
T

R

R

M

K

V

S

I

V

E

I

R

N

V

A

A

A

I

E

A

A

H

F

N

G

I

I

P

E

M

V

L

R

E

V

Q

E

Q

F

I

V

T

P

L

V

T

E

D

L

L

A

E

Y

S

R

A

C

D

D

E

E

I

I

W

F

L

M

S

C

S
x
T

T
x
A

K

G

D

G

A

I

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

G

S

M

A

P

V

V

G

N

T

G

G

G

K

Q

P

I

G

G

A

P

V

I

W

T

Q

K

K

K

M

I

-

W

R

D

G

G

F

Y

Y

W

Q

A

E

M

H

H

active site: Y60 (= Y39), K180 (= K154), E213 (= E187), L235 (= L211)
binding calcium ion: D67 (= D46), R69 (≠ K48)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-d-glutamic acid: V62 (= V41), R79 (= R58), K180 (= K154), W184 (≠ L158), E213 (= E187), G216 (≠ S190), F217 (≠ S191), N218 (= N192), L235 (= L211), G237 (= G213), V238 (= V214), T239 (= T215), T274 (≠ S250), T275 (≠ S251), A276 (≠ T252)
binding pyridoxal-5'-phosphate: R79 (= R58), K180 (= K154), E213 (= E187), G216 (≠ S190), F217 (≠ S191), L235 (= L211), G237 (= G213), V238 (= V214), T239 (= T215), T275 (≠ S251)

4ce5A First crystal structure of an (r)-selective omega-transaminase from aspergillus terreus (see paper)
27% identity, 96% coverage: 2:285/295 of query aligns to 23:310/325 of 4ce5A

query
sites
4ce5A

N

T

T

T

N

N

P

P

I

F

T

A

E

K

Q

G

I

I

V

A

Y

W

L

V

N

E

G

G

Q

E

Y

L

M

V

P

P

M

L

L

A

E

E

S

A

K

R

I

I

S

P

T

L

Q

L

D

D

R

Q

G

G

F

F

L

M

F

H

G

S

D

D

G

L

V

T

Y
|
Y

E

D

V
|
V

I

P

P

S

V

V

Y

W

D

D

R

G

K

R

L

F

F

F

A

R

F

L

P

D

E

D

H

H

L

I

Q

T

R
|
R

L

L

K

E

N

A

S

S

L

C

H

T

A

K

T

L

S

R

I

L

Q

R

N

L

P

P

L

L

T

P

D

R

A

D

E

Q

W

V

L

K

E

Q

L

I

L

L

S

V

T

E

L

M

I

V

E

A

K

K

H

S

P

G

W

I

S

R

D

D

Q

A

Y

F

I

V

Y

E

L

L

Q

I

I

V

T

T

R

R

G

G

I

L

Q

K

I

G

Q

V

R

R

D

G

H

T

L

R

P

P

E

E

D

D

G

-

L

I

I

V

P

N

T

N

I

L

Y

Y

A

M

Y

F

S

V

N

Q

P

P

L

Y

K

V

A

W

V

V

S

M

D

E

-

P

D

D

I

M

I

Q

E

R

N

V

G

G

I

G

S

S

A

A

I

V

T

V

L

A

E

R

D

T

I

V

R

R

-

R

-

V

-

P

-

G

W

A

L

I

N

D

C

P

D

T

I

V

K
|
K

A

N

I

L

T

Q

L
x
W

L

G

P

D

N

L

I

V

M

R

M

G

K

M

L

F

T

E

A

A

K

A

A

D

R

R

H

G

A

A

D

T

D

Y

A

P

I

F

L

L

V

T

S

D

R

G

E

D

G

A

F

H

I

L

T

T

E
|
E

G

G

T

S

S
x
G

S
x
F

N
|
N

C

I

F

V

I

L

V

V

K

K

N

D

G

G

T

V

L

L

I

Y

T

T

P

P

P

D

N

R

G

G

H

-

S

-

L

V

L
|
L

P

Q

G
|
G

V
|
V

T
|
T

R

R

M

K

V

S

I

V

E

I

R

N

V

A

A

A

I

E

A

A

H

F

N

G

I

I

P

E

M

V

L

R

E

V

Q

E

Q

F

I

V

T

P

L

V

T

E

D

L

L

A

E

Y

S

R

A

C

D

D

E

E

I

I

W

F

L

M

S

C

S
x
T

T

A

K

G

D

G

A

I

L

M

P

P

V

I

T

T

R

T

L

L

N

D

D

G

S

M

A

P

V

V

G

N

T

G

G

G

K

Q

P

I

G

G

A

P

V

I

W

T

Q

K

K

K

M

I

-

W

R

D

G

G

F

Y

Y

W

Q

A

E

M

H

H

active site: Y60 (= Y39), K180 (= K154), E213 (= E187), L235 (= L211)
binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-d-glutamic acid: Y60 (= Y39), V62 (= V41), R79 (= R58), K180 (= K154), W184 (≠ L158), E213 (= E187), G216 (≠ S190), F217 (≠ S191), N218 (= N192), L235 (= L211), G237 (= G213), V238 (= V214), T239 (= T215), T274 (≠ S250), T275 (≠ S251)
binding pyridoxal-5'-phosphate: R79 (= R58), K180 (= K154), E213 (= E187), F217 (≠ S191), L235 (= L211), G237 (= G213), V238 (= V214), T239 (= T215), T275 (≠ S251)

Query Sequence

>WP_019556825.1 NCBI__GCF_000381085.1:WP_019556825.1
MNTNPITEQIVYLNGQYMPMLESKISTQDRGFLFGDGVYEVIPVYDRKLFAFPEHLQRLK
NSLHATSIQNPLTDAEWLELLSTLIEKHPWSDQYIYLQITRGIQIQRDHLPEDGLIPTIY
AYSNPLKAVSDDIIENGISAITLEDIRWLNCDIKAITLLPNIMMKLTAKARHADDAILVS
REGFITEGTSSNCFIVKNGTLITPPNGHSLLPGVTRMVIERVAIAHNIPMLEQQITLTDL
ESADEIWLSSSTKDALPVTRLNDSAVGTGKPGAVWQKMRGFYQEHKLAFIDEQAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory