SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_019557177.1 NCBI__GCF_000381085.1:WP_019557177.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
43% identity, 60% coverage: 37:126/151 of query aligns to 15:104/200 of 6j2lA

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6j2lA

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binding zinc ion: D79 (= D101), D81 (= D103), D83 (= D105), C96 (= C118), C103 (= C125)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
37% identity, 69% coverage: 27:130/151 of query aligns to 4:107/204 of 7bgnA

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7bgnA

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binding adenosine monophosphate: W60 (= W83), K62 (= K85), G63 (= G86), T65 (≠ E88), S66 (= S89), T94 (≠ A117), H96 (= H119)
binding zinc ion: D78 (= D101), D80 (= D103), D82 (= D105), C95 (= C118), C102 (= C125)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
34% identity, 75% coverage: 27:139/151 of query aligns to 6:127/213 of 7bgmA

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binding zinc ion: D80 (= D101), D82 (= D103), D84 (= D105), C97 (= C118), C104 (= C125)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lB Crystal structure of bi-functional enzyme (see paper)
40% identity, 60% coverage: 37:126/151 of query aligns to 14:97/185 of 6j2lB

query
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6j2lB

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binding zinc ion: D78 (= D101), D80 (= D103), D82 (= D105)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>WP_019557177.1 NCBI__GCF_000381085.1:WP_019557177.1
MCQATGSPLSFKQLEKAKSGDHFDWQTLKSQVRFDQNGLIPAIAQQFDTKEVLMMAWMNM
QSLQETLDTGRVCYWSRSRQNYWRKGEESGQIQRLKALAFDCDGDTILLSVDQTGPACHS
GRRSCFYTNVQGSDVEITSNPLIDPNELYKK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory