SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_019557256.1 NCBI__GCF_000381085.1:WP_019557256.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
38% identity, 64% coverage: 5:76/112 of query aligns to 114:178/200 of 6j2lA

query
sites
6j2lA

L

L

K

Y

Q

Q

L

L

D

E

A

Q

V

L

L

L

E

A

A

E

R

R

K

K

L

Y

E

A

S

D

A

P

D

E

S

T

S

S

Y

Y

V

T

A

A

S

K

L

L

Y

Y

S

A

K

S

G

G

T

T

E

K

K

R

I

I

L

A

K

Q

K

K

I

V

G

G

E

E

E
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E

A

G

N

V

E

E

T

T

I

A

M

L

A

A

A

A

K

-

D

-

L

-

E

-

H

-

V

-

N

-

N

T

L

V

E

H

N

D

K

R

E

F

H

E

L

L

V

T

Y

N

E
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E

I

A

A

S

D
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D

L

L

W

M

F

Y

H

H

binding zinc ion: E152 (= E43), E171 (= E69), D174 (= D72)

Sites not aligning to the query:

binding zinc ion: 79, 81, 83, 96, 103

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
34% identity, 74% coverage: 17:99/112 of query aligns to 121:204/204 of 7bgnA

query
sites
7bgnA

L
|
L

E

E

S

S

A

T

D

I

S

S

S

Q

Y
x
R

V

K

A

A

S

E

L

V

Y

V

S

P

K
x
S

G
x
W

T
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T

E

K

K

R

I

L

L

L

-

L

-

N

-

D

-

K

-

L

-

L

-

C

K

S

K

K

I

I

G

R

E

E

E
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E

A

A

N

N

E

E

T

L

I

C

M

-

A

-

A

-

K

-

D

-

L

-

E

E

H

T

V

L

N

E

N

N

L

N

E

E

N

D

K

K

E

S

H

R

L

T

V

A

Y

S

E
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E

I

M

A

A

D
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D

L

V

W

L

F
x
Y

H

H

T

A

M

M

V

V

L

L

L

L

A

A

S

L

Q

K

N

D

L

V

S

K

S

V

E

E

D

E

I

V

T

L

N

Q

E

V

L

L

Q

R

R

Q

R
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R

F
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F

binding adenosine monophosphate: L121 (= L17), R128 (≠ Y24), S135 (≠ K31), W136 (≠ G32), T137 (= T33), Y180 (≠ F75), R203 (= R98), F204 (= F99)
binding zinc ion: E154 (= E43), E174 (= E69), D177 (= D72)

Sites not aligning to the query:

binding adenosine monophosphate: 60, 62, 63, 65, 66, 94, 96
binding zinc ion: 78, 80, 82, 95, 102

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
38% identity, 57% coverage: 36:99/112 of query aligns to 156:213/213 of 7bgmA

query
sites
7bgmA

I

L

L

C

K

S

K

K

I

I

G

R

E

E

E
|
E

A

A

N

N

E

E

T

L

I

C

M

-

A

-

A

-

K

-

D

-

L

-

E

E

H

T

V

L

N

E

N

N

L

N

E

E

N

D

K

K

E

S

H

R

L

T

V

A

Y

S

E
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E

I

M

A

A

D
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D

L

V

W

L

F

Y

H

H

T

A

M

M

V

V

L

L

L

L

A

A

S

L

Q

K

N

D

L

V

S

K

S

V

E

E

D

E

I

V

T

L

N

Q

E

V

L

L

Q

R

R

Q

R

R

F

F

binding zinc ion: E163 (= E43), E183 (= E69), D186 (= D72)

Sites not aligning to the query:

binding zinc ion: 80, 82, 84, 97, 104

Query Sequence

>WP_019557256.1 NCBI__GCF_000381085.1:WP_019557256.1
MSNVLKQLDAVLEARKLESADSSYVASLYSKGTEKILKKIGEEANETIMAAKDLEHVNNL
ENKEHLVYEIADLWFHTMVLLASQNLSSEDITNELQRRFGLSGHDEKASRDQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory