SitesBLAST
Comparing WP_019557476.1 NCBI__GCF_000381085.1:WP_019557476.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
65% identity, 99% coverage: 1:394/396 of query aligns to 1:390/392 of P33221
- M1 (= M1) modified: Initiator methionine, Removed
- EL 22:23 (= EL 22:23) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- E82 (= E82) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- R114 (= R114) binding ATP
- K155 (= K155) binding ATP
- SSGKGQ 160:165 (= SSGKGQ 160:165) binding ATP
- G162 (= G162) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
- K179 (≠ S179) modified: N6-acetyllysine
- EGVV 195:198 (≠ EGFV 195:198) binding ATP
- E203 (= E203) binding ATP
- E267 (= E270) binding Mg(2+)
- E279 (= E282) binding Mg(2+)
- D286 (= D289) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- K355 (= K359) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
- RR 362:363 (= RR 366:367) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
65% identity, 99% coverage: 4:394/396 of query aligns to 3:387/389 of 1kjiA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E270), E276 (= E282), D283 (= D289), T284 (= T290), R360 (= R367)
- binding phosphomethylphosphonic acid adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), E200 (= E203), Q222 (= Q225), E264 (= E270), F266 (= F272), E276 (= E282)
- binding magnesium ion: E264 (= E270), E276 (= E282)
1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
65% identity, 99% coverage: 4:394/396 of query aligns to 3:387/389 of 1ez1A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E270), E276 (= E282), D283 (= D289), T284 (= T290), R360 (= R367)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), E192 (= E195), V194 (≠ F197), V195 (= V198), F197 (= F200), E200 (= E203), Q222 (= Q225), E264 (= E270), F266 (= F272), E276 (= E282)
- binding glycinamide ribonucleotide: G20 (= G21), E21 (= E22), L22 (= L23), E81 (= E82), I82 (= I83), S160 (= S161), D283 (= D289), K352 (= K359), R359 (= R366), R360 (= R367)
- binding magnesium ion: E264 (= E270), E276 (= E282)
1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
65% identity, 99% coverage: 4:394/396 of query aligns to 3:387/389 of 1eyzA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E264 (= E270), E276 (= E282), D283 (= D289), T284 (= T290), R360 (= R367)
- binding phosphoaminophosphonic acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E192 (= E195), V195 (= V198), F197 (= F200), E200 (= E203), E264 (= E270), F266 (= F272), E276 (= E282)
- binding magnesium ion: E264 (= E270), E276 (= E282)
1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
64% identity, 99% coverage: 4:394/396 of query aligns to 3:386/388 of 1kjqA
- active site: E114 (= E115), K154 (= K155), E263 (= E270), E275 (= E282), D282 (= D289), T283 (= T290), R359 (= R367)
- binding adenosine-5'-diphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), E191 (= E195), V193 (≠ F197), V194 (= V198), F196 (= F200), E199 (= E203), Q221 (= Q225), F265 (= F272), E275 (= E282)
- binding magnesium ion: E263 (= E270), E275 (= E282)
1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
64% identity, 99% coverage: 4:394/396 of query aligns to 3:384/386 of 1kjjA
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E270), E273 (= E282), D280 (= D289), T281 (= T290), R357 (= R367)
- binding phosphothiophosphoric acid-adenylate ester: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), E197 (= E203), Q219 (= Q225), E261 (= E270), F263 (= F272), E273 (= E282)
- binding magnesium ion: E261 (= E270), E273 (= E282)
1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
64% identity, 99% coverage: 4:394/396 of query aligns to 3:384/386 of 1kj8A
- active site: E114 (= E115), K154 (= K155), S159 (= S160), G161 (= G162), E261 (= E270), E273 (= E282), D280 (= D289), T281 (= T290), R357 (= R367)
- binding adenosine-5'-triphosphate: R113 (= R114), I152 (≠ V153), K154 (= K155), S159 (= S160), S160 (= S161), G161 (= G162), Q164 (= Q165), E189 (= E195), V192 (= V198), F194 (= F200), E197 (= E203), Q219 (= Q225), G222 (= G228), E261 (= E270), F263 (= F272), E273 (= E282)
- binding glycinamide ribonucleotide: G20 (= G21), E21 (= E22), L22 (= L23), E81 (= E82), I82 (= I83), S160 (= S161), D280 (= D289), K349 (= K359), R356 (= R366)
- binding magnesium ion: E261 (= E270), E273 (= E282)
O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
47% identity, 99% coverage: 4:394/396 of query aligns to 8:410/430 of O58056
2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
46% identity, 99% coverage: 2:394/396 of query aligns to 8:399/409 of 2dwcB
- active site: E265 (= E270), E277 (= E282), D284 (= D289), T285 (= T290), R372 (= R367)
- binding adenosine-5'-diphosphate: R120 (= R114), H159 (≠ V153), K161 (= K155), H190 (≠ F197), I191 (≠ V198), F193 (= F200), E196 (= E203), F267 (= F272), E277 (= E282)
3ax6A Crystal structure of n5-carboxyaminoimidazole ribonucleotide synthetase from thermotoga maritima
27% identity, 93% coverage: 27:395/396 of query aligns to 16:358/360 of 3ax6A
- active site: E231 (= E270), E244 (= E282), N251 (≠ D289), S252 (≠ T290), K330 (≠ R367)
- binding adenosine-5'-diphosphate: K101 (≠ R114), V136 (= V153), K138 (= K155), E164 (= E195), F166 (= F197), V167 (= V198), E172 (= E203), F233 (= F272), N243 (≠ S281)
3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 93% coverage: 22:388/396 of query aligns to 17:368/380 of 3v4sA
- binding adenosine-5'-diphosphate: R106 (= R114), K146 (= K155), Y152 (vs. gap), G154 (= G162), Q157 (= Q165), W183 (≠ F197), V184 (= V198), E189 (= E203), N215 (≠ G228), F256 (= F272), N266 (≠ S281), E267 (= E282)
- binding carbonate ion: R271 (= R286), H273 (= H288), N274 (≠ D289)
3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
27% identity, 93% coverage: 22:388/396 of query aligns to 18:369/377 of 3q2oB