SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_019557819.1 NCBI__GCF_000381085.1:WP_019557819.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4rjiC Acetolactate synthase from bacillus subtilis bound to thdp - crystal form i (see paper)
38% identity, 98% coverage: 2:536/547 of query aligns to 3:542/555 of 4rjiC

query
sites
4rjiC

K

R

A

G

A

A

K

E

L

L

F

V

V

V

K

D

C

C

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L

E

V

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E

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E

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E
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N

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Y

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M

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C

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G

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S

N

N

L

L

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A

T

T

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G

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L

A

L

D

T

A

A

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N

M

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N

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D

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P

L

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V

A

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Y

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N

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I

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-

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x
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A
x
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G
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E

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K

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W

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N

D
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C

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x
T

Y
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Y

G
x
D

L
x
M

I
x
V

E

A

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F

K

Q

Q

Q

Q

L

R

K

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Y

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N

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R

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T

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A

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D

D

F

F

K

G

N

N

P

I

D

D

F

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K

Y

Y

A

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E

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F

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G

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G

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R

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E

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L

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P

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Y
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Y

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S

Q

D

N

N

D

I

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N

L

L

T

A

A

S

binding magnesium ion: D438 (= D432), D465 (= D459), T467 (≠ Q461)
binding thiamine diphosphate: P24 (= P23), E48 (= E47), P74 (= P73), S387 (= S381), H388 (= H382), Q411 (≠ A405), G437 (= G431), D438 (= D432), G439 (≠ A433), G440 (= G434), T467 (≠ Q461), Y468 (= Y462), D469 (≠ G463), M470 (≠ L464), V471 (≠ I465), Y534 (= Y528)

4rjkF Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
38% identity, 98% coverage: 2:536/547 of query aligns to 2:541/552 of 4rjkF

query
sites
4rjkF

K

R

A

G

A

A

K

E

L

L

F

V

V

V

K

D

C

C

L

L

E

V

N

E

E

Q

N

G

V

V

E

T

F

H

I

V

F

F

G

G

I

I

P
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P

G

G

E
x
A

E
x
K

N

I

L

D

D

A

I

V

M

F

D

D

A

A

L

L

L

Q

D

D

S

K

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G

I

P

K

E

F

I

I

I

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V

T

A

R

R

H

H

E
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E

Q

Q

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N

A

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F

M

M

A

A

D

Q

V

A

Y

V

G

G

R

R

L

L

T

T

G

G

K

K

S

P

G

G

V

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C

V

L

L

S

V

T

T

L

S

G

G

P
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P

G

G

A

A

T

S

N

N

L

L

V

A

T

T

G

G

V

L

A

L

D

T

A

A

N

N

M

T

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E

N

G

A

D

P

P

L

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A

A

I

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N

A

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I

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A

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R

H

L

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K

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H

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Y

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G

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T

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I

H

H

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E

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L

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I

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N

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I

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K

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K

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M

M

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Y

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N

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G

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A
x
Q

G

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x
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G

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I

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A

A

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A

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x
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G

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E

E

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A

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N

K

A

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W

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x
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G
x
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L
x
M

I
x
V

E

A

W

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K

Q

Q

Q

Q

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R

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F

Y

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N

R

R

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T

A

S

Y

A

I

V

D

D

F

F

K

G

N

N

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I

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D

F

I

V

V

Q

K

Y

Y

A

A

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F

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L

A

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I

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D

N

N

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L

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A

A

S

binding magnesium ion: D437 (= D432), D464 (= D459), T466 (≠ Q461)
binding pyruvic acid: A25 (≠ E25), K26 (≠ E26)
binding thiamine diphosphate: P23 (= P23), E47 (= E47), P73 (= P73), G385 (= G380), S386 (= S381), H387 (= H382), Q410 (≠ A405), L412 (≠ M407), G436 (= G431), D437 (= D432), G438 (≠ A433), G439 (= G434), T466 (≠ Q461), Y467 (= Y462), D468 (≠ G463), M469 (≠ L464), V470 (≠ I465), Y533 (= Y528)

4rjkG Acetolactate synthase from bacillus subtilis bound to lthdp - crystal form ii (see paper)
38% identity, 98% coverage: 2:536/547 of query aligns to 2:541/553 of 4rjkG

query
sites
4rjkG

K

R

A

G

A

A

K

E

L

L

F

V

V

V

K

D

C

C

L

L

E

V

N

E

E

Q

N

G

V

V

E

T

F

H

I

V

F

F

G

G

I

I

P

P

G

G

E

A

E

K

N

I

L

D

D

A

I

V

M

F

D

D

A

A

L

L

L

Q

D

D

S

K

D

G

I

P

K

E

F

I

I

I

T

V

T

A

R

R

H

H

E
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E

Q

Q

G

N

A

A

Y

F

M

M

A

A

D

Q

V

A

Y

V

G

G

R

R

L

L

T

T

G

G

K

K

S

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G

G

V

V

C

V

L

L

S

V

T

T

L

S

G

G

P

P

G

G

A

A

T

S

N

N

L

L

V

A

T

T

G

G

V

L

A

L

D

T

A

A

N

N

M

T

D

E

N

G

A

D

P

P

L

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V

V

A

A

I

L

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A

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G

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N

A

V

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I

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R

T

A

R

D

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R

H

L

K

K

E

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H

H

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D

L

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A

M

L

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F

K

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P

I

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K

K

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Y

A

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D

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E

K

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P

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E

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A

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K

N

A

A

F

F

K

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L

I

A

A

E

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A

A

E

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K

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P

A

G

G

A

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A

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I

V

V

V

E

N

M

E

E

V

T

T

N

N

E

T

A

K

P

N

L

V

-

R

P

A

V

V

S

A

V

A

N

P

R

K

L

L

T

G

F

P

A

A

D

A

N

D

G

D

L

A

L

I

K

S

D

A

A

A

A

I

V

A

L

K

I

I

E

Q

K

T

A

A

K

K

N

L

P

P

L

V

I

V

L

L

V

V

G

G

N

M

G

K

A

G

V

G

R

R

S

P

R

E

A

A

S

I

D

K

H

A

I

V

L

R

A

K

F

L

L

L

K

K

T

K

H

V

K

Q

I

L

P

P

V

F

V

V

N

E

T

T

F

Y

M

Q

A

A

K

A

G

G

I

T

I

L

P

S

F

R

S

D

H

L

E

E

-

D

M

Q

A

Y

M

F

G

G

T

R

A

I

G

G

L

L

Q

F

K

R

G

N

D

Q

Y

P

E

G

N

D

G

L

F

L

A

E

K

Q

A

A

D

D

L

V

V

V

I

L

C

T

V

I

G

G

F

Y

D

D

M

P

V

I

E

E

Y

Y

H

D

P

P

H

K

L

F

W

W

N

N

P

I

T

N

R

G

K

D

H

R

T

T

I

I

H

H

I

L

D

D

T

E

K

I

S

A

E

D

V

I

D

D

N

H

S

A

Y

Y

I

Q

P

P

E

D

I

L

E

E

L

L

I

I

G

G

N

D

I

I

G

P

K

S

N

T

L

I

A

-

G

-

L

-

G

-

E

N

M

H

I

I

E

E

K

H

P

D

I

A

K

V

S

K

T

V

Q

E

I

F

D

A

F

E

R

R

L

E

R

Q

K

K

A

I

M

L

V

S

D

D

E

L

M

K

N

Q

R

Y

C

M

H

H

K

E

S

G

D

E

A

Q

W

V

P

P

M

A

-

D

-

W

-

K

-

S

-

D

-

R

-

A

L

H

P

P

Q

L

K

E

I

I

I

V

W

K

D

E

L

L

R

R

T

N

A

A

M

V

K

D

S

D

T

H

D

V

I

T

A

V

V

T

S

C

D

D

V
x
I

G
|
G

S
|
S

H
|
H

K

A

M

I

W

W

M

M

A

S

R

R

M

Y

F

F

R

R

C

S

D

Y

L

E

P

P

N

L

T

T

C

L

I

M

I

I

S

S

N

N

G

G

F

M

A
x
Q

G

T

M
x
L

G

G

I

V

A

A

V

L

P

P

G

W

A

A

I

I

A

G

A

A

K

S

L

L

A

V

Y

K

P

P

E

G

K

E

G

K

I

V

V

V

A

S

V

V

T

S

G
|
G

D
|
D

A
x
G

G
|
G

F

F

M

L

M

F

N

S

S

A

Q

M

E

E

I

L

E

E

T

T

A

A

L

V

R

R

C

L

N

K

A

A

P

P

I

I

V

V

I

H

L

I

V

V

W

W

N

N

D
|
D

C

S

Q
x
T

Y
|
Y

G
x
D

L
x
M

I
x
V

E

A

W

F

K

Q

Q

Q

Q

L

R

K

F

Y

G

N

R

R

S

T

A

S

Y

A

I

V

D

D

F

F

K

G

N

N

P

I

D

D

F

I

V

V

Q

K

Y

Y

A

A

H

E

S

S

F

F

G

G

A

A

T

T

G

G

V

L

R

R

I

V

E

E

S

S

A

P

D

D

Q

Q

L

L

L

A

P

D

A

V

L

L

Q

R

A

Q

A

G

L

M

E

N

N

A

P

E

T

G

V

P

T

V

I

I

D

D

C

V

P

P

V

V

D

D

Y
|
Y

S

S

Q

D

N

N

D

I

R

N

L

L

T

A

A

S

binding magnesium ion: D437 (= D432), D464 (= D459), T466 (≠ Q461)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: E47 (= E47), Q110 (= Q110)
binding thiamine diphosphate: I384 (≠ V379), G385 (= G380), S386 (= S381), H387 (= H382), Q410 (≠ A405), L412 (≠ M407), G436 (= G431), D437 (= D432), G438 (≠ A433), G439 (= G434), T466 (≠ Q461), Y467 (= Y462), D468 (≠ G463), M469 (≠ L464), V470 (≠ I465), Y533 (= Y528)

1ozgA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactor and with an unusual intermediate (see paper)
37% identity, 98% coverage: 4:538/547 of query aligns to 9:548/549 of 1ozgA

query
sites
1ozgA

A

A

K

D

L

L

F

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

N

G

V

V

E

R

F

Q

I

V

F

F

G

G

I
|
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

D

K

I

V

M

F

D

D

A

S

L

L

D

D

S

S

D

S

I

I

K

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

Y

F

M

M

A

A

D

A

V

A

Y

V

G

G

R

R

L

I

T

T

G

G

K

K

S

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

V

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

M

S

D

E

N

G

A

D

P

P

L

V

V

V

A

A

I

L

S

G

G

G

Q

A

A

V

A

K

T

R

T

A

R

D

L

K

H

A

K

K

E

Q

S

V

H
|
H

Q
|
Q

V
x
S

V

M

D

D

L

T

V

V

S

A

M

M

F

F

K

S

P

P

I

V

T

T

K

K

Y

Y

A

A

T

I

Q

E

V

V

L

T

A

A

P

P

E

D

T

A

I

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

E

E

A

Q

E

G

K

R

P

P

G

G

A

S

T

A

F

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

V

V

E

D

M

G

E

P

T

V

N

S

E

G

A

K

P

V

L

L

P

P

V

A

S

S

-

G

V

A

N

P

R

Q

L

M

T

G

F

A

A

A

D

P

N

D

G

D

L

A

L

I

K

D

D

Q

A

V

A

A

V

K

L

L

I

I

E

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

A

A

V

S

R

Q

S

P

R

E

A

N

S

S

D

K

H

A

I

L

L

R

A

R

F

L

L

L

K

E

T

T

H

S

K

H

I

I

P

P

V

V

V

T

N

S

T

T

F

Y

M

Q

A

A

K

A

G

G

I

A

I

V

-

N

-

Q

-

D

P

N

F

F

S

S

H

R

E

-

M

-

A

F

M

A

G
|
G

T
x
R

A

V

G

G

L
|
L

Q

F

K

N

G

N

D
x
Q

Y

A

E

G

N

D

G

R

G

L

F

L

A

Q

K

L

A

A

D

D

L

L

V

V

I

I

C

C

V

I

G

G

F

Y

D

S

M

P

V

V

E

E

Y

Y

H
x
E

P

P

H

A

L

M

W

W

N

N

P

-

T

S

R

G

K

N

H

A

T

T

I

L

I

V

H

H

I

I

D

D

T

V

K

L

K

P

S

A

E

Y

V

E

D

E

N

R

S

N

Y

Y

I

T

P

P

E

D

I

V

E

E

L

L

I

V

G

G

N

D

I

I

G

A

K

G

N

T

L

L

A

N

G

K

L

L

G

A

E

Q

M

N

I

I

E

D

K

H

P

R

I

L

-

V

-

L

-

S

-

P

K

Q

S

A

T

A

Q

E

I

I

-

L

-

R

D

D

F
x
R

R

Q

L

H

R

Q

K

R

A

E

M

L

V

L

D

D

E

R

M

-

N

-

R

R

C

G

H

A

K

Q

S

L

D

N

A

Q

W

F

P

A

M

L

L

H

P

P

Q

L

K

R

I

I

I

V

W

R

D

A

L

M

R

Q

T

D

A

I

M

V

K

N

S

S

T

D

D

V

I

T

A

L

V

T

S

V

D

D

V
x
M

G
|
G

S
|
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R
|
R

M

Y

F

L

R
x
Y

C

T

D

F

L

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

G

T

M
|
M

G

G

I

V

A

A

V

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

A

V

Y

N

P

P

E

E

K

R

G

K

I

V

V

V

A

S

V

V

T

S

G
|
G

D
|
D

A
x
G

G

G

F

F

M

L

M

Q

N

S

S

S

Q

M

E

E

I

L

E

E

T

T

A

A

L

V

R

R

C

L

N

K

A

A

P

N

I

V

V

L

I

H

L

L

V

I

W

W

N

V

D
|
D

C

N

Q
x
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

R

K

F

Y

G

Q

R

R

S

L

A

S

Y

G

I

V

D

E

F

F

K

G

N

P

P

M

D

D

F

F

V

K

Q

A

Y

Y

A

A

H

E

S

S

F

F

G

G

A

A

T

K

G

G

V

F

R

A

I

V

E

E

S

S

A

A

D

E

Q

A

L

L

L

E

P

P

A

T

L

L

Q

R

A

A

L

M

E

D

N

V

P

D

T

G

V

P

T

A

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

S

R

Q

D

N

N

D

P

R

L

L

L

T

M

A

G

L

Q

L

L

active site: I27 (= I22), G29 (= G24), A30 (≠ E25), K31 (≠ E26), I32 (≠ N27), E52 (= E47), T75 (= T70), H114 (= H109), Q115 (= Q110), S116 (≠ V111), Q164 (≠ E159), L257 (= L250), E284 (≠ H277), M389 (≠ V379), Q415 (≠ A405), M417 (= M407), D442 (= D432), D469 (= D459), G471 (≠ Q461), Y472 (= Y462), M474 (≠ L464), V475 (≠ I465), Q478 (≠ K468), Y538 (= Y528)
binding 2-hydroxyethyl dihydrothiachrome diphosphate: M389 (≠ V379), G390 (= G380), S391 (= S381), F392 (≠ H382), Q415 (≠ A405), M417 (= M407), G441 (= G431), D442 (= D432), G443 (≠ A433), D469 (= D459), G471 (≠ Q461), Y472 (= Y462), N473 (≠ G463), M474 (≠ L464), V475 (≠ I465), Y538 (= Y528)
binding magnesium ion: D442 (= D432), D469 (= D459), G471 (≠ Q461)
binding phosphate ion: G253 (= G246), R254 (≠ T247), Q261 (≠ D254), R347 (≠ F335), R398 (= R388), Y401 (≠ R391)

5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
37% identity, 98% coverage: 4:538/547 of query aligns to 8:545/548 of 5d6rB

query
sites
5d6rB

A

A

K

D

L

L

F

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

N

G

V

V

E

R

F

Q

I

V

F

F

G

G

I
|
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

D

K

I

V

M

F

D

D

A

S

L

L

D

D

S

S

D

S

I

I

K

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

Y

F

M

M

A

A

D

A

V

A

Y

V

G

G

R
|
R

L

I

T

T

G

G

K

K

S

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

V

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

M

S

D

E

N

G

A

D

P

P

L

V

V

V

A

A

I

L

S

G

G

G

Q

A

A

V

A

K

T

R

T

A

R

D

L

K

H

A

K

K

E

Q

S

V

H
|
H

Q
|
Q

V
x
S

V

M

D

D

L

T

V

V

S

A

M

M

F

F

K

S

P

P

I

V

T

T

K

K

Y

Y

A

A

T

I

Q

E

V

V

L

T

A

A

P

P

E

D

T

A

I

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

E

E

A

Q

E

G

K

R

P

P

G

G

A

S

T

A

F

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

V

V

E

D

M

G

E

P

T

V

N

S

E

G

A

K

P

V

L

L

P

P

V

A

S

S

V

-

N

P

R

Q

L

M

T

G

F

A

A

A

D

P

N

D

G

D

L

A

L

I

K

D

D

Q

A

V

A

A

V

K

L

L

I

I

E

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

A

A

V

S

R
x
Q

S

P

R

E

A

N

S

S

D

K

H

A

I

L

L

R

A

R

F

L

L

L

K

E

T

T

H

S

K

H

I

I

P

P

V

V

V

T

N

S

T

T

F

Y

M

Q

A

A

K

A

G

G

I

A

I

V

-

N

-

Q

-

D

P

N

F

F

S

S

H

R

E

-

M

-

A

F

M

A

G

G

T

R

A

V

G

G

L
|
L

Q

F

K

N

G

N

D

Q

Y

A

E

G

N

D

G

R

G

L

F

L

A

Q

K

L

A

A

D

D

L

L

V

V

I

I

C

C

V

I

G

G

F

Y

D

S

M

P

V

V

E

E

Y

Y

H
x
E

P

P

H

A

L

M

W

W

N

N

P

-

T

S

R

G

K

N

H

A

T

T

I

L

I

V

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active site: I26 (= I22), G28 (= G24), A29 (≠ E25), K30 (≠ E26), I31 (≠ N27), E51 (= E47), T74 (= T70), H113 (= H109), Q114 (= Q110), S115 (≠ V111), Q163 (≠ E159), L254 (= L250), E281 (≠ H277), M386 (≠ V379), Q412 (≠ A405), M414 (= M407), D439 (= D432), D466 (= D459), G468 (≠ Q461), Y469 (= Y462), M471 (≠ L464), V472 (≠ I465), Q475 (≠ K468), Y535 (= Y528)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (≠ V379), G387 (= G380), S388 (= S381), Q412 (≠ A405), M414 (= M407), D439 (= D432), G440 (≠ A433), G468 (≠ Q461), Y469 (= Y462), N470 (≠ G463), M471 (≠ L464), Y535 (= Y528)
binding magnesium ion: R63 (= R59), Q212 (≠ R209), D439 (= D432), D466 (= D459), G468 (≠ Q461)

5dx6B Acetolactate synthase from klebsiella pneumoniae soaked with beta- fluoropyruvate
37% identity, 98% coverage: 4:538/547 of query aligns to 20:551/557 of 5dx6B

query
sites
5dx6B

A

A

K

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active site: I38 (= I22), G40 (= G24), A41 (≠ E25), K42 (≠ E26), I43 (≠ N27), E63 (= E47), T86 (= T70), H125 (= H109), Q126 (= Q110), S127 (≠ V111), Q175 (≠ E159), L268 (= L250), E295 (≠ H277), M392 (≠ V379), Q418 (≠ A405), M420 (= M407), D445 (= D432), D472 (= D459), G474 (≠ Q461), Y475 (= Y462), M477 (≠ L464), V478 (≠ I465), Q481 (≠ K468), Y541 (= Y528)
binding 3-fluoro-2-oxopropanoic acid: G264 (= G246), R265 (≠ T247), Q272 (≠ D254), A400 (= A387), R401 (= R388), Y404 (≠ R391)
binding magnesium ion: S135 (≠ K119), T138 (= T122), D445 (= D432), D472 (= D459), G474 (≠ Q461)
binding thiamine diphosphate: G393 (= G380), S394 (= S381), F395 (≠ H382), Q418 (≠ A405), M420 (= M407), G444 (= G431), D445 (= D432), G446 (≠ A433), D472 (= D459), G474 (≠ Q461), Y475 (= Y462), N476 (≠ G463), M477 (≠ L464), V478 (≠ I465), Y541 (= Y528)

1ozfA The crystal structure of klebsiella pneumoniae acetolactate synthase with enzyme-bound cofactors (see paper)
37% identity, 98% coverage: 4:538/547 of query aligns to 8:544/545 of 1ozfA

query
sites
1ozfA

A

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R

M

Y

F
x
L

R
x
Y

C

T

D

F

L

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

G

T

M
|
M

G

G

I

V

A

A

V

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

A

V

Y

N

P

P

E

E

K

R

G

K

I

V

V

V

A

S

V

V

T

S

G
|
G

D
|
D

A
x
G

G

G

F

F

M

L

M

Q

N

S

S

S

Q

M

E

E

I

L

E

E

T

T

A

A

L

V

R

R

C

L

N

K

A

A

P

N

I

V

V

L

I

H

L

L

V

I

W

W

N

V

D
|
D

C

N

Q
x
G

Y
|
Y

G
x
N

L
x
M

I
x
V

E

A

W

I

K
x
Q

Q

E

R

K

F

Y

G

Q

R

R

S

L

A

S

Y

G

I

V

D

E

F

F

K

G

N

P

P

M

D

D

F

F

V

K

Q

A

Y

Y

A

A

H

E

S

S

F

F

G

G

A

A

T

K

G

G

V

F

R

A

I

V

E

E

S

S

A

A

D

E

Q

A

L

L

L

E

P

P

A

T

L

L

Q

R

A

A

L

M

E

D

N

V

P

D

T

G

V

P

T

A

I

V

D

A

C

I

P

P

V

V

D

D

Y
|
Y

S

R

Q

D

N

N

D

P

R

L

L

L

T

M

A

G

L

Q

L

L

active site: I26 (= I22), G28 (= G24), A29 (≠ E25), K30 (≠ E26), I31 (≠ N27), E51 (= E47), T74 (= T70), H113 (= H109), Q114 (= Q110), S115 (≠ V111), Q163 (≠ E159), L253 (= L250), E280 (≠ H277), M385 (≠ V379), Q411 (≠ A405), M413 (= M407), D438 (= D432), D465 (= D459), G467 (≠ Q461), Y468 (= Y462), M470 (≠ L464), V471 (≠ I465), Q474 (≠ K468), Y534 (= Y528)
binding magnesium ion: D438 (= D432), D465 (= D459), G467 (≠ Q461)
binding phosphate ion: G249 (= G246), R250 (≠ T247), Q257 (≠ D254), R343 (≠ F335), R394 (= R388), L396 (≠ F390), Y397 (≠ R391)
binding thiamine diphosphate: G386 (= G380), S387 (= S381), F388 (≠ H382), Q411 (≠ A405), M413 (= M407), G437 (= G431), D438 (= D432), G439 (≠ A433), D465 (= D459), G467 (≠ Q461), Y468 (= Y462), N469 (≠ G463), M470 (≠ L464), V471 (≠ I465), Y534 (= Y528)

5wdgA Acetolactate synthase from klebsiella pneumoniae in complex with a reaction intermediate
37% identity, 98% coverage: 4:538/547 of query aligns to 9:537/538 of 5wdgA

query
sites
5wdgA

A

A

K

D

L

L

F

V

V

V

K

S

C

Q

L

L

E

E

N

A

E

Q

N

G

V

V

E

R

F

Q

I

V

F

F

G

G

I
|
I

P

P

G
|
G

E
x
A

E
x
K

N
x
I

L

D

D

K

I

V

M

F

D

D

A

S

L

L

D

D

S

S

D

S

I

I

K

R

F

I

I

I

T

P

T

V

R

R

H

H

E
|
E

Q

A

G

N

A

A

Y

F

M

M

A

A

D

A

V

A

Y

V

G

G

R
|
R

L

I

T

T

G

G

K

K

S

A

G

G

V

V

C

A

L

L

S

V

T
|
T

L

S

G

G

P

P

G

G

A

C

T

S

N

N

L

L

V

I

T

T

G

G

V

M

A

A

D

T

A

A

N

N

M

S

D

E

N
x
G

A

D

P

P

L

V

V

V

A

A

I

L

S

G

G

G

Q

-

A

-

A

A

T

V

T

K

R

R

L

A

H

D

K

K

E

-

S

-

H

-

Q

-

V

-

V

M

D

D

L

T

V

V

S

A

M

M

F

F

K
x
S

P

P

I

V

T
|
T

K

K

Y

Y

A

A

T

I

Q

E

V

V

L

T

A

A

P

P

E

D

T

A

I

L

P

A

E

E

V

V

R

S

K

N

A

A

F

F

K

R

L

A

A

A

E

E

A

Q

E

G

K
x
R

P

P

G

G

A

S

T

A

F

F

I

V

D

S

L

L

P

P

E
x
Q

N

D

I

V

V

V

E

D

M

G

E

P

T

V

N

S

E

G

A

K

P

V

L

L

P

P

V

A

S

-

V

-

N

-

R

Q

L

M

T

G

F

A

A

A

D

P

N

D

G

D

L

A

L

I

K

D

D

Q

A

V

A

A

V

K

L

L

I

I

E

A

K

Q

A

A

K

K

N

N

P

P

L

I

I

F

L

L

V

L

G

G

N

L

G

M

A

A

V

S

R
x
Q

S

P

R

E

A

N

S

S

D

K

H

A

I

L

L

R

A

R

F

L

L

L

K

E

T

T

H

S

K

H

I

I

P

P

V

V

V

T

N

S

T

T

F

Y

M

Q

A

A

K

A

G

G

I

A

I

V

P

N

-

Q

-

D

-

N

F

F

S

S

H

R

E

-

M

-

A

F

M

A

G

G

T

R

A

V

G

G

L
|
L

Q

F

K

N

G

N

D

Q

Y

A

E

G

N

D

G

R

G

L

F

L

A

Q

K

L

A

A

D

D

L

L

V

V

I

I

C

C

V

I

G

G

F

Y

D

S

M

P

V

V

E

E

Y

Y

H
x
E

P

P

H

A

L

M

W

W

N

N

P

-

T

S

R

G

K

N

H

A

T

T

I

L

I

V

H

H

I

I

D

D

T

V

K

L

K

P

S

A

E

Y

V

E

D

E

N

R

S

N

Y

Y

I

T

P

P

E

D

I

V

E

E

L

L

I

V

G

G

N

D

I

I

G

A

K

G

N

T

L

L

A

N

G

K

L

L

G

A

E

Q

M

N

I

I

E

D

K

H

P

R

I

L

-

V

-

L

-

S

-

P

K

Q

S

A

T

A

Q

E

I

I

-

L

-

R

D

D

F

R

R

Q

L

H

R

Q

K

R

A

E

M

L

V

L

D

D

E

R

M

-

N

-

R

R

C

G

H

A

K

Q

S

L

D

N

A

Q

W

F

P

A

M

L

L

H

P

P

Q

L

K

R

I

I

I

V

W

R

D

A

L

M

R

Q

T

D

A

I

M

V

K

N

S

S

T

D

D

V

I

T

A

L

V

T

S

V

D

D

V
x
M

G

G

S
|
S

H
x
F

K

H

M

I

W

W

M

I

A

A

R

R

M

Y

F

L

R

Y

C

S

D

F

L

R

P

A

N

R

T

Q

C

V

I

M

I

I

S

S

N

N

G

G

F

Q

A
x
Q

G

T

M
|
M

G

G

I

V

A

A

V

L

P

P

G

W

A

A

I

I

A

G

A

A

K

W

L

L

A

V

Y

N

P

P

E

E

K

R

G

K

I

V

V

V

A

S

V

V

T

S

G
|
G