SitesBLAST

O00116 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Aging-associated gene 5 protein; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Homo sapiens (Human) (see 2 papers)
26% identity, 51% coverage: 39:227/370 of query aligns to 232:426/658 of O00116

query
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O00116

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R419 (≠ T220) Important for enzyme activity; to H: in RCDP3; loss of enzyme activity; dbSNP:rs121434411

Sites not aligning to the query:

182 R → Q: in RCDP3; severely reduced protein levels
471 E → K: in RCDP3; severely reduced protein levels
568 T → M: in RCDP3; does not affect protein levels; dbSNP:rs387907214

5ae3A Ether lipid-generating enzyme agps in complex with antimycin a (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 138:295/539 of 5ae3A

query
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5ae3A

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binding flavin-adenine dinucleotide: P140 (≠ I41), I141 (≠ C42), G142 (= G43), G143 (= G44), G144 (= G45), T145 (≠ S46), S146 (≠ K47), V147 (≠ R48), L151 (≠ R52), P208 (= P103), D209 (≠ P104), S210 (≠ H105), S214 (≠ A110), T215 (= T111), G218 (= G114), W219 (≠ T115), S221 (≠ A117), T222 (≠ C118), A224 (≠ L120), S225 (= S121), E274 (≠ Q171), V279 (= V176), I280 (≠ L177)

Sites not aligning to the query:

5adzC Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 152:309/557 of 5adzC

query
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5adzC

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binding flavin-adenine dinucleotide: P154 (≠ I41), I155 (≠ C42), G156 (= G43), G157 (= G44), G158 (= G45), T159 (≠ S46), S160 (≠ K47), P222 (= P103), D223 (≠ P104), S224 (≠ H105), S228 (≠ A110), T229 (= T111), G232 (= G114), W233 (≠ T115), S235 (≠ A117), T236 (≠ C118), A238 (≠ L120), S239 (= S121), E288 (≠ Q171), G289 (= G172), I294 (≠ L177)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D223 (≠ P104)

Sites not aligning to the query:

6gouA Development of alkyl glycerone phosphate synthase inhibitors: complex with inhibitor 2i (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 152:309/554 of 6gouA

query
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binding (3~{S})-3-[2,6-bis(fluoranyl)phenyl]-~{N}-[(2-oxidanylidene-1,3-dihydrobenzimidazol-5-yl)methyl]butanamide: D223 (≠ P104)
binding flavin-adenine dinucleotide: P154 (≠ I41), I155 (≠ C42), G156 (= G43), G157 (= G44), G158 (= G45), T159 (≠ S46), S160 (≠ K47), V161 (≠ R48), G164 (= G51), P222 (= P103), D223 (≠ P104), S224 (≠ H105), S228 (≠ A110), T229 (= T111), G232 (= G114), W233 (≠ T115), S235 (≠ A117), T236 (≠ C118), A238 (≠ L120), S239 (= S121), E288 (≠ Q171), V293 (= V176), I294 (≠ L177)

Sites not aligning to the query:

4bc9B Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
25% identity, 51% coverage: 39:227/370 of query aligns to 138:332/542 of 4bc9B

query
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4bc9B

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binding propanenitrile: D209 (≠ P104)
binding flavin-adenine dinucleotide: P140 (≠ I41), G142 (= G43), G143 (= G44), G144 (= G45), T145 (≠ S46), S146 (≠ K47), V147 (≠ R48), G150 (= G51), L151 (≠ R52), P208 (= P103), D209 (≠ P104), S210 (≠ H105), S214 (≠ A110), T215 (= T111), G218 (= G114), W219 (≠ T115), S221 (≠ A117), T222 (≠ C118), A224 (≠ L120), S225 (= S121), E274 (≠ Q171), G275 (= G172), I280 (≠ L177)

Sites not aligning to the query:

5ae1B Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 138:295/539 of 5ae1B

query
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5ae1B

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binding flavin-adenine dinucleotide: P140 (≠ I41), I141 (≠ C42), G142 (= G43), G143 (= G44), G144 (= G45), T145 (≠ S46), S146 (≠ K47), G150 (= G51), L151 (≠ R52), P208 (= P103), D209 (≠ P104), S210 (≠ H105), S214 (≠ A110), T215 (= T111), G218 (= G114), W219 (≠ T115), S221 (≠ A117), T222 (≠ C118), A224 (≠ L120), S225 (= S121), E274 (≠ Q171), V279 (= V176), I280 (≠ L177)

Sites not aligning to the query:

5ae2A Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 143:300/561 of 5ae2A

query
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5ae2A

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-

T

-

R

-

T

-

I

T

S

L

L

S

D

L

T

S

S

D

Q

Y

M

Q

N

G

R

I

I

V

L

S

W

Y

V

E

D

P

E

S

N

E

N

L

L

V

T

I

A

T

H

A

V

R

E

A

A

G

G

T

I

P

T

L

G

Q

Q

V

E

I

L

E

E

T

R

V

Q

L

L

A

K

E

E

Q

S

G

G

Q

Y

M

C

L

T

G

G

F

H

E

E

P
|
P

P
x
D

H
x
S

Y

-

A

L

D

E

N

F

A
x
S

T
|
T

L

V

G

G

G
|
G

T
x
W

I

I

A
x
S

C
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

P

N

Y

I

S

Y

G

G

A

N

A

I

R

E

D

D

F

L

V

V

L

V

G

H

V

M

T

K

M

V

I

V

N

T

G

P

K

R

G

G

E

-

I

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

M

I

S

M

G

G

A

S

Q
x
E

G

G

T

T

L

L

G

G

V
|
V

L
x
I

L

T

E

E

I

A

S

T

L

I

K

K

V

I

I

R

P

P

M

T

P

P

A

E

H

Y

E

Q

E

K

binding flavin-adenine dinucleotide: P145 (≠ I41), I146 (≠ C42), G147 (= G43), G148 (= G44), G149 (= G45), T150 (≠ S46), S151 (≠ K47), G155 (= G51), L156 (≠ R52), P213 (= P103), D214 (≠ P104), S215 (≠ H105), S219 (≠ A110), T220 (= T111), G223 (= G114), W224 (≠ T115), S226 (≠ A117), T227 (≠ C118), A229 (≠ L120), S230 (= S121), E279 (≠ Q171), V284 (= V176), I285 (≠ L177)

Sites not aligning to the query:

4bc7B Mammalian alkyldihydroxyacetonephosphate synthase: arg419his mutant (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 138:295/543 of 4bc7B

query
sites
4bc7B

L

I

V

I

I
x
P

C

I

G
|
G

S
x
T

K
x
S

R
x
V

F

S

Y

Y

G
|
G

R
x
L

P

M

L

C

E

P

G

A

E

D

-

E

-

T

-

R

-

T

-

I

T

S

L

L

S

D

L

T

S

S

D

Q

Y

M

Q

N

G

R

I

I

V

L

S

W

Y

V

E

D

P

E

S

N

E

N

L

L

V

T

I

A

T

H

A

V

R

E

A

A

G

G

T

I

P

T

L

G

Q

Q

V

E

I

L

E

E

T

R

V

Q

L

L

A

K

E

E

Q

S

G

G

Q

Y

M

C

L

T

G

G

F

H

E

E

P
|
P

P
x
D

H
x
S

Y

-

A

L

D

E

N

F

A
x
S

T
|
T

L

V

G

G

G
|
G

T

W

I

I

A
x
S

C
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

P

N

Y

I

S

Y

G

G

A

N

A

I

R

E

D

D

F

L

V

V

L

V

G

H

V

M

T

K

M

V

I

V

N

T

G

P

K

R

G

G

E

-

I

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

M

I

S

M

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V

V

L
x
I

L

T

E

E

I

A

S

T

L

I

K

K

V

I

I

R

P

P

M

T

P

P

A

E

H

Y

E

Q

E

K

binding flavin-adenine dinucleotide: P140 (≠ I41), G142 (= G43), G143 (= G44), G144 (= G45), T145 (≠ S46), S146 (≠ K47), V147 (≠ R48), G150 (= G51), L151 (≠ R52), P208 (= P103), D209 (≠ P104), S210 (≠ H105), S214 (≠ A110), T215 (= T111), G218 (= G114), S221 (≠ A117), T222 (≠ C118), A224 (≠ L120), S225 (= S121), E274 (≠ Q171), G275 (= G172), I280 (≠ L177)

Sites not aligning to the query:

P97275 Alkyldihydroxyacetonephosphate synthase, peroxisomal; Alkyl-DHAP synthase; Alkylglycerone-phosphate synthase; EC 2.5.1.26 from Cavia porcellus (Guinea pig) (see 4 papers)
26% identity, 42% coverage: 39:192/370 of query aligns to 232:389/658 of P97275

query
sites
P97275

L

I

V

I

I
x
P

C
x
I

G
|
G

S
x
T

K
x
S

R

V

F

S

Y

Y

G

G

R

L

P

M

L

C

E

P

G

A

E

D

-

E

-

T

-

R

-

T

-

I

T

S

L

L

S

D

L

T

S

S

D

Q

Y

M

Q

N

G

R

I

I

V

L

S

W

Y

V

E

D

P

E

S

N

E

N

L

L

V

T

I

A

T

H

A

V

R

E

A

A

G

G

T

I

P

T

L

G

Q

Q

V

E

I

L

E

E

T

R

V

Q

L

L

A

K

E

E

Q

S

G

G

Q

Y

M

C

L

T

G

G

F
x
H

E

E

P

P

P
x
D

H
x
S

Y
x
L

A

-

D
x
E

N
x
F

A
x
S

T
|
T

L

V

G

G

T

W

I

I

A

S

C
x
T

G
x
R

L
x
A

S
|
S

G

G

P

M

R

K

P

N

Y

I

S

Y

G

G

A

N

A

I

R

E

D

D

F

L

V

V

L

V

G

H

V

M

T

K

M

V

I

V

N

T

G

P

K

R

G

G

E

-

I

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

M

I

S

M

G

G

A
x
S

Q
x
E

G
|
G

T
|
T

L
|
L

G
|
G

V
|
V

L
x
I

L

T

E

E

I

A

S

T

L

I

K

K

V

I

I

R

P

P

M

T

P

P

A

E

H

Y

E

Q

E

K

234:240 (vs. 41:47, 43% identical) binding FAD
H300 (≠ F101) mutation to A: Loss of activity.
303:309 (vs. 104:111, 13% identical) binding FAD
T309 (= T111) mutation to I: Impaired FAD binding and protein stability. Loss of activity.
TRAS 316:319 (≠ CGLS 118:121) binding FAD
S367 (≠ A170) mutation to A: Strongly reduced activity.
368:374 (vs. 171:177, 71% identical) binding FAD

Sites not aligning to the query:

1:58 modified: transit peptide, Peroxisome
419 Important for enzyme activity; R→H: Loss of activity.; R→K: Strongly reduced activity.
469 L→P: Impaired FAD binding and protein stability. Loss of activity.
515 binding substrate; R→L: Impaired FAD binding and protein stability. Loss of activity.
576 C→A: No effect on activity.
578 active site, Proton donor/acceptor; Y→F: Loss of activity.
615 H→A: Loss of activity.
615:617 Important for enzyme activity
616 H→A: Loss of activity.
617 H→A: Loss of activity.
654:658 Important for enzyme activity

4bc9A Mammalian alkyldihydroxyacetonephosphate synthase: wild-type, adduct with cyanoethyl (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 143:300/555 of 4bc9A

query
sites
4bc9A

L

I

V

I

I
x
P

C
x
I

G
|
G

S
x
T

K
x
S

R
x
V

F

S

Y

Y

G
|
G

R
x
L

P

M

L

C

E

P

G

A

E

D

-

E

-

T

-

R

-

T

-

I

T

S

L

L

S

D

L

T

S

S

D

Q

Y

M

Q

N

G

R

I

I

V

L

S

W

Y

V

E

D

P

E

S

N

E

N

L

L

V

T

I

A

T

H

A

V

R

E

A

A

G

G

T

I

P

T

L

G

Q

Q

V

E

I

L

E

E

T

R

V

Q

L

L

A

K

E

E

Q

S

G

G

Q

Y

M

C

L

T

G

G

F

H

E

E

P
|
P

P
x
D

H
x
S

Y

-

A

L

D

E

N

F

A
x
S

T
|
T

L

V

G

G

G
|
G

T
x
W

I

I

A
x
S

C
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

P

N

Y

I

S

Y

G

G

A

N

A

I

R

E

D

D

F

L

V

V

L

V

G

H

V

M

T

K

M

V

I

V

N

T

G

P

K

R

G

G

E

-

I

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

M

I

S

M

G

G

A

S

Q
x
E

G

G

T

T

L

L

G

G

V
|
V

L
x
I

L

T

E

E

I

A

S

T

L

I

K

K

V

I

I

R

P

P

M

T

P

P

A

E

H

Y

E

Q

E

K

binding propanenitrile: D214 (≠ P104)
binding flavin-adenine dinucleotide: P145 (≠ I41), I146 (≠ C42), G147 (= G43), G148 (= G44), G149 (= G45), T150 (≠ S46), S151 (≠ K47), V152 (≠ R48), G155 (= G51), L156 (≠ R52), P213 (= P103), D214 (≠ P104), S215 (≠ H105), S219 (≠ A110), T220 (= T111), G223 (= G114), W224 (≠ T115), S226 (≠ A117), T227 (≠ C118), A229 (≠ L120), S230 (= S121), E279 (≠ Q171), V284 (= V176), I285 (≠ L177)

Sites not aligning to the query:

5ae2B Ether lipid-generating enzyme agps in complex with inhibitor 1e (see paper)
26% identity, 42% coverage: 39:192/370 of query aligns to 138:295/542 of 5ae2B

query
sites
5ae2B

L

I

V

I

I
x
P

C

I

G
|
G

S
x
T

K
x
S

R

V

F

S

Y

Y

G
|
G

R
x
L

P

M

L

C

E

P

G

A

E

D

-

E

-

T

-

R

-

T

-

I

T

S

L

L

S

D

L

T

S

S

D

Q

Y

M

Q

N

G

R

I

I

V

L

S

W

Y

V

E

D

P

E

S

N

E

N

L

L

V

T

I

A

T

H

A

V

R

E

A

A

G

G

T

I

P

T

L

G

Q

Q

V

E

I

L

E

E

T

R

V

Q

L

L

A

K

E

E

Q

S

G

G

Q

Y

M

C

L

T

G

G

F

H

E

E

P
|
P

P
x
D

H
x
S

Y

-

A

L

D

E

N

F

A
x
S

T
|
T

L

V

G

G

G
|
G

T
x
W

I

I

A
x
S

C
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

P

N

Y

I

S

Y

G

G

A

N

A

I

R

E

D

D

F

L

V

V

L

V

G

H

V

M

T

K

M

V

I

V

N

T

G

P

K

R

G

G

E

-

I

V

L

I

R

E

F

K

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

M

I

S

M

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V
|
V

L
x
I

L

T

E

E

I

A

S

T

L

I

K

K

V

I

I

R

P

P

M

T

P

P

A

E

H

Y

E

Q

E

K

binding flavin-adenine dinucleotide: P140 (≠ I41), G142 (= G43), G143 (= G44), G144 (= G45), T145 (≠ S46), S146 (≠ K47), G150 (= G51), L151 (≠ R52), P208 (= P103), D209 (≠ P104), S210 (≠ H105), S214 (≠ A110), T215 (= T111), G218 (= G114), W219 (≠ T115), S221 (≠ A117), T222 (≠ C118), A224 (≠ L120), S225 (= S121), E274 (≠ Q171), G275 (= G172), V279 (= V176), I280 (≠ L177)

Sites not aligning to the query:

5ae1A Ether lipid-generating enzyme agps in complex with inhibitor zinc69435460 (see paper)
27% identity, 42% coverage: 39:192/370 of query aligns to 137:294/560 of 5ae1A

query
sites
5ae1A

L

I

V

I

I
x
P

C
x
I

G
|
G

S
x
T

K
x
S

R
x
V

F

S

Y

Y

G
|
G

R
x
L

P

M

L

C

E

P

G

A

E

D

-

E

-

T

-

R

-

T

-

I

T

S

L

L

S

D

L

T

S

S

D

Q

Y

M

Q

N

G

R

I

I

V

L

S

W

Y

V

E

D

P

E

S

N

E

N

L

L

V

T

I

A

T

H

A

V

R

E

A

A

G

G

T

I

P

T

L

G

Q

Q

V

E

I

L

E

E

T

R

V

Q

L

L

A

K

E

E

Q

S

G

G

Q

Y

M

C

L

T

G

G

F

H

E

E

P
|
P

P
x
D

H
x
S

Y

-

A

L

D

E

N

F

A
x
S

T
|
T

L

V

G
|
G

T
x
W

I

I

A
x
S

C
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

P

N

Y

I

S

Y

G

G

A

N

A

I

R

E

D

D

F

L

V

V

L

V

G

H

V

M

T

K

M

V

I

V

N

T

G

P

K

R

G

G

E

V

I

I

L

E

R

K

F

-

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

M

I

S

M

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V
|
V

L
x
I

L

T

E

E

I

A

S

T

L

I

K

K

V

I

I

R

P

P

M

T

P

P

A

E

H

Y

E

Q

E

K

binding flavin-adenine dinucleotide: P139 (≠ I41), I140 (≠ C42), G141 (= G43), G142 (= G44), G143 (= G45), T144 (≠ S46), S145 (≠ K47), V146 (≠ R48), G149 (= G51), L150 (≠ R52), P207 (= P103), D208 (≠ P104), S209 (≠ H105), S213 (≠ A110), T214 (= T111), G216 (= G113), G217 (= G114), W218 (≠ T115), S220 (≠ A117), T221 (≠ C118), A223 (≠ L120), S224 (= S121), E273 (≠ Q171), G274 (= G172), V278 (= V176), I279 (≠ L177)

Sites not aligning to the query:

5adzA Ether lipid-generating enzyme agps in complex with inhibitor 1a (see paper)
27% identity, 42% coverage: 39:192/370 of query aligns to 140:297/566 of 5adzA

query
sites
5adzA

L

I

V

I

I
x
P

C
x
I

G
|
G

S
x
T

K
x
S

R

V

F

S

Y

Y

G
|
G

R

L

P

M

L

C

E

P

G

A

E

D

-

E

-

T

-

R

-

T

-

I

T

S

L

L

S

D

L

T

S

S

D

Q

Y

M

Q

N

G

R

I

I

V

L

S

W

Y

V

E

D

P

E

S

N

E

N

L

L

V

T

I

A

T

H

A

V

R

E

A

A

G

G

T

I

P

T

L

G

Q

Q

V

E

I

L

E

E

T

R

V

Q

L

L

A

K

E

E

Q

S

G

G

Q

Y

M

C

L

T

G

G

F

H

E

E

P
|
P

P
x
D

H
x
S

Y

-

A

L

D

E

N

F

A
x
S

T
|
T

L

V

G

G

G
|
G

T
x
W

I

I

A
x
S

C
x
T

G

R

L
x
A

S
|
S

G

G

P

M

R

K

P

N

Y

I

S

Y

G

G

A

N

A

I

R

E

D

D

F

L

V

V

L

V

G

H

V

M

T

K

M

V

I

V

N

T

G

P

K

R

G

G

E

V

I

I

L

E

R

K

F

-

G

S

G

C

Q

Q

V

G

M

P

K

R

N

M

V

S

A

T

G

G

Y

P

D

D

V

I

S

H

R

H

L

F

M

I

S

M

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V
|
V

L
x
I

L

T

E

E

I

A

S

T

L

I

K

K

V

I

I

R

P

P

M

T

P

P

A

E

H

Y

E

Q

E

K

binding flavin-adenine dinucleotide: P142 (≠ I41), I143 (≠ C42), G144 (= G43), G145 (= G44), G146 (= G45), T147 (≠ S46), S148 (≠ K47), G152 (= G51), P210 (= P103), D211 (≠ P104), S212 (≠ H105), S216 (≠ A110), T217 (= T111), G220 (= G114), W221 (≠ T115), S223 (≠ A117), T224 (≠ C118), A226 (≠ L120), S227 (= S121), E276 (≠ Q171), G277 (= G172), V281 (= V176), I282 (≠ L177)
binding (3S)-3-(2-fluorophenyl)-N-((2-oxo-2,3-dihydro-1H-benzo[d]imidazol-5-yl)methyl)butanamide): D211 (≠ P104)

Sites not aligning to the query:

8jdsA Crystal structure of mldhd in complex with pyruvate (see paper)
26% identity, 40% coverage: 56:204/370 of query aligns to 85:231/456 of 8jdsA

query
sites
8jdsA

G

G

E

V

T

C

L

I

S

N

L
|
L

S

T

D

H

Y

M

Q

D

G

Q

I

I

V

T

S

E

Y

L

E

N

P

T

S

E

E

D

L

F

V

S

I

V

T

V

A

V

R

E

A

P

G

G

T

V

P

T

L

R

Q

K

V

A

I

L

E

N

T

T

V

H

L

L

A

R

E

D

Q

S

G

G

Q

L

M

W

L

F

G

P

F

V

E

D

P
|
P

P

-

H

-

Y

-

A
x
G

D
x
A

N

D

A

A

T

S

L

L

G

C

G
|
G

T
x
M

I

A

A
|
A

C
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

P

V

Y

R

S

Y

G

G

A

T

A

M

R

R

D

D

F

N

V

V

L

I

G

N

V

L

T

E

M

V

I

V

N

L

G

P

K

D

G

G

E

R

I

L

L

L

R

H

F

T

G

A

G

G

Q

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

S

V

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

L

T

E

S

I

T

S

T

L

L

K

R

V

L

I

H

P

P

M

A

P

P

A

-

H

-

E

E

E

A

T

T

R

V

V

A

L

A

S

T

C

C

S

A

Q

F

Q

P

Q

S

M

V

Q

Q

binding flavin-adenine dinucleotide: L90 (= L61), P132 (= P103), G133 (≠ A107), A134 (≠ D108), G140 (= G114), M141 (≠ T115), A143 (= A117), T144 (≠ C118), A146 (≠ L120), S147 (= S121), E200 (≠ Q171), G201 (= G172), I206 (≠ L177)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 323, 414, 415, 451
binding manganese (ii) ion: 370, 377, 414
binding pyruvic acid: 319, 370, 377, 415

8jdxA Crystal structure of mldhd in complex with 2-ketoisovaleric acid (see paper)
26% identity, 40% coverage: 56:204/370 of query aligns to 85:231/455 of 8jdxA

query
sites
8jdxA

G

G

E

V

T

C

L

I

S

N

L
|
L

S

T

D

H

Y

M

Q

D

G

Q

I

I

V

T

S

E

Y

L

E

N

P

T

S

E

E

D

L

F

V

S

I

V

T

V

A

V

R

E

A

P

G

G

T

V

P

T

L

R

Q

K

V

A

I

L

E

N

T

T

V

H

L

L

A

R

E

D

Q

S

G

G

Q

L

M

W

L

F

G

P

F

V

E

D

P
|
P

P

-

H

-

Y

-

A
x
G

D
x
A

N

D

A

A

T

S

L

L

G

C

G
|
G

T
x
M

I

A

A
|
A

C
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

P

V

Y

R

S

Y

G

G

A

T

A

M

R

R

D

D

F

N

V

V

L

I

G

N

V

L

T

E

M

V

I

V

N

L

G

P

K

D

G

G

E

R

I

L

L

L

R

H

F

T

G

A

G

G

Q

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

S

V

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

L

T

E

S

I

T

S

T

L

L

K

R

V

L

I

H

P

P

M

A

P

P

A

-

H

-

E

E

E

A

T

T

R

V

V

A

L

A

S

T

C

C

S

A

Q

F

Q

P

Q

S

M

V

Q

Q

binding flavin-adenine dinucleotide: L90 (= L61), P132 (= P103), G133 (≠ A107), A134 (≠ D108), G140 (= G114), M141 (≠ T115), A143 (= A117), T144 (≠ C118), A146 (≠ L120), S147 (= S121), E200 (≠ Q171), G201 (= G172), I206 (≠ L177)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 32, 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
binding 3-methyl-2-oxobutanoic acid: 318, 369, 376, 414
binding manganese (ii) ion: 369, 376, 413

8jduA Crystal structure of mldhd in complex with 2-ketovaleric acid (see paper)
26% identity, 40% coverage: 56:204/370 of query aligns to 85:231/455 of 8jduA

query
sites
8jduA

G

G

E

V

T

C

L

I

S

N

L
|
L

S

T

D

H

Y

M

Q

D

G

Q

I

I

V

T

S

E

Y

L

E

N

P

T

S

E

E

D

L

F

V

S

I

V

T

V

A

V

R

E

A

P

G

G

T

V

P

T

L

R

Q

K

V

A

I

L

E

N

T

T

V

H

L

L

A

R

E

D

Q

S

G

G

Q

L

M

W

L

F

G

P

F

V

E

D

P
|
P

P

-

H

-

Y

-

A
x
G

D
x
A

N

D

A

A

T

S

L

L

G

C

G
|
G

T
x
M

I

A

A
|
A

C
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

P

V

Y

R

S

Y

G

G

A

T

A

M

R

R

D

D

F

N

V

V

L

I

G

N

V

L

T

E

M

V

I

V

N

L

G

P

K

D

G

G

E

R

I

L

L

L

R

H

F

T

G

A

G

G

Q

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

S

V

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

L

T

E

S

I

T

S

T

L

L

K

R

V

L

I

H

P

P

M

A

P

P

A

-

H

-

E

E

E

A

T

T

R

V

V

A

L

A

S

T

C

C

S

A

Q

F

Q

P

Q

S

M

V

Q

Q

binding flavin-adenine dinucleotide: L90 (= L61), P132 (= P103), G133 (≠ A107), A134 (≠ D108), G140 (= G114), M141 (≠ T115), A143 (= A117), T144 (≠ C118), A146 (≠ L120), S147 (= S121), E200 (≠ Q171), G201 (= G172), I206 (≠ L177)

Sites not aligning to the query:

binding 2-oxopentanoic acid: 318, 322, 369, 376, 414
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 450
binding manganese (ii) ion: 369, 376, 413

8jdtA Crystal structure of mldhd in complex with 2-ketobutanoic acid (see paper)
26% identity, 40% coverage: 56:204/370 of query aligns to 85:231/455 of 8jdtA

query
sites
8jdtA

G

G

E

V

T

C

L

I

S

N

L
|
L

S

T

D

H

Y

M

Q

D

G

Q

I

I

V

T

S

E

Y

L

E

N

P

T

S

E

E

D

L

F

V

S

I

V

T

V

A

V

R

E

A

P

G

G

T

V

P

T

L

R

Q

K

V

A

I

L

E

N

T

T

V

H

L

L

A

R

E

D

Q

S

G

G

Q

L

M

W

L

F

G

P

F

V

E

D

P
|
P

P

-

H

-

Y

-

A
x
G

D
x
A

N

D

A

A

T

S

L

L

G

C

G
|
G

T
x
M

I

A

A
|
A

C
x
T

G

G

L
x
A

S
|
S

G

G

P

T

R

N

R

A

P

V

Y

R

S

Y

G

G

A

T

A

M

R

R

D

D

F

N

V

V

L

I

G

N

V

L

T

E

M

V

I

V

N

L

G

P

K

D

G

G

E

R

I

L

L

L

R

H

F

T

G

A

G

G

Q

R

-

G

-

R

-

H

V

Y

M

R

K

K

N

S

V

A

A

A

G

G

Y

Y

D

N

V

L

S

T

R

G

L

L

M

F

S

V

G

G

A

S

Q
x
E

G
|
G

T

T

L

L

G

G

V

I

L
x
I

L

T

E

S

I

T

S

T

L

L

K

R

V

L

I

H

P

P

M

A

P

P

A

-

H

-

E

E

E

A

T

T

R

V

V

A

L

A

S

T

C

C

S

A

Q

F

Q

P

Q

S

M

V

Q

Q

binding flavin-adenine dinucleotide: L90 (= L61), P132 (= P103), G133 (≠ A107), A134 (≠ D108), G140 (= G114), M141 (≠ T115), A143 (= A117), T144 (≠ C118), A146 (≠ L120), S147 (= S121), E200 (≠ Q171), G201 (= G172), I206 (≠ L177)

Sites not aligning to the query:

binding 2-ketobutyric acid: 318, 369, 376, 414
binding flavin-adenine dinucleotide: 68, 70, 71, 72, 73, 74, 78, 79, 322, 413, 414, 450
binding manganese (ii) ion: 369, 376, 413

Query Sequence

>WP_019621127.1 NCBI__GCF_000381785.1:WP_019621127.1
MQAVAEQVFPGALTQDQARDQAQSLANQVEQAFVNQQPLVICGGGSKRFYGRPLEGETLS
LSDYQGIVSYEPSELVITARAGTPLQVIETVLAEQGQMLGFEPPHYADNATLGGTIACGL
SGPRRPYSGAARDFVLGVTMINGKGEILRFGGQVMKNVAGYDVSRLMSGAQGTLGVLLEI
SLKVIPMPAHEETRVLSCSQQQMQTLLCDLGRQPLPISATLFHNDQLYIRLSGATAGVHS
AAQIIGGERLSTDQFWLQAKEQQHPFFHPAQPCWRLSLPPAAAAVPDALARHQLIEWGGA
QRWLYSEADPCQIIQWAEANGGHATAVDRSEPGHTLFHPLSNALLPLTQRIKQSFDPAGI
LNPGRLYTEL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory