SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_019623149.1 NCBI__GCF_000381785.1:WP_019623149.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P07821 Iron(3+)-hydroxamate import ATP-binding protein FhuC; Ferric hydroxamate uptake protein C; Ferrichrome transport ATP-binding protein FhuC; Iron(III)-hydroxamate import ATP-binding protein FhuC; EC 7.2.2.16 from Escherichia coli (strain K12) (see 2 papers)
36% identity, 92% coverage: 6:235/251 of query aligns to 16:247/265 of P07821

query
sites
P07821

H

N

L

I

S

S

L

F

S

R

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G

G

K

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A

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G

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K

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T

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I

K50 (= K40) mutation to Q: Lack of activity.
D172 (= D161) mutation to E: Lack of activity.
E173 (= E162) mutation to A: Lack of activity.

4fi3C Structure of vitamin b12 transporter btucd-f in a nucleotide-bound state (see paper)
35% identity, 88% coverage: 23:243/251 of query aligns to 21:239/248 of 4fi3C

query
sites
4fi3C

E

E

L

V

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A

G

G

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E

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H

L

L

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N
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N

G
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G

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A

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G

K
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K

S
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S

S
x
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E

binding phosphoaminophosphonic acid-adenylate ester: N34 (= N36), G35 (= G37), G37 (= G39), K38 (= K40), S39 (= S41), T40 (≠ S42), R121 (= R129), Q125 (≠ R133), S127 (= S135), G129 (= G137), E130 (= E138), Q158 (≠ E162)
binding magnesium ion: S39 (= S41), Q79 (= Q82)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 14

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
32% identity, 85% coverage: 13:226/251 of query aligns to 29:238/378 of P69874

query
sites
P69874

G

G

K

K

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E

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N

D

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F

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E

F45 (≠ V29) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ A38) mutation to T: Loss of ATPase activity and transport.
L60 (= L44) mutation to F: Lower ATPase activity and transport efficiency.
L76 (= L60) mutation to P: Lower ATPase activity and transport efficiency.
V135 (≠ F121) mutation to M: Loss of ATPase activity and transport.
D172 (= D161) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

26 C→A: Lower ATPase activity and transport efficiency.
27 F→L: Lower ATPase activity and transport efficiency.
276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

1l7vC Bacterial abc transporter involved in b12 uptake (see paper)
35% identity, 84% coverage: 23:233/251 of query aligns to 21:229/231 of 1l7vC

query
sites
1l7vC

E

E

L

V

K

R

P

A

G

G

E

E

V

I

T

L

V

H

L

L

L

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G

G

P

P

N
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N

G
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G

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A

G
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G

K
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S
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S

S
x
T

L

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-

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Y

binding cyclo-tetrametavanadate: N34 (= N36), G35 (= G37), A36 (= A38), G37 (= G39), K38 (= K40), S39 (= S41), T40 (≠ S42)

3d31A Modbc from methanosarcina acetivorans (see paper)
32% identity, 86% coverage: 8:224/251 of query aligns to 6:216/348 of 3d31A

query
sites
3d31A

S

S

L

L

S

S

R

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G

W

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K

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N

L

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D

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E

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M

F

Sites not aligning to the query:

binding tungstate(vi)ion: 286, 320, 323, 340, 341, 342

5x40A Structure of a cbio dimer bound with amppcp (see paper)
35% identity, 88% coverage: 17:238/251 of query aligns to 21:240/280 of 5x40A

query
sites
5x40A

L

L

Q

D

G

D

M

L

N

S

L

L

E

A

L

V

K

P

P

K

G

G

E

E

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S

T

L

V

A

L

I

L

L

G

G

P

P

N
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N

G
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G

A

A

G
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G

K
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K

S
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S

S
x
T

L

L

G

L

L

H

L

L

S

N

G

G

Q

T

H

L

S

R

P

P

D

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S

S

G

G

S

R

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V

L

L

D

G

E

G

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A

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A

G

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H

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K

G

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Y

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x
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S

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F

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R

E

A

R

R

V

V

K

E

Y

E

Y

A

L

L

T

A

Q

A

F

L

E

S

L

I

D

S

Q

D

L

L

A

R

Q

D

R

R

Q

P

Y

T

T
x
H

R
x
M

L
|
L

S
|
S

G

G

G
|
G

E
x
Q

Q

K

Q

R

R

R

V

V

Q

A

V

I

A

A

R

G

V

A

M

V

A

A

Q

M

V

-

G

-

D

-

Q

R

C

P

R

E

V

V

L

L

D

D

E
x
Q

P

P

V

T

S

A

E

G

M

L

D

D

L

L

K

A

Y

G

Q

T

Q

E

L

Q

L

L

M

L

Q

T

R

L

I

L

R

R

Q

G

V

L

A

R

A

A

T

A

G

G

V

M

A

T

V

L

C

V

C

F

V

S

L

T

H
|
H

D

D

L

V

N

E

L

L

A

A

Q

A

L

L

A

A

D

D

Q

R

V

V

V

A

L

L

L

F

Q

R

N

T

G

G

Q

R

M

V

L

L

A

A

S

E

G

G

A

A

V

A

D

E

E

A

V

V

M

L

T

S

E

D

S

R

C

A

L

-

S

-

D

T

L

L

Y

A

Q

K

V

V

M

A

V

L

R

R

binding phosphomethylphosphonic acid adenylate ester: N40 (= N36), G41 (= G37), G43 (= G39), K44 (= K40), S45 (= S41), T46 (≠ S42), Q88 (≠ Y80), H139 (≠ T132), M140 (≠ R133), L141 (= L134), S142 (= S135), G144 (= G137), Q145 (≠ E138), Q166 (≠ E162), H198 (= H194)
binding magnesium ion: S45 (= S41), Q88 (≠ Y80)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenylate ester: 14, 18, 20

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
32% identity, 84% coverage: 16:225/251 of query aligns to 17:225/241 of 4u00A

query
sites
4u00A

L
x
V

L

L

Q

K

G

G

M

I

N

H

L

L

E

E

L

V

K

A

P

P

G

G

E

E

V

K

T

L

V

V

L

I

G

G

P

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

S
x
T

L

L

L

I

G

R

L

T

L

I

S

N

G

R

Q

L

H

E

S

D

P

F

D

Q

S

E

G

G

S

E

V

V

L

V

D

D

E

G

Q

L

P

S

V

V

A

K

G

D

L

-

D

D

P

R

Q

A

L

L

R

R

A

E

C

I

Y

R

-

R

-

E

L

V

A

G

M

M

Y

V

T

F

Q

Q

Q

F

P

N

L

L

S

F

F

P

S

H

F

M

R

T

V

V

E

L

E

E

V

N

V

V

A

T

L

L

G

A

C

-

Y

-

P

P

L

M

Q

R

L

V

D

R

A

R

V

W

A

P

M

R

T

E

E

K

R

A

V

E

K

K

Y

K

Y

A

L

L

T

E

Q

L

F

L

E

E

-

R

-

V

-

G

-

I

L

L

D

D

Q

Q

L

-

A

A

Q

R

R

K

Q

Y

Y

P

T

A

R

Q

L

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

Q

A

V

I

A

A

R

R

V

A

M

L

A

A

Q

M

V

-

G

-

D

-

Q

E

C

P

R

K

V

I

L

M

L

L

L

F

D

D

E

E

P

P

V

T

S

S

E

A

M

L

D

D

L

P

K

E

Y

M

Q

V

Q

G

L

E

L

V

M

L

Q

D

R

V

I

M

R

R

Q

D

V

L

A

A

A

Q

T

G

G

G

V

M

A

T

V

M

C

V

V

V

L

T

H

H

D

E

L

M

N

G

L

F

A

A

A

R

Q

E

L

V

A

A

D

D

Q

R

V

V

L

F

L

M

Q

D

N

G

G

G

Q

Q

M

I

L

V

A

E

S

E

G

G

A

R

V

P

D

E

E

E

V

I

M

F

T

T

binding adenosine-5'-diphosphate: V17 (≠ L16), S37 (≠ N36), G38 (= G37), S39 (≠ A38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (≠ S42)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

8t4eB Transporter associated with antigen processing (tap) bound to the 9- mer peptide ilkepvhgv
33% identity, 82% coverage: 10:214/251 of query aligns to 349:550/551 of 8t4eB

query
sites
8t4eB

S

N

R

R

Q

P

G

D

K

R

L

P

L

V

L

L

Q

K

G

G

M

L

N

T

L

F

E

T

L

L

K

R

P

P

G

G

E

E

V

V

T

T

V

A

L

L

L

V

G

G

P

P

N

N

G

G

A

S

G

G

K

K

S

S

S

T

L

V

L

A

G

A

L

L

S

Q

G

N

Q

L

H

Y

S

Q

P

P

D

T

S

G

G

G

S

Q

V

V

L

L

D

D

E

E

Q

K

P

P

V

I

A

S

G

-

L

-

D

-

P

-

Q

Q

L

Y

R

E

A

H

C

C

Y

Y

L

L

-

H

-

S

-

Q

A

V

M

V

Y

S

T

V

Q

G

Q

Q

Q

E

P

P

L

V

S

L

F

F

S

S

F

G

R

S

V

V

E

R

E

N

V

N

V

I

A

A

L

Y

G

G

C

L

Y

Q

P

S

L

C

Q

E

L

D

D

D

A

K

V

V

A

-

M

M

T

A

E

A

R

A

V

Q

K

A

Y

A

Y

H

L

A

T

D

Q

D

F

F

E

-

L

I

D

Q

Q

E

L

M

A

E

Q

H

R

G

Q

I

Y

Y

T

T

-

D

-

V

-

G

-

E

-

K

-

G

-

S

R

Q

L

L

S

A

G

A

G

G

E

Q

Q

K

Q

Q

R

R

V

L

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

D

C

P

R

R

V

V

L

L

L

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D

D

L

V

K

Q

Y

C

Q

E

Q

Q

L

A

L

L

M

Q

Q

D

R

W

I

N

R

S

Q

R

V

G

A

D

A

R

T

T

G

-

V

-

A

-

V

V

C

L

C

V

V

I

L

A

H

H

D

R

L

L

N

Q

L

-

A

T

A

V

Q

Q

L

R

A

A

D

H

Q

Q

V

I

V

L

L

V

L

L

Q

Q

N

E

G

G

Q

K

M

L

Sites not aligning to the query:

binding : 136, 243, 247, 298, 299

Q03519 Antigen peptide transporter 2; APT2; ATP-binding cassette sub-family B member 3; Peptide supply factor 2; Peptide transporter PSF2; PSF-2; Peptide transporter TAP2; Peptide transporter involved in antigen processing 2; Really interesting new gene 11 protein; RING11; EC 7.4.2.14 from Homo sapiens (Human) (see 8 papers)
33% identity, 82% coverage: 10:214/251 of query aligns to 479:680/686 of Q03519

query
sites
Q03519

S

N

R

R

Q

P

G

D

K

R

L

P

L

V

L

L

Q

K

G

G

M

L

N

T

L

F

E

T

L

L

K

R

P

P

G

G

E

E

V

V

T

T

V

A

L

L

L

V

G

G

P

P

N

N

G

G

A

S

G

G

K

K

S

S

S

T

L

V

L

A

G

A

L

L

S

Q

G

N

Q

L

H

Y

S

Q

P

P

D

T

S

G

G

G

S

Q

V

V

L

L

D

D

E

E

Q

K

P

P

V

I

A

S

G

-

L

-

D

-

P

-

Q

Q

L

Y

R

E

A

H

C

C

Y

Y

L

L

-

H

-

S

-

Q

A

V

M

V

Y

S

T

V

Q

G

Q

Q

Q

E

P

P

L

V

S

L

F

F

S

S

F

G

R

S

V

V

E

R

E

N

V

N

V

I

A
|
A

L

Y

G

G

C

L

Y

Q

P

S

L

C

Q

E

L

D

D

D

A

K

V

V

A

-

M
|
M

T

A

E

A

R

A

V

Q

K

A

Y

A

Y

H

L

A

T

D

Q

D

F

F

E

-

L

I

D

Q

Q

E

L

M

A

E

Q

H

R

G

Q

I

Y

Y

T

T

-

D

-

V

-

G

-

E

-

K

-

G

-

S

R

Q

L

L

S

A

G

A

G

G

E

Q

Q

K

Q

Q

R

R

V

L

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

D

C

P

R

R

V

V

L

L

L

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D
|
D

L

V

K

Q

Y

C

Q

E

Q

Q

L

A

L

L

M

Q

Q

D

R

W

I

N

R

S

Q
x
R

V

G

A

D

A

R

T

T

G

-

V

-

A

-

V

V

C

L

C

V

V

I

L

A

H

H

D

R

L

L

N

Q

L

-

A
x
T

A

V

Q

Q

L

R

A

A

D

H

Q

Q

V

I

V

L

L

V

L

L

Q

Q

N

E

G

G

Q

K

M

L

A565 (= A97) to T: in allele TAP2*01:02, allele TAP2*01D, allele TAP2*02E and allele TAP2*02F; dbSNP:rs2228396
M577 (= M110) to V: in allele TAP2*BKY2; dbSNP:rs2228391
D638 (= D168) mutation to A: Inactive in peptide transport when associated with 'A-734' of TAP1.
R651 (≠ Q181) to C: in allele TAP2*01:03 and allele TAP2*01G; dbSNP:rs4148876
T665 (≠ A199) to A: in allele TAP2*02:01, allele TAP2*02B, allele TAP2*02C, allele TAP2*02D, allele TAP2*02E, allele TAP2*02F, allele TAP2*04A and allele TAP2*Bky2; dbSNP:rs241447

Sites not aligning to the query:

16 Inter-subunit salt bridge with TAPBP; D→K: Complete loss of interaction with TAPBP, resulting in impaired PLC assembly and antigen presentation.
301:389 Part of the peptide-binding site
374 A → T: in allele TAP2*01F, allele TAP2*01G, allele TAP2*01H, allele TAP2*02B and allele TAP2*02D; dbSNP:rs111303994
379 V → I: in allele TAP2*01D, allele TAP2*01E, allele TAP2*01G, allele TAP2*02C and allele TAP2*02F; dbSNP:rs1800454
414:433 Part of the peptide-binding site
467 V → I: in allele TAP2*01F and allele TAP2*02D; dbSNP:rs150253319

1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
31% identity, 81% coverage: 20:223/251 of query aligns to 25:216/353 of 1vciA

query
sites
1vciA

M

L

N

N

L

L

E

T

L

I

K

K

P

D

G

G

E

E

V

F

T

L

V

V

L

L

G

G

P

P

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
x
T

L

T

L

L

G

R

L

M

L

I

S

A

G

G

Q

L

H

E

S

E

P

P

D

T

S

E

G

G

S

R

V

I

L

Y

L

F

D

G

E

D

Q

R

P

D

V

V

A

T

G

Y

L

L

D

P

P

P

Q

K

L

D

R

R

A

N

C

-

Y

-

L

-

A

-

M

-

Y

-

T

-

Q

-

P

-

L

I

S

S

F

M

S

V

F

F

R

Q

V

H

E

M

E

T

V

V

V

Y

A

E

L

N

G

I

C

A

Y

F

P

P

L

L

-

K

Q

K

L

F

D

P

A

K

V

D

A

E

M

I

T

D

E

K

R

R

V

V

K

R

Y

W

Y

A

L

A

T

E

Q

L

F

L

E

Q

L

I

D

E

Q

E

L

L

A

L

Q

N

R

R

Q

Y

Y

P

T

A

R

Q

L

L

S

S

G

G

E

Q

Q

R

Q

Q

R

R

V

V

Q

A

V

V

A

A

R

R

V

A

M

I

A

V

Q

V

V

E

G

P

D

D

Q

-

C

-

R

-

V

V

L

L

L

M

D

D

E

E

P

P

V

L

S

S

E

N

M

L

D

D

L

A

K

K

Y

L

Q

R

Q

V

L

A

L

M

M

R

Q

A

R

E

I

I

R

K

Q

K

V

L

A

Q

T

K

-

L

G

K

V

V

A

T

V

T

C

I

C

Y

V

V

L

T

H

H

D

D

L

Q

N

V

L

E

A

A

A

M

Q

T

L

M

A

G

D

D

Q

R

V

I

V

A

L

V

L

M

Q

N

N

R

G

G

Q

Q

M

L

L

L

A

Q

S

I

G

G

A

S

V

P

D

T

E

E

V

V

binding adenosine-5'-triphosphate: S41 (≠ N36), G42 (= G37), G44 (= G39), K45 (= K40), T46 (≠ S41), T47 (≠ S42)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 16, 19

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
29% identity, 80% coverage: 16:216/251 of query aligns to 23:223/650 of 5ws4A

query
sites
5ws4A

L

I

L

L

Q

K

G

G

M

I

N

D

L

L

E

T

L

I

K

Y

P

E

G

G

E

E

V

L

T

V

V

A

L

I

L

V

G

G

P

Q

N
x
S

G
|
G

A

S

G

G

K

K

S
|
S

S

T

L

L

L

M

G

N

L

I

L

L

S

G

G

C

Q

L

H

D

S

R

P

P

D

T

S

S

G

G

S

S

V

Y

L

K

L

V

D

N

E

G

Q

Q

P

E

V

T

A

G

G

K

L

L

D

E

P

P

-

D

-

Q

-

L

-

A

Q

Q

L

L

R

R

A

R

C

E

Y

Y

L

F

A

G

M

F

Y

I

T

F

Q

Q

Q

R

Q

Y

P

H

L

L

S

L

F

G

S

D

F

L

R

S

V

A

E

E

E

G

V

N

V

V

A

E

L

V

G

P

C

A

Y

V

P

Y

L

A

Q

G

L

V

D

T

A

P

V

A

A

D

M

R

T

K

E

Q

R

R

V

A

K

T

Y

A

Y

L

L

L

T

T

Q

E

F

L

E

G

L

L

D

G

Q

T

L

K

A

T

Q

Q

R

N

Q

R

Y

P

T

S

R

Q

L

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

Q

S

V

I

A

A

R

R

V

A

M

L

A

M

Q

N

V

G

G

G

D

D

Q

-

C

-

R

-

V

V

L

I

L

L

L

A

D

D

E

E

P

P

V

T

S

G

E

A

M

L

D

D

L

S

K

H

Y

S

Q

G

Q

V

L

E

L

V

M

M

Q

R

R

I

I

L

R

R

Q

E

V

L

A

N

A

A

T

A

G

G

V

H

A

T

V

I

C

I

C

L

V

V

L

T

H

H

D

D

L

M

N

Q

L

V

A

A

A

K

Q

N

L

-

A

A

D

T

Q

R

V

I

L

E

L

I

Q

S

N

D

G

G

Q

E

M

I

L

I

A

S

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (≠ N36), G44 (= G37), S48 (= S41)

7zk4A The abcb1 l335c mutant (mabcb1) in the outward facing state (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 955:1166/1176 of 7zk4A

query
sites
7zk4A

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

V

K

K

P

K

G

G

E

Q

V

T

T

L

V

A

L

L

L

V

G

G

P

S

N
x
S

G
|
G

A

G

G
|
G

K
|
K

S
|
S

S
x
T

L

V

G

Q

L

L

S

E

G

R

Q

F

H

Y

S

D

P

P

D

M

S

A

G

G

S

S

V

V

L

F

L

L

D

D

E

G

Q

K

P

E

V

I

A

K

G

Q

L

L

D

N

P

V

Q

Q

L

W

R

L

A

R

C

A

Y

Q

L

L

A

G

M

I

Y

V

T

S

Q

-

Q
|
Q

Q

E

P

P

L

I

S

L

F

F

S

D

F

R

R

S

V

I

E

A

E

E

V

N

V

I

A

A

L

Y

G

G

C

D

Y

N

P

S

L

R

Q

V

L

V

D

S

A

Y

V

E

A

E

M

I

T

V

E

R

R

A

V

A

K

K

Y

E

Y

A

L

N

T

I

Q

H

F

Q

E

F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

Q

Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

T

-
x
Q

L
|
L

S
|
S

G
|
G

E
x
Q

Q

K

Q

Q

R

R

V

I

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

P

R

H

V

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D

D

L

T

K

E

Y

S

Q

E

Q

K

L

V

M

Q

Q

E

R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

V

R

A

T

V

V

C

I

C

V

V

I

L

A

H
|
H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

Q

K

M

V

L

K

A

E

S

H

G

G

A

T

V

H

D

Q

E

Q

V

L

M

L

T

A

E

Q

binding adenosine-5'-triphosphate: S975 (≠ N36), G976 (= G37), G978 (= G39), K979 (= K40), S980 (= S41), T981 (≠ S42), Q1021 (= Q83), Q1078 (vs. gap), L1079 (= L134), S1080 (= S135), G1081 (= G136), G1082 (= G137), Q1083 (≠ E138), H1135 (= H194)
binding magnesium ion: S980 (= S41), Q1021 (= Q83)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 364, 367, 372, 392, 393, 395, 396, 397, 398, 438, 493, 495, 496, 497, 498, 947, 949, 950
binding magnesium ion: 397, 438
binding cholesterol hemisuccinate: 6, 7, 14, 15, 16, 16, 17, 71, 75, 79, 82, 98, 101, 156, 318, 831, 839, 854, 861, 861

7zkaA Abcb1 v978c mutant (mabcb1) in the outward facing state bound to aac (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 954:1165/1177 of 7zkaA

query
sites
7zkaA

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

V

K

K

P

K

G

G

E

Q

V

T

T

L

V

A

L

L

L

V

G

G

P

S

N
x
S

G
|
G

A

G

G
|
G

K
|
K

S
|
S

S
x
T

L

V

G

Q

L

L

S

E

G

R

Q

F

H

Y

S

D

P

P

D

M

S

A

G

G

S

S

V

V

L

F

L

L

D

D

E

G

Q

K

P

E

V

I

A

K

G

Q

L

L

D

N

P

V

Q

Q

L

W

R

L

A

R

C

A

Y

Q

L

L

A

G

M

I

Y

V

T

S

Q

-

Q
|
Q

Q

E

P

P

L

I

S

L

F

F

S

D

F

R

R

S

V

I

E

A

E

E

V

N

V

I

A

A

L

Y

G

G

C

D

Y

N

P

S

L

R

Q

V

L

V

D

S

A

Y

V

E

A

E

M

I

T

V

E

R

R

A

V

A

K

K

Y

E

Y

A

L

N

T

I

Q

H

F

Q

E
x
F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

Q

Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

T

-
x
Q

L
|
L

S
|
S

G

G

G
|
G

E
x
Q

Q

K

Q

Q

R

R

V

I

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

P

R

H

V

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D

D

L

T

K

E

Y

S

Q

E

Q

K

L

V

M

Q

Q

E

R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

V

R

A

T

V

V

C

I

C

V

V

I

L

A

H

H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

Q

K

M

V

L

K

A

E

S

H

G

G

A

T

V

H

D

Q

E

Q

V

L

M

L

T

A

E

Q

binding adenosine-5'-triphosphate: S974 (≠ N36), G975 (= G37), G977 (= G39), K978 (= K40), S979 (= S41), T980 (≠ S42), Q1020 (= Q83), F1059 (≠ E122), Q1077 (vs. gap), L1078 (= L134), S1079 (= S135), G1081 (= G137), Q1082 (≠ E138)
binding magnesium ion: S979 (= S41), Q1020 (= Q83)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 364, 366, 372, 392, 393, 395, 396, 397, 398, 438, 493, 495, 497, 498, 946, 948, 949
binding (4~{S},11~{S},18~{S})-4,11-dimethyl-18-(sulfanylmethyl)-6,13,20-trithia-3,10,17,22,23,24-hexazatetracyclo[17.2.1.1^{5,8}.1^{12,15}]tetracosa-1(21),5(24),7,12(23),14,19(22)-hexaene-2,9,16-trione: 31, 35, 38, 81, 299, 303, 884
binding Lauryl Maltose Neopentyl Glycol: 89, 93, 842
binding magnesium ion: 397, 438
binding cholesterol hemisuccinate: 3, 6, 17, 153, 156, 160, 163, 189, 189, 190, 193, 196, 197, 197, 200, 308, 318, 318, 322

7zk6A Abcb1 l335c mutant (mabcb1) in the outward facing state bound to 2 molecules of aac (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 954:1165/1177 of 7zk6A

query
sites
7zk6A

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

V

K

K

P

K

G

G

E

Q

V

T

T

L

V

A

L

L

L

V

G

G

P

S

N
x
S

G
|
G

A

G

G
|
G

K
|
K

S
|
S

S
x
T

L

V

G

Q

L

L

S

E

G

R

Q

F

H

Y

S

D

P

P

D

M

S

A

G

G

S

S

V

V

L

F

L

L

D

D

E

G

Q

K

P

E

V

I

A

K

G

Q

L

L

D

N

P

V

Q

Q

L

W

R

L

A

R

C

A

Y

Q

L

L

A

G

M

I

Y

V

T

S

Q

-

Q
|
Q

Q

E

P

P

L

I

S

L

F

F

S

D

F

R

R

S

V

I

E

A

E

E

V

N

V

I

A

A

L

Y

G

G

C

D

Y

N

P

S

L

R

Q

V

L

V

D

S

A

Y

V

E

A

E

M

I

T

V

E

R

R

A

V

A

K

K

Y

E

Y

A

L

N

T

I

Q

H

F

Q

E

F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

Q

Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

T

-
x
Q

L

L

S
|
S

G
|
G

E
x
Q

Q

K

Q

Q

R

R

V

I

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

P

R

H

V

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D

D

L

T

K

E

Y

S

Q

E

Q

K

L

V

M

Q

Q

E

R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

V

R

A

T

V

V

C

I

C

V

V

I

L

A

H
|
H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

Q

K

M

V

L

K

A

E

S

H

G

G

A

T

V

H

D

Q

E

Q

V

L

M

L

T

A

E

Q

binding adenosine-5'-triphosphate: S974 (≠ N36), G975 (= G37), G977 (= G39), K978 (= K40), S979 (= S41), T980 (≠ S42), Q1020 (= Q83), Q1077 (vs. gap), S1079 (= S135), G1080 (= G136), G1081 (= G137), Q1082 (≠ E138), H1134 (= H194)
binding magnesium ion: S979 (= S41), Q1020 (= Q83)

Sites not aligning to the query:

7zk5A Abcb1 l335c mutant (mabcb1) in the outward facing state bound to aac (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 954:1165/1177 of 7zk5A

query
sites
7zk5A

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

V

K

K

P

K

G

G

E

Q

V

T

T

L

V

A

L

L

L

V

G

G

P

S

N
x
S

G
|
G

A

G

G
|
G

K
|
K

S
|
S

S
x
T

L

V

G

Q

L

L

S

E

G

R

Q

F

H

Y

S

D

P

P

D

M

S

A

G

G

S

S

V

V

L

F

L

L

D

D

E

G

Q

K

P

E

V

I

A

K

G

Q

L

L

D

N

P

V

Q

Q

L

W

R

L

A

R

C

A

Y

Q

L

L

A

G

M

I

Y

V

T

S

Q

-

Q
|
Q

Q

E

P

P

L

I

S

L

F

F

S

D

F

R

R

S

V

I

E

A

E

E

V

N

V

I

A

A

L

Y

G

G

C

D

Y

N

P

S

L

R

Q

V

L

V

D

S

A

Y

V

E

A

E

M

I

T

V

E

R

R

A

V

A

K

K

Y

E

Y

A

L

N

T

I

Q

H

F

Q

E

F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

Q

Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

T

-
x
Q

L
|
L

S
|
S

G

G

G
|
G

E
x
Q

Q

K

Q

Q

R

R

V

I

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

P

R

H

V

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D

D

L

T

K

E

Y

S

Q

E

Q

K

L

V

M

Q

Q

E

R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

V

R

A

T

V

V

C

I

C

V

V

I

L

A

H
|
H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

Q

K

M

V

L

K

A

E

S

H

G

G

A

T

V

H

D

Q

E

Q

V

L

M

L

T

A

E

Q

binding adenosine-5'-triphosphate: S974 (≠ N36), G975 (= G37), G977 (= G39), K978 (= K40), S979 (= S41), T980 (≠ S42), Q1020 (= Q83), Q1077 (vs. gap), L1078 (= L134), S1079 (= S135), G1081 (= G137), Q1082 (≠ E138), H1134 (= H194)
binding magnesium ion: S979 (= S41), Q1020 (= Q83)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 364, 366, 367, 372, 392, 393, 395, 396, 397, 398, 438, 493, 495, 496, 497, 498, 550, 946, 948, 949
binding (4~{S},11~{S},18~{S})-4,11-dimethyl-18-(sulfanylmethyl)-6,13,20-trithia-3,10,17,22,23,24-hexazatetracyclo[17.2.1.1^{5,8}.1^{12,15}]tetracosa-1(21),5(24),7,12(23),14,19(22)-hexaene-2,9,16-trione: 31, 45, 299, 302, 303, 306, 630, 881, 884, 885, 888
binding magnesium ion: 397, 438
binding cholesterol hemisuccinate: 3, 6, 7, 14, 17, 79, 98, 156, 164, 185, 186, 189, 193, 194, 197, 318, 830, 834, 838, 850, 853, 860

6fn1A Zosuquidar and uic2 fab complex of human-mouse chimeric abcb1 (abcb1hm) (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 958:1169/1182 of 6fn1A

query
sites
6fn1A

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

V

K

K

P

K

G

G

E

Q

V

T

T

L

V

A

L

L

L

V

G

G

P

S

N

S

G

G

A

A

G

G

K

K

S

S

S

T

L

V

G

Q

L

L

S

E

G

R

Q

F

H

Y

S

D

P

P

D

M

S

A

G

G

S

S

V

V

L

F

L

L

D

D

E

G

Q

K

P

E

V

I

A

K

G

Q

L

L

D

N

P

V

Q

Q

L

W

R

L

A

R

C

A

Y

Q

L

L

A

G

M

I

Y

V

T

S

Q

-

Q

Q

Q

E

P

P

L

I

S

L

F

F

S

D

F

T

R

S

V

I

E

A

E

E

V

N

V

I

A

A

L

Y

G

G

C

D

Y

N

P

S

L

R

Q

V

L

V

D

S

A

Y

V

E

A

E

M

I

T

V

E

R

R

A

V

A

K

K

Y

E

Y

A

L

N

T

I

Q

H

F

Q

E

F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

Q

Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

T

-

Q

L

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

I

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

P

R

H

V

I

L

L

D

D

E

E

P

A

V

T

S

C

E

A

M

L

D

D

L

T

K

E

Y

S

Q

E

Q

K

L

V

M

Q

Q

E

R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

V

R

A

T

V

T

C

I

C

V

V

I

L

A

H

H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

Q

K

M

V

L

K

A

E

S

H

G

G

A

T

V

H

D

Q

E

Q

V

L

M

L

T

A

E

Q

Sites not aligning to the query:

binding Zosuquidar: 34, 201, 202, 272, 308, 312, 781, 785, 855, 856, 892, 893, 896, 897

8wm7D Cryo-em structure of cyanobacterial nitrate/nitrite transporter nrtbcd in complex with signalling protein pii (see paper)
31% identity, 82% coverage: 16:220/251 of query aligns to 23:222/257 of 8wm7D

query
sites
8wm7D

L
x
V

L

L

Q

D

G

G

M

I

N

D

L

L

E

K

L

V

K

R

P

E

G

G

E

E

V

F

T

V

V

C

L

L

L

I

G

G

P

H

N
x
S

G
|
G

A
x
C

G
|
G

K
|
K

S
|
S

S
x
T

L

L

G

N

L

M

L

I

S

S

G

G

Q

F

H

N

S

T

P

P

D

S

S

E

G

G

S

V

V

V

L

L

D

Q

E

D

Q

K

P

P

V

I

A

T

G

E

L

P

D

G

P

P

Q

D

L

-

R

-

A

-

C

-

Y

-

L

R

A

M

M

M

Y

V

T

F

Q

Q

Q

N

Q

Y

P

C

L

L

S

L

F

P

S

W

F

L

R

N

V

V

E

F

E

E

V

N

V

V

A

Y

L

L

G

A

C

V

Y

D

P

A

L

V

Q

F

L

P

D

N

A

K

V

P

A

Q

M

A

T

E

E

K

R

R

-

A

-

I

V

V

K

R

Y

E

Y

H

L

L

T

A

Q

M

F

V

E

G

L

L

D

T

Q

E

L

A

A

A

Q

E

R

K

Q

K

Y

P

T

S

R

Q

L

I

S

S

G

G

E

M

Q

K

Q

Q

R

R

V

V

Q

A

V

I

A

A

R

R

V

A

M

L

A

S

Q

I

V

-

G

-

D

-

Q

R

C

P

R

Q

V

V

L

L

L

I

L

L

D

D

E

E

P

P

V

F

S

G

E

A

M

L

D

D

L

A

K

I

Y

T

Q

K

Q

E

L

E

L

L

M

Q

Q

E

R

E

I

L

R

L

Q

Q

V

I

A

W

A

S

T

D

G

H

-

Q

V

V

A

T

V

V

C

L

C

M

V

I

L

T

H

H

D

D

L

I

N

D

L

E

A

A

A

L

Q

F

L

L

A

A

D

D

Q

R

V

V

L

M

Q

T

N

N

G

G

Q

P

M

A

L

A

A

Q

S

I

G

G

A

E

V

I

binding adenosine-5'-diphosphate: V23 (≠ L16), S43 (≠ N36), G44 (= G37), C45 (≠ A38), G46 (= G39), K47 (= K40), S48 (= S41), T49 (≠ S42)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 14, 21

7otgA Structure of abcb1/p-glycoprotein in the presence of the cftr potentiator ivacaftor (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 956:1167/1179 of 7otgA

query
sites
7otgA

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

V

K

K

P

K

G

G

E

Q

V

T

T

L

V

A

L

L

L

V

G

G

P

S

N

S

G

G

A

C

G

G

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K

S

S

S

T

L

V

G

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L

L

S

E

G

R

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F

H

Y

S

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P

P

D

M

S

A

G

G

S

S

V

V

L

F

L

L

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D

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G

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K

P

E

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I

A

K

G

Q

L

L

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N

P

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L

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R

L

A

R

C

A

Y

Q

L

L

A

G

M

I

Y

V

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S

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-

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Q

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E

P

P

L

I

S

L

F

F

S

D

F

C

R

S

V

I

E

A

E

E

V

N

V

I

A

A

L

Y

G

G

C

D

Y

N

P

S

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L

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S

A

Y

V

E

A

E

M

I

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V

E

R

R

A

V

A

K

K

Y

E

Y

A

L

N

T

I

Q

H

F

Q

E

F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

Q

Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

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-

Q

L

L

S

S

G

G

E

Q

Q

K

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R

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I

Q

A

V

I

A

A

R

R

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A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

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R

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I

L

L

D

D

E

E

P

A

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T

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S

E

A

M

L

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D

L

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Y

S

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E

Q

K

L

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M

Q

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R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

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R

A

T

V

C

C

I

C

V

V

I

L

A

H

H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

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M

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G

G

A

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V

H

D

Q

E

Q

V

L

M

L

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E

Q

Sites not aligning to the query:

binding N-(2,4-di-tert-butyl-5-hydroxyphenyl)-4-oxo-1,4-dihydroquinoline-3-carboxamide: 307, 311, 779, 780

6q81A Structure of p-glycoprotein(abcb1) in the post-hydrolytic state (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 959:1170/1182 of 6q81A

query
sites
6q81A

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

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K

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K

G

G

E

Q

V

T

T

L

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A

L

L

L

V

G

G

P

S

N
x
S

G
|
G

A

C

G
|
G

K
|
K

S
|
S

S

T

L

V

G

Q

L

L

S

E

G

R

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F

H

Y

S

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P

D

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V

L

F

L

L

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D

E

G

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K

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E

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I

A

K

G

Q

L

L

D

N

P

V

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Q

L

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R

L

A

R

C

A

Y

Q

L

L

A

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M

I

Y

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-

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E

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P

L

I

S

L

F

F

S

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F

C

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I

E

A

E

E

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N

V

I

A

A

L

Y

G

G

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Y

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L

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S

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E

A

E

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I

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R

A

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A

K

K

Y

E

Y

A

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N

T

I

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H

F

Q

E

F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

Q

Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

T

-

Q

L

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

I

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

P

R

H

V

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D

D

L

T

K

E

Y

S

Q

E

Q

K

L

V

M

Q

Q

E

R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

V

R

A

T

V

C

C

I

C

V

V

I

L

A

H

H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

Q

K

M

V

L

K

A

E

S

H

G

G

A

T

V

H

D

Q

E

Q

V

L

M

L

T

A

E

Q

binding adenosine-5'-diphosphate: S979 (≠ N36), G980 (= G37), G982 (= G39), K983 (= K40), S984 (= S41)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 365, 370, 394, 396, 397, 398, 712, 951

3g61A Structure of p-glycoprotein reveals a molecular basis for poly- specific drug binding (see paper)
29% identity, 84% coverage: 16:226/251 of query aligns to 959:1170/1182 of 3g61A

query
sites
3g61A

L

V

L

L

Q

Q

G

G

M

L

N

S

L

L

E

E

L

V

K

K

P

K

G

G

E

Q

V

T

T

L

V

A

L

L

L

V

G

G

P

S

N

S

G

G

A

C

G

G

K

K

S

S

S

T

L

V

G

Q

L

L

S

E

G

R

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F

H

Y

S

D

P

P

D

M

S

A

G

G

S

S

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V

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F

L

L

D

D

E

G

Q

K

P

E

V

I

A

K

G

Q

L

L

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N

P

V

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Q

L

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R

L

A

R

C

A

Y

Q

L

L

A

G

M

I

Y

V

T

S

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-

Q

Q

Q

E

P

P

L

I

S

L

F

F

S

D

F

C

R

S

V

I

E

A

E

E

V

N

V

I

A

A

L

Y

G

G

C

D

Y

N

P

S

L

R

Q

V

L

V

D

S

A

Y

V

E

A

E

M

I

T

V

E

R

R

A

V

A

K

K

Y

E

Y

A

L

N

T

I

Q

H

F

Q

E

F

L

I

D

D

Q

S

L

L

A

P

Q

D

R

K

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Y

Y

N

T

T

R

R

-

V

-

G

-

D

-

K

-

G

-

T

-

Q

L

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

I

Q

A

V

I

A

A

R

R

V

A

M

L

A

V

Q

R

V

-

G

-

D

-

Q

Q

C

P

R

H

V

I

L

L

D

D

E

E

P

A

V

T

S

S

E

A

M

L

D

D

L

T

K

E

Y

S

Q

E

Q

K

L

V

M

Q

Q

E

R

A

I

L

R

D

Q

K

V

-

A

A

A

R

T

E

G

G

V

R

A

T

V

C

C

I

C

V

V

I

L

A

H

H

D

R

L

L

N

S

L

-

A

T

A

I

Q

Q

L

N

A

A

D

D

Q

L

V

I

V

V

L

V

L

I

Q

Q

N

N

G

G

Q

K

M

V

L

K

A

E

S

H

G

G

A

T

V

H

D

Q

E

Q

V

L

M

L

T

A

E

Q

Sites not aligning to the query:

binding (4S,11S,18S)-4,11,18-tri(propan-2-yl)-6,13,20-triselena-3,10,17,22,23,24-hexaazatetracyclo[17.2.1.1~5,8~.1~12,15~]tetracosa-1(21),5(24),7,12(23),14,19(22)-hexaene-2,9,16-trione: 39, 268, 271, 300, 307, 635, 639, 860, 885, 889, 889

Query Sequence

>WP_019623149.1 NCBI__GCF_000381785.1:WP_019623149.1
MLTASHLSLSRQGKLLLQGMNLELKPGEVTVLLGPNGAGKSSLLGLLSGQHSPDSGSVLL
DEQPVAGLDPQLRACYLAMYTQQQPLSFSFRVEEVVALGCYPLQLDAVAMTERVKYYLTQ
FELDQLAQRQYTRLSGGEQQRVQVARVMAQVGDQCRVLLLDEPVSEMDLKYQQLLMQRIR
QVAATGVAVCCVLHDLNLAAQLADQVVLLQNGQMLASGAVDEVMTESCLSDLYQVMVRKV
DTETGPLFSSR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory