SitesBLAST
Comparing WP_019623244.1 NCBI__GCF_000381785.1:WP_019623244.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 18 hits to proteins with known functional sites (download)
P38502 Acetate kinase; Acetokinase; EC 2.7.2.1 from Methanosarcina thermophila (see 5 papers)
36% identity, 99% coverage: 4:394/394 of query aligns to 3:397/408 of P38502
- N7 (= N8) mutation to A: Almost abolishes catalytic activity. Requires increased magnesium levels for activity. Strongly decreases affinity for acetate.; mutation to D: Almost abolishes catalytic activity. Strongly decreases affinity for acetate.
- S10 (= S11) mutation S->A,T: Strongly decreases catalytic activity. Strongly decreases affinity for acetate.
- S12 (= S13) mutation to A: Decreases catalytic activity. Strongly decreases affinity for acetate. Requires increased magnesium levels for enzyme activity.; mutation to T: Decreases catalytic activity. Strongly decreases affinity for acetate.
- K14 (= K15) mutation to A: Strongly decreases enzyme activity.; mutation to R: Reduces enzyme activity.
- R91 (= R95) mutation R->A,L: Decreases catalytic activity. Decreases affinity for acetate.
- V93 (= V97) mutation to A: Decreases affinity for acetate.
- L122 (= L126) mutation to A: Decreases affinity for acetate.
- D148 (= D152) active site, Proton donor/acceptor; mutation D->A,E,N: Abolishes catalytic activity. Decreases affinity for acetate, but not for ATP.
- F179 (= F182) mutation to A: Decreases affinity for acetate.
- N211 (≠ A213) mutation to A: Slightly reduced enzyme activity.
- P232 (≠ A234) mutation to A: Decreases affinity for acetate.
- R241 (= R243) mutation R->K,L: Decreases catalytic activity. Strongly reduced affinity for ATP.
- E384 (= E381) mutation to A: Almost abolishes catalytic activity. Strongly decreases affinity for acetate. Requires strongly increased magnesium levels for enzyme activity.
1tuuB Acetate kinase crystallized with atpgs (see paper)
36% identity, 99% coverage: 4:394/394 of query aligns to 3:397/398 of 1tuuB
- active site: N7 (= N8), R91 (= R95), H180 (= H183), R241 (= R243), E384 (= E381)
- binding adenosine monophosphate: D283 (= D285), R285 (≠ L287), G331 (= G330), I332 (≠ V331), N335 (= N334), S336 (≠ D335)
- binding trihydrogen thiodiphosphate: H180 (= H183), G212 (= G214), R241 (= R243)
1tuuA Acetate kinase crystallized with atpgs (see paper)
37% identity, 99% coverage: 4:394/394 of query aligns to 3:397/399 of 1tuuA
- active site: N7 (= N8), R91 (= R95), H180 (= H183), R241 (= R243), E384 (= E381)
- binding adenosine-5'-diphosphate: K14 (= K15), G210 (= G212), D283 (= D285), F284 (≠ M286), R285 (≠ L287), G331 (= G330), I332 (≠ V331), N335 (= N334)
- binding sulfate ion: R91 (= R95), H180 (= H183), G212 (= G214)
4fwsA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with ctp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwsA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding cytidine-5'-triphosphate: G202 (= G212), N203 (≠ A213), G204 (= G214), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G323 (= G330), I324 (≠ V331), N327 (= N334)
- binding 1,2-ethanediol: V21 (≠ R21), C24 (= C28), H115 (= H127), N203 (≠ A213), T232 (≠ S242), R233 (= R243), K262 (= K272)
4fwrA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with cmp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwrA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding cytidine-5'-monophosphate: G202 (= G212), N203 (≠ A213), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G323 (= G330), I324 (≠ V331), N327 (= N334)
4fwqA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gtp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwqA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding guanosine-5'-triphosphate: H172 (= H183), N203 (≠ A213), G204 (= G214), D275 (= D285), L276 (≠ M286), R277 (≠ L287), E280 (= E290), G323 (= G330), I324 (≠ V331), N327 (= N334)
4fwpA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gdp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwpA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding 1,2-ethanediol: S11 (= S11), H115 (= H127), K262 (= K272)
- binding guanosine-5'-diphosphate: N203 (≠ A213), D275 (= D285), L276 (≠ M286), R277 (≠ L287), E280 (= E290), G323 (= G330), I324 (≠ V331), N327 (= N334), S328 (≠ D335)
4fwoA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with gmp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwoA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding guanosine-5'-monophosphate: G202 (= G212), N203 (≠ A213), D275 (= D285), L276 (≠ M286), R277 (≠ L287), E280 (= E290), G323 (= G330), I324 (≠ V331), N327 (= N334)
- binding 1,2-ethanediol: E100 (≠ Q112), N104 (≠ Q116)
4fwnA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with adenosine tetraphosphate (ap4) (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwnA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding adenosine-5'-tetraphosphate: H172 (= H183), H200 (= H210), N203 (≠ A213), G204 (= G214), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G323 (= G330), I324 (≠ V331), N327 (= N334)
4fwmA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with atp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwmA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding adenosine-5'-triphosphate: H172 (= H183), H200 (= H210), N203 (≠ A213), G204 (= G214), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G323 (= G330), I324 (≠ V331), N327 (= N334)
- binding 1,2-ethanediol: H172 (= H183), R233 (= R243)
4fwkA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with amp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 4fwkA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding adenosine monophosphate: G202 (= G212), N203 (≠ A213), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G323 (= G330), I324 (≠ V331), N327 (= N334)
- binding 1,2-ethanediol: D103 (≠ Q115), N104 (≠ Q116), R107 (≠ E119)
2e1zA Crystal structure of salmonella typhimurium propionate kinase (tdcd) in complex with diadenosine tetraphosphate (ap4a) obtained after co- crystallization with atp (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 2e1zA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding bis(adenosine)-5'-tetraphosphate: N8 (= N8), R83 (= R95), H115 (= H127), G202 (= G212), N203 (≠ A213), G204 (= G214), P224 (≠ A234), R233 (= R243), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G323 (= G330), I324 (≠ V331), N327 (= N334)
1x3nA Crystal structure of amppnp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 1x3nA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding phosphoaminophosphonic acid-adenylate ester: G202 (= G212), N203 (≠ A213), G204 (= G214), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G323 (= G330), I324 (≠ V331), N327 (= N334)
1x3mA Crystal structure of adp bound propionate kinase (tdcd) from salmonella typhimurium (see paper)
37% identity, 98% coverage: 3:387/394 of query aligns to 3:384/394 of 1x3mA
- active site: N8 (= N8), R83 (= R95), H172 (= H183), R233 (= R243), E378 (= E381)
- binding adenosine-5'-diphosphate: G202 (= G212), N203 (≠ A213), D275 (= D285), L276 (≠ M286), R277 (≠ L287), G322 (= G329), G323 (= G330), I324 (≠ V331), N327 (= N334)
7fj9A Kpacka (pduw) with amppnp complex structure
36% identity, 97% coverage: 4:386/394 of query aligns to 4:385/395 of 7fj9A
7fj8A Kpacka (pduw) with amp complex structure
36% identity, 97% coverage: 4:386/394 of query aligns to 4:385/395 of 7fj8A
4ijnA Crystal structure of an acetate kinase from mycobacterium smegmatis bound to amp and sulfate (see paper)
37% identity, 98% coverage: 1:386/394 of query aligns to 1:370/376 of 4ijnA
- active site: N8 (= N8), R72 (= R95), H161 (= H183), R222 (= R243), E365 (= E381)
- binding adenosine monophosphate: G191 (= G212), N192 (≠ A213), D263 (vs. gap), F264 (vs. gap), R265 (≠ A284), G311 (= G330), V312 (= V331), N315 (= N334), V316 (≠ D335)
4iz9A Crystal structure of an acetate kinase from mycobacterium avium bound to an unknown acid-apcpp conjugate and manganese (see paper)
35% identity, 99% coverage: 4:393/394 of query aligns to 6:379/381 of 4iz9A
- active site: N10 (= N8), R74 (= R95), H163 (= H183), R224 (= R243), E367 (= E381)
- binding diphosphomethylphosphonic acid adenosyl ester: K17 (= K15), G193 (= G212), N194 (≠ A213), D265 (vs. gap), F266 (vs. gap), R267 (≠ A284), G313 (= G330), I314 (≠ V331), N317 (= N334), D318 (= D335)
Query Sequence
>WP_019623244.1 NCBI__GCF_000381785.1:WP_019623244.1
MSLILTVNGGSSSLKCALFSRHTGNAECLYQFKLANILNAPVMKIVSSLGEILEQHQLDM
AEIATDNRHQACLDQVIQWVHKQLPDSRISAFGHRVVHGGEAFSDPVQITDQIMQQLEEF
IPLAPLHQPYNLKLIQACQHLEPALPQIACFDTMFHIGQPNVERHYAIPRKYTEAGIHKY
GFHGLSYEFIQRSLDNIGRGAENAKTIVCHLGAGASMCAVNNGRSIASTMGFTAVDGLPM
GSRTGNIDPGVLLYLQRHYQMDADQLESFLYKESGWLGVSGISADMLELEQSEAPEAEEA
IAMFAYRAALEIGRLSAALQGLEQLVFTGGVGENDNKLRKRVCDQLEWLGIKLNESANKE
GEAIISKASSPVVVRVIPTNEEAMIALHTEEVLD
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory