SitesBLAST
Comparing WP_019744147.1 NCBI__GCF_000691145.1:WP_019744147.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
67% identity, 99% coverage: 5:485/486 of query aligns to 3:481/487 of P42412
- C36 (≠ A38) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R109) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T152) binding NAD(+)
- F152 (= F154) binding NAD(+)
- C160 (= C162) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K178) binding NAD(+)
- E179 (= E181) binding NAD(+)
- R180 (= R182) binding NAD(+)
- S229 (= S231) binding NAD(+)
- T251 (≠ A253) binding NAD(+)
- R283 (= R285) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ A289) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ R353) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E386) binding NAD(+)
- C413 (= C417) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
67% identity, 99% coverage: 5:485/486 of query aligns to 1:479/484 of 1t90A
- active site: N151 (= N155), K174 (= K178), L248 (= L252), C282 (= C286), E380 (= E386), A460 (= A466)
- binding nicotinamide-adenine-dinucleotide: I147 (= I151), A148 (≠ T152), P149 (= P153), F150 (= F154), N151 (= N155), W159 (= W163), K174 (= K178), E177 (= E181), R178 (= R182), H207 (= H211), V225 (= V229), G226 (= G230), S227 (= S231), V230 (= V234), L248 (= L252), T249 (≠ A253), C282 (= C286), E380 (= E386), F382 (= F388)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
48% identity, 98% coverage: 8:485/486 of query aligns to 3:481/489 of 4zz7A
- active site: N149 (= N155), K172 (= K178), L246 (= L252), C280 (= C286), E382 (= E386), A462 (= A466)
- binding nicotinamide-adenine-dinucleotide: T146 (= T152), P147 (= P153), F148 (= F154), N149 (= N155), K172 (= K178), E175 (= E181), K205 (≠ H211), V208 (= V214), F222 (= F228), V223 (= V229), G224 (= G230), S225 (= S231), I228 (≠ V234), L246 (= L252), G247 (≠ A253), C280 (= C286), E382 (= E386), F384 (= F388)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
46% identity, 98% coverage: 9:485/486 of query aligns to 3:455/468 of 5tjrD
- active site: N144 (= N155), K167 (= K178), L241 (= L252), C270 (= C286), E356 (= E386), A436 (= A466)
- binding adenosine-5'-diphosphate: I140 (= I151), T141 (= T152), F143 (= F154), K167 (= K178), E170 (= E181), K200 (≠ H211), F217 (= F228), S220 (= S231), I223 (≠ V234)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
45% identity, 98% coverage: 9:485/486 of query aligns to 7:485/491 of 4iymC
- active site: N153 (= N155), K176 (= K178), F250 (≠ L252), C284 (= C286), E386 (= E386), Q466 (≠ A466)
- binding nicotinamide-adenine-dinucleotide: I149 (= I151), T150 (= T152), P151 (= P153), F152 (= F154), N153 (= N155), F154 (= F156), K176 (= K178), K209 (≠ H211), V212 (= V214), F226 (= F228), V227 (= V229), G228 (= G230), S229 (= S231), I232 (≠ V234), G251 (≠ A253), C284 (= C286), E386 (= E386), F388 (= F388)
1bpwA Betaine aldehyde dehydrogenase from cod liver (see paper)
31% identity, 97% coverage: 11:481/486 of query aligns to 22:492/503 of 1bpwA
- active site: N166 (= N155), K189 (= K178), E263 (≠ L252), C297 (= C286), E400 (= E386), E477 (≠ A466)
- binding nicotinamide-adenine-dinucleotide: I162 (= I151), L163 (≠ T152), W165 (≠ F154), N166 (= N155), K189 (= K178), G221 (≠ A210), G225 (≠ V214), T240 (≠ V229), G241 (= G230), S242 (= S231), T245 (≠ V234), E263 (≠ L252), L264 (≠ A253), C297 (= C286), E400 (= E386), F402 (= F388), F466 (= F453)
P56533 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABADH; Aldehyde dehydrogenase family 9 member A1; Betaine aldehyde dehydrogenase; BADH; EC 1.2.1.47; EC 1.2.1.3 from Gadus morhua subsp. callarias (Baltic cod) (Gadus callarias) (see paper)
31% identity, 97% coverage: 11:481/486 of query aligns to 22:492/503 of P56533
O14293 Putative aldehyde dehydrogenase-like protein C9E9.09c; EC 1.2.1.- from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
34% identity, 99% coverage: 3:481/486 of query aligns to 17:493/503 of O14293
- S248 (= S231) modified: Phosphoserine
Sites not aligning to the query:
- 501 modified: Phosphoserine
7radA Crystal structure analysis of aldh1b1
34% identity, 99% coverage: 5:484/486 of query aligns to 9:487/493 of 7radA
- binding nicotinamide-adenine-dinucleotide: I158 (= I151), I159 (≠ T152), P160 (= P153), W161 (≠ F154), N162 (= N155), M167 (≠ V160), K185 (= K178), E188 (= E181), G218 (≠ H211), G222 (≠ N215), A223 (≠ G216), T237 (≠ V229), G238 (= G230), S239 (= S231), V242 (= V234), E261 (≠ L252), L262 (≠ A253), C295 (= C286), E392 (= E386), F394 (= F388)
- binding 3-(2-methoxyphenyl)-1-(4-phenylphenyl)-6,7,8,9-tetrahydro-5~{H}-imidazo[1,2-a][1,3]diazepine: L113 (vs. gap), E117 (≠ R109), F163 (= F156), E285 (≠ G276), F289 (≠ G280), N450 (≠ P445), V452 (≠ P447)
7mjdA Crystal structure analysis of aldh1b1
34% identity, 99% coverage: 5:484/486 of query aligns to 9:487/493 of 7mjdA