SitesBLAST
Comparing WP_019747919.1 NCBI__GCF_002893965.1:WP_019747919.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1mtoA Crystal structure of a phosphofructokinase mutant from bacillus stearothermophilus bound with fructose-6-phosphate (see paper)
42% identity, 97% coverage: 2:335/343 of query aligns to 3:311/319 of 1mtoA
- active site: G11 (= G10), R72 (= R73), C73 (≠ V74), D103 (≠ E104), G104 (= G105), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D128), R162 (= R163), M169 (= M170), R171 (= R172), E222 (= E223), R243 (= R267), H249 (= H273), R252 (= R276)
6pfkA Phosphofructokinase, inhibited t-state (see paper)
42% identity, 97% coverage: 2:335/343 of query aligns to 3:311/319 of 6pfkA
- active site: G11 (= G10), R72 (= R73), C73 (≠ V74), D103 (≠ E104), G104 (= G105), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding 2-phosphoglycolic acid: R21 (= R20), R25 (= R24), G58 (≠ N59), D59 (≠ R60), R154 (≠ H155), R211 (= R212), K213 (≠ D214)
P00512 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see 2 papers)
42% identity, 97% coverage: 2:335/343 of query aligns to 3:311/319 of P00512
- RSVVR 21:25 (≠ RAVVR 20:24) binding ADP
- D59 (≠ R60) binding ADP
- RC 72:73 (≠ RV 73:74) binding ATP
- D103 (≠ E104) binding Mg(2+)
- TID 125:127 (= TID 126:128) binding in other chain
- R154 (≠ H155) binding in other chain
- R162 (= R163) binding substrate
- MGR 169:171 (= MGR 170:172) binding in other chain
- GAE 185:187 (≠ GAH 186:188) binding in other chain
- R211 (= R212) binding in other chain
- KKH 213:215 (≠ DSH 214:216) binding in other chain
- E222 (= E223) binding in other chain
- R243 (= R267) binding substrate
- HVQR 249:252 (≠ HIQR 273:276) binding in other chain
4pfkA Phosphofructokinase. Structure and control (see paper)
42% identity, 97% coverage: 2:335/343 of query aligns to 3:311/319 of 4pfkA
- active site: G11 (= G10), R72 (= R73), C73 (≠ V74), D103 (≠ E104), G104 (= G105), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding adenosine-5'-diphosphate: S9 (≠ G8), Y41 (≠ W41), R72 (= R73), C73 (≠ V74), F76 (≠ D77), K77 (= K78), G104 (= G105), G108 (≠ A109), R154 (≠ H155), G185 (= G186), R211 (= R212), G212 (= G213), K213 (≠ D214), H215 (= H216)
- binding 6-O-phosphono-beta-D-fructofuranose: D127 (= D128), M169 (= M170), E222 (= E223), H249 (= H273), R252 (= R276)
- binding magnesium ion: G185 (= G186), E187 (≠ H188)
3pfkA Phosphofructokinase. Structure and control (see paper)
42% identity, 97% coverage: 2:335/343 of query aligns to 3:311/319 of 3pfkA
- active site: G11 (= G10), R72 (= R73), C73 (≠ V74), D103 (≠ E104), G104 (= G105), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding phosphate ion: R154 (≠ H155), K213 (≠ D214), H249 (= H273), R252 (= R276)
4i4iA Crystal structure of bacillus stearothermophilus phosphofructokinase mutant t156a bound to pep (see paper)
42% identity, 97% coverage: 2:335/343 of query aligns to 3:311/319 of 4i4iA
- active site: G11 (= G10), R72 (= R73), C73 (≠ V74), D103 (≠ E104), G104 (= G105), G124 (≠ K125), T125 (= T126), D127 (= D128), D129 (= D130), R171 (= R172)
- binding phosphoenolpyruvate: R21 (= R20), R25 (= R24), G58 (≠ N59), R154 (≠ H155), R211 (= R212), K213 (≠ D214), H215 (= H216)
1pfkA Crystal structure of the complex of phosphofructokinase from escherichia coli with its reaction products (see paper)
42% identity, 95% coverage: 2:328/343 of query aligns to 4:305/320 of 1pfkA
- active site: G12 (= G10), R73 (= R73), F74 (≠ V74), D104 (≠ E104), G105 (= G105), G125 (≠ K125), T126 (= T126), D128 (= D128), D130 (= D130), R172 (= R172)
- binding adenosine-5'-diphosphate: G11 (= G9), R22 (= R20), R26 (= R24), Y56 (≠ D55), S59 (≠ N59), D60 (≠ R60), R73 (= R73), F74 (≠ V74), F77 (≠ K78), R78 (≠ L79), G103 (= G103), D104 (≠ E104), G105 (= G105), S106 (≠ T106), M108 (≠ T108), G109 (≠ A109), R155 (≠ H155), G213 (= G213), K214 (≠ D214), H216 (= H216)
- binding 1,6-di-O-phosphono-beta-D-fructofuranose: G12 (= G10), R73 (= R73), T126 (= T126), D128 (= D128), M170 (= M170), E223 (= E223), H250 (= H273), R253 (= R276)
- binding magnesium ion: G186 (= G186), E188 (≠ H188)
P0A796 ATP-dependent 6-phosphofructokinase isozyme 1; ATP-PFK 1; Phosphofructokinase 1; 6-phosphofructokinase isozyme I; Phosphohexokinase 1; Sedoheptulose-7-phosphate kinase; EC 2.7.1.11 from Escherichia coli (strain K12) (see 2 papers)
42% identity, 95% coverage: 2:328/343 of query aligns to 4:305/320 of P0A796
- G12 (= G10) binding ATP
- RGVVR 22:26 (≠ RAVVR 20:24) binding ADP
- RYS-VSD 55:60 (≠ SDDRINR 54:60) binding ADP
- RF 73:74 (≠ RV 73:74) binding ATP
- GDGS 103:106 (≠ GEGT 103:106) binding ATP
- D104 (≠ E104) binding Mg(2+)
- TID 126:128 (= TID 126:128) binding in other chain
- D128 (= D128) active site, Proton acceptor; mutation to S: 18000-fold reduction of catalytic rate.
- R155 (≠ H155) binding in other chain
- R163 (= R163) binding substrate
- R172 (= R172) mutation to S: 3.4-fold reduction in turnover numbers.
- GCE 186:188 (≠ GAH 186:188) binding in other chain
- KKH 214:216 (≠ DSH 214:216) binding in other chain
- E223 (= E223) binding in other chain
- R244 (= R267) binding substrate
- HIQR 250:253 (= HIQR 273:276) binding in other chain
5xzaA Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adp (see paper)
38% identity, 92% coverage: 2:315/343 of query aligns to 3:293/322 of 5xzaA
Q2FXM8 ATP-dependent 6-phosphofructokinase; ATP-PFK; Phosphofructokinase; Phosphohexokinase; EC 2.7.1.11 from Staphylococcus aureus (strain NCTC 8325 / PS 47) (see paper)
38% identity, 92% coverage: 2:315/343 of query aligns to 3:293/322 of Q2FXM8
- RC 72:73 (≠ RV 73:74) binding ATP
- GDGS 102:105 (≠ GEGT 103:106) binding ATP
- TID 127:129 (= TID 126:128) binding in other chain
- G150 (≠ D149) mutation to D: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of dimer conversion; in association with A-151.
- L151 (≠ A150) mutation to A: Exhibits higher affinity for fructose 6-phosphate and higher catalytic activity with a loss of tetramer-dimer conversion; in association with D-150.
- R164 (= R163) mutation to A: Complete loss of fructose 6-phosphate binding.
- MGR 171:173 (= MGR 170:172) binding in other chain
- E224 (= E223) binding in other chain
- R245 (= R267) mutation to A: Complete loss of fructose 6-phosphate binding.
- HVQR 251:254 (≠ HIQR 273:276) binding in other chain
5xz6A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 92% coverage: 2:315/343 of query aligns to 3:293/318 of 5xz6A
5xz9A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 92% coverage: 2:315/343 of query aligns to 3:293/321 of 5xz9A
- binding adenosine-5'-triphosphate: S9 (≠ G8), Y41 (≠ W41), C73 (≠ V74), F76 (≠ D77), K77 (= K78), G102 (= G103), D103 (≠ E104), G104 (= G105), S105 (≠ T106), R107 (≠ T108), G108 (≠ A109)
5xz7A Crystal structure of phosphofructokinase from staphylococcus aureus in complex with adenylylimidodiphosphate, the atp analogue (see paper)
38% identity, 92% coverage: 2:315/343 of query aligns to 3:293/322 of 5xz7A
Q8TGA0 ATP-dependent 6-phosphofructokinase subunit beta; ATP-dependent 6-phosphofructokinase 2; ATP-PFK 2; Phosphofructokinase 2; Phosphohexokinase 2; EC 2.7.1.11 from Komagataella pastoris (Yeast) (Pichia pastoris) (see 2 papers)
35% identity, 96% coverage: 3:332/343 of query aligns to 184:528/941 of Q8TGA0