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Comparing WP_019867191.1 NCBI__GCF_000384075.1:WP_019867191.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7caxD Crystal structure of bacterial reductase
64% identity, 99% coverage: 3:240/241 of query aligns to 2:237/238 of 7caxD
- active site: G12 (= G13), S139 (= S140), Y152 (= Y153)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (= S11), R11 (= R12), I13 (= I14), H31 (= H32), R33 (= R34), S34 (= S35), R35 (≠ Q36), D59 (= D60), V60 (≠ I61), N86 (= N87), A87 (= A88), G88 (= G89), S139 (= S140), Y152 (= Y153), K156 (= K157), P182 (= P183), G183 (= G184), I185 (= I186)
4iiuA Crystal structure of a putative 3-oxoacyl-[acyl-carrier protein]reductase from escherichia coli strain cft073 complexed with NADP+ at 2.1 a resolution
59% identity, 100% coverage: 1:240/241 of query aligns to 1:242/243 of 4iiuA
- active site: G13 (= G13), S140 (= S140), Y153 (= Y153), K157 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G9), S11 (= S11), K12 (≠ R12), G13 (= G13), I14 (= I14), H32 (= H32), H34 (≠ R34), D36 (≠ Q36), D60 (= D60), V61 (≠ I61), N87 (= N87), A88 (= A88), G89 (= G89), I90 (= I90), S140 (= S140), Y153 (= Y153), K157 (= K157), P183 (= P183), G184 (= G184), I186 (= I186), T188 (= T188), M190 (= M190), I191 (vs. gap)
3osuA Crystal structure of the 3-oxoacyl-acyl carrier protein reductase, fabg, from staphylococcus aureus
45% identity, 100% coverage: 1:240/241 of query aligns to 3:244/246 of 3osuA
3sj7A Structure of beta-ketoacetyl-coa reductase (fabg) from staphylococcus aureus complex with NADPH (see paper)
45% identity, 99% coverage: 3:240/241 of query aligns to 2:237/239 of 3sj7A
- active site: G12 (= G13), S138 (= S140), Q148 (= Q150), Y151 (= Y153), K155 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (= S11), R11 (= R12), I13 (= I14), N31 (≠ H32), Y32 (≠ C33), A33 (≠ R34), G34 (≠ S35), S35 (≠ Q36), A58 (≠ F59), N59 (≠ D60), V60 (≠ I61), N86 (= N87), A87 (= A88), T109 (= T110), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184)
3op4A Crystal structure of putative 3-ketoacyl-(acyl-carrier-protein) reductase from vibrio cholerae o1 biovar eltor str. N16961 in complex with NADP+ (see paper)
43% identity, 99% coverage: 3:240/241 of query aligns to 9:244/247 of 3op4A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G15 (= G9), S17 (= S11), R18 (= R12), I20 (= I14), T40 (≠ R34), N62 (≠ D60), V63 (≠ I61), N89 (= N87), A90 (= A88), I92 (= I90), V139 (≠ L138), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), I187 (= I186), T189 (= T188), M191 (= M190)
4i08A Crystal structure of beta-ketoacyl-acyl carrier protein reductase (fabg) from vibrio cholerae in complex with NADPH (see paper)
43% identity, 99% coverage: 3:240/241 of query aligns to 9:240/243 of 4i08A
- active site: G19 (= G13), N113 (= N111), S141 (= S140), Q151 (= Q150), Y154 (= Y153), K158 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G15 (= G9), S17 (= S11), R18 (= R12), I20 (= I14), T40 (≠ R34), N62 (≠ D60), V63 (≠ I61), N89 (= N87), A90 (= A88), G140 (≠ S139), S141 (= S140), Y154 (= Y153), K158 (= K157), P184 (= P183), G185 (= G184), T189 (= T188)
1q7bA The structure of betaketoacyl-[acp] reductase from e. Coli in complex with NADP+ (see paper)
44% identity, 99% coverage: 3:240/241 of query aligns to 5:240/243 of 1q7bA
- active site: G15 (= G13), E101 (= E103), S137 (= S140), Q147 (= Q150), Y150 (= Y153), K154 (= K157)
- binding calcium ion: E232 (≠ Q232), T233 (≠ V233)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G9), S13 (= S11), R14 (= R12), T36 (≠ R34), N58 (≠ D60), V59 (≠ I61), N85 (= N87), A86 (= A88), G87 (= G89), I88 (= I90), S137 (= S140), Y150 (= Y153), K154 (= K157), P180 (= P183), G181 (= G184), I183 (= I186)
P0AEK2 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Escherichia coli (strain K12) (see 2 papers)
44% identity, 99% coverage: 3:240/241 of query aligns to 6:241/244 of P0AEK2
- GASR 12:15 (≠ GSSR 9:12) binding NADP(+)
- T37 (≠ R34) binding NADP(+)
- NV 59:60 (≠ DI 60:61) binding NADP(+)
- N86 (= N87) binding NADP(+)
- Y151 (= Y153) mutation to F: Defect in the affinity for NADPH.
- YAAAK 151:155 (≠ YSAAK 153:157) binding NADP(+)
- A154 (= A156) mutation to T: Decreases in the thermolability of the reductase; when associated with K-233.
- K155 (= K157) mutation to A: Defect in the affinity for NADPH.
- I184 (= I186) binding NADP(+)
- E233 (≠ Q232) mutation to K: Decreases in the thermolability of the reductase; when associated with T-154.
1q7cA The structure of betaketoacyl-[acp] reductase y151f mutant in complex with NADPH fragment (see paper)
43% identity, 99% coverage: 3:240/241 of query aligns to 5:240/243 of 1q7cA
- active site: G15 (= G13), S137 (= S140), Q147 (= Q150), F150 (≠ Y153), K154 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G9), S13 (= S11), R14 (= R12), A35 (≠ C33), T36 (≠ R34), L57 (≠ F59), N58 (≠ D60), V59 (≠ I61), G87 (= G89), I88 (= I90)
6t77A Crystal structure of klebsiella pneumoniae fabg(NADPH-dependent) NADP- complex at 1.75 a resolution (see paper)
43% identity, 99% coverage: 3:240/241 of query aligns to 6:241/244 of 6t77A
- active site: G16 (= G13), S138 (= S140), Y151 (= Y153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (= S11), R15 (= R12), T37 (≠ R34), L58 (≠ F59), N59 (≠ D60), V60 (≠ I61), A87 (= A88), G88 (= G89), I89 (= I90)
7emgB Carbonyl reductase variant 4 (r123c/l209p/f183y/v61k) from serratia marcescens complexed with NADP+ (see paper)
43% identity, 99% coverage: 3:240/241 of query aligns to 5:240/243 of 7emgB
P0A2C9 3-oxoacyl-[acyl-carrier-protein] reductase FabG; 3-ketoacyl-acyl carrier protein reductase; Beta-Ketoacyl-acyl carrier protein reductase; Beta-ketoacyl-ACP reductase; EC 1.1.1.100 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
42% identity, 99% coverage: 3:240/241 of query aligns to 6:241/244 of P0A2C9
- M125 (= M126) mutation to I: Loss of the temperature-sensitive phenotype; when associated with T-223.
- A223 (≠ M222) mutation to T: Loss of the temperature-sensitive phenotype; when associated with I-125.
- S224 (= S223) mutation to F: Distorts the local conformation and prevent stacking around Phe-221. The S224F mutation would additionally disrupt the hydrogen bond formed between Ser-224 and Glu-226.
4k6fB X-ray crystal structure of a putative acetoacetyl-coa reductase from burkholderia cenocepacia bound to the co-factor NADP
41% identity, 99% coverage: 2:239/241 of query aligns to 1:241/245 of 4k6fB
- active site: G12 (= G13), N102 (≠ E103), S138 (= S140), Y151 (= Y153), K155 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), Y32 (≠ C33), S33 (≠ R34), N36 (≠ K37), V58 (≠ F59), D59 (= D60), V60 (≠ I61), A87 (= A88), G88 (= G89), I89 (= I90)
P73574 3-oxoacyl-[acyl-carrier-protein] reductase; 3-ketoacyl-acyl carrier protein reductase; EC 1.1.1.100 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
43% identity, 98% coverage: 6:241/241 of query aligns to 10:245/247 of P73574
- A14 (≠ S10) mutation to G: 4.2-fold increase in activity on acetoacetyl-CoA.
- P151 (≠ R148) mutation to F: 2.7-fold increase in activity on acetoacetyl-CoA.; mutation to V: 5.7-fold increase in activity on acetoacetyl-CoA.
- K160 (= K157) mutation to A: Almost no activity on acetoacetyl-CoA.
- F188 (≠ L185) mutation to Y: 3.3-fold increase in activity on acetoacetyl-CoA.
- N198 (≠ P195) mutation to R: 3.5-fold increase in activity on acetoacetyl-CoA.
1edoA The x-ray structure of beta-keto acyl carrier protein reductase from brassica napus complexed with NADP+ (see paper)
44% identity, 98% coverage: 5:240/241 of query aligns to 4:242/244 of 1edoA
- active site: G12 (= G13), S138 (= S140), Y151 (= Y153), K155 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), S10 (= S11), R11 (= R12), I13 (= I14), N31 (≠ L31), Y32 (≠ H32), A33 (≠ C33), R34 (= R34), S35 (= S35), D59 (= D60), V60 (≠ I61), N86 (= N87), A87 (= A88), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), I184 (= I186), S186 (≠ T188), M188 (= M190)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
41% identity, 99% coverage: 3:241/241 of query aligns to 6:246/247 of 4jroC
- active site: G16 (= G13), S142 (= S140), Q152 (= Q150), Y155 (= Y153), K159 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (= S11), R15 (= R12), G16 (= G13), I17 (= I14), N35 (≠ H32), Y36 (≠ C33), N37 (≠ R34), G38 (≠ S35), S39 (≠ Q36), N63 (≠ D60), V64 (≠ I61), N90 (= N87), A91 (= A88), I93 (= I90), I113 (≠ T110), S142 (= S140), Y155 (= Y153), K159 (= K157), P185 (= P183), I188 (= I186), T190 (= T188)
6t62A Crystal structure of acinetobacter baumannii fabg in complex with NADPH at 1.8 a resolution (see paper)
40% identity, 99% coverage: 2:240/241 of query aligns to 5:241/244 of 6t62A
- active site: G16 (= G13), S138 (= S140), Y151 (= Y153)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (= S11), R15 (= R12), A36 (≠ R34), T37 (≠ S35), L58 (≠ F59), D59 (= D60), V60 (≠ I61), N86 (= N87), A87 (= A88), G88 (= G89), I89 (= I90), I136 (≠ L138), S137 (= S139), S138 (= S140), Y151 (= Y153), K155 (= K157), P181 (= P183), G182 (= G184), I184 (= I186), M188 (= M190)
6wprA Crystal structure of a putative 3-oxoacyl-acp reductase (fabg) with NADP(h) from acinetobacter baumannii (see paper)
40% identity, 99% coverage: 2:240/241 of query aligns to 5:241/244 of 6wprA
- active site: G16 (= G13), S138 (= S140), Y151 (= Y153)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G12 (= G9), S14 (= S11), R15 (= R12), T37 (≠ S35), L58 (≠ F59), D59 (= D60), V60 (≠ I61), N86 (= N87), A87 (= A88), G88 (= G89), I89 (= I90), I136 (≠ L138), Y151 (= Y153), K155 (= K157), P181 (= P183)
5vt6A Crystal structure of acetoacetyl-coa reductase from burkholderia pseudomallei 1710b complexed with NADP
40% identity, 99% coverage: 2:240/241 of query aligns to 1:242/245 of 5vt6A
- active site: G12 (= G13), D102 (≠ E103), S138 (= S140), Y151 (= Y153), K155 (= K157)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), G11 (≠ R12), G12 (= G13), L13 (≠ I14), H32 (≠ C33), S33 (≠ R34), N36 (≠ K37), V58 (≠ F59), D59 (= D60), V60 (≠ I61), N86 (= N87), A87 (= A88), G88 (= G89), I89 (= I90), I136 (≠ L138), Y151 (= Y153), K155 (= K157), P181 (= P183), Y183 (≠ L185), L184 (≠ I186), T186 (= T188)
4ag3A Crystal structure of 3-ketoacyl-(acyl-carrier-protein) reductase (fabg) from pseudomonas aeruginosa in complex with NADPH at 1.8a resolution (see paper)
42% identity, 99% coverage: 3:240/241 of query aligns to 13:251/254 of 4ag3A
- active site: G23 (= G13), S148 (= S140), Y161 (= Y153), K165 (= K157)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G19 (= G9), S21 (= S11), R22 (= R12), G23 (= G13), I24 (= I14), T44 (≠ S35), L68 (≠ F59), D69 (= D60), V70 (≠ I61), N96 (= N87), A97 (= A88), I146 (≠ L138), S148 (= S140), Y161 (= Y153), K165 (= K157), P191 (= P183), G192 (= G184), F193 (≠ L185), I194 (= I186), T196 (= T188), M198 (= M190), T199 (≠ A191)
Query Sequence
>WP_019867191.1 NCBI__GCF_000384075.1:WP_019867191.1
MKKTILVTGSSRGIGKAIALYLAKQGYDLVLHCRSQKARADEVIAEIEQLGSSARLLQFD
IGDRGQCAGQLNQDIERHGAYYGVVCNAGITADNAFPSLTGEEWDSVIHTNLDGFYNVLQ
PVIMPMVRRRQPGRIVTLSSVSGIMGNRGQVNYSAAKAGIIGATKALALELAKRDITVNC
VAPGLIATDMADGLPLDEIKKMIPARRVGDPKEVAAAVAFLMSEDAGYITRQVLSVNGGL
C
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory