SitesBLAST
Comparing WP_019894044.1 NCBI__GCF_000384235.1:WP_019894044.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
53% identity, 99% coverage: 4:503/506 of query aligns to 15:512/514 of P04968
- K62 (= K51) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N78) binding
- GGGGL 188:192 (= GGGGL 177:181) binding
- S315 (= S304) binding
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
52% identity, 99% coverage: 4:503/506 of query aligns to 11:492/494 of 1tdjA
- active site: K58 (= K51), A83 (= A76), E209 (= E202), S213 (≠ A206), C215 (≠ S208), G237 (= G230), L310 (≠ V303), S311 (= S304)
- binding pyridoxal-5'-phosphate: F57 (= F50), K58 (= K51), N85 (= N78), G184 (= G177), G185 (= G178), G186 (= G179), G187 (= G180), G237 (= G230), E282 (= E275), S311 (= S304), G312 (= G305)
2gn2A Crystal structure of tetrameric biodegradative threonine deaminase (tdcb) from salmonella typhimurium in complex with cmp at 2.5a resolution (hexagonal form) (see paper)
38% identity, 59% coverage: 20:317/506 of query aligns to 25:322/326 of 2gn2A
- active site: K56 (= K51), A81 (= A76), Q207 (≠ E202), V211 (≠ A206), G213 (≠ S208), G235 (= G230), I308 (≠ V303), S309 (= S304)
- binding cytidine-5'-monophosphate: R51 (≠ P46), T52 (≠ V47), G53 (≠ F48), A114 (= A109), D117 (≠ N112), Y118 (≠ L113), N312 (= N307)
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
36% identity, 59% coverage: 18:315/506 of query aligns to 20:313/319 of 2zr8A
- active site: K53 (= K51), S78 (≠ A76), E204 (= E202), G208 (≠ A206), D210 (≠ S208), G232 (= G230), I303 (≠ V303), S304 (= S304)
- binding magnesium ion: E204 (= E202), G208 (≠ A206), D210 (≠ S208)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F50), K53 (= K51), S77 (= S75), S78 (≠ A76), N80 (= N78), H81 (= H79), P147 (= P145), G179 (= G177), G180 (= G178), G181 (= G179), G182 (= G180), G232 (= G230), E277 (= E275), T279 (≠ A277), S304 (= S304)
- binding serine: S78 (≠ A76), R129 (≠ A127), D231 (= D229), G232 (= G230), A233 (≠ V231), Q234 (≠ A232), T235 (≠ V233)
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
36% identity, 59% coverage: 18:315/506 of query aligns to 20:313/319 of 2zpuA
- active site: K53 (= K51), S78 (≠ A76), E204 (= E202), G208 (≠ A206), D210 (≠ S208), G232 (= G230), I303 (≠ V303), S304 (= S304)
- binding magnesium ion: E204 (= E202), G208 (≠ A206), D210 (≠ S208)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F50), K53 (= K51), S77 (= S75), S78 (≠ A76), N80 (= N78), H81 (= H79), P147 (= P145), G179 (= G177), G180 (= G178), G181 (= G179), G182 (= G180), G232 (= G230), E277 (= E275), T279 (≠ A277), S304 (= S304)
O59791 Serine racemase; D-serine ammonia-lyase; D-serine dehydratase; L-serine ammonia-lyase; L-serine dehydratase; EC 4.3.1.17; EC 4.3.1.18; EC 5.1.1.18 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 59% coverage: 18:315/506 of query aligns to 24:317/323 of O59791
- S82 (≠ A76) mutation to A: Loss of racemase activity. Reduces D-serine dehydratase activity by 99%. Slightly reduced L-serine dehydratase activity.
1wtcA Crystal structure of s.Pombe serine racemase complex with amppcp (see paper)
36% identity, 59% coverage: 18:315/506 of query aligns to 19:312/318 of 1wtcA
- active site: K52 (= K51), S77 (≠ A76), E203 (= E202), G207 (≠ A206), D209 (≠ S208), G231 (= G230), I302 (≠ V303), S303 (= S304)
- binding phosphomethylphosphonic acid adenylate ester: N20 (≠ I19), K47 (≠ P46), M48 (≠ V47), A109 (≠ N108), A110 (= A109), Y114 (≠ L113)
- binding magnesium ion: E203 (= E202), G207 (≠ A206), D209 (≠ S208)
- binding pyridoxal-5'-phosphate: F51 (= F50), K52 (= K51), N79 (= N78), G178 (= G177), G179 (= G178), G180 (= G179), G181 (= G180), G231 (= G230), E276 (= E275), T278 (≠ A277), S303 (= S304)
1v71A Crystal structure of s.Pombe serine racemase
36% identity, 59% coverage: 18:315/506 of query aligns to 19:312/318 of 1v71A
- active site: K52 (= K51), S77 (≠ A76), E203 (= E202), G207 (≠ A206), D209 (≠ S208), G231 (= G230), I302 (≠ V303), S303 (= S304)
- binding magnesium ion: E203 (= E202), G207 (≠ A206), D209 (≠ S208)
- binding pyridoxal-5'-phosphate: F51 (= F50), K52 (= K51), N79 (= N78), G178 (= G177), G179 (= G178), G180 (= G179), G181 (= G180), G231 (= G230), E276 (= E275), T278 (≠ A277), S303 (= S304), G304 (= G305)
A4F2N8 L-threo-3-hydroxyaspartate ammonia-lyase; L-threo-3-hydroxyaspartate dehydratase; L-THA DH; EC 4.3.1.16 from Pseudomonas sp. (see paper)
36% identity, 58% coverage: 18:311/506 of query aligns to 20:311/319 of A4F2N8
- K53 (= K51) mutation to A: Loss of enzymatic activity.
Q7XSN8 Serine racemase; D-serine dehydratase; D-serine ammonia-lyase; L-serine dehydratase; L-serine ammonia-lyase; EC 5.1.1.18; EC 4.3.1.18; EC 4.3.1.17 from Oryza sativa subsp. japonica (Rice) (see paper)
34% identity, 58% coverage: 20:313/506 of query aligns to 37:332/339 of Q7XSN8
- E219 (= E202) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-225.
- D225 (≠ S208) mutation to A: Reduces catalytic activity and abolishes the regulatory effect of Mg(2+) addition; when associated with A-219.
1ve5A Crystal structure of t.Th. Hb8 threonine deaminase
42% identity, 53% coverage: 42:311/506 of query aligns to 41:307/308 of 1ve5A
- active site: K50 (= K51), S56 (≠ N57), S72 (≠ A76), E200 (= E202), A204 (= A206), D206 (≠ S208), G229 (= G230), L299 (≠ V303), S300 (= S304)
- binding calcium ion: E200 (= E202), A204 (= A206), D206 (≠ S208)
- binding pyridoxal-5'-phosphate: F49 (= F50), K50 (= K51), N74 (= N78), G175 (= G177), G176 (= G178), G177 (= G179), G178 (= G180), E274 (= E275), T276 (≠ A277), S300 (= S304), G301 (= G305)
3l6bA X-ray crystal structure of human serine racemase in complex with malonate a potent inhibitor (see paper)
33% identity, 58% coverage: 21:312/506 of query aligns to 24:314/322 of 3l6bA
- active site: K54 (= K51), S77 (≠ A76), E203 (= E202), A207 (= A206), D209 (≠ S208), G232 (= G230), T278 (≠ A277), L305 (≠ V303), S306 (= S304)
- binding malonate ion: K54 (= K51), S76 (= S75), S77 (≠ A76), N79 (= N78), H80 (= H79), R128 (≠ A128), G232 (= G230)
- binding manganese (ii) ion: E203 (= E202), A207 (= A206), D209 (≠ S208)
- binding pyridoxal-5'-phosphate: F53 (= F50), K54 (= K51), N79 (= N78), G178 (= G177), G179 (= G178), G180 (= G179), G181 (= G180), M182 (≠ L181), V233 (= V231), E276 (= E275), T278 (≠ A277), S306 (= S304), G307 (= G305)
6zspAAA serine racemase bound to atp and malonate. (see paper)
32% identity, 58% coverage: 21:312/506 of query aligns to 23:311/320 of 6zspAAA
- active site: K53 (= K51), S74 (≠ A76), E200 (= E202), A204 (= A206), D206 (≠ S208), G229 (= G230), L302 (≠ V303), S303 (= S304)
- binding adenosine-5'-triphosphate: S28 (≠ A26), S29 (= S27), I30 (≠ L28), K48 (≠ P46), T49 (≠ V47), Q79 (= Q81), Y111 (≠ L113), E266 (= E268), R267 (≠ D269), K269 (≠ R271), N306 (= N307)
- binding magnesium ion: E200 (= E202), A204 (= A206), D206 (≠ S208)
- binding malonate ion: K53 (= K51), S73 (= S75), S74 (≠ A76), N76 (= N78), H77 (= H79), R125 (≠ A128), G229 (= G230), S232 (≠ A234)
7nbhAAA structure of human serine racemase in complex with DSiP fragment Z26781964, XChem fragment screen (see paper)
33% identity, 58% coverage: 21:312/506 of query aligns to 23:318/320 of 7nbhAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (≠ V303), S310 (= S304)
- binding calcium ion: E207 (= E202), A211 (= A206), D213 (≠ S208)
- binding N-[(1H-benzimidazol-2-yl)methyl]furan-2-carboxamide: S81 (≠ A76), G85 (≠ A80), Q86 (= Q81), K111 (= K106), I115 (≠ V110), Y118 (≠ L113), D235 (= D229), P281 (= P276), N313 (= N307), V314 (≠ I308), D315 (≠ N309)
7nbgAAA structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
33% identity, 58% coverage: 21:312/506 of query aligns to 23:318/322 of 7nbgAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (≠ V303), S310 (= S304)
- binding calcium ion: E207 (= E202), A211 (= A206), D213 (≠ S208)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G177), G183 (= G178), G184 (= G179), G185 (= G180), M186 (≠ L181), G236 (= G230), V237 (= V231), T282 (≠ A277), S310 (= S304), G311 (= G305)
- binding ~{N}-[2-(2-methylphenyl)ethyl]ethanamide: S81 (≠ A76), G85 (≠ A80), Q86 (= Q81), I101 (= I96), K111 (= K106), I115 (≠ V110), Y118 (≠ L113)
7nbfAAA structure of human serine racemase in complex with DSiP fragment Z126932614, XChem fragment screen (see paper)
33% identity, 58% coverage: 21:312/506 of query aligns to 23:318/323 of 7nbfAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (≠ V303), S310 (= S304)
- binding calcium ion: E207 (= E202), A211 (= A206), D213 (≠ S208)
- binding magnesium ion: N244 (≠ A239)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G177), G183 (= G178), G184 (= G179), G185 (= G180), M186 (≠ L181), G236 (= G230), V237 (= V231), T282 (≠ A277), S310 (= S304), G311 (= G305)
- binding 2-[(methylsulfonyl)methyl]-1H-benzimidazole: T23 (= T21), P24 (= P22), L26 (≠ E24), T27 (≠ R25), F46 (≠ L44)
Sites not aligning to the query:
7nbdAAA structure of human serine racemase in complex with DSiP fragment Z235449082, XChem fragment screen (see paper)
33% identity, 58% coverage: 21:312/506 of query aligns to 23:318/323 of 7nbdAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (≠ V303), S310 (= S304)
- binding calcium ion: E207 (= E202), A211 (= A206), D213 (≠ S208)
- binding [4-(1H-benzimidazol-1-yl)phenyl]methanol: W272 (≠ F267), L278 (≠ V273), V314 (≠ I308), L316 (≠ F310)
- binding magnesium ion: N244 (≠ A239)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G177), G183 (= G178), G184 (= G179), G185 (= G180), M186 (≠ L181), G236 (= G230), V237 (= V231), E280 (= E275), T282 (≠ A277), S310 (= S304), G311 (= G305)
Sites not aligning to the query:
7nbcCCC structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
33% identity, 58% coverage: 21:312/506 of query aligns to 23:318/323 of 7nbcCCC
- active site: K53 (= K51), S81 (≠ A76), E207 (= E202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (≠ V303), S310 (= S304)
- binding biphenyl-4-ylacetic acid: T78 (≠ A73), H79 (≠ A74), H84 (= H79), V148 (≠ I143), H149 (≠ P144), P150 (= P145)
- binding calcium ion: E207 (= E202), A211 (= A206), D213 (≠ S208)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G177), G183 (= G178), G184 (= G179), G185 (= G180), M186 (≠ L181), G236 (= G230), V237 (= V231), T282 (≠ A277), S310 (= S304), G311 (= G305)
7nbcAAA structure of human serine racemase in complex with DSiP fragment Z2856434779, XChem fragment screen (see paper)
33% identity, 58% coverage: 21:312/506 of query aligns to 23:318/323 of 7nbcAAA
- active site: K53 (= K51), S81 (≠ A76), E207 (= E202), A211 (= A206), D213 (≠ S208), G236 (= G230), L309 (≠ V303), S310 (= S304)
- binding calcium ion: E207 (= E202), A211 (= A206), D213 (≠ S208)
- binding magnesium ion: N244 (≠ A239)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N83 (= N78), G182 (= G177), G183 (= G178), G184 (= G179), G185 (= G180), M186 (≠ L181), G236 (= G230), V237 (= V231), T282 (≠ A277), S310 (= S304), G311 (= G305)
Sites not aligning to the query:
7nbgDDD structure of human serine racemase in complex with DSiP fragment Z52314092, XChem fragment screen (see paper)
33% identity, 57% coverage: 21:309/506 of query aligns to 23:310/310 of 7nbgDDD
- active site: K53 (= K51), S76 (≠ A76), E202 (= E202), A206 (= A206), D208 (≠ S208), G231 (= G230), L304 (≠ V303), S305 (= S304)
- binding calcium ion: E202 (= E202), A206 (= A206), D208 (≠ S208)
- binding magnesium ion: N239 (≠ A239)
- binding ortho-xylene: S76 (≠ A76), Q81 (= Q81), I96 (= I96), Y113 (≠ L113)
- binding pyridoxal-5'-phosphate: F52 (= F50), K53 (= K51), N78 (= N78), G177 (= G177), G178 (= G178), G179 (= G179), G180 (= G180), M181 (≠ L181), G231 (= G230), V232 (= V231), E275 (= E275), T277 (≠ A277), S305 (= S304), G306 (= G305)
Sites not aligning to the query:
Query Sequence
>WP_019894044.1 NCBI__GCF_000384235.1:WP_019894044.1
MPHQILRQVLRAPVYDVAIQTPFERASLISKKANNDVWLKREDLQPVFSFKLRGAYNRMV
QLSDAEKAKGVVAASAGNHAQGVALSASKMGIKALIVMPRTTPMIKVNAVRNLGGEVILH
GDAFDQAAEHARFLCERDGYAFIPPYDDEAVIAGQGTIALEILRQHKPLDVMFVCVGGGG
LIAGVSAVMKQVSPETRIVGVEPEDAASMTAALKAGKRVKLDTVGIFADGVAVAQVGEAP
FRIARQCIDEMITVTTDEICAAVKDIFEDTRAVSEPAGALGLAGMKKYIARTGVSGQSMG
VIVSGANINFDSLRYISERTEIGERREGIFAVVIDERPGSFKRFCELLGAKRAITEFNYR
YADDRKAVVFVGVRTEAGVAEKDQLIAQLKAEDYTVHDMSDNEMAKLHLRHMVGGHAKGV
TNECLYRFQFPERPGALLQFLSQMSEDWNISLFHYRNHGAAYGRVLVGIQVPPDQQSEFE
EYLNCLHYPFESEQNNIGYELFLKPV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory