SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (vs. gap) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D33) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ Y79) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ K104) mutation to H: Little effect on the kinetic properties.
E349 (≠ Q380) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 80% coverage: 23:516/618 of query aligns to 24:441/557 of P78753

query
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P78753

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S391 (≠ Q443) modified: Phosphoserine

Sites not aligning to the query:

489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
28% identity, 63% coverage: 1:392/618 of query aligns to 1:377/561 of P08243

query
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P08243

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C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ G229) to E: in dbSNP:rs1049674
F362 (≠ M377) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
28% identity, 63% coverage: 2:392/618 of query aligns to 1:364/509 of 6gq3A

query
sites
6gq3A

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L

R

Q

T

T

F

F

S

A

I

I

G

G

F

M

E

E

D

D

V

S

P

P

E

D

E

L

K

L

G

A

S

A

E

R

F

-

D

-

Y

-

S

-

D

-

Q

K

V

V

V

A

E

D

R

H

F

I

Q

G

T

S

R

E

H

H

Q

Y

K

E

F

V

L

L

I

F

P

N

N

S

E

E

A

E

V

G

L

I

P

Q

R

A

L

L

Q

D

E

E

A

V

V

I

D

F

A

S

M

L

S

E

-

T

-

Y

E

D

P

I

M
x
T

F

T

A

V

Q
x
R

D

A

A

S

V

V

A

G

F

M

Y

Y

L

L

L

I

S

S

E

K

Q

Y

V

I

S

R

K

K

E

N

V

T

K

D

-

S

-

V

V

V

V

I

M

F

S

S

G

G

Q

E

G

G

A

S

D

D

E

E

V

L

F

T

G

Q

G

G

Y

Y

F

I

W

Y

Y

F

active site: W4 (≠ C5), L49 (= L50), N74 (= N73), G75 (= G74), T324 (≠ M354), R327 (≠ Q357)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R49), V51 (≠ I52), V52 (≠ I53), Y73 (≠ F72), N74 (= N73), G75 (= G74), E76 (≠ C75), V95 (≠ S97), D96 (= D98)

1jgtB Crystal structure of beta-lactam synthetase (see paper)
27% identity, 52% coverage: 69:388/618 of query aligns to 69:353/500 of 1jgtB

query
sites
1jgtB

T

T

L

A

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

Y

-

V

-

A

-

L

-

R

R

E

D

Q

E

L

L

I

L

E

S

L

V

G

L

H

P

A

A

F

G

R

P

S

A

-

P

H

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

R

E

Q

R

W

Y

G

D

M

L

E

H

C

A

V

F

T

R

R

L

F

V

E

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

D

-

H

-

Q

G

H

D

Q

R

L

V

M

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

A

C

P

V

V

A

E

P

G

G

G

E

V

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

A

A

A

A

-

G

-

V

-

N

-

I

-

D

H

L

R

D

D

P

P

V

K

G

G

-

F

-

P

L

L

H

A

H

D

Q

A

F

R

T

R

L

V

H

A

G

G

V

-

P

-

A

-

P

-

H

-

T

-

V

-

L

L

K

T

G

G

V

V

R

Y

K

Q

L

V

A

P

P

A

G

G

Q

A

W

V

M

M

T

D

V

I

N

D

-

L

-

G

P

S

D

G

G

T

Q

A

M

V

Y

T

Q

H

R

R

S

T

W

W

W

T

H

P

L

G

K

L

A

S

E

R

R

R

P

I

G

L

P

P

D

E

H

G

P

E

S

A

S

-

R

-

L

-

Q

-

A

-

L

-

S

-

G

-

V

-

T

-

D

-

H

-

Q

-

A

-

W

-

V

V

E

A

A

A

V

V

H

R

D

A

T

A

L

L

K

E

E

K

A

A

V

V

H

A

K

Q

R

R

L

V

T

T

A

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

L

G

I

V

V

A

A

A

L

C

L

A

D

H

E

R

A

A

G

A

V

G

E

E

D

-

I

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

-

E

-

D

-

V

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

D

R

Y

E

S

A

D

R

Q

A

V

V

E

D

R

H

F

L

Q

R

T

T

R

R

H

H

Q

R

K

E

F

I

L

T

I

I

P

P

N

T

E

T

A

E

V

L

L

L

P

A

R

Q

L

L

Q

P

E

Y

A

A

V

V

D

W

A

A

M

S

S

E

-

S

-

V

E

D

P

P

M
x
D

F

I

A

I

Q

E

D
x
Y

A

L

V

L

A

P

F
x
L

Y

T

L

A

L

L

S

Y

E

R

Q

A

V

L

S

D

K

G

E

P

V

E

K

R

V

R

V

I

M

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

V

P

F

L

G

G

active site: A73 (≠ N73), G74 (= G74), D319 (≠ M354), Y345 (≠ Q380)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ L275), L245 (= L276), S246 (= S277), G248 (= G279), I249 (≠ L280), D250 (= D281), S251 (= S282), S269 (= S301), M270 (≠ I302), L327 (≠ F362), G344 (= G379), Y345 (≠ Q380), D348 (= D383)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ D358), Y345 (≠ Q380), G346 (= G381), D348 (= D383), I349 (≠ E384)
binding magnesium ion: D250 (= D281), D348 (= D383)

Sites not aligning to the query:

active site: 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440
binding n2-(carboxyethyl)-l-arginine: 354, 370, 379

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
26% identity, 52% coverage: 69:388/618 of query aligns to 65:345/496 of 1mbzA

query
sites
1mbzA

T

T

L

A

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

Y

-

V

-

A

-

L

-

R

R

E

D

Q

E

L

L

I

L

E

S

L

V

G

L

H

P

A

A

F

G

R

P

S

A

-

P

H

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

R

E

Q

R

W

Y

G

D

M

L

E

H

C

A

V

F

T

R

R

L

F

V

E

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

D

G

H

D

Q

R

H

-

Q

-

L

V

M

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

A

C

P

V

V

A

E

P

G

G

G

E

V

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

A

A

A

A

H

G

G

G

F

V

P

N

L

I

A

D

D

L

A

D

R

P

R

V

V

G

A

L

-

H

-

Q

-

F

-

T

-

L

-

H

-

G

-

V

-

P

-

A

-

P

-

H

-

T

-

V

G

L

L

K

T

G

G

V

V

R

Y

K

Q

L

V

A

P

P

A

G

G

Q

A

W

V

M

M

T

D

V

I

N

D

-

L

-

G

P

S

D

G

G

T

Q

A

M

V

Y

T

Q

H

R

R

S

T

W

W

W

T

H

P

L

G

K

L

A

S

E

R

R

R

P

I

G

L

P

P

D

E

H

G

P

E

S

A

S

-

R

-

L

-

Q

-

A

-

L

-

S

-

G

-

V

-

T

-

D

-

H

-

Q

-

A

-

W

-

V

V

E

A

A

A

V

V

H

R

D

A

T

A

L

L

K

E

E

K

A

A

V

V

H

A

K

Q

R

R

L

V

T

T

A

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

I

V

V

A

A

A

L

C

L

A

D

H

E

R

A

A

G

A

V

G

E

E

D

-

I

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

-

E

-

D

-

V

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

D

R

Y

E

S

A

D

R

Q

A

V

V

E

D

R

H

F

L

Q

R

T

T

R

R

H

H

Q

R

K

E

F

I

L

T

I

I

P

P

N

T

E

T

A

E

V

L

L

L

P

A

R

Q

L

L

Q

P

E

Y

A

A

V

V

D

W

A

A

M

S

S

E

-

S

-

V

E

D

P

P

M
x
D

F

I

A

I

Q

E

D
x
Y

A

L

V

L

A

P

F
x
L

Y

T

L

A

L

L

S

Y

E

R

Q

A

V

L

S

D

K

G

E

P

V

E

K

R

V

R

V

I

M

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

V

P

F

L

G

G

active site: A69 (≠ N73), G70 (= G74), D311 (≠ M354), Y337 (≠ Q380)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ L275), L237 (= L276), S238 (= S277), S243 (= S282), S261 (= S301), M262 (≠ I302), Y315 (≠ D358), L319 (≠ F362), G336 (= G379), Y337 (≠ Q380), G338 (= G381), D340 (= D383), I341 (≠ E384)
binding magnesium ion: D242 (= D281), D340 (= D383)
binding pyrophosphate 2-: S238 (= S277), G240 (= G279), D242 (= D281), S243 (= S282), D340 (= D383)

Sites not aligning to the query:

active site: 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 362, 371, 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
27% identity, 52% coverage: 69:388/618 of query aligns to 61:340/491 of 1mc1A

query
sites
1mc1A

T

T

L

A

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

Y

-

V

-

A

-

L

-

R

R

E

D

Q

E

L

L

I

L

E

S

L

V

G

L

H

P

A

A

F

G

R

P

S

A

-

P

H

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

R

E

Q

R

W

Y

G

D

M

L

E

H

C

A

V

F

T

R

R

L

F

V

E

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

D

-

H

-

Q

G

H

D

Q

R

L

V

M

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

A

C

P

V

V

A

E

P

G

G

G

E

V

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

-

A

A

G

G

G

F

V

P

N

L

I

A

D

D

L

A

D

R

P

R

V

V

G

A

L

-

H

-

Q

-

F

-

T

-

L

-

H

-

G

-

V

-

P

-

A

-

P

-

H

-

T

-

V

G

L

L

K

T

G

G

V

V

R

Y

K

Q

L

V

A

P

P

A

G

G

Q

A

W

V

M

M

T

D

V

I

N

D

-

L

-

G

P

S

D

G

G

T

Q

A

M

V

Y

T

Q

H

R

R

S

T

W

W

W

T

H

P

L

G

K

L

A

S

E

R

R

R

P

I

G

L

P

P

D

E

H

G

P

E

S

A

S

-

R

-

L

-

Q

-

A

-

L

-

S

-

G

-

V

-

T

-

D

-

H

-

Q

-

A

-

W

-

V

V

E

A

A

A

V

V

H

R

D

A

T

A

L

L

K

E

E

K

A

A

V

V

H

A

K

Q

R

R

L

V

T

T

A

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

I

V

V

A

A

A

L

C

L

A

D

H

E

R

A

A

G

A

V

G

E

E

D

-

I

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

-

E

-

D

-

V

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

D

R

Y

E

S

A

D

R

Q

A

V

V

E

D

R

H

F

L

Q

R

T

T

R

R

H

H

Q

R

K

E

F

I

L

T

I

I

P

P

N

T

E

T

A

E

V

L

L

L

P

A

R

Q

L

L

Q

P

E

Y

A

A

V

V

D

W

A

A

M

S

S

E

-

S

-

V

E

D

P

P

M
x
D

F

I

A

I

Q

E

D
x
Y

A

L

V

L

A

P

F

L

Y

T

L

A

L

L

S

Y

E

R

Q

A

V

L

S

D

K

G

E

P

V

E

K

R

V

R

V

I

M

L

S

T

G
|
G

Q
x
Y

G
|
G

A

A

D
|
D

E
x
I

V

P

F

L

G

G

active site: A65 (≠ N73), G66 (= G74), D306 (≠ M354), Y332 (≠ Q380)
binding adenosine monophosphate: V231 (≠ L275), S233 (= S277), S238 (= S282), S256 (= S301), M257 (≠ I302), G331 (= G379)
binding magnesium ion: D237 (= D281), D335 (= D383)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ D358), Y332 (≠ Q380), G333 (= G381), I336 (≠ E384)
binding pyrophosphate 2-: S233 (= S277), G235 (= G279), D237 (= D281), S238 (= S282), D335 (= D383)

Sites not aligning to the query:

active site: 366, 427
binding adenosine monophosphate: 427, 430
binding deoxyguanidinoproclavaminic acid: 357, 366, 427
binding pyrophosphate 2-: 407, 427, 428

1mb9A Beta-lactam synthetase complexed with atp (see paper)
26% identity, 52% coverage: 69:388/618 of query aligns to 66:344/485 of 1mb9A

query
sites
1mb9A

T

T

L

A

V

V

F

L

N
x
A

G
|
G

C

E

I

I

Y

Y

N

N

Y

-

V

-

A

-

L

-

R

R

E

D

Q

E

L

L

I

L

E

S

L

V

G

L

H

P

A

A

F

G

R

P

S

A

-

P

H

E

S

G

D

D

T

A

E

E

V

L

I

V

L

L

K

R

A

L

Y

L

R

E

Q

R

W

Y

G

D

M

L

E

H

C

A

V

F

T

R

R

L

F

V

E

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

D

-

H

-

Q

G

H

D

Q

R

L

V

M

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

I

S

K

V

P

P

L

L

Y

Y

Y

T

A

C

P

V

V

A

E

P

G

G

G

E

V

V

R

R

F

A

A

S

S

T

N

E

T

A

Q

K

A

A

L

L

L

A

A

A

A

-

G

-

V

-

N

-

I

-

D

-

L

-

D

-

P

-

V

F

G

P

L

L

H

A

H

D

Q

A

F

R

T

R

L

V

H

A

G

G

V

-

P

-

A

-

P

-

H

-

T

-

V

-

L

L

K

T

G

G

V

V

R

Y

K

Q

L

V

A

P

P

A

G

G

Q

A

W

V

M

M

T

D

V

I

N

D

-

L

-

G

P

S

D

G

G

T

Q

A

M

V

Y

T

Q

H

R

R

S

T

W

W

W

T

H

P

L

G

K

L

A

S

E

R

R

R

P

I

G

L

P

P

D

E

H

G

P

E

S

A

S

-

R

-

L

-

Q

-

A

-

L

-

S

-

G

-

V

-

T

-

D

-

H

-

Q

-

A

-

W

-

V

V

E

A

A

A

V

V

H

R

D

A

T

A

L

L

K

E

E

K

A

A

V

V

H

A

K

Q

R

R

L

V

T

T

A

P

S

G

D

D

V

T

P

P

V

L

G

-

V

V

L
x
V

L
|
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

L

G

I

V

V

A

A

A

L

C

L

A

D

H

E

R

A

A

G

A

V

G

E

E

D

-

I

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

-

E

-

D

-

V

-

P

-

E

T

E

D

K

T

G

S

S

N

E

E

F

F

D

R

Y

E

S

A

D

R

Q

A

V

V

E

D

R

H

F

L

Q

R

T

T

R

R

H

H

Q

R

K

E

F

I

L

T

I

I

P

P

N

T

E

T

A

E

V

L

L

L

P

A

R

Q

L

L

Q

P

E

Y

A

A

V

V

D

W

A

A

M

S

S

E

-

S

-

V

E

D

P

P

M
x
D

F

I

A

I

Q

E

D
x
Y

A

L

V

L

A

P

F
x
L

Y

T

L

A

L

L

S

Y

E

R

Q

A

V

L

S

D

K

G

E

P

V

E

K

R

V

R

V

I

M

L

S

T

G
|
G

Q
x
Y

G

G

A

A

D
|
D

E

I

V

P

F

L

G

G

active site: A70 (≠ N73), G71 (= G74), D310 (≠ M354), Y336 (≠ Q380)
binding adenosine monophosphate: V235 (≠ L275), L236 (= L276), S242 (= S282), S260 (= S301), M261 (≠ I302), Y314 (≠ D358), L318 (≠ F362), G335 (= G379), Y336 (≠ Q380)
binding adenosine-5'-triphosphate: V235 (≠ L275), L236 (= L276), S237 (= S277), G239 (= G279), D241 (= D281), S242 (= S282), S260 (= S301), M261 (≠ I302), L318 (≠ F362), G335 (= G379), D339 (= D383)
binding magnesium ion: D241 (= D281), D339 (= D383)
binding pyrophosphate 2-: S237 (= S277), G239 (= G279), D241 (= D281), S242 (= S282), D339 (= D383)

Sites not aligning to the query:

active site: 370, 431
binding adenosine-5'-triphosphate: 411, 431
binding pyrophosphate 2-: 411, 431

1q19A Carbapenam synthetase (see paper)
24% identity, 61% coverage: 60:436/618 of query aligns to 42:416/500 of 1q19A

query
sites
1q19A

H

Y

Q

Q

P

K

M

C

V

E

D

M

E

E

E

R

L

G

T

T

L

A

V

Y

F

L

N

I

G
|
G

C

S

I

L

Y

Y

N

N

Y

-

V

-

A

-

L

-

R

R

E

T

Q

F

L

L

I

I

E

G

L

L

-

A

-

G

-

V

-

W

-

E

G

G

H

E

A

A

F

Y

R

L

S

A

H

N

S

-

D

D

T

A

E

E

V

L

I

L

L

A

K

L

A

L

Y

F

R

T

Q

R

W

L

G

G

M

A

E

N

C

A

V

L

T

A

R

L

F

A

E

E

G

G

M

D

F

F

A

C

F

F

A

F

L

I

W

-

D

D

D

E

H

P

Q

N

H

G

Q

E

L

L

M

T

L

V

A

I

R

T

D

E

R

S

F

R

G

G

I

F

K

S

P

P

L

V

Y

H

Y

V

A

-

P

-

V

V

E

Q

G

G

G

K

V

K

R

A

F

W

A

M

S

T

N

N

T

S

Q

L

A

K

L

L

L

V

A

T

A

A

-

A

-

E

G

G

G

E

V

G

N

A

I

L

D

W

L

F

D

E

P

E

V

E

G

A

L

L

H

V

H

C

Q

Q

F

S

T

L

L

M

H

R

G

A

V

D

V

T

P

Y

A

T

P

P

H

-

T

-

V

-

L

V

K

K

G

N

V

A

R

Q

K

R

L

L

A

K

P

P

G

G

Q

A

W

V

M

H

T

V

V

L

N

T

P

H

D

D

G

S

Q

E

M

G

Y

Y

Q

S

R

-

S

-

W

-

H

-

L

F

K

V

A

E

E

S

R

R

P

T

G

L

P

T

D

T

H

P

P

A

S

S

S

N

R

Q

L

L

Q

L

A

A

L

L

S

P

G

-

V

-

T

-

D

-

H

-

Q

R

A

E

W

P

V

L

E

L

A

A

V

L

H

I

D

D

T

R

L

Y

K

L

E

N

A

A

V

P

H

L

K

E

R

D

L

L

T

A

A

P

S

R

D

F

V

D

P

T

V

V

G

G

V

I

L
x
P

L
|
L

S
|
S

G

G

L

L

D
|
D

S
|
S

S

S

L

L

I

V

V

T

A

A

L

L

L

A

D

S

E

R

A

H

G

-

V

F

E

K

D

K

I

L

R

N

T

T

F

Y

S
|
S

I
|
I

G

G

F

-

E

-

D

-

V

-

P

-

E

T

E

E

K

L

G

S

S

N

E

E

F

F

D

E

Y

F

S

S

D

Q

Q

Q

V

V

V

A

E

D

R

A

F

L

Q

G

T

T

R

H

H

H

Q

Q

K

M

F

K

L

I

I

L

P

S

N

E

E

T

A

E

V

V

L

I

P

N

R

G

L

I

Q

I

E

E

A

S

V

I

-

Y

D

N

A

E

M

I

S

F

E

D

P

G

M
x
L

F

S

A

A

Q
x
E

D

I

A

Q

V

S

A

G

F

L

Y

F

L

N

L

V

S

Y

E

R

Q

Q

V

A

S

Q

K

G

E

Q

V

V

K

S

V

C

V

M

M

L

S
x
T

G
|
G

Q
x
Y

G
|
G

A

S

D
|
D

E
x
L

V

L

F

F

G

G

Y

I

F

L

-

K

-

P

-

G

-

A

W

Q

Y

Y

P

D

Q

N

M

P

A

N

Q

Q

-

L

A

L

A

A

E

E

K

Q

L

V

-

Y

-
x
R

-

T

-

R

-

W

-

T

-

G

-
x
E

-

F

-

A

-

T

-

H

G

G

P

A

E

S

A

C

Q

Y

A

G

V

I

D

D

A

I

F

R

A

H

P

P

F

F

Y

W

F

-

D

S

R

H

D

S

H

L

A

I

E

S

W

L

S

C

E

H

M

A

I

L

H

H

P

P

D

D

Y

Y

H

K

I

I

R

F

D

D

active site: G56 (= G74), L318 (≠ M354), E321 (≠ Q357), Y344 (≠ Q380), E379 (vs. gap)
binding diphosphomethylphosphonic acid adenosyl ester: P243 (≠ L275), L244 (= L276), S245 (= S277), D249 (= D281), S250 (= S282), S268 (= S301), I269 (= I302), T342 (≠ S378), G343 (= G379), D347 (= D383)
binding (2s,5s)-5-carboxymethylproline: Y344 (≠ Q380), G345 (= G381), L348 (≠ E384), R373 (vs. gap), E379 (vs. gap)

Sites not aligning to the query:

active site: 442
binding diphosphomethylphosphonic acid adenosyl ester: 442, 443, 444, 445

Q9XB61 Carbapenam-3-carboxylate synthase; Carbapenam-3-carboxylate ligase; EC 6.3.3.6 from Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora) (see 3 papers)
24% identity, 61% coverage: 60:436/618 of query aligns to 43:417/503 of Q9XB61

query
sites
Q9XB61

H

Y

Q

Q

P

K

M

C

V

E

D

M

E

E

E

R

L

G

T

T

L

A

V

Y

F

L

N

I

G

G

C

S

I

L

Y

Y

N

N

Y

-

V

-

A

-

L

-

R

R

E

T

Q

F

L

L

I

I

E

G

L

L

-

A

-

G

-

V

-

W

-

E

G

G

H

E

A

A

F

Y

R

L

S

A

H

N

S

-

D

D

T

A

E

E

V

L

I

L

L

A

K

L

A

L

Y

F

R

T

Q

R

W

L

G

G

M

A

E

N

C

A

V

L

T

A

R

L

F

A

E

E

G

G

M

D

F

F

A

C

F

F

A

F

L

I

W

-

D

D

D

E

H

P

Q

N

H

G

Q

E

L

L

M

T

L

V

A

I

R

T

D

E

R

S

F

R

G

G

I

F

K

S

P

P

L

V

Y

H

Y

V

A

-

P

-

V

V

E

Q

G

G

G

K

V

K

R

A

F

W

A

M

S

T

N

N

T

S

Q

L

A

K

L

L

L

V

A

T

A

A

-

A

-

E

G

G

G

E

V

G

N

A

I

L

D

W

L

F

D

E

P

E

V

E

G

A

L

L

H

V

H

C

Q

Q

F

S

T

L

L

M

H

R

G

A

V

D

V

T

P

Y

A

T

P

P

H

-

T

-

V

-

L

V

K

K

G

N

V

A

R

Q

K

R

L

L

A

K

P

P

G

G

Q

A

W

V

M

H

T

V

V

L

N

T

P

H

D

D

G

S

Q

E

M

G

Y

Y

Q

S

R

-

S

-

W

-

H

-

L

F

K

V

A

E

E

S

R

R

P

T

G

L

P

T

D

T

H

P

P

A

S

S

S

N

R

Q

L

L

Q

L

A

A

L

L

S

P

G

-

V

-

T

-

D

-

H

-

Q

R

A

E

W

P

V

L

E

L

A

A

V

L

H

I

D

D

T

R

L

Y

K

L

E

N

A

A

V

P

H

L

K

E

R

D

L

L

T

A

A

P

S

R

D

F

V

D

P

T

V

V

G

G

V

I

L
x
P

L
|
L

S
|
S

G
|
G

L
|
L

D
|
D

S
|
S

S

S

L

L

I

V

V

T

A

A

L

L

L

A

D

S

E

R

A

H

G

-

V

F

E

K

D

K

I

L

R

N

T

T

F

Y

S

S

I
|
I

G

G

F

-

E

-

D

-

V

-

P

-

E

T

E

E

K

L

G

S

S

N

E

E

F

F

D

E

Y

F

S

S

D

Q

Q

Q

V

V

V

A

E

D

R

A

F

L

Q

G

T

T

R

H

H

H

Q

Q

K

M

F

K

L

I

I

L

P

S

N

E

E

T

A

E

V

V

L

I

P

N

R

G

L

I

Q

I

E

E

A

S

V

I

-

Y

D

N

A

E

M

I

S

F

E

D

P

G

M

L

F

S

A

A

Q

E

D

I

A

Q

V

S

A

G

F

L

Y

F

L

N

L

V

S

Y

E

R

Q

Q

V

A

S

Q

K

G

E

Q

V

V

K

S

V

C

V

M

M

L

S

T

G
|
G

Q
x
Y

G
|
G

A
x
S

D
|
D

E

L

V

L

F

F

G

G

Y

I

F

L

-

K

-

P

-

G

-

A

W

Q

Y

Y

P

D

Q

N

M

P

A

N

Q

Q

-

L

A

L

A

A

E

E

K
x
Q

L

V

-

Y

-
x
R

-

T

-

R

-

W

-

T

-

G

-
x
E

-

F

-

A

-

T

-

H

G

G

P

A

E

S

A

C

Q

Y

A

G

V

I

D

D

A

I

F

R

A

H

P

P

F

F

Y

W

F

-

D

S

R

H

D

S

H

L

A

I

E

S

W

L

S

C

E

H

M

A

I

L

H

H

P

P

D

D

Y

Y

H

K

I

I

R

F

D

D

244:251 (vs. 275:282, 88% identical) binding ATP
I270 (= I302) binding ATP
GYGSD 344:348 (≠ GQGAD 379:383) binding ATP
Y345 (≠ Q380) mutation to A: Loss of activity.; mutation to F: Reduces catalytic efficiency.
G346 (= G381) binding substrate
Q371 (≠ K401) binding substrate
R374 (vs. gap) binding substrate
E380 (vs. gap) mutation to A: Loss of activity.; mutation to D: Reduces catalytic efficiency.; mutation to Q: Reduces catalytic efficiency.

Sites not aligning to the query:

421 binding ATP
443 mutation K->A,M: Loss of activity.
444:446 binding ATP

2j6hA E. Coli glucosamine-6-p synthase in complex with glucose-6p and 5-oxo- l-norleucine (see paper)
27% identity, 26% coverage: 2:164/618 of query aligns to 1:197/608 of 2j6hA

query
sites
2j6hA

C
|
C

G

G

I

I

C

V

G

G

E

A

I

I

Y

-

W

-

D

-

G

A

Q

Q

K

R

A

D

S

V

E

A

A

E

T

I

L

L

A

L

P

E

M

G

L

L

E

R

K

R

L

L

A

E

K

Y

R
|
R

G
|
G

P

Y

D

D

D

S

G

A

G

G

I

L

W

A

-

V

-

D

-

A

-

E

-

G

-

H

-

M

-

T

-

R

-

L

-

R

-

L

-

G

-

K

-

V

-

Q

-

M

-

L

-

A

-

Q

-

A

-

E

-

H

-

P

L

L

Q

H

N

G

Q

G

V

T

G

G

L

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

-

G

D

E

L

P

S

S

D

E

A

V

G

N

H

A

Q
x
H

P

P

M

H

V

V

D

S

E

E

E

H

L

I

T

V

L

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

Y

H

V

E

A

P

L

L

R

R

E

E

Q

E

L

L

I

K

E

A

L

R

G

G

H

Y

A

T

F

F

R

V

S

S

H

E

S

T

D
|
D

T

T

E

E

V

V

I

I

-

A

-

H

-

L

-

V

-

N

-

W

-

E

L

L

K

K

-

Q

-

G

A

T

Y

L

R

R

Q

E

W

A

G

V

M

L

E

R

C

A

V

I

T

P

R

Q

F

L

E

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

D

S

H

R

Q

H

H

P

Q

D

L

T

M

L

L

L

A

A

R

A

D

-

R

R

F

S

G

G

I

-

K

S

P

P

L

L

Y

V

Y

I

A

G

P

L

V

G

E

M

G

G

G

E

V

N

R

F

F

I

A

A

S

S

N

D

T

Q

Q

L

A

A

L

L

active site: C1 (= C2), R26 (= R30), G27 (= G31), W74 (≠ L50), N98 (= N73), G99 (= G74)
binding 5-oxo-l-norleucine: C1 (= C2), R73 (= R49), W74 (≠ L50), T76 (≠ I52), H86 (≠ Q61), N98 (= N73), G99 (= G74), D123 (= D98)

Sites not aligning to the query:

active site: 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 347, 348, 349, 352, 399, 401, 488

4amvA E.Coli glucosamine-6p synthase in complex with fructose-6p (see paper)
27% identity, 26% coverage: 2:164/618 of query aligns to 1:197/608 of 4amvA

query
sites
4amvA

C
|
C

G

G

I

I

C

V

G

G

E

A

I

I

Y

-

W

-

D

-

G

A

Q

Q

K

R

A

D

S

V

E

A

A

E

T

I

L

L

A

L

P

E

M

G

L

L

E

R

K

R

L

L

A

E

K

Y

R
|
R

G
|
G

P

Y

D

D

D

S

G

A

G

G

I

L

W

A

-

V

-

D

-

A

-

E

-

G

-

H

-

M

-

T

-

R

-

L

-

R

-

L

-

G

-

K

-

V

-

Q

-

M

-

L

-

A

-

Q

-

A

-

E

-

H

-

P

L

L

Q

H

N

G

Q

G

V

T

G

G

L

I

G

A

H

H

R

T

R

R

L
x
W

S

A

I

T

I

H

-

G

D

E

L

P

S

S

D

E

A

V

G

N

H

A

Q

H

P

P

M

H

V

V

D

S

E

E

E

H

L

I

T

V

L

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

Y

H

V

E

A

P

L

L

R

R

E

E

Q

E

L

L

I

K

E

A

L

R

G

G

H

Y

A

T

F

F

R

V

S

S

H

E

S

T

D

D

T

T

E

E

V

V

I

I

-

A

-

H

-

L

-

V

-

N

-

W

-

E

L

L

K

K

-

Q

-

G

A

T

Y

L

R

R

Q

E

W

A

G

V

M

L

E

R

C

A

V

I

T

P

R

Q

F

L

E

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

D

S

-

R

H

H

Q

P

H

D

Q

T

L

L

M

L

L

A

A

A

R

R

D

S

R

G

F

-

G

-

I

-

K

S

P

P

L

L

Y

V

Y

I

A

G

P

L

V

G

E

M

G

G

G

E

V

N

R

F

F

I

A

A

S

S

N

D

T

Q

Q

L

A

A

L

L

active site: C1 (= C2), R26 (= R30), G27 (= G31), W74 (≠ L50), N98 (= N73), G99 (= G74)

Sites not aligning to the query:

active site: 248, 481, 485, 488, 504, 603
binding fructose -6-phosphate: 301, 302, 303, 347, 348, 349, 352, 401, 485, 488

1jxaA Glucosamine 6-phosphate synthase with glucose 6-phosphate (see paper)
27% identity, 26% coverage: 2:164/618 of query aligns to 1:197/608 of 1jxaA

query
sites
1jxaA

C
|
C

G

G

I

I

C

V

G

G

E

A

I

I

Y

-

W

-

D

-

G

A

Q

Q

K

R

A

D

S

V

E

A

A

E

T

I

L

L

A

L

P

E

M

G

L

L

E

R

K

R

L

L

A

E

K

Y

R
|
R

G
|
G

P

Y

D

D

D

S

G

A

G

G

I

L

W

A

-

V

-

D

-

A

-

E

-

G

-

H

-

M

-

T

-

R

-

L

-

R

-

L

-

G

-

K

-

V

-

Q

-

M

-

L

-

A

-

Q

-

A

-

E

-

H

-

P

L

L

Q

H

N

G

Q

G

V

T

G

G

L

I

G

A

H

H

R

T

R

R

L
x
W

S

A

I

T

I

H

-

G

D

E

L

P

S

S

D

E

A

V

G

N

H

A

Q

H

P

P

M

H

V

V

D

S

E

E

E

H

L

I

T

V

L

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

Y

H

V

E

A

P

L

L

R

R

E

E

Q

E

L

L

I

K

E

A

L

R

G

G

H

Y

A

T

F

F

R

V

S

S

H

E

S

T

D

D

T

T

E

E

V

V

I

I

-

A

-

H

-

L

-

V

-

N

-

W

-

E

L

L

K

K

-

Q

-

G

A

T

Y

L

R

R

Q

E

W

A

G

V

M

L

E

R

C

A

V

I

T

P

R

Q

F

L

E

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

D

S

-

R

H

H

Q

P

H

D

Q

T

L

L

M

L

L

A

A

A

R

R

D

S

R

G

F

-

G

-

I

-

K

S

P

P

L

L

Y

V

Y

I

A

G

P

L

V

G

E

M

G

G

G

E

V

N

R

F

F

I

A

A

S

S

N

D

T

Q

Q

L

A

A

L

L

active site: C1 (= C2), R26 (= R30), G27 (= G31), W74 (≠ L50), N98 (= N73), G99 (= G74)

Sites not aligning to the query:

active site: 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 303, 347, 348, 349, 352, 401, 485, 488

1xfgA Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate (see paper)
27% identity, 26% coverage: 2:164/618 of query aligns to 1:197/238 of 1xfgA

query
sites
1xfgA

C
|
C

G

G

I

I

C

V

G

G

E

A

I

I

Y

-

W

-

D

-

G

A

Q

Q

K

R

A

D

S

V

E

A

A

E

T

I

L

L

A

L

P

E

M

G

L

L

E

R

K

R

L

L

A

E

K

Y

R
|
R

G
|
G

P

Y

D

D

D

S

G

A

G

G

I

L

W

A

-

V

-

D

-

A

-

E

-

G

-

H

-

M

-

T

-

R

-

L

-

R

-

L

-

G

-

K

-

V

-

Q

-

M

-

L

-

A

-

Q

-

A

-

E

-

H

-

P

L

L

Q

H

N

G

Q

G

V

T

G

G

L

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

-

G

D

E

L

P

S

S

D

E

A

V

G

N

H

A

Q
x
H

P

P

M

H

V

V

D

S

E

E

E

H

L

I

T

V

L

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

Y

H

V

E

A

P

L

L

R

R

E

E

Q

E

L

L

I

K

E

A

L

R

G

G

H

Y

A

T

F

F

R

V

S

S

H

E

S

T

D
|
D

T

T

E

E

V

V

I

I

-

A

-

H

-

L

-

V

-

N

-

W

-

E

L

L

K

K

-

Q

-

G

A

T

Y

L

R

R

Q

E

W

A

G

V

M

L

E

R

C

A

V

I

T

P

R

Q

F

L

E

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

D

S

H

R

Q

H

H

P

Q

D

L

T

M

L

L

L

A

A

R

-

D

A

R

R

F

S

G

G

I

-

K

S

P

P

L

L

Y

V

Y

I

A

G

P

L

V

G

E

M

G

G

G

E

V

N

R

F

F

I

A

A

S

S

N

D

T

Q

Q

L

A

A

L

L

active site: C1 (= C2), R26 (= R30), G27 (= G31), W74 (≠ L50), N98 (= N73), G99 (= G74)
binding glutamine hydroxamate: C1 (= C2), R73 (= R49), W74 (≠ L50), T76 (≠ I52), H86 (≠ Q61), N98 (= N73), G99 (= G74), D123 (= D98)

1xffA Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate (see paper)
27% identity, 26% coverage: 2:164/618 of query aligns to 1:197/238 of 1xffA

query
sites
1xffA

C
|
C

G

G

I

I

C

V

G

G

E

A

I

I

Y

-

W

-

D

-

G

A

Q

Q

K

R

A

D

S

V

E

A

A

E

T

I

L

L

A

L

P

E

M

G

L

L

E

R

K

R

L

L

A

E

K

Y

R
|
R

G
|
G

P

Y

D

D

D

S

G

A

G

G

I

L

W

A

-

V

-

D

-

A

-

E

-

G

-

H

-

M

-

T

-

R

-

L

-

R

-

L

-

G

-

K

-

V

-

Q

-

M

-

L

-

A

-

Q

-

A

-

E

-

H

-

P

L

L

Q

H

N

G

Q

G

V

T

G

G

L

I

G

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

-

G

D

E

L

P

S

S

D

E

A

V

G

N

H

A

Q
x
H

P

P

M

H

V

V

D

S

E

E

E

H

L

I

T

V

L

V

V

V

F

H

N
|
N

G
|
G

C

I

I

I

Y

E

N

N

Y

H

V

E

A

P

L

L

R

R

E

E

Q

E

L

L

I

K

E

A

L

R

G

G

H

Y

A

T

F

F

R

V

S

S

H

E

S

T

D
|
D

T

T

E

E

V

V

I

I

-

A

-

H

-

L

-

V

-

N

-

W

-

E

L

L

K

K

-

Q

-

G

A

T

Y

L

R

R

Q

E

W

A

G

V

M

L

E

R

C

A

V

I

T

P

R

Q

F

L

E

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

D

S

H

R

Q

H

H

P

Q

D

L

T

M

L

L

L

A

A

R

-

D

A

R

R

F

S

G

G

I

-

K

S

P

P

L

L

Y

V

Y

I

A

G

P

L

V

G

E

M

G

G

G

E

V

N

R

F

F

I

A

A

S

S

N

D

T

Q

Q

L

A

A

L

L

active site: C1 (= C2), R26 (= R30), G27 (= G31), W74 (≠ L50), N98 (= N73), G99 (= G74)
binding glutamic acid: C1 (= C2), R73 (= R49), W74 (≠ L50), T76 (≠ I52), H86 (≠ Q61), N98 (= N73), G99 (= G74), D123 (= D98)

Query Sequence

>WP_019894569.1 NCBI__GCF_000384235.1:WP_019894569.1
MCGICGEIYWDGQKASEATLAPMLEKLAKRGPDDGGIWLQNQVGLGHRRLSIIDLSDAGH
QPMVDEELTLVFNGCIYNYVALREQLIELGHAFRSHSDTEVILKAYRQWGMECVTRFEGM
FAFALWDDHQHQLMLARDRFGIKPLYYAPVEGGVRFASNTQALLAAGGVNIDLDPVGLHH
QFTLHGVVPAPHTVLKGVRKLAPGQWMTVNPDGQMYQRSWWHLKAERPGPDHPSSRLQAL
SGVTDHQAWVEAVHDTLKEAVHKRLTASDVPVGVLLSGGLDSSLIVALLDEAGVEDIRTF
SIGFEDVPEEKGSEFDYSDQVVERFQTRHQKFLIPNEAVLPRLQEAVDAMSEPMFAQDAV
AFYLLSEQVSKEVKVVMSGQGADEVFGGYFWYPQMAQAAEKLGPEAQAVDAFAPFYFDRD
HAEWSEMIHPDYHIRDVTTEWVQDRLSEEDADTFIDQVLRLDASHLIVDDPVKRVDNMTM
AWGLEARVPFLDHSLVEVAMTAPPETKLQDFKYLLKAVARGRVPDSVIDRPKGYFPMPAL
KYVRGPFYDMMRSVLTSEVAQKRGLFQSDYIERLLAEPEADASFTRIQGSKLWHAALLEL
WLQTHVDPYRERNLCSAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory