SitesBLAST
Comparing WP_019895362.1 NCBI__GCF_000384235.1:WP_019895362.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6rl5G The first crystal structure of the daba aminotransferase ectb in the ectoine biosynthesis pathway of the model organism chromohalobacter salexigens dsm 3034 (see paper)
49% identity, 98% coverage: 4:411/417 of query aligns to 5:415/422 of 6rl5G
- active site: S16 (= S15), F137 (= F136), D237 (= D235), K266 (= K264)
- binding pyridoxal-5'-phosphate: G110 (= G109), T111 (= T110), F137 (= F136), H138 (= H137), D237 (= D235), I239 (= I237), Q240 (= Q238), K266 (= K264), G294 (= G293), T295 (= T294)
1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 94% coverage: 21:412/417 of query aligns to 22:387/387 of 1wkhA
- active site: F132 (= F136), E184 (= E202), D217 (= D235), Q220 (= Q238), K246 (= K264), T275 (= T294), R363 (≠ K388)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ A45), S104 (≠ T108), G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), E184 (= E202), E189 (= E207), D217 (= D235), I219 (= I237), K246 (= K264), R363 (≠ K388)
Sites not aligning to the query:
1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 94% coverage: 21:412/417 of query aligns to 22:387/387 of 1wkgA
- active site: F132 (= F136), E184 (= E202), D217 (= D235), Q220 (= Q238), K246 (= K264), T275 (= T294), R363 (≠ K388)
- binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ A45), G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), R135 (≠ M139), E184 (= E202), D217 (= D235), I219 (= I237), Q220 (= Q238), K246 (= K264), G273 (≠ T292), T274 (≠ G293), T275 (= T294)
Sites not aligning to the query:
1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 94% coverage: 21:412/417 of query aligns to 22:387/387 of 1vefA
- active site: F132 (= F136), D217 (= D235), K246 (= K264), T275 (= T294), R363 (≠ K388)
- binding pyridoxal-5'-phosphate: G105 (= G109), T106 (= T110), F132 (= F136), S133 (≠ H137), E184 (= E202), D217 (= D235), I219 (= I237), K246 (= K264)
Sites not aligning to the query:
Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
34% identity, 99% coverage: 2:412/417 of query aligns to 8:395/395 of Q5SHH5
- GT 113:114 (= GT 109:110) binding pyridoxal 5'-phosphate
- K254 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T283 (= T294) binding pyridoxal 5'-phosphate
4uoyA Crystal structure of ygjg in complex with pyridoxal-5'-phosphate (see paper)
32% identity, 92% coverage: 32:413/417 of query aligns to 72:445/454 of 4uoyA
- active site: F174 (= F136), E232 (= E202), D265 (= D235), Q268 (= Q238), K294 (= K264), T326 (= T294), R420 (≠ K388)
- binding pyridoxal-5'-phosphate: G144 (= G109), T145 (= T110), F174 (= F136), H175 (= H137), G176 (= G138), E232 (= E202), D265 (= D235), V267 (≠ I237), Q268 (= Q238), K294 (= K264)
Sites not aligning to the query:
4uoxA Crystal structure of ygjg in complex with pyridoxal-5'-phosphate and putrescine (see paper)
32% identity, 92% coverage: 32:413/417 of query aligns to 72:445/453 of 4uoxA
- active site: F174 (= F136), E232 (= E202), D265 (= D235), Q268 (= Q238), K294 (= K264), T326 (= T294), R420 (≠ K388)
- binding pyridoxal-5'-phosphate: S143 (≠ T108), G144 (= G109), T145 (= T110), F174 (= F136), H175 (= H137), G176 (= G138), D265 (= D235), V267 (≠ I237), Q268 (= Q238), T325 (≠ G293), T326 (= T294)
- binding 1,4-diaminobutane: E237 (= E207), K294 (= K264)
Sites not aligning to the query:
8cplC Yzw2 a scaffold for cryo-em of small proteins of interest
32% identity, 92% coverage: 32:413/417 of query aligns to 70:443/499 of 8cplC
P42588 Putrescine aminotransferase; PAT; PATase; Cadaverine transaminase; Diamine transaminase; Putrescine transaminase; Putrescine--2-oxoglutaric acid transaminase; Putrescine:2-OG aminotransferase; EC 2.6.1.82; EC 2.6.1.29 from Escherichia coli (strain K12) (see paper)
32% identity, 92% coverage: 32:413/417 of query aligns to 78:451/459 of P42588
- GT 150:151 (= GT 109:110) binding in other chain
- Q274 (= Q238) binding in other chain
- K300 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T332 (= T294) binding pyridoxal 5'-phosphate
P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
31% identity, 96% coverage: 12:411/417 of query aligns to 17:421/426 of P22256
- I50 (≠ A45) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
- GS 111:112 (≠ GT 109:110) binding pyridoxal 5'-phosphate
- E211 (= E207) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
- V241 (≠ I237) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
- Q242 (= Q238) binding pyridoxal 5'-phosphate
- K268 (= K264) modified: N6-(pyridoxal phosphate)lysine
- T297 (= T294) binding pyridoxal 5'-phosphate
1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
31% identity, 96% coverage: 12:411/417 of query aligns to 16:420/425 of 1sffA
- active site: V18 (≠ Y14), Y137 (≠ F136), E205 (= E202), D238 (= D235), Q241 (= Q238), K267 (= K264), T296 (= T294), R397 (≠ K388)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (≠ M77), G110 (= G109), S111 (≠ T110), Y137 (≠ F136), H138 (= H137), R140 (≠ M139), E205 (= E202), D238 (= D235), V240 (≠ I237), Q241 (= Q238), K267 (= K264), T296 (= T294)
- binding sulfate ion: N152 (≠ G150), Y393 (≠ G384)
1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
31% identity, 96% coverage: 12:411/417 of query aligns to 16:420/425 of 1sf2A