SitesBLAST

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
42% identity, 78% coverage: 43:274/297 of query aligns to 11:259/304 of Q96XT4

query
sites
Q96XT4

W

W

C
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C

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P

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G

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C

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G

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D

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K

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C12 (= C44) binding [4Fe-4S] cluster
C15 (= C47) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 76:79) binding thiamine diphosphate
C46 (= C78) binding [4Fe-4S] cluster
D90 (= D120) binding Mg(2+)
GD 91:92 (= GD 121:122) binding thiamine diphosphate
N118 (= N148) binding Mg(2+)
V120 (= V150) binding Mg(2+)
GL 122:123 (≠ GM 152:153) binding thiamine diphosphate
C197 (= C227) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
42% identity, 78% coverage: 43:274/297 of query aligns to 8:256/301 of 5b46B

query
sites
5b46B

W
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W

C
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C

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P

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G

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C

G

G

H

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F

F

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T

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binding magnesium ion: D87 (= D120), N115 (= N148), V117 (= V150)
binding iron/sulfur cluster: W8 (= W43), C9 (= C44), C12 (= C47), C43 (= C78), C194 (= C227), T196 (= T229), Y197 (≠ F230)
binding thiamine diphosphate: I41 (= I76), G42 (= G77), C43 (= C78), S44 (= S79), H62 (= H95), G86 (= G119), G88 (= G121), D89 (= D122), N115 (= N148), V117 (= V150), Y118 (= Y151), G119 (= G152), L120 (≠ M153), T121 (= T154)

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
40% identity, 78% coverage: 43:274/297 of query aligns to 4:230/275 of 5b47B

query
sites
5b47B

W

W

C
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C

P

P

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G

C
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C

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G

H

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F

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W

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binding magnesium ion: D83 (= D120), N111 (= N148)
binding iron/sulfur cluster: C5 (= C44), C8 (= C47), C39 (= C78), C179 (= C227)
binding thiamine diphosphate: I37 (= I76), G38 (= G77), C39 (= C78), S40 (= S79), H58 (= H95), G84 (= G121), D85 (= D122), N111 (= N148), V113 (= V150), Y114 (= Y151), L116 (≠ M153)

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
39% identity, 78% coverage: 43:274/297 of query aligns to 11:259/305 of Q96Y68

query
sites
Q96Y68

W

W

C
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C

P

P

G

G

C
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C

G

G

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N

F

F

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G

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A

A

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D

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N

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D

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D

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V

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G

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K

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A

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E

C12 (= C44) binding [4Fe-4S] cluster
C15 (= C47) binding [4Fe-4S] cluster
C46 (= C78) binding [4Fe-4S] cluster
K49 (≠ R81) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D120) binding Mg(2+)
GD 91:92 (= GD 121:122) binding thiamine diphosphate
N118 (= N148) binding Mg(2+)
V120 (= V150) binding Mg(2+)
GL 122:123 (≠ GM 152:153) binding thiamine diphosphate
K125 (= K155) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (= K203) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C227) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
39% identity, 78% coverage: 43:274/297 of query aligns to 11:259/305 of P72579

query
sites
P72579

W

W

C

C

P

P

G

G

C

C

G

G

H

N

F

F

F

G

V

I

L

L

R

N

A

A

L

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K

Q

A

A

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A

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G

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C

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x
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P

A

H

Y

F

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A

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F

A

A

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P

P

D

D

L

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G

D

D

G

G

D

D

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I

G

G

G

A

N

G

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H

F

F

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H

A

A

A

C

G

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R

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N

M

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D

D

M

M

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Y

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L

M

H

D

D

N

N

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Y

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G

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x
L

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T

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K

G

G

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K

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G

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N

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A

A

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G

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F

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F

Y

S

A

G

Y

D

D

P

V

K

K

G

H

L

L

T

K

K

E

L

L

I

I

S

K

D

S

G

A

I

I

E

K

H

H

K

K

G

G

F

L

S

A

V

L

I

I

E

D

I

V

K

L

S

Q

P

P

C

C

V

P

T

T

F

Y

R

N

P

D

-

I

E

N

Q

T

K

K

A

E

W

W

-

Y

Q

D

K

K

Q

R

M

I

R

Y

P

K

L

L

N

D

I

T

E

L

P

P

-

D

-

W

-

D

-

P

-

V

-

K

-

P

-

E

T

V

P

N

N

E

K

K

L

I

E

K

A

R

A

A

-

I

-

D

Q

K

A

S

I

L

H

E

A

W

S

G

D

D

G

R

F

I

D

P

T

I

G

G

I

I

F

F

Y

Y

Q

Q

A

N

D

E

K49 (≠ R81) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (≠ M153) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
39% identity, 78% coverage: 43:274/297 of query aligns to 7:241/286 of 5b48B

query
sites
5b48B

W

W

C
|
C

P

P

G

G

C
|
C

G

G

H

N

F

F

F

G

V

I

L

L

R

N

A

A

L

E

T

Q

K

Q

A

A

L

I

A

V

W

E

L

L

N

G

R

V

P

D

P

T

E

K

E

N

L

V

A

V

M

V

I

V

T

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

R

K

L

I

P

P

A

H

Y

F

L

T

S

P

S

I

Y

S

G

G

F

V

H

H

G

T

V

L

H
|
H

G

G

R

R

A

A

L

I

A

A

V

F

A

A

A

T

G

G

L

I

K

K

M

L

A

S

R

N

P

P

D

D

L

L

T

V

V

V

L

I

V

V

A

N

G

G

D
|
D

G
|
G

D
|
D

G

L

Y

L

S

G

I

I

G

G

G

A

N

G

H

H

F

F

L

V

H

A

A

A

C

G

R

R

N

N

M

V

D

D

M

M

T

V

Y

V

L

I

V

L

M

H

D

D

N
|
N

E

G

V
|
V

Y
|
Y

G
|
G

M
x
L

T

T

K

K

G

G

Q

Q

P

A

S

S

P

P

T

T

S

-

D

-

P

-

D

-

W

-

H

-

S

-

K

-

L

-

A

L

P

K

H

R

G

G

T

E

G

N

I

I

T

N

-

D

A

A

F

V

N

N

P

P

L

I

G

A

V

L

A

A

L

I

A

S

G

G

A

Y

S

T

F

F

I

V

A

A

R

R

G

G

F

Y

S

A

G

Y

D

D

P

V

K

K

G

H

L

L

T

K

K

E

L

L

I

I

S

K

D

S

G

A

I

I

E

K

H

H

K

K

G

G

F

L

S

A

V

L

I

I

E

D

I

V

K

L

S

Q

P

P

C
|
C

V

P

T
|
T

F

Y

R

N

P

D

-

I

E

N

Q

T

K

K

A

E

W

W

Q

R

-

I

-

Y

-

K

-

L

-

D

-

T

-

L

K

P

Q

D

M

W

R

D

P

P

L

V

N

V

I

K

E

K

P

P

T

E

P

E

N

V

K

N

L

E

E

K

A

I

A

K

Q

R

A

A

I

I

H

D

A

K

S

S

-

L

-

E

-

W

D

G

G

D

F

I

D

P

T

I

G

G

I

I

F

F

Y

Y

Q

Q

A

N

D

E

binding magnesium ion: D84 (= D120), N112 (= N148), V114 (= V150)
binding iron/sulfur cluster: C8 (= C44), C11 (= C47), C42 (= C78), C183 (= C227), T185 (= T229)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I76), G41 (= G77), C42 (= C78), S43 (= S79), H59 (= H95), G85 (= G121), D86 (= D122), N112 (= N148), V114 (= V150), Y115 (= Y151), G116 (= G152), L117 (≠ M153)

5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
25% identity, 71% coverage: 27:238/297 of query aligns to 8:230/310 of 5exdF

query
sites
5exdF

V

I

R

K

P

K

A

A

K

P

P

D

N

E

D

E

Y

Y

L

Y

S

V

Q

P

N

G

H

H

P

R

V

T

W

-

C
|
C

P

A

G

G

C
|
C

G

G

H
x
P

F

A

F

L

V

T

L

Y

R

R

A

L

L

V

T

A

K

K

A

A

L

A

A

G

W

-

L

-

N

-

R

-

P

-

P

P

E

N

E

T

L

I

A

-

M

F

I

I

T

G

G

P

I
x
T

G
|
G

C
|
C

S

-

R

-

L

M

P

Y

A

V

Y

A

L

N

S

T

S

S

Y

Y

G

G

-

C

-

G

-

P

-

W

-

R

-

V

-

P

-

W

F

I

H

H

G

A

V

Q

H
x
I

G

T

R

N

A

G

L

G

A

A

V

V

A

A

A

S

G

G

L

I

K

E

M

A

A

A

R

Y

P

K

D

A

L

M

-

I

-

R

-

K

-

T

-

D

-

A

-

E

-

F

-

P

T

N

V

I

L

I

V

V

A

M

G

A

G

G

D
|
D

G
|
G

D

G

G

A

Y

V

S

D

I

I

G

G

G

L

N

Q

H

A

F

L

L

S

H

A

A

M

C

L

R

Y

R

R

N

G

M

H

D

D

M

V

T

L

Y

F

L

I

V

C

M
x
Y

D

D

N
|
N

E

E

V
x
S

Y
|
Y

G
x
A

M
x
N

T
|
T

K

G

G

I

Q

Q

P

T

S

S

P

P

T

T

S

T

D

P

P

Y

D

G

W

A

H

N

S

T

K

T

L

F

A

T

P

P

H

P

G

G

-

E

-

V

-

P

T

E

G

G

I

K

T

K

A

L

F

F

-

P

-

K

-

D

N

N

P

P

L

K

G

V

V

I

A

A

L

H

A

G

-

H

A

P

G

E

A

L

S

K

F

Y

I

V

A

A

R

T

G

A

F

S

S

I

G

G

D

W

P

P

K

V

G

D

L

L

T

M

K

N

L

K

I

V

S

R

D

K

G

G

I

L

E

N

H

Q

K

E

G

G

F

P

S

A

V

Y

I

I

E

H

I

I

K

H

S

A

P

P

C
|
C

V

-

T

-

F

-

R

-

P

-

E

-

Q

P

K
|
K

A

G

W

W

Q

Q

active site: N143 (≠ M153)
binding magnesium ion: D110 (= D120), N138 (= N148), S140 (≠ V150)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ I76), G51 (= G77), C52 (= C78), I74 (≠ H95), D110 (= D120), G111 (= G121), Y136 (≠ M146), N138 (= N148), S140 (≠ V150), Y141 (= Y151), A142 (≠ G152), N143 (≠ M153), T144 (= T154)
binding iron/sulfur cluster: C24 (= C44), C27 (= C47), P29 (≠ H49), C52 (= C78), A142 (≠ G152), C225 (= C227), K227 (= K235)

5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
25% identity, 71% coverage: 27:238/297 of query aligns to 8:230/314 of 5exeC

query
sites
5exeC

V

I

R

K

P

K

A

A

K

P

P

D

N

E

D

E

Y

Y

L

Y

S

V

Q

P

N

G

H

H

P

R

V

T

W

-

C
|
C

P

A

G

G

C
|
C

G

G

H
x
P

F

A

F

L

V

T

L

Y

R

R

A

L

L

V

T

A

K

K

A

A

L

A

A

G

W

-

L

-

N

-

R

-

P

-

P

P

E

N

E

T

L

I

A

-

M

F

I

I

T

G

G

P

I
x
T

G
|
G

C
|
C

S

-

R

-

L

M

P

Y

A

V

Y

A

L

N

S

T

S

S

Y

Y

G

G

-

C

-

G

-

P

-

W

-

R

-

V

-

P

-

W

F

I

H

H

G

A

V

Q

H
x
I

G

T

R

N

A

G

L

G

A

A

V

V

A

A

A

S

G

G

L

I

K

E

M

A

A

A

R

Y

P

K

D

A

L

M

-

I

-

R

-

K

-

T

-

D

-

A

-

E

-

F

-

P

T

N

V

I

L

I

V

V

A

M

G

A

G
|
G

D
|
D

G
|
G

D

G

G

A

Y

V

S

D

I

I

G

G

G

L

N

Q

H

A

F

L

L

S

H

A

A

M

C

L

R

Y

R

R

N

G

M

H

D
|
D

M

V

T

L

Y

F

L

I

V

C

M
x
Y

D

D

N
|
N

E

E

V
x
S

Y
|
Y

G
x
A

M
x
N

T
|
T

K

G

G

I

Q

Q

P

T

S

S

P

P

T

T

S

T

D

P

P

Y

D

G

W

A

H

N

S

T

K

T

L

F

A

T

P

P

H

P

G

G

-

E

-

V

-

P

T

E

G

G

I

K

T

K

A

L

F

F

-

P

-

K

-

D

N

N

P

P

L

K

G

V

V

I

A

A

L

H

A

G

-

H

A

P

G

E

A

L

S

K

F

Y

I

V

A

A

R

T

G

A

F

S

S

I

G

G

D

W

P

P

K

V

G

D

L

L

T

M

K

N

L

K

I

V

S

R

D

K

G

G

I
x
L

E

N

H
x
Q

K

E

G

G

F

P

S

A

V

Y

I

I

E

H

I

I

K

H

S

A

P

P

C
|
C

V

-

T

-

F

-

R

-

P

-

E

-

Q
x
P

K
|
K

A

G

W

W

Q

Q

active site: N143 (≠ M153)
binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ I76), G51 (= G77), C52 (= C78), I74 (≠ H95), G109 (= G119), D110 (= D120), G111 (= G121), Y136 (≠ M146), N138 (= N148), S140 (≠ V150), Y141 (= Y151), A142 (≠ G152), N143 (≠ M153), T144 (= T154)
binding magnesium ion: D110 (= D120), D130 (= D140), N138 (= N148), S140 (≠ V150), L211 (≠ I213), Q213 (≠ H215)
binding iron/sulfur cluster: C24 (= C44), C27 (= C47), P29 (≠ H49), C52 (= C78), C225 (= C227), P226 (≠ Q234), K227 (= K235)

5c4iC Structure of an oxalate oxidoreductase (see paper)
25% identity, 71% coverage: 27:238/297 of query aligns to 8:230/312 of 5c4iC

query
sites
5c4iC

V

I

R

K

P

K

A

A

K

P

P

D

N

E

D

E

Y

Y

L

Y

S

V

Q

P

N

G

H

H

P

R

V

T

W

-

C
|
C

P

A

G

G

C
|
C

G

G

H
x
P

F

A

F

L

V

T

L

Y

R

R

A

L

L

V

T

A

K

K

A

A

L

A

A

G

W

-

L

-

N

-

R

-

P

-

P

P

E

N

E

T

L

I

A

-

M

F

I

I

T

G

G

P

I
x
T

G
|
G

C
|
C

S

-

R

-

L

M

P

Y

A

V

Y

A

L

N

S

T

S

S

Y

Y

G

G

-

C

-

G

-

P

-

W

-

R

-

V

-

P

-

W

F

I

H

H

G

A

V

Q

H
x
I

G

T

R

N

A

G

L

G

A

A

V

V

A

A

A

S

G

G

L

I

K

E

M

A

A

A

R

Y

P

K

D

A

L

M

-

I

-

R

-

K

-

T

-

D

-

A

-

E

-

F

-

P

T

N

V

I

L

I

V

V

A

M

G

A

G
|
G

D
|
D

G
|
G

D

G

G

A

Y

V

S

D

I

I

G

G

G

L

N

Q

H

A

F

L

L

S

H

A

A

M

C

L

R

Y

R

R

N

G

M

H

D

D

M

V

T

L

Y

F

L

I

V

C

M
x
Y

D

D

N
|
N

E

E

V
x
S

Y
|
Y

G
x
A

M
x
N

T
|
T

K

G

G

I

Q

Q

P

T

S

S

P

P

T

T

S

T

D

P

P

Y

D

G

W

A

H

N

S

T

K

T

L

F

A

T

P

P

H

P

G

G

-

E

-

V

-

P

T

E

G

G

I

K

T

K

A

L

F

F

-

P

-

K

-

D

N

N

P

P

L

K

G

V

V

I

A

A

L

H

A

G

-

H

A

P

G

E

A

L

S

K

F

Y

I

V

A

A

R

T

G

A

F

S

S

I

G

G

D

W

P

P

K

V

G

D

L

L

T

M

K

N

L

K

I

V

S

R

D

K

G

G

I

L

E

N

H

Q

K

E

G

G

F

P

S

A

V

Y

I

I

E

H

I

I

K

H

S

A

P

P

C
|
C

V

-

T

-

F

-

R

-

P

-

E

-

Q
x
P

K
|
K

A

G

W

W

Q

Q

active site: N143 (≠ M153)
binding magnesium ion: D110 (= D120), N138 (= N148), S140 (≠ V150)
binding iron/sulfur cluster: C24 (= C44), C27 (= C47), P29 (≠ H49), C52 (= C78), A142 (≠ G152), C225 (= C227), P226 (≠ Q234), K227 (= K235)
binding thiamine diphosphate: T50 (≠ I76), G51 (= G77), C52 (= C78), I74 (≠ H95), G109 (= G119), D110 (= D120), G111 (= G121), Y136 (≠ M146), N138 (= N148), S140 (≠ V150), Y141 (= Y151), A142 (≠ G152), N143 (≠ M153), T144 (= T154)

Q2RMD6 Pyruvate:ferredoxin oxidoreductase; PFOR; Pyruvate synthase; EC 1.2.7.1 from Moorella thermoacetica (strain ATCC 39073 / JCM 9320) (see paper)
28% identity, 39% coverage: 113:228/297 of query aligns to 960:1076/1171 of Q2RMD6

query
sites
Q2RMD6

T

S

V

I

L

W

V

I

A

I

G

G

D
|
D

G
|
G

D
x
W

G

A

Y

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

L

D

H

H

A

V

C

L

R

A

R

S

N

G

M

A

D

N

M

V

T

N

Y

V

L

L

V

V

M

L

D

D

N
x
T

E
|
E

V
|
V

Y
|
Y

G
x
S

M
x
N

T

T

K

G

G

G

Q

Q

P

S

S

S

P

K

T

A

S

T

D

Q

P

T

D

G

W

A

H

V

S

A

K

R

L

F

A

A

P

A

H

G

G

G

T

K

G

-

I

F

T

T

A

K

F

K

N

K

P

D

L

L

G

G

V

L

-

M

A

A

L

M

A

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

A

A

R

S

-

V

G

A

F

M

S

G

G

A

D

S

P

H

K

S

G

Q

L

L

T

M

K

K

L

A

I

L

S

I

D

E

G

A

I

E

E

K

H

Y

K

D

G

G

F

P

S

S

V

L

I

I

E

I

I

A

K

Y

S

A

P

P

C
|
C

V

I

D967 (= D120) binding Mg(2+)
DGW 967:969 (≠ DGD 120:122) binding thiamine diphosphate
T995 (≠ N148) binding Mg(2+)
TEVYSN 995:1000 (≠ NEVYGM 148:153) binding thiamine diphosphate
V997 (= V150) binding Mg(2+)
C1075 (= C227) binding [4Fe-4S] cluster

Sites not aligning to the query:

424:428 binding CoA
456 binding CoA
556 binding CoA
598 binding CoA
686 binding [4Fe-4S] cluster
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
696 binding [4Fe-4S] cluster
742 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
752 binding [4Fe-4S] cluster
809 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
814 binding thiamine diphosphate
837 binding [4Fe-4S] cluster; binding thiamine diphosphate

6ciqA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with coenzyme a bound (see paper)
28% identity, 39% coverage: 113:228/297 of query aligns to 959:1075/1169 of 6ciqA

query
sites
6ciqA

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

Y

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

L

D

H

H

A

V

C

L

R

A

R

S

N

G

M

A

D

N

M

V

T

N

Y

V

L

L

V

V

M

L

D

D

N
x
T

E

E

V
|
V

Y
|
Y

G
x
S

M
x
N

T
|
T

K

G

G

G

Q

Q

P

S

S

S

P

K

T

A

S

T

D

Q

P

T

D

G

W

A

H

V

S

A

K

R

L

F

A

A

P

A

H

G

G

G

T

K

G

-

I

F

T

T

A

K

F

K

N

K

P

D

L

L

G

G

V

L

-

M

A

A

L

M

A

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

A

A

R

S

-

V

G

A

F

M

S

G

G

A

D

S

P

H

K

S

G

Q

L

L

T

M

K

K

L

A

I

L

S

I

D

E

G

A

I

E

E

K

H

Y

K

D

G

G

F

P

S

S

V

L

I

I

E

I

I

A

K

Y

S

A

P

P

C
|
C

V
x
I

active site: N999 (≠ M153)
binding coenzyme a: N999 (≠ M153)
binding magnesium ion: D966 (= D120), T994 (≠ N148), V996 (= V150)
binding iron/sulfur cluster: S998 (≠ G152), C1074 (= C227), I1075 (≠ V228)
binding thiamine diphosphate: G965 (= G119), D966 (= D120), G967 (= G121), V996 (= V150), Y997 (= Y151), S998 (≠ G152), N999 (≠ M153), T1000 (= T154)

Sites not aligning to the query:

active site: 28, 61, 111
binding coenzyme a: 28, 423, 424, 427, 428, 553, 586
binding iron/sulfur cluster: 678, 680, 685, 686, 688, 689, 691, 695, 696, 700, 734, 741, 742, 743, 744, 745, 747, 751, 757, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 85, 813, 835, 836, 865

6cinB Crystal structure of pyruvate:ferredoxin oxidoreductase from moorella thermoacetica (see paper)
28% identity, 39% coverage: 113:228/297 of query aligns to 959:1075/1169 of 6cinB

query
sites
6cinB

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

Y

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

L

D

H

H

A

V

C

L

R

A

R

S

N

G

M

A

D

N

M

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T

N

Y

V

L

L

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V

M

L

D

D

N
x
T

E

E

V
|
V

Y
|
Y

G
x
S

M
x
N

T
|
T

K

G

G

G

Q

Q

P

S

S

S

P

K

T

A

S

T

D

Q

P

T

D

G

W

A

H

V

S

A

K

R

L

F

A

A

P

A

H

G

G

G

T

K

G

-

I

F

T

T

A

K

F

K

N

K

P

D

L

L

G

G

V

L

-

M

A

A

L

M

A

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

A

A

R

S

-

V

G

A

F

M

S

G

G

A

D

S

P

H

K

S

G

Q

L

L

T

M

K

K

L

A

I

L

S

I

D

E

G

A

I

E

E

K

H

Y

K

D

G

G

F

P

S

S

V

L

I

I

E

I

I

A

K

Y

S

A

P

P

C
|
C

V
x
I

active site: N999 (≠ M153)
binding magnesium ion: D966 (= D120), T994 (≠ N148), V996 (= V150)
binding iron/sulfur cluster: S998 (≠ G152), C1074 (= C227), I1075 (≠ V228)
binding thiamine diphosphate: G965 (= G119), D966 (= D120), G967 (= G121), V996 (= V150), Y997 (= Y151), S998 (≠ G152), N999 (≠ M153), T1000 (= T154)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 685, 686, 687, 688, 689, 691, 695, 700, 741, 742, 743, 744, 745, 746, 747, 751, 752, 757, 758, 807, 808, 811, 813, 836
binding thiamine diphosphate: 26, 27, 61, 834, 835, 836, 865

6cipA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with acetyl-tpp bound (see paper)
28% identity, 39% coverage: 113:228/297 of query aligns to 959:1075/1165 of 6cipA

query
sites
6cipA

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

Y

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

L

D

H

H

A

V

C

L

R

A

R

S

N

G

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A

D

N

M

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T

N

Y

V

L

L

V

V

M

L

D

D

N
x
T

E

E

V
|
V

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Y

G
x
S

M
x
N

T

T

K

G

G

G

Q

Q

P

S

S

S

P

K

T

A

S

T

D

Q

P

T

D

G

W

A

H

V

S

A

K

R

L

F

A

A

P

A

H

G

G

G

T

K

G

-

I

F

T

T

A

K

F

K

N

K

P

D

L

L

G

G

V

L

-

M

A

A

L

M

A

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

A

A

R

S

-

V

G

A

F

M

S

G

G

A

D

S

P

H

K

S

G

Q

L

L

T

M

K

K

L

A

I

L

S

I

D

E

G

A

I

E

E

K

H

Y

K

D

G

G

F

P

S

S

V

L

I

I

E

I

I

A

K

Y

S

A

P

P

C
|
C

V

I

active site: N999 (≠ M153)
binding 2-acetyl-thiamine diphosphate: G965 (= G119), D966 (= D120), G967 (= G121), V996 (= V150), S998 (≠ G152), N999 (≠ M153)
binding magnesium ion: D966 (= D120), T994 (≠ N148), V996 (= V150)
binding iron/sulfur cluster: S998 (≠ G152), C1074 (= C227)

Sites not aligning to the query:

active site: 28, 61, 111
binding 2-acetyl-thiamine diphosphate: 27, 61, 85, 111, 813, 834, 835, 836, 865
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836

6cioA Pyruvate:ferredoxin oxidoreductase from moorella thermoacetica with lactyl-tpp bound (see paper)
28% identity, 39% coverage: 113:228/297 of query aligns to 959:1075/1164 of 6cioA

query
sites
6cioA

T

S

V

I

L

W

V

I

A

I

G

G

G
|
G

D
|
D

G
|
G

D

W

G

A

Y

Y

S

D

I

I

G

G

G

Y

N

G

H

G

F

L

L

D

H

H

A

V

C

L

R

A

R

S

N

G

M

A

D

N

M

V

T

N

Y

V

L

L

V

V

M

L

D

D

N
x
T

E

E

V
|
V

Y
|
Y

G
x
S

M
x
N

T
|
T

K

G

G

G

Q

Q

P

S

S

S

P

K

T

A

S

T

D

Q

P

T

D

G

W

A

H

V

S

A

K

R

L

F

A

A

P

A

H

G

G

G

T

K

G

-

I

F

T

T

A

K

F

K

N

K

P

D

L

L

G

G

V

L

-

M

A

A

L

M

A

S

A

Y

G

G

A

Y

S

V

F

Y

I

V

A

A

R

S

-

V

G

A

F

M

S

G

G

A

D

S

P

H

K

S

G

Q

L

L

T

M

K

K

L

A

I

L

S

I

D

E

G

A

I

E

E

K

H

Y

K

D

G

G

F

P

S

S

V

L

I

I

E

I

I

A

K

Y

S

A

P

P

C
|
C

V
x
I

active site: N999 (≠ M153)
binding magnesium ion: D966 (= D120), T994 (≠ N148), V996 (= V150)
binding iron/sulfur cluster: C1074 (= C227), I1075 (≠ V228)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: G965 (= G119), D966 (= D120), G967 (= G121), V996 (= V150), Y997 (= Y151), S998 (≠ G152), N999 (≠ M153), T1000 (= T154)

Sites not aligning to the query:

active site: 28, 61, 111
binding iron/sulfur cluster: 455, 678, 680, 685, 686, 688, 689, 691, 695, 699, 700, 734, 741, 742, 744, 745, 747, 751, 757, 758, 807, 808, 811, 813, 836
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-carboxy-1-hydroxyethyl)-5-(2-{[hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: 26, 27, 28, 61, 85, 111, 813, 834, 835, 836, 865

Query Sequence

>WP_019896062.1 NCBI__GCF_000384235.1:WP_019896062.1
MNMTEATKTQMNKAQAVERLDQTPVEVRPAKPNDYLSQNHPVWCPGCGHFFVLRALTKAL
AWLNRPPEELAMITGIGCSSRLPAYLSSYGFHGVHGRALAVAAGLKMARPDLTVLVAGGD
GDGYSIGGNHFLHACRRNMDMTYLVMDNEVYGMTKGQPSPTSDPDWHSKLAPHGTGITAF
NPLGVALAAGASFIARGFSGDPKGLTKLISDGIEHKGFSVIEIKSPCVTFRPEQKAWQKQ
MRPLNIEPTPNKLEAAQAIHASDGFDTGIFYQADQPVYAPASEATHSLDEIEQELVL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory