SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020173599.1 NCBI__GCF_000385335.1:WP_020173599.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7rbxC Crystal structure of isocitrate lyase and phosphorylmutase:isocitrate lyase from brucella melitensis biovar abortus 2308 bound to itaconic acid (see paper)
73% identity, 96% coverage: 7:427/438 of query aligns to 3:424/425 of 7rbxC

query
sites
7rbxC

F

F

Q

Y

D

S

L

L

I

I

P

P

S

S

A

A

P

P

A

K

G

G

R

R

F

F

D

D

G

G

I

I

R

E

R

R

P

A

Y

H

G

T

P

A

E

E

E

D

V

V

K

K

R

R

L

L

R

R

G

G

S

S

F

V

P

E

I

I

A

K

Y

Y

T

S

L

L

A

A

E

E

R

M

S

G

A

A

N

N

K

R

L

L

W

W

K

K

L

L

M

I

Q

H

E

E

R

E

P

D

Y

F

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

S

G

G

N

N

Q

Q

A

A

M

M

Q

Q

M

M

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y

Y

L

L

S

S

G

G

W

W

Q

Q

V

V

A

A

D

D

A

A

N

N

V

T

A

A

G

S

A

A

M

M

Y

Y

P

P

D

D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

A

G

G

P

P

E

E

L

L

A

A

R

K

K

R

I

I

N

N

K

R

T

T

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

E

H

T

A

A

E

E

G

G

N

K

-

G

-

L

V

S

T

V

R

D

D

T

W

W

F

F

L

A

P

P

I

I

I

V

A

A

D

D

A

A

E

E

A

A

G

G

F

F

G

G

P

P

L

L

N

D

V

A

F

F

E

E

I

I

M

M

K

K

A

A

Y

Y

I

I

E

E

A

A

G

G

V

A

A

A

A

G

V

V

H

H

F

F

E

E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G

G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

A

Q

A

H

H

E

I

R

R

S

N

L

L

S

N

A

A

R

R

L

L

A

A

D

D

V

V

M

M

G

G

V

T

P

P

T

T

L

L

V

I

V

V

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

K

L

L

I

L

T

T

S

S

D

D

V

I

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

V

I

D

P

Y

D

E

S

A

-

G

R

R

T

T

P

A

E

E

G

G

F

F

Y

Y

R

Q

L

V

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

I

A

A

Y

Y

A

A

E

P

V

Y

S

C

D

D

M

L

L

I

W

W

W

M

E

E

T

T

S

S

H

K

P

P

D

D

L

L

G

A

E

Q

A

A

R

R

E

R

F

F

A

A

E

E

A

A

V

V

H

H

K

K

K

A

F

H

P

P

G

G

K

K

L

L

A

A

Y

Y

N

N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

N

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

R

E

E

L

L

G

G

A

A

M

M

G

G

Y

Y

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L

L

A

A

G

G

F

F

H

H

S

Q

L

L

N

N

L

Y

S

G

M

M

Y

F

E

E

L

L

A

A

L

R

G

G

Y

Y

K

K

D

D

R

R

G

Q

M

M

D

A

A

A

Y

Y

S

S

E

E

L

L

Q

Q

A

A

E

E

F

F

D

A

L

A

E

E

A

A

K

D

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

T

G

G

Y

Y

F

F

D

D

A

A

V

V

S

S

V

L

A

A

I

I

S

T

G

G

A

Q

S

S

T

T

A

A

M

M

G

K

E

E

S

S

T

T

E

E

S

T

A

A

Q

Q

F

F

K

K

binding 2-methylidenebutanedioic acid: C184 (= C186), H186 (= H188), R221 (= R223), S310 (= S313), S312 (= S315), T342 (= T345)

6lrtA Crystal structure of isocitrate lyase (caur_3889) from chloroflexus aurantiacus in complex with isocitrate and manganese ion
71% identity, 93% coverage: 18:426/438 of query aligns to 18:423/423 of 6lrtA

query
sites
6lrtA

R

R

F

F

D

A

G

G

I

I

R

V

R

R

P

P

Y

Y

G

T

P

P

E

E

E

D

V

V

K

Y

R

R

L

L

R

R

G

G

S

S

F

V

P

Q

I

I

A

E

Y

Y

T

T

L

L

A

A

E

R

R

M

S

G

A

A

N

E

K

R

L

L

W

W

K

N

L

L

M

L

Q

H

E

T

R

E

P

P

Y

Y

V

I

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

Q

A

A

M

M

Q

Q

M

Q

A

V

R

K

A

A

G

G

L

L

E

K

A

A

I

I

Y

Y

L

L

S

S

G

G

W

W

Q

Q

V

V

A

A

D

D

A

A

N

N

V

L

A

A

G

G

A

Q

M

M

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

G

P

P

E

Q

L

L

A

V

R

R

K

N

I

I

N

N

K

N

T

A

F

L

K

R

R

R

V

A

D

D

E

Q

I

I

E

Y

H

H

A

S

E

E

G

G

N

R

V

N

T

D

R

I

D

Y

W

W

F

F

L

A

P

P

I

I

I

V

A

A

D
|
D

A

A

E

E

A

A

G

G

F

F

G

G

P

P

L

L

N

N

V

V

F

F

E

E

I

I

M

M

K

K

A

A

Y

Y

I

I

E

E

A

A

G

G

V

A

A

A

A

G

V

V

H

H

F

F

E

E

D

D

Q

Q

L

L

A

A

S

S

E

E

K
|
K

K

K

C

C

G

G

H

H

L

M

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

A

H

A

E

I

R

R

S

N

L

L

S

V

A

A

R

R

L

L

A

A

D

D

V

V

M

M

G

G

V

V

P

P

T

T

L

I

V

I

V

V

A

A

R

R

T

T

D

D

A

A

E

N

S

A

A

A

K

T

L

L

I

L

T

T

S

S

D

D

V

I

D

D

E

E

R

R

D

D

R

R

P

P

F

F

I

C

I

T

P

G

D

E

S

-

R

R

T

T

P

S

E

E

G

G

F

F

Y

Y

R

R

L

V

K

R

D

-

G

-

T

A

G

G

L

L

A

D

H

Q

C

A

I

I

S

A

R

R

G

G

L

L

A

A

Y

Y

A

A

E

P

V

Y

S

A

D

D

M

M

L

I

W

W

W

C

E

E

T

T

S

S

H

E

P

P

D

N

L

L

G

E

E

E

A

A

R

R

E

R

F

F

A

A

E

E

A

A

V

I

H

H

K

A

K

Q

F

F

P

P

G

G

K

K

L

L

A

A

Y

Y

N

N

C

C

S

S

P

P

S

S

F

F

N

N

W

W

R

K

A

K

K

K

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

A

F

F

Q

Q

R

R

E

E

L

L

G

G

A

A

M

M

G

G

Y

Y

K

K

Y

F

Q

Q

F

F

V

V

T

T

L

L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L

Y

S

S

M

M

Y

F

E

E

L

L

A

A

L

R

G

N

Y

Y

K

R

D

D

R

R

G

G

M

M

D

A

A

A

Y

Y

S

S

E

E

L

L

Q

Q

A

A

E

E

F

F

D

A

L

A

E

E

A

A

K

Y

G

G

Y

Y

S

T

A

A

T

T

R

R

H

H

Q

Q

R

R

E

E

V

V

G

G

T

T

G

G

Y

Y

F

F

D

D

A

E

V

V

S

A

V

Q

A

V

I

I

S

A

G

G

A

E

S

I

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

E

Q

Q

F

F

binding manganese (ii) ion: D104 (= D104), D148 (= D148), K184 (= K184)

7cmyC Isocitrate lyase from bacillus cereus atcc 14579 in complex with magnessium ion, glyoxylate, and succinate
66% identity, 93% coverage: 18:426/438 of query aligns to 17:417/417 of 7cmyC

query
sites
7cmyC

R

R

F

W

D

E

G

G

I

I

R

T

R

R

P

P

Y

Y

G

S

P

A

E

E

E

D

V

V

K

I

R

R

L

L

R

R

G

G

S

S

F

I

P

D

I

I

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

K

L

L

W

W

K

T

L

S

M

L

Q

H

E

T

R

E

P

D

Y

Y

V

I

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

Q

A

A

M

M

Q

Q

M

Q

A

V

R

K

A

A

G

G

L

L

E

K

A

A

I

I

Y

Y

L

L

S

S

G

G

W

W

Q

Q

V

V

A

A

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

M

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

A

L

V

A

V

R

K

K

R

I

I

N

N

K

Q

T

T

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

Q

H

H

A

M

E

E

G

G

N

S

V

D

T

D

R

T

D

D

W

Y

F

F

L

V

P

P

I

I

I

V

A

A

D
|
D

A

A

E

E

A

A

G

G

F

F

G

G

P

Q

L

L

N

N

V

V

F

F

E

E

I

L

M

M

K

K

A

G

Y

M

I

I

E

E

A

A

G

G

V

A

A

S

A

G

V

V

H

H

F

F

E

E

D

D

Q

Q

L

L

A

S

S

S

E

E

K
|
K

K

K

C

C

G

G

H

H

L

L

G

G

K

K

V

V

L

L

P

P

T

T

A

Q

Q

T

H

A

E

V

R

R

S

N

L

L

S

I

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

M

G

G

V

V

P

P

T

T

L

I

V

I

V

V

A

A

R

R

T

T

D

D

A

A

E

D

S

A

A

A

K

D

L

L

I

I

T

T

S

S

D

D

V

I

D

D

E

P

R

V

D

D

R

K

P

A

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

P

E

E

G

G

F

F

Y

Y

R

R

L

T

K

N

D

-

G

-

T

A

G

G

L

L

A

D

H

Q

C

A

I

I

S

A

R

R

G

G

L

L

A

A

Y

Y

A

A

E

P

V

Y

S

A

D

D

M

L

L

V

W

W

W

C

E

E

T

T

S

S

H

E

P

P

D

N

L

L

G

E

E

D

A

A

R

K

E

R

F

F

A

A

E

D

A

A

V

I

H

H

K

K

K

E

F

H

P

P

G

G

K

K

L

L

A

A

Y

Y

N

N

C

C

S

S

P

P

S

S

F

F

N

N

W

W

R

K

A

Q

K

K

L

L

D

-

K

-

A

-

T

-

I

-

E

A

K

S

F

F

Q

Q

R

K

E

E

L

I

G

A

A

S

M

Y

G

G

Y

Y

K

K

Y

F

Q

Q

F

F

V

V

T

T

L

L

A

A

G

G

F

F

H

H

S

S

L

L

N

N

L

Y

S

G

M

M

Y

F

E

E

L

L

A

A

L

R

G

G

Y

Y

K

K

D

E

R

R

G

G

M

M

D

A

A

A

Y

Y

S

S

E

E

L

L

Q

Q

A

A

E

E

F

F

D

A

L

A

E

E

A

K

K

H

G

G

Y

Y

S

S

A

A

T

T

R

R

H

H

Q

Q

R

R

E

E

V

V

G

G

T

T

G

G

Y

Y

F

F

D

D

A

E

V

V

S

A

V

Q

A

V

I

I

S

T

G

G

A

T

S

S

T

T

A

A

M

L

G

K

E

G

S

S

T

T

E

E

S

E

A

A

Q

Q

F

F

binding magnesium ion: D103 (= D104), D147 (= D148), K183 (= K184)

7rb1A Isocitrate lyase-1 from mycobacterium tuberculosis covalently modified by 5-descarboxy-5-nitro-d-isocitric acid (see paper)
59% identity, 93% coverage: 18:426/438 of query aligns to 22:427/427 of 7rb1A

query
sites
7rb1A

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y
|
Y

L

L

S
|
S

G

G

W
|
W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D

D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E

E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H

H

F

W

E

E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G
|
G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A
|
A

E

P

V

F

S
x
A

D

D

M

L

L

I

W

W

W

M

E

E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K
x
Q

L

M

L

L

A

A

Y

Y

N

N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L

L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L

Y

S

S

M

M

Y

F

E

D

L

L

A

A

L

Y

G

G

Y

Y

K

A

D

Q

R

N

G

Q

M

M

D

S

A

A

Y

Y

S

V

E

E

L

L

Q

Q

Q

E

A

R

E

E

F

F

D

A

L

A

E

E

A

E

K

R

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

A

G

G

Y

Y

F

F

D

D

A

R

V

I

S

A

V

T

A

T

I

V

S

D

G

P

G

N

A

-

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

G

Q

Q

F

F

binding dihydroxyacetic acid: Y89 (= Y85), S91 (= S87), W93 (= W89), D153 (= D148), R228 (= R223), T347 (= T345)
binding (3E)-3-(hydroxyimino)propanoic acid: C191 (= C186), G192 (= G187), H193 (= H188), R228 (= R223), S315 (= S313), S317 (= S315), T347 (= T345)
binding magnesium ion: A276 (= A274), A279 (≠ S277), Q308 (≠ K306)

6wsiA Intact cis-2,3-epoxysuccinic acid bound to isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
59% identity, 93% coverage: 18:426/438 of query aligns to 22:427/427 of 6wsiA

query
sites
6wsiA

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y
|
Y

L

L

S

S

G

G

W

W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E
|
E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H
|
H

F

W

E
|
E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G
|
G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A
|
A

E

P

V

F

S
x
A

D

D

M

L

L

I

W

W

W

M

E
|
E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K
x
Q

L

M

L

L

A

A

Y

Y

N
|
N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L

L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L

Y

S

S

M

M

Y

F

E

D

L

L

A

A

L

Y

G

G

Y

Y

K

A

D

Q

R

N

G

Q

M

M

D

S

A

A

Y

Y

S

V

E

E

L

L

Q

Q

Q

E

A

R

E

E

F

F

D

A

L

A

E

E

A

E

K

R

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

A

G

G

Y

Y

F

F

D

D

A

R

V

I

S

A

V

T

A

T

I

V

S

D

G

P

G

N

A

-

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

G

Q

Q

F

F

active site: Y89 (= Y85), D108 (= D104), D153 (= D148), E155 (= E150), H180 (= H175), E182 (= E177), C191 (= C186), H193 (= H188), R228 (= R223), E285 (= E283), Q308 (≠ K306), S315 (= S313), S317 (= S315)
binding magnesium ion: A276 (= A274), A279 (≠ S277), Q308 (≠ K306)
binding (2R,3S)-oxirane-2,3-dicarboxylic acid: C191 (= C186), G192 (= G187), H193 (= H188), R228 (= R223), E285 (= E283), N313 (= N311), S315 (= S313), S317 (= S315), T347 (= T345)

6vb9A Covalent adduct of cis-2,3-epoxysuccinic acid with isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
59% identity, 93% coverage: 18:426/438 of query aligns to 22:427/427 of 6vb9A

query
sites
6vb9A

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y
|
Y

L

L

S
|
S

G
|
G

W
|
W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E
|
E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H
|
H

F

W

E
|
E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G

G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A
|
A

E

P

V

F

S
x
A

D

D

M

L

L

I

W
|
W

W

M

E
|
E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K
x
Q

L

M

L

L

A

A

Y

Y

N

N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L

L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L

Y

S

S

M

M

Y

F

E

D

L

L

A

A

L

Y

G

G

Y

Y

K

A

D

Q

R

N

G

Q

M

M

D

S

A

A

Y

Y

S

V

E

E

L

L

Q

Q

Q

E

A

R

E

E

F

F

D

A

L

A

E

E

A

E

K

R

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

A

G

G

Y

Y

F

F

D

D

A

R

V

I

S

A

V

T

A

T

I

V

S

D

G

P

G

N

A

-

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

G

Q

Q

F

F

active site: Y89 (= Y85), D108 (= D104), D153 (= D148), E155 (= E150), H180 (= H175), E182 (= E177), C191 (= C186), H193 (= H188), R228 (= R223), E285 (= E283), Q308 (≠ K306), S315 (= S313), S317 (= S315)
binding magnesium ion: A276 (= A274), A279 (≠ S277), Q308 (≠ K306)
binding oxalic acid: Y89 (= Y85), S91 (= S87), G92 (= G88), W93 (= W89), D153 (= D148), C191 (= C186), R228 (= R223), W283 (= W281), T347 (= T345)

5dqlA Crystal structure of 2-vinyl glyoxylate modified isocitrate lyase from mycobacterium tuberculosis (see paper)
59% identity, 93% coverage: 18:426/438 of query aligns to 22:427/427 of 5dqlA

query
sites
5dqlA

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y
|
Y

L

L

S

S

G

G

W
|
W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E
|
E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H
|
H

F

W

E
|
E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G

G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A
|
A

E

P

V

F

S
x
A

D

D

M

L

L

I

W

W

W

M

E
|
E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K
x
Q

L

M

L

L

A

A

Y

Y

N

N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L
|
L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L

Y

S

S

M

M

Y

F

E

D

L

L

A

A

L

Y

G

G

Y

Y

K

A

D

Q

R

N

G

Q

M

M

D

S

A

A

Y

Y

S

V

E

E

L

L

Q

Q

Q

E

A

R

E

E

F

F

D

A

L

A

E

E

A

E

K

R

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

A

G

G

Y

Y

F

F

D

D

A

R

V

I

S

A

V

T

A

T

I

V

S

D

G

P

G

N

A

-

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

G

Q

Q

F

F

active site: Y89 (= Y85), D108 (= D104), D153 (= D148), E155 (= E150), H180 (= H175), E182 (= E177), C191 (= C186), H193 (= H188), R228 (= R223), E285 (= E283), Q308 (≠ K306), S315 (= S313), S317 (= S315)
binding magnesium ion: A276 (= A274), A279 (≠ S277), Q308 (≠ K306)
binding 4-hydroxy-2-oxobutanoic acid: W93 (= W89), D108 (= D104), C191 (= C186), H193 (= H188), S315 (= S313), S317 (= S315), T347 (= T345), L348 (= L346)

6xppA Crystal structure of itaconate modified mycobaterium tuberculosis isocitrate lyase (see paper)
59% identity, 93% coverage: 18:426/438 of query aligns to 21:426/426 of 6xppA

query
sites
6xppA

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y
|
Y

L

L

S

S

G

G

W
|
W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E
|
E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H
|
H

F

W

E
|
E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G

G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A
|
A

E

P

V

F

S
x
A

D

D

M

L

L

I

W

W

W

M

E
|
E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K
x
Q

L

M

L

L

A

A

Y

Y

N
|
N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L

L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L

Y

S

S

M

M

Y

F

E

D

L

L

A

A

L

Y

G

G

Y

Y

K

A

D

Q

R

N

G

Q

M

M

D

S

A

A

Y

Y

S

V

E

E

L

L

Q

Q

Q

E

A

R

E

E

F

F

D

A

L

A

E

E

A

E

K

R

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

A

G

G

Y

Y

F

F

D

D

A

R

V

I

S

A

V

T

A

T

I

V

S

D

G

P

G

N

A

-

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

G

Q

Q

F

F

active site: Y88 (= Y85), D107 (= D104), D152 (= D148), E154 (= E150), H179 (= H175), E181 (= E177), C190 (= C186), H192 (= H188), R227 (= R223), E284 (= E283), Q307 (≠ K306), S314 (= S313), S316 (= S315)
binding 2-methylidenebutanedioic acid: W92 (= W89), C190 (= C186), H192 (= H188), R227 (= R223), N312 (= N311), S314 (= S313), S316 (= S315), T346 (= T345)
binding magnesium ion: A275 (= A274), A278 (≠ S277), Q307 (≠ K306)

6c4aA Crystal structure of 3-nitropropionate modified isocitrate lyase from mycobacterium tuberculosis with pyruvate (see paper)
59% identity, 93% coverage: 18:426/438 of query aligns to 23:428/428 of 6c4aA

query
sites
6c4aA

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y
|
Y

L

L

S
|
S

G
|
G

W
|
W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E
|
E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H
|
H

F

W

E
|
E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G

G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A
|
A

E

P

V

F

S
x
A

D

D

M

L

L

I

W
|
W

W

M

E
|
E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K
x
Q

L

M

L

L

A

A

Y

Y

N

N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L

L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L
x
Y

S
|
S

M

M

Y

F

E

D

L

L

A

A

L

Y

G

G

Y

Y

K

A

D

Q

R

N

G

Q

M

M

D

S

A

A

Y

Y

S

V

E

E

L

L

Q

Q

Q

E

A
x
R

E

E

F

F

D

A

L

A

E

E

A

E

K

R

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

A

G

G

Y

Y

F

F

D

D

A

R

V

I

S

A

V

T

A

T

I

V

S

D

G

P

G

N

A

-

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

G

Q

Q

F

F

active site: Y90 (= Y85), D109 (= D104), D154 (= D148), E156 (= E150), H181 (= H175), E183 (= E177), C192 (= C186), H194 (= H188), R229 (= R223), E286 (= E283), Q309 (≠ K306), S316 (= S313), S318 (= S315)
binding 3-nitropropanoic acid: Y357 (≠ L354), S358 (= S355), R380 (≠ A377)
binding magnesium ion: A277 (= A274), A280 (≠ S277), Q309 (≠ K306)
binding pyruvic acid: Y90 (= Y85), S92 (= S87), G93 (= G88), W94 (= W89), D154 (= D148), C192 (= C186), R229 (= R223), W284 (= W281), T348 (= T345)

P9WKK7 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
59% identity, 93% coverage: 18:426/438 of query aligns to 22:427/428 of P9WKK7

query
sites
P9WKK7

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y

Y

L

L

S
|
S

G
|
G

W
|
W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D

D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E

E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H

H

F

W

E

E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
|
C

G
|
G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A

A

E

P

V

F

S

A

D

D

M

L

L

I

W

W

W

M

E

E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K

Q

L

M

L

L

A

A

Y

Y

N
|
N

C
|
C

S
|
S

P
|
P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R
x
K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I

T
|
T

L

L

A

A

G

G

F

F

H

H

S

A

L

L

N

N

L

Y

S

S

M

M

Y

F

E

D

L

L

A

A

L

Y

G

G

Y

Y

K

A

D

Q

R

N

G

Q

M

M

D

S

A

A

Y

Y

S

V

E

E

L

L

Q

Q

Q

E

A

R

E

E

F

F

D

A

L

A

E

E

A

E

K

R

G

G

Y

Y

S

T

A

A

T

T

R

K

H

H

Q

Q

R

R

E

E

V

V

G

G

T

A

G

G

Y

Y

F

F

D

D

A

R

V

I

S

A

V

T

A

T

I

V

S

D

G

P

G

N

A

-

S

S

T

T

A

A

M

L

G

T

E

G

S

S

T

T

E

E

S

E

A

G

Q

Q

F

F

SGW 91:93 (= SGW 87:89) binding substrate
D153 (= D148) binding Mg(2+)
C191 (= C186) mutation to S: Adopts a conformation almost identical to the wild-type.
GH 192:193 (= GH 187:188) binding substrate
R228 (= R223) binding substrate
NCSPS 313:317 (= NCSPS 311:315) binding substrate
K334 (≠ R332) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
T347 (= T345) binding substrate

1f8iA Crystal structure of isocitrate lyase:nitropropionate:glyoxylate complex from mycobacterium tuberculosis (see paper)
59% identity, 93% coverage: 18:426/438 of query aligns to 22:427/427 of 1f8iA

query
sites
1f8iA

R

R

F

W

D

K

G

D

I

V

R

T

R

R

P

T

Y

Y

G

S

P

A

E

E

E

D

V

V

K

V

R

A

L

L

R

Q

G

G

S

S

F

V

P

V

I

E

A

E

Y

H

T

T

L

L

A

A

E

R

R

R

S

G

A

A

N

E

K

V

L

L

W

W

K

E

L

Q

M

L

Q

H

E

D

R

L

P

E

Y

W

V

V

H

N

S

A

L

L

G

G

A

A

V

L

T

T

G

G

N

N

Q

M

A

A

M

V

Q

Q

M

Q

A

V

R

R

A

A

G

G

L

L

E

K

A

A

I

I

Y
|
Y

L

L

S
|
S

G

G

W
|
W

Q

Q

V

V

A

A

A

G

D

D

A

A

N

N

V

L

A

S

G

G

A

H

M

T

Y

Y

P

P

D
|
D

Q

Q

S

S

L

L

Y

Y

P

P

A

A

N

N

S

S

G

V

P

P

E

Q

L

V

A

V

R

R

K

R

I

I

N

N

K

N

T

A

F

L

K

Q

R

R

V

A

D

D

E

Q

I

I

E

A

H

K

A

I

E

E

G

G

N

D

V

T

T

S

-

V

R

E

D

N

W

W

F

L

L

A

P

P

I

I

I

V

A

A

D
|
D

A

G

E
|
E

A

A

G

G

F

F

G

G

P

A

L

L

N

N

V

V

F

Y

E

E

I

L

M

Q

K

K

A

A

Y

L

I

I

E

A

A

A

G

G

V

V

A

A

A

G

V

S

H
|
H

F

W

E
|
E

D

D

Q

Q

L

L

A

A

S

S

E

E

K

K

C
x
S

G

G

H
|
H

L

L

G

G

K

K

V

V

L

L

L

I

P

P

T

T

A

Q

Q

Q

H

H

E

I

R

R

S

T

L

L

S

T

A

S

A

A

R

R

L

L

A

A

D

D

V

V

M

A

G

D

V

V

P

P

T

T

L

V

V

V

V

I

A

A

R
|
R

T

T

D

D

A

A

E

E

S

A

A

A

K

T

L

L

I

I

T

T

S

S

D

D

V

V

D

D

E

E

R

R

D

D

R

Q

P

P

F

F

I

I

I

T

P

G

D

E

S

-

R

R

T

T

P

R

E

E

G

G

F

F

Y

Y

R

R

L

T

K

K

D

N

G

G

T

-

G

-

L

I

A

E

H

P

C

C

I

I

S

A

R

R

G

A

L

K

A

A

Y

Y

A

A

E

P

V

F

S

A

D

D

M

L

L

I

W

W

W

M

E
|
E

T

T

S

G

H

T

P

P

D

D

L

L

G

E

E

A

A

A

R

R

E

Q

F

F

A

S

E

E

A

A

V

V

H

K

K

A

K

E

F

Y

P

P

G

D

K
x
Q

L

M

L

L

A

A

Y

Y

N

N

C

C

S
|
S

P

P

S
|
S

F

F

N

N

W

W

R

K

A

K

K

H

L

L

D

D

K

D

A

A

T

T

I

I

E

A

K

K

F

F

Q

Q

R

K

E

E

L

L

G

A

A

A

M

M

G

G

Y

F

K

K

Y

F

Q

Q

F

F

V

I