SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020174170.1 NCBI__GCF_000385335.1:WP_020174170.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4yerA Crystal structure of an abc transporter atp-binding protein (tm_1403) from thermotoga maritima msb8 at 2.35 a resolution
35% identity, 83% coverage: 26:248/269 of query aligns to 5:228/285 of 4yerA

query
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4yerA

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binding adenosine-5'-diphosphate: F14 (≠ Y35), F17 (≠ R38), N39 (= N60), G40 (= G61), G42 (= G63), K43 (= K64), T44 (≠ S65), T45 (= T66), T135 (≠ D156), F136 (≠ L157), S137 (= S158)

P34358 ABC transporter ced-7; Cell death protein 7 from Caenorhabditis elegans (see 2 papers)
32% identity, 87% coverage: 6:238/269 of query aligns to 509:759/1704 of P34358

query
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P34358

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K586 (= K64) mutation to R: Cell corpses not engulfed.
E639 (≠ Q117) mutation to G: Cell corpses not engulfed.

Sites not aligning to the query:

126 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1012 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1045 modified: carbohydrate, N-linked (GlcNAc...) asparagine
1417 K→R: Some cell corpses not engulfed.

8tzjA Cryo-em structure of vibrio cholerae ftse/ftsx complex (see paper)
35% identity, 77% coverage: 26:232/269 of query aligns to 3:215/220 of 8tzjA

query
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8tzjA

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binding adenosine-5'-diphosphate: Y12 (= Y35), R16 (= R38), A18 (= A40), G39 (= G61), G41 (= G63), K42 (= K64), S43 (= S65)

Q5M244 Energy-coupling factor transporter ATP-binding protein EcfA2; ECF transporter A component EcfA2; EC 3.6.3.- from Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) (see paper)
31% identity, 82% coverage: 26:246/269 of query aligns to 3:235/280 of Q5M244

query
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Q5M244

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Q97 (≠ L109) mutation to A: No effect on ATPase, 10-fold decrease in riboflavin uptake; when associated with A-90 in EcfA1.
E171 (= E182) mutation to Q: 10-fold decrease in ATPase and riboflavin uptake; when associated with Q-163 in EcfA1.

Q9NP58 ATP-binding cassette sub-family B member 6; ABC-type heme transporter ABCB6; Mitochondrial ABC transporter 3; Mt-ABC transporter 3; P-glycoprotein-related protein; Ubiquitously-expressed mammalian ABC half transporter; EC 7.6.2.5 from Homo sapiens (Human) (see 11 papers)
34% identity, 87% coverage: 19:252/269 of query aligns to 578:821/842 of Q9NP58

query
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Q9NP58

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M

G579 (≠ A20) to E: in DUH3; the protein is retained in the Golgi apparatus. Does not affect subcellular location in early melanosome and lysosome. Does not rescue the normal amyloid fibril formation and normal maturation of pigmented melanosomes. Does not influence trafficking of melanosomal proteins.; dbSNP:rs397514758
G588 (≠ A24) to S: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs145526996
Y599 (= Y35) binding ATP
623:634 (vs. 58:69, 58% identical) binding ATP
K629 (= K64) mutation to A: Abolishes ATP hydrolysis. Abolishes coproporphyrin III transport.; mutation to M: Does not affect subcellular location in early melanosome and lysosome. Does not rescue the normal amyloid fibril formation and normal maturation of pigmented melanosomes. Does not influence trafficking of melanosomal proteins. Fails to rescue vacuolar sequestration of cadmium in Schizosaccharomyces pombe and Caenorhabditis elegans strains defective for HMT-1. Fails to rescue the cadmium tolerance in Schizosaccharomyces pombe and Caenorhabditis elegans strains defective for HMT-1. Does not rescue vacuolar cadmium levels in hmt-1 mutant S.pombe.
N677 (≠ M111) mutation to Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-447; Q-498; and Q-775. Does not affect trafficking from endoplasmic reticulum; when associated with Q-447; Q-498; and Q-775.
A681 (≠ Q116) to T: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs142421126
N775 (≠ H205) mutation to Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-447; Q-498 and Q-677. Does not affect trafficking from endoplasmic reticulum; when associated with Q-447; Q-498 and Q-677.
L811 (≠ V243) to V: in MCOPCB7; hypomorphic mutation; dbSNP:rs387906910

Sites not aligning to the query:

1:205 Required for the lysosomal targeting
1:236 Required for ATPase activity
6 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Loss of N-glycosylation; when associated with Q-447; Q-498; Q-677 and Q-775. Does not affect substrate binding.
8 modified: Disulfide link with 26; C→G: Loss of N-glycosylation.; C→S: Does not affect substrate binding. Does not affect N-glycosylation. Impairs endoplasmic reticulum exit. Impairs endoplasmic reticulum exit; when associated with C-8. Increases ABCB6 proteasomal degradation. Affects protein stability. Does not affect migration in the presence of DTT; when associated with A-50 and A-120.
26 modified: Disulfide link with 8; C→A: Decreases protein expression. Affects protein stability. Loss of ability to stimulate porphyrin synthesis.; C→S: Decreases protein expression. Impairs endoplasmic reticulum exit; when associated with C-8. Affects protein stability.
50 C→A: Increases migration in the absence of DTT; when associated with A-120. Reduces migration in with the presence of DTT; when associated with A-120.
57 A → T: in MCOPCB7; uncertain significance; hypomorphic mutation; dbSNP:rs387906911
69 R → G: in a breast cancer sample; somatic mutation
120 C→A: Increases migration in the absence of DTT; when associated with A-50. Reduces migration in with the presence of DTT; when associated with A-50.
170 S → G: in DUH3; the protein is retained in the Golgi apparatus; dbSNP:rs397514757
192 R → Q: decrease expression; does not affect substrate binding; does not affect ATP-binding; loss of plasma membrane expression; dbSNP:rs150221689
276 R → W: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs57467915
286 Y→A: Loss of substrate-stimulate ATPase activity. Impairs protein expression.
356 L → P: in DUH3; the protein is retained in the Golgi apparatus; dbSNP:rs397514756
447 N→Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-498; Q-677 and Q-775. Does not affect trafficking from endoplasmic reticulum; when associated with Q-498; Q-677 and Q-775.
492 A → T: may be a modifier of disease severity in porphyria patients; increases expression; does not affect substrate binding; impairs ATP-binding; Loss of ATP-dependent coproporphyrin III transport; Highly decrease plasma membrane expression; dbSNP:rs147445258
498 N→Q: Does not affect N-glycosylation. Does not affect N-glycosylation; when associated with Q-447; Q-677 and Q-775. Does not affect trafficking from endoplasmic reticulum; when associated with Q-447; Q-677 and Q-775.
521 T → S: may be a modifier of disease severity in porphyria patients; loss of expression; dbSNP:rs149363094
531 V→A: Loss of substrate-stimulate ATPase activity. Impairs protein expression.
542 M→A: Loss of substrate-stimulate ATPase activity.
546 W→A: Loss of substrate-stimulate ATPase activity. Impairs protein expression.; W→F: Does not affect substrate-stimulate ATPase activity.; W→V: Loss of substrate-stimulate ATPase activity. Impairs protein expression.

3nhaA Nucleotide binding domain of human abcb6 (adp mg bound structure) (see paper)
34% identity, 87% coverage: 19:252/269 of query aligns to 29:271/278 of 3nhaA

query
sites
3nhaA

P

P

A

G

E

A

G

G

P

P

-

L

-

R

-

F

-

Q

-

K

A

G

A

R

L

I

S

E

V

F

E

E

G

N

V

V

S

H

H

F

S

S

Y
|
Y

G

A

K

D

R

R

K

E

A
x
T

L

L

D

Q

N

D

V

V

S

S

F

F

S

T

V

V

K

M

P

P

S

G

S

Q

F

T

T

L

V

A

L

L

L

V

G

G

L

P

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

F

L

S

R

L

L

I

L

T

F

R

R

L

F

Y

Y

S

D

T

I

Q

S

T

S

G

G

K

C

I

I

K

R

I

I

F

D

G

G

Y

Q

E

D

V

I

G

S

Q

Q

D

V

P

T

G

Q

E

A

A

S

L

L

R

R

-

S

R

H

L

I

G

G

V

V

F

P

Q

Q

A

D

R

T

T

V

L

L

D

F

M

N

D

D

L

-

S

T

I

I

Q

A

Q

D

N

N

L

I

L

R

Y

Y

H

G

A

R

A

V

L

T

H

A

G

G

I

N

G

D

A

E

A

V

E

E

T

A

K

A

A

A

R

Q

A

A

A

G

V

I

L

H

E

D

Q

A

I

I

-

M

A

A

M

F

T

P

D

E

R

G

T

Y

K

R

D

T

K

Q

V

V

R

G

D

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

Q

E

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

T

L

I

L

L

H

K

H

A

P

P

Q

G

M

I

L

I

L

L

D

D

E

E

P

A

T

T

V

S

G

A

L

L

D

D

I

T

K

S

A

N

R

E

A

R

D

A

I

I

L

Q

K

A

H

S

V

L

R

A

D

K

L

V

V

C

A

A

H

N

S

R

G

T

I

T

G

I

V

V

L

V

W

-

T

-

T

A

H

H

L

R

I

L

D

S

E

T

V

V

N

V

P

N

D

A

D

D

D

Q

V

I

V

L

V

V

L

I

H

K

Q

D

G

G

K

C

I

I

L

V

D

E

K

R

G

G

R

R

V

H

S

E

D

A

V

L

A

S

R

R

A

G

G

G

V

V

-

Y

A

A

D

D

M

M

binding adenosine-5'-diphosphate: Y50 (= Y35), T55 (≠ A40), G76 (= G61), G78 (= G63), K79 (= K64), S80 (= S65), T81 (= T66)

1g6hA Crystal structure of the adp conformation of mj1267, an atp-binding cassette of an abc transporter (see paper)
33% identity, 80% coverage: 26:239/269 of query aligns to 5:233/254 of 1g6hA

query
sites
1g6hA

L

L

S

R

V

T

E

E

G

N

V

I

S

V

H

K

S

Y

Y
x
F

G

G

K

E

R
x
F

K

K

A

A

L

L

D

D

N

G

V

V

S

S

F

I

S

S

V

V

K

N

P

K

S

G

S

D

F

V

T

T

V

L

L

I

G

G

L

P

N
|
N

G
|
G

A

S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

I

S

N

L

V

I

I

T

T

R

G

L

F

Y

L

S

K

T

A

Q

D

T

E

G

G

K

R

I

V

K

Y

I

F

F

E

G

N

Y

K

E

D

V

I

-

T

G

N

Q

K

D

E

P

P

G

A

E

E

A

-

L

L

R

Y

R

H

L

Y

G

G

V

I

V

V

-

R

-

T

F

F

Q

Q

A

T

R

P

T

Q

L

P

D

L

M

K

D

E

L

M

S

T

I

V

Q

L

Q

E

N

N

L

L

Y

I

H

G

A

E

A

I

L

C

H

P

G

G

I

E

G

S

-

P

-

L

-

N

-

S

-

L

-

F

-

Y

-

K

-

W

-

I

-

P

-

K

A

E

E

E

T

M

K

V

A

E

R

K

A

A

A

F

A

K

V

I

L

L

E

E

Q

F

I

L

A

K

M

L

T

S

D

H

R

L

T

Y

K

D

D

R

K

K

V

A

R

G

D

E

L

L

S

S

G

G

Q

Q

M

M

R

K

R

L

V

V

E

E

I

I

A

G

R

R

A

A

L

L

L

M

H

T

H

N

P

P

Q

K

M

M

L

I

L

V

L

M

D

D

E

E

P

P

T

I

V

A

G

G

L

V

D

A

I

P

K

G

A

L

R

A

A

H

D

D

I

I

L

F

K

N

H

H

V

V

R

L

D

E

L

L

V

K

A

A

H

-

S

K

G

G

I

I

G

T

V

F

L

L

W

I

T

I

T

E

H

H

L

R

I

L

D

D

E

I

V

V

-

L

N

N

P

Y

D

I

D

D

D

H

V

L

V

Y

V

V

L

M

H

F

Q

N

G

G

K

Q

I

I

L

I

D

A

K

E

G

G

R

R

binding adenosine-5'-diphosphate: F14 (≠ Y35), F17 (≠ R38), N39 (= N60), G40 (= G61), G42 (= G63), K43 (= K64), S44 (= S65), T45 (= T66)

1g9xB Characterization of the twinning structure of mj1267, an atp-binding cassette of an abc transporter (see paper)
33% identity, 80% coverage: 26:239/269 of query aligns to 5:233/253 of 1g9xB

query
sites
1g9xB

L

L

S

R

V

T

E

E

G

N

V

I

S

V

H

K

S

Y

Y
x
F

G

G

K

E

R
x
F

K

K

A

A

L

L

D

D

N

G

V

V

S

S

F

I

S

S

V

V

K

C

P

K

S

G

S

D

F

V

T

T

V

L

L

I

G

G

L

P

N

N

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

I

S

N

L

V

I

I

T

T

R

G

L

F

Y

L

S

K

T

A

Q

D

T

E

G

G

K

R

I

V

K

Y

I

F

F

E

G

N

Y

K

E

D

V

I

-

T

G

N

Q

K

D

E

P

P

G

A

E

E

A

-

L

L

R

Y

R

H

L

Y

G

G

V

I

V

V

-

R

-

T

F

F

Q

Q

A

T

R

P

T

Q

L

P

D

L

M

K

D

E

L

M

S

T

I

V

Q

L

Q

E

N

N

L

L

Y

I

-

G

-

E

-

I

-

N

-

P

-

G

H

E

A

S

A

P

L

L

H

N

G

S

I

L

-

F

-

Y

-

K

-

W

-

I

-

P

G

K

A

E

E

E

T

M

K

V

A

E

R

K

A

A

A

F

A

K

V

I

L

L

E

E

Q

F

I

L

A

K

M

L

T

S

D

H

R

L

T

Y

K

D

D

R

K

K

V

A

R

G

D

E

L

L

S

S

G

G

Q

Q

M

M

R

K

R

L

V

V

E

E

I

I

A

G

R

R

A

A

L

L

L

M

H

T

H

N

P

P

Q

K

M

M

L

I

L

V

L

M

D

D

E
|
E

P

P

T

I

V

A

G

G

L

V

D

A

I

P

K

G

A

L

R

A

A

H

D

D

I

I

L

F

K

N

H

H

V

V

R

L

D

E

L

L

V

K

A

A

H

-

S

K

G

G

I

I

G

T

V

F

L

L

W

I

T

I

T

E

H

H

L

R

I

L

D

D

E

I

V

V

-

L

N

N

P

Y

D

I

D

D

D

H

V

L

V

Y

V

V

L

M

H

F

Q

N

G

G

K

Q

I

I

L

I

D

A

K

E

G

G

R

R

binding adenosine-5'-diphosphate: F14 (≠ Y35), F17 (≠ R38), G40 (= G61), S41 (≠ A62), G42 (= G63), K43 (= K64), S44 (= S65), T45 (= T66)
binding magnesium ion: S44 (= S65), E176 (= E182)

Q99758 Phospholipid-transporting ATPase ABCA3; ABC-C transporter; ATP-binding cassette sub-family A member 3; ATP-binding cassette transporter 3; ATP-binding cassette 3; Xenobiotic-transporting ATPase ABCA3; EC 7.6.2.1; EC 7.6.2.2 from Homo sapiens (Human) (see 15 papers)
31% identity, 75% coverage: 38:238/269 of query aligns to 546:745/1704 of Q99758

query
sites
Q99758

R

R

K

A

A

A

L

V

D

R

N

D

V

L

S

N

F

L

S

N

V

L

K

Y

P

E

S

G

S

Q

F

I

T

T

V

V

L

L

G

G

L

H

N
|
N

G

G

A

A

G

G

K

K

S

T

T

T

L

T

F

L

S

S

L

M

I
x
L

T

T

R

G

L

L

Y

F

S

P

T

P

Q

T

T

S

G

G

K

R

I

A

K

Y

I

I

F

S

G

G

Y

Y

E

E

V

I

G

S

Q

Q

D

D

P

M

G

V

E

Q

A

I

L
x
R

R

K

R

S

L

L

G

G

V

L

V

C

F

P

Q

Q

A

H

R

D

T

I

L

L

D

F

M

D

D

N

L

L

S

T

I

V

Q

A

Q

E

N

H

L

L

L

Y

Y

F

H

Y

A

A

A

Q

L

L

H

K

G

G

I

L

G

S

A

R

A

Q

E

K

T

C

K

P

A

E

R

E

A

V

A

K

A

Q

V

M

L

L

E

H

Q

I

I

I

A

G

M

L

T

E

D

D

R

K

T

W

K

N

D

S

K

R

V

S

R

R

D

F

L

L

S

S

G

G

Q

M

M

R

R

R

R

K

V

L

E

S

I

I

A

G

R

I

A

A

L

L

L

I

H

A

H

G

P

S

Q

K

M

V

L

L

L

I

L

L

D

D

E

E

P

P

T

T

V
x
S

G

G

L

M

D

D

I

A

K

I

A

S

R

R

A

-

D

-

I

-

L

-

K

R

H

A

V

I

R

W

D

D

L

L

V

L

A

Q

H

R

-

Q

-

K

S

S

G

D

I

R

G

T

V

I

L

V

W

L

T

T

H

H

L

F

I

M

D

D

E

E

V

A

N

D

-

L

P

L

D

G

D

D

D

R

V

I

V

A

V

I

L

M

H

A

Q

K

G

G

K

E

I

L

L

Q

D

C

K

C

G

G

N568 (= N60) to D: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; does not affect protein expression; does not affect multivesicular bodies and lamellar bodies location; affects multivesicular bodies and lamellar bodies development; loss of phosphatidylcholine transport; does not affect cholesterol transport; dbSNP:rs121909184
L579 (≠ I71) to P: in SMDP3; uncertain significance
R605 (≠ L97) to Q: in SMDP3; uncertain significance; dbSNP:rs760006956
S693 (≠ V185) mutation to L: Does not affect protein oligomerization.

Sites not aligning to the query:

43 R → L: in SMDP3; uncertain significance
53 N→Q: Does not affect N-glycosylation. Does not affect protein expression. Does not affect lamellar body membrane location.
101 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; dbSNP:rs121909182
124 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-140. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
140 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N → H: in dbSNP:rs45447801; N→Q: Loss of N-glycosylation. Reduces protein expression by 50%. Affects anterograde trafficking; when associated with Q-124. Reduces protein expression by 85%; when associated with Q-140. Does not affect lamellar body membrane location.
173:174 LK→AA: Loss of proteolytic processing.
174:175 Cleavage; by CTSL
215 Q → K: in SMDP3; loss of lamellar bodies membrane location; loss of proteolytic cleavage; increases cellular free cholesterol and phosphatidylcholine transport; loss of vesicles formation; increases free cholesterol induced cell death; loss of protein oligomerization; dbSNP:rs879159551
280 R → C: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs201299260
288 R → K: in SMDP3; uncertain significance; does not affect protein oligomerization; dbSNP:rs117603931
290 L → M: in a breast cancer sample; somatic mutation
292 E → V: in SMDP3; uncertain significance; does not affect lamellar bodies membrane location; does not affect proteolytic cleavage; affects lamellar bodies formation; does not affect cholesterol and phosphatidylcholine transport; decreases vesicles formation; does not affect free cholesterol induced cell death; dbSNP:rs149989682
766 P → S: in dbSNP:rs45592239
801 E → D: in a breast cancer sample; somatic mutation
945 N→Q: Does not affect lamellar body membrane location. Does not affect protein expression. Does not affect proteolytic processing.
982 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs1402761450
1069 H → Q: in a breast cancer sample; somatic mutation
1076 N → K: in SMDP3; uncertain significance; dbSNP:rs2093663770
1221 G → S: in SMDP3; does not affect intracellular vesicle membrane location; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; G→A: Decreases ATP hydrolysis activity of 15% compared to the wild-type.; G→T: Decreases ATP hydrolysis activity of 36% compared to the wild-type.; G→V: Decreases ATP hydrolysis activity of 18% compared to the wild-type.
1302 G → E: in SMDP3; uncertain significance; dbSNP:rs2093657978
1388 K → N: in SMDP3; decreases phosphatidylcholine transport; increases protein abundance; does not affect folding in the endoplasmic reticulum; decreases proteolytic processing; affects lamellar bodies development; reduces free cholesterol transport
1553 L → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs121909183
1580 L → P: in SMDP3; does not affect location in intracellular vesicle membrane; does not affect proteolytic cleavage; does not affect N-glycosylation; loss of ATP hydrolysis activity; decreases ATP binding in vitro; affects the intracellular vesicles development; decreases phosphatidylcholine transport; L→A: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→F: Decreases ATP hydrolysis activity of 13% compared to the wild-type.; L→V: Decreases ATP hydrolysis activity of 56% compared to the wild-type.
1591 Q → P: in SMDP3; loss of intracellular vesicle membrane location; loss of proteolytic cleavage; does not affect N-glycosylation; dbSNP:rs28936691

Q9DC29 ATP-binding cassette sub-family B member 6; ABC-type heme transporter ABCB6; EC 7.6.2.5 from Mus musculus (Mouse) (see paper)
32% identity, 86% coverage: 19:249/269 of query aligns to 578:817/842 of Q9DC29

query
sites
Q9DC29

P

P

A
x
G

E

A

G

G

P

P

-

L

-

R

-

F

-

H

-

K

A

G

A

R

L

I

S

E

V

F

E

E

G

N

V

V

S

H

H

F

S

S

Y

Y

G

A

K

D

-

G

R

Q

K

E

A

T

L

L

D

Q

N

D

V

V

S

S

F

F

S

T

V

V

K

M

P

P

S

G

S

Q

F

T

T

V

V

A

L

L

L

V

G

G

L

P

N

S

G

G

A

A

G

G

K

K

S

S

T

T

L

I

F

L

S

R

L

L

I

L

T

F

R

R

L

F

Y

Y

S

D

T

I

Q

S

T

S

G

G

K

C

I

I

K

R

I

I

F

D

G

G

Y

Q

E

D

V

I

G

S

Q

Q

D

V

P

T

G

Q

E

I

A

S

L

L

R

R

-

S

R

H

L

I

G

G

V

V

F

P

Q

Q

A

D

R

T

T

V

L

L

D

F

M

N

D

D

L

-

S

T

I

I

Q

A

Q

N

N

N

L

I

L

R

Y

Y

H

G

A

R

A

V

L

T

H

A

G

G

I

D

G

S

A

E

A

V

E

E

T

A

K

A

A

A

R

Q

A

A

A

G

V

I

L

H

E

D

Q

A

I

I

-

L

-

S

-

F

-

P

-

E

A

G

M

Y

T

E

D

T

R

Q

T

V

K

G

D

E

K

R

V

G

R

L

D

K

L

L

S

S

G

G

Q

E

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

T

L

I

L

L

H

K

H

A

P

P

Q

D

M

I

L

I

L

L

D

D

E

E

P

A

T

T

V

S

G

A

L

L

D

D

I

T

K

S

A

N

R

E

A

R

D

A

I

I

L

Q

K

A

H

S

V

L

R

A

D

K

L

V

V

C

A

T

H

N

S

R

G

T

I

T

G

I

V

V

L

I

W

-

T

-

T

A

H

H

L

R

I

L

D

S

E

T

V

V

N

V

P

N

D

A

D

D

D

Q

V

I

V

L

V

V

L

I

H

K

Q

D

G

G

K

C

I

I

L

I

D

E

K

R

G

G

R

R

V

H

S

E

D

A

V

L

A

S

R

R

A

G

G

G

V

V

G579 (≠ A20) mutation to E: Results in retention of the protein in the Golgi apparatus.

Sites not aligning to the query:

170 S→G: Results in retention of the protein in the Golgi apparatus.
356 L→P: Results in retention of the protein in the Golgi apparatus.

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
33% identity, 73% coverage: 26:221/269 of query aligns to 2:201/219 of 8w6iD

query
sites
8w6iD

L

I

S

R

V

F

E

E

G

H

V

V

S

S

H

K

S

A

Y
|
Y

-

L

G

G

K

G

R

R

K

Q

A

A

L

L

D

Q

N

G

V

V

S

T

F

F

S

H

V

M

K

Q

P

P

S

G

S

E

F

M

T

A

V

F

L

L

L

T

G

G

L

H

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

S

K

L

L

I

I

T

C

R

G

L

I

Y

E

S

R

T

P

Q

S

T

A

G

G

K

K

I

I

K

W

I

F

F

S

G

G

Y

H

E

D

V

I

G

T

Q

R

D

L

P

K

G

N

E

R

A

E

L

V

-

P

-

F

-

L

-

R

R

R

R

Q

L

I

G

G

V

M

V

I

F

F

Q
|
Q

A

D

R

H

T

H

L

L

D

L

M

M

D

D

L

R

S

T

I

V

Q

Y

Q

D

N

N

L

V

L

A

Y

I

H

P

A

L

A

I

L

I

H

A

G

G

I

A

G

S

A

G

A

D

E

D

T

I

K

R

A

R

R

R

A

V

A

S

A

A

V

A

L

L

E

D

Q

K

I

V

A

G

M

L

T

L

D

D

R
x
K

T

A

K

K

D

N

K

F

V

P

R

I

D
x
Q

L

L

S
|
S

G
|
G

Q
x
E

M

Q

R

Q

R

R

V

V

E

G

I

I

A

A

R

R

A

A

L

V

H

N

H

K

P

P

Q

A

M

V

L

L

L

A

D

D

E

E

P

P

T

T

V

G

G

N

L

L

D

D

I

D

K

A

A

L

R

S

A

E

D

G

I

I

L

L

K

R

H

L

V

F

R

E

D

E

L

F

V

-

A

N

H

R

S

V

G

G

I

V

G

T

V

V

L

L

W

M

T

A

T

T

H
|
H

L

D

I

I

D

N

E

L

V

I

N

S

binding phosphothiophosphoric acid-adenylate ester: Y11 (= Y35), S37 (≠ N60), G38 (= G61), A39 (= A62), G40 (= G63), K41 (= K64), S42 (= S65), T43 (= T66), Q86 (= Q105), K130 (≠ R149), Q137 (≠ D156), S139 (= S158), G140 (= G159), G141 (= G160), E142 (≠ Q161), H195 (= H215)

P0A9R7 Cell division ATP-binding protein FtsE from Escherichia coli (strain K12) (see paper)
33% identity, 73% coverage: 26:221/269 of query aligns to 2:201/222 of P0A9R7

query
sites
P0A9R7

L

I

S

R

V

F

E

E

G

H

V

V

S

S

H

K

S

A

Y

Y

-

L

G

G

K

G

R

R

K

Q

A

A

L

L

D

Q

N

G

V

V

S

T

F

F

S

H

V

M

K

Q

P

P

S

G

S

E

F

M

T

A

V

F

L

L

L

T

G

G

L

H

N

S

G

G

A

A

G

G

K
|
K

S

S

T

T

L

L

F

L

S

K

L

L

I

I

T
x
C

R

G

L

I

Y

E

S

R

T

P

Q

S

T

A

G

G

K

K

I

I

K

W

I

F

F

S

G

G

Y

H

E

D

V

I

G

T

Q

R

D

L

P

K

G

N

E

R

A

E

L

V

-

P

-

F

-

L

-

R

R

R

R

Q

L

I

G

G

V

M

V

I

F

F

Q

Q

A

D

R

H

T

H

L

L

D

L

M

M

D

D

L

R

S

T

I

V

Q

Y

Q

D

N

N

L

V

L

A

Y

I

H

P

A

L

A

I

L

I

H

A

G

G

I

A

G

S

A

G

A

D

E

D

T

I

K

R

A

R

R

R

A

V

A

S

A

A

V

A

L

L

E

D

Q

K

I

V

A

G

M

L

T

L

D

D

R

K

T

A

K

K

D

N

K

F

V

P

R

I

D

Q

L

L

S

S

G

G

Q

E

M

Q

R

Q

R

R

V

V

E

G

I

I

A

A

R

R

A

A

L

V

H

N

H

K

P

P

Q

A

M

V

L

L

L

A

D

D

E

E

P

P

T

T

V

G

G

N

L

L

D

D

I

D

K

A

A

L

R

S

A

E

D

G

I

I

L

L

K

R

H

L

V

F

R

E

D

E

L

F

V

-

A

N

H

R

S

V

G

G

I

V

G

T

V

V

L

L

W

M

T

A

T

T

H

H

L

D

I

I

D

N

E

L

V

I

N

S

K41 (= K64) mutation to R: Does not bind ATP.
C49 (≠ T72) mutation to A: Prevents dimer formation. Does not alter ATP-binding.

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
30% identity, 87% coverage: 26:259/269 of query aligns to 3:239/241 of 4u00A

query
sites
4u00A

L

I

S

R

V

I

E

R

G

N

V

L

S

H

H

K

S

W

Y
x
F

G

G

K

P

R

L

K

H

A
x
V

L

L

D

K

N

G

V

I

S

H

F

L

S

E

V

V

K

A

P

P

S

G

S

E

F

K

T

L

V

V

L

I

G

G

L

P

N
x
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

I

S

R

L

T

I

I

T

N

R

R

L

L

Y

E

S

D

T

F

Q

Q

T

E

G

G

K

E

I

V

K

V

I

V

F

D

G

G

Y

L

E

S

V

V

G

K

Q

D

D

D

P

R

G

A

-

L

-

R

E

E

A

I

L

R

R

R

R

E

L

V

G

G

V

M

V

V

F

F

Q

Q

A

Q

R

F

T

N

L

L

D

F

M

P

D

H

L

M

S

T

I

V

Q

L

Q

E

N

N

L

V

-

T

L

L

Y

A

H

P

A

M

A

R

L

V

H

R

G

R

I

W

G

P

A

R

A

E

E

K

T

A

K

E

A

K

R

K

A

A

A

L

A

E

V

L

L

L

E

E

Q

R

I

V

A

G

M

I

T

L

D

D

R

Q

T

A

K

R

D

K

K

Y

V

P

R

A

D

Q

L

L

S

S

G

G

Q

Q

M

Q

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

A

L

L

L

A

H

M

H

E

P

P

Q

K

M

I

L

M

L

L

L

F

D

D

E

E

P

P

T

T

V

S

G

A

L

L

D

D

I

P

K

E

A

M

R

V

A

G

D

E

I

V

L

L

K

D

H

V

V

M

R

R

D

D

L

L

V

-

A

A

H

Q

S

G

G

G

I

M

G

T

V

M

L

V

W

V

T

V

T

T

H

H

-

E

-

M

-

G

L

F

I

A

D

R

E

E

V

V

N

A

P

-

D

-

D

D

D

R

V

V

V

F

L

M

H

D

Q

G

G

G

K

Q

I

I

L

V

D

E

K

E

G

G

R

R

V

P

S

E

D

E

V

I

V

F

A

T

R

R

A

P

G

K

V

E

A

E

D

R

M

T

R

R

A

S

A

F

F

L

T

Q

K

R

L

V

binding adenosine-5'-diphosphate: F12 (≠ Y35), V17 (≠ A40), S37 (≠ N60), G38 (= G61), S39 (≠ A62), G40 (= G63), K41 (= K64), S42 (= S65), T43 (= T66)

3nh9A Nucleotide binding domain of human abcb6 (atp bound structure) (see paper)
33% identity, 90% coverage: 12:252/269 of query aligns to 16:266/273 of 3nh9A

query
sites
3nh9A

E

E

P

T

A

E

L

V

A

K

P

D

P

L

P

P

A

G

E

A

G

G

P

P

-

L

-

R

-

F

-

Q

-

K

A

G

A

R

L

I

S

E

V

F

E

E

G

N

V

V

S

H

H

F

S

S

Y
|
Y

G

A

K

D

-

G

R

R

K

E

A

T

L

L

D

Q

N

D

V

V

S

S

F

F

S

T

V

V

K

M

P

P

S

G

S

Q

F

T

T

L

V

A

L

L

L

V

G

G

L

P

N
x
S

G
|
G

A
|
A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

F

L

S
x
R

L

L

I

L

T

F

R

R

L

F

Y

Y

S

D

T

I

Q

S

T

S

G

G

K

C

I

I

K

R

I

I

F

D

G

G

Y

Q

E

D

V

I

G

S

Q

Q

D

V

P

T

G

Q

E

A

A

S

L

L

R

R

-

S

R

H

L

I

G

G

V

V

F

P

Q

Q

A

D

R

T

T

V

L

L

D

F

M

N

D

D

L

-

S

T

I

I

Q

A

Q

D

N

N

L

I

L

R

Y

Y

H

G

A

R

A

V

L

T

H

A

G

G

I

N

G

D

A

E

A

V

E

E

T

A

K

A

A

A

R

Q

A

A

A

G

V

I

L

H

E

D

Q

A

I

I

-

M

A

A

M

F

T

P

D

E

R

G

T

Y

K

R

D

T

K

Q

V

V

R

G

D

E

-

R

-

G

-

L

-

K

L

L

S

S

G

G

Q

E

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

T

L

I

L

L

H

K

H

A

P

P

Q

G

M

I

L

I

L

L

D

D

E

E

P

A

T

T

V

S

G

A

L

L

D

D

I

T

K

S

A

N

R

E

A

R

D

A

I

I

L

Q

K

A

H

S

V

L

R

A

D

K

L

V

V

C

A

A

H

N

S

R

G

T

I

T

G

I

V

V

L

V

W

-

T

-

T

A

H

H

L

R

I

L

D

S

E

T

V

V

N

V

P

N

D

A

D

D

D

Q

V

I

V

L

V

V

L

I

H

K

Q

D

G

G

K

C

I

I

L

V

D

E

K

R

G

G

R

R

V

H

S

E

D

A

V

L

A

S

R

R

A

G

G

G

V

V

-

Y

A

A

D

D

M

M

binding adenosine-5'-triphosphate: Y44 (= Y35), S70 (≠ N60), G71 (= G61), A72 (= A62), G73 (= G63), K74 (= K64), S75 (= S65), T76 (= T66), R79 (≠ S69)

6mjpA Lptb(e163q)fgc from vibrio cholerae (see paper)
33% identity, 84% coverage: 24:249/269 of query aligns to 1:229/240 of 6mjpA

query
sites
6mjpA

A

A

A

I

L

L

S

K

V

A

E

Q

G

H

V

L

S

A

H

K

S

S

Y

Y

G

K

K

K

R

R

K

K

A

V

L

V

D

S

N

D

V

V

S

S

F

L

S

Q

V

V

K

E

P

S

S

G

S

Q

F

I

T

V

V

G

L

L

G

G

L

P

N

N

G

G

A

A

G

G

K

K

S

T

T

T

L

S

F

F

S

Y

L

M

I

I

T

V

R

G

L

L

Y

V

S

A

T

R

Q

D

T

E

G

G

K

T

I

I

K

T

I

I

F

D

G

D

Y

N

E

D

V

I

G

S

Q

I

D

L

P

P

G

M

E

H

A

S

L

R

R

S

R

R

L

M

G

G

V

I

V

G

F

Y

-

L

-

P

Q

Q

A

E

R

A

T

S

L

I

D

F

M

R

D

K

L

L

S

S

I

V

Q

E

Q

D

N

N

L

I

L

M

Y

-

H

-

A

A

A

V

L

L

H

Q

G

T

-

R

-

E

I

L

G

T

A

H

A
x
E

E

E

T

R

K

Q

A
x
D

R

K

A

L

A

E

A

D

V

L

L

L

E

E

Q

E

I

F

A

H

M

I

T

Q

D

H

R

I

T

R

K

K

D

S

K

A

V

G

R

M

D

A

L

L

S

S

G

G

Q

E

M

R

R

R

V

V

E

E

I

I

A

A

R

R

A

A

L

L

L

A

H

A

H

N

P

P

Q

Q

M

F

L

I

L

L

D

D

E

Q

P

P

T

F

V

A

G

G

L

V

D

D

I

P

K

I

A

S

R

V

A

I

D

D

-

I

-

K

I

I

L

I

K

E

H

H

V

L

R

R

D

D

L

-

V

-

A

-

H

-

S

R

G

G

I

L

G

G

V

V

L

L

W

I

T

T

T

D

H

H

L

N

I

V

D

R

E

E

-

T

V

L

N

D

P

V

D

C

D

E

D

K

V

A

V

Y

V

I

L

V

H

S

Q

Q

G

G

K

R

I

L

L

I

D

A

K

E

G

G

R

T

V

P

S

Q

D

D

V

V

V

L

A

N

R

N

A

E

G

Q

V

V

binding calcium ion: E111 (≠ A131), D115 (≠ A135)

7w02A Cryo-em structure of atp-bound abca3 (see paper)
31% identity, 75% coverage: 38:238/269 of query aligns to 511:710/1566 of 7w02A

query
sites
7w02A

R

R

K

A

A

A

L

V

D

R

N

D

V

L

S

N

F

L

S

N

V

L

K

Y

P

E

S

G

S

Q

F

I

T

T

V

V

L

L

G

G

L

H

N
|
N

G
|
G

A

A

G
|
G

K
|
K

S
x
T

T
|
T

L

T

F

L

S

S

L

M

I

L

T

T

R

G

L

L

Y

F

S

P

T

P

Q

T

T

S

G

G

K

R

I

A

K

Y

I

I

F

S

G

G

Y

Y

E

E

V

I

G

S

Q

Q

D

D

P

M

G

V

E

Q

A

I

L

R

R

K

R

S

L

L

G

G

V

L

V

C

F

P

Q
|
Q

A

H

R

D

T

I

L

L

D

F

M

D

D

N

L

L

S

T

I

V

Q

A

Q

E

N

H

L

L

L

Y

Y

F

H

Y

A

A

A

Q

L

L

H

K

G

G

I

L

G

S

A

R

A

Q

E

K

T

C

K

P

A

E

R

E

A

V

A

K

A

Q

V

M

L

L

E

H

Q

I

I

I

A

G

M

L

T

E

D

D

R

K

T

W

K

N

D

S

K

R

V

S

R

R

D

F

L
|
L

S
|
S

G

G

Q

M

M

R

R

R

R

K

V

L

E

S

I

I

A

G

R

I

A

A

L

L

L

I

H

A

H

G

P

S

Q

K

M

V

L

L

L

I

L

L

D

D

E

Q

P

P

T

T

V

S

G

G

L

M

D

D

I

A

K

I

A

S

R

R

A

-

D

-

I

-

L

-

K

R

H

A

V

I

R

W

D

D

L

L

V

L

A

Q

H

R

-

Q

-

K

S

S

G

D

I

R

G

T

V

I

L

V

W

L

T

T

H

H

L

F

I

M

D

D

E

E

V

A

N

D

-

L

P

L

D

G

D

D

D

R

V

I

V

A

V

I

L

M

H

A

Q

K

G

G

K

E

I

L

L

Q

D

C

K

C

G

G

binding adenosine-5'-triphosphate: N533 (= N60), G534 (= G61), G536 (= G63), K537 (= K64), T538 (≠ S65), T539 (= T66), Q578 (= Q105), L630 (= L157), S631 (= S158)
binding magnesium ion: T538 (≠ S65), Q578 (= Q105)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 504, 1277, 1305, 1306, 1307, 1308, 1309, 1310, 1344, 1395, 1398
binding magnesium ion: 1310, 1344

Q61102 Iron-sulfur clusters transporter ABCB7, mitochondrial; ATP-binding cassette sub-family B member 7, mitochondrial; ATP-binding cassette transporter 7; ABC transporter 7 protein from Mus musculus (Mouse) (see paper)
32% identity, 86% coverage: 7:238/269 of query aligns to 451:688/752 of Q61102

query
sites
Q61102

D

D

T

T

T

R

V

I

A

K

E

D

P

K

A

V

L

M

A

A

P

P

P

L

A

Q

E

I

G

T

P

P

-

Q

-

T

A

A

A

T

L

V

S

A

V

F

E

D

G

N

V

V

S

H

H

F

S

E

Y

Y

G

I

K

E

-

G

R

Q

K

K

A

V

L

L

D

N

N

G

V

V

S

S

F

F

S

E

V

V

K

P

P

A

S

G

S

K

F

K

T

V

V

A

L

I

L

V

G

G

L

G

N

S

G

G

A

S

G

G

K

K

S

S

T

T

L

I

F

V

S

R

L

L

I

L

T

F

R

R

L

F

Y

Y

S

E

T

P

Q

Q

T

K

G

G

K

S

I

I

K

Y

I

L

F

A

G

G

Y

Q

E

N

V

L

G

Q

Q

D

D

V

P

S

G

L

E

E

A

S

L

L

R

R

-

A

L

V

G

G

V

V

F

P

Q

Q

A

-

R

-

T

-

L

-

D

-

M

-

D

D

L

A

S

V

I

L

Q

F

Q

H

N

N

L

T

L

I

Y

Y

H

Y

A

N

A

L

L

L

H

Y

G

G

-

N

I

I

G

N

A

A

A

S

E

P

T

E

K

E

A

V

R

Y

A

A

A

V

A

A

V

K

L

L

E

A

Q

G

I

L

-

H

-

D

-

A

-

I

-

L

-

R

-

M

-

P

-

H

A

G

M

Y

T

D

D

T

R

Q

T

V

K

G

D

E

K

R

V

G

R

L

D

K

L

L

S

S

G

G

Q

E

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

A

L

I

L

L

H

K

H

N

P

P

Q

P

M

V

L

I

L

L

L

Y

D

D

E

E

P

A

T

T

V

S

G

S

L

L

D

D

I

S

K

I

A

T

R

E

A

E

D

T

I

I

L

L

K

G

H

A

V

M

R

R

D

D

L

V

V

V

A

K

H

H

S

R

G

-

I

-

G

T

V

S

L

I

W

F

T

I

T

A

H

H

L

R

I

L

D

S

E

T

V

V

N

V

P

D

D

A

D

D

D

E

V

I

V

V

L

L

H

S

Q

Q

G

G

K

K

I

V

L

A

D

E

K

R

G

G

Sites not aligning to the query:

433 E→K: Exhibits siderocytosis with increased zinc protoporphyrin.

8hd0A Cell divisome spg hydrolysis machinery ftsex-envc
33% identity, 73% coverage: 26:221/269 of query aligns to 2:201/218 of 8hd0A

query
sites
8hd0A

L

I

S

R

V

F

E

E

G

H

V

V

S

S

H

K

S

A

Y
|
Y

-

L

G

G

K

G

R
|
R

K

Q

A
|
A

L

L

D

Q

N

G

V

V

S

T

F

F

S

H

V

M

K

Q

P

P

S

G

S

E

F

M

T

A

V

F

L

L

L

T

G

G

L

H

N

S

G

G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

F

L

S

K

L

L

I

I

T

C

R

G

L

I

Y

E

S

R

T

P

Q

S

T

A

G

G

K

K

I

I

K

W

I

F

F

S

G

G

Y

H

E

D

V

I

G

T

Q

R

D

L

P

K

G

N

E

R

A

E

L

V

-

P

-

F

-

L

-

R

R

R

R

Q

L

I

G

G

V

M

V

I

F

F

Q

Q

A

D

R

H

T

H

L

L

D

L

M

M

D

D

L

R

S

T

I

V

Q

Y

Q

D

N

N

L

V

L

A

Y

I

H

P

A

L

A

I

L

I

H

A

G

G

I

A

G

S

A

G

A

D

E

D

T

I

K

R

A

R

R

R

A

V

A

S

A

A

V

A

L

L

E

D

Q

K

I

V

A

G

M

L

T

L

D

D

R

K

T

A

K

K

D

N

K

F

V

P

R

I

D

Q

L

L

S
|
S

G

G

G
|
G

Q

E

M

Q

R

Q

R

R

V

V

E

G

I

I

A

A

R

R

A

A

L

V

H

N

H

K

P

P

Q

A

M

V

L

L

L

A

D

D

E

Q

P

P

T

T

V

G

G

N

L

L

D

D

I

D

K

A

A

L

R

S

A

E

D

G

I

I

L

L

K

R

H

L

V

F

R

E

D

E

L

F

V

-

A

N

H

R

S

V

G

G

I

V

G

T

V

V

L

L

W

M

T

A

T

T

H

H

L

D

I

I

D

N

E

L

V

I

N

S

binding adenosine-5'-triphosphate: Y11 (= Y35), R15 (= R38), A17 (= A40), G40 (= G63), K41 (= K64), S42 (= S65), T43 (= T66), S139 (= S158), G141 (= G160)

8k7bA Post-occluded structure of human abcb6 w546a mutant (adp/vo4-bound) (see paper)
33% identity, 93% coverage: 4:252/269 of query aligns to 331:584/590 of 8k7bA

query
sites
8k7bA

V

L

P

K

Q

E

D

E

T

T

T

E

V

V

A

K

E

D

P

L

A

P

L

G

A

A

P

G

P

P

P

L

A

R

E

F

G

Q

P

K

A

G

A

R

L

I

S

E

V

F

E

E

G

N

V

V

S

H

H

F

S

S

Y
|
Y

G

A

K

D

-

G

R

R

K

E

A

T

L

L

D

Q

N

D

V

V

S

S

F

F

S

T

V

V

K

M

P

P

S

G

S

Q

F

T

T

L

V

A

L

L

L

V

G

G

L

P

N

S

G
|
G

A

A

G
|
G

K
|
K

S
|
S

T
|
T

L

I

F

L

S

R

L

L

I

L

T

F

R

R

L

F

Y

Y

S

D

T

I

Q

S

T

S

G

G

K

C

I

I

K

R

I

I

F

D

G

G

Y

Q

E

D

V

I

G

S

Q

Q

D

V

P

T

G

Q

E

A

A

S

L

L

R

R

-

S

R

H

L

I

G

G

V

V

F

P

Q
|
Q

A

D

R

T

T

V

L

L

D

F

M

N

D

D

L

-

S

T

I

I

Q

A

Q

D

N

N

L

I

L

R

Y

Y

H

G

A

R

A

V

L

T

H

A

G

G

I

N

G

D

A

E

A

V

E

E

T

A

K

A

A

A

R

Q

A

A

A

G

V

I

L

H

E

D

Q

A

I

I

-

M

A

A

M

F

T

P

D

E

R

G

T

Y

K

R

D

T

K

Q

V

V

R

G

D

E

-

R

-

G

-

L

-
x
K

L
|
L

S
|
S

G
|
G

G

G

Q

E

M

K

R

Q

R

R

V

V

E

A

I

I

A

A

R

R

A

T

L

I

L

L

H

K

H

A

P

P

Q

G

M

I

L

I

L

L

D

D

E

E

P

A

T

T

V

S

G

A

L

L

D

D

I

T

K

S

A

N

R

E

A

R

D

A

I

I

L

Q

K

A

H

S

V

L

R

A

D

K

L

V

V

C

A

A

H

N

S

R

G

T

I

T

G

I

V

V

L

V

W

-

T

-

T

A

H
|
H

L

R

I

L

D

S

E

T

V

V

N

V

P

N

D

A

D

D

D

Q

V

I

V

L

V

V

L

I

H

K

Q

D

G

G

K

C

I

I

L

V

D

E

K

R

G

G

R

R

V

H

S

E

D

A

V

L

A

S

R

R

A

G

G

G

V

V

-

Y

A

A

D

D

M

M

binding adp orthovanadate: Y362 (= Y35), G389 (= G61), G391 (= G63), K392 (= K64), S393 (= S65), T394 (= T66), K489 (vs. gap), L490 (= L157), S491 (= S158), G492 (= G159), H546 (= H215)
binding magnesium ion: S393 (= S65), Q434 (= Q105)

P55339 ABC-type transporter ATP-binding protein EcsA from Bacillus subtilis (strain 168) (see paper)
29% identity, 84% coverage: 26:251/269 of query aligns to 4:229/247 of P55339

query
sites
P55339

L

L

S

S

V

V

E

K

G

D

V

L

S

T

H

G

S

G

Y

Y

G

T

K

R

R

N

K

P

A

V

L

L

D

K

N

N

V

V

S

S

F

F

S

T

V

L

K

E

P

P

S

N

S

Q

F

I

T

V

V

G

L

L

L

I

G

G

L

L

N

N

G

G

A

A

G

G

K

K

S

S

T

T

L

T

F

I

S

R

L

H

I

I

T

I

R

G

L

L

Y

M

S

D

T

P

Q

H

T

K

G

G

K

S

I

I

K

E

I

L

F

N

G

G

Y

K

E

T

V

F

G

A

Q

E

D

D

P

P

G

E

E

G

A

Y

L

R

R

S

R

Q

L

F

G

T

V

Y

V

I

F

P

Q

E

A

T

R

P

T

V

L

L

D

Y

M

E

D

E

L

L

S

T

I

L

Q

M

Q

E

N

H

L

L

L

E

Y

L

H

T

A

A

A

M

L

A

H

Y

G

G

I

L

G

S

A

K

A

E

E

T

T

M

K

E

A

K

R

R

A

L

A

P

V

L

L

L

E

K

Q

E

I

F

A

R

M

M

T

E

D

K

R

R

T

L

K

K

D

W

K

F

V

P

R

A

D

H

L

F

S

S

G

K

G

G

Q

M

M

K

R

Q

R

K

V

V

E

M

I

I

A

M

R

C

A

A

L

F

L

L

H

A

H

E

P

P

Q

A

M

L

L

Y

L

I

D

D

E

E

P

P

T

F

V

L

G
|
G

L

L

D

D

I

P

K

L

A

A

R

I

A

N

D

A

I

L

L

L

K

E

H

R

V

M

R

N

D

E

L

-

V

A

A

K

H

K

S

G

G

G

I

A

G

S

V

V

L

L

W

M

T

S

T

T

H

H

L

I

I

L

D

A

E

T

V

A

N

E

-

R

P

Y

D

C

D

D

D

S

V

F

V

I

L

L

H

H

Q

N

G

G

K

E

I

V

L

R

D

A

K

R

G

G

R

T

V

L

S

S

D

E

V

L

V

R

A

E

R

Q

A

F

G

G

V

M

A

K

D

D

G164 (= G186) mutation to E: In ecsA-26; exoamylase and other exoenzymes decrease, competence- and sporulation-deficient phenotype.

Query Sequence

>WP_020174170.1 NCBI__GCF_000385335.1:WP_020174170.1
MSAVPQDTTVAEPALAPPPAEGPAALSVEGVSHSYGKRKALDNVSFSVKPSSFTVLLGLN
GAGKSTLFSLITRLYSTQTGKIKIFGYEVGQDPGEALRRLGVVFQARTLDMDLSIQQNLL
YHAALHGIGAAETKARAAAVLEQIAMTDRTKDKVRDLSGGQMRRVEIARALLHHPQMLLL
DEPTVGLDIKARADILKHVRDLVAHSGIGVLWTTHLIDEVNPDDDVVVLHQGKILDKGRV
SDVVARAGVADMRAAFTKLTGTGPMGGES

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory