SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020174512.1 NCBI__GCF_000385335.1:WP_020174512.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
48% identity, 99% coverage: 1:321/323 of query aligns to 1:317/319 of 5v6qB

query
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5v6qB

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active site: L96 (= L102), R230 (= R234), D254 (= D258), E259 (= E263), H277 (= H281)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (= V78), V100 (= V106), F148 (≠ L152), L150 (= L154), G151 (= G155), R152 (= R156), I153 (= I157), T172 (≠ G176), R173 (= R177), V201 (≠ A205), P202 (= P206), S206 (≠ E210), T207 (= T211), V228 (= V232), G229 (≠ A233), R230 (= R234), H277 (= H281), A279 (≠ G283)

5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
55% identity, 82% coverage: 58:321/323 of query aligns to 51:316/319 of 5v7nA

query
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5v7nA

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active site: L95 (= L102), R229 (= R234), D253 (= D258), E258 (= E263), H276 (= H281)
binding 2-keto-D-gluconic acid: G70 (= G77), V71 (= V78), G72 (= G79), R229 (= R234), H276 (= H281), S279 (= S284), R285 (= R290)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (= V78), V99 (= V106), L149 (= L154), G150 (= G155), R151 (= R156), I152 (= I157), T171 (≠ G176), R172 (= R177), V200 (≠ A205), P201 (= P206), S205 (≠ E210), T206 (= T211), V227 (= V232), G228 (≠ A233), R229 (= R234), H276 (= H281), A278 (≠ G283)

5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
55% identity, 82% coverage: 58:321/323 of query aligns to 50:315/318 of 5j23A

query
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5j23A

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active site: L94 (= L102), R228 (= R234), D252 (= D258), E257 (= E263), H275 (= H281)
binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (= V78), L148 (= L154), G149 (= G155), R150 (= R156), I151 (= I157), T170 (≠ G176), R171 (= R177), P200 (= P206), S204 (≠ E210), T205 (= T211), R228 (= R234)

5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
55% identity, 82% coverage: 58:321/323 of query aligns to 50:315/317 of 5v7gA

query
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5v7gA

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active site: L94 (= L102), R228 (= R234), D252 (= D258), E257 (= E263), H275 (= H281)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (= V78), V98 (= V106), F146 (≠ L152), L148 (= L154), G149 (= G155), R150 (= R156), I151 (= I157), T170 (≠ G176), R171 (= R177), V199 (≠ A205), P200 (= P206), S204 (≠ E210), T205 (= T211), V226 (= V232), G227 (≠ A233), R228 (= R234), H275 (= H281), A277 (≠ G283)
binding oxalate ion: G69 (= G77), V70 (= V78), G71 (= G79), R228 (= R234), H275 (= H281)

Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
43% identity, 97% coverage: 1:314/323 of query aligns to 1:312/313 of Q65CJ7

query
sites
Q65CJ7

M

M

S

E

E

A

F

I

D

G

L

V

L

L

M

M

P

M

A

C

F

P

L

M

T

S

D

T

F

Y

L

L

V

E

D

Q

G

E

L

L

S

D

K

K

N

R

H

F

T

K

L

L

H

F

K

R

L

Y

W

W

E

T

A

Q

P

P

D

A

P

Q

A

R

A

D

K

F

I

L

S

A

A

L

V

Q

K

A

D

E

R

S

V

I

R

R

A

A

L

V

I

V

S

-

G

-

G

G

I

N

G

S

R

N

F

A

K

G

V

A

T

D

A

A

D

E

F

L

I

I

A

D

Q

A

F

L

P

P

N

K

L

L

E

E

A

I

I

V

F

S

H

S

M

F

G

S

V

V

G

G

Y

L

D

D

L

K

V

V

D

D

A

L

A

I

Y

K

A

C

G

E

A

E

H

K

G

G

I

V

I

R

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L

L

D

T

E

D

D

D

V

V

A

A

D

D

I

L

A

A

M

I

A

G

L

L

L

I

L

L

D

A

A

V

T

L

R

R

Q

R

I

I

P

C

Q

E

S

C

D

D

A

K

F

Y

L

V

R

R

S

R

G

G

K

A

W

W

L

K

T

F

G

G

T

D

Y

F

P

K

L

L

T

T

A

T

T

K

L

F

R

S

G

G

R

K

T

R

M

V

G

G

I

I

L

I

G

G

L
|
L

G
|
G

R
|
R

I
|
I

G

G

K

L

A

A

I

V

A

A

K

E

R

R

A

A

E

E

A

A

F

F

D

D

L

C

K

P

I

I

A

S

Y

Y

H

F

G
x
S

R
|
R

T
x
S

Q

K

A

P

G

N

V

T

A

N

Y

Y

P

T

Y

Y

A

G

T

S

P

V

I

V

A

E

L

L

A

A

A

S

A

N

C

S

D

D

V

I

L

L

M

V

V

V

V

A

A

C

P

P

G

L

G

T

A

P

E

E

T

T

K

T

H

H

I

I

V

I

N

N

K

R

A

E

V

V

L

I

E

D

A

A

L

L

G

G

P

P

D

K

G

G

I

V

L

L

I

I

N

N

V
x
I

A

G

R

R

G

G

S

P

L

H

V

V

D

D

E

E

A

P

A

E

L

L

I

V

E

S

A

A

L

L

Q

V

T

E

G

G

K

R

I

L

L

G

A

G

A

A

G

G

L

L

D
|
D

V

V

F

F

E

E

F

R

E

E

P

P

K

E

V

V

P

P

Q

E

A

K

L

L

I

F

D

G

C

L

Q

E

N

N

T

V

V

V

L

L

P

P

H

H

V

V

G

G

S

S

A

G

T

T

H

V

Y

E

T

T

R

R

Q

K

A

V

M

M

A

A

Q

D

L

L

V

V

I

V

D

G

N

N

V

L

A

E

S

A

W

H

T

F

S

S

G

G

K

K

G

P

P

L

L

L

T

T

P

P

V

V

LGRI 152:155 (= LGRI 154:157) binding NADP(+)
SRS 174:176 (≠ GRT 176:178) binding NADP(+)
I230 (≠ V232) binding NADP(+)
D256 (= D258) binding NADP(+)

3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
43% identity, 96% coverage: 6:314/323 of query aligns to 4:310/311 of 3bazA

query
sites
3bazA

L

V

L

L

M

M

P

M

A

C

F

P

L

M

T

S

D

T

F

Y

L

L

V

E

D

Q

G

E

L

L

S

D

K

K

N

R

H

F

T

K

L

L

H

F

K

R

L

Y

W

W

E

T

A

Q

P

P

D

A

P

Q

A

R

A

D

K

F

I

L

S

A

A

L

V

Q

K

A

D

E

R

S

V

I

R

R

A

A

L

V

I

V

S

-

G

-

G

G

I

N

G

S

R

N

F

A

K

G

V

A

T

D

A

A

D

E

F

L

I

I

A

D

Q

A

F

L

P

P

N

K

L

L

E

E

A

I

I

V

F

S

H

S

M

F

G

S

V
|
V

G

G

Y

L

D

D

L

K

V

V

D

D

A

L

A

I

Y

K

A

C

G

E

A

E

H

K

G

G

I

V

I

R

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

D

T

E

D

D

D

V

V

A

A

D

D

I

L

A

A

M

I

A

G

L

L

L

I

L

L

D

A

A

V

T

L

R

R

Q

R

I

I

P

C

Q

E

S

C

D

D

A

K

F

Y

L

V

R

R

S

R

G

G

K

A

W

W

L

K

T

F

G

G

T

D

Y

F

P

K

L

L

T

T

A

T

T

K

L

F

R

S

G

G

R

K

T

R

M

V

G

G

I

I

L

I

G
|
G

L
|
L

G
|
G

R
|
R

I
|
I

G

G

K

L

A

A

I

V

A

A

K

E

R

R

A

A

E

E

A

A

F

F

D

D

L

C

K

P

I

I

A

S

Y

Y

H

F

G
x
S

R
|
R

T
x
S

Q

K

A

P

G

N

V

T

A

N

Y

Y

P

T

Y

Y

A

G

T

S

P

V

I

V

A

E

L

L

A

A

A

S

A

N

C

S

D

D

V

I

L

L

M

V

V

V

V

A

A
x
C

P
|
P

G

L

G

T

A

P

E

E

T
|
T

K

T

H

H

I

I

V

I

N

N

K

R

A

E

V

V

L

I

E

D

A

A

L

L

G

G

P

P

D

K

G

G

I

V

L

L

I

I

N

N

V
x
I

A
x
G

R
|
R

G

G

S

P

L

H

V

V

D

D

E

E

A

P

A

E

L

L

I

V

E

S

A

A

L

L

Q

V

T

E

G

G

K

R

I

L

L

G

A

G

A
|
A

G

G

L

L

D
|
D

V

V

F

F

E

E

F

R

E
|
E

P

P

K

E

V

V

P

P

Q

E

A

K

L

L

I

F

D

G

C

L

Q

E

N

N

T

V

V

V

L

L

P

P

H
|
H

V

V

G
|
G

S

S

A

G

T

T

H

V

Y

E

T

T

R

R

Q

K

A

V

M

M

A

A

Q

D

L

L

V

V

I

V

D

G

N

N

V

L

A

E

S

A

W

H

T

F

S

S

G

G

K

K

G

P

P

L

L

L

T

T

P

P

V

V

active site: L98 (= L102), R230 (= R234), A251 (= A255), D254 (= D258), E259 (= E263), H277 (= H281)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V78), G149 (= G153), L150 (= L154), G151 (= G155), R152 (= R156), I153 (= I157), S172 (≠ G176), R173 (= R177), S174 (≠ T178), C201 (≠ A205), P202 (= P206), T207 (= T211), I228 (≠ V232), G229 (≠ A233), R230 (= R234), D254 (= D258), H277 (= H281), G279 (= G283)

5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
39% identity, 80% coverage: 58:314/323 of query aligns to 56:321/334 of 5aovA

query
sites
5aovA

K

R

V

I

T

D

A

Q

D

E

F

V

I

F

A

E

Q

N

F

A

P

P

N

R

L

L

E

R

A

I

I

V

F

A

H

N

M
x
Y

G
x
A

V
|
V

G
|
G

Y

Y

D

D

L

N

V

I

D

D

A

V

A

E

Y

E

A

A

G

T

A

R

H

R

G

G

I

I

I

Y

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

D

T

E

N

D

A

V
x
T

A

A

D

D

I

H

A

A

M

F

A

A

L

L

D

A

A

T

T

A

R

R

Q

H

I

V

P

V

Q

K

S

G

D

D

A

K

F

F

L

V

R

R

S

S

G

G

K

E

W

W

L

K

T

R

G

K

-

G

-

I

-

A

-

W

-

H

-

P

T

K

Y

W

P

F

L

L

T

G

A

Y

T

E

L

L

R

Y

G

G

R

K

T

T

M

I

G

G

I

I

L

V

G

G

L
x
F

G
|
G

R
|
R

I
|
I

G

G

K

Q

A

A

I

I

A

A

K

R

R

R

A

A

E

K

A

G

F

F

D

N

L

M

K

R

I

I

A

L

Y

Y

H

Y

G
x
S

R
|
R

T

T

Q

R

Q

K

A

S

G

Q

V

A

A

E

Y

K

P

E

Y

L

-

G

-

A

-

E

Y

Y

A

R

T

P

P

L

I

E

A

E

L

V

A

L

A

K

A

E

C

S

D

D

V

F

L

V

M

I

V

L

V
x
A

A
x
V

P
|
P

G

L

G

T

A

K

E

E

T
|
T

K

M

H

Y

I

M

V

I

N

N

K

E

A

E

V

R

L

L

E

K

A

L

L

M

G

K

P

P

D

T

G

A

I

I

L

L

I

V

N

N

V
x
I

A
|
A

R
|
R

G

G

S

K

L

V

V

V

D

D

E

T

A

K

A

A

L

L

I

I

E

K

A

A

L

L

Q

K

T

E

G

G

K

W

I

I

L

A

A

G

A

A

G

G

L

L

D
|
D

V

V

F

F

E

E

F

E

E
|
E

P

P

K

Y

V

Y

P

N

Q

E

A

E

L

L

I

F

D

S

C

L

Q

D

N

N

T

V

V

V

L

L

L

T

P

P

H
|
H

V

I

G
|
G

S

S

A

A

T

T

H

F

Y

E

T

A

R

R

Q

E

A

A

M

M

A

A

Q

E

L

L

V

V

I

A

D

R

N

N

V

L

A

I

S

A

W

F

T

K

S

R

G

G

K

E

G

I

P

P

L

P

T

T

P

L

V

V

active site: L100 (= L102), R241 (= R234), D265 (= D258), E270 (= E263), H288 (= H281)
binding glyoxylic acid: Y74 (≠ M76), A75 (≠ G77), V76 (= V78), G77 (= G79), R241 (= R234), H288 (= H281)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V78), T104 (≠ V106), F158 (≠ L154), G159 (= G155), R160 (= R156), I161 (= I157), S180 (≠ G176), R181 (= R177), A211 (≠ V204), V212 (≠ A205), P213 (= P206), T218 (= T211), I239 (≠ V232), A240 (= A233), R241 (= R234), H288 (= H281), G290 (= G283)

Sites not aligning to the query:

binding glyoxylic acid: 52, 53, 53

6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
41% identity, 80% coverage: 58:314/323 of query aligns to 55:320/332 of 6biiA

query
sites
6biiA

K

K

V

I

T

D

A

R

D

E

F

V

I

F

A

D

Q

A

F

A

P

P

N

R

L

L

E

R

A

I

I

V

F

A

H

N

M

Y

G

A

V
|
V

G

G

Y

Y

D

D

L

N

V

I

D

D

A

I

A

E

Y

E

A

A

G

T

A

K

H

R

G

G

I

I

I

Y

V

V

T

T

N

N

T

T

P

P

G

D

V

V

L
|
L

D

T

E

D

D

A

V
x
T

A

A

D

D

I

L

A

A

M

W

A

A

L

L

D

A

A

A

T

A

R

R

Q

H

I

V

P

V

Q

K

S

G

D

D

A

K

F

F

L

V

R

R

S

S

G

G

K

E

W

W

L

K

T

R

-

R

-

G

-

I

G

A

T

W

Y

H

P

P

-

K

-

M

-

F

L

L

T

G

A

Y

T

D

L

V

R

Y

G

G

R

K

T

T

M

I

G

G

I

I

L

V

G
|
G

L
x
F

G
|
G

R
|
R

I
|
I

G

G

K

Q

A

A

I

I

A

A

K

K

R

R

A

A

E

K

A

G

F

F

D

G

L

M

K

R

I

I

A

L

Y

Y

H

T

G
x
A

R
|
R

T
x
S

Q

R

Q
x
K

A

P

G

E

V

A

A

E

Y

K

P

E

Y

L

Y

G

A

A

-

E

-

F

T

K

P

P

I

L

A

E

-

E

L

L

A

L

A

R

A

E

C

S

D

D

V

F

L

V

M

V

V

L

V

A

A
x
V

P
|
P

G

L

G

T

A

K

E
|
E

T
|
T

K

Y

H

H

I

M

V

I

N

N

K

E

A

E

V

R

L

L

E

R

A

L

L

M

G

K

P

P

D

T

G

A

I

V

L

L

I

V

N

N

V
|
V

A
|
A

R
|
R

G

G

S

K

L

V

V

V

D

D

E

T

A

K

A

A

L

L

I

I

E

R

A

A

L

L

Q

K

T

E

G

G

K

W

I

I

L

A

A

A

G

G

L

L

D
|
D

V

V

F

F

E

E

F

E

E
|
E

P

P

K

Y

V

Y

P

D

Q

E

A

E

L

L

I

F

D

A

C

L

Q

D

N

N

T

V

V

V

L

L

L

T

P

P

H
|
H

V

I

G
|
G

S

S

A

A

T

T

H

F

Y

G

T

A

R

R

Q

E

A

G

M

M

A

A

Q

E

L

L

V

V

I

A

D

K

N

N

V

L

A

I

S

A

W

F

T

K

S

N

G

G

K

E

G

V

P

P

L

P

T

T

P

L

V

V

active site: L99 (= L102), R240 (= R234), D264 (= D258), E269 (= E263), H287 (= H281)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V78), T103 (≠ V106), G156 (= G153), F157 (≠ L154), G158 (= G155), R159 (= R156), I160 (= I157), A179 (≠ G176), R180 (= R177), S181 (≠ T178), K183 (≠ Q180), V211 (≠ A205), P212 (= P206), E216 (= E210), T217 (= T211), V238 (= V232), A239 (= A233), R240 (= R234), D264 (= D258), H287 (= H281), G289 (= G283)

O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
40% identity, 80% coverage: 58:314/323 of query aligns to 56:321/334 of O58320

query
sites
O58320

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LGRI 158:161 (= LGRI 154:157) binding NADP(+)
SRT 180:182 (≠ GRT 176:178) binding NADP(+)
IAR 239:241 (≠ VAR 232:234) binding NADP(+)
HIG 288:290 (≠ HVG 281:283) binding NADP(+)

2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
40% identity, 80% coverage: 58:314/323 of query aligns to 56:321/333 of 2dbqA

query
sites
2dbqA

K

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active site: L100 (= L102), R241 (= R234), D265 (= D258), E270 (= E263), H288 (= H281)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V78), T104 (≠ V106), L158 (= L154), G159 (= G155), R160 (= R156), I161 (= I157), S180 (≠ G176), R181 (= R177), T182 (= T178), A211 (≠ V204), V212 (≠ A205), P213 (= P206), T218 (= T211), I239 (≠ V232), A240 (= A233), R241 (= R234), D265 (= D258), H288 (= H281), G290 (= G283)

O43175 D-3-phosphoglycerate dehydrogenase; 3-PGDH; 2-oxoglutarate reductase; Malate dehydrogenase; EC 1.1.1.95; EC 1.1.1.399; EC 1.1.1.37 from Homo sapiens (Human) (see 3 papers)
33% identity, 78% coverage: 58:308/323 of query aligns to 58:310/533 of O43175

query
sites
O43175

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T78 (≠ V78) binding NAD(+)
R135 (≠ T135) to W: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606949
RI 155:156 (= RI 156:157) binding NAD(+)
D175 (≠ G176) binding NAD(+)
T207 (≠ A205) binding NAD(+)
CAR 234:236 (≠ VAR 232:234) binding NAD(+)
D260 (= D258) binding NAD(+)
V261 (= V259) to M: in PHGDHD; results in a four-fold decrease in substrate affinity and a slight increase in maximal enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs267606947
HLGA 283:286 (≠ HVGS 281:284) binding NAD(+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
373 A → T: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs201553627
377 G → S: in PHGDHD; results in a 2-fold decrease in enzyme activity with 3-phosphohydroxypyruvate, but no change in substrate affinity; dbSNP:rs267606948
425 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907988
490 V → M: in PHGDHD; results in almost undetectable enzyme activity with 3-phosphohydroxypyruvate; dbSNP:rs121907987

6rj3A Crystal structure of phgdh in complex with compound 15 (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 52:283/297 of 6rj3A

query
sites
6rj3A

K

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binding 4-[(1~{R})-1-[(2-methyl-5-phenyl-pyrazol-3-yl)carbonylamino]ethyl]benzoic acid: L145 (= L152), Y168 (≠ H175), D169 (≠ G176), P170 (≠ R177), I171 (≠ T178), I172 (≠ Q179), H200 (≠ V204), T201 (≠ A205), P202 (= P206), L210 (≠ I214)

6rj2A Crystal structure of phgdh in complex with compound 40 (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 50:281/299 of 6rj2A

query
sites
6rj2A

K

K

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M

L

G

G

I

I

L

L

G

G

L

L

G
|
G

R

R

I
|
I

G

G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H
x
Y

G
x
D

R
x
P

T
x
I

Q
x
I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L

L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V
x
H

A
x
T

P

P

G

L

A

P

E

S

T

T

K

T

H

G

I
x
L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V

C

A

A

R
|
R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H

H

V

L

G

G

S

A

A

S

T

T

H

K

binding ~{N}-[(1~{R})-1-[4-(ethanoylsulfamoyl)phenyl]ethyl]-2-methyl-5-phenyl-pyrazole-3-carboxamide: G146 (= G155), I148 (= I157), Y166 (≠ H175), D167 (≠ G176), P168 (≠ R177), I169 (≠ T178), I170 (≠ Q179), H198 (≠ V204), T199 (≠ A205), L208 (≠ I214), R228 (= R234)

6cwaA Crystal structure phgdh in complex with nadh and 3-phosphoglycerate at 1.77 a resolution (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 52:283/299 of 6cwaA

query
sites
6cwaA

K

K

V

V

T

T

A

A

D

D

F

V

I

I

A

N

Q

A

F

A

P

E

N

K

L

L

E

Q

A

V

I

V

F

G

H

R

M

A

G

G

V

T

G

G

Y

V

D

D

L

N

V

V

D

D

A

L

A

E

Y

A

A

A

G

T

A

R

H

K

G

G

I

I

I

L

V

V

T

M

N

N

T

T

P

P

G

N

V

G

L
x
N

D

S

E

L

D

S

V
x
A

A

A

D

E

I

L

A

T

M

C

A

G

L

M

L

I

L

M

D

C

A

L

T

A

R

R

Q

Q

I

I

P

P

Q

Q

S

A

D

T

A

A

F

S

L

M

R

K

S

D

G

G

K

K

W

W

L

E

T

R

G

K

T

K

Y

F

P

-

L

M

T

G

A

T

T

E

L

L

R

N

G

G

R

K

T

T

M

L

G

G

I

I

L

L

G

G

L

L

G

G

R
|
R

I
|
I

G

G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H
x
Y

G
x
D

R
x
P

T
x
I

Q

I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L

L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V
x
H

A
x
T

P
|
P

G

L

A

P

E

S

T
|
T

K

T

H

G

I

L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V
x
C

A
|
A

R
|
R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H
|
H

V

L

G
|
G

S

A

A

S

T

T

H

K

binding 1,4-dihydronicotinamide adenine dinucleotide: N96 (≠ L102), A100 (≠ V106), R149 (= R156), I150 (= I157), Y168 (≠ H175), D169 (≠ G176), P170 (≠ R177), I171 (≠ T178), H200 (≠ V204), T201 (≠ A205), P202 (= P206), T207 (= T211), C228 (≠ V232), A229 (= A233), R230 (= R234), H277 (= H281), G279 (= G283)

7ewhA Crystal structure of human phgdh in complex with homoharringtonine (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 53:284/302 of 7ewhA

query
sites
7ewhA

K

K

V

V

T

T

A

A

D

D

F

V

I

I

A

N

Q

A

F

A

P

E

N

K

L

L

E

Q

A

V

I

V

F

G

H

R

M

A

G

G

V

T

G

G

Y

V

D

D

L

N

V

V

D

D

A

L

A

E

Y

A

A

A

G

T

A

R

H

K

G

G

I

I

I

L

V

V

T

M

N

N

T

T

P

P

G

N

V

G

L

N

D

S

E

L

D

S

V

A

A

A

D

E

I

L

A

T

M

C

A

G

L

M

L

I

L

M

D

C

A

L

T

A

R

R

Q

Q

I

I

P

P

Q

Q

S

A

D

T

A

A

F

S

L

M

R

K

S

D

G

G

K

K

W

W

L

E

T

R

G

K

T

K

Y

F

P

-

L

M

T

G

A

T

T

E

L

L

R

N

G

G

R

K

T

T

M

L

G

G

I

I

L
|
L

G
|
G

L
|
L

G
|
G

R
|
R

I
|
I

G
|
G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H

Y

G
x
D

R

P

T

I

Q

I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L

L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V
x
H

A
x
T

P
|
P

G

L

A

P

E

S

T

T

K

T

H

G

I

L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V

C

A

A

R

R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H

H

V

L

G

G

S

A

A

S

T

T

H

K

binding (3beta)-O~3~-[(2R)-2,6-dihydroxy-2-(2-methoxy-2-oxoethyl)-6-methylheptanoyl]cephalotaxine: L146 (= L152), G147 (= G153), L148 (= L154), G149 (= G155), R150 (= R156), I151 (= I157), G152 (= G158), D170 (≠ G176), H201 (≠ V204), T202 (≠ A205), P203 (= P206)

6rihA Crystal structure of phgdh in complex with compound 9 (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 53:284/302 of 6rihA

query
sites
6rihA

K

K

V

V

T

T

A

A

D

D

F

V

I

I

A

N

Q

A

F

A

P

E

N

K

L

L

E

Q

A

V

I

V

F

G

H

R

M

A

G

G

V

T

G

G

Y

V

D

D

L

N

V

V

D

D

A

L

A

E

Y

A

A

A

G

T

A

R

H

K

G

G

I

I

I

L

V

V

T

M

N

N

T

T

P

P

G

N

V

G

L

N

D

S

E

L

D

S

V

A

A

A

D

E

I

L

A

T

M

C

A

G

L

M

L

I

L

M

D

C

A

L

T

A

R

R

Q

Q

I

I

P

P

Q

Q

S

A

D

T

A

A

F

S

L

M

R

K

S

D

G

G

K

K

W

W

L

E

T

R

G

K

T

K

Y

F

P

-

L

M

T

G

A

T

T

E

L

L

R

N

G

G

R

K

T

T

M

L

G

G

I

I

L

L

G

G

L

L

G
|
G

R

R

I

I

G

G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H
x
Y

G
x
D

R
x
P

T
x
I

Q
x
I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L

L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V

H

A
x
T

P
|
P

G

L

A

P

E

S

T

T

K

T

H

G

I
x
L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V

C

A

A

R

R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H

H

V

L

G

G

S

A

A

S

T

T

H

K

binding ~{N}-cyclopropyl-2-methyl-5-phenyl-pyrazole-3-carboxamide: G149 (= G155), Y169 (≠ H175), D170 (≠ G176), P171 (≠ R177), I172 (≠ T178), I173 (≠ Q179), T202 (≠ A205), P203 (= P206), L211 (≠ I214)

7dkmA Phgdh covalently linked to oridonin (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 54:285/306 of 7dkmA

query
sites
7dkmA

K

K

V

V

T

T

A

A

D

D

F

V

I

I

A

N

Q

A

F

A

P

E

N

K

L

L

E

Q

A

V

I

V

F

G

H

R

M

A

G

G

V
x
T

G

G

Y

V

D

D

L

N

V

V

D

D

A

L

A

E

Y

A

A

A

G

T

A

R

H

K

G

G

I

I

I

L

V

V

T

M

N

N

T

T

P

P

G

N

V

G

L

N

D

S

E

L

D

S

V
x
A

A

A

D

E

I

L

A

T

M

C

A

G

L

M

L

I

L

M

D

C

A

L

T

A

R

R

Q

Q

I

I

P

P

Q

Q

S

A

D

T

A

A

F

S

L

M

R

K

S

D

G

G

K

K

W

W

L

E

T

R

G

K

T

K

Y

F

P

-

L

M

T

G

A

T

T

E

L

L

R

N

G

G

R

K

T

T

M

L

G

G

I

I

L

L

G
|
G

L

L

G

G

R
|
R

I
|
I

G

G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H
x
Y

G
x
D

R
x
P

T
x
I

Q

I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L

L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V
x
H

A
x
T

P
|
P

G

L

A

P

E

S

T
|
T

K

T

H

G

I

L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V
x
C

A
|
A

R
|
R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H
|
H

V

L

G
|
G

S

A

A

S

T

T

H

K

binding nicotinamide-adenine-dinucleotide: T74 (≠ V78), A102 (≠ V106), G148 (= G153), R151 (= R156), I152 (= I157), Y170 (≠ H175), D171 (≠ G176), P172 (≠ R177), I173 (≠ T178), H202 (≠ V204), T203 (≠ A205), P204 (= P206), T209 (= T211), C230 (≠ V232), A231 (= A233), R232 (= R234), H279 (= H281), G281 (= G283)

Sites not aligning to the query:

binding (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14R,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one: 14, 17, 18, 293

6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 53:284/303 of 6plgA

query
sites
6plgA

K

K

V

V

T

T

A

A

D

D

F

V

I

I

A

N

Q

A

F

A

P

E

N

K

L

L

E

Q

A

V

I

V

F

G

H

R

M

A

G

G

V

T

G

G

Y

V

D

D

L

N

V

V

D

D

A

L

A

E

Y

A

A

A

G

T

A

R

H

K

G

G

I

I

I

L

V

V

T

M

N

N

T

T

P

P

G

N

V

G

L

N

D

S

E

L

D

S

V

A

A

A

D

E

I

L

A

T

M

C

A

G

L

M

L

I

L

M

D

C

A

L

T

A

R

R

Q

Q

I

I

P

P

Q

Q

S

A

D

T

A

A

F

S

L

M

R

K

S

D

G

G

K

K

W

W

L

E

T

R

G

K

T

K

Y

F

P

-

L

M

T

G

A

T

T

E

L

L

R

N

G

G

R

K

T

T

M

L

G

G

I

I

L

L

G

G

L

L

G
|
G

R
|
R

I

I

G

G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H

Y

G
x
D

R
x
P

T
x
I

Q
x
I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L

L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V
x
H

A
x
T

P

P

G

L

G
x
L

A

P

E

S

T

T

K

T

H

G

I

L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V

C

A

A

R

R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H

H

V

L

G

G

S

A

A

S

T

T

H

K

binding (2S)-(4-{3-[(4,5-dichloro-1-methyl-1H-indole-2-carbonyl)amino]oxetan-3-yl}phenyl)(pyridin-3-yl)acetic acid: G149 (= G155), R150 (= R156), D170 (≠ G176), P171 (≠ R177), I172 (≠ T178), I173 (≠ Q179), H201 (≠ V204), T202 (≠ A205), L205 (≠ G208)

6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 54:285/305 of 6plfA

query
sites
6plfA

K

K

V

V

T

T

A

A

D

D

F

V

I

I

A

N

Q

A

F

A

P

E

N

K

L

L

E

Q

A

V

I

V

F

G

H

R

M

A

G

G

V

T

G

G

Y

V

D

D

L

N

V

V

D

D

A

L

A

E

Y

A

A

A

G

T

A

R

H

K

G

G

I

I

I

L

V

V

T

M

N

N

T

T

P

P

G

N

V

G

L

N

D

S

E

L

D

S

V

A

A

A

D

E

I

L

A

T

M

C

A

G

L

M

L

I

L

M

D

C

A

L

T

A

R

R

Q

Q

I

I

P

P

Q

Q

S

A

D

T

A

A

F

S

L

M

R

K

S

D

G

G

K

K

W

W

L

E

T

R

G

K

T

K

Y

F

P

-

L

M

T

G

A

T

T

E

L

L

R

N

G

G

R

K

T

T

M

L

G

G

I

I

L

L

G

G

L

L

G

G

R

R

I

I

G

G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H
x
Y

G
x
D

R
x
P

T

I

Q

I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L
|
L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V
x
H

A
x
T

P
|
P

G

L

A

P

E

S

T

T

K

T

H

G

I
x
L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V

C

A

A

R

R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H

H

V

L

G

G

S

A

A

S

T

T

H

K

binding 4-{(1S)-1-[(5-chloro-6-{[(5S)-2-oxo-1,3-oxazolidin-5-yl]methoxy}-1H-indole-2-carbonyl)amino]-2-hydroxyethyl}benzoic acid: Y170 (≠ H175), D171 (≠ G176), P172 (≠ R177), L189 (= L194), H202 (≠ V204), T203 (≠ A205), P204 (= P206), L212 (≠ I214)

6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
35% identity, 71% coverage: 58:287/323 of query aligns to 53:284/301 of 6rj5A

query
sites
6rj5A

K

K

V

V

T

T

A

A

D

D

F

V

I

I

A

N

Q

A

F

A

P

E

N

K

L

L

E

Q

A

V

I

V

F

G

H

R

M

A

G

G

V

T

G

G

Y

V

D

D

L

N

V

V

D

D

A

L

A

E

Y

A

A

A

G

T

A

R

H

K

G

G

I

I

I

L

V

V

T

M

N

N

T

T

P

P

G

N

V

G

L

N

D

S

E

L

D

S

V

A

A

A

D

E

I

L

A

T

M

C

A

G

L

M

L

I

L

M

D

C

A

L

T

A

R

R

Q

Q

I

I

P

P

Q

Q

S

A

D

T

A

A

F

S

L

M

R

K

S

D

G

G

K

K

W

W

L

E

T

R

G

K

T

K

Y

F

P

-

L

M

T

G

A

T

T

E

L

L

R

N

G

G

R

K

T

T

M

L

G

G

I

I

L
|
L

G

G

L

L

G

G

R

R

I

I

G

G

K

R

A

E

I

V

A

A

K

T

R

R

A

M

E

Q

A

S

F

F

D

G

L

M

K

K

I

T

A

I

Y

G

H
x
Y

G
x
D

R
x
P

T

I

Q
x
I

Q

S

A

P

G

E

V

V

A

S

Y

A

P

S

Y

F

Y

G

A

V

T

Q

P

Q

I

L

A

P

L

L

A

E

A

I

-

W

-

P

-

L

C

C

D

D

V

F

L

I

M

T

V

V

V
x
H

A
x
T

P
|
P

G

L

A

P

E

S

T

T

K

T

H

G

I
x
L

V

L

N

N

K

D

A

N

V

T

L

F

E

A

A

Q

L

C

G

K

P

K

D

G

G

V

I

R

L

V

I

V

N

N

V

C

A

A

R

R

G

G

S

G

L

I

V

V

D

D

E

E

A

G

A

A

L

L

I

L

E

R

A

A

L

L

Q

Q

T

S

G

G

K

Q

I

C

L

A

A

G

A

A

G

A

L

L

D

D

V

V

F

F

E

T

F

E

E

E

P

P

K

P

V

R

P

D

Q

R

A

A

L

L

I

V

D

D

C

H

Q

E

N

N

T

V

V

I

L

S

L

C

P

P

H

H

V

L

G

G

S

A

A

S

T

T

H

K

binding 2-methyl-~{N}-[(1~{R})-1-[4-(methylsulfonylcarbamoyl)phenyl]ethyl]-5-phenyl-pyrazole-3-carboxamide: L146 (= L152), Y169 (≠ H175), D170 (≠ G176), P171 (≠ R177), I173 (≠ Q179), H201 (≠ V204), T202 (≠ A205), P203 (= P206), L211 (≠ I214)

Query Sequence

>WP_020174512.1 NCBI__GCF_000385335.1:WP_020174512.1
MSEFDLLMPAFLTDFLVDGLSKNHTLHKLWEAPDPAAKISAVKDRVRALISGGIGRFKVT
ADFIAQFPNLEAIFHMGVGYDLVDAAYAGAHGIIVTNTPGVLDEDVADIAMALLLDATRQ
IPQSDAFLRSGKWLTGTYPLTATLRGRTMGILGLGRIGKAIAKRAEAFDLKIAYHGRTQQ
AGVAYPYYATPIALAAACDVLMVVAPGGAETKHIVNKAVLEALGPDGILINVARGSLVDE
AALIEALQTGKILAAGLDVFEFEPKVPQALIDCQNTVLLPHVGSATHYTRQAMAQLVIDN
VASWTSGKGPLTPVAETPWQRLN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory