SitesBLAST

Q58989 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see 3 papers)
41% identity, 65% coverage: 81:289/324 of query aligns to 3:211/211 of Q58989

query
sites
Q58989

R

K

K

K

L

L

L

I

L

L

A

F

D
|
D

M

F

D
|
D

S

S

T

T

V

L

I

V

G

N

Q

N

E
|
E

C

T

I

I

N

D

E

E

L

I

A

A

Q

R

E

E

M

A

G

G

K

V

R

E

A

E

Q

E

V

V

T

K

K

K

I

I

T

T

E

K

Q

E

A

A

M

M

A

E

G

G

E

K

I

L

D

N

F

F

E

E

T

Q

S

S

L

L

R

R

E

K

R
|
R

V

V

T

S

V

L

I

L

K

K

N

D

L

L

R

P

A

I

D

E

T

K

V

V

N

E

R

K

I

A

L

I

G

-

K

K

K

R

I

I

T

T

I

P

T

T

N

E

G

G

A

A

R

E

V

E

L

T

V

I

Q

K

T

E

M

L

R

K

A

-

N

N

R

R

A

G

Y

Y

T

V

V

V

L

A

V

V

T

V

S
|
S

G
|
G

-

G

F

F

S

D

A

I

F

A

A

V

A

N

P

K

V

I

T

K

E

E

K

K

V

L

G

G

F

L

N

D

E

Y

F

A

Q

F

A

A

N

N

V

R

L

L

G

I

V

V

A

K

E

D

N

G

R

K

F

L

T

T

G

G

L

D

L

V

E

E

E

G

P

E

V

V

L

L

G

K

T

E

E

N

A

A

K
|
K

R

G

D

E

I

I

L

L

I

E

R

K

L

I

R

A

E

K

K

I

E

E

F

G

L

I

P

N

K

L

V

E

Q

D

V

T

M

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D

D

L

I

A

S

M

M

L

F

A

K

E

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

H

C

A

A

K

K

P

P

A

I

V

L

A

K

A

E

A

K

A

A

D

D

A

I

R

C

I

I

D

E

H

K

A

R

D

D

L

L

T

R

A

E

L

I

L

L

-

K

Y

Y

L

I

Q

K

D11 (= D89) active site, Nucleophile; binding Mg(2+); mutation to N: Loss of activity.
D13 (= D91) active site, Proton donor; binding Mg(2+)
E20 (= E98) binding substrate
R56 (= R134) binding substrate
SG 99:100 (= SG 179:180) binding substrate
K144 (= K223) binding substrate
D167 (= D246) binding Mg(2+)
N170 (= N249) binding substrate

1l7nA Transition state analogue of phosphoserine phosphatase (aluminum fluoride complex) (see paper)
41% identity, 65% coverage: 81:289/324 of query aligns to 1:209/209 of 1l7nA

query
sites
1l7nA

R

K

K

K

L

L

L

I

L

L

A

F

D
|
D

M
x
F

D
|
D

S

S

T

T

V

L

I

V

G

N

Q

N

E

E

C

T

I

I

N

D

E

E

L

I

A

A

Q

R

E

E

M

A

G

G

K

V

R

E

A

E

Q

E

V

V

T

K

K

K

I

I

T

T

E

K

Q

E

A

A

M

M

A

E

G

G

E

K

I

L

D

N

F

F

E

E

T

Q

S

S

L

L

R

R

E

K

R

R

V

V

T

S

V

L

I

L

K

K

N

D

L

L

R

P

A

I

D

E

T

K

V

V

N

E

R

K

I

A

L

I

G

-

K

K

K

R

I

I

T

T

I

P

T

T

N

E

G

G

A

A

R

E

V

E

L

T

V

I

Q

K

T

E

M

L

R

K

A

-

N

N

R

R

A

G

Y

Y

T

V

V

V

L

A

V

V

T

V

S
|
S

G
|
G

-

G

F

F

S

D

A

I

F

A

A

V

A

N

P

K

V

I

T

K

E

E

K

K

V

L

G

G

F

L

N

D

E

Y

F

A

Q

F

A

A

N

N

V

R

L

L

G

I

V

V

A

K

E

D

N

G

R

K

F

L

T

T

G

G

L

D

L

V

E

E

E

G

P

E

V

V

L

L

G

K

T

E

E

N

A

A

K
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K

R

G

D

E

I

I

L

L

I

E

R

K

L

I

R

A

E

K

K

I

E

E

F

G

L

I

P

N

K

L

V

E

Q

D

V

T

M

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N
|
N

D
|
D

L

I

A

S

M

M

L

F

A

K

E

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

H

C

A

A

K

K

P

P

A

I

V

L

A

K

A

E

A

K

A

A

D

D

A

I

R

C

I

I

D

E

H

K

A

R

D

D

L

L

T

R

A

E

L

I

L

L

-

K

Y

Y

L

I

Q

K

active site: D9 (= D89), F10 (≠ M90), D11 (= D91), G98 (= G180), K142 (= K223), D169 (= D250)
binding aluminum fluoride: D9 (= D89), F10 (≠ M90), D11 (= D91), S97 (= S179), K142 (= K223)
binding tetrafluoroaluminate ion: D9 (= D89), F10 (≠ M90), D11 (= D91), S97 (= S179), G98 (= G180), K142 (= K223), N168 (= N249)
binding magnesium ion: D9 (= D89), D11 (= D91), D165 (= D246)

1l7pA Substrate bound phosphoserine phosphatase complex structure (see paper)
40% identity, 64% coverage: 82:289/324 of query aligns to 1:208/208 of 1l7pA

query
sites
1l7pA

R

K

K

K

L

L

L

I

L

L

A

F

D
x
N

M
x
F

D
|
D

S

S

T

T

V

L

I

V

G

N

Q

N

E
|
E

C

T

I

I

N

D

E

E

L

I

A

A

Q

R

E

E

M

A

G

G

K

V

R

E

A

E

Q

E

V

V

T

K

K

K

I

I

T

T

E

K

Q

E

A

A

M
|
M

A

E

G

G

E

K

I

L

D

N

F
|
F

E

E

T

Q

S

S

L

L

R

R

E

K

R
|
R

V

V

T

S

V

L

I

L

K

K

N

D

L

L

R

P

A

I

D

E

T

K

V

V

N

E

R

K

I

A

L

I

G

-

K

K

K

R

I

I

T

T

I

P

T

T

N

E

G

G

A

A

R

E

V

E

L

T

V

I

Q

K

T

E

M

L

R

K

A

-

N

N

R

R

A

G

Y

Y

T

V

V

V

L

A

V

V

T

V

S
|
S

G
|
G

-

G

F

F

S

D

A

I

F

A

A

V

A

N

P

K

V

I

T

K

E

E

K

K

V

L

G

G

F

L

N

D

E

Y

F

A

Q

F

A

A

N

N

V

R

L

L

G

I

V

V

A

K

E

D

N

G

R

K

F

L

T

T

G

G

L

D

L

V

E

E

E

G

P

E

V

V

L

L

G

K

T

E

E

N

A

A

K
|
K

R

G

D

E

I

I

L

L

I

E

R

K

L

I

R

A

E

K

K

I

E

E

F

G

L

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P

N

K

L

V

E

Q

D

V

T

M

V

A

A

V

V

G

G

D

D

G

G

A

A

N

N

D
|
D

L

I

A

S

M

M

L

F

A

K

E

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

H

C

A

A

K

K

P

P

A

I

V

L

A

K

A

E

A

K

A

A

D

D

A

I

R

C

I

I

D

E

H

K

A

R

D

D

L

L

T

R

A

E

L

I

L

L

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K

Y

Y

L

I

Q

K

active site: N8 (≠ D89), F9 (≠ M90), D10 (= D91), G97 (= G180), K141 (= K223), D168 (= D250)
binding phosphoserine: N8 (≠ D89), F9 (≠ M90), D10 (= D91), E17 (= E98), M40 (= M121), F46 (= F127), R53 (= R134), S96 (= S179), G97 (= G180), K141 (= K223)

8a21A Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with phenylimidazole (see paper)
34% identity, 79% coverage: 45:300/324 of query aligns to 140:393/396 of 8a21A

query
sites
8a21A

I

V

T

S

F

V

T

P

P

P

Q

G

A

A

G

D

T

E

D

A

L

L

R

R

A

T

L

A

A

L

D

N

Q

R

I

V

R

S

A

S

G

E

L

E

H

H

P

-

W

-

Q

-

I

V

D

D

V

V

V

A

L

V

Q

E

-

D

-

Y

P

T

V

L

E

E

G

R

R

R

R

A

K

K

K

R

L

L

L

I

L

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A

F

D
|
D

M

V

D
|
D

S

S

T

T

V

L

I

V

G

Q

Q

G

E
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E

C
x
V

I
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I

N

E

E

M

L

L

A

A

Q

A

E

K

M

A

G

G

K

A

R

E

A

G

Q

Q

V

V

T

A

K

A

I

I

T
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T

E

D

Q

A

A

A

M
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M

A

R

G

G

E

E

I

L

D

D

F
|
F

E

A

T

Q

S

S

L

L

R

Q

E

Q

R
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R

V

V

T

A

V

T

I

L

K

A

N

G

L

L

R

P

A

A

D

T

T

V

V

I

N

D

R

E

I

V

L

A

G

G

K

Q

K

-

I

L

T

E

I

L

T

M

N

P

G

G

A

A

R

R

V

T

L

T

V

L

Q

R

T

T

M

L

R

R

A

R

N

L

R

G

A

Y

Y

A

T

C

V

G

L

V

V

V

T

S

S

G

G
|
G

F

F

S

R

A

R

F

I

A

I

A

E

P

P

V

L

T

A

E

E

K

E

V

L

G

M

F

L

N

D

E

Y

F

V

Q

A

A

A

N

N

V

E

L

L

G

E

V

I

A

V

E

D

N

G

R

T

F

L

T

T

G

G

L

R

L

V

E

V

E

G

P

P

V

I

L

I

G

D

T

R

E

A

A

G

K

K

R

A

D

T

I

A

L

L

I

R

R

E

L

F

R

A

E

Q

K

R

E

A

F

G

L

V

P

P

K

M

V

A

Q

Q

V

T

M

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

A

D

M

M

L

L

A

A

E

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

A

A

V

L

A

R

A

E

A

V

A

A

D

D

A

A

R

S

I

L

D

S

H

H

A

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

Q

T

R

R

S

G

Q

E

F

I

W

E

S

A

E

A

E

D

binding magnesium ion: D183 (= D89), D185 (= D91), D339 (= D246)
binding 4-phenyl-1h-imidazole: D185 (= D91), E192 (= E98), V193 (≠ C99), I194 (= I100), T211 (= T117), M215 (= M121), F221 (= F127), R228 (= R134), G273 (= G180)

Sites not aligning to the query:

binding 4-phenyl-1h-imidazole: 13, 18, 37

8a1zA Crystal structure of phosphoserine phosphatase serb from mycobacterium avium in complex with 1-(2,4-dichlorophenyl)-3-hydroxyurea (see paper)
34% identity, 79% coverage: 45:300/324 of query aligns to 140:393/396 of 8a1zA

query
sites
8a1zA

I

V

T

S

F

V

T

P

P

P

Q

G

A

A

G

D

T

E

D

A

L

L

R

R

A

T

L

A

A

L

D

N

Q

R

I

V

R

S

A

S

G

E

L

E

H

H

P

-

W

-

Q

-

I

V

D

D

V

V

V

A

L

V

Q

E

-

D

-

Y

P

T

V

L

E

E

G

R

R

R

R

A

K

K

K

R

L

L

L

I

L

V

A

F

D
|
D

M

V

D
|
D

S

S

T

T

V

L

I

V

G

Q

Q

G

E
|
E

C

V

I

I

N

E

E

M

L

L

A

A

Q

A

E

K

M

A

G

G

K

A

R

E

A

G

Q

Q

V

V

T

A

K

A

I

I

T

T

E

D

Q

A

A

A

M
|
M

A

R

G

G

E

E

I

L

D

D

F
|
F

E

A

T

Q

S

S

L
|
L

R

Q

E

Q

R
|
R

V

V

T

A

V

T

I

L

K

A

N

G

L

L

R

P

A

A

D

T

T

V

V

I

N

D

R

E

I

V

L

A

G

G

K

Q

K

-

I

L

T

E

I

L

T

M

N

P

G

G

A

A

R

R

V

T

L

T

V

L

Q

R

T

T

M

L

R

R

A

R

N

L

R

G

A

Y

Y

A

T

C

V

G

L

V

V

V

T

S

S
x
G

G

G

F
|
F

S

R

A

R

F

I

A

I

A

E

P

P

V

L

T

A

E

E

K

E

V

L

G

M

F

L

N

D

E

Y

F

V

Q

A

A

A

N

N

V

E

L

L

G

E

V

I

A

V

E

D

N

G

R

T

F

L

T

T

G

G

L

R

L

V

E

V

E

G

P

P

V

I

L

I

G

D

T

R

E

A

A

G

K

K

R

A

D

T

I

A

L

L

I

R

R

E

L

F

R

A

E

Q

K

R

E

A

F

G

L

V

P

P

K

M

V

A

Q

Q

V

T

M

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

A

D

M

M

L

L

A

A

E

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

A

A

V

L

A

R

A

E

A

V

A

A

D

D

A

A

R

S

I

L

D

S

H

H

A

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

Q

T

R

R

S

G

Q

E

F

I

W

E

S

A

E

A

E

D

binding 1-(2,4-dichlorophenyl)-3-oxidanyl-urea: D185 (= D91), E192 (= E98), M215 (= M121), F221 (= F127), L225 (= L131), R228 (= R134), G272 (≠ S179), F274 (= F181), D339 (= D246)
binding magnesium ion: D183 (= D89), D185 (= D91), D339 (= D246)

5jlpA Crystal structure of mycobacterium avium serb2 in complex with serine at act domain
34% identity, 79% coverage: 45:300/324 of query aligns to 140:393/396 of 5jlpA

query
sites
5jlpA

I

V

T

S

F

V

T

P

P

P

Q

G

A

A

G

D

T

G

D

A

L

L

R

R

A

T

L

A

A

L

D

N

Q

R

I

V

R

S

A

S

G

E

L

E

H

H

P

-

W

-

Q

-

I

V

D

D

V

V

V

A

L

V

Q

E

-

D

-

Y

P

T

V

L

E

E

G

R

R

R

R

A

K

K

K

R

L

L

L

I

L

V

A

F

D
|
D

M
x
V

D
|
D

S

S

T

T

V

L

I

V

G

Q

Q

G

E

E

C

V

I

I

N

E

E

M

L

L

A

A

Q

A

E

K

M

A

G

G

K

A

R

E

A

G

Q

Q

V

V

T

A

K

A

I

I

T

T

E

D

Q

A

A

A

M

M

A

R

G

G

E

E

I

L

D

D

F

F

E

A

T

Q

S

S

L

L

R

Q

E

Q

R

R

V

V

T

A

V

T

I

L

K

A

N

G

L

L

R

P

A

A

D

T

T

V

V

I

N

D

R

E

I

V

L

A

G

G

K

Q

K

-

I

L

T

E

I

L

T

M

N

P

G

G

A

A

R

R

V

T

L

T

V

L

Q

R

T

T

M

L

R

R

A

R

N

L

R

G

A

Y

Y

A

T

C

V

G

L

V

V

V

T

S

S
x
G

G

G

F

F

S

R

A

R

F

I

A

I

A

E

P

P

V

L

T

A

E

E

K

E

V

L

G

M

F

L

N

D

E

Y

F

V

Q

A

A

A

N

N

V

E

L

L

G

E

V

I

A

V

E

D

N

G

R

T

F

L

T

T

G

G

L

R

L

V

E

V

E

G

P

P

V

I

L

I

G

D

T

R

E

A

A

G

K
|
K

R

A

D

T

I

A

L

L

I

R

R

E

L

F

R

A

E

Q

K

R

E

A

F

G

L

V

P

P

K

M

V

A

Q

Q

V

T

M

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

A

D

M

M

L

L

A

A

E

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

A

A

V

L

A

R

A

E

A

V

A

A

D

D

A

A

R

S

I

L

D

S

H

H

A

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

Q

T

R

R

S

G

Q

E

F

I

W

E

S

A

E

A

E

D

active site: D183 (= D89), V184 (≠ M90), D185 (= D91), G272 (≠ S179), K316 (= K223), D343 (= D250)
binding magnesium ion: D183 (= D89), D339 (= D246)

Sites not aligning to the query:

binding serine: 13, 14

A0QJI1 Phosphoserine phosphatase; PSP; PSPase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Mycobacterium avium (strain 104) (see paper)
34% identity, 79% coverage: 45:300/324 of query aligns to 144:397/411 of A0QJI1

query
sites
A0QJI1

I

V

T

S

F

V

T

P

P

P

Q

G

A

A

G

D

T

G

D

A

L

L

R

R

A

T

L

A

A

L

D

N

Q

R

I

V

R

S

A

S

G

E

L

E

H

H

P

-

W

-

Q

-

I

V

D

D

V

V

V

A

L

V

Q

E

-

D

-

Y

P

T

V

L

E

E

G

R

R

R

R

A

K

K

K

R

L

L

L

I

L

V

A

F

D
|
D

M

V

D
|
D

S

S

T

T

V

L

I

V

G

Q

Q

G

E

E

C

V

I

I

N

E

E

M

L

L

A

A

Q

A

E

K

M

A

G

G

K

A

R

E

A

G

Q

Q

V

V

T

A

K

A

I

I

T

T

E

D

Q

A

A

A

M

M

A

R

G

G

E

E

I

L

D

D

F

F

E

A

T

Q

S

S

L

L

R

Q

E

Q

R

R

V

V

T

A

V

T

I

L

K

A

N

G

L

L

R

P

A

A

D

T

T

V

V

I

N

D

R

E

I

V

L

A

G

G

K

Q

K

-

I

L

T

E

I

L

T

M

N

P

G

G

A

A

R

R

V

T

L

T

V

L

Q

R

T

T

M

L

R

R

A

R

N

L

R

G

A

Y

Y

A

T

C

V

G

L

V

V

V

T

S

S

G

G

G

F

F

S

R

A

R

F

I

A

I

A

E

P

P

V

L

T

A

E

E

K

E

V

L

G

M

F

L

N

D

E

Y

F

V

Q

A

A

A

N

N

V

E

L

L

G

E

V

I

A

V

E

D

N

G

R

T

F

L

T

T

G

G

L

R

L

V

E

V

E

G

P

P

V

I

L

I

G

D

T

R

E

A

A

G

K

K

R

A

D

T

I

A

L

L

I

R

R

E

L

F

R

A

E

Q

K

R

E

A

F

G

L

V

P

P

K

M

V

A

Q

Q

V

T

M

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D

D

L

I

A

D

M

M

L

L

A

A

E

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

A

A

V

L

A

R

A

E

A

V

A

A

D

D

A

A

R

S

I

L

D

S

H

H

A

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

Q

T

R

R

S

G

Q

E

F

I

W

E

S

A

E

A

E

D

D187 (= D89) binding Mg(2+)
D189 (= D91) binding Mg(2+)
D343 (= D246) binding Mg(2+)

O53289 Phosphoserine phosphatase SerB2; PSP; PSPase; O-phosphoserine phosphohydrolase; Protein-serine/threonine phosphatase; EC 3.1.3.3; EC 3.1.3.16 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
35% identity, 68% coverage: 81:300/324 of query aligns to 177:395/409 of O53289

query
sites
O53289

R

R

R

T

K

K

K

R

L

L

L

I

L

V

A

F

D
|
D

M
x
V

D
|
D

S
|
S

T

T

V

L

I

V

G

Q

Q

G

E

E

C

V

I

I

N

E

E

M

L

L

A

A

Q

A

E

R

M

A

G

G

K

A

R

Q

A

G

Q

Q

V

V

T

A

K

A

I

I

T

T

E

E

Q

A

A

A

M

M

A

R

G

G

E

E

I

L

D

D

F

F

E

A

T

E

S

S

L

L

R

Q

E

R

R

R

V

V

T

A

V

T

I

L

K

A

N

G

L

L

R

P

A

A

D

T

T

V

V

I

N

D

R

D

I

V

L

-

G

A

K

E

K

Q

I

L

T

E

I

L

T

M

N

P

G

G

A

A

R

R

V

T

L

T

V

I

Q

R

T

T

M

L

R

R

A

R

N

L

R

G

A

F

Y

R

T

C

V

G

L

V

V

V

T
x
S

S

G

G

G

F

F

S

R

A

R

F

I

A

I

A

E

P

P

V

L

T

A

E

R

K

E

V

L

G

M

F

L

N

D

E

F

F

V

Q

A

A

S

N

N

V

E

L

L

G

E

V

I

A

V

E

D

N

G

R

I

F

L

T

T

G

G

L

R

L

V

E

V

E

G

P

P

V

I

L

V

G

D

T

R

E

P

A

G

K
|
K

R

A

D

K

I

A

L

L

I

R

R

D

L

F

R

A

E

S

K

Q

E

Y

F

G

L

V

P

P

K

M

V

E

Q

Q

V

T

M

V

A

A

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

I

A

D

M

M

L

L

A

G

E

A

A

A

G

G

L

L

G

G

V

I

A

A

F

F

H

N

A

A

K

K

P

P

A

A

V

L

A

R

A

E

A

V

A

A

D

D

A

A

R

S

I

L

D

S

H

H

A

P

D

Y

L

L

T

D

A

T

L

V

L

L

Y

F

L

L

Q

L

G

G

Y

V

Q

T

R

R

S

G

Q

E

F

I

W

E

S

A

E

A

E

D

D185 (= D89) mutation to G: Completely abolishes enzymatic activity.; mutation to N: Completely abolishes enzymatic activity.
V186 (≠ M90) mutation to Q: Decreases enzymatic activity by 50%.
D187 (= D91) mutation to N: Decreases enzymatic activity by 15%.
S188 (= S92) mutation to A: No effect on enzymatic activity.
S273 (≠ T178) mutation to A: Completely abolishes enzymatic activity (PubMed:25521849). Decreases enzymatic activity by 60% (PubMed:25037224).
K318 (= K223) mutation to A: Decreases enzymatic activity by 50%.; mutation to E: Completely abolishes enzymatic activity.
D341 (= D246) mutation to G: Decreases enzymatic activity by 80%.; mutation to N: Decreases enzymatic activity by 85%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-345.
D345 (= D250) mutation to N: Decreases enzymatic activity by 55%. Completely abolishes enzymatic activity, does not elicit cytoskeletal rearrangements, and does not suppress IL-8 production after TNF-alpha stimulation; when associated with N-341.

Sites not aligning to the query:

18 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.
108 G→A: Does not bind L-serine and correspondingly no oligomeric transitions is observed in the presence of L-serine.

1l7oA Crystal structure of phosphoserine phosphatase in apo form (see paper)
39% identity, 64% coverage: 82:289/324 of query aligns to 1:200/200 of 1l7oA

query
sites
1l7oA

R

K

K

K

L

L

L

I

L

L

A

F

D

N

M

F

D

D

S

S

T

T

V

L

I

V

G

N

Q

N

E

E

C

T

I

I

N

D

E

E

L

I

A

A

Q

R

E

E

M

A

G

G

K

V

R

E

A

E

Q

E

V

V

T

K

K

K

I

I

T

T

E

-

Q

-

A

-

M

-

A

-

G

-

E

-

I

-

D

N

F

F

E

E

T

Q

S

S

L

L

R

R

E

K

R

R

V

V

T

S

V

L

I

L

K

K

N

D

L

L

R

P

A

I

D

E

T

K

V

V

N

E

R

K

I

A

L

I

G

-

K

K

K

R

I

I

T

T

I

P

T

T

N

E

G

G

A

A

R

E

V

E

L

T

V

I

Q

K

T

E

M

L

R

K

A

-

N

N

R

R

A

G

Y

Y

T

V

V

V

L

A

V

V

T

V

S

S

G

G

-

G

F

F

S

D

A

I

F

A

A

V

A

N

P

K

V

I

T

K

E

E

K

K

V

L

G

G

F

L

N

D

E

Y

F

A

Q

F

A

A

N

N

V

R

L

L

G

I

V

V

A

K

E

D

N

G

R

K

F

L

T

T

G

G

L

D

L

V

E

E

E

G

P

E

V

V

L

L

G

K

T

E

E

N

A

A

K

K

R

G

D

E

I

I

L

L

I

E

R

K

L

I

R

A

E

K

K

I

E

E

F

G

L

I

P

N

K

L

V

E

Q

D

V

T

M

V

A

A

V

V

G

G

D

D

G

G

A

A

N

N

D

D

L

I

A

S

M

M

L

F

A

K

E

K

A

A

G

G

L

L

G

K

V

I

A

A

F

F

H

C

A

A

K

K

P

P

A

I

V

L

A

K

A

E

A

K

A

A

D

D

A

I

R
x
C

I

I

D
x
E

H

K

A

R

D

D

L

L

T

R

A

E

L

I

L

L

-

K

Y

Y

L

I

Q

K

binding zinc ion: C186 (≠ R276), E188 (≠ D278)

3m1yC Crystal structure of a phosphoserine phosphatase (serb) from helicobacter pylori
30% identity, 62% coverage: 83:282/324 of query aligns to 3:200/208 of 3m1yC

query
sites
3m1yC

K

Q

K

K

L

L

L

A

L

V

A

F

D
|
D

M
x
F

D
|
D

S

S

T

T

V

L

I

V

G

N

Q

A

E

E

C

T

I

I

N

E

E

S

L

L

A

A

Q

R

E

A

M

W

G

G

K

V

R

F

A

D

Q

E

V

V

T

K

K

T

I

I

T

T

E

L

Q

K

A

A

M

M

A

N

G

G

E

T

I

-

D

D

F

F

E

H

T

K

S

S

L

L

R

I

E

L

R

R

V

V

T

S

V

K

I

L

K

K

N

N

L

M

R

P

A

L

D

K

T

L

V

A

N

K

R

E

I

V

L

C

G

-

K

E

K

S

I

L

T

P

I

L

T

F

N

E

G

G

A

A

R

L

V

E

L

L

V

V

Q

S

T

A

M

L

R

K

A

E

N

K

R

N

A

Y

Y

K

T

V

V

V

L

C

V

F

T

S

S
x
G

G

G

F

F

S

D

A

L

F

A

A

T

A

N

P

H

V

Y

T

R

E

D

K

L

V

L

G

H

F

L

N

D

E

A

F

A

Q

F

A

S

N

N

V

T

L

L

G

I

V

V

A

E

E

N

N

D

R

A

F

L

T

N

G

G

L

L

L

V

E

T

E

G

P

H

V

M

L

M

G

F

T

S

E

H

A

S

K
|
K

R

G

D

E

I

M

L

L

I

L

R

V

L

L

R

Q

E

R

K

L

E

L

F

N

L

I

P

S

K

K

V

T

Q

N

V

T

M

L

A

V

V

V

G

G

D
|
D

G

G

A

A

N

N

D
|
D

L

L

A

S

M

M

L

F

A

K

E

H

A

A

G

H

L

I

G

K

V

I

A

A

F

F

H

N

A

A

K

K

P

E

A

V

V

L

A

K

A

Q

A

H

A

A

D

T

A

H

R

C

I

I

D

N

H

E

A

P

D

D

L

L

active site: D9 (= D89), F10 (≠ M90), D11 (= D91), G97 (≠ S179), K141 (= K223), D168 (= D250)
binding magnesium ion: D9 (= D89), D11 (= D91), D164 (= D246)

4ap9A Crystal structure of phosphoserine phosphatase from t.Onnurineus in complex with ndsb-201 (see paper)
30% identity, 56% coverage: 83:262/324 of query aligns to 9:174/200 of 4ap9A

query
sites
4ap9A

K

K

L

V

L

A

L

V

A

I

D
|
D

M
x
I

D
x
E

S

G

T

T

V

L

I

T

G

D

Q

F

E

E

C

F

I

W

N

R

E

E

L

M

A

A

Q
x
R

E
x
I

M
x
T

G

G

K

K

R

R

A

-

Q

E

V

I

T

E

K

E

I

L

T

L

E

E

Q

K

A

G

M
x
L

A

S

G

G

E

E

I

V

D

E

F
x
W

E

L

T

D

S

S

L

L

R

L

E

K

R

R

V

V

T

G

V

L

I

I

K

R

N

G

L

I

R

D

A

E

D

G

T

T

V

F

N

L

R

R

I

T

L

R

G

-

K

E

K

K

I

V

T

N

I

V

T

S

N

P

G

E

A

A

R

R

V

E

L

L

V

V

Q

E

T

T

M

L

R

R

A

E

N

K

R

G

A

F

Y

K

T

V

V

V

L

L

V

I

T

S

S
x
G

G

S

F

F

S

E

A

E

F

V

A

L

A

E

P

P

V

F

T

K

E

E

K

-

V

L

G

G

F

-

N

D

E

E

F

F

Q

M

A

A

N

N

V

R

L

A

G

I

V

F

A

E

E

D

N

G

R

K

F

F

T

Q

G

G

L

-

E

-

P

-

V

-

L

-

G

-

T

-

E

-

A

-

K

-

R

-

D

-

I

I

L

R

I

L

R

R

L

F

R

R

E
x
D

K
|
K

-

G

E

E

F

F

L

L

P

K

K

R

V

F

Q

R

-

D

-

G

-

F

V

I

M

L

A

A

V

M

G

G

D

D

G

G

A
x
Y

N
x
A

D
|
D

L

A

A

K

M

M

L

F

A

E

E

R

A

A

G

D

L

M

G

G

V

I

A

A

active site: D15 (= D89), I16 (≠ M90), E17 (≠ D91), G103 (≠ S179), K141 (= K233), D162 (= D250)
binding 3-pyridinium-1-ylpropane-1-sulfonate: R31 (≠ Q105), I32 (≠ E106), T33 (≠ M107), L46 (≠ M121), W52 (≠ F127), D140 (≠ E232), K141 (= K233), Y160 (≠ A248), A161 (≠ N249)

6q6jB Human phosphoserine phosphatase with substrate analogue homo-cysteic acid (see paper)
28% identity, 54% coverage: 89:263/324 of query aligns to 16:192/217 of 6q6jB

query
sites
6q6jB

D
|
D

M
x
V

D
|
D

S

S

T

T

V

V

I

I

G

R

Q

E

E

E

C

G

I

I

N

D

E

E

L

L

A

A

Q

K

E

I

M

C

G

G

K

V

R

E

A

D

Q

A

V

V

T

S

K

E

I

M

T

T

E

R

Q

R

A

A

M

M

A

G

G

G

E

A

I

V

D

P

F
|
F

E

K

T

A

S

A

L

L

R

T

E

E

R

R

V

L

T

A

V

L

I

I

K

Q

N

P

L

S

R

R

A

E

D

Q

T

V

V

Q

N

R

R

L

I

I

L

A

G

E

K

Q

K

P

I

P

T

H

I

L

T

T

N

P

G

G

A

I

R

R

V

E

L

L

V

V

Q

S

T

R

M

L

R

Q

A

E

N

R

R

N

A

V

Y

Q

T

V

V

F

L

L

V

I

T
x
S

S
x
G

G
|
G

F

F

S

R

A

S

F

I

A

V

A

E

P

H

V

V

T

A

E

S

K

K

V

L

G

N

F

I

-

P

-

A

-

T

N

N

E

V

F

F

Q

A

A

N

N

R

V

L

L

K

G

F

V

Y

A

F

E

N

N

G

R

E

F

Y

T

A

G

G

L

F

L

D

E

E

-

T

E

Q

P

P

V

T

L

A

G

E

T

S

E

G

A

G

K
|
K

R

G

D

K

I

V

L

I

I

K

R

L

L

L

R

K

E

E

K

K

E

F

F

H

L

F

P

K

K

K

V

-

Q

-

V

I

M

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

A

E

M

A

L

C

A

P

E

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

binding calcium ion: D16 (= D89), D18 (= D91), D175 (= D246)
binding (2~{S})-2-azanyl-4-sulfo-butanoic acid: D16 (= D89), V17 (≠ M90), D18 (= D91), F54 (= F127), S105 (≠ T178), G106 (≠ S179), G107 (= G180), K154 (= K223), T178 (≠ N249)

6hyjB Psph human phosphoserine phosphatase (see paper)
28% identity, 54% coverage: 89:263/324 of query aligns to 20:196/223 of 6hyjB

query
sites
6hyjB

D
|
D

M

V

D
|
D

S

S

T

T

V

V

I

I

G

R

Q

E

E
|
E

C

G

I

I

N

D

E

E

L

L

A

A

Q

K

E

I

M

C

G

G

K

V

R

E

A

D

Q

A

V

V

T

S

K

E

I

M

T

T

E

R

Q

R

A

A

M

M

A

G

G

G

E

A

I

V

D

P

F

F

E

K

T

A

S

A

L

L

R

T

E

E

R

R

V

L

T

A

V

L

I

I

K

Q

N

P

L

S

R

R

A

E

D

Q

T

V

V

Q

N

R

R

L

I

I

L

A

G

E

K

Q

K

P

I

P

T

H

I

L

T

T

N

P

G

G

A

I

R

R

V

E

L

L

V

V

Q

S

T

R

M

L

R

Q

A

E

N

R

R

N

A

V

Y

Q

T

V

V

F

L

L

V

I

T

S

S

G

G

G

F

F

S

R

A

S

F

I

A

V

A

E

P

H

V

V

T

A

E

S

K

K

V

L

G

N

F

I

-

P

-

A

-

T

N

N

E

V

F
|
F

Q

A

A

N

N

R

V

L

L

K

G

F

V

Y

A

F

E

N

N

G

R

E

F

Y

T

A

G

G

L

F

L

D

E

E

-

T

E

Q

P

P

V

T

L

A

G

E

T

S

E

G

A

G

K

K

R

G

D

K

I

V

L

I

I

K

R

L

L

L

R

K

E

E

K
|
K

E

F

F

H

L

F

P

K

K

K

V

-

Q

-

V

I

M

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

A

E

M

A

L

C

A

P

E

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

binding calcium ion: D20 (= D89), D22 (= D91), D179 (= D246)
binding phosphoserine: F131 (= F197), K168 (= K233)
binding serine: E29 (= E98)

Sites not aligning to the query:

binding serine: 202

1l8oA Molecular basis for the local conformational rearrangement of human phosphoserine phosphatase (see paper)
28% identity, 54% coverage: 89:263/324 of query aligns to 17:193/222 of 1l8oA

query
sites
1l8oA

D
|
D

M
x
V

D
|
D

S

S

T

T

V

V

I

I

G

R

Q

E

E

E

C

G

I

I

N

D

E

E

L

L

A

A

Q

K

E

I

M

C

G

G

K

V

R

E

A

D

Q

A

V

V

T

S

K

E

I

M

T

T

E

R

Q

R

A

A

M

M

A

G

G

G

E

A

I

V

D

P

F

F

E

K

T

A

S

A

L

L

R

T

E

E

R

R

V

L

T

A

V

L

I

I

K

Q

N

P

L

S

R

R

A

E

D

Q

T

V

V

Q

N

R

R

L

I

I

L

A

G

E

K

Q

K

P

I

P

T

H

I

L

T

T

N

P

G

G

A

I

R

R

V

E

L

L

V

V

Q

S

T

R

M

L

R

Q

A

E

N

R

R

N

A

V

Y

Q

T

V

V

F

L

L

V

I

T
x
S

S
x
G

G

G

F

F

S

R

A

S

F

I

A

V

A

E

P

H

V

V

T

A

E

S

K

K

V

L

G

N

F

I

-

P

-

A

-

T

N

N

E

V

F

F

Q

A

A

N

N

R

V

L

L

K

G

F

V

Y

A

F

E

N

N

G

R

E

F

Y

T

A

G

G

L

F

L

D

E

E

-

T

E

Q

P

P

V

T

L

A

G

E

T

S

E

G

A

G

K
|
K

R

G

D

K

I

V

L

I

I

K

R

F

L

L

R

K

E

E

K

K

E

F

F

H

L

F

P

K

K

K

V

-

Q

-

V

I

M

I

A

M

V

I

G

G

D

D

G
|
G

A

A

N
x
T

D
|
D

L

M

A

E

M

A

L

C

A

P

E

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

active site: D17 (= D89), V18 (≠ M90), D19 (= D91), G107 (≠ S179), K155 (= K223), D180 (= D250)
binding phosphate ion: D17 (= D89), D19 (= D91), S106 (≠ T178), K155 (= K223)
binding serine: G177 (= G247), T179 (≠ N249)

Sites not aligning to the query:

binding serine: 199

1l8lA Molecular basis for the local confomational rearrangement of human phosphoserine phosphatase (see paper)
28% identity, 54% coverage: 89:263/324 of query aligns to 17:193/222 of 1l8lA

query
sites
1l8lA

D
|
D

M
x
V

D
|
D

S

S

T

T

V

V

I

I

G

R

Q

E

E

E

C

G

I

I

N

D

E

E

L

L

A

A

Q

K

E

I

M

C

G

G

K

V

R

E

A

D

Q

A

V

V

T

S

K

E

I

M

T

T

E

R

Q

R

A

A

M

M

A

G

G

G

E

A

I

V

D

P

F

F

E

K

T

A

S

A

L

L

R

T

E

E

R

R

V

L

T

A

V

L

I

I

K

Q

N

P

L

S

R

R

A

E

D

Q

T

V

V

Q

N

R

R

L

I

I

L

A

G

E

K

Q

K

P

I

P

T

H

I

L

T

T

N

P

G

G

A

I

R

R

V

E

L

L

V

V

Q

S

T

R

M

L

R

Q

A

E

N

R

R

N

A

V

Y

Q

T

V

V

F

L

L

V

I

T

S

S
x
G

G

G

F

F

S

R

A

S

F

I

A

V

A

E

P

H

V

V

T

A

E

S

K

K

V

L

G

N

F

I

-

P

-

A

-

T

N

N

E

V

F

F

Q

A

A

N

N

R

V

L

L

K

G

F

V

Y

A

F

E

N

N

G

R

E

F

Y

T

A

G

G

L

F

L

D

E

E

-

T

E

Q

P

P

V

T

L

A

G

E

T

S

E

G

A

G

K
|
K

R

G

D

K

I

V

L

I

I

K

R

F

L

L

R

K

E

E

K

K

E

F

F

H

L

F

P

K

K

K

V

-

Q

-

V

I

M

I

A

M

V

I

G

G

D
|
D

G
|
G

A

A

N
x
T

D
|
D

L

M

A

E

M

A

L

C

A

P

E

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

active site: D17 (= D89), V18 (≠ M90), D19 (= D91), G107 (≠ S179), K155 (= K223), D180 (= D250)
binding d-2-amino-3-phosphono-propionic acid: D17 (= D89), D19 (= D91), G107 (≠ S179), K155 (= K223), D176 (= D246), G177 (= G247), T179 (≠ N249)

P78330 Phosphoserine phosphatase; PSP; PSPase; L-3-phosphoserine phosphatase; O-phosphoserine phosphohydrolase; EC 3.1.3.3 from Homo sapiens (Human) (see 4 papers)
28% identity, 54% coverage: 89:263/324 of query aligns to 20:196/225 of P78330

query
sites
P78330

D
|
D

M
x
V

D
|
D

S
|
S

T

T

V

V

I

I

G

R

Q

E

E
|
E

C

G

I

I

N
x
D

E

E

L

L

A
|
A

Q

K

E

I

M

C

G

G

K

V

R

E

A

D

Q

A

V

V

T

S

K

E

I

M

T

T

E

R

Q

R

A

A

M
|
M

A
x
G

G

G

E

A

I

V

D

P

F

F

E

K

T

A

S

A

L

L

R

T

E

E

R
|
R

V

L

T

A

V

L

I

I

K

Q

N

P

L

S

R

R

A

E

D

Q

T

V

V

Q

N

R

R

L

I

I

L

A

G

E

K

Q

K

P

I

P

T

H

I

L

T

T

N

P

G

G

A

I

R

R

V

E

L

L

V

V

Q

S

T

R

M

L

R

Q

A

E

N

R

R

N

A

V

Y

Q

T

V

V

F

L

L

V

I

T
x
S

S
x
G

G
|
G

F

F

S

R

A

S

F

I

A

V

A

E

P

H

V

V

T

A

E

S

K

K

V

L

G

N

F

I

-

P

-

A

-

T

N

N

E

V

F

F

Q

A

A
x
N

N

R

V

L

L

K

G

F

V

Y

A

F

E

N

N

G

R

E

F

Y

T

A

G

G

L

F

L

D

E

E

-

T

E

Q

P

P

V

T

L

A

G

E

T

S

E

G

A

G

K
|
K

R

G

D

K

I

V

L

I

I

K

R

L

L

L

R

K

E

E

K

K

E

F

F

H

L

F

P

K

K

K

V

-

Q

-

V

I

M

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N
x
T

D

D

L

M

A

E

M

A

L

C

A

P

E

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

D20 (= D89) binding Mg(2+)
DVD 20:22 (≠ DMD 89:91) binding L-serine
D22 (= D91) binding Mg(2+)
S23 (= S92) mutation to A: Reduces L-phosphoserine phosphatase activity by about 50%.; mutation to T: Reduces L-phosphoserine phosphatase activity by about 80%.
E29 (= E98) mutation to D: Reduces L-phosphoserine phosphatase activity by about 95%.; mutation to Q: Loss of L-phosphoserine phosphatase activity.
D32 (≠ N101) to N: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894035
A35 (= A104) to T: in PSPHD; decreased L-phosphoserine phosphatase activity
M52 (= M121) binding O-phospho-L-serine; to T: in PSPHD; decreased L-phosphoserine phosphatase activity; dbSNP:rs104894036
G53 (≠ A122) binding phosphate
R65 (= R134) mutation R->A,K: Loss of L-phosphoserine phosphatase activity.
SGG 109:111 (≠ TSG 178:180) binding L-serine; binding O-phospho-L-serine
N133 (≠ A199) mutation to A: Reduces L-phosphoserine phosphatase activity by about 75%.
K158 (= K223) binding L-serine; binding O-phospho-L-serine
D179 (= D246) binding Mg(2+)
T182 (≠ N249) binding O-phospho-L-serine; binding phosphate; mutation to S: Reduces L-phosphoserine phosphatase activity by about 99%.; mutation to V: Reduces L-phosphoserine phosphatase activity by about 25%.

Sites not aligning to the query:

202 R→A: Reduces L-phosphoserine phosphatase activity by about 99%.; R→K: Reduces L-phosphoserine phosphatase activity by about 95%.

6hyyA Human phosphoserine phosphatase with serine and phosphate (see paper)
28% identity, 54% coverage: 89:263/324 of query aligns to 16:192/221 of 6hyyA

query
sites
6hyyA

D
|
D

M
x
V

D
|
D

S

S

T

T

V

V

I

I

G

R

Q

E

E

E

C

G

I

I

N

D

E

E

L

L

A

A

Q

K

E

I

M

C

G

G

K

V

R

E

A

D

Q

A

V

V

T

S

K

E

I

M

T

T

E

R

Q

R

A

A

M

M

A

G

G

G

E

A

I

V

D

P

F

F

E

K

T

A

S

A

L

L

R

T

E

E

R

R

V

L

T

A

V

L

I

I

K

Q

N

P

L

S

R

R

A

E

D

Q

T

V

V

Q

N

R

R

L

I

I

L

A

G

E

K

Q

K

P

I

P

T

H

I

L

T

T

N

P

G

G

A

I

R

R

V

E

L

L

V

V

Q

S

T

R

M

L

R

Q

A

E

N

R

R

N

A

V

Y

Q

T

V

V

F

L

L

V

I

T
x
S

S
x
G

G

G

F

F

S

R

A

S

F

I

A

V

A

E

P

H

V

V

T

A

E

S

K

K

V

L

G

N

F

I

-

P

-

A

-

T

N

N

E

V

F

F

Q

A

A

N

N

R

V

L

L

K

G

F

V

Y

A

F

E

N

N

G

R

E

F

Y

T

A

G

G

L

F

L

D

E

E

-

T

E

Q

P

P

V

T

L

A

G

E

T

S

E

G

A

G

K
|
K

R

G

D

K

I

V

L

I

I

K

R

L

L

L

R

K

E

E

K

K

E

F

F

H

L

F

P

K

K

K

V

-

Q

-

V

I

M

I

A

M

V

I

G

G

D
|
D

G

G

A

A

N

T

D

D

L

M

A

E

M

A

L

C

A

P

E

P

A

A

G

D

L

A

G

F

V

I

A

G

F

F

binding magnesium ion: D16 (= D89), D18 (= D91), D175 (= D246)
binding serine: D16 (= D89), V17 (≠ M90), D18 (= D91), S105 (≠ T178), G106 (≠ S179), K154 (= K223)

6iuyA Structure of dsgpdh of dunaliella salina (see paper)
26% identity, 57% coverage: 89:274/324 of query aligns to 20:210/585 of 6iuyA

query
sites
6iuyA

D
|
D

M

V

D
|
D

S

R

T

T

V

V

I

T

G

T

Q

D

E

A

C

S

I

V

N

G

E

L

L

L

A

A

Q

K

E

F

M

M

G

G

K

I

R

E

A

D

Q

E

V

A

T

Q

K

S

I

L

T

T

E

E

Q

Q

A

A

M

N

A

R

G

G

E

E

I

I

D

N

F

L

E

T

T

K

S

A

L

F

R

E

E

D

R

R

V

L

T

A

V

K

I

L

K

-

N

N

L

F

R

T

A

P

D

T

T

D

V

I

N

D

R

R

I

F

L

L

G

E

K

E

K

H

I

P

T

A

I

H

T

T

N

R

-

L

-

V

-

P

G

G

A

V

R

E

V

N

L

L

V

I

Q

A

T

A

M

L

R

K

A

A

N

R

R

G

A

V

Y

E

T

V

V

F

L

L

V

I

T

S

S

G

G

G

F

F

S

R

A

E

F

M

A

A

A

L

P

P

V

I

T

A

E

S

K

H

V

L

G

K

F

I

N

P

E

-

F

-

Q

-

A

-

N

-

V

-

L

-

G

-

V

-

A

A

E

K

N

N

R

V

F

F

T

C

G

N

L

T

L

M

E

S

-

W

-

Q

-

L

-

D

-

H

-

G

E

E

P

P

V

V

L

R

G

L

T

S

E

H

A

F

K

K

R

S

D

R

I

A

L

I

I

E

R

R

L

I

R

R

E

R

K

K

E

-

F

-

L

Y

P

P

K

Y

V

N

Q

N

V

I

M

I

A

M

V

V

G

G

D
|
D

G

G

A

F

N

S

D

D

L

L

A

E

M

A

L

M

A

Q

E

G

A

S

G

P

L

D

G

G

-

A

-

D

-

A

-

F

V

I

A

C

F

F

H

G

A

G

-

V

-

M

-

Q

K

R

P

P

A

A

V

V

A

A

A

S

A

Q

A

A

D

D

binding magnesium ion: D20 (= D89), D22 (= D91), D175 (= D246)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 233, 234, 235, 236, 268, 290, 321, 324, 349, 381, 382, 497, 529, 530, 532

Query Sequence

>WP_020175090.1 NCBI__GCF_000385335.1:WP_020175090.1
MTHVATLVCDPSFPIVQASHLELAAGFLPGAQAPQWLDQGVAADITFTPQAGTDLRALAD
QIRAGLHPWQIDVVLQPVEGRRKKLLLADMDSTVIGQECINELAQEMGKRAQVTKITEQA
MAGEIDFETSLRERVTVIKNLRADTVNRILGKKITITNGARVLVQTMRANRAYTVLVTSG
FSAFAAPVTEKVGFNEFQANVLGVAENRFTGLLEEPVLGTEAKRDILIRLREKEFLPKVQ
VMAVGDGANDLAMLAEAGLGVAFHAKPAVAAAADARIDHADLTALLYLQGYQRSQFWSEE
RKELDASEWMKRYGWMARENSRKN

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory