SitesBLAST
Comparing WP_020175690.1 NCBI__GCF_000385335.1:WP_020175690.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
43% identity, 99% coverage: 5:473/473 of query aligns to 3:462/468 of 8i9iA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
43% identity, 99% coverage: 5:473/473 of query aligns to 3:462/471 of P04805
- C98 (= C100) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (≠ A102) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (≠ W127) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (vs. gap) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (vs. gap) mutation to Q: No change in activity or in zinc content.
- H131 (≠ R130) mutation to Q: No change in activity or in zinc content.
- H132 (≠ P131) mutation to Q: No change in activity or in zinc content.
- C138 (≠ P137) mutation to S: No change in activity or in zinc content.
- S239 (= S242) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
42% identity, 98% coverage: 5:469/473 of query aligns to 2:464/468 of 1g59A
- binding : D44 (= D47), R45 (= R48), A46 (≠ E49), R47 (= R50), P109 (= P104), V145 (= V142), R163 (≠ Q160), V166 (= V163), E172 (≠ D169), V177 (= V174), K180 (≠ R177), S181 (= S178), D182 (= D179), E207 (≠ D204), E208 (≠ D205), R237 (≠ H234), K241 (≠ G238), T242 (≠ A239), K243 (= K240), M273 (≠ L270), G274 (= G271), E282 (= E278), S299 (≠ G295), L300 (≠ R296), P303 (≠ A299), V304 (≠ R300), K309 (= K305), W312 (≠ S308), R319 (= R315), P357 (≠ E360), R358 (= R361), R417 (= R422), K426 (= K431), L427 (= L432), Q432 (= Q437), R435 (= R440), L442 (≠ S447), E443 (≠ T448), T444 (≠ S449), P445 (= P450), G446 (= G451), L447 (≠ I452), F448 (= F453)
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
42% identity, 98% coverage: 5:469/473 of query aligns to 2:464/468 of 2cv2A
- active site: K246 (= K243)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R8), A7 (= A10), S9 (= S12), G17 (= G20), I21 (≠ T24), E41 (= E44), Y187 (= Y184), R205 (= R202), A206 (≠ G203), E208 (≠ D205), W209 (≠ H206), L235 (= L232), L236 (≠ I233)
- binding : S9 (= S12), T43 (= T46), D44 (= D47), R47 (= R50), V145 (= V142), R163 (≠ Q160), Y168 (≠ W165), E172 (≠ D169), V177 (= V174), K180 (≠ R177), S181 (= S178), Y187 (= Y184), E207 (≠ D204), E208 (≠ D205), W209 (≠ H206), V211 (≠ T208), R237 (≠ H234), K241 (≠ G238), L272 (≠ R269), M273 (≠ L270), G274 (= G271), E282 (= E278), S299 (≠ G295), P303 (≠ A299), V304 (≠ R300), K309 (= K305), W312 (≠ S308), R319 (= R315), P357 (≠ E360), R358 (= R361), R417 (= R422), Q432 (= Q437), R435 (= R440), L442 (≠ S447), E443 (≠ T448), T444 (≠ S449), G446 (= G451), L447 (≠ I452), F448 (= F453)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
42% identity, 98% coverage: 5:469/473 of query aligns to 2:464/468 of 2cv1A
- active site: K246 (= K243)
- binding adenosine-5'-triphosphate: P8 (= P11), S9 (= S12), G17 (= G20), T18 (≠ G21), I21 (≠ T24), R47 (= R50), A206 (≠ G203), W209 (≠ H206), L235 (= L232), L236 (≠ I233)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R8), A7 (= A10), E41 (= E44), Y187 (= Y184), R205 (= R202), W209 (≠ H206)
- binding : S9 (= S12), E41 (= E44), T43 (= T46), D44 (= D47), R47 (= R50), V145 (= V142), R163 (≠ Q160), V166 (= V163), E172 (≠ D169), V177 (= V174), K180 (≠ R177), S181 (= S178), Y187 (= Y184), E207 (≠ D204), E208 (≠ D205), W209 (≠ H206), V211 (≠ T208), R237 (≠ H234), K241 (≠ G238), K243 (= K240), M273 (≠ L270), G274 (= G271), S276 (= S273), E282 (= E278), S299 (≠ G295), P303 (≠ A299), V304 (≠ R300), K309 (= K305), W312 (≠ S308), R319 (= R315), P357 (≠ E360), R358 (= R361), R417 (= R422), L427 (= L432), Q432 (= Q437), R435 (= R440), L442 (≠ S447), E443 (≠ T448), T444 (≠ S449), G446 (= G451), L447 (≠ I452), F448 (= F453)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
42% identity, 98% coverage: 5:469/473 of query aligns to 2:464/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
42% identity, 98% coverage: 5:469/473 of query aligns to 2:464/468 of 1n78A
- active site: K246 (= K243)
- binding glutamol-amp: R5 (= R8), A7 (= A10), P8 (= P11), S9 (= S12), G17 (= G20), T18 (≠ G21), I21 (≠ T24), E41 (= E44), Y187 (= Y184), N191 (≠ V188), R205 (= R202), A206 (≠ G203), E208 (≠ D205), W209 (≠ H206), L235 (= L232), L236 (≠ I233)
- binding : S9 (= S12), T43 (= T46), D44 (= D47), R47 (= R50), V145 (= V142), R163 (≠ Q160), V166 (= V163), Y168 (≠ W165), E172 (≠ D169), V177 (= V174), K180 (≠ R177), S181 (= S178), Y187 (= Y184), E207 (≠ D204), E208 (≠ D205), W209 (≠ H206), L210 (= L207), V211 (≠ T208), R237 (≠ H234), K241 (≠ G238), M273 (≠ L270), G274 (= G271), E282 (= E278), R297 (≠ H293), P303 (≠ A299), V304 (≠ R300), K309 (= K305), W312 (≠ S308), R319 (= R315), P357 (≠ E360), R358 (= R361), R417 (= R422), L427 (= L432), Q432 (= Q437), R435 (= R440), L442 (≠ S447), E443 (≠ T448), T444 (≠ S449), G446 (= G451), L447 (≠ I452), F448 (= F453)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
42% identity, 98% coverage: 5:469/473 of query aligns to 2:464/468 of 1j09A
- active site: K246 (= K243)
- binding adenosine-5'-triphosphate: H15 (= H18), E208 (≠ D205), L235 (= L232), L236 (≠ I233), K243 (= K240), I244 (≠ L241), S245 (= S242), K246 (= K243), R247 (= R244)
- binding glutamic acid: R5 (= R8), A7 (= A10), S9 (= S12), E41 (= E44), Y187 (= Y184), N191 (≠ V188), R205 (= R202), W209 (≠ H206)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
42% identity, 98% coverage: 5:469/473 of query aligns to 2:464/468 of P27000
- R358 (= R361) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
45% identity, 79% coverage: 4:376/473 of query aligns to 2:353/380 of 4g6zA
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
36% identity, 98% coverage: 7:469/473 of query aligns to 4:476/485 of 4griB
- active site: S9 (= S12), K253 (= K243)
- binding glutamic acid: R5 (= R8), A7 (= A10), S9 (= S12), E41 (= E44), Y194 (= Y184), R212 (= R202), W216 (≠ H206)
- binding zinc ion: C105 (= C100), C107 (≠ A102), Y128 (= Y123), C132 (≠ W127)
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
41% identity, 96% coverage: 5:459/473 of query aligns to 3:464/485 of Q8DLI5
- R6 (= R8) binding L-glutamate
- Y192 (= Y184) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
41% identity, 96% coverage: 5:459/473 of query aligns to 2:463/484 of 2cfoA
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
37% identity, 98% coverage: 5:469/473 of query aligns to 103:556/564 of 3al0C
- active site: S110 (= S12), K335 (= K243)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R8), A108 (= A10), P109 (= P11), G118 (= G20), T122 (= T24), E142 (= E44), Y276 (= Y184), R294 (= R202), G295 (= G203), D297 (= D205), H298 (= H206), L324 (= L232), I325 (= I233), L333 (= L241)
- binding : T144 (= T46), D145 (= D47), R148 (= R50), Y208 (≠ A102), P213 (≠ E109), K252 (≠ Q160), M255 (≠ V163), I266 (≠ V174), K269 (≠ R177), S270 (= S178), Y276 (= Y184), D297 (= D205), H298 (= H206), L299 (= L207), S300 (≠ T208), N301 (= N209), K304 (≠ R212), R330 (≠ G238), P332 (≠ K240), G363 (= G271), W364 (= W272), R365 (≠ S273), E370 (= E278), S387 (≠ G295), K389 (≠ S297), V391 (≠ A299), I392 (≠ R300), K397 (= K305), W400 (≠ S308), R407 (= R315), E446 (= E360), K447 (≠ R361), Q453 (≠ E367), I457 (≠ G371), R509 (= R422), K520 (≠ G433), Q524 (= Q437), R527 (= R440), V535 (≠ T448), T536 (≠ S449), G538 (= G451), L539 (≠ I452)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
35% identity, 98% coverage: 5:469/473 of query aligns to 4:463/488 of 8vc5A
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid
34% identity, 99% coverage: 3:469/473 of query aligns to 1:495/502 of 6brlA
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
40% identity, 51% coverage: 6:247/473 of query aligns to 17:245/308 of P27305
- E55 (= E44) binding L-glutamate
- Y182 (= Y184) binding L-glutamate
- R200 (= R202) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
40% identity, 51% coverage: 6:248/473 of query aligns to 5:234/290 of 4a91A
- active site: S11 (= S12), K229 (= K243)
- binding glutamic acid: R7 (= R8), A9 (= A10), S11 (= S12), E43 (= E44), Y170 (= Y184), R188 (= R202), L192 (≠ H206)
- binding zinc ion: C99 (= C100), C101 (≠ A102), Y113 (= Y123), C117 (≠ D129)
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
32% identity, 64% coverage: 5:307/473 of query aligns to 11:316/455 of 3aiiA
8uk6A Candida albicans glutaminyl tRNA synthetase (gln4) in complex with n- pyrimidinyl-beta-thiophenylacrylamide (see paper)
36% identity, 23% coverage: 1:111/473 of query aligns to 35:144/573 of 8uk6A
Sites not aligning to the query:
Query Sequence
>WP_020175690.1 NCBI__GCF_000385335.1:WP_020175690.1
MSDPVVTRFAPSPTGFLHIGGARTALFNWLYACNTGGKMLLRIEDTDRERSTDAAIAAIL
DGLSWLGLTWDGDVIYQFQRAQRHREVAQSLLDTGKAYYCYATPKELEEMREKARAESRP
PRYDGRWRDRPASDAPPDVKPVIRLKAPQEGETVVEDKVQGRVSWANKDLDDLVLLRSDG
TPTYMLAVVVDDHDMGVTQIIRGDDHLTNAARQMQIYEALGWAVPAMAHIPLIHGPDGAK
LSKRHGALGVDAYRAMGYLPDALRNYLVRLGWSQGDKEFFSTQEMIEAFDLAHVGRSAAR
FDFVKLESMNGHYMRSSPDEDLLKALNAALPFLPNAEPILQKYDAAMRAKLLRAMPGLKE
RAKTLIELLDGANFLFAERPLPIDDKAKTILSPDGRTHLKQLIPRFAALPDWTAAATEEV
VRNYVEETKVKLGQVAQPLRAALTGRSTSPGIFDVLEVLGREESLSRLQDQSD
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory