SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_020177190.1 NCBI__GCF_000385335.1:WP_020177190.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
63% identity, 99% coverage: 4:1232/1242 of query aligns to 2:1225/1227 of P13009

query
sites
P13009

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C247 (= C248) binding Zn(2+)
C310 (= C311) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
C311 (= C312) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
E694 (= E693) binding methylcob(III)alamin
GDVHD 756:760 (= GDVHD 760:764) binding methylcob(III)alamin
D757 (= D761) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
H759 (= H763) binding axial binding residue; mutation to G: Loss of catalytic activity.
S804 (= S808) binding methylcob(III)alamin
T808 (= T812) binding methylcob(III)alamin
S810 (= S814) mutation to A: Decreases activity by about 40%.
A860 (= A865) binding methylcob(III)alamin
D946 (= D952) binding S-adenosyl-L-methionine
R1134 (= R1141) binding S-adenosyl-L-methionine
YY 1189:1190 (≠ YF 1196:1197) binding S-adenosyl-L-methionine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
54% identity, 98% coverage: 10:1232/1242 of query aligns to 22:1263/1265 of Q99707

query
sites
Q99707

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D

D

R61 (= R49) natural variant: R -> K
C255 (≠ T243) to Y: in dbSNP:rs1140598
GSR 382:384 (≠ GSA 368:370) binding (6S)-5,6,7,8-tetrahydrofolate
D449 (= D435) binding (6S)-5,6,7,8-tetrahydrofolate
N470 (= N456) binding (6S)-5,6,7,8-tetrahydrofolate
D537 (= D523) binding (6S)-5,6,7,8-tetrahydrofolate
N579 (= N565) binding (6S)-5,6,7,8-tetrahydrofolate
R585 (= R571) binding (6S)-5,6,7,8-tetrahydrofolate
R591 (= R577) binding (6S)-5,6,7,8-tetrahydrofolate
D919 (≠ A898) to G: in dbSNP:rs1805087
D963 (≠ T941) mutation to E: Decreases binding to MTRR; when associated with N-1071.
K1071 (= K1041) mutation to N: Decreases binding to MTRR; when associated with E-963.

3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
60% identity, 47% coverage: 653:1232/1242 of query aligns to 4:575/577 of 3bulA

query
sites
3bulA

W

W

R

R

S

S

E

W

P

E

V

V

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N

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Q

Q

V

V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

Y

Y

L

L

M

E

P

P

Y

F

M

I

E

E

E

A

E

S

K

K

R

E

K

Q

N

C

G

-

G

-

A

-

E

-

R

-

K

K

N

T

A

N

G

G

K

K

I

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G

G

K

K

N

N

I

I

V
|
V

G

G

V

V

V

V

L

L

A

Q

C

C

N

N

N

N

Y

Y

E

E

I

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

C

A

A

E

K

K

I

I

L

L

D

R

V

T

A

A

R

K

T

E

E

V

K

N

V

A

D

D

A

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

C

V

F

N

V

V

A

A

S

K

E

E

M

M

E

E

R

R

E

Q

G

G

F

F

S

T

M

I

P

P

L

L

L

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

R

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

N

E

P

Q

N

N

Y

Y

T

S

K

-

G

G

Q

P

T

T

V

V

Y

Y

V

V

T
x
Q

D
x
N

A
|
A

S

S

R

R

A
x
T

V

V

G

G

V

V

V

V

Q

A

N

A

L

L

F

L

S

S

P

D

A

T

A

Q

R

R

G

D

P

D

Y

F

I

V

E

A

A

R

I

T

K

R

T

K

E

E

Y

Y

E

E

N

T

V

V

A

R

E

I

A

Q

H

H

M

G

R

R

N

K

E

K

A

P

E

R

K

T

N

P

R

P

Q

V

P

T

L

L

P

E

R

A

A

A

R

R

A

D

N

N

A

D

L

F

K

A

L

F

D

D

W

W

A

Q

A

A

Y

Y

E

T

P

P

P

P

H

V

P

A

T

H

F

R

L

L

G

G

T

V

R

Q

I

E

F

V

R

E

T

A

Y

-

D

S

V

I

A

E

E

T

L

L

V

R

P

N

Y

Y

I

I

D

D

W

W

T

T

P

P

F

F

F

F

Q
x
M

T

T

W

W

E

S

M

L

R

A

G

G

R

K

Y

Y

P

P

D

R

I

I

L

L

S

E

D

D

P

E

E

V

K

V

G

G

A

V

A

E

A

A

Q

Q

Q

R

L

L

F

F

D

K

D

D

A

A

Q

N

A

D

M

M

L

L

A

D

R

K

I

L

V

S

E

A

E

E

H

K

W

T

F

L

D

N

P

P

K

R

A

G

V

V

I

V

G

G

F

L

W

F

P

P

A

A

N

N

A

R

I

V

G

G

D

D

D

D

I

I

K

E

L

I

Y

Y

T

R

G

D

E

E

S

T

R

R

S

T

E

H

V

V

L

I

A

N

T

V

F

S

H

H

T

H

L

L

R

R

Q

Q

Q

Q

L

-

S

T

K

E

R

K

D

T

G

G

R

F

P

A

N

N

I

Y

A

C

M

L

S

A

D

D

Y

F

I

V

A

A

P

P

V

K

E

L

S

S

L

G

K

K

P

A

D

D

Y

Y

L

I

G

G

G

A

F

F

V

A

V

V

T

T

A

G

G

G

A

L

R

E

E

E

E

D

K

A

I

L

A

A

D

D

R

A

F

F

A

E

K

A

A

Q

N

H

D

D

D

D

Y

Y

G

N

S

K

I

I

M

M

V

V

K

K

A

A

L

L

A

A

D
|
D

R

R

I

L

A

A

E

E

A

A

F

F

A

A

E

E

R

Y

M

L

H

H

E

E

R

R

V

V

R

R

K

K

E

V

F

Y

W

W

A

G

Y

Y

A

A

P

P

D

N

E

E

A

N

L

L

A

S

P

N

D

E

E

E

L

L

I

I

T

R

E

E

P

N

Y

Y

R

Q

G

G

I

I

R

R

P

P

A
|
A

P
|
P

G
|
G

Y
|
Y

P

P

A

A

Q

C

P

P

D

E

H
|
H

T

T

E

E

K
|
K

A

A

T

T

L

I

F

W

R

E

L

L

L

L

E

E

P

V

E

E

R

K

R

H

I

T

G

G

V

M

T

K

L

L

T

T

E

E

S

S

F

F

A

A

M
|
M

W

W

P

P

G
|
G

S

A

S

S

V
|
V

S
|
S

G

G

L

W

Y

Y

L

F

S

S

H

H

P

P

D

D

A

S

R

K

Y

Y

F

Y

G

A

V

V

A

A

K

Q

I

I

E

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

L

R

R

R

K

K

G

G

M

M

D

S

F

V

A

T

E

E

M

V

E

E

R

R

W

W

L

L

A

A

P

P

I

N

L

L

N

G

Y

Y

D

D

active site: D107 (= D761), H109 (= H763), S160 (= S814)
binding cobalamin: H109 (= H763), V116 (= V770), G152 (= G806), L153 (= L807), S154 (= S808), L156 (= L810), I157 (= I811), T158 (= T812), G183 (= G837), G184 (= G838), Q208 (≠ T863), N209 (≠ D864), A210 (= A865), T213 (≠ A868), M302 (≠ Q958), D443 (= D1100), A486 (= A1143), P487 (= P1144), G488 (= G1145), Y489 (= Y1146), H495 (= H1152), K498 (= K1155), M521 (= M1178), G524 (= G1181), V527 (= V1184), S528 (= S1185)

3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
60% identity, 47% coverage: 653:1232/1242 of query aligns to 4:575/576 of 3ivaA

query
sites
3ivaA

W

W

R

R

S

S

E

W

P

E

V

V

E

N

K

K

R

R

L

L

E

E

H

Y

A

S

L

L

V

V

N

K

G

G

I

I

T

T

E

E

F

F

I

I

D

E

A

Q

D

D

T

T

E

E

E

E

A

A

R

R

A

Q

K

Q

S

A

T

T

R

R

P

P

L

C

D

E

V

V

I

I

E

E

G

G

P

P

L

L

M

M

A

D

G

G

M

M

N

N

V

V

V

V

G

G

D

D

L

L

F

F

G

G

S

E

G

G

K

K

M

M

F

F

L

L

P

P

Q

Q

V

V

V

V

K

K

S

S

A

A

R

R

V

V

M

M

K

K

Q

Q

A

A

V

V

A

A

Y

Y

L

L

M

E

P

P

Y

F

M

I

E

E

E

A

E

S

K

K

R

E

K

Q

N

C

G

-

G

-

A

-

E

-

R

-

K

K

N

T

A

N

G

G

K

K

I

M

L

V

M

I

A

A

T

T

V

V

K

K

G

G

D
|
D

V

V

H
|
H

D

D

I

I

G
|
G

K

K

N

N

I

I

V
|
V

G

G

V

V

V

V

L

L

A

Q

C

C

N

N

N

N

Y

Y

E

E

I

I

I

V

D

D

L

L

G

G

V

V

M

M

V

V

P

P

C

A

A

E

K

K

I

I

L

L

D

R

V

T

A

A

R

K

T

E

E

V

K

N

V

A

D

D

A

L

I

I

G
|
G

L
|
L

S
|
S

G

G

L
|
L

I
|
I

T
|
T

P

P

S
|
S

L

L

D

D

E

E

M

M

C

V

F

N

V

V

A

A

S

K

E

E

M

M

E

E

R

R

E

Q

G

G

F

F

S

T

M

I

P

P

L

L

L

L

I

I

G
|
G

G
|
G

A

A

T

T

T

T

S

S

R

K

V

A

H

H

T

T

A

A

V

V

K

K

I

I

N

E

P

Q

N

N

Y

Y

T

S

K

-

G

G

Q

P

T

T

V

V

Y

Y

V

V

T
x
Q

D
x
N

A

A

S

S

R

R

A

T

V

V

G

G

V

V

V

V

Q

A

N

A

L

L

F

L

S

S

P

D

A

T

A

Q

R

R

G

D

P

D

Y

F

I

V

E

A

A

R

I

T

K

R

T

K

E

E

Y

Y

E

E

N

T

V

V

A

R

E

I

A

Q

H

H

M

G

R

R

N

K

E

K

A

P

E

R

K

T

N

P

R

P

Q

V

P

T

L

L

P

E

R

A

A

A

R

R

A

D

N

N

A

D

L

F

K

A

L

F

D

D

W

W

A

Q

A

A

Y

Y

E

T

P

P

P

P

H

V

P

A

T

H

F

R

L

L

G

G

T

V

R

Q

I

E

F

V

R

E

T

A

Y

-

D

S

V

I

A

E

E

T

L

L

V

R

P

N

Y

Y

I

I

D

D

W

W

T

T

P

P

F

F

F

F

Q

M

T
|
T

W

W

E

S

M

L

R

A

G

G

R

K

Y

Y

P

P

D

R

I

I

L

L

S

E

D

D

P

E

E

V

K

V

G

G

A

V

A

E

A

A

Q

Q

Q

R

L

L

F

F

D

K

D

D

A

A

Q

N

A

D

M

M

L

L

A

D

R

K

I

L

V

S

E

A

E

E

H

K

W

T

F

L

D

N

P

P

K

R

A

G

V

V

I

V

G

G

F

L

W

F

P

P

A

A

N

N

A

R

I

V

G

G

D

D

D

D

I

I

K

E

L

I

Y

Y

T

R

G

D

E

E

S

T

R

R

S

T

E

H

V

V

L

I

A

N

T

V

F

S

H

H

T

H

L

L

R

R

Q

Q

Q

Q

L

-

S

T

K

E

R

K

D

T

G

G

R

F

P

A

N

N

I

Y

A

C

M

L

S

A

D

D

Y

F

I

V

A

A

P

P

V

K

E

L

S

S

L

G

K

K

P

A

D

D

Y

Y

L

I

G

G

G

A

F

F

V

A

V

V

T

T

A

G

G

G

A

L

R

E

E

E

E

D

K

A

I

L

A

A

D

D

R

A

F

F

A

E

K

A

A

Q

N

H

D

D

D

D

Y

Y

G

N

S

K

I

I

M

M

V

V

K

K

A

A

L

L

A

A

D
|
D

R

R

I

L

A

A

E
|
E

A

A

F

F

A

A

E

E

R

Y

M

L

H

H

E

E

R

R

V

V

R

R

K

K

E

V

F

Y

W

W

A

G

Y

Y

A

A

P

P

D

N

E

E

A

N

L

L

A

S

P

N

D

E

E

E

L

L

I

I

T

R

E

E

P

N

Y

Y

R

Q

G

G

I

I

R
|
R

P
|
P

A
|
A

P

P

G
|
G

Y
|
Y

P

P

A
|
A

Q

C

P

P

D

E

H
|
H

T

T

E

E

K

K

A

A

T

T

L

I

F

W

R

E

L

L

L

L

E

E

P

V

E

E

R

K

R

H

I

T

G

G

V

M

T

K

L

L

T

T

E

E

S

S

F

F

A
|
A

M
|
M

W

W

P

P

G
|
G

S

A

S

S

V
|
V

S
|
S

G

G

L

W

Y

Y

L

F

S

S

H

H

P

P

D

D

A

S

R

K

Y
|
Y

F

Y

G

A

V

V

A

A

K

Q

I

I

E

Q

R

R

D

D

Q

Q

V

V

E

E

D

D

Y

Y

A

A

L

R

R

R

K

K

G

G

M

M

D

S

F

V

A

T

E

E

M

V

E

E

R

R

W

W

L

L

A

A

P

P

I

N

L

L

N

G

Y

Y

D

D

active site: D107 (= D761), H109 (= H763), S160 (= S814)
binding cobalamin: H109 (= H763), G112 (= G766), V116 (= V770), G152 (= G806), L153 (= L807), S154 (= S808), L156 (= L810), I157 (= I811), T158 (= T812), G183 (= G837), G184 (= G838), Q208 (≠ T863), N209 (≠ D864), T303 (= T959), D443 (= D1100), A486 (= A1143), G488 (= G1145), Y489 (= Y1146), H495 (= H1152), A520 (= A1177), M521 (= M1178), G524 (= G1181), V527 (= V1184), S528 (= S1185)
binding s-adenosyl-l-homocysteine: E447 (= E1104), R484 (= R1141), P485 (= P1142), Y489 (= Y1146), A491 (= A1148), Y539 (= Y1196)

4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
54% identity, 51% coverage: 10:637/1242 of query aligns to 6:611/611 of 4cczA

query
sites
4cczA

L

I

R

N

A

A

A

I

A

L

R

Q

E

K

K

R

I

I

L

M

V

V

L

L

D

D

G

G

A

G

M

M

G

G

T

T

M

M

I

I

Q

Q

Q

R

H

E

K

K

F

L

T

E

E

E

A

-

D

H

F

F

R

R

A

G

E

Q

R

E

F

F

A

K

D

D

W

H

P

A

R

R

D

P

L

L

R

K

G

G

N

N

N

N

D

D

L

I

L

L

V

S

L

I

T

T

Q

Q

P

P

H

D

A

V

I

I

R

Y

A

Q

I

I

H

H

L

K

A

E

Y

Y

C

-

D

-

A

L

G

L

A

A

D

D

I

I

I

I

A

E

T

T

N

N

T

T

F

F

S

S

S

S

T

T

R

S

I

I

A

A

Q

Q

A

A

D

D

Y

Y

G

G

M

L

E

E

R

H

L

L

V

A

P

Y

E

R

L

M

N

N

F

M

E

C

A

S

A

A

R

G

L

V

A

A

C

R

E

K

A

A

A

A

H

E

E

E

S

V

E

T

E

L

R

Q

H

T

G

G

R

I

R

K

H

R

F

F

V

V

A

A

G

G

A

A

L

L

G

G

P

P

T

T

N

N

R

K

T

T

A

L

S

S

I

V

S

S

P

P

D

S

V

V

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|
E

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|
G

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x
R

K

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|
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D

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N

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N

L

L

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V

V

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Y

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D

D

I

I

D

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P

K

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A

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C

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D

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L

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N

N

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E

G

A

T

T

E

E

R

K

L

L

L

L

A

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L

Y

A

A

E

Q

binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E362), G342 (= G368), R344 (≠ A370), N430 (= N456), M458 (= M484), D497 (= D523), G536 (= G562), S538 (= S564), N539 (= N565), F542 (= F568), R545 (= R571), R551 (= R577)

8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
37% identity, 70% coverage: 7:875/1242 of query aligns to 3:832/841 of 8g3hA

query
sites
8g3hA

F

F

A

P

A

Y

L

L

R

R

A

A

A

L

A

S

R

-

E

Q

K

R

I

V

L

L

V

V

L

F

D

D

G

G

A

A

M

M

G

G

T

T

M

E

I

L

Q

Q

Q

K

H

R

K

D

F

L

T

T

E

P

A

E

D

D

F

Y

R

G

A

E

E

E

R

A

F

Y

A

Y

D

G

W

C

P

P

R

-

D

-

L

-

R

-

G

-

N

-

N

-

D

E

L

V

L

L

V

N

L

R

T

T

Q

R

P

P

H

D

A

V

I

V

R

R

A

E

I

I

H

H

L

L

A

S

Y

Y

C

L

D

E

A

A

G

G

A

A

D

E

I

V

I

I

A

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T

T

N

N

T

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F

F

S

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S

A

T

L

R

R

I

H

A

V

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L

A

A

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Y

Y

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G

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L

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G

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L

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L

N

A

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Y

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R

A

A

A

A

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R

L

I

A

A

C

K

E

E

A

A

A

A

H

-

E

-

S

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-

E

E

R

P

H

Y

G

G

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R

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H

A

F

F

V

V

A

A

G

G

A

A

L

L

G

G

P

P

T

G

N

T

R

K

T

L

A

V

S

S

I

L

S

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P

-

D

-

V

-

N

-

N

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P

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G

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F

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R

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A

Q

V

I

S

S

F

W

D

E

E

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L

L

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D

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A

A

Y

Y

A

K

E

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A

A

A

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A

G

L

L

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G

G

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V

D

D

I

L

L

I

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A

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V

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A

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A

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M

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A

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L

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G

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V

K

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L

L

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P

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L

M

Q

I

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G

V

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F

T

-

D

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L

A

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T

G

G

R

T

T

M

L

L

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V

G

G

Q

T

T

D

P

E

T

Q

A

A

F

A

W

L

N

A

S

A

L

L

R

E

H

S

A

L

K

P

P

I

F

D

T

V

I

V

G

G

L

M

N

N

C

C

A

A

L

T

G

G

A

P

R

D

E

L

M

M

R

D

A

A

H

K

L

I

A

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Y

I

F

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A

R

Q

I

E

A

S

D

T

T

R

Y

F

V

V

C

A

A

C

Y

L

P

P

N

N

A

A

G

G

L

L

P

P

-

R

N

N

E

E

F

G

G

G

-

R

-

V

L

V

Y

Y

D

D

E

L

S

T

P

P

E

E

Y

E

M

L

A

A

S

R

L

W

I

H

G